TY  - JOUR
AU  - Kovačević, Tamara
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Vukojević, Jelena
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/513
AB  - The status of fertility and distribution of mating populations in the G. fujikuroi species complex, isolating from maize, sorghum and wheat cultivated under various agroecological conditions of Serbia, have been studied. A total of 79 field isolates of Fusarium spp. in the section Liseola, which had been reciprocally crossed to standard testers (MAT-1 and MAT-2) from each of the four mating populations of the G. fujikuroi species complex, were selected for these studies. Twenty of 79 isolates belonged to the mating population A (G. moniliformis), 22 to the population D (G. intermedia), 17 to the population E (G. subglutinans) and 20 to the population F (G. thapsina). A mating type MAT-1 was dominant in the populations A (14 MATA-1 :6 MATA-2), D (13 MATA-1 :9 MATA-2) and E (10 MATA-1 :7 MATA-2), while MAT-2 prevailed in the population F (6 MATA-1 : 14 MATA-2). The obtained results indicate that the possibility of sexual reproduction of Fusarium spp., belonging to the A, D, E and F mating populations, is not so frequent phenomenon in Serbia as in other regions world-wide. Consequently, these species will be asexually reproduced under field conditions, particularly species belonging to the F population. These are the first results on the characterisation of three (A, E and F) out of four populations of the G. fujikuroi species complex present in Serbia.
AB  - U radu su proučavani fertilnost i distribucija polnih populacija i tipova u okviru kompleksne vrste G. fujikuroi, poreklom iz različitih biljnih vrsta (kukuruza, gajenog sirka i pšenice), koje su gajene u različitim godinama i agroekološkim uslovima Srbije. Za proučavanja su odabrana 79 poljskih izolata Fusarium spp. iz sekcije Liseola, koji su recipročno ukršteni sa standardnim testerima (MAT-1 i MAT-2) od četiri polne populacije kompleksne vrste G. fujikuroi. U ukrštanjima je fertilno potomstvo dalo 77 od 79 izolata. Od 77 izolata 20 je pripadalo polnoj populaciji A (G. moniliformis), 22 populaciji D (G. intermedia), 15 populaciji E (G. subglutinans) i 20 populaciji F (G. thapsina). Polni tip MAT-1 je dominirao u populacijama A (14 MATA-1 :6 MATA-2), D (13 MATA-1 :9 MATA-2) iE(10 MATA-1 :7 MATA-2), dok je MAT-2 dominirao u populaciji F (6 MATA-1 : 14 MATA-2). Proučavani izolati su bili fertilni kao očevi a sterilni kao majke, izuzev po jedan izolat u populacijama A, D i E koji su bili hermafroditi. Na osnovu polnog tipa (Ne(mt)) efektivni broj za A i F populacije bio je 84.00%, za D je 96.69% i E je 96.89%. Na osnovu učestalosti hermafrodita efektivan broj (Ne(f)) za populaciju A bio je 18.15%, populaciju D 16.60%, populaciju E 20.84% i populaciju F 0.00% od stvarnog broja. Dobijeni rezultati ukazuju da je mogućnost seksualne reprodukcije Fusarium spp., koje pripadaju utvrđenim polnim populacija, nije učestala pojava u Srbiji, kao što je za njih utvrđeno u drugim regionima sveta. Kao posledica toga, ove vrste će se aseksualno reprodukovati u poljskim uslovima, posebno vrsta koja pripada F populaciji. Ovo su prvi rezultati o karakterizaciji tri (A, E i F) od četiri populacije kompleksne vrste G. fujikuroi, koje su prisutne u Srbiji.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia
T1  - Polne populacije kompleksne vrste Gibberella fujikuroi (Sawada) S. Ito poreklom iz kukuruza, gajenog sirka i pšenice u Srbiji
VL  - 45
IS  - 3
SP  - 749
EP  - 760
DO  - 10.2298/GENSR1303749K
ER  - 

