Mitić, Nevena

Link to this page

Authority KeyName Variants
orcid::0000-0002-4056-0972
  • Mitić, Nevena (4)
Projects

Author's Bibliography

The procedure providing enhanced Agrobacterium-mediated transformation of wheat

Vinterhalter, Branka; Dodig, Dejan; Mitić, Nevena; Ninković, Slavica

(Walter De Gruyter Gmbh, Berlin, 2014)

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Dodig, Dejan
AU  - Mitić, Nevena
AU  - Ninković, Slavica
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/536
AB  - The examinations of conditions for establishing a variety independent Agrobacterium-mediated transformation procedure for wheat are preferable since many of cultivars and breeding lines remain recalcitrant for biotechnological manipulation, mainly due to low efficiency of plant regeneration in vitro, which is highly genotype specific. This paper describes and discusses an improved protocol for enhanced and low-genotype dependent Agrobacterium-mediated transformation using a super-binary vector LBA4404/pTOK233 carrying reporter gus-intron gene and hygromycin (hpt) and kanamicyn (nptII) selectable marker genes. The protocol was optimized on highly responsive common wheat cv. Vesna. Transient expression monitored by the gus-intron on explants after 3, 6 and 25 days of co-cultivation, followed by GUS expression and hygromycin resistance in whole plants indicated the protocol including a co-cultivation of freshly isolated immature embryos in the presence of ascorbic acid, and acetosyringone added only in the bacteria-containing infection medium combined with a delayed and stepwise increasing hygromycin B selection procedure significantly enhanced the transformation efficiency in cv. Vesna that exceed 7% of treated explants from previously 0.41%. Explant pre-cultivation did not additionally improve transformation efficiency. The optimized protocol was successful in evoking satisfactory transformation efficiencies from 3.6% to 10.8% in 5 less-responsive wheat genotypes. All 57 T0 hygromycin-resistant and GUS-positive lines were phenotypically normal and fertile. Therefore, the conditions employed in this study may serve as a base to facilitate the transformation in other, particularly recalcitrant wheat cultivars.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Biologia
T1  - The procedure providing enhanced Agrobacterium-mediated transformation of wheat
VL  - 69
IS  - 12
SP  - 1668
EP  - 1677
DO  - 10.2478/s11756-014-0477-2
ER  - 
@article{
author = "Vinterhalter, Branka and Dodig, Dejan and Mitić, Nevena and Ninković, Slavica",
year = "2014",
url = "http://rik.mrizp.rs/handle/123456789/536",
abstract = "The examinations of conditions for establishing a variety independent Agrobacterium-mediated transformation procedure for wheat are preferable since many of cultivars and breeding lines remain recalcitrant for biotechnological manipulation, mainly due to low efficiency of plant regeneration in vitro, which is highly genotype specific. This paper describes and discusses an improved protocol for enhanced and low-genotype dependent Agrobacterium-mediated transformation using a super-binary vector LBA4404/pTOK233 carrying reporter gus-intron gene and hygromycin (hpt) and kanamicyn (nptII) selectable marker genes. The protocol was optimized on highly responsive common wheat cv. Vesna. Transient expression monitored by the gus-intron on explants after 3, 6 and 25 days of co-cultivation, followed by GUS expression and hygromycin resistance in whole plants indicated the protocol including a co-cultivation of freshly isolated immature embryos in the presence of ascorbic acid, and acetosyringone added only in the bacteria-containing infection medium combined with a delayed and stepwise increasing hygromycin B selection procedure significantly enhanced the transformation efficiency in cv. Vesna that exceed 7% of treated explants from previously 0.41%. Explant pre-cultivation did not additionally improve transformation efficiency. The optimized protocol was successful in evoking satisfactory transformation efficiencies from 3.6% to 10.8% in 5 less-responsive wheat genotypes. All 57 T0 hygromycin-resistant and GUS-positive lines were phenotypically normal and fertile. Therefore, the conditions employed in this study may serve as a base to facilitate the transformation in other, particularly recalcitrant wheat cultivars.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Biologia",
title = "The procedure providing enhanced Agrobacterium-mediated transformation of wheat",
volume = "69",
number = "12",
pages = "1668-1677",
doi = "10.2478/s11756-014-0477-2"
}
Vinterhalter, B., Dodig, D., Mitić, N.,& Ninković, S. (2014). The procedure providing enhanced Agrobacterium-mediated transformation of wheat.
Biologia
Walter De Gruyter Gmbh, Berlin., 69(12), 1668-1677.
https://doi.org/10.2478/s11756-014-0477-2
Vinterhalter B, Dodig D, Mitić N, Ninković S. The procedure providing enhanced Agrobacterium-mediated transformation of wheat. Biologia. 2014;69(12):1668-1677
Vinterhalter Branka, Dodig Dejan, Mitić Nevena, Ninković Slavica, "The procedure providing enhanced Agrobacterium-mediated transformation of wheat" Biologia, 69, no. 12 (2014):1668-1677,
https://doi.org/10.2478/s11756-014-0477-2 .
2
3
2

Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant

Dodig, Dejan; Zorić, Miroslav; Lalević, Blazo; Šurlan-Momirović, Gordana; Mitić, Nevena; Nikolić, Radomirka; King, Stephen R.

