Lukić, Miloš

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  • Lukić, Miloš (7)
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Mycobiota and aflatoxin B1 in poultry feeds

Krnjaja, Vesna; Petrović, Tanja; Stanković, Slavica; Lukić, Miloš; Škrbić, Zdenka; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Škrbić, Zdenka
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/742
AB  - In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.
AB  - U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Mycobiota and aflatoxin B1 in poultry feeds
T1  - Mikobiota i aflatoksin B1 u hrani za živinu
VL  - 35
IS  - 1
SP  - 61
EP  - 69
DO  - 10.2298/BAH1901061K
ER  - 
@article{
author = "Krnjaja, Vesna and Petrović, Tanja and Stanković, Slavica and Lukić, Miloš and Škrbić, Zdenka and Mandić, Violeta and Bijelić, Zorica",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/742",
abstract = "In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain., U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Mycobiota and aflatoxin B1 in poultry feeds, Mikobiota i aflatoksin B1 u hrani za živinu",
volume = "35",
number = "1",
pages = "61-69",
doi = "10.2298/BAH1901061K"
}
Krnjaja, V., Petrović, T., Stanković, S., Lukić, M., Škrbić, Z., Mandić, V.,& Bijelić, Z. (2019). Mikobiota i aflatoksin B1 u hrani za živinu.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 35(1), 61-69.
https://doi.org/10.2298/BAH1901061K
Krnjaja V, Petrović T, Stanković S, Lukić M, Škrbić Z, Mandić V, Bijelić Z. Mikobiota i aflatoksin B1 u hrani za živinu. Biotechnology in Animal Husbandry. 2019;35(1):61-69
Krnjaja Vesna, Petrović Tanja, Stanković Slavica, Lukić Miloš, Škrbić Zdenka, Mandić Violeta, Bijelić Zorica, "Mikobiota i aflatoksin B1 u hrani za živinu" Biotechnology in Animal Husbandry, 35, no. 1 (2019):61-69,
https://doi.org/10.2298/BAH1901061K .

Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains

Krnjaja, Vesna; Mandić, Violeta; Lukić, Miloš; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Vasić, Tanja
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/735
AB  - Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains
VL  - 116
SP  - 126
EP  - 131
DO  - 10.1016/j.cropro.2018.10.021
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lukić, Miloš and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Vasić, Tanja",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/735",
abstract = "Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains",
volume = "116",
pages = "126-131",
doi = "10.1016/j.cropro.2018.10.021"
}
Krnjaja, V., Mandić, V., Lukić, M., Bijelić, Z., Stanković, S., Obradović, A.,& Vasić, T. (2019). Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains.
Crop Protection
Elsevier Sci Ltd, Oxford., 116, 126-131.
https://doi.org/10.1016/j.cropro.2018.10.021
Krnjaja V, Mandić V, Lukić M, Bijelić Z, Stanković S, Obradović A, Vasić T. Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains. Crop Protection. 2019;116:126-131
Krnjaja Vesna, Mandić Violeta, Lukić Miloš, Bijelić Zorica, Stanković Slavica, Obradović Ana, Vasić Tanja, "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains" Crop Protection, 116 (2019):126-131,
https://doi.org/10.1016/j.cropro.2018.10.021 .
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Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia

Krnjaja, Vesna; Stanković, Slavica; Lukić, Miloš; Mićić, Nenad; Petrović, Tanja; Bijelić, Zorica; Mandić, Violeta

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Mićić, Nenad
AU  - Petrović, Tanja
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/720
AB  - This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.
AB  - Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia
T1  - Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji
VL  - 34
IS  - 2
SP  - 239
EP  - 249
DO  - 10.2298/bah1802239K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Lukić, Miloš and Mićić, Nenad and Petrović, Tanja and Bijelić, Zorica and Mandić, Violeta",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/720",
abstract = "This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain., Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia, Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji",
volume = "34",
number = "2",
pages = "239-249",
doi = "10.2298/bah1802239K"
}
Krnjaja, V., Stanković, S., Lukić, M., Mićić, N., Petrović, T., Bijelić, Z.,& Mandić, V. (2018). Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(2), 239-249.
https://doi.org/10.2298/bah1802239K
Krnjaja V, Stanković S, Lukić M, Mićić N, Petrović T, Bijelić Z, Mandić V. Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji. Biotechnology in Animal Husbandry. 2018;34(2):239-249
Krnjaja Vesna, Stanković Slavica, Lukić Miloš, Mićić Nenad, Petrović Tanja, Bijelić Zorica, Mandić Violeta, "Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji" Biotechnology in Animal Husbandry, 34, no. 2 (2018):239-249,
https://doi.org/10.2298/bah1802239K .
1

Fungal contamination of maize grain samples with a special focus on toxigenic genera

Krnjaja, Vesna; Stanojković, Aleksandar; Stanković, Slavica; Lukić, Miloš; Bijelić, Zorica; Mandić, Violeta; Mićić, Nenad