@article{
author = "Kovačević, Tamara and Lević, Jelena and Stanković, Slavica and Vukojević, Jelena",
year = "2013",
abstract = "The status of fertility and distribution of mating populations in the G. fujikuroi species complex, isolating from maize, sorghum and wheat cultivated under various agroecological conditions of Serbia, have been studied. A total of 79 field isolates of Fusarium spp. in the section Liseola, which had been reciprocally crossed to standard testers (MAT-1 and MAT-2) from each of the four mating populations of the G. fujikuroi species complex, were selected for these studies. Twenty of 79 isolates belonged to the mating population A (G. moniliformis), 22 to the population D (G. intermedia), 17 to the population E (G. subglutinans) and 20 to the population F (G. thapsina). A mating type MAT-1 was dominant in the populations A (14 MATA-1 :6 MATA-2), D (13 MATA-1 :9 MATA-2) and E (10 MATA-1 :7 MATA-2), while MAT-2 prevailed in the population F (6 MATA-1 : 14 MATA-2). The obtained results indicate that the possibility of sexual reproduction of Fusarium spp., belonging to the A, D, E and F mating populations, is not so frequent phenomenon in Serbia as in other regions world-wide. Consequently, these species will be asexually reproduced under field conditions, particularly species belonging to the F population. These are the first results on the characterisation of three (A, E and F) out of four populations of the G. fujikuroi species complex present in Serbia., U radu su proučavani fertilnost i distribucija polnih populacija i tipova u okviru kompleksne vrste G. fujikuroi, poreklom iz različitih biljnih vrsta (kukuruza, gajenog sirka i pšenice), koje su gajene u različitim godinama i agroekološkim uslovima Srbije. Za proučavanja su odabrana 79 poljskih izolata Fusarium spp. iz sekcije Liseola, koji su recipročno ukršteni sa standardnim testerima (MAT-1 i MAT-2) od četiri polne populacije kompleksne vrste G. fujikuroi. U ukrštanjima je fertilno potomstvo dalo 77 od 79 izolata. Od 77 izolata 20 je pripadalo polnoj populaciji A (G. moniliformis), 22 populaciji D (G. intermedia), 15 populaciji E (G. subglutinans) i 20 populaciji F (G. thapsina). Polni tip MAT-1 je dominirao u populacijama A (14 MATA-1 :6 MATA-2), D (13 MATA-1 :9 MATA-2) iE(10 MATA-1 :7 MATA-2), dok je MAT-2 dominirao u populaciji F (6 MATA-1 : 14 MATA-2). Proučavani izolati su bili fertilni kao očevi a sterilni kao majke, izuzev po jedan izolat u populacijama A, D i E koji su bili hermafroditi. Na osnovu polnog tipa (Ne(mt)) efektivni broj za A i F populacije bio je 84.00%, za D je 96.69% i E je 96.89%. Na osnovu učestalosti hermafrodita efektivan broj (Ne(f)) za populaciju A bio je 18.15%, populaciju D 16.60%, populaciju E 20.84% i populaciju F 0.00% od stvarnog broja. Dobijeni rezultati ukazuju da je mogućnost seksualne reprodukcije Fusarium spp., koje pripadaju utvrđenim polnim populacija, nije učestala pojava u Srbiji, kao što je za njih utvrđeno u drugim regionima sveta. Kao posledica toga, ove vrste će se aseksualno reprodukovati u poljskim uslovima, posebno vrsta koja pripada F populaciji. Ovo su prvi rezultati o karakterizaciji tri (A, E i F) od četiri populacije kompleksne vrste G. fujikuroi, koje su prisutne u Srbiji.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia, Polne populacije kompleksne vrste Gibberella fujikuroi (Sawada) S. Ito poreklom iz kukuruza, gajenog sirka i pšenice u Srbiji",
volume = "45",
number = "3",
pages = "749-760",
doi = "10.2298/GENSR1303749K"
}

Kovačević, T., Lević, J., Stanković, S.,& Vukojević, J.. (2013). Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 45(3), 749-760.
https://doi.org/10.2298/GENSR1303749K

Kovačević T, Lević J, Stanković S, Vukojević J. Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia. in Genetika. 2013;45(3):749-760.
doi:10.2298/GENSR1303749K .

Kovačević, Tamara, Lević, Jelena, Stanković, Slavica, Vukojević, Jelena, "Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia" in Genetika, 45, no. 3 (2013):749-760,
https://doi.org/10.2298/GENSR1303749K . .

