Milivojević, Marija

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  • Milivojević, Marija (25)

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Non-parametric yield stability analysis of zp maize hybrids in serbia

Branković-Radojčić, Dragana V.; Babić, Vojka; Petrović, Tanja; Milivojević, Marija; Jovanović, Snežana; Popović, Aleksandar; Srdić, Jelena

(Belgrade : Maize Research Institute, Zemun Polje, 2022)

TY  - CONF
AU  - Branković-Radojčić, Dragana V.
AU  - Babić, Vojka
AU  - Petrović, Tanja
AU  - Milivojević, Marija
AU  - Jovanović, Snežana
AU  - Popović, Aleksandar
AU  - Srdić, Jelena
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/954
AB  - High and stabile yield in different production environments is priority in maize breeding. New 
statistical methods are constantly being sought to accompany analysis of variance, in order to 
achieve more reliable hybrid assessment. In this study non-parametric stability analysis is 
applied in order to assess GxE interaction for yield of 36 commercial maize hybrids. The 
experiment was set up at five locations in Serbia for three years according to the RCBD design 
in three replications. Yield stability of investigated genotypes was analysed by stability 
parameters Si(1), Si(2), Si(3), Si(6), TOP and RS. Analysis of variance identified highly significant 
F values for all experimental factors. Bredenkamp method confirmed the existence of non crossover GxE interaction, for maize yield. Hybrid ZPH15 achieved the most stable yield based 
on parameters Si(1) and Si(2). According to parameter Si(3) it was ZPH5, while based on 
parameter Si(6) it was ZPH34. The highest overall yield achieved ZPH36 (11.18 t/ha), which 
was quite unstable (rank 24 in parameters Si(1) and Si(2)), and very unstable (rank 34 in 
parameters Si(3) and Si(6)). The most stable hybrids had average yields. In total, the hybrid 
ZPH23 had the best average rank (15.93). Based on TOP parameters, ZPH36 had the best rank 
(yield), followed by ZPH11, ZPH20, ZPH21 and ZPH9. However, RS parameter, revealed that 
ZPH21 was the most stable hybrid, so taking into account both TOP and RS parameters this is 
the most productive and the most stable hybrid. Based on this research, TOP and RS are the 
best parameters for selecting new maize hybrids for production in particular environment. In 
case of identical TOP value, the genotype with the lowest RS value should be selected. The 
parameters Si(1), Si(2), Si(3) and Si(6) can be used as alternative methods for the selection of 
genotypes with moderate yield and high stability.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Non-parametric yield stability analysis of zp maize hybrids in serbia
EP  - 46
EP  - 46
ER  - 
@conference{
author = "Branković-Radojčić, Dragana V. and Babić, Vojka and Petrović, Tanja and Milivojević, Marija and Jovanović, Snežana and Popović, Aleksandar and Srdić, Jelena",
year = "2022",
abstract = "High and stabile yield in different production environments is priority in maize breeding. New 
statistical methods are constantly being sought to accompany analysis of variance, in order to 
achieve more reliable hybrid assessment. In this study non-parametric stability analysis is 
applied in order to assess GxE interaction for yield of 36 commercial maize hybrids. The 
experiment was set up at five locations in Serbia for three years according to the RCBD design 
in three replications. Yield stability of investigated genotypes was analysed by stability 
parameters Si(1), Si(2), Si(3), Si(6), TOP and RS. Analysis of variance identified highly significant 
F values for all experimental factors. Bredenkamp method confirmed the existence of non crossover GxE interaction, for maize yield. Hybrid ZPH15 achieved the most stable yield based 
on parameters Si(1) and Si(2). According to parameter Si(3) it was ZPH5, while based on 
parameter Si(6) it was ZPH34. The highest overall yield achieved ZPH36 (11.18 t/ha), which 
was quite unstable (rank 24 in parameters Si(1) and Si(2)), and very unstable (rank 34 in 
parameters Si(3) and Si(6)). The most stable hybrids had average yields. In total, the hybrid 
ZPH23 had the best average rank (15.93). Based on TOP parameters, ZPH36 had the best rank 
(yield), followed by ZPH11, ZPH20, ZPH21 and ZPH9. However, RS parameter, revealed that 
ZPH21 was the most stable hybrid, so taking into account both TOP and RS parameters this is 
the most productive and the most stable hybrid. Based on this research, TOP and RS are the 
best parameters for selecting new maize hybrids for production in particular environment. In 
case of identical TOP value, the genotype with the lowest RS value should be selected. The 
parameters Si(1), Si(2), Si(3) and Si(6) can be used as alternative methods for the selection of 
genotypes with moderate yield and high stability.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Non-parametric yield stability analysis of zp maize hybrids in serbia",
pages = "46-46"
}
Branković-Radojčić, D. V., Babić, V., Petrović, T., Milivojević, M., Jovanović, S., Popović, A.,& Srdić, J.. (2022). Non-parametric yield stability analysis of zp maize hybrids in serbia. in 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje..
Branković-Radojčić DV, Babić V, Petrović T, Milivojević M, Jovanović S, Popović A, Srdić J. Non-parametric yield stability analysis of zp maize hybrids in serbia. in 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;:null-46..
Branković-Radojčić, Dragana V., Babić, Vojka, Petrović, Tanja, Milivojević, Marija, Jovanović, Snežana, Popović, Aleksandar, Srdić, Jelena, "Non-parametric yield stability analysis of zp maize hybrids in serbia" in 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022).

Analiza trenda u procesu ispitivanja mase 1000 semena primenom brojača

Petrović, Tanja; Vujinovic, Jasna; Milivojević, Marija; Branković-Radojčić, Dragana V.; Vukadinović, Radmila

(Novi Sad : društvo za procesnu tehniku i energetiku u poljoprivredi, 2022)

TY  - JOUR
AU  - Petrović, Tanja
AU  - Vujinovic, Jasna
AU  - Milivojević, Marija
AU  - Branković-Radojčić, Dragana V.
AU  - Vukadinović, Radmila
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/972
AB  - Praćenje i kontrola rada laboratorijske opreme je jedan od zahteva standarda za laboratorije za 
ispitivanje semena. Period i način kontrole zavise od učestalosti korišćenja opreme, njene strarosti itd. U Laboratoriji za ispitivanje semena Instituta za kukuruz „Zemun Polje“, brojač semena koristi se u ispitivanju mase 1000 semena radi ubrzanja procesa rada i jednom godišnje se redovno proverava njegova tačnost brojanja. Međutim, u dosadašnjoj praksi nije se obraćala pažnja da li prilikom rada brojača dolazi do selektivnog izbora semena koje se broji. Preciznost i slučajan izbor semena kod ispitivanja mase 1000 semena u laboratoriji je od velike važnosti kako bi se obezbedila reprezentativnost uzorka i ispravno donela odluka o daljem procesu pakovanja. Određivanje mase semena prema ISTA Pravilima se može izvesti na dva načina: brojanjem cele frakcije čistog semena i brojanje ponavljanja od po 100 semena. U oba slučaja brojanje se može izvesti ručno ili korišćenjem brojača. Cilj ovog istraživanja je provera da li laboratorijski brojač semena u toku svog rada, u zavisnosti od brzine, selektuje seme po obliku i veličini i da li postoji trend. U radu je ispitivano 10 vrsta različite krupnoće semena (Capsicum annuum, peletirano seme Beta vulgaris, Brassica napus, Medicago sativa, Raphanus sativus, Sinapis alba, Triticum aestivum, Helianthus annuus, Glycine max i Zea mays). Brojač je podešen na dve brzine brojanja (maksimalna brzina automatski zadata i 50% od maksimalne brzine). Brojano je 10 ponavljanja po 100 semena. Posle svakog ponavljanja izbrojanog na brojaču, ručno je proveravan broj semena i merena je masa. Analizom dobijenih rezultata utvrđeno je da brojač precizno broji, a na osnovu rezultata izmerene mase uočeno je da se na maksimalnoj brzini pojavljuje trend prilikom rada brojača kod vrsta Capsicum annuum i Glycine max (selektovanje semena od sitnijeg ka krupnijem). Kod semena Medicago sativa i peletiranog semena Beta vulgaris, uočen je trend selektovanja semena od krupnijeg ka sitnijem. Prilikom sporijeg brojanja (50% od maksimalne 
brzine), ne pojavljuje se trend prilikom rada brojača, kod gore navedenih vrsta semena. Za ostale vrste nije uočen trend (proseci mase 1000 semena za obe posmatrane brzine su skoro identični). Rezultati ovih analiza ukazuju na važnost provere opreme kako u smislu tačnosti brojanja tako i selektovanja semena u cilju dobijanja tačnih rezultata ispitivanja mase 1000 semena.
AB  - Accuracy and random selection of seeds when testing the 1000 seeds weight in the laboratory is of 
great importance in order to ensure the representativeness of the sample and precise information for 
further packaging process. The aim of this research was to check whether the laboratory seed counter 
selects seeds by shape and size and whether there is a trend during operation at maximum and reduced 
speed. Ten species of different seed sizes were used. Ten replicates of 100 seeds were counted by 
counter, the number of seeds was manually checked and the weight measured. The analysis of the 
obtained results showed that the counter is precise, but weight results showed selection of smaller seeds 
(Capsicum annuum and Glycine max) at maximum counting speed. Trend of larger seed selection was 
observed in Medicago sativa and pelleted Beta vulgaris. Slower counting (50% of the maximum 
speed), ensured precise counting without trends.
PB  - Novi Sad : društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - 34 Nacionalna konferencija procesna tehnika i energetika u poljoprivredi PTEP 03-08. april 2022 - Zbornik radova
T1  - Analiza trenda u procesu ispitivanja mase 1000 semena primenom brojača
T1  - Trend analysis in 1000 seed weight testing using a counter
SP  - 48
EP  - 53
ER  - 
@article{
author = "Petrović, Tanja and Vujinovic, Jasna and Milivojević, Marija and Branković-Radojčić, Dragana V. and Vukadinović, Radmila",
year = "2022",
abstract = "Praćenje i kontrola rada laboratorijske opreme je jedan od zahteva standarda za laboratorije za 
ispitivanje semena. Period i način kontrole zavise od učestalosti korišćenja opreme, njene strarosti itd. U Laboratoriji za ispitivanje semena Instituta za kukuruz „Zemun Polje“, brojač semena koristi se u ispitivanju mase 1000 semena radi ubrzanja procesa rada i jednom godišnje se redovno proverava njegova tačnost brojanja. Međutim, u dosadašnjoj praksi nije se obraćala pažnja da li prilikom rada brojača dolazi do selektivnog izbora semena koje se broji. Preciznost i slučajan izbor semena kod ispitivanja mase 1000 semena u laboratoriji je od velike važnosti kako bi se obezbedila reprezentativnost uzorka i ispravno donela odluka o daljem procesu pakovanja. Određivanje mase semena prema ISTA Pravilima se može izvesti na dva načina: brojanjem cele frakcije čistog semena i brojanje ponavljanja od po 100 semena. U oba slučaja brojanje se može izvesti ručno ili korišćenjem brojača. Cilj ovog istraživanja je provera da li laboratorijski brojač semena u toku svog rada, u zavisnosti od brzine, selektuje seme po obliku i veličini i da li postoji trend. U radu je ispitivano 10 vrsta različite krupnoće semena (Capsicum annuum, peletirano seme Beta vulgaris, Brassica napus, Medicago sativa, Raphanus sativus, Sinapis alba, Triticum aestivum, Helianthus annuus, Glycine max i Zea mays). Brojač je podešen na dve brzine brojanja (maksimalna brzina automatski zadata i 50% od maksimalne brzine). Brojano je 10 ponavljanja po 100 semena. Posle svakog ponavljanja izbrojanog na brojaču, ručno je proveravan broj semena i merena je masa. Analizom dobijenih rezultata utvrđeno je da brojač precizno broji, a na osnovu rezultata izmerene mase uočeno je da se na maksimalnoj brzini pojavljuje trend prilikom rada brojača kod vrsta Capsicum annuum i Glycine max (selektovanje semena od sitnijeg ka krupnijem). Kod semena Medicago sativa i peletiranog semena Beta vulgaris, uočen je trend selektovanja semena od krupnijeg ka sitnijem. Prilikom sporijeg brojanja (50% od maksimalne 
brzine), ne pojavljuje se trend prilikom rada brojača, kod gore navedenih vrsta semena. Za ostale vrste nije uočen trend (proseci mase 1000 semena za obe posmatrane brzine su skoro identični). Rezultati ovih analiza ukazuju na važnost provere opreme kako u smislu tačnosti brojanja tako i selektovanja semena u cilju dobijanja tačnih rezultata ispitivanja mase 1000 semena., Accuracy and random selection of seeds when testing the 1000 seeds weight in the laboratory is of 
great importance in order to ensure the representativeness of the sample and precise information for 
further packaging process. The aim of this research was to check whether the laboratory seed counter 
selects seeds by shape and size and whether there is a trend during operation at maximum and reduced 
speed. Ten species of different seed sizes were used. Ten replicates of 100 seeds were counted by 
counter, the number of seeds was manually checked and the weight measured. The analysis of the 
obtained results showed that the counter is precise, but weight results showed selection of smaller seeds 
(Capsicum annuum and Glycine max) at maximum counting speed. Trend of larger seed selection was 
observed in Medicago sativa and pelleted Beta vulgaris. Slower counting (50% of the maximum 
speed), ensured precise counting without trends.",
publisher = "Novi Sad : društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "34 Nacionalna konferencija procesna tehnika i energetika u poljoprivredi PTEP 03-08. april 2022 - Zbornik radova",
title = "Analiza trenda u procesu ispitivanja mase 1000 semena primenom brojača, Trend analysis in 1000 seed weight testing using a counter",
pages = "48-53"
}
Petrović, T., Vujinovic, J., Milivojević, M., Branković-Radojčić, D. V.,& Vukadinović, R.. (2022). Analiza trenda u procesu ispitivanja mase 1000 semena primenom brojača. in 34 Nacionalna konferencija procesna tehnika i energetika u poljoprivredi PTEP 03-08. april 2022 - Zbornik radova
Novi Sad : društvo za procesnu tehniku i energetiku u poljoprivredi., 48-53.
Petrović T, Vujinovic J, Milivojević M, Branković-Radojčić DV, Vukadinović R. Analiza trenda u procesu ispitivanja mase 1000 semena primenom brojača. in 34 Nacionalna konferencija procesna tehnika i energetika u poljoprivredi PTEP 03-08. april 2022 - Zbornik radova. 2022;:48-53..
Petrović, Tanja, Vujinovic, Jasna, Milivojević, Marija, Branković-Radojčić, Dragana V., Vukadinović, Radmila, "Analiza trenda u procesu ispitivanja mase 1000 semena primenom brojača" in 34 Nacionalna konferencija procesna tehnika i energetika u poljoprivredi PTEP 03-08. april 2022 - Zbornik radova (2022):48-53.

Identification of early decline of seed quality by vigor tests

Petrović, Tanja; Milivojević, Marija; Branković-Radojčić, Dragana V.; Jovanović, Snežana; Vujinović, Jasna; Vukadinović, Radmila; Stojadinović-Životić, Jasmina

(Belgrade : Maize Research Institute, Zemun Polje, 2022)

TY  - CONF
AU  - Petrović, Tanja
AU  - Milivojević, Marija
AU  - Branković-Radojčić, Dragana V.
AU  - Jovanović, Snežana
AU  - Vujinović, Jasna
AU  - Vukadinović, Radmila
AU  - Stojadinović-Životić, Jasmina
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/953
AB  - Seed vigor is the quality parameter which indicates germination under wide range of 
environmental conditions. This trait can be identified by standard germination test as so-called 
first count germination or germination energy. In addition, several tests for vigor evaluation 
were adopted by seed testing associations such as ISTA and AOSA. The main purpose for 
developing additional vigor tests is to obtain fast and reliable information on seed vigor which 
contributes to decision making in the inventory management. ISTA rules prescribes several 
tests for evaluating seed vigor, namely: conductivity test, accelerated ageing (AA) for soybean, 
controlled deterioration for Brassica seeds, Radicle emergence (RE) and Tetrazolium (TZ) 
vigor test for soybean. Seed testing laboratory of the Maize Research Institute is accredited by 
ISTA for RE vigor test, has internally developed cold-test and is capable for application of TZ 
vigor test for research purposes. These tests were applied on several maize seeds lots with 
declining germination energy and still highly rated final germination. Results of vigor tests 
were correlated with the standard germination quality parameters, germination energy and final 
count, in order to identify vigor test which provides the most approximate output to the standard 
germination test. Correlations were significant for all vigor tests, in average 0.882 and 0.713 
for germination first and final count, respectively. The highest compatibility between vigor and 
germination tests was identified for cold test with correlation coefficients of 0.952 and 0.839 
for germination first and final count, respectively. However, due to duration of cold test (2 
weeks), it is not capable of providing fast information. Therefore, other two vigor tests, TZ and 
RE, which are shorter and have high correlation with germination energy (0.901 and 0.811, 
respectively) and final count (0.713 and 0.728, respectively) can be recommended for this 
purpose.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Identification of early decline of seed quality by vigor tests
SP  - 61
EP  - 61
ER  - 
@conference{
author = "Petrović, Tanja and Milivojević, Marija and Branković-Radojčić, Dragana V. and Jovanović, Snežana and Vujinović, Jasna and Vukadinović, Radmila and Stojadinović-Životić, Jasmina",
year = "2022",
abstract = "Seed vigor is the quality parameter which indicates germination under wide range of 
environmental conditions. This trait can be identified by standard germination test as so-called 
first count germination or germination energy. In addition, several tests for vigor evaluation 
were adopted by seed testing associations such as ISTA and AOSA. The main purpose for 
developing additional vigor tests is to obtain fast and reliable information on seed vigor which 
contributes to decision making in the inventory management. ISTA rules prescribes several 
tests for evaluating seed vigor, namely: conductivity test, accelerated ageing (AA) for soybean, 
controlled deterioration for Brassica seeds, Radicle emergence (RE) and Tetrazolium (TZ) 
vigor test for soybean. Seed testing laboratory of the Maize Research Institute is accredited by 
ISTA for RE vigor test, has internally developed cold-test and is capable for application of TZ 
vigor test for research purposes. These tests were applied on several maize seeds lots with 
declining germination energy and still highly rated final germination. Results of vigor tests 
were correlated with the standard germination quality parameters, germination energy and final 
count, in order to identify vigor test which provides the most approximate output to the standard 
germination test. Correlations were significant for all vigor tests, in average 0.882 and 0.713 
for germination first and final count, respectively. The highest compatibility between vigor and 
germination tests was identified for cold test with correlation coefficients of 0.952 and 0.839 
for germination first and final count, respectively. However, due to duration of cold test (2 
weeks), it is not capable of providing fast information. Therefore, other two vigor tests, TZ and 
RE, which are shorter and have high correlation with germination energy (0.901 and 0.811, 
respectively) and final count (0.713 and 0.728, respectively) can be recommended for this 
purpose.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Identification of early decline of seed quality by vigor tests",
pages = "61-61"
}
Petrović, T., Milivojević, M., Branković-Radojčić, D. V., Jovanović, S., Vujinović, J., Vukadinović, R.,& Stojadinović-Životić, J.. (2022). Identification of early decline of seed quality by vigor tests. in 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 61-61.
Petrović T, Milivojević M, Branković-Radojčić DV, Jovanović S, Vujinović J, Vukadinović R, Stojadinović-Životić J. Identification of early decline of seed quality by vigor tests. in 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;:61-61..
Petrović, Tanja, Milivojević, Marija, Branković-Radojčić, Dragana V., Jovanović, Snežana, Vujinović, Jasna, Vukadinović, Radmila, Stojadinović-Životić, Jasmina, "Identification of early decline of seed quality by vigor tests" in 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022):61-61.