(Institut za stočarstvo, Beograd, 2017)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanojković, Aleksandar
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Mićić, Nenad
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/664
AB  - In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi.
AB  - Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal contamination of maize grain samples with a special focus on toxigenic genera
T1  - Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove
VL  - 33
IS  - 2
SP  - 233
EP  - 241
DO  - 10.2298/BAH1702233K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanojković, Aleksandar and Stanković, Slavica and Lukić, Miloš and Bijelić, Zorica and Mandić, Violeta and Mićić, Nenad",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/664",
abstract = "In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi., Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal contamination of maize grain samples with a special focus on toxigenic genera, Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove",
volume = "33",
number = "2",
pages = "233-241",
doi = "10.2298/BAH1702233K"
}
Krnjaja, V., Stanojković, A., Stanković, S., Lukić, M., Bijelić, Z., Mandić, V.,& Mićić, N. (2017). Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 33(2), 233-241.
https://doi.org/10.2298/BAH1702233K
Krnjaja V, Stanojković A, Stanković S, Lukić M, Bijelić Z, Mandić V, Mićić N. Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove. Biotechnology in Animal Husbandry. 2017;33(2):233-241
Krnjaja Vesna, Stanojković Aleksandar, Stanković Slavica, Lukić Miloš, Bijelić Zorica, Mandić Violeta, Mićić Nenad, "Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove" Biotechnology in Animal Husbandry, 33, no. 2 (2017):233-241,
https://doi.org/10.2298/BAH1702233K .
4

Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest

Bijelić, Zorica; Krnjaja, Vesna; Muslic-Ruzić, Dragana; Mandić, Violeta; Škrbić, Zdenka; Lukić, Miloš; Stanković, Slavica

(Ars Docendi, Bucharest, 2017)

TY  - JOUR
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Muslic-Ruzić, Dragana
AU  - Mandić, Violeta
AU  - Škrbić, Zdenka
AU  - Lukić, Miloš
AU  - Stanković, Slavica
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/688
AB  - The aim of this study was to determine the presence of fungi and concentrations of mycotoxins, zearalenone, fumonisin and deoxynivalenol, in lucerne and grass-legume silages, under the influence of different doses of N fertilization and harvesting at different stages of phenological development of the plants. Studies included pure lucerne crop and mixtures of lucerne with cocksfoot, tall fescue and sainfoin, sown in different ratios, fertilized with 0, 70, 140 or 210 kgN ha(-1) and harvested in the butonization phase or at 50% flowering of lucerne plants. Results showed that the total fungi count in the silage depended on the three investigated factors. The highest total fungi count was determined in the mixture silages of lucerne, cocksfoot and tall fescue, which was well treated with different nitrogen quantities, harvested and prepared in later stages of plant development. The most commonly-occurring fungi were Fusarium species (85.5% of fungi were this genus). Of the studied mycotoxins, only DON depended on fertilization, as it reduced the concentration of DON from 0.15 to 0.07 mg kg(-1). Preventing appearance of fungi and their mycotoxins in forage and silage should begin in the field as well as during the process of preparation of silage.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest
VL  - 22
IS  - 5
SP  - 12907
EP  - 12914
ER  - 
@article{
author = "Bijelić, Zorica and Krnjaja, Vesna and Muslic-Ruzić, Dragana and Mandić, Violeta and Škrbić, Zdenka and Lukić, Miloš and Stanković, Slavica",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/688",
abstract = "The aim of this study was to determine the presence of fungi and concentrations of mycotoxins, zearalenone, fumonisin and deoxynivalenol, in lucerne and grass-legume silages, under the influence of different doses of N fertilization and harvesting at different stages of phenological development of the plants. Studies included pure lucerne crop and mixtures of lucerne with cocksfoot, tall fescue and sainfoin, sown in different ratios, fertilized with 0, 70, 140 or 210 kgN ha(-1) and harvested in the butonization phase or at 50% flowering of lucerne plants. Results showed that the total fungi count in the silage depended on the three investigated factors. The highest total fungi count was determined in the mixture silages of lucerne, cocksfoot and tall fescue, which was well treated with different nitrogen quantities, harvested and prepared in later stages of plant development. The most commonly-occurring fungi were Fusarium species (85.5% of fungi were this genus). Of the studied mycotoxins, only DON depended on fertilization, as it reduced the concentration of DON from 0.15 to 0.07 mg kg(-1). Preventing appearance of fungi and their mycotoxins in forage and silage should begin in the field as well as during the process of preparation of silage.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest",
volume = "22",
number = "5",
pages = "12907-12914"
}
Bijelić, Z., Krnjaja, V., Muslic-Ruzić, D., Mandić, V., Škrbić, Z., Lukić, M.,& Stanković, S. (2017). Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest.
Romanian Biotechnological Letters
Ars Docendi, Bucharest., 22(5), 12907-12914.
Bijelić Z, Krnjaja V, Muslic-Ruzić D, Mandić V, Škrbić Z, Lukić M, Stanković S. Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest. Romanian Biotechnological Letters. 2017;22(5):12907-12914
Bijelić Zorica, Krnjaja Vesna, Muslic-Ruzić Dragana, Mandić Violeta, Škrbić Zdenka, Lukić Miloš, Stanković Slavica, "Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest" Romanian Biotechnological Letters, 22, no. 5 (2017):12907-12914
1