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Ivanović, Dragica
AU  - Kovačević, Tamara
AU  - Tančić, Sonja
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/242
AB  - The objective of this study was to identify crop production factor, associated with FHB development in Serbian wheat. FHB index and weight losses, varying from 16% to 74% and from 1.8% to 38.3%, respectively, indicated that enviromental conditions were the most important factor affecting disease development and yield losses. F. graminearum dominated on kernels (tip to 55.5%) and whole rachis (34.7%), while F poae was predominant on apical part od rachis (35.2%).
PB  - Cereal Res Inst, Szeged
T2  - Cereal Research Communications
T1  - Fusarium head blight and grain yield losses of wheat in Serbia
VL  - 36
SP  - 513
EP  - 514
DO  - 10.1556/CRC.36.2008.Suppl.B.43
ER  - 

@article{
author = "Lević, Jelena and Stanković, Slavica and Ivanović, Dragica and Kovačević, Tamara and Tančić, Sonja and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra",
year = "2008",
abstract = "The objective of this study was to identify crop production factor, associated with FHB development in Serbian wheat. FHB index and weight losses, varying from 16% to 74% and from 1.8% to 38.3%, respectively, indicated that enviromental conditions were the most important factor affecting disease development and yield losses. F. graminearum dominated on kernels (tip to 55.5%) and whole rachis (34.7%), while F poae was predominant on apical part od rachis (35.2%).",
publisher = "Cereal Res Inst, Szeged",
journal = "Cereal Research Communications",
title = "Fusarium head blight and grain yield losses of wheat in Serbia",
volume = "36",
pages = "513-514",
doi = "10.1556/CRC.36.2008.Suppl.B.43"
}

Lević, J., Stanković, S., Ivanović, D., Kovačević, T., Tančić, S., Krnjaja, V.,& Bočarov-Stančić, A.. (2008). Fusarium head blight and grain yield losses of wheat in Serbia. in Cereal Research Communications
Cereal Res Inst, Szeged., 36, 513-514.
https://doi.org/10.1556/CRC.36.2008.Suppl.B.43

Lević J, Stanković S, Ivanović D, Kovačević T, Tančić S, Krnjaja V, Bočarov-Stančić A. Fusarium head blight and grain yield losses of wheat in Serbia. in Cereal Research Communications. 2008;36:513-514.
doi:10.1556/CRC.36.2008.Suppl.B.43 .

Lević, Jelena, Stanković, Slavica, Ivanović, Dragica, Kovačević, Tamara, Tančić, Sonja, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, "Fusarium head blight and grain yield losses of wheat in Serbia" in Cereal Research Communications, 36 (2008):513-514,
https://doi.org/10.1556/CRC.36.2008.Suppl.B.43 . .

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Kovačević, Tamara
AU  - Tančić, Sonja
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/243
AB  - Effects of nine isolates of F. langsethiae, originating from Serbia (2005-2006), on vigour of wheat seedlings were the objective of this study. Symptoms of seedlings root rot, reduced of seed germination and retardation of coleoptiles and roots growth were observed. Growth of seedlings roots treated with the filtrate of isolates was reduced in comparison to control. Isolates of F. langsethiae were separated into three VCGs.
PB  - Cereal Res Inst, Szeged
T2  - Cereal Research Communications
T1  - Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings
VL  - 36
SP  - 515
EP  - 516
DO  - 10.1556/CRC.36.2008.Suppl.B.43
ER  - 

@article{
author = "Lević, Jelena and Stanković, Slavica and Kovačević, Tamara and Tančić, Sonja and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra",
year = "2008",
abstract = "Effects of nine isolates of F. langsethiae, originating from Serbia (2005-2006), on vigour of wheat seedlings were the objective of this study. Symptoms of seedlings root rot, reduced of seed germination and retardation of coleoptiles and roots growth were observed. Growth of seedlings roots treated with the filtrate of isolates was reduced in comparison to control. Isolates of F. langsethiae were separated into three VCGs.",
publisher = "Cereal Res Inst, Szeged",
journal = "Cereal Research Communications",
title = "Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings",
volume = "36",
pages = "515-516",
doi = "10.1556/CRC.36.2008.Suppl.B.43"
}

Lević, J., Stanković, S., Kovačević, T., Tančić, S., Krnjaja, V.,& Bočarov-Stančić, A.. (2008). Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings. in Cereal Research Communications
Cereal Res Inst, Szeged., 36, 515-516.
https://doi.org/10.1556/CRC.36.2008.Suppl.B.43

Lević J, Stanković S, Kovačević T, Tančić S, Krnjaja V, Bočarov-Stančić A. Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings. in Cereal Research Communications. 2008;36:515-516.
doi:10.1556/CRC.36.2008.Suppl.B.43 .

Lević, Jelena, Stanković, Slavica, Kovačević, Tamara, Tančić, Sonja, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, "Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings" in Cereal Research Communications, 36 (2008):515-516,
https://doi.org/10.1556/CRC.36.2008.Suppl.B.43 . .