Monitoring of seed counter in 1000 seed weight testing

Milivojević, Marija; Vujinović, Jasna; Branković-Radojčić, Dragana V.; Vukadinović, Radmila; Petrović, Tanja

(Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, 2022)

TY  - JOUR
AU  - Milivojević, Marija
AU  - Vujinović, Jasna
AU  - Branković-Radojčić, Dragana V.
AU  - Vukadinović, Radmila
AU  - Petrović, Tanja
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/899
AB  - Accuracy and random selection of seeds when testing the 1000 seeds weight in the laboratory is of great importance in order to ensure the representativeness of the sample and precise information for further packaging process. The aim of this research was to check whether the laboratory seed counter selects seeds by shape and size and whether there is a trend during operation at maximum and reduced speed. Ten species of different seed sizes were used. Ten replicates of 100 seeds were counted by counter, the number of seeds was manually checked and the weight measured. The analysis of the obtained results showed that the counter is precise, but weight results showed selection of smaller seeds (Capsicum annuum and Glycine max) at maximum counting speed. Trend of larger seed selection was observed in Medicago sativa and pelleted Beta vulgaris. Slower counting (50% of the maximum speed), is ensured precise counting without trends.
AB  - Praćenje i kontrola rada laboratorijske opreme je jedan od zahteva akreditacionih standarda za laboratorije za ispitivanje
semena. U dosadašnjoj praksi nije se obraćala pažnja da li prilikom rada brojača dolazi do selektivnog izbora semena koje se broji.
Preciznost i slučajan izbor semena kod ispitivanja mase 1000 semena u laboratoriji je od velike važnosti kako bi se obezbedila
reprezentativnost uzorka. Cilj ovog istraživanja je provera da li laboratorijski brojač semena u toku svog rada, pri maksimalnoj i
smanjenoj brzini selektuje seme po obliku i veličini i da li postoji trend. U radu je ispitivano 10 vrsta različite krupno će semena
(Capsicum annuum, peletirano seme Beta vulgaris, Brassica napus, Medicago sativa, Raphanus sativus, Sinapis alba, Triticum
aestivum, Helianthus annuus, Glycine max i Zea mays). Analizom dobijenih rezultata utvrđeno je da brojač precizno broji. Na osnovu
rezultata izmerene mase uočeno je da se na maksimalnoj brzini brojanja pojavljuje trend selektovanja prvo sitnijeg, a zatim krupnijeg
semena kod vrsta Capsicum annuum i Glycine max. Nasuprot tome, kod semena Medicago sativa i peletiranog semena Beta vulgaris,
uočen je trend selektovanja semena od krupnijeg ka sitnijem. Prilikom sporijeg brojanja (50% od maksimalne brzine), postiže se
slučajan odabir semena tj. ne pojavljuje se trend prilikom rada brojača, kod gore navedenih vrsta semena. Za ostale vrste nije uočen
trend postepenog smanjenja niti pove ćanja vrednosti mase u okviru ispitivanih 10 ponavljanja. Rezultati ovih analiza ukazuju na
važnost provere opreme kako u smislu tačnosti brojanja tako i selektovanja semena u cilju dobijanja tačnih rezultata ispitivanja mase
1000 semena.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
T2  - Journal on Processing and Energy in Agriculture
T1  - Monitoring of seed counter in 1000 seed weight testing
T1  - Provera brojača semena u ispitivanju mase 1000 semena
VL  - 26
IS  - 1
SP  - 34
EP  - 37
EP  - 
DO  - 10.5937/jpea26-36855
ER  - 
@article{
author = "Milivojević, Marija and Vujinović, Jasna and Branković-Radojčić, Dragana V. and Vukadinović, Radmila and Petrović, Tanja",
year = "2022",
abstract = "Accuracy and random selection of seeds when testing the 1000 seeds weight in the laboratory is of great importance in order to ensure the representativeness of the sample and precise information for further packaging process. The aim of this research was to check whether the laboratory seed counter selects seeds by shape and size and whether there is a trend during operation at maximum and reduced speed. Ten species of different seed sizes were used. Ten replicates of 100 seeds were counted by counter, the number of seeds was manually checked and the weight measured. The analysis of the obtained results showed that the counter is precise, but weight results showed selection of smaller seeds (Capsicum annuum and Glycine max) at maximum counting speed. Trend of larger seed selection was observed in Medicago sativa and pelleted Beta vulgaris. Slower counting (50% of the maximum speed), is ensured precise counting without trends., Praćenje i kontrola rada laboratorijske opreme je jedan od zahteva akreditacionih standarda za laboratorije za ispitivanje
semena. U dosadašnjoj praksi nije se obraćala pažnja da li prilikom rada brojača dolazi do selektivnog izbora semena koje se broji.
Preciznost i slučajan izbor semena kod ispitivanja mase 1000 semena u laboratoriji je od velike važnosti kako bi se obezbedila
reprezentativnost uzorka. Cilj ovog istraživanja je provera da li laboratorijski brojač semena u toku svog rada, pri maksimalnoj i
smanjenoj brzini selektuje seme po obliku i veličini i da li postoji trend. U radu je ispitivano 10 vrsta različite krupno će semena
(Capsicum annuum, peletirano seme Beta vulgaris, Brassica napus, Medicago sativa, Raphanus sativus, Sinapis alba, Triticum
aestivum, Helianthus annuus, Glycine max i Zea mays). Analizom dobijenih rezultata utvrđeno je da brojač precizno broji. Na osnovu
rezultata izmerene mase uočeno je da se na maksimalnoj brzini brojanja pojavljuje trend selektovanja prvo sitnijeg, a zatim krupnijeg
semena kod vrsta Capsicum annuum i Glycine max. Nasuprot tome, kod semena Medicago sativa i peletiranog semena Beta vulgaris,
uočen je trend selektovanja semena od krupnijeg ka sitnijem. Prilikom sporijeg brojanja (50% od maksimalne brzine), postiže se
slučajan odabir semena tj. ne pojavljuje se trend prilikom rada brojača, kod gore navedenih vrsta semena. Za ostale vrste nije uočen
trend postepenog smanjenja niti pove ćanja vrednosti mase u okviru ispitivanih 10 ponavljanja. Rezultati ovih analiza ukazuju na
važnost provere opreme kako u smislu tačnosti brojanja tako i selektovanja semena u cilju dobijanja tačnih rezultata ispitivanja mase
1000 semena.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad : National Society of Processing and Energy in Agriculture",
journal = "Journal on Processing and Energy in Agriculture",
title = "Monitoring of seed counter in 1000 seed weight testing, Provera brojača semena u ispitivanju mase 1000 semena",
volume = "26",
number = "1",
pages = "34-37-",
doi = "10.5937/jpea26-36855"
}
Milivojević, M., Vujinović, J., Branković-Radojčić, D. V., Vukadinović, R.,& Petrović, T.. (2022). Monitoring of seed counter in 1000 seed weight testing. in Journal on Processing and Energy in Agriculture
Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi., 26(1), 34-37.
https://doi.org/10.5937/jpea26-36855
Milivojević M, Vujinović J, Branković-Radojčić DV, Vukadinović R, Petrović T. Monitoring of seed counter in 1000 seed weight testing. in Journal on Processing and Energy in Agriculture. 2022;26(1):34-37.
doi:10.5937/jpea26-36855 .
Milivojević, Marija, Vujinović, Jasna, Branković-Radojčić, Dragana V., Vukadinović, Radmila, Petrović, Tanja, "Monitoring of seed counter in 1000 seed weight testing" in Journal on Processing and Energy in Agriculture, 26, no. 1 (2022):34-37,
https://doi.org/10.5937/jpea26-36855 . .

Application of standard germination and vigour tests for seed quality assessment of maize inbred lines

Milivojević, Marija; Srdić, Jelena; Filipović, Milomir; Petrović, Tanja; Branković-Radojčić, Dragana V.; Marković, Ksenija; Boćanski, Jan

(Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije, 2021)

TY  - JOUR
AU  - Milivojević, Marija
AU  - Srdić, Jelena
AU  - Filipović, Milomir
AU  - Petrović, Tanja
AU  - Branković-Radojčić, Dragana V.
AU  - Marković, Ksenija
AU  - Boćanski, Jan
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/924
AB  - Information regarding the adaptation of maize (Zea mays L.) inbred lines to various field 
conditions and seed longevity is essential for seed companies. The objective of this work was to 
evaluate different vigour tests for detecting differences among naturally aged seeds and to assess 
the relationship between seed quality parameters and pedigree data of maize inbred lines. A seed 
of 15 maize inbred lines, selected at Maize Research Institute Zemun Polje, belonging to different 
heterotic groups was produced during 2011. Seed quality was assessed in 2015 by standard ger mination, radical emergence, accelerated ageing, and cold test. Root length of 4-day old seedlings 
was measured in all applied tests. Analysis of variance showed that genotype and storage condi tions had a significant effect on all seed quality parameters. Large variation among inbred lines 
was detected in seed vigour determined by three tests, in particular by the accelerated ageing test.
Cold test results showed very good chilling tolerance of tested genotypes, except two inbred lines 
with Lancaster background. The correlation between the cold test and the accelerated ageing test 
was significant, while the radical emergence test had a poor correlation with them. Obtained 
results indicate that the application of either cold test or accelerated ageing test is reliable for 
screening seed vigour of maize inbred lines.
AB  - Semenskim kompanijama su od izuzetne važnosti informacije o adaptibilnosti samooplod-
nih linija kukuruza (Zea mays L ) različitim uslovima u polju i dugovečnosti semena Cilj ovog
rada bio je da se izvrši procena vigor testova za otkrivanje razlika između prirodno ostarelog
semena u različitim uslovima čuvanja i da se proceni odnos između parametara kvaliteta semena
i podataka o pedigreu samooplodnih linija kukuruza Seme 15 samooplodnih linija kukuruza
selekcionisanih u “Institutu za kukuruz” - Zemun Polje, koje pripadaju različitim heterotičnim
grupama, proizvedeno je 2011 godine Kvalitet semena je ocenjivan 2015 godine primenom
standardnog testa za klijavost, testa za pojavu primarnog korena, ubrzanim starenjem i hlad-
nim testom U svim primenjenim ispitivanjima merena je dužina korena klijanaca starih 4 dana
Analiza varijanse je pokazala da genotip i uslovi čuvanja semena imaju značajan uticaj na sve
parametre kvaliteta semena Samooplodne linije kukuruza su se značajno razlikovale u pogledu
vigora semena na osnovu tri primenjena testa, a naročito testa ubrzanog starenja Rezultati hlad-
nog testa su pokazali visoku tolerantnost ispitivanih genotipova na niske temperature prilikom
nicanja, osim dve samooplodne linije Lancaster porekla Korelacija između hladnog testa i testa
ubrzanog starenja bila je značajna, dok je test pojave primarnog korena imao slabu korelaciju sa
njima Dobijeni rezultati ukazuju da je primena hladnog testa i testa ubrzanog starenja pouzdana
za skrining vigora semena samooplodnih linija kukuruza
PB  - Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - Application of standard germination and vigour tests for seed quality assessment of maize inbred lines
T1  - Primena testova standardne klijavosti i vigor testova u proceni kvaliteta semena samooplodnih linija kukuruza
VL  - 27
IS  - 2
SP  - 35
EP  - 45
DO  - 10.5937/SelSem2102035M
ER  - 
@article{
author = "Milivojević, Marija and Srdić, Jelena and Filipović, Milomir and Petrović, Tanja and Branković-Radojčić, Dragana V. and Marković, Ksenija and Boćanski, Jan",
year = "2021",
abstract = "Information regarding the adaptation of maize (Zea mays L.) inbred lines to various field 
conditions and seed longevity is essential for seed companies. The objective of this work was to 
evaluate different vigour tests for detecting differences among naturally aged seeds and to assess 
the relationship between seed quality parameters and pedigree data of maize inbred lines. A seed 
of 15 maize inbred lines, selected at Maize Research Institute Zemun Polje, belonging to different 
heterotic groups was produced during 2011. Seed quality was assessed in 2015 by standard ger mination, radical emergence, accelerated ageing, and cold test. Root length of 4-day old seedlings 
was measured in all applied tests. Analysis of variance showed that genotype and storage condi tions had a significant effect on all seed quality parameters. Large variation among inbred lines 
was detected in seed vigour determined by three tests, in particular by the accelerated ageing test.
Cold test results showed very good chilling tolerance of tested genotypes, except two inbred lines 
with Lancaster background. The correlation between the cold test and the accelerated ageing test 
was significant, while the radical emergence test had a poor correlation with them. Obtained 
results indicate that the application of either cold test or accelerated ageing test is reliable for 
screening seed vigour of maize inbred lines., Semenskim kompanijama su od izuzetne važnosti informacije o adaptibilnosti samooplod-
nih linija kukuruza (Zea mays L ) različitim uslovima u polju i dugovečnosti semena Cilj ovog
rada bio je da se izvrši procena vigor testova za otkrivanje razlika između prirodno ostarelog
semena u različitim uslovima čuvanja i da se proceni odnos između parametara kvaliteta semena
i podataka o pedigreu samooplodnih linija kukuruza Seme 15 samooplodnih linija kukuruza
selekcionisanih u “Institutu za kukuruz” - Zemun Polje, koje pripadaju različitim heterotičnim
grupama, proizvedeno je 2011 godine Kvalitet semena je ocenjivan 2015 godine primenom
standardnog testa za klijavost, testa za pojavu primarnog korena, ubrzanim starenjem i hlad-
nim testom U svim primenjenim ispitivanjima merena je dužina korena klijanaca starih 4 dana
Analiza varijanse je pokazala da genotip i uslovi čuvanja semena imaju značajan uticaj na sve
parametre kvaliteta semena Samooplodne linije kukuruza su se značajno razlikovale u pogledu
vigora semena na osnovu tri primenjena testa, a naročito testa ubrzanog starenja Rezultati hlad-
nog testa su pokazali visoku tolerantnost ispitivanih genotipova na niske temperature prilikom
nicanja, osim dve samooplodne linije Lancaster porekla Korelacija između hladnog testa i testa
ubrzanog starenja bila je značajna, dok je test pojave primarnog korena imao slabu korelaciju sa
njima Dobijeni rezultati ukazuju da je primena hladnog testa i testa ubrzanog starenja pouzdana
za skrining vigora semena samooplodnih linija kukuruza",
publisher = "Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "Application of standard germination and vigour tests for seed quality assessment of maize inbred lines, Primena testova standardne klijavosti i vigor testova u proceni kvaliteta semena samooplodnih linija kukuruza",
volume = "27",
number = "2",
pages = "35-45",
doi = "10.5937/SelSem2102035M"
}
Milivojević, M., Srdić, J., Filipović, M., Petrović, T., Branković-Radojčić, D. V., Marković, K.,& Boćanski, J.. (2021). Application of standard germination and vigour tests for seed quality assessment of maize inbred lines. in Selekcija i semenarstvo
Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije., 27(2), 35-45.
https://doi.org/10.5937/SelSem2102035M
Milivojević M, Srdić J, Filipović M, Petrović T, Branković-Radojčić DV, Marković K, Boćanski J. Application of standard germination and vigour tests for seed quality assessment of maize inbred lines. in Selekcija i semenarstvo. 2021;27(2):35-45.
doi:10.5937/SelSem2102035M .
Milivojević, Marija, Srdić, Jelena, Filipović, Milomir, Petrović, Tanja, Branković-Radojčić, Dragana V., Marković, Ksenija, Boćanski, Jan, "Application of standard germination and vigour tests for seed quality assessment of maize inbred lines" in Selekcija i semenarstvo, 27, no. 2 (2021):35-45,
https://doi.org/10.5937/SelSem2102035M . .

High quality seed as the ultimate goal

Petrović, Tanja; Milivojević, Marija; Branković-Radojčić, Dragana V.; Jovanović, Snežana; Babić, Vojka

(Belgrade-Zemun : Maize Research Institute, Zemun Polje, 2021)

TY  - CONF
AU  - Petrović, Tanja
AU  - Milivojević, Marija
AU  - Branković-Radojčić, Dragana V.
AU  - Jovanović, Snežana
AU  - Babić, Vojka
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/922
AB  - High quality seed can be defined as the ability of seed to germinate 
vigorously and provide normal seedling and uniform crop establishment 
under wide range of environmental conditions. This complex trait results 
from a sound genetic background, good practice during seed production, 
favorable environment during development on the mother plant, optimal time 
of harvest, appropriate processing and storage. To identify seed quality 
marker(s) which will predict the seed quality has been a challenging task for 
seed researchers for a long time which has not been successfully 
accomplished yet. Therefore, testing germination and other traits in seed 
testing laboratories is still the only reliable source of information on seed 
quality. For commercial testing are developed standard methods aiming to 
provide good prediction of seedling establishment in the field. Very often, 
there are several standard methods developed for testing seed germination of 
one plant species. They can differ in germination substrate or temperature 
applied during the testing. In most cases, irrespective on testing conditions, 
seed quality will not differ significantly; however, at seed lots with declining 
quality testing conditions applied can be highly discriminative. Since the 
seed quality depends on more than one factor, it is understandable that it is 
not targeted in the breeding programs, however, efforts should be constant in 
identifying the impact of genetics on this trait and be focused on varieties 
which have potential in providing high seed quality.
PB  - Belgrade-Zemun : Maize Research Institute, Zemun Polje
C3  - International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9. June 2021. - Book of abstracts
T1  - High quality seed as the ultimate goal
SP  - 82
ER  - 
@conference{
author = "Petrović, Tanja and Milivojević, Marija and Branković-Radojčić, Dragana V. and Jovanović, Snežana and Babić, Vojka",
year = "2021",
abstract = "High quality seed can be defined as the ability of seed to germinate 
vigorously and provide normal seedling and uniform crop establishment 
under wide range of environmental conditions. This complex trait results 
from a sound genetic background, good practice during seed production, 
favorable environment during development on the mother plant, optimal time 
of harvest, appropriate processing and storage. To identify seed quality 
marker(s) which will predict the seed quality has been a challenging task for 
seed researchers for a long time which has not been successfully 
accomplished yet. Therefore, testing germination and other traits in seed 
testing laboratories is still the only reliable source of information on seed 
quality. For commercial testing are developed standard methods aiming to 
provide good prediction of seedling establishment in the field. Very often, 
there are several standard methods developed for testing seed germination of 
one plant species. They can differ in germination substrate or temperature 
applied during the testing. In most cases, irrespective on testing conditions, 
seed quality will not differ significantly; however, at seed lots with declining 
quality testing conditions applied can be highly discriminative. Since the 
seed quality depends on more than one factor, it is understandable that it is 
not targeted in the breeding programs, however, efforts should be constant in 
identifying the impact of genetics on this trait and be focused on varieties 
which have potential in providing high seed quality.",
publisher = "Belgrade-Zemun : Maize Research Institute, Zemun Polje",
journal = "International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9. June 2021. - Book of abstracts",
title = "High quality seed as the ultimate goal",
pages = "82"
}
Petrović, T., Milivojević, M., Branković-Radojčić, D. V., Jovanović, S.,& Babić, V.. (2021). High quality seed as the ultimate goal. in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9. June 2021. - Book of abstracts
Belgrade-Zemun : Maize Research Institute, Zemun Polje., 82.
Petrović T, Milivojević M, Branković-Radojčić DV, Jovanović S, Babić V. High quality seed as the ultimate goal. in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9. June 2021. - Book of abstracts. 2021;:82..
Petrović, Tanja, Milivojević, Marija, Branković-Radojčić, Dragana V., Jovanović, Snežana, Babić, Vojka, "High quality seed as the ultimate goal" in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9. June 2021. - Book of abstracts (2021):82.

Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions

Božić, Manja; Stanojević, Aleksandra; Marković, Ksenija; Ignjatović-Micić, Dragana; Nikolić, Dragana; Milivojević, Marija; Nikolić, Ana

(Belgrade : Serbian Genetics Society, 2021)

TY  - JOUR
AU  - Božić, Manja
AU  - Stanojević, Aleksandra
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Dragana
AU  - Milivojević, Marija
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/832
AB  - Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance.
AB  - Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions
VL  - 53
IS  - 3
SP  - 1147
EP  - 1165
DO  - 10.2298/GENSR2103147B
ER  - 
@article{
author = "Božić, Manja and Stanojević, Aleksandra and Marković, Ksenija and Ignjatović-Micić, Dragana and Nikolić, Dragana and Milivojević, Marija and Nikolić, Ana",
year = "2021",
abstract = "Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance., Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions",
volume = "53",
number = "3",
pages = "1147-1165",
doi = "10.2298/GENSR2103147B"
}
Božić, M., Stanojević, A., Marković, K., Ignjatović-Micić, D., Nikolić, D., Milivojević, M.,& Nikolić, A.. (2021). Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika
Belgrade : Serbian Genetics Society., 53(3), 1147-1165.
https://doi.org/10.2298/GENSR2103147B
Božić M, Stanojević A, Marković K, Ignjatović-Micić D, Nikolić D, Milivojević M, Nikolić A. Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika. 2021;53(3):1147-1165.
doi:10.2298/GENSR2103147B .
Božić, Manja, Stanojević, Aleksandra, Marković, Ksenija, Ignjatović-Micić, Dragana, Nikolić, Dragana, Milivojević, Marija, Nikolić, Ana, "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions" in Genetika, 53, no. 3 (2021):1147-1165,
https://doi.org/10.2298/GENSR2103147B . .

