Obradović, Ana

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orcid::0000-0003-4492-1576
  • Obradović, Ana (12)
  • Stepanić, Ana (4)

Author's Bibliography

First report of Aspergillus parasiticus on Barley Grain in Serbia

Nikolić, Milica; Savić, Iva; Obradović, Ana; Srdić, Jelena; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(St. Paul : The American Phytopathological Society (APS), 2020)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Obradović, Ana
AU  - Srdić, Jelena
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/796
AB  - Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.
PB  - St. Paul : The American Phytopathological Society (APS)
T2  - Plant Disease
T1  - First report of Aspergillus parasiticus on Barley Grain in Serbia
VL  - 104
IS  - 3
SP  - 987
DO  - 10.1094/PDIS-07-19-1364-PDN
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Obradović, Ana and Srdić, Jelena and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2020",
url = "http://rik.mrizp.rs/handle/123456789/796",
abstract = "Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.",
publisher = "St. Paul : The American Phytopathological Society (APS)",
journal = "Plant Disease",
title = "First report of Aspergillus parasiticus on Barley Grain in Serbia",
volume = "104",
number = "3",
pages = "987",
doi = "10.1094/PDIS-07-19-1364-PDN"
}
Nikolić, M., Savić, I., Obradović, A., Srdić, J., Stanković, G., Stevanović, M.,& Stanković, S. (2020). First report of Aspergillus parasiticus on Barley Grain in Serbia.
Plant Disease
St. Paul : The American Phytopathological Society (APS)., 104(3), 987.
https://doi.org/10.1094/PDIS-07-19-1364-PDN
Nikolić M, Savić I, Obradović A, Srdić J, Stanković G, Stevanović M, Stanković S. First report of Aspergillus parasiticus on Barley Grain in Serbia. Plant Disease. 2020;104(3):987
Nikolić Milica, Savić Iva, Obradović Ana, Srdić Jelena, Stanković Goran, Stevanović Milan, Stanković Slavica, "First report of Aspergillus parasiticus on Barley Grain in Serbia" Plant Disease, 104, no. 3 (2020):987,
https://doi.org/10.1094/PDIS-07-19-1364-PDN .

Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains

Krnjaja, Vesna; Mandić, Violeta; Lukić, Miloš; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Vasić, Tanja
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/735
AB  - Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains
VL  - 116
SP  - 126
EP  - 131
DO  - 10.1016/j.cropro.2018.10.021
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lukić, Miloš and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Vasić, Tanja",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/735",
abstract = "Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains",
volume = "116",
pages = "126-131",
doi = "10.1016/j.cropro.2018.10.021"
}
Krnjaja, V., Mandić, V., Lukić, M., Bijelić, Z., Stanković, S., Obradović, A.,& Vasić, T. (2019). Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains.
Crop Protection
Elsevier Sci Ltd, Oxford., 116, 126-131.
https://doi.org/10.1016/j.cropro.2018.10.021
Krnjaja V, Mandić V, Lukić M, Bijelić Z, Stanković S, Obradović A, Vasić T. Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains. Crop Protection. 2019;116:126-131
Krnjaja Vesna, Mandić Violeta, Lukić Miloš, Bijelić Zorica, Stanković Slavica, Obradović Ana, Vasić Tanja, "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains" Crop Protection, 116 (2019):126-131,
https://doi.org/10.1016/j.cropro.2018.10.021 .
7
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Impact of climatic conditions on fumonisins in maize grown in Serbia

Udovicki, B.; Đekić, I.; Rajković, A.; Stanković, Slavica; Obradović, Ana

(Wageningen Academic Publishers, Wageningen, 2019)

TY  - JOUR
AU  - Udovicki, B.
AU  - Đekić, I.
AU  - Rajković, A.
AU  - Stanković, Slavica
AU  - Obradović, Ana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/737
AB  - The influence of climatic conditions on the levels of fumonisins in maize grown in Serbia was studied over eight years (2008 to 2015), investigating the possible relationship between the levels of fumonisins contamination in maize in relation to registered weather/climatic conditions. Presence of these mycotoxins in maize after harvest was evaluated based on climatic conditions within two periods: 10 days before and 10 days after 50% silking. Categories of fumonisins levels were transformed into classes. Chi-square test for association was used in analysing relationships between results of fumonisins levels and calendar years. Mann-Whitney U-test was used to compare differences between meteorological data of two subsets in years with high and low fumonisins level. There was a statistically significant association between the fumonisins levels and calendar years chi(2)=247.954; (P lt 0.05). This study identified low precipitation and low humidity combined with high solar radiation as a critical parameter combination for elevated levels of fumonisins. The statistically significant difference in relation to some of the examined parameters suggests that maize in Serbian agro-climatic conditions is more susceptible to fungal colonisation, and subsequent fumonisin production in the period of 10 days before 50% silking.
PB  - Wageningen Academic Publishers, Wageningen
T2  - World Mycotoxin Journal
T1  - Impact of climatic conditions on fumonisins in maize grown in Serbia
VL  - 12
IS  - 2
SP  - 183
EP  - 190
DO  - 10.3920/WMJ2018.2364
ER  - 
@article{
author = "Udovicki, B. and Đekić, I. and Rajković, A. and Stanković, Slavica and Obradović, Ana",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/737",
abstract = "The influence of climatic conditions on the levels of fumonisins in maize grown in Serbia was studied over eight years (2008 to 2015), investigating the possible relationship between the levels of fumonisins contamination in maize in relation to registered weather/climatic conditions. Presence of these mycotoxins in maize after harvest was evaluated based on climatic conditions within two periods: 10 days before and 10 days after 50% silking. Categories of fumonisins levels were transformed into classes. Chi-square test for association was used in analysing relationships between results of fumonisins levels and calendar years. Mann-Whitney U-test was used to compare differences between meteorological data of two subsets in years with high and low fumonisins level. There was a statistically significant association between the fumonisins levels and calendar years chi(2)=247.954; (P lt 0.05). This study identified low precipitation and low humidity combined with high solar radiation as a critical parameter combination for elevated levels of fumonisins. The statistically significant difference in relation to some of the examined parameters suggests that maize in Serbian agro-climatic conditions is more susceptible to fungal colonisation, and subsequent fumonisin production in the period of 10 days before 50% silking.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "World Mycotoxin Journal",
title = "Impact of climatic conditions on fumonisins in maize grown in Serbia",
volume = "12",
number = "2",
pages = "183-190",
doi = "10.3920/WMJ2018.2364"
}
Udovicki, B., Đekić, I., Rajković, A., Stanković, S.,& Obradović, A. (2019). Impact of climatic conditions on fumonisins in maize grown in Serbia.
World Mycotoxin Journal
Wageningen Academic Publishers, Wageningen., 12(2), 183-190.
https://doi.org/10.3920/WMJ2018.2364
Udovicki B, Đekić I, Rajković A, Stanković S, Obradović A. Impact of climatic conditions on fumonisins in maize grown in Serbia. World Mycotoxin Journal. 2019;12(2):183-190
Udovicki B., Đekić I., Rajković A., Stanković Slavica, Obradović Ana, "Impact of climatic conditions on fumonisins in maize grown in Serbia" World Mycotoxin Journal, 12, no. 2 (2019):183-190,
https://doi.org/10.3920/WMJ2018.2364 .
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Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji

Obradović, Ana

(Универзитет у Београду, Пољопривредни факултет, 2018)

TY  - BOOK
AU  - Obradović, Ana
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6037
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:18357/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50361615
UR  - http://nardus.mpn.gov.rs/123456789/10079
UR  - http://rik.mrizp.rs/handle/123456789/756
AB  - Za proučavanje diverziteta vrste F. graminearum odabran je 101 izolat izkolekcije Laboratorije za fitopatologiju Instituta za kukuruz "Zemun Polje" koji suprethodno identifikovani konvencionalnim metodama na osnovu makroskopskih imikroskopskih svojstava. Izolati su prikupljeni u periodu od 1993. do 2015. godine, aporeklom iz uzoraka pšenice (42), kukuruza (37) i ječma (22) iz 41 lokaliteta nateritoriji Srbije. Sa ciljem da se dobije uvid o rasprostranjenosti, prisustvu i diverzitetuvrsta Fusarium graminearum kompleksa (Fg) kao i o trihotecenskim hemotipovima uSrbiji, obavljena su proučavanja morfoloških, patogenih, toksigenih i filogenetskihsvojstava odabranih izolata. Na osnovu svih proučenih svojstava utvrđeno je prisustvotri vrste u okviru Fg kompleksa: Fusarium graminearum sensu stricto, Fusariumboothii i Fusarium vorosii. Po prvi put u Srbiji, detektovano je prisustvo F. boothii i F.vorosii patogena strnih žita i kukuruza u našoj zemlji.Na osnovu morfoloških, patogenih i toksigenih svojstava uočen je velikidiverzitet ispitivanih izolata. Rezultati analize morfoloških svojstava izolata F.graminearum ss, F. boothii i F. vorosii, ukazuju da ne postoje razlike među njima.Izolati vrste F. boothii i F. vorosii ispoljili su generalno slabiju patogenost u poređenjusa ostalim izolatima. Analiza potencijala produkcije mikotoksina deoksinivalenola izearalenona ELISA i HPLC metodom ukazala je na varijabilnost između ispitivanihizolata. Određivanjem trihotecenskih hemotipova hemijskom analizom utvrđeno je da uSrbiji postoje dva hemotipa - 15ADON (dominantan) i 3ADON.Za razliku od hemijskih, molekularnim analizama na osnovu TRI3 i TRI12 gena,utvrđeno je prisustvo samo 15ADON hemotipa. Dobijeni rezultati o biodiverzitetuhemotipova su prvi nalaz u agroekološkim uslovima Srbije. Analiza nukleotidnihsekvenci tri genomska regiona (TEF−1α, β−tubulin i histon H3), pokazala je da postojerazličiti potencijali za razdvajanje vrsta u okviru Fg kompleksa. Rezultati ove disertacijeukazali su na postojanje dve vrste u okviru Fg kompleksa (F. boothii i F. vorosii), kojedo sada nisu identifikovane na teritoriji Srbije. Sekvenciranje ovih genomskih regionaodabranih izolata i njihovo poređenje sa referentnim izolatima kao i poređenje saizolatima Fg kompleksa u svetu doprinelo je boljem poznavanju biodiverziteta ovihvrsta u Srbiji.
AB  - One hundred and one isolates of the F. graminearum species have been isolatedfrom the collection of the Laboratory of Phytopathology of the Maize ResearchInstitute, Zemun Polje to study the species diversity. These isolates had been previouslyidentified using conventional methods based on macroscopic and microscopicproperties. Isolates, originating from samples of wheat (42), maize (37) and barley (22),were collected from 41 locations in Serbia in the period from 1993 to 2015. In order togain insight into the distribution, presence and diversity of the Fusarium graminearumspecies complex (Fg), as well as into thrichotecene chemotypes in Serbia, studies of themorphological, pathogenic, toxigenic and phylogenetic properties of the selectedisolates were carried out. According to all observed properties, the presence of the threefollowing species was determined within the Fg complex: Fusarium graminearumsensu stricto, Fusarium boothii and Fusarium vorosii. F. boothii and F. vorosii,pathogens of small grains and maize in our country, were detected for the first time inSerbia.Based on morphological, pathogenic and toxigenic properties, a great diversityof analysed isolates was observed. Results of analyses of morphological properties ofthe isolates of F. graminearum ss, F. boothii and F. vorosii, pointed out that there hadbeen no differences among them. Isolates of F. boothii and F. vorosii species exhibitedgenerally less pathogenicity than remaining isolates. The analysis of the potential for theproduction of mycotoxins deoxynivalenol and zearalenone by the ELISA and the HPLCmethod pointed out to the variability among analysed isolates. The determination oftrichothecene chemotypes done by chemical analyses confirmed the presence of twochemotypes in Serbia- 15ADON (dominant) and 3ADON. In contrast to chemicalanalyses, the molecular analyses, based on the TRI3 and TRI12 genes, established thepresence of only 15ADON chemotype. The obtained results on the biodiversity ofchemotypes are the first finding under agroecological conditions of Serbia. The analysisof nucleotide sequences of the three genomic regions (TEF−1α, β−tubulin and histoneH3), showed the existence of different potentials for separation of species within the Fgcomplex. The results presented in this PhD thesis point out to the existence of twospecies (F. boothii and F. vorosii) within the Fg complex, which have not yet beenidentified in the territory of Serbia. Sequencing these portions of the genome of theselected isolates and their comparison with the reference isolates, as well as, thecomparison with the Fg complex isolates in the world contributed to a better knowledgeof the biodiversity of these species in Serbia.
PB  - Универзитет у Београду, Пољопривредни факултет
T2  - Универзитет у Београду
T1  - Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji
ER  - 
@phdthesis{
author = "Obradović, Ana",
year = "2018",