Molds and mycotoxins in freshly harvested maize

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, Tanja; Lukić, Miloš

(Matica srpska, Novi Sad, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, Tanja
AU  - Lukić, Miloš
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/516
AB  - Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02).
AB  - Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Molds and mycotoxins in freshly harvested maize
T1  - Plesni i mikotoksini u zrnu kukuruza posle berbe
IS  - 124
SP  - 111
EP  - 119
DO  - 10.2298/ZMSPN1324111K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, Tanja and Lukić, Miloš",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/516",
abstract = "Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02)., Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Molds and mycotoxins in freshly harvested maize, Plesni i mikotoksini u zrnu kukuruza posle berbe",
number = "124",
pages = "111-119",
doi = "10.2298/ZMSPN1324111K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T.,& Lukić, M. (2013). Plesni i mikotoksini u zrnu kukuruza posle berbe.
Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(124), 111-119.
https://doi.org/10.2298/ZMSPN1324111K
Krnjaja V, Lević J, Stanković S, Petrović T, Lukić M. Plesni i mikotoksini u zrnu kukuruza posle berbe. Zbornik Matice srpske za prirodne nauke. 2013;(124):111-119
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović Tanja, Lukić Miloš, "Plesni i mikotoksini u zrnu kukuruza posle berbe" Zbornik Matice srpske za prirodne nauke, no. 124 (2013):111-119,
https://doi.org/10.2298/ZMSPN1324111K .
2

Nitrogen effects on maize susceptibility to Fusarium ear rot (Fusarium verticillioides)

Krnjaja, Vesna; Nešić, Zorica D.; Radović, Čedomir; Lukić, Miloš; Stanković, Slavica

(Cereal Res Inst, Szeged, 2008)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Nešić, Zorica D.
AU  - Radović, Čedomir
AU  - Lukić, Miloš
AU  - Stanković, Slavica
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/248
AB  - Ear rot caused by Fusarium species is serious problem for maize growers. Fusarium car rot, European corn borer attack and length of car in maize were assessed after inoculation with Fusarium verticillioides in condition of fertilization with different sources of nitrogen. No effect of fertilization on increase of the intensity of incidence of all investigated parameters was established, whereas considerably increased intensity of Fusarium car rot in plants inoculated with F. verticillioides in all investigated fertilization treatments was established.
PB  - Cereal Res Inst, Szeged
T2  - Cereal Research Communications
T1  - Nitrogen effects on maize susceptibility to Fusarium ear rot (Fusarium verticillioides)
VL  - 36
SP  - 579
EP  - 580
DO  - 10.1556/CRC.36.2008.Suppl.B.48
ER  - 
@article{
author = "Krnjaja, Vesna and Nešić, Zorica D. and Radović, Čedomir and Lukić, Miloš and Stanković, Slavica",
year = "2008",
url = "http://rik.mrizp.rs/handle/123456789/248",
abstract = "Ear rot caused by Fusarium species is serious problem for maize growers. Fusarium car rot, European corn borer attack and length of car in maize were assessed after inoculation with Fusarium verticillioides in condition of fertilization with different sources of nitrogen. No effect of fertilization on increase of the intensity of incidence of all investigated parameters was established, whereas considerably increased intensity of Fusarium car rot in plants inoculated with F. verticillioides in all investigated fertilization treatments was established.",
publisher = "Cereal Res Inst, Szeged",
journal = "Cereal Research Communications",
title = "Nitrogen effects on maize susceptibility to Fusarium ear rot (Fusarium verticillioides)",
volume = "36",
pages = "579-580",
doi = "10.1556/CRC.36.2008.Suppl.B.48"
}
Krnjaja, V., Nešić, Z. D., Radović, Č., Lukić, M.,& Stanković, S. (2008). Nitrogen effects on maize susceptibility to Fusarium ear rot (Fusarium verticillioides).
Cereal Research Communications
Cereal Res Inst, Szeged., 36, 579-580.
https://doi.org/10.1556/CRC.36.2008.Suppl.B.48
Krnjaja V, Nešić ZD, Radović Č, Lukić M, Stanković S. Nitrogen effects on maize susceptibility to Fusarium ear rot (Fusarium verticillioides). Cereal Research Communications. 2008;36:579-580
Krnjaja Vesna, Nešić Zorica D., Radović Čedomir, Lukić Miloš, Stanković Slavica, "Nitrogen effects on maize susceptibility to Fusarium ear rot (Fusarium verticillioides)" Cereal Research Communications, 36 (2008):579-580,
https://doi.org/10.1556/CRC.36.2008.Suppl.B.48 .
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