Antioxidants from maize seeds and accelerated ageing

Kravić, Natalija; Dragičević, Vesna; Milivojević, Marija; Babić, Vojka; Žilić, Slađana

(Beograd : Društvo selekcionara i semenara Republike Srbije, 2021)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Dragičević, Vesna
AU  - Milivojević, Marija
AU  - Babić, Vojka
AU  - Žilić, Slađana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/839
AB  - Seed deterioration is expressed as the loss of viability, vigour and quality during ageing The
major causes of seed deterioration refer to free radical-mediated lipid peroxidation, enzyme
inactivation or protein degradation, disruption of cellular membranes and damage to genetic
integrity Accelerated ageing (AA) is a simple method for studying the mechanisms of deterio-
ration over short periods In order to estimate the deterioration rate, seeds of four maize geno-
types were exposed to accelerated ageing for different time points (three and six days of AA) In
parallel, the same genotypes were evaluated regarding the content of total phenolics, phenolic
acids, flavonoids and anthocyanins, as well as regarding total antioxidant capacity Compared
to non-stressed seeds, ageing was evidenced by decreased germination energy (48 9%), a total
number of seedlings (40 8%) and seedlings growth (70 0% for root length, 44 0% and 10 5% for
root and shoot fresh weight, 5 0% for seed rest fresh weight, 12 0% and 11 5% for root and shoot
dry weight, 15 3% for seed rest dry weight, respectively), as well as by an increased number of
abnormal seedlings (22 0%) Correlation analysis revealed that higher content of total phenolics,
flavonoids and anthocyanins contributed to a higher radical scavenging activity, which was re-
flected through a lower level of deterioration rate, both regarding the energy of germination and
overall seedling performance.
AB  - Propadanje semena se ogleda u gubitku životne sposobnosti, energije i kvaliteta Dejstvo slo-
bodnih radikala dovodi do peroksidacije lipida, inaktivacije enzima, degradacije proteina, raza-
ranja ćelijskih membrana i oštećenja genetičkog materijala, i smatra se glavnim uzrokom propa-
danja semena Ubrzano starenje, kao metod, omogućava proučavanje mehanizama propadanja
semena u kratkom vremenskom periodu Za procenu stepena propadanja, seme četiri genotipa
kukuruza je izloženo tretmanu ubrzanog starenja u trajanju od tri i šest dana Uporedo sa ovim,
kod istih genotipova je ispitivan sadržaj ukupnih fenola, fenolnih kiselina, flavonoida i antoci-
jana, kao i ukupni antioksidativni kapacitet Poređenjem sa nestresiranim semenom (kontrola),
starenje je rezultiralo padom energije klijavosti (48,9%), smanjenjem ukupnog broja klijanaca
(40,8%), smanjenjem rasta klijanaca (70,0% za dužinu korena, 44,0% i 10,5% za svežu masu ko-
rena i izdanka, 5,0% za svežu masu semenog ostatka, 12 0% i 11 5% za suvu masu korena i iz -
danka, 15 3% za suvu masu semenog ostatka, respektivno), kao i povećanjem broja nenormalnih
klijanaca (22,0%) Korelacionom analizom je utvrđeno da je povećani sadržaj ukupnih fenola,
flavonoida i antocijana doprineo većoj sposobnosti gušenja slobodnih radikala, reflektujući se
kroz smanjen stepen propadanja semena, kako u pogledu energije klijavosti, tako i u pogledu
ukupne performanse klijanca.
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - Antioxidants from maize seeds and accelerated ageing
VL  - 27
IS  - 2
SP  - 47
EP  - 57
DO  - 10.5937/SelSem2102047K
ER  - 
@article{
author = "Kravić, Natalija and Dragičević, Vesna and Milivojević, Marija and Babić, Vojka and Žilić, Slađana",
year = "2021",
abstract = "Seed deterioration is expressed as the loss of viability, vigour and quality during ageing The
major causes of seed deterioration refer to free radical-mediated lipid peroxidation, enzyme
inactivation or protein degradation, disruption of cellular membranes and damage to genetic
integrity Accelerated ageing (AA) is a simple method for studying the mechanisms of deterio-
ration over short periods In order to estimate the deterioration rate, seeds of four maize geno-
types were exposed to accelerated ageing for different time points (three and six days of AA) In
parallel, the same genotypes were evaluated regarding the content of total phenolics, phenolic
acids, flavonoids and anthocyanins, as well as regarding total antioxidant capacity Compared
to non-stressed seeds, ageing was evidenced by decreased germination energy (48 9%), a total
number of seedlings (40 8%) and seedlings growth (70 0% for root length, 44 0% and 10 5% for
root and shoot fresh weight, 5 0% for seed rest fresh weight, 12 0% and 11 5% for root and shoot
dry weight, 15 3% for seed rest dry weight, respectively), as well as by an increased number of
abnormal seedlings (22 0%) Correlation analysis revealed that higher content of total phenolics,
flavonoids and anthocyanins contributed to a higher radical scavenging activity, which was re-
flected through a lower level of deterioration rate, both regarding the energy of germination and
overall seedling performance., Propadanje semena se ogleda u gubitku životne sposobnosti, energije i kvaliteta Dejstvo slo-
bodnih radikala dovodi do peroksidacije lipida, inaktivacije enzima, degradacije proteina, raza-
ranja ćelijskih membrana i oštećenja genetičkog materijala, i smatra se glavnim uzrokom propa-
danja semena Ubrzano starenje, kao metod, omogućava proučavanje mehanizama propadanja
semena u kratkom vremenskom periodu Za procenu stepena propadanja, seme četiri genotipa
kukuruza je izloženo tretmanu ubrzanog starenja u trajanju od tri i šest dana Uporedo sa ovim,
kod istih genotipova je ispitivan sadržaj ukupnih fenola, fenolnih kiselina, flavonoida i antoci-
jana, kao i ukupni antioksidativni kapacitet Poređenjem sa nestresiranim semenom (kontrola),
starenje je rezultiralo padom energije klijavosti (48,9%), smanjenjem ukupnog broja klijanaca
(40,8%), smanjenjem rasta klijanaca (70,0% za dužinu korena, 44,0% i 10,5% za svežu masu ko-
rena i izdanka, 5,0% za svežu masu semenog ostatka, 12 0% i 11 5% za suvu masu korena i iz -
danka, 15 3% za suvu masu semenog ostatka, respektivno), kao i povećanjem broja nenormalnih
klijanaca (22,0%) Korelacionom analizom je utvrđeno da je povećani sadržaj ukupnih fenola,
flavonoida i antocijana doprineo većoj sposobnosti gušenja slobodnih radikala, reflektujući se
kroz smanjen stepen propadanja semena, kako u pogledu energije klijavosti, tako i u pogledu
ukupne performanse klijanca.",
publisher = "Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "Antioxidants from maize seeds and accelerated ageing",
volume = "27",
number = "2",
pages = "47-57",
doi = "10.5937/SelSem2102047K"
}
Kravić, N., Dragičević, V., Milivojević, M., Babić, V.,& Žilić, S.. (2021). Antioxidants from maize seeds and accelerated ageing. in Selekcija i semenarstvo
Beograd : Društvo selekcionara i semenara Republike Srbije., 27(2), 47-57.
https://doi.org/10.5937/SelSem2102047K
Kravić N, Dragičević V, Milivojević M, Babić V, Žilić S. Antioxidants from maize seeds and accelerated ageing. in Selekcija i semenarstvo. 2021;27(2):47-57.
doi:10.5937/SelSem2102047K .
Kravić, Natalija, Dragičević, Vesna, Milivojević, Marija, Babić, Vojka, Žilić, Slađana, "Antioxidants from maize seeds and accelerated ageing" in Selekcija i semenarstvo, 27, no. 2 (2021):47-57,
https://doi.org/10.5937/SelSem2102047K . .

Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes

Tabaković, Marijenka; Simić, Milena; Stanisavljević, Rade; Milivojević, Marija; Sečanski, Mile; Poštić, Dobrivoj

(Santiago : Instituto de Investigaciones Agropecuarias, INIA, 2020)

TY  - JOUR
AU  - Tabaković, Marijenka
AU  - Simić, Milena
AU  - Stanisavljević, Rade
AU  - Milivojević, Marija
AU  - Sečanski, Mile
AU  - Poštić, Dobrivoj
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/808
AB  - The uniform seed material is a prerequisite of stable yields. Therefore, the aim of the study was to observe variability of physiological seed traits depending on the classification of seeds by size and shape, and to determine advantages of large over small seed fractions. Three maize (Zea mays L.) hybrids (ZP 505, ZP 677, ZP 684), produced in two locations (Orahovo, Plavna), were  classified into six fractions; small flat seed (SFS), medium small flat seed (MSFS), large flat
seed (LFS), small round seed (SRS), medium small round seed (MSRS) and large round seed (LRS). Two laboratory treatments were applied on seed: TR1 (cold test) and TR2 (20/30 ºC). In both temperature treatments, the first evaluation was done after 72-h germination, and then every 24 h until 7th day. The highest total germination (G) was recorded for ZP 677 (93.7%), location Orahovo (94.9%), temperature TR2 (95.2%) and MSFS fraction (89.7%). The total proportion of
factors in the variance for G was R2  = 0.694. Locations and genotypes predominantly affected the germination rate (GR), η = 0.338. Flat fraction seeds had more rapid emergence (> 90%) of seedlings than the round fraction seeds (> 85%). Round seeds were more susceptible and seed size had a greater effect on vigour than LRS, 70%. The partial effect of the fraction on G was not estimated (η = 0.037), but its cumulative effect with other factors was evident (Hybrid × Fraction,
η = 0.070).
PB  - Santiago : Instituto de Investigaciones Agropecuarias, INIA
T2  - Chilean Journal of Agricultural Research
T1  - Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes
VL  - 80
IS  - 3
SP  - 381
EP  - 392
DO  - 10.4067/S0718-58392020000300381
ER  - 
@article{
author = "Tabaković, Marijenka and Simić, Milena and Stanisavljević, Rade and Milivojević, Marija and Sečanski, Mile and Poštić, Dobrivoj",
year = "2020",
abstract = "The uniform seed material is a prerequisite of stable yields. Therefore, the aim of the study was to observe variability of physiological seed traits depending on the classification of seeds by size and shape, and to determine advantages of large over small seed fractions. Three maize (Zea mays L.) hybrids (ZP 505, ZP 677, ZP 684), produced in two locations (Orahovo, Plavna), were  classified into six fractions; small flat seed (SFS), medium small flat seed (MSFS), large flat
seed (LFS), small round seed (SRS), medium small round seed (MSRS) and large round seed (LRS). Two laboratory treatments were applied on seed: TR1 (cold test) and TR2 (20/30 ºC). In both temperature treatments, the first evaluation was done after 72-h germination, and then every 24 h until 7th day. The highest total germination (G) was recorded for ZP 677 (93.7%), location Orahovo (94.9%), temperature TR2 (95.2%) and MSFS fraction (89.7%). The total proportion of
factors in the variance for G was R2  = 0.694. Locations and genotypes predominantly affected the germination rate (GR), η = 0.338. Flat fraction seeds had more rapid emergence (> 90%) of seedlings than the round fraction seeds (> 85%). Round seeds were more susceptible and seed size had a greater effect on vigour than LRS, 70%. The partial effect of the fraction on G was not estimated (η = 0.037), but its cumulative effect with other factors was evident (Hybrid × Fraction,
η = 0.070).",
publisher = "Santiago : Instituto de Investigaciones Agropecuarias, INIA",
journal = "Chilean Journal of Agricultural Research",
title = "Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes",
volume = "80",
number = "3",
pages = "381-392",
doi = "10.4067/S0718-58392020000300381"
}
Tabaković, M., Simić, M., Stanisavljević, R., Milivojević, M., Sečanski, M.,& Poštić, D.. (2020). Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes. in Chilean Journal of Agricultural Research
Santiago : Instituto de Investigaciones Agropecuarias, INIA., 80(3), 381-392.
https://doi.org/10.4067/S0718-58392020000300381
Tabaković M, Simić M, Stanisavljević R, Milivojević M, Sečanski M, Poštić D. Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes. in Chilean Journal of Agricultural Research. 2020;80(3):381-392.
doi:10.4067/S0718-58392020000300381 .
Tabaković, Marijenka, Simić, Milena, Stanisavljević, Rade, Milivojević, Marija, Sečanski, Mile, Poštić, Dobrivoj, "Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes" in Chilean Journal of Agricultural Research, 80, no. 3 (2020):381-392,
https://doi.org/10.4067/S0718-58392020000300381 . .
9
5

Standard germination and seedling emergence of maize inbred lines in different temperature conditions

Petrović, Tanja; Radosavljević, Nebojša; Babić, Milosav; Milivojević, Marija; Vukadinović, Radmila; Branković-Radojčić, Dragana V.

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2019)

TY  - JOUR
AU  - Petrović, Tanja
AU  - Radosavljević, Nebojša
AU  - Babić, Milosav
AU  - Milivojević, Marija
AU  - Vukadinović, Radmila
AU  - Branković-Radojčić, Dragana V.
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/745
AB  - Standard laboratory germination seed test provides information on seed performance under defined environmental conditions aiming to estimate field planting value. Purpose of this study was to compare results from the standard germination test with seedling emergence in different outside temperature conditions. Sowing was done in three periods during spring, in trays exposed to outside conditions. Data obtained showed that differences in the germination, detected in the standard laboratory test, were also confirmed in field conditions. Expression of these differences, however, was dependent on temperature conditions during different sowing periods. Low temperatures during the early sowing were more discriminative than later temperature increase which completely reduced differences detected in the standard germination test.
AB  - Standardni test klijavosti pruža informacije o ponašanju semena u definisanim uslovima klijanja sa ciljem procene poljske setvene vrednosti. Svrha ovog istraživanja bila je poređenje rezultata standardnog testa klijavosti sa nicanjem klijanaca u polju pri različitim spoljašnjim temperaturnim uslovima. Setva je obavljena tokom tri vremenska perioda u proleće, u posudama izloženim spoljašnjim uslovima. Dobijeni rezultati su pokazali da su razlike u klijavosti, detektovane pomoću standardnog laboratorijskog testa, potvrđene i u sub-optimalnim spoljnim uslovima. Međutim, ispoljavanje ovih razlika zavisilo je od temperaturnih uslova tokom različitih setvenih perioda. Niske temperature u ranom periodu setve su bile diskriminativnije od kasnijeg porasta temperature koji je potpuno umanjio razlike detektovane u standardom testu klijavosti.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Standard germination and seedling emergence of maize inbred lines in different temperature conditions
T1  - Standardna klijavost i nicanje klijanaca samooplodnih linija kukuruza u različitim temperaturnim uslovima
VL  - 25
IS  - 1
SP  - 9
EP  - 14
DO  - 10.5937/SelSem1901009P
UR  - conv_2001
ER  - 
@article{
author = "Petrović, Tanja and Radosavljević, Nebojša and Babić, Milosav and Milivojević, Marija and Vukadinović, Radmila and Branković-Radojčić, Dragana V.",
year = "2019",
abstract = "Standard laboratory germination seed test provides information on seed performance under defined environmental conditions aiming to estimate field planting value. Purpose of this study was to compare results from the standard germination test with seedling emergence in different outside temperature conditions. Sowing was done in three periods during spring, in trays exposed to outside conditions. Data obtained showed that differences in the germination, detected in the standard laboratory test, were also confirmed in field conditions. Expression of these differences, however, was dependent on temperature conditions during different sowing periods. Low temperatures during the early sowing were more discriminative than later temperature increase which completely reduced differences detected in the standard germination test., Standardni test klijavosti pruža informacije o ponašanju semena u definisanim uslovima klijanja sa ciljem procene poljske setvene vrednosti. Svrha ovog istraživanja bila je poređenje rezultata standardnog testa klijavosti sa nicanjem klijanaca u polju pri različitim spoljašnjim temperaturnim uslovima. Setva je obavljena tokom tri vremenska perioda u proleće, u posudama izloženim spoljašnjim uslovima. Dobijeni rezultati su pokazali da su razlike u klijavosti, detektovane pomoću standardnog laboratorijskog testa, potvrđene i u sub-optimalnim spoljnim uslovima. Međutim, ispoljavanje ovih razlika zavisilo je od temperaturnih uslova tokom različitih setvenih perioda. Niske temperature u ranom periodu setve su bile diskriminativnije od kasnijeg porasta temperature koji je potpuno umanjio razlike detektovane u standardom testu klijavosti.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Standard germination and seedling emergence of maize inbred lines in different temperature conditions, Standardna klijavost i nicanje klijanaca samooplodnih linija kukuruza u različitim temperaturnim uslovima",
volume = "25",
number = "1",
pages = "9-14",
doi = "10.5937/SelSem1901009P",
url = "conv_2001"
}
Petrović, T., Radosavljević, N., Babić, M., Milivojević, M., Vukadinović, R.,& Branković-Radojčić, D. V.. (2019). Standard germination and seedling emergence of maize inbred lines in different temperature conditions. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 25(1), 9-14.
https://doi.org/10.5937/SelSem1901009P
conv_2001
Petrović T, Radosavljević N, Babić M, Milivojević M, Vukadinović R, Branković-Radojčić DV. Standard germination and seedling emergence of maize inbred lines in different temperature conditions. in Selekcija i semenarstvo. 2019;25(1):9-14.
doi:10.5937/SelSem1901009P
conv_2001 .
Petrović, Tanja, Radosavljević, Nebojša, Babić, Milosav, Milivojević, Marija, Vukadinović, Radmila, Branković-Radojčić, Dragana V., "Standard germination and seedling emergence of maize inbred lines in different temperature conditions" in Selekcija i semenarstvo, 25, no. 1 (2019):9-14,
https://doi.org/10.5937/SelSem1901009P .,
conv_2001 .
1

Povećanje kvaliteta semena paprike primenom mikorize

Poštić, Dobrivoj; Štrbanović, Ratibor; Stanojković-Sebić, Aleksandra; Tabaković, Marijenka; Milivojević, Marija; Jovanović, V. Snežana; Stanisavljević, Rade

(Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad, 2019)

TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Štrbanović, Ratibor
AU  - Stanojković-Sebić, Aleksandra
AU  - Tabaković, Marijenka
AU  - Milivojević, Marija
AU  - Jovanović, V. Snežana
AU  - Stanisavljević, Rade
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/739
AB  - The purpose of this paper is to evaluate the effect of the year of cultivation, seed population and mycorrhizal seed treatment on two most important indicators of the pepper seed quality, namely germination energy and total germination. The pepper seed quality parameters, i.e. the first count and the total germination rate, were examined in the period 2017-2018. The results obtained show significant differences (p   < 0.01) between the parameter values under consideration relative to the year of cultivation (Factor A), seed population (Factor B) and mycorrhizal sees treatment (Factor C). In the first experimental year, there was an increase in the first count and total germination of 4 to 6 % compared to the control when pepper seeds were treated with the mycorrhizal formulation. In the second experimental year, an increase in the first count was in the range of 3 to 16 %, whereas an increase in the total germination was 3 to 4 %, compared to the control. The effect of mycorrhizal pepper seed treatment proved beneficial especially to aged seeds that were slow to germinate.
AB  - Cilj istraživanja je bio da se izvrši ocena uticaja godine, populacije semena i tretmana semana formulacijom mikorize na dva najznačajnija pokazatelja kvaliteta semena paprike. Kao materijal u istraživanjima korišćene su tri domaće populacije začinske paprike poreklom sa dva lokaliteta iz Srbije: istočna - Negotin (dve populacije slatka i ljuta) i zapadna - Badovinci (ljuta). Seme populacija paprika proizvedeno je u organskom sistemu gajenja 2017. godine. Utvrđivanje vrednosti pokazatelja kvaliteta (energije klijanja i ukupne klijavosti) semena paprika izvedeno je 2017. i 2018. godine u Laboratoriji za ispitivanje kvaliteta semena poljoprivrednog bilja Instituta za zaštitu bilja i životnu sredinu u Beogradu. Analiza energije klijanja i klijavosti semena paprika pokazala je visoko značajne razlike (r  <0,01) pod uticajem svih pojedinačnih faktora godine (faktor A), populacije semena (faktor B) i tretmana (faktor C). Veoma značajne interakcije ispitivanih faktora u pogledu energije klijanja i ukupne klijavosti semena paprika dobijene su i kod inetrakcije faktora A × B. Tretman semena populacija paprika izveden je komercijalnom formulacijom biostimulatora (Coveron) koji u sastavu sadrži Glomus mosseae, Glomus intraradices i Trihoderma atroviride. Tretmanom semena biostimulatorom u prvoj godini zabeleženo je povećanje energije klijanja i ukupne klijavosti od 4 do 6%, u odnosu na kontrolu. U drugoj godini povećanje energije klijanja kretalo se u interval od 3 do 16%, dok je efekat povećanja ukupne klijavosti bio od 3 do 4%, u odnosu na kontrolu. Porastom starosti semena opada energija klijanja i ukupna klijavost. U tim slučajevima efekat tretmana semena paprika biostimulatorima posebno dobija na značaju, jer utičena poboljšanje parametara kvalieteta semena.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Povećanje kvaliteta semena paprike primenom mikorize
T1  - Increasing the pepper seed quality using mycorrhizal fungi
VL  - 23
IS  - 2
SP  - 66
EP  - 68
DO  - 10.5937/jpea1902066P
UR  - conv_2008
ER  - 
@article{
author = "Poštić, Dobrivoj and Štrbanović, Ratibor and Stanojković-Sebić, Aleksandra and Tabaković, Marijenka and Milivojević, Marija and Jovanović, V. Snežana and Stanisavljević, Rade",
year = "2019",
abstract = "The purpose of this paper is to evaluate the effect of the year of cultivation, seed population and mycorrhizal seed treatment on two most important indicators of the pepper seed quality, namely germination energy and total germination. The pepper seed quality parameters, i.e. the first count and the total germination rate, were examined in the period 2017-2018. The results obtained show significant differences (p   < 0.01) between the parameter values under consideration relative to the year of cultivation (Factor A), seed population (Factor B) and mycorrhizal sees treatment (Factor C). In the first experimental year, there was an increase in the first count and total germination of 4 to 6 % compared to the control when pepper seeds were treated with the mycorrhizal formulation. In the second experimental year, an increase in the first count was in the range of 3 to 16 %, whereas an increase in the total germination was 3 to 4 %, compared to the control. The effect of mycorrhizal pepper seed treatment proved beneficial especially to aged seeds that were slow to germinate., Cilj istraživanja je bio da se izvrši ocena uticaja godine, populacije semena i tretmana semana formulacijom mikorize na dva najznačajnija pokazatelja kvaliteta semena paprike. Kao materijal u istraživanjima korišćene su tri domaće populacije začinske paprike poreklom sa dva lokaliteta iz Srbije: istočna - Negotin (dve populacije slatka i ljuta) i zapadna - Badovinci (ljuta). Seme populacija paprika proizvedeno je u organskom sistemu gajenja 2017. godine. Utvrđivanje vrednosti pokazatelja kvaliteta (energije klijanja i ukupne klijavosti) semena paprika izvedeno je 2017. i 2018. godine u Laboratoriji za ispitivanje kvaliteta semena poljoprivrednog bilja Instituta za zaštitu bilja i životnu sredinu u Beogradu. Analiza energije klijanja i klijavosti semena paprika pokazala je visoko značajne razlike (r  <0,01) pod uticajem svih pojedinačnih faktora godine (faktor A), populacije semena (faktor B) i tretmana (faktor C). Veoma značajne interakcije ispitivanih faktora u pogledu energije klijanja i ukupne klijavosti semena paprika dobijene su i kod inetrakcije faktora A × B. Tretman semena populacija paprika izveden je komercijalnom formulacijom biostimulatora (Coveron) koji u sastavu sadrži Glomus mosseae, Glomus intraradices i Trihoderma atroviride. Tretmanom semena biostimulatorom u prvoj godini zabeleženo je povećanje energije klijanja i ukupne klijavosti od 4 do 6%, u odnosu na kontrolu. U drugoj godini povećanje energije klijanja kretalo se u interval od 3 do 16%, dok je efekat povećanja ukupne klijavosti bio od 3 do 4%, u odnosu na kontrolu. Porastom starosti semena opada energija klijanja i ukupna klijavost. U tim slučajevima efekat tretmana semena paprika biostimulatorima posebno dobija na značaju, jer utičena poboljšanje parametara kvalieteta semena.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Povećanje kvaliteta semena paprike primenom mikorize, Increasing the pepper seed quality using mycorrhizal fungi",
volume = "23",
number = "2",
pages = "66-68",
doi = "10.5937/jpea1902066P",
url = "conv_2008"
}
Poštić, D., Štrbanović, R., Stanojković-Sebić, A., Tabaković, M., Milivojević, M., Jovanović, V. S.,& Stanisavljević, R.. (2019). Povećanje kvaliteta semena paprike primenom mikorize. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 23(2), 66-68.
https://doi.org/10.5937/jpea1902066P
conv_2008
Poštić D, Štrbanović R, Stanojković-Sebić A, Tabaković M, Milivojević M, Jovanović VS, Stanisavljević R. Povećanje kvaliteta semena paprike primenom mikorize. in Journal on Processing and Energy in Agriculture. 2019;23(2):66-68.
doi:10.5937/jpea1902066P
conv_2008 .
Poštić, Dobrivoj, Štrbanović, Ratibor, Stanojković-Sebić, Aleksandra, Tabaković, Marijenka, Milivojević, Marija, Jovanović, V. Snežana, Stanisavljević, Rade, "Povećanje kvaliteta semena paprike primenom mikorize" in Journal on Processing and Energy in Agriculture, 23, no. 2 (2019):66-68,
https://doi.org/10.5937/jpea1902066P .,
conv_2008 .
5

Ispitivanje dormantnosti semena suncokreta

Kojic, Jasna; Milivojević, Marija; Petrović, Tanja; Vukadinović, Radmila

(Beograd : Društvo genetiĉara Srbije, 2018)

TY  - JOUR
AU  - Kojic, Jasna
AU  - Milivojević, Marija
AU  - Petrović, Tanja
AU  - Vukadinović, Radmila
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/971
AB  - U Laboratoriji za ispitivaje semena Instituta za kukuruz Zemun Polje, 
u toku redovnog ispitivanja klijavosti semena suncokreta, uoĉeno je 
kod dve partije semena veliki broj neklijalog semena. Seme je 
stavljeno na naklijavanje dva puta sa razliĉitim temperaturnim 
režimom. U toku prvog ispitivanja seme je stavljeno na klijanje 
metodom između filter papira, hlađenje semena je trajalo 6 dana, 
energija klijanja je oĉitana ĉetvrtog dana a ukupna klijavost desetog 
dana. Seme je naklijavano u klijalištu na 200C sa režmom svetlosti 8 
sati dan, 16 sati noć. U toku drugog ispitivanja, seme suncokreta je 
postavljeno na klijanje istom metodom između filter papira, hlađeno 
je 6 dana, energija klijanja je oĉitana ĉetvrti dan a završna klijavost 
deseti dan. Naklijavanje je obavljeno u sobi klijalištu na naizmeniĉnoj 
temperaturi 20<=>300C, sa svetlosnim režimom 8 sati dan, 16 sati 
noć. Urađen je i TTZ - tetrazolium test. Analizom dobijenih rezultata 
uoĉeno je da na kraju prvog ispitivanja izdvojen veliki broj neklijalog 
semena koje je bilo sveže bez simptoma bolesti. Na kraju drugog 
ispitivanja taj procenat je bio manji u odnosu na prvo ispitivanje. 
Naklijavanje semena na većoj, naizmeniĉnoj temperaturi 20<=>300C, 
u toku drugog ispitivanja, smanjilo je broj neklijalog semena. TTZ 
testom je potvrđena vitalnost neklijalog semena.
AB  - A great number of nongerminated sunflower seed of two lots were 
observed during regular testing in the Seed Testing Laboratory of the 
Maize Research Institute, Zemun Polje. Seeds were germinated 
twice under different temperature regimes. In the course of the first 
test, seeds were germinated between filter paper, cooling lasted 6 
days, the first count was done on the fourth day, while total 
germination was determined on the 10th day. Seeds were germinated 
in the germination cabinet at 200oC with 8 h (light) to16 h (night) 
regime. In the course of the second test, the method applied was the 
same: sunflower seeds were germinated between filter paper, 
cooling lasted 6 days, the first count was done on the fourth day, 
while total germination was determined on the 10th day. Germination 
was performed in the germination cabinet at the alternating 
temperature of 20<=>300oC, and the light regime of 8 h (light) to16 h 
(night). The TTZ -tetrazolium test was also performed. The analysis 
of obtained results showed that a great number of nongermination, 
but fresh and disease-free seeds were detected at the end of the first 
test. The corresponding percentage at the end of the second test 
was smaller. Seed germination at higher, alternating temperatures of 
20<=>300oC, in the course of the second test, reduced the number of 
nongerminated seeds. Seed viability was confirmed by the TTZ test.
PB  - Beograd : Društvo genetiĉara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
T2  - 6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata
T1  - Ispitivanje dormantnosti semena suncokreta
T1  - Evaluation of dormance sunflower seed lots
SP  - 223
EP  - 224
ER  - 
@article{
author = "Kojic, Jasna and Milivojević, Marija and Petrović, Tanja and Vukadinović, Radmila",
year = "2018",
abstract = "U Laboratoriji za ispitivaje semena Instituta za kukuruz Zemun Polje, 
u toku redovnog ispitivanja klijavosti semena suncokreta, uoĉeno je 
kod dve partije semena veliki broj neklijalog semena. Seme je 
stavljeno na naklijavanje dva puta sa razliĉitim temperaturnim 
režimom. U toku prvog ispitivanja seme je stavljeno na klijanje 
metodom između filter papira, hlađenje semena je trajalo 6 dana, 
energija klijanja je oĉitana ĉetvrtog dana a ukupna klijavost desetog 
dana. Seme je naklijavano u klijalištu na 200C sa režmom svetlosti 8 
sati dan, 16 sati noć. U toku drugog ispitivanja, seme suncokreta je 
postavljeno na klijanje istom metodom između filter papira, hlađeno 
je 6 dana, energija klijanja je oĉitana ĉetvrti dan a završna klijavost 
deseti dan. Naklijavanje je obavljeno u sobi klijalištu na naizmeniĉnoj 
temperaturi 20<=>300C, sa svetlosnim režimom 8 sati dan, 16 sati 
noć. Urađen je i TTZ - tetrazolium test. Analizom dobijenih rezultata 
uoĉeno je da na kraju prvog ispitivanja izdvojen veliki broj neklijalog 
semena koje je bilo sveže bez simptoma bolesti. Na kraju drugog 
ispitivanja taj procenat je bio manji u odnosu na prvo ispitivanje. 
Naklijavanje semena na većoj, naizmeniĉnoj temperaturi 20<=>300C, 
u toku drugog ispitivanja, smanjilo je broj neklijalog semena. TTZ 
testom je potvrđena vitalnost neklijalog semena., A great number of nongerminated sunflower seed of two lots were 
observed during regular testing in the Seed Testing Laboratory of the 
Maize Research Institute, Zemun Polje. Seeds were germinated 
twice under different temperature regimes. In the course of the first 
test, seeds were germinated between filter paper, cooling lasted 6 
days, the first count was done on the fourth day, while total 
germination was determined on the 10th day. Seeds were germinated 
in the germination cabinet at 200oC with 8 h (light) to16 h (night) 
regime. In the course of the second test, the method applied was the 
same: sunflower seeds were germinated between filter paper, 
cooling lasted 6 days, the first count was done on the fourth day, 
while total germination was determined on the 10th day. Germination 
was performed in the germination cabinet at the alternating 
temperature of 20<=>300oC, and the light regime of 8 h (light) to16 h 
(night). The TTZ -tetrazolium test was also performed. The analysis 
of obtained results showed that a great number of nongermination, 
but fresh and disease-free seeds were detected at the end of the first 
test. The corresponding percentage at the end of the second test 
was smaller. Seed germination at higher, alternating temperatures of 
20<=>300oC, in the course of the second test, reduced the number of 
nongerminated seeds. Seed viability was confirmed by the TTZ test.",
publisher = "Beograd : Društvo genetiĉara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata",
title = "Ispitivanje dormantnosti semena suncokreta, Evaluation of dormance sunflower seed lots",
pages = "223-224"
}
Kojic, J., Milivojević, M., Petrović, T.,& Vukadinović, R.. (2018). Ispitivanje dormantnosti semena suncokreta. in 6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata
Beograd : Društvo genetiĉara Srbije., 223-224.
Kojic J, Milivojević M, Petrović T, Vukadinović R. Ispitivanje dormantnosti semena suncokreta. in 6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata. 2018;:223-224..
Kojic, Jasna, Milivojević, Marija, Petrović, Tanja, Vukadinović, Radmila, "Ispitivanje dormantnosti semena suncokreta" in 6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata (2018):223-224.

ISTA rules changes in seed germination testing at the beginning of the 21st century

Milivojević, Marija; Ripka, Zita; Petrović, Tanja

(Novi Sad : National Society of Processing and Energy in Agriculture, 2018)

TY  - JOUR
AU  - Milivojević, Marija
AU  - Ripka, Zita
AU  - Petrović, Tanja
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/787
AB  - The International Seed Testing Association (ISTA) was established in 1924 with the idea of standardization in seed testing worldwide. The first international rules for seed testing (ISTA Rules) were published in 1931. The development of ISTA Rules is constant. ISTA members are involved in the ongoing process of developing methods for seed sampling and testing. The methods are validated to ensure that test procedures provide reliable and reproducible results. Since 2001, ISTA Rules have been issued annually. ISTA accredited laboratories have to work according to valid ISTA Rules. In this paper, ISTA Rules changes in seed germination testing since 2001 were presented. Changes in testing methods, criteria for seedling evaluation, procedure of calculation and reporting of results are presented in detail. It can be concluded that the amendments to the ISTA Rules are necessary in order to harmonize seed testing and modern trends in seed trade.
AB  - Među   narodna organizacija za ispitivanje semena (ISTA) je osnovana 1924. godine sa idejom ujednačavanja procesa ispitivanja semena širom sveta. Prva međunarodna pravila za ispitivanje semena (ISTA Pravila) su objavljena 1931. godine. Razvoj ISTA Pravila je konstantan. ISTA članice su uključene u stalan proces razvoja metoda za uzorkovanje i ispitivanje semena. Metode prolaze kroz odgovarajuće ispitivanje validnosti kako bi se obezbedilo da procedure testova daju pouzdane i ponovljive rezultate. Od 2001. godine izdaju se nova ISTA Pravila svake godine. ISTA akreditovane laboratorije imaju obavezu da svoj sistem kvaliteta i svoj rad usaglase sa važećim ISTA Pravilima. U ovom radu prikazane su izmene ISTA Pravila u oblasti ispitivanja klijavosti semena od 2001. godine kao najvažnijeg parametra kvaliteta za promet semena. Pregled izmena ISTA Pravila je sproveden korišćenjem štampanih i elektronskih  izdanja.  U  analizi  su  takođe  korišćeni  dokumenti  sa  ISTA  godišnjih  sastanaka  u  kojima  su  data  objašnjenja  za predložene  izmene.  Analizom  je  ustanovljeno  da  su  u  prethodnih  18  godina  uvedene  brojne  izmene  u  ISTA  Pravila  za  oblast  ispitivanja  klijavosti.  Metode  klijavosti  za  trinaest  novih  vrsta  je  uvedeno  u  Pravila,  nekoliko  metoda  je  izmenjeno,  dozvoljena  je  upotreba  novih  substrata za naklijavanje kao i kombinacija substrata. Novi tipovi nenormalnosti su dodati i za određene vrste su izmenjeni kriterijumi za ocenu klijavosti. Za veliki broj pravila su data dodatna objašnjenja. Najvažnije izmene pravila su doprinele da  procesispitivanja  klijavosti  semena  postane  brži  i  jednostavniji  što  je  neophodno  radi  usklađivanja  načina  ispitivanja  i savremenih tokova u prometu semena.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
T2  - Journal on processing and energy in agriculture
T1  - ISTA rules changes in seed germination testing at the beginning of the 21st century
T1  - Izmene ISTA pravila za ispitivanje klijavosti semena na početku 21. veka
VL  - 22
IS  - 1
SP  - 40
EP  - 45
DO  - 10.5937/JPEA1801040M
ER  - 
@article{
author = "Milivojević, Marija and Ripka, Zita and Petrović, Tanja",
year = "2018",
abstract = "The International Seed Testing Association (ISTA) was established in 1924 with the idea of standardization in seed testing worldwide. The first international rules for seed testing (ISTA Rules) were published in 1931. The development of ISTA Rules is constant. ISTA members are involved in the ongoing process of developing methods for seed sampling and testing. The methods are validated to ensure that test procedures provide reliable and reproducible results. Since 2001, ISTA Rules have been issued annually. ISTA accredited laboratories have to work according to valid ISTA Rules. In this paper, ISTA Rules changes in seed germination testing since 2001 were presented. Changes in testing methods, criteria for seedling evaluation, procedure of calculation and reporting of results are presented in detail. It can be concluded that the amendments to the ISTA Rules are necessary in order to harmonize seed testing and modern trends in seed trade., Među   narodna organizacija za ispitivanje semena (ISTA) je osnovana 1924. godine sa idejom ujednačavanja procesa ispitivanja semena širom sveta. Prva međunarodna pravila za ispitivanje semena (ISTA Pravila) su objavljena 1931. godine. Razvoj ISTA Pravila je konstantan. ISTA članice su uključene u stalan proces razvoja metoda za uzorkovanje i ispitivanje semena. Metode prolaze kroz odgovarajuće ispitivanje validnosti kako bi se obezbedilo da procedure testova daju pouzdane i ponovljive rezultate. Od 2001. godine izdaju se nova ISTA Pravila svake godine. ISTA akreditovane laboratorije imaju obavezu da svoj sistem kvaliteta i svoj rad usaglase sa važećim ISTA Pravilima. U ovom radu prikazane su izmene ISTA Pravila u oblasti ispitivanja klijavosti semena od 2001. godine kao najvažnijeg parametra kvaliteta za promet semena. Pregled izmena ISTA Pravila je sproveden korišćenjem štampanih i elektronskih  izdanja.  U  analizi  su  takođe  korišćeni  dokumenti  sa  ISTA  godišnjih  sastanaka  u  kojima  su  data  objašnjenja  za predložene  izmene.  Analizom  je  ustanovljeno  da  su  u  prethodnih  18  godina  uvedene  brojne  izmene  u  ISTA  Pravila  za  oblast  ispitivanja  klijavosti.  Metode  klijavosti  za  trinaest  novih  vrsta  je  uvedeno  u  Pravila,  nekoliko  metoda  je  izmenjeno,  dozvoljena  je  upotreba  novih  substrata za naklijavanje kao i kombinacija substrata. Novi tipovi nenormalnosti su dodati i za određene vrste su izmenjeni kriterijumi za ocenu klijavosti. Za veliki broj pravila su data dodatna objašnjenja. Najvažnije izmene pravila su doprinele da  procesispitivanja  klijavosti  semena  postane  brži  i  jednostavniji  što  je  neophodno  radi  usklađivanja  načina  ispitivanja  i savremenih tokova u prometu semena.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture",
journal = "Journal on processing and energy in agriculture",
title = "ISTA rules changes in seed germination testing at the beginning of the 21st century, Izmene ISTA pravila za ispitivanje klijavosti semena na početku 21. veka",
volume = "22",
number = "1",
pages = "40-45",
doi = "10.5937/JPEA1801040M"
}
Milivojević, M., Ripka, Z.,& Petrović, T.. (2018). ISTA rules changes in seed germination testing at the beginning of the 21st century. in Journal on processing and energy in agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 22(1), 40-45.
https://doi.org/10.5937/JPEA1801040M
Milivojević M, Ripka Z, Petrović T. ISTA rules changes in seed germination testing at the beginning of the 21st century. in Journal on processing and energy in agriculture. 2018;22(1):40-45.
doi:10.5937/JPEA1801040M .
Milivojević, Marija, Ripka, Zita, Petrović, Tanja, "ISTA rules changes in seed germination testing at the beginning of the 21st century" in Journal on processing and energy in agriculture, 22, no. 1 (2018):40-45,
https://doi.org/10.5937/JPEA1801040M . .
8

Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2

Milivojević, Marija; Kojić, Jasna; Vukadinović, Radmila; Stanisavljević, Rade; Poštić, Dobrivoj; Petrović, Tanja

(Beograd : Društvo selekcionara i semenara Republike Srbije, 2018)

TY  - JOUR
AU  - Milivojević, Marija
AU  - Kojić, Jasna
AU  - Vukadinović, Radmila
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Petrović, Tanja
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/784
AB  - Pojava tvrdog semena kao posledica nepropustljivosti semenjače za vodu je izražena kod vrsta iz familije Fabaceae. Kod divljih biljaka ova osobina je biološki opravdana jer omoguća-va bolji opstanak, ali za poljoprivredu tvrda semena su nepoželjna jer klijaju sa zakašnjenjem i neravnomerno. Prilikom ispitivanja klijavosti semena ovih vrsta u laboratoriji može se primeniti predtretman (prethodno hlađenje, mehanička skarifikacija) pre naklijavanja u optimalnim uslovi-ma. U ovom radu su prikazani rezultati ispitivanja klijavosti semena Trifolium repens sprovedena u 150 laboratorija širom sveta u okviru Proficiency testa 17-2. Ovi testovi su organizovani od strane Međunarodne organizacije za ispitivanje semena (ISTA), a obavezni su za sve akredi-tovane laboratorije, jer se na taj način kontroliše kompetentnost osoblja, ispravnost aparata i pou-zdanost primenjenih metoda ispitivanja. U avgustu 2017. godine distribuirani su uzorci tri partije semena Trifolium repens. U Laboratoriji za ispitivanje semena u Institutu za kukuruz Zemun Polje (LIZP), ispitana je klijavost u septembru primenom mehaničke skarifikacije i prethodnog hlađenja (2 dana na 5-10°C), a zatim klijanje na 20°C. Od 90 akreditovanih laboratorija samo je još jedna primenila mehaničku skarifikaciju, 42 laboratorije nisu primenile nikakav predtretman, a 39 laboratorija je primenilo prethodno hlađenje. Dobijeni rezultati klijavosti u LIZP (Lot1-84.50%, Lot2-84%, Lot3-88.50%) su bili iznad proseka svih laboratorija (Lot1-76.95%, Lot2-79.63%, Lot3-83.27%). Kao glavni zaključak ovog Proficiency testa jeste da je skarifikacija se-mena značajno smanjila broj tvrdih semena u korist normalnih klijanaca. Razlog retke primene skarifikacije može biti potreba korisnika da dobije adekvatnu informaciju vezanu za broj tvrdih semena koja će se pojaviti prilikom setve semena.
AB  - The  presence  of  hard  seeds  as  a  result  of  water  impermeable  seed  coat  is  common  in  species  of  Fabaceae  family.  For  wild  plants,  this  characteristic  allows  better  survival,  but  for  agriculture  production,  hard  seeds  are  undesirable,  due  to  delayed  and  uneven  germination.  In  seed testing laboratory several pre-treatments (pre-chill, mechanical scarification) can be applied for germination testing of these species. In this paper, Proficiency test 17-2 germination results of Trifolium repens seeds conducted in 150 laboratories around the world are presented. Those tests are organized by the International Seed Testing Association (ISTA) and are mandatory for all accredited laboratories. In this way laboratory’s competence (staff, apparatus, test methods) is checked. In August 2017, samples of three Trifolium  repens  seed  lots  were  distributed.  In  the Seed Testing Laboratory at the Maize Research Institute Zemun Polje (LIZP), germination was tested in September using mechanical scarification and pre-chilling (2 days at 5-10°C) prior to germination at 20°C. Among 90 accredited participants, only one more laboratory applied mechanical scarification, 42 laboratories did not use any pre-treatment, and 39 laboratories applied pre-chilling. Obtained germination results in LIZP (Lot1-84.50%, Lot2-84%, Lot3-88.50%) were higher than overall mean (Lot1-76.95%, Lot2-79.63%, and Lot3-83.27%). The main conclusion of this Proficiency test is that seed scarification significantly reduced the number of hard seeds in favour of normal seedlings. The reason for the rare application of scarification may be the requirement of the applicant to obtain adequate information regarding the number of hard seeds that will appear during seed sowing.
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2
T1  - ISTA proficiency test 17-2 germination results of white clover seed
VL  - 24
IS  - 1
SP  - 11
EP  - 17
DO  - 10.5937/SelSem1801011M
ER  - 
@article{
author = "Milivojević, Marija and Kojić, Jasna and Vukadinović, Radmila and Stanisavljević, Rade and Poštić, Dobrivoj and Petrović, Tanja",
year = "2018",
abstract = "Pojava tvrdog semena kao posledica nepropustljivosti semenjače za vodu je izražena kod vrsta iz familije Fabaceae. Kod divljih biljaka ova osobina je biološki opravdana jer omoguća-va bolji opstanak, ali za poljoprivredu tvrda semena su nepoželjna jer klijaju sa zakašnjenjem i neravnomerno. Prilikom ispitivanja klijavosti semena ovih vrsta u laboratoriji može se primeniti predtretman (prethodno hlađenje, mehanička skarifikacija) pre naklijavanja u optimalnim uslovi-ma. U ovom radu su prikazani rezultati ispitivanja klijavosti semena Trifolium repens sprovedena u 150 laboratorija širom sveta u okviru Proficiency testa 17-2. Ovi testovi su organizovani od strane Međunarodne organizacije za ispitivanje semena (ISTA), a obavezni su za sve akredi-tovane laboratorije, jer se na taj način kontroliše kompetentnost osoblja, ispravnost aparata i pou-zdanost primenjenih metoda ispitivanja. U avgustu 2017. godine distribuirani su uzorci tri partije semena Trifolium repens. U Laboratoriji za ispitivanje semena u Institutu za kukuruz Zemun Polje (LIZP), ispitana je klijavost u septembru primenom mehaničke skarifikacije i prethodnog hlađenja (2 dana na 5-10°C), a zatim klijanje na 20°C. Od 90 akreditovanih laboratorija samo je još jedna primenila mehaničku skarifikaciju, 42 laboratorije nisu primenile nikakav predtretman, a 39 laboratorija je primenilo prethodno hlađenje. Dobijeni rezultati klijavosti u LIZP (Lot1-84.50%, Lot2-84%, Lot3-88.50%) su bili iznad proseka svih laboratorija (Lot1-76.95%, Lot2-79.63%, Lot3-83.27%). Kao glavni zaključak ovog Proficiency testa jeste da je skarifikacija se-mena značajno smanjila broj tvrdih semena u korist normalnih klijanaca. Razlog retke primene skarifikacije može biti potreba korisnika da dobije adekvatnu informaciju vezanu za broj tvrdih semena koja će se pojaviti prilikom setve semena., The  presence  of  hard  seeds  as  a  result  of  water  impermeable  seed  coat  is  common  in  species  of  Fabaceae  family.  For  wild  plants,  this  characteristic  allows  better  survival,  but  for  agriculture  production,  hard  seeds  are  undesirable,  due  to  delayed  and  uneven  germination.  In  seed testing laboratory several pre-treatments (pre-chill, mechanical scarification) can be applied for germination testing of these species. In this paper, Proficiency test 17-2 germination results of Trifolium repens seeds conducted in 150 laboratories around the world are presented. Those tests are organized by the International Seed Testing Association (ISTA) and are mandatory for all accredited laboratories. In this way laboratory’s competence (staff, apparatus, test methods) is checked. In August 2017, samples of three Trifolium  repens  seed  lots  were  distributed.  In  the Seed Testing Laboratory at the Maize Research Institute Zemun Polje (LIZP), germination was tested in September using mechanical scarification and pre-chilling (2 days at 5-10°C) prior to germination at 20°C. Among 90 accredited participants, only one more laboratory applied mechanical scarification, 42 laboratories did not use any pre-treatment, and 39 laboratories applied pre-chilling. Obtained germination results in LIZP (Lot1-84.50%, Lot2-84%, Lot3-88.50%) were higher than overall mean (Lot1-76.95%, Lot2-79.63%, and Lot3-83.27%). The main conclusion of this Proficiency test is that seed scarification significantly reduced the number of hard seeds in favour of normal seedlings. The reason for the rare application of scarification may be the requirement of the applicant to obtain adequate information regarding the number of hard seeds that will appear during seed sowing.",
publisher = "Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2, ISTA proficiency test 17-2 germination results of white clover seed",
volume = "24",
number = "1",
pages = "11-17",
doi = "10.5937/SelSem1801011M"
}
Milivojević, M., Kojić, J., Vukadinović, R., Stanisavljević, R., Poštić, D.,& Petrović, T.. (2018). Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2. in Selekcija i semenarstvo
Beograd : Društvo selekcionara i semenara Republike Srbije., 24(1), 11-17.
https://doi.org/10.5937/SelSem1801011M
Milivojević M, Kojić J, Vukadinović R, Stanisavljević R, Poštić D, Petrović T. Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2. in Selekcija i semenarstvo. 2018;24(1):11-17.
doi:10.5937/SelSem1801011M .
Milivojević, Marija, Kojić, Jasna, Vukadinović, Radmila, Stanisavljević, Rade, Poštić, Dobrivoj, Petrović, Tanja, "Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2" in Selekcija i semenarstvo, 24, no. 1 (2018):11-17,
https://doi.org/10.5937/SelSem1801011M . .

Disruption of genetic identity for genebank maize accessions during conservation

Babić, Vojka; Anđelković, Violeta; Nikolić, Ana; Milivojević, Marija; Srdić, Jelena; Popović, Aleksandar; Kravić, Natalija

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Babić, Vojka
AU  - Anđelković, Violeta
AU  - Nikolić, Ana
AU  - Milivojević, Marija
AU  - Srdić, Jelena
AU  - Popović, Aleksandar
AU  - Kravić, Natalija
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/658
AB  - Maintenance of the original accessions identity and integrity is one of the priorities among genebank activities. Different factors related to conservation may result in accessions disruption. Regeneration is the most frequent critical point in this process, due to bottlenecks, inbreeding, random genetic drift and unintentional mixing or contamination. On the other hand, genetic drift may occur due to seed viability loss. Therefore, it is very important to establish the balance between the frequency of regeneration and the duration of accession conservation. The aim of the present study was to estimate whether the identity of accessions regenerated after 27 years of medium-term conservation was disrupted. Phenotypic markers were applied on three Maize Research Institute "Zemun Polje" (MRIZP) genebank maize landraces (K2026, K768 and K86), differing in seed viability, kernel type and effective population size. It was estimated that, after the regeneration, there had been no significant changes in the landrace K2026. There were some parameters indicating that genetic drift had occurred in the landrace K768, and that there had been even a certain degree of inbreeding in the landrace K86. According to the results, accession K2026 could still be kept under the same ID number. Due to the genuine identity disruption, assignment of new ID numbers for K768 and K86 should be suggested.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Disruption of genetic identity for genebank maize accessions during conservation
VL  - 49
IS  - 3
SP  - 853
EP  - 864
DO  - 10.2298/GENSR1703853B
UR  - conv_969
ER  - 
@article{
author = "Babić, Vojka and Anđelković, Violeta and Nikolić, Ana and Milivojević, Marija and Srdić, Jelena and Popović, Aleksandar and Kravić, Natalija",
year = "2017",
abstract = "Maintenance of the original accessions identity and integrity is one of the priorities among genebank activities. Different factors related to conservation may result in accessions disruption. Regeneration is the most frequent critical point in this process, due to bottlenecks, inbreeding, random genetic drift and unintentional mixing or contamination. On the other hand, genetic drift may occur due to seed viability loss. Therefore, it is very important to establish the balance between the frequency of regeneration and the duration of accession conservation. The aim of the present study was to estimate whether the identity of accessions regenerated after 27 years of medium-term conservation was disrupted. Phenotypic markers were applied on three Maize Research Institute "Zemun Polje" (MRIZP) genebank maize landraces (K2026, K768 and K86), differing in seed viability, kernel type and effective population size. It was estimated that, after the regeneration, there had been no significant changes in the landrace K2026. There were some parameters indicating that genetic drift had occurred in the landrace K768, and that there had been even a certain degree of inbreeding in the landrace K86. According to the results, accession K2026 could still be kept under the same ID number. Due to the genuine identity disruption, assignment of new ID numbers for K768 and K86 should be suggested.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Disruption of genetic identity for genebank maize accessions during conservation",
volume = "49",
number = "3",
pages = "853-864",
doi = "10.2298/GENSR1703853B",
url = "conv_969"
}
Babić, V., Anđelković, V., Nikolić, A., Milivojević, M., Srdić, J., Popović, A.,& Kravić, N.. (2017). Disruption of genetic identity for genebank maize accessions during conservation. in Genetika
Društvo genetičara Srbije, Beograd., 49(3), 853-864.
https://doi.org/10.2298/GENSR1703853B
conv_969
Babić V, Anđelković V, Nikolić A, Milivojević M, Srdić J, Popović A, Kravić N. Disruption of genetic identity for genebank maize accessions during conservation. in Genetika. 2017;49(3):853-864.
doi:10.2298/GENSR1703853B
conv_969 .
Babić, Vojka, Anđelković, Violeta, Nikolić, Ana, Milivojević, Marija, Srdić, Jelena, Popović, Aleksandar, Kravić, Natalija, "Disruption of genetic identity for genebank maize accessions during conservation" in Genetika, 49, no. 3 (2017):853-864,
https://doi.org/10.2298/GENSR1703853B .,
conv_969 .

Testing of maize inbreed lines seed germination in the soil

Kojić, Jasna; Milivojević, Marija; Petrović, Tanja; Tabaković, Marijenka; Stevanović, Nikola

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, 2017)

TY  - CONF
AU  - Kojić, Jasna
AU  - Milivojević, Marija
AU  - Petrović, Tanja
AU  - Tabaković, Marijenka
AU  - Stevanović, Nikola
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/889
AB  - The aim of this paper is to present soil as a growing medium used in germination tests of
seeds of some insecticide-treated maize inbred lines that have been carried out at the seed
testing laboratory of the Maize Research Institute, Zemun Polje in Belgrade. Although soil is
not recommended as a primary growing medium for seed germination because it is not
included into standard methods, it is the most natural and the best growing medium for seed
germination testing under laboratory conditions. Soil is recommended when seedlings show
phytotoxic symptoms or when results of the standard germination test are uncertain. The
standard method B(etween) P(aper) with 4x100 seeds and filter paper + soil method with
4x50 seeds were applied in the study in 2015 and 2016. Seeds were treated with the fungicide
Maxim Xl and the insecticide Sonido. Soil used in the study was degraded chernozem taken
from a maize field and prepared for the medium according to the standard procedure. Seeds
were germinated in the germination room at the temperature of 20<=>30oC and 16:8 of light :
dark photoperiod. The first count and total germination were recorded on the 4th and the 7th
day, respectively, in both methods. Obtained results indicate that total germination for all
maize inbred lines was greater in both years of investigation when filter paper + soil method
was applied.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture
C3  - VIII International scientific agricultural symposium „Agrosym 2017“ of Republic of Srpska, October 05-08, 2017. Jahorina, Bosnia and Herzegovina - Book of Proceedings
T1  - Testing of maize inbreed lines seed germination in the soil
SP  - 525
EP  - 529
ER  - 
@conference{
author = "Kojić, Jasna and Milivojević, Marija and Petrović, Tanja and Tabaković, Marijenka and Stevanović, Nikola",
year = "2017",
abstract = "The aim of this paper is to present soil as a growing medium used in germination tests of
seeds of some insecticide-treated maize inbred lines that have been carried out at the seed
testing laboratory of the Maize Research Institute, Zemun Polje in Belgrade. Although soil is
not recommended as a primary growing medium for seed germination because it is not
included into standard methods, it is the most natural and the best growing medium for seed
germination testing under laboratory conditions. Soil is recommended when seedlings show
phytotoxic symptoms or when results of the standard germination test are uncertain. The
standard method B(etween) P(aper) with 4x100 seeds and filter paper + soil method with
4x50 seeds were applied in the study in 2015 and 2016. Seeds were treated with the fungicide
Maxim Xl and the insecticide Sonido. Soil used in the study was degraded chernozem taken
from a maize field and prepared for the medium according to the standard procedure. Seeds
were germinated in the germination room at the temperature of 20<=>30oC and 16:8 of light :
dark photoperiod. The first count and total germination were recorded on the 4th and the 7th
day, respectively, in both methods. Obtained results indicate that total germination for all
maize inbred lines was greater in both years of investigation when filter paper + soil method
was applied.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture",
journal = "VIII International scientific agricultural symposium „Agrosym 2017“ of Republic of Srpska, October 05-08, 2017. Jahorina, Bosnia and Herzegovina - Book of Proceedings",
title = "Testing of maize inbreed lines seed germination in the soil",
pages = "525-529"
}
Kojić, J., Milivojević, M., Petrović, T., Tabaković, M.,& Stevanović, N.. (2017). Testing of maize inbreed lines seed germination in the soil. in VIII International scientific agricultural symposium „Agrosym 2017“ of Republic of Srpska, October 05-08, 2017. Jahorina, Bosnia and Herzegovina - Book of Proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture., 525-529.
Kojić J, Milivojević M, Petrović T, Tabaković M, Stevanović N. Testing of maize inbreed lines seed germination in the soil. in VIII International scientific agricultural symposium „Agrosym 2017“ of Republic of Srpska, October 05-08, 2017. Jahorina, Bosnia and Herzegovina - Book of Proceedings. 2017;:525-529..
Kojić, Jasna, Milivojević, Marija, Petrović, Tanja, Tabaković, Marijenka, Stevanović, Nikola, "Testing of maize inbreed lines seed germination in the soil" in VIII International scientific agricultural symposium „Agrosym 2017“ of Republic of Srpska, October 05-08, 2017. Jahorina, Bosnia and Herzegovina - Book of Proceedings (2017):525-529.