(Univ Sao Paolo, Cerquera Cesar, 2010)

TY  - JOUR
AU  - Dodig, Dejan
AU  - Zorić, Miroslav
AU  - Lalević, Blazo
AU  - Šurlan-Momirović, Gordana
AU  - Mitić, Nevena
AU  - Nikolić, Radomirka
AU  - King, Stephen R.
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/339
AB  - Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR traits and to determine the period of wheat growth during which these factors were the most effective. The genotypes were grown in an experimental field during three seasons differing in meteorological conditions. The relationships between TCR traits and climatic factors within three time periods of wheat growth: 2, 6 and 10 weeks prior to embryo sampling were analysed by biplot analysis. The tissue culture traits were influenced at very different degrees by climatic factors: from 16.8% (RC) to 69.8% (CF). Donor plant environment with high temperatures and low rainfalls reduced (p  lt  0.05) the tissue culture performance of wheat genotypes. Callus formation was most sensitive to the temperature based factors. The environmental conditions between flowering and the medium milk stage were the most important for CF, while RC and PPE were not particularly related to any period.
PB  - Univ Sao Paolo, Cerquera Cesar
T2  - Scientia Agricola
T1  - Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant
VL  - 67
IS  - 3
SP  - 295
EP  - 300
DO  - 10.1590/S0103-90162010000300007
ER  - 
@article{
author = "Dodig, Dejan and Zorić, Miroslav and Lalević, Blazo and Šurlan-Momirović, Gordana and Mitić, Nevena and Nikolić, Radomirka and King, Stephen R.",
year = "2010",
url = "http://rik.mrizp.rs/handle/123456789/339",
abstract = "Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR traits and to determine the period of wheat growth during which these factors were the most effective. The genotypes were grown in an experimental field during three seasons differing in meteorological conditions. The relationships between TCR traits and climatic factors within three time periods of wheat growth: 2, 6 and 10 weeks prior to embryo sampling were analysed by biplot analysis. The tissue culture traits were influenced at very different degrees by climatic factors: from 16.8% (RC) to 69.8% (CF). Donor plant environment with high temperatures and low rainfalls reduced (p  lt  0.05) the tissue culture performance of wheat genotypes. Callus formation was most sensitive to the temperature based factors. The environmental conditions between flowering and the medium milk stage were the most important for CF, while RC and PPE were not particularly related to any period.",
publisher = "Univ Sao Paolo, Cerquera Cesar",
journal = "Scientia Agricola",
title = "Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant",
volume = "67",
number = "3",
pages = "295-300",
doi = "10.1590/S0103-90162010000300007"
}
Dodig, D., Zorić, M., Lalević, B., Šurlan-Momirović, G., Mitić, N., Nikolić, R.,& King, S. R. (2010). Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant.
Scientia Agricola
Univ Sao Paolo, Cerquera Cesar., 67(3), 295-300.
https://doi.org/10.1590/S0103-90162010000300007
Dodig D, Zorić M, Lalević B, Šurlan-Momirović G, Mitić N, Nikolić R, King SR. Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant. Scientia Agricola. 2010;67(3):295-300
Dodig Dejan, Zorić Miroslav, Lalević Blazo, Šurlan-Momirović Gordana, Mitić Nevena, Nikolić Radomirka, King Stephen R., "Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant" Scientia Agricola, 67, no. 3 (2010):295-300,
https://doi.org/10.1590/S0103-90162010000300007 .
1
3

Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes

Mitić, Nevena; Nikolić, Radomirka; Ninković, Slavica; Vinterhalter, D.; Vinterhalter, Branka; Dodig, Dejan

(Maik Nauka/Interperiodica/Springer, New York, 2009)

