Bočarov-Stančić, Aleksandra

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  • Bočarov-Stančić, Aleksandra (17)
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In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia

Bočarov-Stančić, Aleksandra; Lopičić, Zorica R.; Bodroža-Solarov, Marija I.; Stanković, Slavica; Janković, Snežana; Milojković, Jelena V.; Krulj, Jelena A.

(Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad, 2018)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lopičić, Zorica R.
AU  - Bodroža-Solarov, Marija I.
AU  - Stanković, Slavica
AU  - Janković, Snežana
AU  - Milojković, Jelena V.
AU  - Krulj, Jelena A.
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/718
AB  - Aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON) and T-2 toxin are the most extensively studied toxic fungal metabolites. Once mycotoxins enter the food/feed production chain keeping their toxic characteristics, it is very difficult to remove or eliminate them. One of promising methods to reduce mycotoxins in contaminated food/feedstuffs is the use of mycotoxin binders. This paper presents the results of in vitro investigations of mineral mycotoxin binders (bentonite - BEN, diatomite - DIA and zeolite - ZEO), and organic mycotoxin binders - agricultural waste materials (Myriophillium spicatum, peach and sour cherry pits). Chemical compositions of the adsorbents have showed that they do not consist of elements toxic to the animals. Inorganic adsorbents (BEN, DIA and ZEO) tested in vitro were better binders of AFB1 (94.97% - 96.90%), while the biosorbents were more efficient in adsorption of OTA (19.98% - 66.66%), ZON (33.33% - 75.00%) and T-2 toxin (16.67% - 50.00%). Inorganic adsorbents and organic waste materials expressed similar binding capacity for DON in vitro, with the exception of M. spicatum that did not at all adsorb this type B trichothecene. Our results indicate that feed contamination with different types of mycotoxins might be diminished by a product that combines different inorganic and organic adsorbents with diverse mycotoxin binding properties.
AB  - Aflatoksin B1 (AFB1), ohratoksin A (OTA), zearalenon (ZON), dezoksinivalenol (DON) i T-2 toksin su najviše izučavani toksični metaboliti gljiva. Kada mikotoksini uđu u proizvodni lanac za hranu/hranu za životinje, zadržavajući svoje toksične karakteristike, teško ih je ukloniti ili eliminisati. Jedna od obećavajućih metoda za smanjenje nivoa mikotoksina u kontaminiranoj hrani/hrani za životinje je korišćenje mikotoksinskih veziva. Ovaj rad predstavlja rezultate in vitro istraživanja mineralnih mikotoksinskih veziva (bentonit - BEN, diatomit - DIA i zeolit - ZEO) i organskih veziva mikotoksina - poljoprivrednog otpadnog materijala (Myriophillium spicatum, koštice breskve i višnje). Hemijski sastavi adsorbenata pokazali su da ne sadrže elemente toksične za životinje. Neorganski adsorbenti (BEN, DIA i ZEO) testirani in vitro bolje su vezivali AFB1 (94,97% - 96,90%), dok su biosorbenti bili efikasniji u adsorpciji OTA (19,98% - 66,66%), ZON-a (33,33% - 75,00% ) i T-2 toksina (16,67% - 50,00%). Neorganski adsorbenti i organski otpadni materijali su pokazali sličan kapacitet in vitro vezivanja DON-a, sa izuzetkom M. spicatum koji uopšte nije adsorbovao ovaj trihotecen tipa B. Naši rezultati koji su prikazani ovde pokazuju da zagađivanje hrane i hrane za životinje različitim vrstama mikotoksina može biti smanjeno dodavanjem preparata dobijenog kombinacijom različitih neorganskih i organskih adsorbenata koji poseduju različite karakteristike vezivanja mikotoksina.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia
T1  - In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije
VL  - 45
IS  - 7
SP  - 87
EP  - 96
DO  - 10.5937/FFR1802087B
UR  - conv_657
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lopičić, Zorica R. and Bodroža-Solarov, Marija I. and Stanković, Slavica and Janković, Snežana and Milojković, Jelena V. and Krulj, Jelena A.",
year = "2018",