TY  - JOUR
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
AU  - Tančić, Sonja
AU  - Kovačević, Tamara
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/195
AB  - A total of 88 and 40 wheat samples collected immediately prior to harvest in 2005 and 2006, respectively, under different agroecological conditions, were studied in respect to the occurrence of Fusarium spp. and the production of fusariotoxins. The greatest number of samples was infected with species of the genera Fusarium (81.8 and 65.0%), and Alternaria (36.3 and 17.5%) with the intensity ranging from 9.4 to 84.0% in 2005 and from 23.4 to 80.6% in 2006. Out of 13 identified species belonging to the genus Fusarium, F. graminearum had the highest frequency (35.2 and 12.5%) and the intensity up to 67.2%, and 21.9%, in 2005 and 2006, respectively, followed by F. poae but only in 2005 (20.4%), and F. proliferatum in 2006 (19.7%). The natural occurrence of mycotoxins in positive samples varied from 37 to 331 ppb for zearalenone and from 31 to 125 ppb for diacetoxyscirpenol (DAS) and T-2 toxin. The concentration of mycotoxins amounted, on average, to 133.4, 61.0 and 45.7 ppb for zearelenone, DAS and T-2 toxin, respectively.
AB  - Osamdeset osam i 40 uzoraka pšenice, prikupljenih neposredno pred žetvu u 2005. i 2006. godini u različitim agroekološkim uslovima u Srbiji proučavani su radi praćenja pojave Fusarium spp. i stvaranja fuzariotoksina. Najveći broj uzoraka bio je zaražen vrstama roda Fusarium (81,8 i 65,0%), a zatim sa Alternaria spp. (36,3 i 17,5%) sa intenzitetom 9,4-84,0% u 2005. i 23,4-80,6% u 2006. godini. Od ukupno 13 identifikovanih vrsta iz roda Fusarium najučestalija je bila F. graminearum (35,2 i 12,5%) sa intenzitetom do 67,2% u 2005. i do 21,9% u 2006. godini, a zatim F. poae, ali samo u 2005. godini (20,4%), i F. proliferatum u 2006. godini (19.7%). Prirodna pojava mikotoksina u pozitivnim uzorcima je varirala od 37 do 331 ppb za zearalenon i od 31 do 125 ppb za diacetoksiscirpenol (DAS) i T-2 toksin. U proseku, koncentracija mikotoksina je bila 133,4 ppb za zearelonon, 61,0 ppb za DAS i 45,7 ppb za T-2 toksin.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia
T1  - Učestalost toksigenih vrsta Fusarium i fuzariotoksina u zrnu pšenice u Srbiji
IS  - 113
SP  - 93
EP  - 102
DO  - 10.2298/ZMSPN0713093S
ER  - 

@article{
author = "Stanković, Slavica and Lević, Jelena and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra and Tančić, Sonja and Kovačević, Tamara",
year = "2007",
abstract = "A total of 88 and 40 wheat samples collected immediately prior to harvest in 2005 and 2006, respectively, under different agroecological conditions, were studied in respect to the occurrence of Fusarium spp. and the production of fusariotoxins. The greatest number of samples was infected with species of the genera Fusarium (81.8 and 65.0%), and Alternaria (36.3 and 17.5%) with the intensity ranging from 9.4 to 84.0% in 2005 and from 23.4 to 80.6% in 2006. Out of 13 identified species belonging to the genus Fusarium, F. graminearum had the highest frequency (35.2 and 12.5%) and the intensity up to 67.2%, and 21.9%, in 2005 and 2006, respectively, followed by F. poae but only in 2005 (20.4%), and F. proliferatum in 2006 (19.7%). The natural occurrence of mycotoxins in positive samples varied from 37 to 331 ppb for zearalenone and from 31 to 125 ppb for diacetoxyscirpenol (DAS) and T-2 toxin. The concentration of mycotoxins amounted, on average, to 133.4, 61.0 and 45.7 ppb for zearelenone, DAS and T-2 toxin, respectively., Osamdeset osam i 40 uzoraka pšenice, prikupljenih neposredno pred žetvu u 2005. i 2006. godini u različitim agroekološkim uslovima u Srbiji proučavani su radi praćenja pojave Fusarium spp. i stvaranja fuzariotoksina. Najveći broj uzoraka bio je zaražen vrstama roda Fusarium (81,8 i 65,0%), a zatim sa Alternaria spp. (36,3 i 17,5%) sa intenzitetom 9,4-84,0% u 2005. i 23,4-80,6% u 2006. godini. Od ukupno 13 identifikovanih vrsta iz roda Fusarium najučestalija je bila F. graminearum (35,2 i 12,5%) sa intenzitetom do 67,2% u 2005. i do 21,9% u 2006. godini, a zatim F. poae, ali samo u 2005. godini (20,4%), i F. proliferatum u 2006. godini (19.7%). Prirodna pojava mikotoksina u pozitivnim uzorcima je varirala od 37 do 331 ppb za zearalenon i od 31 do 125 ppb za diacetoksiscirpenol (DAS) i T-2 toksin. U proseku, koncentracija mikotoksina je bila 133,4 ppb za zearelonon, 61,0 ppb za DAS i 45,7 ppb za T-2 toksin.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia, Učestalost toksigenih vrsta Fusarium i fuzariotoksina u zrnu pšenice u Srbiji",
number = "113",
pages = "93-102",
doi = "10.2298/ZMSPN0713093S"
}