TY  - JOUR
AU  - Mitić, Nevena
AU  - Nikolić, Radomirka
AU  - Ninković, Slavica
AU  - Vinterhalter, D.
AU  - Vinterhalter, Branka
AU  - Dodig, Dejan
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/273
AB  - The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12-15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as large as the effect of the genotype, which should be taken into account when planning transformation work with wheat.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Russian Journal of Plant Physiology
T1  - Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes
VL  - 56
IS  - 4
SP  - 540
EP  - 545
DO  - 10.1134/S1021443709040141
ER  - 
@article{
author = "Mitić, Nevena and Nikolić, Radomirka and Ninković, Slavica and Vinterhalter, D. and Vinterhalter, Branka and Dodig, Dejan",
year = "2009",
url = "http://rik.mrizp.rs/handle/123456789/273",
abstract = "The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12-15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as large as the effect of the genotype, which should be taken into account when planning transformation work with wheat.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Russian Journal of Plant Physiology",
title = "Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes",
volume = "56",
number = "4",
pages = "540-545",
doi = "10.1134/S1021443709040141"
}
Mitić, N., Nikolić, R., Ninković, S., Vinterhalter, D., Vinterhalter, B.,& Dodig, D. (2009). Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes.
Russian Journal of Plant Physiology
Maik Nauka/Interperiodica/Springer, New York., 56(4), 540-545.
https://doi.org/10.1134/S1021443709040141
Mitić N, Nikolić R, Ninković S, Vinterhalter D, Vinterhalter B, Dodig D. Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes. Russian Journal of Plant Physiology. 2009;56(4):540-545
Mitić Nevena, Nikolić Radomirka, Ninković Slavica, Vinterhalter D., Vinterhalter Branka, Dodig Dejan, "Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes" Russian Journal of Plant Physiology, 56, no. 4 (2009):540-545,
https://doi.org/10.1134/S1021443709040141 .
5
2
3

Tissue culture and agronomic traits relationship in wheat

Dodig, Dejan; Zorić, Miroslav; Šurlan-Momirović, Gordana; Mitić, Nevena; Nikolić, Radomirka

(Springer, Dordrecht, 2008)

TY  - JOUR
AU  - Dodig, Dejan
AU  - Zorić, Miroslav
AU  - Šurlan-Momirović, Gordana
AU  - Mitić, Nevena
AU  - Nikolić, Radomirka
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/247
AB  - There are only a few reports about the relationship between tissue culture and agronomic traits in wheat. In an attempt to enlarge the knowledge in this area, 96 wheat genotypes of worldwide origin were evaluated for their tissue culture response as well as the agronomic performance in the three-year field trials. Plant regeneration was achieved from immature embryos collected 12-15 days after anthesis. Associations between tissue culture and agronomic traits were surveyed by the correlation and path coefficient analyses. A trait-association by year biplot was applied to understand environmental effects on these relationships. All significant trait-associations were of a moderate magnitude and were responsive to the environment. The path coefficient analysis revealed that a grain yield had the highest positive direct effect on a callus formation while the kernel number per spike had the highest positive direct effect on regenerative calli and plant number per embryo. Nevertheless, only productive tillering had significant (positive) direct effect on all studied tissue culture traits.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Tissue culture and agronomic traits relationship in wheat
VL  - 95
IS  - 1
SP  - 107
EP  - 114
DO  - 10.1007/s11240-008-9421-x
ER  - 
@article{
author = "Dodig, Dejan and Zorić, Miroslav and Šurlan-Momirović, Gordana and Mitić, Nevena and Nikolić, Radomirka",
year = "2008",
url = "http://rik.mrizp.rs/handle/123456789/247",
abstract = "There are only a few reports about the relationship between tissue culture and agronomic traits in wheat. In an attempt to enlarge the knowledge in this area, 96 wheat genotypes of worldwide origin were evaluated for their tissue culture response as well as the agronomic performance in the three-year field trials. Plant regeneration was achieved from immature embryos collected 12-15 days after anthesis. Associations between tissue culture and agronomic traits were surveyed by the correlation and path coefficient analyses. A trait-association by year biplot was applied to understand environmental effects on these relationships. All significant trait-associations were of a moderate magnitude and were responsive to the environment. The path coefficient analysis revealed that a grain yield had the highest positive direct effect on a callus formation while the kernel number per spike had the highest positive direct effect on regenerative calli and plant number per embryo. Nevertheless, only productive tillering had significant (positive) direct effect on all studied tissue culture traits.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Tissue culture and agronomic traits relationship in wheat",
volume = "95",
number = "1",
pages = "107-114",
doi = "10.1007/s11240-008-9421-x"
}
Dodig, D., Zorić, M., Šurlan-Momirović, G., Mitić, N.,& Nikolić, R. (2008). Tissue culture and agronomic traits relationship in wheat.
Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 95(1), 107-114.
https://doi.org/10.1007/s11240-008-9421-x
Dodig D, Zorić M, Šurlan-Momirović G, Mitić N, Nikolić R. Tissue culture and agronomic traits relationship in wheat. Plant Cell Tissue and Organ Culture. 2008;95(1):107-114
Dodig Dejan, Zorić Miroslav, Šurlan-Momirović Gordana, Mitić Nevena, Nikolić Radomirka, "Tissue culture and agronomic traits relationship in wheat" Plant Cell Tissue and Organ Culture, 95, no. 1 (2008):107-114,
https://doi.org/10.1007/s11240-008-9421-x .
11
10
9