url = "http://eteze.bg.ac.rs/application/showtheses?thesesId=6037, https://fedorabg.bg.ac.rs/fedora/get/o:18357/bdef:Content/download, http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50361615, http://nardus.mpn.gov.rs/123456789/10079, http://rik.mrizp.rs/handle/123456789/756",
abstract = "Za proučavanje diverziteta vrste F. graminearum odabran je 101 izolat izkolekcije Laboratorije za fitopatologiju Instituta za kukuruz "Zemun Polje" koji suprethodno identifikovani konvencionalnim metodama na osnovu makroskopskih imikroskopskih svojstava. Izolati su prikupljeni u periodu od 1993. do 2015. godine, aporeklom iz uzoraka pšenice (42), kukuruza (37) i ječma (22) iz 41 lokaliteta nateritoriji Srbije. Sa ciljem da se dobije uvid o rasprostranjenosti, prisustvu i diverzitetuvrsta Fusarium graminearum kompleksa (Fg) kao i o trihotecenskim hemotipovima uSrbiji, obavljena su proučavanja morfoloških, patogenih, toksigenih i filogenetskihsvojstava odabranih izolata. Na osnovu svih proučenih svojstava utvrđeno je prisustvotri vrste u okviru Fg kompleksa: Fusarium graminearum sensu stricto, Fusariumboothii i Fusarium vorosii. Po prvi put u Srbiji, detektovano je prisustvo F. boothii i F.vorosii patogena strnih žita i kukuruza u našoj zemlji.Na osnovu morfoloških, patogenih i toksigenih svojstava uočen je velikidiverzitet ispitivanih izolata. Rezultati analize morfoloških svojstava izolata F.graminearum ss, F. boothii i F. vorosii, ukazuju da ne postoje razlike među njima.Izolati vrste F. boothii i F. vorosii ispoljili su generalno slabiju patogenost u poređenjusa ostalim izolatima. Analiza potencijala produkcije mikotoksina deoksinivalenola izearalenona ELISA i HPLC metodom ukazala je na varijabilnost između ispitivanihizolata. Određivanjem trihotecenskih hemotipova hemijskom analizom utvrđeno je da uSrbiji postoje dva hemotipa - 15ADON (dominantan) i 3ADON.Za razliku od hemijskih, molekularnim analizama na osnovu TRI3 i TRI12 gena,utvrđeno je prisustvo samo 15ADON hemotipa. Dobijeni rezultati o biodiverzitetuhemotipova su prvi nalaz u agroekološkim uslovima Srbije. Analiza nukleotidnihsekvenci tri genomska regiona (TEF−1α, β−tubulin i histon H3), pokazala je da postojerazličiti potencijali za razdvajanje vrsta u okviru Fg kompleksa. Rezultati ove disertacijeukazali su na postojanje dve vrste u okviru Fg kompleksa (F. boothii i F. vorosii), kojedo sada nisu identifikovane na teritoriji Srbije. Sekvenciranje ovih genomskih regionaodabranih izolata i njihovo poređenje sa referentnim izolatima kao i poređenje saizolatima Fg kompleksa u svetu doprinelo je boljem poznavanju biodiverziteta ovihvrsta u Srbiji., One hundred and one isolates of the F. graminearum species have been isolatedfrom the collection of the Laboratory of Phytopathology of the Maize ResearchInstitute, Zemun Polje to study the species diversity. These isolates had been previouslyidentified using conventional methods based on macroscopic and microscopicproperties. Isolates, originating from samples of wheat (42), maize (37) and barley (22),were collected from 41 locations in Serbia in the period from 1993 to 2015. In order togain insight into the distribution, presence and diversity of the Fusarium graminearumspecies complex (Fg), as well as into thrichotecene chemotypes in Serbia, studies of themorphological, pathogenic, toxigenic and phylogenetic properties of the selectedisolates were carried out. According to all observed properties, the presence of the threefollowing species was determined within the Fg complex: Fusarium graminearumsensu stricto, Fusarium boothii and Fusarium vorosii. F. boothii and F. vorosii,pathogens of small grains and maize in our country, were detected for the first time inSerbia.Based on morphological, pathogenic and toxigenic properties, a great diversityof analysed isolates was observed. Results of analyses of morphological properties ofthe isolates of F. graminearum ss, F. boothii and F. vorosii, pointed out that there hadbeen no differences among them. Isolates of F. boothii and F. vorosii species exhibitedgenerally less pathogenicity than remaining isolates. The analysis of the potential for theproduction of mycotoxins deoxynivalenol and zearalenone by the ELISA and the HPLCmethod pointed out to the variability among analysed isolates. The determination oftrichothecene chemotypes done by chemical analyses confirmed the presence of twochemotypes in Serbia- 15ADON (dominant) and 3ADON. In contrast to chemicalanalyses, the molecular analyses, based on the TRI3 and TRI12 genes, established thepresence of only 15ADON chemotype. The obtained results on the biodiversity ofchemotypes are the first finding under agroecological conditions of Serbia. The analysisof nucleotide sequences of the three genomic regions (TEF−1α, β−tubulin and histoneH3), showed the existence of different potentials for separation of species within the Fgcomplex. The results presented in this PhD thesis point out to the existence of twospecies (F. boothii and F. vorosii) within the Fg complex, which have not yet beenidentified in the territory of Serbia. Sequencing these portions of the genome of theselected isolates and their comparison with the reference isolates, as well as, thecomparison with the Fg complex isolates in the world contributed to a better knowledgeof the biodiversity of these species in Serbia.",
publisher = "Универзитет у Београду, Пољопривредни факултет",
journal = "Универзитет у Београду",
title = "Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji"
}
Obradović, A. (2018). Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji.
Универзитет у Београду
Универзитет у Београду, Пољопривредни факултет..
Obradović A. Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji. Универзитет у Београду. 2018;
Obradović Ana, "Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji" Универзитет у Београду (2018)

Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia

Krnjaja, Vesna; Mandić, Violeta; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Bozić, Manja

(Mdpi, Basel, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Bozić, Manja
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/702
AB  - Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.
PB  - Mdpi, Basel
T2  - Toxins
T1  - Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia
VL  - 10
IS  - 11
DO  - 10.3390/toxins10110460
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Bozić, Manja",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/702",
abstract = "Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.",
publisher = "Mdpi, Basel",
journal = "Toxins",
title = "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia",
volume = "10",
number = "11",
doi = "10.3390/toxins10110460"
}
Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T.,& Bozić, M. (2018). Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia.
Toxins
Mdpi, Basel., 10(11).
https://doi.org/10.3390/toxins10110460
Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Bozić M. Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. Toxins. 2018;10(11)
Krnjaja Vesna, Mandić Violeta, Bijelić Zorica, Stanković Slavica, Obradović Ana, Petrović Tanja, Bozić Manja, "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia" Toxins, 10, no. 11 (2018),
https://doi.org/10.3390/toxins10110460 .
3
1
2

Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence

Obradović, Ana; Krnjaja, Vesna; Nikolić, Milica; Delibašić, Goran; Filipović, Milomir; Stanković, Goran; Stanković, Slavica

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Nikolić, Milica
AU  - Delibašić, Goran
AU  - Filipović, Milomir
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/738
AB  - Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species.
AB  - Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence
T1  - Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava
VL  - 34
IS  - 4
SP  - 469
EP  - 480
DO  - 10.2298/BAH1804469O
ER  - 
@article{
author = "Obradović, Ana and Krnjaja, Vesna and Nikolić, Milica and Delibašić, Goran and Filipović, Milomir and Stanković, Goran and Stanković, Slavica",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/738",
abstract = "Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species., Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence, Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava",
volume = "34",
number = "4",
pages = "469-480",
doi = "10.2298/BAH1804469O"
}
Obradović, A., Krnjaja, V., Nikolić, M., Delibašić, G., Filipović, M., Stanković, G.,& Stanković, S. (2018). Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(4), 469-480.
https://doi.org/10.2298/BAH1804469O
Obradović A, Krnjaja V, Nikolić M, Delibašić G, Filipović M, Stanković G, Stanković S. Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava. Biotechnology in Animal Husbandry. 2018;34(4):469-480
Obradović Ana, Krnjaja Vesna, Nikolić Milica, Delibašić Goran, Filipović Milomir, Stanković Goran, Stanković Slavica, "Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava" Biotechnology in Animal Husbandry, 34, no. 4 (2018):469-480,
https://doi.org/10.2298/BAH1804469O .
4

Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia

Obradović, Ana; Stanković, Slavica; Nikolić, Ana; Ignjatović-Micić, Dragana; Krnjaja, Vesna; Stepanović, Jelena; Duduk, Bojan

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Krnjaja, Vesna
AU  - Stepanović, Jelena
AU  - Duduk, Bojan
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/682
AB  - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia
VL  - 49
IS  - 1
SP  - 355
EP  - 364
DO  - 10.2298/GENSR1701355O
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Nikolić, Ana and Ignjatović-Micić, Dragana and Krnjaja, Vesna and Stepanović, Jelena and Duduk, Bojan",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/682",
abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia",
volume = "49",
number = "1",
pages = "355-364",
doi = "10.2298/GENSR1701355O"
}
Obradović, A., Stanković, S., Nikolić, A., Ignjatović-Micić, D., Krnjaja, V., Stepanović, J.,& Duduk, B. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia.
Genetika
Društvo genetičara Srbije, Beograd., 49(1), 355-364.
https://doi.org/10.2298/GENSR1701355O
Obradović A, Stanković S, Nikolić A, Ignjatović-Micić D, Krnjaja V, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. Genetika. 2017;49(1):355-364
Obradović Ana, Stanković Slavica, Nikolić Ana, Ignjatović-Micić Dragana, Krnjaja Vesna, Stepanović Jelena, Duduk Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" Genetika, 49, no. 1 (2017):355-364,
https://doi.org/10.2298/GENSR1701355O .
3
3
3

Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia

Obradović, Ana; Stanković, Slavica; Stevanović, Milan; Krnjaja, Vesna; Bulajić, Aleksandra; Delibašić, Goran

(Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Stevanović, Milan
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Delibašić, Goran
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/665
AB  - Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum.
AB  - Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia
T1  - Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji
VL  - 45
IS  - 3
SP  - 277
EP  - 286
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Stevanović, Milan and Krnjaja, Vesna and Bulajić, Aleksandra and Delibašić, Goran",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/665",
abstract = "Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum., Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia, Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji",
volume = "45",
number = "3",
pages = "277-286"
}
Obradović, A., Stanković, S., Stevanović, M., Krnjaja, V., Bulajić, A.,& Delibašić, G. (2017). Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji.
Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(3), 277-286.
Obradović A, Stanković S, Stevanović M, Krnjaja V, Bulajić A, Delibašić G. Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji. Biljni lekar. 2017;45(3):277-286
Obradović Ana, Stanković Slavica, Stevanović Milan, Krnjaja Vesna, Bulajić Aleksandra, Delibašić Goran, "Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji" Biljni lekar, 45, no. 3 (2017):277-286

Fungal and mycotoxin contamination of maize hybrids in different maturity groups

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Bijelić, Zorica; Vasić, Tanja; Jauković, Marko

(Institut za stočarstvo, Beograd, 2016)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Vasić, Tanja
AU  - Jauković, Marko
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/627
AB  - In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods.
AB  - U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal and mycotoxin contamination of maize hybrids in different maturity groups
T1  - Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja
VL  - 32
IS  - 1
SP  - 71
EP  - 81
DO  - 10.2298/BAH1601071K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Vasić, Tanja and Jauković, Marko",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/627",
abstract = "In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods., U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal and mycotoxin contamination of maize hybrids in different maturity groups, Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja",
volume = "32",
number = "1",
pages = "71-81",
doi = "10.2298/BAH1601071K"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Bijelić, Z., Vasić, T.,& Jauković, M. (2016). Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(1), 71-81.
https://doi.org/10.2298/BAH1601071K
Krnjaja V, Stanković S, Obradović A, Mandić V, Bijelić Z, Vasić T, Jauković M. Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja. Biotechnology in Animal Husbandry. 2016;32(1):71-81
Krnjaja Vesna, Stanković Slavica, Obradović Ana, Mandić Violeta, Bijelić Zorica, Vasić Tanja, Jauković Marko, "Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja" Biotechnology in Animal Husbandry, 32, no. 1 (2016):71-81,
https://doi.org/10.2298/BAH1601071K .
4

Fusarium infection and deoxynivalenol contamination in winter wheat

Krnjaja, Vesna; Tomić, Z.; Stanković, Slavica; Petrović, T.; Bijelić, Zorica; Mandić, Violeta; Obradović, Ana

(Institut za stočarstvo, Beograd, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Tomić, Z.
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Obradović, Ana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/606
AB  - In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat.
AB  - U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fusarium infection and deoxynivalenol contamination in winter wheat
T1  - Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice
VL  - 31
IS  - 1
SP  - 123
EP  - 131
DO  - 10.2298/BAH1501123K
ER  - 
@article{
author = "Krnjaja, Vesna and Tomić, Z. and Stanković, Slavica and Petrović, T. and Bijelić, Zorica and Mandić, Violeta and Obradović, Ana",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/606",
abstract = "In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat., U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fusarium infection and deoxynivalenol contamination in winter wheat, Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice",
volume = "31",
number = "1",
pages = "123-131",
doi = "10.2298/BAH1501123K"
}
Krnjaja, V., Tomić, Z., Stanković, S., Petrović, T., Bijelić, Z., Mandić, V.,& Obradović, A. (2015). Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 31(1), 123-131.
https://doi.org/10.2298/BAH1501123K
Krnjaja V, Tomić Z, Stanković S, Petrović T, Bijelić Z, Mandić V, Obradović A. Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice. Biotechnology in Animal Husbandry. 2015;31(1):123-131
Krnjaja Vesna, Tomić Z., Stanković Slavica, Petrović T., Bijelić Zorica, Mandić Violeta, Obradović Ana, "Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice" Biotechnology in Animal Husbandry, 31, no. 1 (2015):123-131,
https://doi.org/10.2298/BAH1501123K .
5

Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat

Krnjaja, Vesna; Mandić, Violeta; Lević, Jelena; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/602
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
VL  - 67
SP  - 251
EP  - 256
DO  - 10.1016/j.cropro.2014.11.001
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/602",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
volume = "67",
pages = "251-256",
doi = "10.1016/j.cropro.2014.11.001"
}
Krnjaja, V., Mandić, V., Lević, J., Stanković, S., Obradović, A., Petrović, T.,& Vasić, T. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat.
Crop Protection
Elsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001
Krnjaja V, Mandić V, Lević J, Stanković S, Obradović A, Petrović T, Vasić T. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. Crop Protection. 2015;67:251-256
Krnjaja Vesna, Mandić Violeta, Lević Jelena, Stanković Slavica, Obradović Ana, Petrović Tanja, Vasić Tanja, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" Crop Protection, 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 .
15
8
13

Presence of deoxynivalenol in winter wheat treated with fungicides

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Mandić, Violeta; Tomić, Z.; Obradović, Ana