Stanković, S., Lević, J., Krnjaja, V., Bočarov-Stančić, A., Tančić, S.,& Kovačević, T.. (2007). Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 93-102.
https://doi.org/10.2298/ZMSPN0713093S

Stanković S, Lević J, Krnjaja V, Bočarov-Stančić A, Tančić S, Kovačević T. Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia. in Zbornik Matice srpske za prirodne nauke. 2007;(113):93-102.
doi:10.2298/ZMSPN0713093S .

Stanković, Slavica, Lević, Jelena, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, Tančić, Sonja, Kovačević, Tamara, "Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):93-102,
https://doi.org/10.2298/ZMSPN0713093S . .

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Kovačević, Tamara
AU  - Tančić, Sonja
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/197
AB  - Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively.
AB  - U ovom radu je u in vitro uslovima proučena sposobnost izolata F. poae za biosintezu jedne grupe fuzariotoksina - trihotecena tipa A (T-2 toksin i diacetoksiscirpenol - DAS), kao i zearalenona (ZEA). Toksikološki profil ove vrste je kod nas nedovoljno ispitan s obzirom na njenu zastupljenost i toksigena svojstva prema literaturnim podacima. Proučavanjima je bilo obuhvaćeno 11 izolata F. poae, poreklom sa pšenice iz 9 lokaliteta, uglavnom sa područja Vojvodine. Najveći broj uzoraka prikupljen je 2005. godine, kada su klimatski uslovi pogodovali intenzivnijoj pojavi fuzarioza klasa ove poljoprivredne kulture. Za određivanje toksikološkog potencijala F. poae kulture odabranih izolata su gajene u tečnim podlogama (GPK i SPK) tokom 3 dana na sobnoj temperaturi (21-26°C) i na rotacionoj tresilici (180 obrtaja min-1). Sirovi toksini su izolovani iz filtrata tečnih kultura ispitanih izolata pomoću etil acetata, dok je kvantifikacija mikotoksina izvršena metodom tankoslojne hromatografije. Tečna kultura izolata GZ-LES (F. graminearum) je korišćena kao kontrolna kultura za utvrđivanje potencijala za biosintezu zearalenona, a KF-38/1/R (F. sporotrichioides) za oba trihotecena tipa A (T-2 toksin i DAS). Dobijeni rezultati pokazuju da F. poae, za razliku od F. graminearum, ne poseduje potencijal za biosintezu zearalenona. Prisustvo DAS-a je utvrđeno samo kod jedne kulture F. poae (MRIZP-666) i kontrolnog izolata F. sporotrichioides (KF-38/1/R) koji su gajeni u tečnom GPK medijumu. T-2 toksin je detektovan kod izolata MRIZP-666 pri gajenju u obema podlogama, kao i izolata MRIZP-37 u GPK, odnosno MRIZP-860 u SPK medijumu. Kontrolna kultura KF-38/1/R (F. sporotrichioides) proizvodila je T-2 toksin u koncentraciji od 4000 μg L-1. Na osnovu iznetih podataka može se zaključiti da je potencijal F. poae za biosintezu trihotecena tipa A bio nizak u datim uslovima s obzirom da koncentracija DAS-a nije prelazila 80 μg L-1, odnosno T-2 toksina 240 μg L-1. Imajući u vidu prikazane rezultate, smatramo da je za dobijanje konačnog odgovora na pitanje o toksikološkom profilu izolata F. poae u Srbiji neophodno preduzeti dodatna ispitivanja, ne samo sa novim izolatima iz godina koje slede, nego i u drugim uslovima kultivisanja, u prvom redu na sterilnom prirodnom supstratu kao što su pšenica i kukuruz.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - The toxigenic potential of Fusarium poae originated from wheat
T1  - Toksigeni potencijal izolata Fusarium poae poreklom sa pšenice
IS  - 113
SP  - 113
EP  - 123
DO  - 10.2298/ZMSPN0713113B
ER  - 

