Vasić, Tanja

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orcid::0000-0003-2312-0251
  • Vasić, Tanja (5)
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Author's Bibliography

Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains

Krnjaja, Vesna; Mandić, Violeta; Lukić, Miloš; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Vasić, Tanja
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/735
AB  - Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains
VL  - 116
SP  - 126
EP  - 131
DO  - 10.1016/j.cropro.2018.10.021
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lukić, Miloš and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Vasić, Tanja",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/735",
abstract = "Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains",
volume = "116",
pages = "126-131",
doi = "10.1016/j.cropro.2018.10.021"
}
Krnjaja, V., Mandić, V., Lukić, M., Bijelić, Z., Stanković, S., Obradović, A.,& Vasić, T. (2019). Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains.
Crop Protection
Elsevier Sci Ltd, Oxford., 116, 126-131.
https://doi.org/10.1016/j.cropro.2018.10.021
Krnjaja V, Mandić V, Lukić M, Bijelić Z, Stanković S, Obradović A, Vasić T. Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains. Crop Protection. 2019;116:126-131
Krnjaja Vesna, Mandić Violeta, Lukić Miloš, Bijelić Zorica, Stanković Slavica, Obradović Ana, Vasić Tanja, "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains" Crop Protection, 116 (2019):126-131,
https://doi.org/10.1016/j.cropro.2018.10.021 .
7
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Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover

Vasić, Tanja; Terzić, Dragan; Milenković, Jasmina; Marković, Jordan; Krnjaja, Vesna; Jevremović, Darko; Stanković, Slavica

(Društvo genetičara Srbije, Beograd, 2016)

TY  - JOUR
AU  - Vasić, Tanja
AU  - Terzić, Dragan
AU  - Milenković, Jasmina
AU  - Marković, Jordan
AU  - Krnjaja, Vesna
AU  - Jevremović, Darko
AU  - Stanković, Slavica
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/650
AB  - A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover
VL  - 48
IS  - 1
SP  - 187
EP  - 198
DO  - 10.2298/GENSR1601187V
ER  - 
@article{
author = "Vasić, Tanja and Terzić, Dragan and Milenković, Jasmina and Marković, Jordan and Krnjaja, Vesna and Jevremović, Darko and Stanković, Slavica",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/650",
abstract = "A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover",
volume = "48",
number = "1",
pages = "187-198",
doi = "10.2298/GENSR1601187V"
}
Vasić, T., Terzić, D., Milenković, J., Marković, J., Krnjaja, V., Jevremović, D.,& Stanković, S. (2016). Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover.
Genetika
Društvo genetičara Srbije, Beograd., 48(1), 187-198.
https://doi.org/10.2298/GENSR1601187V
Vasić T, Terzić D, Milenković J, Marković J, Krnjaja V, Jevremović D, Stanković S. Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. Genetika. 2016;48(1):187-198
Vasić Tanja, Terzić Dragan, Milenković Jasmina, Marković Jordan, Krnjaja Vesna, Jevremović Darko, Stanković Slavica, "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover" Genetika, 48, no. 1 (2016):187-198,
https://doi.org/10.2298/GENSR1601187V .

Fungal and mycotoxin contamination of maize hybrids in different maturity groups

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Bijelić, Zorica; Vasić, Tanja; Jauković, Marko

(Institut za stočarstvo, Beograd, 2016)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Vasić, Tanja
AU  - Jauković, Marko
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/627
AB  - In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods.
AB  - U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal and mycotoxin contamination of maize hybrids in different maturity groups
T1  - Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja
VL  - 32
IS  - 1
SP  - 71
EP  - 81
DO  - 10.2298/BAH1601071K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Vasić, Tanja and Jauković, Marko",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/627",
abstract = "In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods., U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal and mycotoxin contamination of maize hybrids in different maturity groups, Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja",
volume = "32",
number = "1",
pages = "71-81",
doi = "10.2298/BAH1601071K"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Bijelić, Z., Vasić, T.,& Jauković, M. (2016). Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(1), 71-81.
https://doi.org/10.2298/BAH1601071K
Krnjaja V, Stanković S, Obradović A, Mandić V, Bijelić Z, Vasić T, Jauković M. Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja. Biotechnology in Animal Husbandry. 2016;32(1):71-81
Krnjaja Vesna, Stanković Slavica, Obradović Ana, Mandić Violeta, Bijelić Zorica, Vasić Tanja, Jauković Marko, "Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja" Biotechnology in Animal Husbandry, 32, no. 1 (2016):71-81,
https://doi.org/10.2298/BAH1601071K .
4

Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat

Krnjaja, Vesna; Mandić, Violeta; Lević, Jelena; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/602
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
VL  - 67
SP  - 251
EP  - 256
DO  - 10.1016/j.cropro.2014.11.001
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/602",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
volume = "67",
pages = "251-256",
doi = "10.1016/j.cropro.2014.11.001"
}
Krnjaja, V., Mandić, V., Lević, J., Stanković, S., Obradović, A., Petrović, T.,& Vasić, T. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat.
Crop Protection
Elsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001
Krnjaja V, Mandić V, Lević J, Stanković S, Obradović A, Petrović T, Vasić T. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. Crop Protection. 2015;67:251-256
Krnjaja Vesna, Mandić Violeta, Lević Jelena, Stanković Slavica, Obradović Ana, Petrović Tanja, Vasić Tanja, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" Crop Protection, 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 .
15
8
13

The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Vasić, Tanja

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Vasić, Tanja
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/509
AB  - Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail.
AB  - Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi
T1  - Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva
VL  - 28
IS  - 3
SP  - 157
EP  - 165
DO  - 10.2298/PIF1303157K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Vasić, Tanja",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/509",
abstract = "Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail., Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi, Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva",
volume = "28",
number = "3",
pages = "157-165",
doi = "10.2298/PIF1303157K"
}
Krnjaja, V., Lević, J., Stanković, S.,& Vasić, T. (2013). Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva.
Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(3), 157-165.
https://doi.org/10.2298/PIF1303157K
Krnjaja V, Lević J, Stanković S, Vasić T. Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva. Pesticidi i fitomedicina. 2013;28(3):157-165
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Vasić Tanja, "Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva" Pesticidi i fitomedicina, 28, no. 3 (2013):157-165,
https://doi.org/10.2298/PIF1303157K .
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