(Institut za stočarstvo, Beograd, 2014)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Mandić, Violeta
AU  - Tomić, Z.
AU  - Obradović, Ana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/566
AB  - Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively).
AB  - U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Presence of deoxynivalenol in winter wheat treated with fungicides
T1  - Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima
VL  - 30
IS  - 1
SP  - 167
EP  - 173
DO  - 10.2298/BAH1401167K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Mandić, Violeta and Tomić, Z. and Obradović, Ana",
year = "2014",
url = "http://rik.mrizp.rs/handle/123456789/566",
abstract = "Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively)., U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Presence of deoxynivalenol in winter wheat treated with fungicides, Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima",
volume = "30",
number = "1",
pages = "167-173",
doi = "10.2298/BAH1401167K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Mandić, V., Tomić, Z.,& Obradović, A. (2014). Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 30(1), 167-173.
https://doi.org/10.2298/BAH1401167K
Krnjaja V, Lević J, Stanković S, Petrović T, Mandić V, Tomić Z, Obradović A. Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima. Biotechnology in Animal Husbandry. 2014;30(1):167-173
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Mandić Violeta, Tomić Z., Obradović Ana, "Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima" Biotechnology in Animal Husbandry, 30, no. 1 (2014):167-173,
https://doi.org/10.2298/BAH1401167K .
1

An outbreak of Aspergillus species in response to environmental conditions in Serbia

Lević, Jelena; Gošić-Dondo, Snežana; Ivanović, Dragica; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra; Stepanić, Ana

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Gošić-Dondo, Snežana
AU  - Ivanović, Dragica
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/510
AB  - The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low.
AB  - U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - An outbreak of Aspergillus species in response to environmental conditions in Serbia
T1  - Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji
VL  - 28
IS  - 3
SP  - 167
EP  - 179
DO  - 10.2298/PIF1303167L
ER  - 
@article{
author = "Lević, Jelena and Gošić-Dondo, Snežana and Ivanović, Dragica and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra and Stepanić, Ana",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/510",
abstract = "The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low., U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "An outbreak of Aspergillus species in response to environmental conditions in Serbia, Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji",
volume = "28",
number = "3",
pages = "167-179",
doi = "10.2298/PIF1303167L"
}
Lević, J., Gošić-Dondo, S., Ivanović, D., Stanković, S., Krnjaja, V., Bočarov-Stančić, A.,& Stepanić, A. (2013). Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji.
Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(3), 167-179.
https://doi.org/10.2298/PIF1303167L
Lević J, Gošić-Dondo S, Ivanović D, Stanković S, Krnjaja V, Bočarov-Stančić A, Stepanić A. Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji. Pesticidi i fitomedicina. 2013;28(3):167-179
Lević Jelena, Gošić-Dondo Snežana, Ivanović Dragica, Stanković Slavica, Krnjaja Vesna, Bočarov-Stančić Aleksandra, Stepanić Ana, "Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji" Pesticidi i fitomedicina, 28, no. 3 (2013):167-179,
https://doi.org/10.2298/PIF1303167L .
29

Detection of Rhizomucor pusillus on sunflower seed

Lević, Jelena; Ivanović, D.; Stanković, Slavica; Milivojević, Marija; Vukadinović, Radmila; Stepanić, Ana

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Ivanović, D.
AU  - Stanković, Slavica
AU  - Milivojević, Marija
AU  - Vukadinović, Radmila
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/478
AB  - The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - Detection of Rhizomucor pusillus on sunflower seed
VL  - 36
IS  - 59
SP  - 59
EP  - 70
DO  - 10.2298/HEL1359059L
ER  - 
@article{
author = "Lević, Jelena and Ivanović, D. and Stanković, Slavica and Milivojević, Marija and Vukadinović, Radmila and Stepanić, Ana",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/478",
abstract = "The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Detection of Rhizomucor pusillus on sunflower seed",
volume = "36",
number = "59",
pages = "59-70",
doi = "10.2298/HEL1359059L"
}
Lević, J., Ivanović, D., Stanković, S., Milivojević, M., Vukadinović, R.,& Stepanić, A. (2013). Detection of Rhizomucor pusillus on sunflower seed.
Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 36(59), 59-70.
https://doi.org/10.2298/HEL1359059L
Lević J, Ivanović D, Stanković S, Milivojević M, Vukadinović R, Stepanić A. Detection of Rhizomucor pusillus on sunflower seed. Helia. 2013;36(59):59-70
Lević Jelena, Ivanović D., Stanković Slavica, Milivojević Marija, Vukadinović Radmila, Stepanić Ana, "Detection of Rhizomucor pusillus on sunflower seed" Helia, 36, no. 59 (2013):59-70,
https://doi.org/10.2298/HEL1359059L .

Fusariotoxins in wheat grain in Serbia

Stepanić, Ana; Stanković, Slavica; Lević, Jelena; Ivanović, Mirko; Krnjaja, Vesna

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2011)