Relationship between genetic diversity and cold-tolerance of maize inbred lines

Milivojević, Marija; Nikolić, Ana; Marković, Ksenija; Filipović, Milomir; Vančetović, Jelena; Petrović, Tanja; Srdić, Jelena

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Milivojević, Marija
AU  - Nikolić, Ana
AU  - Marković, Ksenija
AU  - Filipović, Milomir
AU  - Vančetović, Jelena
AU  - Petrović, Tanja
AU  - Srdić, Jelena
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/692
AB  - Early maize sowing enables longer growing season with enhanced possibility of achieving higher and more stable yields, and better chances of avoiding summer droughts. For early sowing, cold-tolerant maize genotypes should be used. Breeding maize, tolerant to low temperatures, requires knowledge of genetic diversity and heterotic patterns of breeding material. The objective of this study was to determine genetic diversity of 15 ZP maize inbred lines applying the method of protein markers (UTLIEF method), and to establish correspondence between thus obtained classification with the results of cold test (CT) and field emergence (FE). During two production seasons (2011 and 2014), 15 maize inbred lines were self-pollinated. Pedigree data showed that material belongs to different maturity and heterotic groups. Cold tolerance was assessed in laboratory (2015) by cold test (7.5 degrees C, 10 days), and field trials on two locations during two successive years (2015, 2016). ZP maize inbred lines showed very good response to stressful conditions of CT and FE. Inbred lines with Lancaster background were more sensitive to low temperatures than inbred lines with BSSS and Iowa Dent background. Based on UTLIEF method two inbred lines with Lancaster background (ZPL 5 and ZPL 7) were grouped by cluster analysis together with Iowa Dent inbred lines, that also expressed better cold tolerance, and thus exceptional consent was achieved with the results of CT and FE. Classification of maize inbred lines based on UTLIEF method, followed by cluster analysis and PCA, showed good agreement with pedigree data, which points out that this method could be successfully applied for genetic classification of breeding material of a wide genetic background.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Relationship between genetic diversity and cold-tolerance of maize inbred lines
VL  - 49
IS  - 2
SP  - 635
EP  - 646
DO  - 10.2298/GENSR1702635M
UR  - conv_964
ER  - 
@article{
author = "Milivojević, Marija and Nikolić, Ana and Marković, Ksenija and Filipović, Milomir and Vančetović, Jelena and Petrović, Tanja and Srdić, Jelena",
year = "2017",
abstract = "Early maize sowing enables longer growing season with enhanced possibility of achieving higher and more stable yields, and better chances of avoiding summer droughts. For early sowing, cold-tolerant maize genotypes should be used. Breeding maize, tolerant to low temperatures, requires knowledge of genetic diversity and heterotic patterns of breeding material. The objective of this study was to determine genetic diversity of 15 ZP maize inbred lines applying the method of protein markers (UTLIEF method), and to establish correspondence between thus obtained classification with the results of cold test (CT) and field emergence (FE). During two production seasons (2011 and 2014), 15 maize inbred lines were self-pollinated. Pedigree data showed that material belongs to different maturity and heterotic groups. Cold tolerance was assessed in laboratory (2015) by cold test (7.5 degrees C, 10 days), and field trials on two locations during two successive years (2015, 2016). ZP maize inbred lines showed very good response to stressful conditions of CT and FE. Inbred lines with Lancaster background were more sensitive to low temperatures than inbred lines with BSSS and Iowa Dent background. Based on UTLIEF method two inbred lines with Lancaster background (ZPL 5 and ZPL 7) were grouped by cluster analysis together with Iowa Dent inbred lines, that also expressed better cold tolerance, and thus exceptional consent was achieved with the results of CT and FE. Classification of maize inbred lines based on UTLIEF method, followed by cluster analysis and PCA, showed good agreement with pedigree data, which points out that this method could be successfully applied for genetic classification of breeding material of a wide genetic background.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Relationship between genetic diversity and cold-tolerance of maize inbred lines",
volume = "49",
number = "2",
pages = "635-646",
doi = "10.2298/GENSR1702635M",
url = "conv_964"
}
Milivojević, M., Nikolić, A., Marković, K., Filipović, M., Vančetović, J., Petrović, T.,& Srdić, J.. (2017). Relationship between genetic diversity and cold-tolerance of maize inbred lines. in Genetika
Društvo genetičara Srbije, Beograd., 49(2), 635-646.
https://doi.org/10.2298/GENSR1702635M
conv_964
Milivojević M, Nikolić A, Marković K, Filipović M, Vančetović J, Petrović T, Srdić J. Relationship between genetic diversity and cold-tolerance of maize inbred lines. in Genetika. 2017;49(2):635-646.
doi:10.2298/GENSR1702635M
conv_964 .
Milivojević, Marija, Nikolić, Ana, Marković, Ksenija, Filipović, Milomir, Vančetović, Jelena, Petrović, Tanja, Srdić, Jelena, "Relationship between genetic diversity and cold-tolerance of maize inbred lines" in Genetika, 49, no. 2 (2017):635-646,
https://doi.org/10.2298/GENSR1702635M .,
conv_964 .
4
2
2

Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines

Milivojević, Marija; Srdić, Jelena; Branković-Radojčić, Dragana V.; Vukadinović, Radmila; Kojić, Jasna; Petrović, Tanja

(Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad, 2017)

TY  - JOUR
AU  - Milivojević, Marija
AU  - Srdić, Jelena
AU  - Branković-Radojčić, Dragana V.
AU  - Vukadinović, Radmila
AU  - Kojić, Jasna
AU  - Petrović, Tanja
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/672
AB  - Cold test (CT) is a valuable method of assessing maize seed vigour. Although widely used CT is not standardized because of variations in CT procedures. The objective of this study was to evaluate seed vigour of 15 maize inbred lines developed at the Maize Research Institute Zemun Polje, using two different substrates (soil and sand) in a severe CT (7.5°C for 10 days followed by 7 days at 20/30 °C). Statistical analysis showed that inbred lines differ significantly in seed vigour, and no difference between two applied substrates in CT. Coefficients of variation were lower in CT with sand (6.05 %), compared to the CT with soil (6.74%) due to variation in soil quality and presence of soil-borne pathogens. Both CT procedures were highly correlated with field emergence. Results of this research indicate that CT with sand is appropriate for testing maize seed vigour, with the potential for standardization.
AB  - Vigor semena je veoma važan parametar kvaliteta, jer ukazuje na sposobnost semena da klija u suboptimalnim uslovima (niska temperatura, visoka vlažnost zemljišta itd.). Hladni test je važan metod za utvrđivanje vigora semena kukuruza. Iako je dobar pokazatelj nicanja u polju, i koristi se širom sveta, hladni test nije standardizovan zbog razlika u proceduri (temperatura, vrsta i vlažnost supstrata). Cilj ovog rada bio je ispitivanje vigora semena samooplodnih linija kukuruza primenom dva različita supstrata (zemlja i pesak) u hladnom testu. Za ispitivanje je odabrano 15 samooplodnih linija kukuruza stvorenih u Institutu za kukuruz 'Zemun Polje'. Primenjeni su izrazito stresni uslovi ispitivanja u hladnom testu (10 dana na 7,5°C, a zatim 7 dana na 20/30°C). Statističkom obradom podataka utvrđeno je da su se samooplodne linije značajno razlikovale po vigoru semena tj. tolerantnosti na niske temperature u periodu klijanja i početnog porasta. Sa druge strane nije utvrđena značajna razlika između dva primenjena supstrata u hladnom testu. Prosečna klijavost 15 samooplodnih linija u hladnom testu sa peskom iznosila je 82,2%, a u hladnom testu sa zemljom 80,1%. Koeficijent varijacije je bio niži u hladnom testu sa peskom (6,05%), nego u hladnom testu sa zemljom (6,74%), što se objašnjava variranjem u kvalitetu zemlje i prisustvu zemljišnih patogena. Obe ispitivane procedure su postigle visoku korelaciju sa nicanjem u polju. Rezultati ovih istraživanja ukazuju na mogućnost korišćenja peska kao supstrata u hladnom testu, čime bi se ispitivanje vigora semena kukuruza znatno olakšalo, a ujedno bi se stekli uslovi za standardizaciju hladnog testa.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines
T1  - Poređenje dve procedure hladnog testa za ispitivanje vigora semena samooplodnih linija kukuruza
VL  - 21
IS  - 2
SP  - 115
EP  - 117
DO  - 10.5937/JPEA1702115M
UR  - conv_647
ER  - 
@article{
author = "Milivojević, Marija and Srdić, Jelena and Branković-Radojčić, Dragana V. and Vukadinović, Radmila and Kojić, Jasna and Petrović, Tanja",
year = "2017",
abstract = "Cold test (CT) is a valuable method of assessing maize seed vigour. Although widely used CT is not standardized because of variations in CT procedures. The objective of this study was to evaluate seed vigour of 15 maize inbred lines developed at the Maize Research Institute Zemun Polje, using two different substrates (soil and sand) in a severe CT (7.5°C for 10 days followed by 7 days at 20/30 °C). Statistical analysis showed that inbred lines differ significantly in seed vigour, and no difference between two applied substrates in CT. Coefficients of variation were lower in CT with sand (6.05 %), compared to the CT with soil (6.74%) due to variation in soil quality and presence of soil-borne pathogens. Both CT procedures were highly correlated with field emergence. Results of this research indicate that CT with sand is appropriate for testing maize seed vigour, with the potential for standardization., Vigor semena je veoma važan parametar kvaliteta, jer ukazuje na sposobnost semena da klija u suboptimalnim uslovima (niska temperatura, visoka vlažnost zemljišta itd.). Hladni test je važan metod za utvrđivanje vigora semena kukuruza. Iako je dobar pokazatelj nicanja u polju, i koristi se širom sveta, hladni test nije standardizovan zbog razlika u proceduri (temperatura, vrsta i vlažnost supstrata). Cilj ovog rada bio je ispitivanje vigora semena samooplodnih linija kukuruza primenom dva različita supstrata (zemlja i pesak) u hladnom testu. Za ispitivanje je odabrano 15 samooplodnih linija kukuruza stvorenih u Institutu za kukuruz 'Zemun Polje'. Primenjeni su izrazito stresni uslovi ispitivanja u hladnom testu (10 dana na 7,5°C, a zatim 7 dana na 20/30°C). Statističkom obradom podataka utvrđeno je da su se samooplodne linije značajno razlikovale po vigoru semena tj. tolerantnosti na niske temperature u periodu klijanja i početnog porasta. Sa druge strane nije utvrđena značajna razlika između dva primenjena supstrata u hladnom testu. Prosečna klijavost 15 samooplodnih linija u hladnom testu sa peskom iznosila je 82,2%, a u hladnom testu sa zemljom 80,1%. Koeficijent varijacije je bio niži u hladnom testu sa peskom (6,05%), nego u hladnom testu sa zemljom (6,74%), što se objašnjava variranjem u kvalitetu zemlje i prisustvu zemljišnih patogena. Obe ispitivane procedure su postigle visoku korelaciju sa nicanjem u polju. Rezultati ovih istraživanja ukazuju na mogućnost korišćenja peska kao supstrata u hladnom testu, čime bi se ispitivanje vigora semena kukuruza znatno olakšalo, a ujedno bi se stekli uslovi za standardizaciju hladnog testa.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines, Poređenje dve procedure hladnog testa za ispitivanje vigora semena samooplodnih linija kukuruza",
volume = "21",
number = "2",
pages = "115-117",
doi = "10.5937/JPEA1702115M",
url = "conv_647"
}
Milivojević, M., Srdić, J., Branković-Radojčić, D. V., Vukadinović, R., Kojić, J.,& Petrović, T.. (2017). Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 21(2), 115-117.
https://doi.org/10.5937/JPEA1702115M
conv_647
Milivojević M, Srdić J, Branković-Radojčić DV, Vukadinović R, Kojić J, Petrović T. Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines. in Journal on Processing and Energy in Agriculture. 2017;21(2):115-117.
doi:10.5937/JPEA1702115M
conv_647 .
Milivojević, Marija, Srdić, Jelena, Branković-Radojčić, Dragana V., Vukadinović, Radmila, Kojić, Jasna, Petrović, Tanja, "Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines" in Journal on Processing and Energy in Agriculture, 21, no. 2 (2017):115-117,
https://doi.org/10.5937/JPEA1702115M .,
conv_647 .
1

Variability of agronomic traits of maize hybrids influenced by the environmental factors

Branković-Radojčić, Dragana V.; Srdić, Jelena; Milivojević, Marija; Šurlan-Momirović, Gordana; Radojčić, Aleksandar; Živanović, Tomislav; Todorović, Goran

(Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad, 2017)

TY  - JOUR
AU  - Branković-Radojčić, Dragana V.
AU  - Srdić, Jelena
AU  - Milivojević, Marija
AU  - Šurlan-Momirović, Gordana
AU  - Radojčić, Aleksandar
AU  - Živanović, Tomislav
AU  - Todorović, Goran
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/661
AB  - In this study 36 maize hybrids of different FAO maturity groups were observed in three successive years (2011, 2012 and 2013), on 8 locations. The main objective of this experiment was to observe the GxE interaction concerning yield, grain moisture, grain yield per ear and test weight. The experiment was set up according to the RCBD. Based on the obtained results average estimates, CV and overall ranking of hybrids were calculated. ANOVA was applied in order to estimate the effect of factors: genotype, environment and interaction. Thus the significance of all these factors was observed. Results of this research indicate the importance and necessity of performing multilocation and multiyear trials with the aim of observation and understanding the intensity of GxE interaction, as well as its influence on the grain yield and it components.
AB  - Istraživanje je obuhvatilo 36 hibrida različitih FAO grupa zrenja, u 2011, 2012 i 2013 godini, na 8 lokaliteta. U ogledu je ispitivana interakcija genotip x sredina u pogledu stabilnosti prinosa zrna, vlage zrna, težine zrna po klipu i zapreminske mase kukuruza. Ogled je bio postavljen potpuno slučajnom blok sistemu (RCBD). Na osnovu dobijenih podataka izračunate su prosečne vrednosti, pokazatelji varijabilnosti posmatranih osobina i ukupan rang hibrida, a analizom varijanse utvrđena je značajnost efekta genotipa, sredina i interakcija. Za sve proučavane agronomske osobine, analizom varijanse su utvrđene statistički visokoznačajne vrednosti genotipa, sredina i interakcije. Najviši prosečan prinos zrna u ogledu ostvaren je u 2011. godini (11,62 t/ha), a najniži u 2012. godini (6,90 t/ha). Najniži prosečan procenat vlage zrna kukuruza u ogledu ostvaren je u veoma sušnoj 2012. godini (14,86%), dok između vrednosti ostvarenih u 2011. godini (19,47%) i 2013. godini (19,52%) nije bilo značajnije razlike. Vrednosti težine zrna po klipu kukuruza bile su direktno srazmerne vrednostima prinosa. Najveća zapreminska masa zrna kukuruza ostvarena je u sušnoj 2012. godini (74,84 kg/hl), dok je najmanje izmerena u kišovitoj 2013. godini (70,47 kg/hl). Rezultati ovog istraživanja potvrđuju neophodnost izvođenja višelokacijskih i višegodišnjih ogleda u cilju što boljeg sagledavanja intenziteta interakcija genotip x sredina, i njihovog uticaja na prinos i komponente prinosa hibrida kukuruza različitih FAO grupa zrenja na teritoriji Srbije. Da bi proizvodnja kukuruza bila stabilna, farmerima koji nemaju mogućnost navodnjavanja, već kukuruz gaje u suvom ratarenju, treba savetovati da seju hibride različite dužine vegetacije.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Variability of agronomic traits of maize hybrids influenced by the environmental factors
T1  - Varijabilnost agronomskih osobina hibrida kukuruza pod uticajem faktora sredine
VL  - 21
IS  - 3
SP  - 149
EP  - 153
DO  - 10.5937/JPEA1703149B
UR  - conv_649
ER  - 
@article{
author = "Branković-Radojčić, Dragana V. and Srdić, Jelena and Milivojević, Marija and Šurlan-Momirović, Gordana and Radojčić, Aleksandar and Živanović, Tomislav and Todorović, Goran",
year = "2017",
abstract = "In this study 36 maize hybrids of different FAO maturity groups were observed in three successive years (2011, 2012 and 2013), on 8 locations. The main objective of this experiment was to observe the GxE interaction concerning yield, grain moisture, grain yield per ear and test weight. The experiment was set up according to the RCBD. Based on the obtained results average estimates, CV and overall ranking of hybrids were calculated. ANOVA was applied in order to estimate the effect of factors: genotype, environment and interaction. Thus the significance of all these factors was observed. Results of this research indicate the importance and necessity of performing multilocation and multiyear trials with the aim of observation and understanding the intensity of GxE interaction, as well as its influence on the grain yield and it components., Istraživanje je obuhvatilo 36 hibrida različitih FAO grupa zrenja, u 2011, 2012 i 2013 godini, na 8 lokaliteta. U ogledu je ispitivana interakcija genotip x sredina u pogledu stabilnosti prinosa zrna, vlage zrna, težine zrna po klipu i zapreminske mase kukuruza. Ogled je bio postavljen potpuno slučajnom blok sistemu (RCBD). Na osnovu dobijenih podataka izračunate su prosečne vrednosti, pokazatelji varijabilnosti posmatranih osobina i ukupan rang hibrida, a analizom varijanse utvrđena je značajnost efekta genotipa, sredina i interakcija. Za sve proučavane agronomske osobine, analizom varijanse su utvrđene statistički visokoznačajne vrednosti genotipa, sredina i interakcije. Najviši prosečan prinos zrna u ogledu ostvaren je u 2011. godini (11,62 t/ha), a najniži u 2012. godini (6,90 t/ha). Najniži prosečan procenat vlage zrna kukuruza u ogledu ostvaren je u veoma sušnoj 2012. godini (14,86%), dok između vrednosti ostvarenih u 2011. godini (19,47%) i 2013. godini (19,52%) nije bilo značajnije razlike. Vrednosti težine zrna po klipu kukuruza bile su direktno srazmerne vrednostima prinosa. Najveća zapreminska masa zrna kukuruza ostvarena je u sušnoj 2012. godini (74,84 kg/hl), dok je najmanje izmerena u kišovitoj 2013. godini (70,47 kg/hl). Rezultati ovog istraživanja potvrđuju neophodnost izvođenja višelokacijskih i višegodišnjih ogleda u cilju što boljeg sagledavanja intenziteta interakcija genotip x sredina, i njihovog uticaja na prinos i komponente prinosa hibrida kukuruza različitih FAO grupa zrenja na teritoriji Srbije. Da bi proizvodnja kukuruza bila stabilna, farmerima koji nemaju mogućnost navodnjavanja, već kukuruz gaje u suvom ratarenju, treba savetovati da seju hibride različite dužine vegetacije.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Variability of agronomic traits of maize hybrids influenced by the environmental factors, Varijabilnost agronomskih osobina hibrida kukuruza pod uticajem faktora sredine",
volume = "21",
number = "3",
pages = "149-153",
doi = "10.5937/JPEA1703149B",
url = "conv_649"
}
Branković-Radojčić, D. V., Srdić, J., Milivojević, M., Šurlan-Momirović, G., Radojčić, A., Živanović, T.,& Todorović, G.. (2017). Variability of agronomic traits of maize hybrids influenced by the environmental factors. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 21(3), 149-153.
https://doi.org/10.5937/JPEA1703149B
conv_649
Branković-Radojčić DV, Srdić J, Milivojević M, Šurlan-Momirović G, Radojčić A, Živanović T, Todorović G. Variability of agronomic traits of maize hybrids influenced by the environmental factors. in Journal on Processing and Energy in Agriculture. 2017;21(3):149-153.
doi:10.5937/JPEA1703149B
conv_649 .
Branković-Radojčić, Dragana V., Srdić, Jelena, Milivojević, Marija, Šurlan-Momirović, Gordana, Radojčić, Aleksandar, Živanović, Tomislav, Todorović, Goran, "Variability of agronomic traits of maize hybrids influenced by the environmental factors" in Journal on Processing and Energy in Agriculture, 21, no. 3 (2017):149-153,
https://doi.org/10.5937/JPEA1703149B .,
conv_649 .
1

Germination test methods of insecticide-treated seeds

Kojić, Jasna; Milivojević, Marija; Petrović, Tanja; Branković-Radojčić, Dragana V.; Jovanović, Snežana; Tabaković, Marijenka

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, 2016)