@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Stanković, Slavica and Krnjaja, Vesna and Kovačević, Tamara and Tančić, Sonja",
year = "2007",
abstract = "Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively., U ovom radu je u in vitro uslovima proučena sposobnost izolata F. poae za biosintezu jedne grupe fuzariotoksina - trihotecena tipa A (T-2 toksin i diacetoksiscirpenol - DAS), kao i zearalenona (ZEA). Toksikološki profil ove vrste je kod nas nedovoljno ispitan s obzirom na njenu zastupljenost i toksigena svojstva prema literaturnim podacima. Proučavanjima je bilo obuhvaćeno 11 izolata F. poae, poreklom sa pšenice iz 9 lokaliteta, uglavnom sa područja Vojvodine. Najveći broj uzoraka prikupljen je 2005. godine, kada su klimatski uslovi pogodovali intenzivnijoj pojavi fuzarioza klasa ove poljoprivredne kulture. Za određivanje toksikološkog potencijala F. poae kulture odabranih izolata su gajene u tečnim podlogama (GPK i SPK) tokom 3 dana na sobnoj temperaturi (21-26°C) i na rotacionoj tresilici (180 obrtaja min-1). Sirovi toksini su izolovani iz filtrata tečnih kultura ispitanih izolata pomoću etil acetata, dok je kvantifikacija mikotoksina izvršena metodom tankoslojne hromatografije. Tečna kultura izolata GZ-LES (F. graminearum) je korišćena kao kontrolna kultura za utvrđivanje potencijala za biosintezu zearalenona, a KF-38/1/R (F. sporotrichioides) za oba trihotecena tipa A (T-2 toksin i DAS). Dobijeni rezultati pokazuju da F. poae, za razliku od F. graminearum, ne poseduje potencijal za biosintezu zearalenona. Prisustvo DAS-a je utvrđeno samo kod jedne kulture F. poae (MRIZP-666) i kontrolnog izolata F. sporotrichioides (KF-38/1/R) koji su gajeni u tečnom GPK medijumu. T-2 toksin je detektovan kod izolata MRIZP-666 pri gajenju u obema podlogama, kao i izolata MRIZP-37 u GPK, odnosno MRIZP-860 u SPK medijumu. Kontrolna kultura KF-38/1/R (F. sporotrichioides) proizvodila je T-2 toksin u koncentraciji od 4000 μg L-1. Na osnovu iznetih podataka može se zaključiti da je potencijal F. poae za biosintezu trihotecena tipa A bio nizak u datim uslovima s obzirom da koncentracija DAS-a nije prelazila 80 μg L-1, odnosno T-2 toksina 240 μg L-1. Imajući u vidu prikazane rezultate, smatramo da je za dobijanje konačnog odgovora na pitanje o toksikološkom profilu izolata F. poae u Srbiji neophodno preduzeti dodatna ispitivanja, ne samo sa novim izolatima iz godina koje slede, nego i u drugim uslovima kultivisanja, u prvom redu na sterilnom prirodnom supstratu kao što su pšenica i kukuruz.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "The toxigenic potential of Fusarium poae originated from wheat, Toksigeni potencijal izolata Fusarium poae poreklom sa pšenice",
number = "113",
pages = "113-123",
doi = "10.2298/ZMSPN0713113B"
}

Bočarov-Stančić, A., Lević, J., Stanković, S., Krnjaja, V., Kovačević, T.,& Tančić, S.. (2007). The toxigenic potential of Fusarium poae originated from wheat. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 113-123.
https://doi.org/10.2298/ZMSPN0713113B

Bočarov-Stančić A, Lević J, Stanković S, Krnjaja V, Kovačević T, Tančić S. The toxigenic potential of Fusarium poae originated from wheat. in Zbornik Matice srpske za prirodne nauke. 2007;(113):113-123.
doi:10.2298/ZMSPN0713113B .

Bočarov-Stančić, Aleksandra, Lević, Jelena, Stanković, Slavica, Krnjaja, Vesna, Kovačević, Tamara, Tančić, Sonja, "The toxigenic potential of Fusarium poae originated from wheat" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):113-123,
https://doi.org/10.2298/ZMSPN0713113B . .