TY  - JOUR
AU  - Stepanić, Ana
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Ivanović, Mirko
AU  - Krnjaja, Vesna
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/399
AB  - Samples of wheat grain (41), collected during the 2010 harvest from seven localities in Serbia, were analyzed for the presence of zearalenone (ZEA), T-2 toxin, deoxynivalenol (DON) and fumonisine B1 (FB1). Results of Enzyme-Linked Immunosorbent Assay (ELISA) showed that all analysed samples were positive for the presence of at least one of four observed fusariotoxins. The most distributed mycotoxins were ZEA (90.2%, with the average concentration of 442.6μg kg-1) and T-2 (90.2%, with the average concentration of 24.2 μg kg-1). DON (73.2%) and FB1 (84.4%) were detected in a somewhat smaller number of samples, but their average concentrations were higher (1988.1 μg DON kg-1 and 882.7 μg FB1 kg-1). The established correlations between concentrations of DON and FB1 (r = 0.32) or DON and ZEA (r = 0.22) were not statistically significant. A negative correlation was established between concentrations of T-2 and FB1 (r= -0.24), as well as, between T-2 and DON (r = -0.36). Detected concentrations of ZEA and T-2 were bellow the level prescribed by the World Health Organisation (WHO), while concentrations of FB1 and DON detected in five that is, 17 samples, respectively, were above the permissible limit for human consumption.
AB  - Uzorci zrna pšenice (41), sakupljeni tokom žetve 2010. godine iz 7 različitih lokaliteta na teritoriji Srbije, analizirani su na prisustvo ZEA, T-2 toksina, deoksinivalenola (DON) i FB1. Primenom ELISA metode utvrđeno je da su svi analizirani uzorci bili pozitivni na prisustvo bar jednog od četiri ispitivana fuzariotoksina. Najzastupljeniji mikotoksini su bili ZEA (90,2%, prosečne koncentracije 442,6 μg kg-1) i T-2 (90,2%, prosečna koncentracija 24,2 kg-1). U nešto manjem broju uzoraka utvrđeni su DON (73,2%) i FB1 (84,4%), ali u većoj prosečnoj koncentraciji (1988,1 μg DON kg-1 i 882,7 μg FB1 kg-1). Nije utvrđena statistički značajna korelacija između koncentracije DON i FB1 (r = 0,32) ili DON i ZEA (r = 0,22). Negativna korelacija je utvrđena između koncentracije T-2 i FB1 (r = -0,24), kao i između T-2 i DON (r = -0,36). Detektovane koncentracije ZEA i T-2 bile su niže od nivoa koji propisuje Svetska zdravstvena organizacija, dok su FB1 i DON detektovani kod 5, odnosno 17 uzoraka u koncentraciji većoj od dozvoljenih u ljudskoj hrani.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Fusariotoxins in wheat grain in Serbia
T1  - Fuzariotoksini u zrnu pšenice u Srbiji
VL  - 26
IS  - 4
SP  - 317
EP  - 323
DO  - 10.2298/PIF1104317S
ER  - 
@article{
author = "Stepanić, Ana and Stanković, Slavica and Lević, Jelena and Ivanović, Mirko and Krnjaja, Vesna",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/399",
abstract = "Samples of wheat grain (41), collected during the 2010 harvest from seven localities in Serbia, were analyzed for the presence of zearalenone (ZEA), T-2 toxin, deoxynivalenol (DON) and fumonisine B1 (FB1). Results of Enzyme-Linked Immunosorbent Assay (ELISA) showed that all analysed samples were positive for the presence of at least one of four observed fusariotoxins. The most distributed mycotoxins were ZEA (90.2%, with the average concentration of 442.6μg kg-1) and T-2 (90.2%, with the average concentration of 24.2 μg kg-1). DON (73.2%) and FB1 (84.4%) were detected in a somewhat smaller number of samples, but their average concentrations were higher (1988.1 μg DON kg-1 and 882.7 μg FB1 kg-1). The established correlations between concentrations of DON and FB1 (r = 0.32) or DON and ZEA (r = 0.22) were not statistically significant. A negative correlation was established between concentrations of T-2 and FB1 (r= -0.24), as well as, between T-2 and DON (r = -0.36). Detected concentrations of ZEA and T-2 were bellow the level prescribed by the World Health Organisation (WHO), while concentrations of FB1 and DON detected in five that is, 17 samples, respectively, were above the permissible limit for human consumption., Uzorci zrna pšenice (41), sakupljeni tokom žetve 2010. godine iz 7 različitih lokaliteta na teritoriji Srbije, analizirani su na prisustvo ZEA, T-2 toksina, deoksinivalenola (DON) i FB1. Primenom ELISA metode utvrđeno je da su svi analizirani uzorci bili pozitivni na prisustvo bar jednog od četiri ispitivana fuzariotoksina. Najzastupljeniji mikotoksini su bili ZEA (90,2%, prosečne koncentracije 442,6 μg kg-1) i T-2 (90,2%, prosečna koncentracija 24,2 kg-1). U nešto manjem broju uzoraka utvrđeni su DON (73,2%) i FB1 (84,4%), ali u većoj prosečnoj koncentraciji (1988,1 μg DON kg-1 i 882,7 μg FB1 kg-1). Nije utvrđena statistički značajna korelacija između koncentracije DON i FB1 (r = 0,32) ili DON i ZEA (r = 0,22). Negativna korelacija je utvrđena između koncentracije T-2 i FB1 (r = -0,24), kao i između T-2 i DON (r = -0,36). Detektovane koncentracije ZEA i T-2 bile su niže od nivoa koji propisuje Svetska zdravstvena organizacija, dok su FB1 i DON detektovani kod 5, odnosno 17 uzoraka u koncentraciji većoj od dozvoljenih u ljudskoj hrani.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Fusariotoxins in wheat grain in Serbia, Fuzariotoksini u zrnu pšenice u Srbiji",
volume = "26",
number = "4",
pages = "317-323",
doi = "10.2298/PIF1104317S"
}
Stepanić, A., Stanković, S., Lević, J., Ivanović, M.,& Krnjaja, V. (2011). Fuzariotoksini u zrnu pšenice u Srbiji.
Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(4), 317-323.
https://doi.org/10.2298/PIF1104317S
Stepanić A, Stanković S, Lević J, Ivanović M, Krnjaja V. Fuzariotoksini u zrnu pšenice u Srbiji. Pesticidi i fitomedicina. 2011;26(4):317-323
Stepanić Ana, Stanković Slavica, Lević Jelena, Ivanović Mirko, Krnjaja Vesna, "Fuzariotoksini u zrnu pšenice u Srbiji" Pesticidi i fitomedicina, 26, no. 4 (2011):317-323,
https://doi.org/10.2298/PIF1104317S .
6