TY  - JOUR
AU  - Kojić, Jasna
AU  - Milivojević, Marija
AU  - Petrović, Tanja
AU  - Branković-Radojčić, Dragana V.
AU  - Jovanović, Snežana
AU  - Tabaković, Marijenka
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/906
AB  - The seed treatment with insecticides in certain cases causes lower values of seed germination due
to phytotoxicity, which is manifested as a truncated and thickened main root. In the Seed Testing
Laboratory, the method most suitable for seed germination was sought out in order to reduce
effects of phytotoxicity of treated seeds to the minimum. This study encompassed germination
tests of 10 seed lots of maize hybrids of different maturity groups stored in the controlledenvironment chamber for a year. One part of seeds was treated with the fungicide Maxim XL,
while the remaining part was treated with the fungicide Maxim XL + insecticide Sonido. The
seed germination tests were carried out in the Seed Testing Laboratory, Maize Research Institute,
Zemun Polje, by the between paper method (4x100 and 8x50 seeds) and the sand method (4x100
seeds). Seeds were germinated in the room germinator at the alternating temperature of
20<=>30oC and 16:8 (light : dark) photoperiod. The first counting was done on the fourth day
and the final one on the seventh day when the BP method was used. In cases when the S method
was applied, the final counting was done on the seventh day. When seeds were germinated, the
control sample was also placed for germination. Considering results of the last year germination,
it was determined that seed germination was not significantly changed after a year of storing in
the controlled-environment chamber. The comparison of results on germination of seeds treated
only with the fungicide with seeds treated with fungicide+insecticide, shows lower values in seed
germination in all three treatments in fungicide+insecticide-treated seeds. The lowest germination
values were recorded in seeds treated with fungicide+insecticide in sand, while the highest values
were obtained by the BP method (8x50 seeds).
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture
T2  - 7. International scientific agricultural symposium „Agrosym 2016“ , October 06-09, 2016. Jahorina - Book of proceedings
T1  - Germination test methods of insecticide-treated seeds
SP  - 946
EP  - 949
DO  - 10.7251/AGRENG1607140
ER  - 
@article{
author = "Kojić, Jasna and Milivojević, Marija and Petrović, Tanja and Branković-Radojčić, Dragana V. and Jovanović, Snežana and Tabaković, Marijenka",
year = "2016",
abstract = "The seed treatment with insecticides in certain cases causes lower values of seed germination due
to phytotoxicity, which is manifested as a truncated and thickened main root. In the Seed Testing
Laboratory, the method most suitable for seed germination was sought out in order to reduce
effects of phytotoxicity of treated seeds to the minimum. This study encompassed germination
tests of 10 seed lots of maize hybrids of different maturity groups stored in the controlledenvironment chamber for a year. One part of seeds was treated with the fungicide Maxim XL,
while the remaining part was treated with the fungicide Maxim XL + insecticide Sonido. The
seed germination tests were carried out in the Seed Testing Laboratory, Maize Research Institute,
Zemun Polje, by the between paper method (4x100 and 8x50 seeds) and the sand method (4x100
seeds). Seeds were germinated in the room germinator at the alternating temperature of
20<=>30oC and 16:8 (light : dark) photoperiod. The first counting was done on the fourth day
and the final one on the seventh day when the BP method was used. In cases when the S method
was applied, the final counting was done on the seventh day. When seeds were germinated, the
control sample was also placed for germination. Considering results of the last year germination,
it was determined that seed germination was not significantly changed after a year of storing in
the controlled-environment chamber. The comparison of results on germination of seeds treated
only with the fungicide with seeds treated with fungicide+insecticide, shows lower values in seed
germination in all three treatments in fungicide+insecticide-treated seeds. The lowest germination
values were recorded in seeds treated with fungicide+insecticide in sand, while the highest values
were obtained by the BP method (8x50 seeds).",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture",
journal = "7. International scientific agricultural symposium „Agrosym 2016“ , October 06-09, 2016. Jahorina - Book of proceedings",
title = "Germination test methods of insecticide-treated seeds",
pages = "946-949",
doi = "10.7251/AGRENG1607140"
}
Kojić, J., Milivojević, M., Petrović, T., Branković-Radojčić, D. V., Jovanović, S.,& Tabaković, M.. (2016). Germination test methods of insecticide-treated seeds. in 7. International scientific agricultural symposium „Agrosym 2016“ , October 06-09, 2016. Jahorina - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture., 946-949.
https://doi.org/10.7251/AGRENG1607140
Kojić J, Milivojević M, Petrović T, Branković-Radojčić DV, Jovanović S, Tabaković M. Germination test methods of insecticide-treated seeds. in 7. International scientific agricultural symposium „Agrosym 2016“ , October 06-09, 2016. Jahorina - Book of proceedings. 2016;:946-949.
doi:10.7251/AGRENG1607140 .
Kojić, Jasna, Milivojević, Marija, Petrović, Tanja, Branković-Radojčić, Dragana V., Jovanović, Snežana, Tabaković, Marijenka, "Germination test methods of insecticide-treated seeds" in 7. International scientific agricultural symposium „Agrosym 2016“ , October 06-09, 2016. Jahorina - Book of proceedings (2016):946-949,
https://doi.org/10.7251/AGRENG1607140 . .

Ring analyses in the proficiency assessment of authorised seed samplers in the Republic of Serbia

Kojić, Jasna; Stevanović, Nikola; Petrović, Tanja; Milivojević, Marija; Jovanović, Snežana; Tabaković, Marijenka

(Novi Sad : National Society of Processing and Energy in Agriculture, 2016)

TY  - JOUR
AU  - Kojić, Jasna
AU  - Stevanović, Nikola
AU  - Petrović, Tanja
AU  - Milivojević, Marija
AU  - Jovanović, Snežana
AU  - Tabaković, Marijenka
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/878
AB  - The objective of this study was to apply the ring analysis in the proficiency assessment of authorised seed samplers form 18
accredited laboratories from the Republic of Serbia and two seed samplers from the Republic of Macedonia. The seed sampling was
performed in the Processing plant, while the assessment was performed at the Seed Testing Laboratory of the Maize Research
Institute, Zemun Polje. Two maize seed lots, placed in containers and jumbo bags, and one barley seed lot packed in paper bags,
were sampled. Drawing of submitted samples and the estimation of the sampling intensity by seed samplers were done during the
course of sampling. Seed purity, 1000-seed weight and seed germination were determined in the drawn samples. Results obtained by
authorised samplers were within toleration limits.
All samplers from accredited laboratories proved to be well trained, the samples were properly drawn, while insignificant
nonconformities were detected in the calculation of sampling intensity.
AB  - Cilj ovog rada je primena kružne analize u kontroli ovlašćenih uzorkovača semena iz 18 akreditovanih laboratorija iz Republik e
Srbije i dva uzorkovača iz Makedonije. Uzorkovanje i ispitivanje obavljeno je na Doradnom centru i u Laboratoriji za ispiti vanje
semena Instituta za kukuruz „Zemun Polje“. Uzorkovane su dve partije semena kukuruza, smeštene u kontejnerima i džambo
vrećama i jedna partija ječma upakovana u papirne vreće. U toku uzorkovanja, praćen je način formiranj a prosečnog uzorka i
izračunavanja intenziteta uzorkovanja od strane uzorkovača. Na uzetim uzorcima ispitivana je čistoća semena, masa 1000 semena i
klijavost semena. Utvrđivanje klijavosti semena kukuruza je izvršeno na standardnom temperaturnom režimu 20<=>30 0C,
naizmenično 8/16 h, a za seme ječma na 200C. Masa je utvrđena merenjem 8x100 semena a čistoća semena procentualnim učešćem
nečistoća u uzorku. Ispitivana čistoća semena se kretala od 99,4%-99,9% za prvi uzorak kukuruza, za drugi uzorak kukuruza se
kretala od 98,6-99,3% a za uzorak ječma od 99,4-99,7%. Klijavost semena za prvi uzorak kukuruza se kretala od 96-98%, za drugi
uzorak od 90-96% a za partiju ječma od 97-99%. Masa se kretala od 351,7 g-357,3 g za prvi uzorak kukuruza, za drugi uzorak
kukuruza od 306,3 g-315,0 g a za uzorak ječma od 46,19 g-44,76 g. Analize su rađene prema Pravilnika o kvalitetu semena
poljoprivrednog bilja 47/87 i po međunarodnim ISTA pravilima. Eksperimentalni podaci obrađeni su deskriptivnom statistikom
(srednja vrednost, standardan devijacija, medijana), koristeći Data Analisysis Softver System.
Dobijeni rezultati ovlašćenih uzorkovača su u granicama dozvoljenih odstupanja. Svi uzorkovači iz akreditovanih laboratorija
dobro formiraju radni uzorak, dok su manja odstupanja uočena kod izračunavanja intenziteta uzorkovanja.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Ring analyses in the proficiency assessment of authorised seed samplers in the Republic of Serbia
T1  - Kružne analize u proveri rada autorizovanih uzorkovača semena
VL  - 20
IS  - 1
SP  - 9
EP  - 12
ER  - 
@article{
author = "Kojić, Jasna and Stevanović, Nikola and Petrović, Tanja and Milivojević, Marija and Jovanović, Snežana and Tabaković, Marijenka",
year = "2016",
abstract = "The objective of this study was to apply the ring analysis in the proficiency assessment of authorised seed samplers form 18
accredited laboratories from the Republic of Serbia and two seed samplers from the Republic of Macedonia. The seed sampling was
performed in the Processing plant, while the assessment was performed at the Seed Testing Laboratory of the Maize Research
Institute, Zemun Polje. Two maize seed lots, placed in containers and jumbo bags, and one barley seed lot packed in paper bags,
were sampled. Drawing of submitted samples and the estimation of the sampling intensity by seed samplers were done during the
course of sampling. Seed purity, 1000-seed weight and seed germination were determined in the drawn samples. Results obtained by
authorised samplers were within toleration limits.
All samplers from accredited laboratories proved to be well trained, the samples were properly drawn, while insignificant
nonconformities were detected in the calculation of sampling intensity., Cilj ovog rada je primena kružne analize u kontroli ovlašćenih uzorkovača semena iz 18 akreditovanih laboratorija iz Republik e
Srbije i dva uzorkovača iz Makedonije. Uzorkovanje i ispitivanje obavljeno je na Doradnom centru i u Laboratoriji za ispiti vanje
semena Instituta za kukuruz „Zemun Polje“. Uzorkovane su dve partije semena kukuruza, smeštene u kontejnerima i džambo
vrećama i jedna partija ječma upakovana u papirne vreće. U toku uzorkovanja, praćen je način formiranj a prosečnog uzorka i
izračunavanja intenziteta uzorkovanja od strane uzorkovača. Na uzetim uzorcima ispitivana je čistoća semena, masa 1000 semena i
klijavost semena. Utvrđivanje klijavosti semena kukuruza je izvršeno na standardnom temperaturnom režimu 20<=>30 0C,
naizmenično 8/16 h, a za seme ječma na 200C. Masa je utvrđena merenjem 8x100 semena a čistoća semena procentualnim učešćem
nečistoća u uzorku. Ispitivana čistoća semena se kretala od 99,4%-99,9% za prvi uzorak kukuruza, za drugi uzorak kukuruza se
kretala od 98,6-99,3% a za uzorak ječma od 99,4-99,7%. Klijavost semena za prvi uzorak kukuruza se kretala od 96-98%, za drugi
uzorak od 90-96% a za partiju ječma od 97-99%. Masa se kretala od 351,7 g-357,3 g za prvi uzorak kukuruza, za drugi uzorak
kukuruza od 306,3 g-315,0 g a za uzorak ječma od 46,19 g-44,76 g. Analize su rađene prema Pravilnika o kvalitetu semena
poljoprivrednog bilja 47/87 i po međunarodnim ISTA pravilima. Eksperimentalni podaci obrađeni su deskriptivnom statistikom
(srednja vrednost, standardan devijacija, medijana), koristeći Data Analisysis Softver System.
Dobijeni rezultati ovlašćenih uzorkovača su u granicama dozvoljenih odstupanja. Svi uzorkovači iz akreditovanih laboratorija
dobro formiraju radni uzorak, dok su manja odstupanja uočena kod izračunavanja intenziteta uzorkovanja.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Ring analyses in the proficiency assessment of authorised seed samplers in the Republic of Serbia, Kružne analize u proveri rada autorizovanih uzorkovača semena",
volume = "20",
number = "1",
pages = "9-12"
}
Kojić, J., Stevanović, N., Petrović, T., Milivojević, M., Jovanović, S.,& Tabaković, M.. (2016). Ring analyses in the proficiency assessment of authorised seed samplers in the Republic of Serbia. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 20(1), 9-12.
Kojić J, Stevanović N, Petrović T, Milivojević M, Jovanović S, Tabaković M. Ring analyses in the proficiency assessment of authorised seed samplers in the Republic of Serbia. in Journal on Processing and Energy in Agriculture. 2016;20(1):9-12..
Kojić, Jasna, Stevanović, Nikola, Petrović, Tanja, Milivojević, Marija, Jovanović, Snežana, Tabaković, Marijenka, "Ring analyses in the proficiency assessment of authorised seed samplers in the Republic of Serbia" in Journal on Processing and Energy in Agriculture, 20, no. 1 (2016):9-12.

Kvalitet semena samooplodnih linija kukuruza različitih grupa zrenja i genetičke osnove

Milivojević, Marija

(Универзитет у Новом Саду, Пољопривредни факултет, 2016)

TY  - THES
AU  - Milivojević, Marija
PY  - 2016
UR  - http://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija146770944774460.pdf?controlNumber=(BISIS)101400&fileName=146770944774460.pdf&id=6254&source=NaRDuS&language=sr
UR  - http://www.cris.uns.ac.rs/record.jsf?recordId=101400&source=NaRDuS&language=sr
UR  - http://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije146770945842386.pdf?controlNumber=(BISIS)101400&fileName=146770945842386.pdf&id=6255&source=NaRDuS&language=sr
UR  - /DownloadFileServlet/IzvestajKomisije146770945842386.pdf?controlNumber=(BISIS)101400&fileName=146770945842386.pdf&id=6255
UR  - http://nardus.mpn.gov.rs/123456789/8754
UR  - http://rik.mrizp.rs/handle/123456789/755
AB  - U radu su sprovedena ispitivanja kvaliteta semena samooplodnih linija kukuruza stvorenih u Institutu za kukuruz „Zemun Polje“ razliĉitih grupa zrenja i genetiĉke osnove. U laboratorijskim i poljskim uslovima utvrĊena je klijavost i vigor semena iz 2011. nakon ĉetiri godine ĉuvanja u kontrolisanim uslovima (18 °C i 60% RH) i semena iz 2014. nakon godinu dana ĉuvanja na 5 °C i 60% RH. Cilj je bio utvrditi tolerantnost odabranih linija na niske temperature prilikom klijanja i nicanja, dugoveĉnost semena u kontrolisanim uslovima ĉuvanja, genetiĉku divergentnost linija primenom proteinskih markera i proceniti uticaj genetiĉke osnove na kvalitet semena. U laboratoriji za ispitivanje semena praćeni su sledeći parametri kvaliteta: vitalnost, klijavost, energija klijanja, dužina klijanaca, vigor ispitan tetrazolium testom, hladnim testom, testom ubrzanog starenja, testom pojave primarnog korena i testom provodljivosti. Primenom vigor testova na semenu dva nivoa starosti omogućena je bolja klasifikacija linija u pogledu kvaliteta, nego standardnim testom klijavosti. Rezultati rada ukazuju da su hladni test i test ubrzanog starenja najpogodniji za procenu vigora semena u laboratoriji, s tim da je hladni test bolji pokazatelj nicanja u polju. Ispitivane samooplodne linije pokazale su dobru sposobnost oĉuvanja kvaliteta semena kao i visok nivo toleratnosti na niske temperature u toku klijanja i ranog porasta biljaka. Kontrolisani uslovi ĉuvanja na 18 °C omogućili su da se klijavost i vigor semena u ispitivanom periodu, održi na visokom nivou. Dužina vegetacije samooplodnih linija nije imala znaĉajan uticaj na klijavost i vigor semena. Kvalitet semena ispitivanih samooplodnih linija je prvenstveno bio pod uticajem genetiĉke osnove. Linije poreklom od Lancaster populacije imale su slabiji vigor u odnosu na linije BSSS i Iowa Dent porekla. U radu je potvrĊeno da vigor testovi imaju važnu ulogu u ispitivanju kvaliteta semena samooplodnih linija kukuruza. Dobijeni rezultati direktno se mogu iskoristiti u oplemenjivaĉkim programima u cilju poboljšanja klijavosti i vigora semena. Ostvareni rezultati ukazuju da su informacije o genetiĉkom poreklu samooplodnih linija važan indikator vigora semena. Pored podataka o genetiĉkom poreklu linija veliki znaĉaj ima karakterizacija putem biohemijskih markera.
AB  - The extensive seed quality evaluations of maize inbred lines developed at the Maize Research Institute Zemun Polje of different maturity groups and genetic background were conducted. In laboratory and field conditions germination and vigour of seed produced in 2011 and 2014 was determined, after four years of storage at 18 °C, 60% RH and one year of storage at 5 °C, 60% RH, respectively. The objective of this research was to determine the chilling tolerance of selected inbred lines during germination and emergence, seed longevity under controlled storage conditions, genetic divergence determined by biochemical markers and to assess the impact of the genetic background on seed quality. Following seed quality parameters were tested: viability, germination, seedling length and vigour examined by cold test, accelerated ageing test, tetrazolium test, radicle emergence test and conductivity test. Vigour tests provided a better classification of inbred lines of different seed age in terms of quality than standard germination test. Obtained results show that cold test and accelerated aging test are the most suitable for assessing seed vigour in the laboratory, but cold test is a better indicator of field emergence. Tested maize inbred lines showed good seed storability and high level of chilling tolerance during germination and early growth of plants. Controlled storage conditions at 18 °C, have enabled that germination and seed vigor remains at the high level in the examined period. Seed germination and vigour of maize inbred lines were not affected by length of vegetation. Seed quality was primarily influenced by genetic background of tested inbred lines. Inbred lines originating from Lancaster populations had lower vigour compared to lines with BSSS and Iowa Dent origin. This research confirmed that vigor tests play an important role in seed quality testing of maize inbred lines. Results of this work can be directly used in breeding programs to improve germination and seed vigour. Achieved results indicate that information about the genetic origin of inbred lines is an important indicator of seed vigour. Finally, data emphasized the importance of inbred line characterization by biochemical markers in addition to genetic origin information.
PB  - Универзитет у Новом Саду, Пољопривредни факултет
T2  - Универзитет у Новом Саду
T1  - Kvalitet semena samooplodnih linija kukuruza različitih grupa zrenja i genetičke osnove
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8754
ER  - 
@phdthesis{
author = "Milivojević, Marija",
year = "2016",
abstract = "U radu su sprovedena ispitivanja kvaliteta semena samooplodnih linija kukuruza stvorenih u Institutu za kukuruz „Zemun Polje“ razliĉitih grupa zrenja i genetiĉke osnove. U laboratorijskim i poljskim uslovima utvrĊena je klijavost i vigor semena iz 2011. nakon ĉetiri godine ĉuvanja u kontrolisanim uslovima (18 °C i 60% RH) i semena iz 2014. nakon godinu dana ĉuvanja na 5 °C i 60% RH. Cilj je bio utvrditi tolerantnost odabranih linija na niske temperature prilikom klijanja i nicanja, dugoveĉnost semena u kontrolisanim uslovima ĉuvanja, genetiĉku divergentnost linija primenom proteinskih markera i proceniti uticaj genetiĉke osnove na kvalitet semena. U laboratoriji za ispitivanje semena praćeni su sledeći parametri kvaliteta: vitalnost, klijavost, energija klijanja, dužina klijanaca, vigor ispitan tetrazolium testom, hladnim testom, testom ubrzanog starenja, testom pojave primarnog korena i testom provodljivosti. Primenom vigor testova na semenu dva nivoa starosti omogućena je bolja klasifikacija linija u pogledu kvaliteta, nego standardnim testom klijavosti. Rezultati rada ukazuju da su hladni test i test ubrzanog starenja najpogodniji za procenu vigora semena u laboratoriji, s tim da je hladni test bolji pokazatelj nicanja u polju. Ispitivane samooplodne linije pokazale su dobru sposobnost oĉuvanja kvaliteta semena kao i visok nivo toleratnosti na niske temperature u toku klijanja i ranog porasta biljaka. Kontrolisani uslovi ĉuvanja na 18 °C omogućili su da se klijavost i vigor semena u ispitivanom periodu, održi na visokom nivou. Dužina vegetacije samooplodnih linija nije imala znaĉajan uticaj na klijavost i vigor semena. Kvalitet semena ispitivanih samooplodnih linija je prvenstveno bio pod uticajem genetiĉke osnove. Linije poreklom od Lancaster populacije imale su slabiji vigor u odnosu na linije BSSS i Iowa Dent porekla. U radu je potvrĊeno da vigor testovi imaju važnu ulogu u ispitivanju kvaliteta semena samooplodnih linija kukuruza. Dobijeni rezultati direktno se mogu iskoristiti u oplemenjivaĉkim programima u cilju poboljšanja klijavosti i vigora semena. Ostvareni rezultati ukazuju da su informacije o genetiĉkom poreklu samooplodnih linija važan indikator vigora semena. Pored podataka o genetiĉkom poreklu linija veliki znaĉaj ima karakterizacija putem biohemijskih markera., The extensive seed quality evaluations of maize inbred lines developed at the Maize Research Institute Zemun Polje of different maturity groups and genetic background were conducted. In laboratory and field conditions germination and vigour of seed produced in 2011 and 2014 was determined, after four years of storage at 18 °C, 60% RH and one year of storage at 5 °C, 60% RH, respectively. The objective of this research was to determine the chilling tolerance of selected inbred lines during germination and emergence, seed longevity under controlled storage conditions, genetic divergence determined by biochemical markers and to assess the impact of the genetic background on seed quality. Following seed quality parameters were tested: viability, germination, seedling length and vigour examined by cold test, accelerated ageing test, tetrazolium test, radicle emergence test and conductivity test. Vigour tests provided a better classification of inbred lines of different seed age in terms of quality than standard germination test. Obtained results show that cold test and accelerated aging test are the most suitable for assessing seed vigour in the laboratory, but cold test is a better indicator of field emergence. Tested maize inbred lines showed good seed storability and high level of chilling tolerance during germination and early growth of plants. Controlled storage conditions at 18 °C, have enabled that germination and seed vigor remains at the high level in the examined period. Seed germination and vigour of maize inbred lines were not affected by length of vegetation. Seed quality was primarily influenced by genetic background of tested inbred lines. Inbred lines originating from Lancaster populations had lower vigour compared to lines with BSSS and Iowa Dent origin. This research confirmed that vigor tests play an important role in seed quality testing of maize inbred lines. Results of this work can be directly used in breeding programs to improve germination and seed vigour. Achieved results indicate that information about the genetic origin of inbred lines is an important indicator of seed vigour. Finally, data emphasized the importance of inbred line characterization by biochemical markers in addition to genetic origin information.",
publisher = "Универзитет у Новом Саду, Пољопривредни факултет",
journal = "Универзитет у Новом Саду",
title = "Kvalitet semena samooplodnih linija kukuruza različitih grupa zrenja i genetičke osnove",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8754"
}
Milivojević, M.. (2016). Kvalitet semena samooplodnih linija kukuruza različitih grupa zrenja i genetičke osnove. in Универзитет у Новом Саду
Универзитет у Новом Саду, Пољопривредни факултет..
https://hdl.handle.net/21.15107/rcub_nardus_8754
Milivojević M. Kvalitet semena samooplodnih linija kukuruza različitih grupa zrenja i genetičke osnove. in Универзитет у Новом Саду. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_8754 .
Milivojević, Marija, "Kvalitet semena samooplodnih linija kukuruza različitih grupa zrenja i genetičke osnove" in Универзитет у Новом Саду (2016),
https://hdl.handle.net/21.15107/rcub_nardus_8754 .

Detection of Rhizomucor pusillus on sunflower seed

Lević, Jelena; Ivanović, D.; Stanković, Slavica; Milivojević, Marija; Vukadinović, Radmila; Stepanić, Ana

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Ivanović, D.
AU  - Stanković, Slavica
AU  - Milivojević, Marija
AU  - Vukadinović, Radmila
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/478
AB  - The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - Detection of Rhizomucor pusillus on sunflower seed
VL  - 36
IS  - 59
SP  - 59
EP  - 70
DO  - 10.2298/HEL1359059L
UR  - conv_513
ER  - 
@article{
author = "Lević, Jelena and Ivanović, D. and Stanković, Slavica and Milivojević, Marija and Vukadinović, Radmila and Stepanić, Ana",
year = "2013",
abstract = "The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Detection of Rhizomucor pusillus on sunflower seed",
volume = "36",
number = "59",
pages = "59-70",
doi = "10.2298/HEL1359059L",
url = "conv_513"
}
Lević, J., Ivanović, D., Stanković, S., Milivojević, M., Vukadinović, R.,& Stepanić, A.. (2013). Detection of Rhizomucor pusillus on sunflower seed. in Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 36(59), 59-70.
https://doi.org/10.2298/HEL1359059L
conv_513
Lević J, Ivanović D, Stanković S, Milivojević M, Vukadinović R, Stepanić A. Detection of Rhizomucor pusillus on sunflower seed. in Helia. 2013;36(59):59-70.
doi:10.2298/HEL1359059L
conv_513 .
Lević, Jelena, Ivanović, D., Stanković, Slavica, Milivojević, Marija, Vukadinović, Radmila, Stepanić, Ana, "Detection of Rhizomucor pusillus on sunflower seed" in Helia, 36, no. 59 (2013):59-70,
https://doi.org/10.2298/HEL1359059L .,
conv_513 .

Thermodynamic characterisation of wheat seedlings, originating from seeds from different fields

Dragičević, Vesna; Ivanović, Dragica; Milivojević, Marija; Đukanović, Lana

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2012)

TY  - JOUR
AU  - Dragičević, Vesna
AU  - Ivanović, Dragica
AU  - Milivojević, Marija
AU  - Đukanović, Lana
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/448
AB  - Wheat germination could be affected by different factors, including location. Start growth of seedlings present the energy transformations and the seed substance conversion. The aim of the experiment was to examine differences in germination and growth of wheat seedlings, originating from five fields with similar characteristics, with application of thermodynamic parameters of free energy and entropy. Variations in energy and total germination of wheat seed was under 10%. After eight days of germination testing, the relation between root and shoot at level of fresh and dry matter was 1:1, while only at seedlings from one field it was 1:2 in fresh and 1.5:1 in dry matter. The present differences could be the consequence of the different energy using in seedling. Hydrolysis intensification based on spontaneous reactions and increased order in four days old seedlings could result in increased germination. On the other hand, variations in energetic potential, order degree and hydrolysis of seed substance may not take off biosynthetic reactions in root and shoot. Present data towards that variation in growth of seedlings originating from seeds from different fields could be mainly connected to variations in energy potential inputted by water, but not by synthesized substance. Minimal differences between the individual fields could affect quality of the produced seed material, what was particularly underlined by application of thermodynamic parameters as quality indicators.
AB  - Brojni faktori utiču na klijavost semena pšenice, među koje spada i lokacija. Početni rast klijanaca predstavlja rezultat energetskih transformacija i konverzije semene supstance. Cilj ogleda je bio da se ispitaju razlike u klijavosti i razvoju klijanaca pšenice poreklom iz semena dobijenog sa pet različitih parcela (koje imaju slične osobine), uz primenu termodinamičkih parametara slobodne energije i entropije. Variranje u energiji klijanja i ukupnoj klijavosti semena pšenice nije bilo veće od 10%. Nakon osam dana od početka testiranja klijavosti kod većine klijanaca je odnos između korena i izdanka na nivou sveže i suve materije bio 1:1, dok je jedino kod klijanaca poreklom iz semena samo sa jedne parcele ovaj odnos na nivou sveže materije bio skoro 1:2, a na nivou suve materije 1,5:1. Navedene razlike mogu biti posledica različitog iskorišćenja energije od strane samog klijanca. Intenziviranje hidrolize, na bazi spontanih reakcija, kao i povećane uređenosti kod klijanaca starih četiri dana bi moglo da rezultira nešto većom ukupnom klijavošću. Sa druge strane, variranja u energetskom potencijalu, stepenu uređenosti sistema i hidrolizi semene supstance ne moraju da se odraze i na biosintetske reakcije u korenu i izdanku. Navedeni podaci upućuju da su variranja u rastu klijanaca poreklom iz semena sa različitih parcela uglavnom vezana za variranja energetskog potencijala koji je unet sa vodom, a ne i sintetisane supstance klijanaca. Minimalne razlike između pojedinačnih parcela mogu uticati na kvalitet proizvedenog semenskog materijala, što je posebno naglašeno primenom termodinamičkih parametra, kao pokazatelja kvaliteta.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Thermodynamic characterisation of wheat seedlings, originating from seeds from different fields
T1  - Termodinamička karakterizacija klijanaca pšenice poreklom iz semena sa različitih parcela
VL  - 18
IS  - 1
SP  - 17
EP  - 25
UR  - conv_285
ER  - 
@article{
author = "Dragičević, Vesna and Ivanović, Dragica and Milivojević, Marija and Đukanović, Lana",
year = "2012",
abstract = "Wheat germination could be affected by different factors, including location. Start growth of seedlings present the energy transformations and the seed substance conversion. The aim of the experiment was to examine differences in germination and growth of wheat seedlings, originating from five fields with similar characteristics, with application of thermodynamic parameters of free energy and entropy. Variations in energy and total germination of wheat seed was under 10%. After eight days of germination testing, the relation between root and shoot at level of fresh and dry matter was 1:1, while only at seedlings from one field it was 1:2 in fresh and 1.5:1 in dry matter. The present differences could be the consequence of the different energy using in seedling. Hydrolysis intensification based on spontaneous reactions and increased order in four days old seedlings could result in increased germination. On the other hand, variations in energetic potential, order degree and hydrolysis of seed substance may not take off biosynthetic reactions in root and shoot. Present data towards that variation in growth of seedlings originating from seeds from different fields could be mainly connected to variations in energy potential inputted by water, but not by synthesized substance. Minimal differences between the individual fields could affect quality of the produced seed material, what was particularly underlined by application of thermodynamic parameters as quality indicators., Brojni faktori utiču na klijavost semena pšenice, među koje spada i lokacija. Početni rast klijanaca predstavlja rezultat energetskih transformacija i konverzije semene supstance. Cilj ogleda je bio da se ispitaju razlike u klijavosti i razvoju klijanaca pšenice poreklom iz semena dobijenog sa pet različitih parcela (koje imaju slične osobine), uz primenu termodinamičkih parametara slobodne energije i entropije. Variranje u energiji klijanja i ukupnoj klijavosti semena pšenice nije bilo veće od 10%. Nakon osam dana od početka testiranja klijavosti kod većine klijanaca je odnos između korena i izdanka na nivou sveže i suve materije bio 1:1, dok je jedino kod klijanaca poreklom iz semena samo sa jedne parcele ovaj odnos na nivou sveže materije bio skoro 1:2, a na nivou suve materije 1,5:1. Navedene razlike mogu biti posledica različitog iskorišćenja energije od strane samog klijanca. Intenziviranje hidrolize, na bazi spontanih reakcija, kao i povećane uređenosti kod klijanaca starih četiri dana bi moglo da rezultira nešto većom ukupnom klijavošću. Sa druge strane, variranja u energetskom potencijalu, stepenu uređenosti sistema i hidrolizi semene supstance ne moraju da se odraze i na biosintetske reakcije u korenu i izdanku. Navedeni podaci upućuju da su variranja u rastu klijanaca poreklom iz semena sa različitih parcela uglavnom vezana za variranja energetskog potencijala koji je unet sa vodom, a ne i sintetisane supstance klijanaca. Minimalne razlike između pojedinačnih parcela mogu uticati na kvalitet proizvedenog semenskog materijala, što je posebno naglašeno primenom termodinamičkih parametra, kao pokazatelja kvaliteta.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Thermodynamic characterisation of wheat seedlings, originating from seeds from different fields, Termodinamička karakterizacija klijanaca pšenice poreklom iz semena sa različitih parcela",
volume = "18",
number = "1",
pages = "17-25",
url = "conv_285"
}
Dragičević, V., Ivanović, D., Milivojević, M.,& Đukanović, L.. (2012). Thermodynamic characterisation of wheat seedlings, originating from seeds from different fields. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 18(1), 17-25.
conv_285
Dragičević V, Ivanović D, Milivojević M, Đukanović L. Thermodynamic characterisation of wheat seedlings, originating from seeds from different fields. in Selekcija i semenarstvo. 2012;18(1):17-25.
conv_285 .
Dragičević, Vesna, Ivanović, Dragica, Milivojević, Marija, Đukanović, Lana, "Thermodynamic characterisation of wheat seedlings, originating from seeds from different fields" in Selekcija i semenarstvo, 18, no. 1 (2012):17-25,
conv_285 .

Effect of multiple alleles on oxidative stability and germination of soybean seeds subsequent to the accelerated ageing test

Žilić, Slađana; Milivojević, Marija; Šobajić, Slađana; Maksimović, Milan

(Društvo genetičara Srbije, Beograd, 2006)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Milivojević, Marija
AU  - Šobajić, Slađana
AU  - Maksimović, Milan
PY  - 2006
UR  - http://rik.mrizp.rs/handle/123456789/131
AB  - The purpose of this study was to determine changes of lipoxygenase activities, contents of antioxidants (tocopherol α, β-carotene and chlorophyll) fatty acids and soluble proteins, as well as, vigor in accelerated aged soybean seeds. The following soybean cultivars were used in the study: Williams 82, Goyou Kurakake and L93-7290. Subsequent to the accelerated ageing test (AAT), 23% of seeds of the cultivar Goyou Kurakake retained normal germination, while grain of the two remaining cultivars L93-7290 and Williams 82, completely lost germination ability. According to our results, the seeds of the cultivar Goyou Kurakake (the absence of lipoxygenases 2) is characterized by a significantly higher content of all observed antioxidants (tocopherol α 2.7 mg 100g-1, β-carotene 6.1 µg g-1 chlorophyll 4.9 µg g-1). The contents of (β-carotene and chlorophyll decreased after AAT in the seed of the cultivar Goyou Kurakake by 42.8% and 60.5%, respectively, while the content of tocopherol a remained the same. Furthermore, the tocopherol a content was not changed after AAT neither in the cultivar L93-7290 nor in the cultivar Williams 82. The content of β-carotene after AAT remained the same in seeds of the cultivar Williams 82 while it decreased by 7.7% in the cultivar L93-7290. Since changes of the fatty acids content were not pronounced in the cultivar Goyou Kurakake after AAT it can be concluded that the antioxidative system had a crucial role in seeds protection against lipid peroxidation.
AB  - Svrha ovih istraživanja bila je da se odrede promene aktivnosti izoenzima lipoksigenaze, sadržaja antioksidanasa (tokoferola α, ß-karotina i hlorofila), masnih kiselina i rastvorljivih proteina, kao i životne sposobnosti semena soje nakon ubrzanog starenja. Za istraživanja su korišćene sledeće sorte soje: Williams 82, Goyou Kurakake and L93-7290. Nakon testa ubrzanog starenja 23% semena sorte Goyou Kurakake zadržalo je normalnu klijavost, dok je seme sorti Williams 82 i L93-7290 potpuno izgubilo klijavost. Na osnovu naših rezultata sorta Goyou Kurakake, koja se odlikuje odsustvom lipoksigenaze 2, imala je značajno viši sadržaj ispitivanih antioksidanasa (tokofherol α 2,7 mg 100g-1, ß-karotina 6,1 µg g-1, hlorofila 4,9 µg g-1). Sadržaj ß-karotina i hlorofila, nakon testa ubrzanog starenja smanjio se za 42,8% odnosno 60,5%, dok je sadržaj tokoferol α ostao nepromenjen. Sadržaj tokoferol α ostao je nepromenjen i u semenu sorti Williams 82 i L93-7290 nakon ubrzanog starenja. Nakon testa ubrzanog starenja u semenu sorte Williams 82 sadržaj ß-karotina je ostao isti, dok je u semenu sorte L93-7290 smanjen za 7,7%. S obzirom da nakon testa ubrzanog starenja nije došlo do promene sadržaja masnih kiselina kod sorte Goyou Kurakake, može se zaključiti da antioksidacioni sistem ima značajnu ulogu u zaštiti od lipidne peroksidacije.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Effect of multiple alleles on oxidative stability and germination of soybean seeds subsequent to the accelerated ageing test
T1  - Uticaj multiplih alela na oksidacionu stabilnost i klijavost semena soje nakon ubrzanog starenja
VL  - 38
IS  - 1
SP  - 37
EP  - 48
DO  - 10.2298/GENSR0601037Z
UR  - conv_398
ER  - 
@article{
author = "Žilić, Slađana and Milivojević, Marija and Šobajić, Slađana and Maksimović, Milan",
year = "2006",
abstract = "The purpose of this study was to determine changes of lipoxygenase activities, contents of antioxidants (tocopherol α, β-carotene and chlorophyll) fatty acids and soluble proteins, as well as, vigor in accelerated aged soybean seeds. The following soybean cultivars were used in the study: Williams 82, Goyou Kurakake and L93-7290. Subsequent to the accelerated ageing test (AAT), 23% of seeds of the cultivar Goyou Kurakake retained normal germination, while grain of the two remaining cultivars L93-7290 and Williams 82, completely lost germination ability. According to our results, the seeds of the cultivar Goyou Kurakake (the absence of lipoxygenases 2) is characterized by a significantly higher content of all observed antioxidants (tocopherol α 2.7 mg 100g-1, β-carotene 6.1 µg g-1 chlorophyll 4.9 µg g-1). The contents of (β-carotene and chlorophyll decreased after AAT in the seed of the cultivar Goyou Kurakake by 42.8% and 60.5%, respectively, while the content of tocopherol a remained the same. Furthermore, the tocopherol a content was not changed after AAT neither in the cultivar L93-7290 nor in the cultivar Williams 82. The content of β-carotene after AAT remained the same in seeds of the cultivar Williams 82 while it decreased by 7.7% in the cultivar L93-7290. Since changes of the fatty acids content were not pronounced in the cultivar Goyou Kurakake after AAT it can be concluded that the antioxidative system had a crucial role in seeds protection against lipid peroxidation., Svrha ovih istraživanja bila je da se odrede promene aktivnosti izoenzima lipoksigenaze, sadržaja antioksidanasa (tokoferola α, ß-karotina i hlorofila), masnih kiselina i rastvorljivih proteina, kao i životne sposobnosti semena soje nakon ubrzanog starenja. Za istraživanja su korišćene sledeće sorte soje: Williams 82, Goyou Kurakake and L93-7290. Nakon testa ubrzanog starenja 23% semena sorte Goyou Kurakake zadržalo je normalnu klijavost, dok je seme sorti Williams 82 i L93-7290 potpuno izgubilo klijavost. Na osnovu naših rezultata sorta Goyou Kurakake, koja se odlikuje odsustvom lipoksigenaze 2, imala je značajno viši sadržaj ispitivanih antioksidanasa (tokofherol α 2,7 mg 100g-1, ß-karotina 6,1 µg g-1, hlorofila 4,9 µg g-1). Sadržaj ß-karotina i hlorofila, nakon testa ubrzanog starenja smanjio se za 42,8% odnosno 60,5%, dok je sadržaj tokoferol α ostao nepromenjen. Sadržaj tokoferol α ostao je nepromenjen i u semenu sorti Williams 82 i L93-7290 nakon ubrzanog starenja. Nakon testa ubrzanog starenja u semenu sorte Williams 82 sadržaj ß-karotina je ostao isti, dok je u semenu sorte L93-7290 smanjen za 7,7%. S obzirom da nakon testa ubrzanog starenja nije došlo do promene sadržaja masnih kiselina kod sorte Goyou Kurakake, može se zaključiti da antioksidacioni sistem ima značajnu ulogu u zaštiti od lipidne peroksidacije.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Effect of multiple alleles on oxidative stability and germination of soybean seeds subsequent to the accelerated ageing test, Uticaj multiplih alela na oksidacionu stabilnost i klijavost semena soje nakon ubrzanog starenja",
volume = "38",
number = "1",
pages = "37-48",
doi = "10.2298/GENSR0601037Z",
url = "conv_398"
}
Žilić, S., Milivojević, M., Šobajić, S.,& Maksimović, M.. (2006). Effect of multiple alleles on oxidative stability and germination of soybean seeds subsequent to the accelerated ageing test. in Genetika
Društvo genetičara Srbije, Beograd., 38(1), 37-48.
https://doi.org/10.2298/GENSR0601037Z
conv_398
Žilić S, Milivojević M, Šobajić S, Maksimović M. Effect of multiple alleles on oxidative stability and germination of soybean seeds subsequent to the accelerated ageing test. in Genetika. 2006;38(1):37-48.
doi:10.2298/GENSR0601037Z
conv_398 .
Žilić, Slađana, Milivojević, Marija, Šobajić, Slađana, Maksimović, Milan, "Effect of multiple alleles on oxidative stability and germination of soybean seeds subsequent to the accelerated ageing test" in Genetika, 38, no. 1 (2006):37-48,
https://doi.org/10.2298/GENSR0601037Z .,
conv_398 .
4