Fusarium species and their mycotoxins in wheat grain

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Stepanić, Ana

(Matica srpska, Novi Sad, 2011)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Stepanić, Ana
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/378
AB  - Incidence of fungi and concentration of mycotoxin deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the grain of the winter wheat collected subsequently after harvesting in 2010. In the analyzed samples the highest incidence was determined for the species of Fusarium (84.7%) genus, significantly lower incidence was determined for the species of genus Alternaria (12.35%), and especially for species of genera Acremoniella (2.05%), Acremonium (0.65%) and Penicillium (0.25%). F. graminearum (99.05%) was the most present species of Fusarium genus, whereas the following species F. sporotrichioides (0.4%), F. subglutinans (0.4%), F. poae (0.05%), F. proliferatum (0.05%) and F. verticillioides (0.05%) were only sporadic. The presence of DON, ZON and FB1 mycotoxins was established in all studied wheat samples. DON was detected in concentrations from 123 to 393 μg kg-1 (average 214 μg kg-1), ZON from 157.144 to 471.055 μg kg-1 (average 299.934 μg kg-1), and FB1 from 2.715 to 16.488 μg kg-1 (average 6.286 μg kg-1).
AB  - Učestalost gljiva i koncentracija mikotoksina deoksinivalenola (DON), zearalenona (ZON) i fumonizina (FB1) proučavana je u zrnu ozime pšenice prikupljene neposredno nakon žetve u 2010. godini. U ispitivanim uzorcima najveću zastupljenost imaju vrste iz roda Fusarium (84,7%), a znatno manje vrste iz roda Alternaria (12,35%), a posebno iz rodova Acremoniella (2,05%), Acremonium (0,65%) i Penicillium (0,25%). Od vrsta roda Fusarium najzastupljenija je vrsta F. graminearum (99,05%), dok su sporadične vrste F. sporotrichioides (0,4%), F. subglutinans (0,4%), F. poae (0,05%), F. proliferatum (0,05%) i F. verticillioides (0,05%). Prisustvo mikotoksina DON, ZON i FB1 ustanovljeno je u svim ispitivanim uzorcima zrna pšenice. DON je bio detektovan u koncentracijama od 123 do 393 μg kg-1 (prosek 214 μg kg-1), ZON od 157,144 do 471,055 μg kg-1 (prosek 299,934 μg kg-1) i FB1 od 2,715 do 16,488 μg kg-1 (prosek 6,286 μg kg-1).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Fusarium species and their mycotoxins in wheat grain
T1  - Fusarium vrste i njihovi mikotoksini u zrnu pšenice
IS  - 120
SP  - 41
EP  - 48
DO  - 10.2298/ZMSPN1120041K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Stepanić, Ana",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/378",
abstract = "Incidence of fungi and concentration of mycotoxin deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the grain of the winter wheat collected subsequently after harvesting in 2010. In the analyzed samples the highest incidence was determined for the species of Fusarium (84.7%) genus, significantly lower incidence was determined for the species of genus Alternaria (12.35%), and especially for species of genera Acremoniella (2.05%), Acremonium (0.65%) and Penicillium (0.25%). F. graminearum (99.05%) was the most present species of Fusarium genus, whereas the following species F. sporotrichioides (0.4%), F. subglutinans (0.4%), F. poae (0.05%), F. proliferatum (0.05%) and F. verticillioides (0.05%) were only sporadic. The presence of DON, ZON and FB1 mycotoxins was established in all studied wheat samples. DON was detected in concentrations from 123 to 393 μg kg-1 (average 214 μg kg-1), ZON from 157.144 to 471.055 μg kg-1 (average 299.934 μg kg-1), and FB1 from 2.715 to 16.488 μg kg-1 (average 6.286 μg kg-1)., Učestalost gljiva i koncentracija mikotoksina deoksinivalenola (DON), zearalenona (ZON) i fumonizina (FB1) proučavana je u zrnu ozime pšenice prikupljene neposredno nakon žetve u 2010. godini. U ispitivanim uzorcima najveću zastupljenost imaju vrste iz roda Fusarium (84,7%), a znatno manje vrste iz roda Alternaria (12,35%), a posebno iz rodova Acremoniella (2,05%), Acremonium (0,65%) i Penicillium (0,25%). Od vrsta roda Fusarium najzastupljenija je vrsta F. graminearum (99,05%), dok su sporadične vrste F. sporotrichioides (0,4%), F. subglutinans (0,4%), F. poae (0,05%), F. proliferatum (0,05%) i F. verticillioides (0,05%). Prisustvo mikotoksina DON, ZON i FB1 ustanovljeno je u svim ispitivanim uzorcima zrna pšenice. DON je bio detektovan u koncentracijama od 123 do 393 μg kg-1 (prosek 214 μg kg-1), ZON od 157,144 do 471,055 μg kg-1 (prosek 299,934 μg kg-1) i FB1 od 2,715 do 16,488 μg kg-1 (prosek 6,286 μg kg-1).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Fusarium species and their mycotoxins in wheat grain, Fusarium vrste i njihovi mikotoksini u zrnu pšenice",
number = "120",
pages = "41-48",
doi = "10.2298/ZMSPN1120041K"
}
Krnjaja, V., Lević, J., Stanković, S.,& Stepanić, A. (2011). Fusarium vrste i njihovi mikotoksini u zrnu pšenice.
Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(120), 41-48.
https://doi.org/10.2298/ZMSPN1120041K
Krnjaja V, Lević J, Stanković S, Stepanić A. Fusarium vrste i njihovi mikotoksini u zrnu pšenice. Zbornik Matice srpske za prirodne nauke. 2011;(120):41-48
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Stepanić Ana, "Fusarium vrste i njihovi mikotoksini u zrnu pšenice" Zbornik Matice srpske za prirodne nauke, no. 120 (2011):41-48,
https://doi.org/10.2298/ZMSPN1120041K .
4