abstract = "Aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON) and T-2 toxin are the most extensively studied toxic fungal metabolites. Once mycotoxins enter the food/feed production chain keeping their toxic characteristics, it is very difficult to remove or eliminate them. One of promising methods to reduce mycotoxins in contaminated food/feedstuffs is the use of mycotoxin binders. This paper presents the results of in vitro investigations of mineral mycotoxin binders (bentonite - BEN, diatomite - DIA and zeolite - ZEO), and organic mycotoxin binders - agricultural waste materials (Myriophillium spicatum, peach and sour cherry pits). Chemical compositions of the adsorbents have showed that they do not consist of elements toxic to the animals. Inorganic adsorbents (BEN, DIA and ZEO) tested in vitro were better binders of AFB1 (94.97% - 96.90%), while the biosorbents were more efficient in adsorption of OTA (19.98% - 66.66%), ZON (33.33% - 75.00%) and T-2 toxin (16.67% - 50.00%). Inorganic adsorbents and organic waste materials expressed similar binding capacity for DON in vitro, with the exception of M. spicatum that did not at all adsorb this type B trichothecene. Our results indicate that feed contamination with different types of mycotoxins might be diminished by a product that combines different inorganic and organic adsorbents with diverse mycotoxin binding properties., Aflatoksin B1 (AFB1), ohratoksin A (OTA), zearalenon (ZON), dezoksinivalenol (DON) i T-2 toksin su najviše izučavani toksični metaboliti gljiva. Kada mikotoksini uđu u proizvodni lanac za hranu/hranu za životinje, zadržavajući svoje toksične karakteristike, teško ih je ukloniti ili eliminisati. Jedna od obećavajućih metoda za smanjenje nivoa mikotoksina u kontaminiranoj hrani/hrani za životinje je korišćenje mikotoksinskih veziva. Ovaj rad predstavlja rezultate in vitro istraživanja mineralnih mikotoksinskih veziva (bentonit - BEN, diatomit - DIA i zeolit - ZEO) i organskih veziva mikotoksina - poljoprivrednog otpadnog materijala (Myriophillium spicatum, koštice breskve i višnje). Hemijski sastavi adsorbenata pokazali su da ne sadrže elemente toksične za životinje. Neorganski adsorbenti (BEN, DIA i ZEO) testirani in vitro bolje su vezivali AFB1 (94,97% - 96,90%), dok su biosorbenti bili efikasniji u adsorpciji OTA (19,98% - 66,66%), ZON-a (33,33% - 75,00% ) i T-2 toksina (16,67% - 50,00%). Neorganski adsorbenti i organski otpadni materijali su pokazali sličan kapacitet in vitro vezivanja DON-a, sa izuzetkom M. spicatum koji uopšte nije adsorbovao ovaj trihotecen tipa B. Naši rezultati koji su prikazani ovde pokazuju da zagađivanje hrane i hrane za životinje različitim vrstama mikotoksina može biti smanjeno dodavanjem preparata dobijenog kombinacijom različitih neorganskih i organskih adsorbenata koji poseduju različite karakteristike vezivanja mikotoksina.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia, In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije",
volume = "45",
number = "7",
pages = "87-96",
doi = "10.5937/FFR1802087B",
url = "conv_657"
}
Bočarov-Stančić, A., Lopičić, Z. R., Bodroža-Solarov, M. I., Stanković, S., Janković, S., Milojković, J. V.,& Krulj, J. A.. (2018). In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 45(7), 87-96.
https://doi.org/10.5937/FFR1802087B
conv_657
Bočarov-Stančić A, Lopičić ZR, Bodroža-Solarov MI, Stanković S, Janković S, Milojković JV, Krulj JA. In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia. in Food and Feed Research. 2018;45(7):87-96.
doi:10.5937/FFR1802087B
conv_657 .
Bočarov-Stančić, Aleksandra, Lopičić, Zorica R., Bodroža-Solarov, Marija I., Stanković, Slavica, Janković, Snežana, Milojković, Jelena V., Krulj, Jelena A., "In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia" in Food and Feed Research, 45, no. 7 (2018):87-96,
https://doi.org/10.5937/FFR1802087B .,
conv_657 .
1

Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels

Krstović, Saša Z.; Jakšić, Sandra M.; Bočarov-Stančić, Aleksandra; Stanković, Slavica; Janković, Snežana; Jajić, Igor M.

(Matica srpska, Novi Sad, 2017)

TY  - JOUR
AU  - Krstović, Saša Z.
AU  - Jakšić, Sandra M.
AU  - Bočarov-Stančić, Aleksandra
AU  - Stanković, Slavica
AU  - Janković, Snežana
AU  - Jajić, Igor M.
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/662
AB  - The production of fumonisins by potentially toxigenic Fusarium verticillioides isolates originating from Serbian maize and wheat kernels was tested in vitro. A total of six F. verticillioides isolates were incubated on yeast extract sucrose medium (YESA) for 4 weeks at 25 °C in the dark. Their toxin production potential was tested by applying a modified HPLC method for determination of fumonisins in cereals, since the TLC method gave no results. Analyses were performed on a HPLC-FLD system after sample extraction from YESA and extract clean-up on a SPE column. Although the isolates were tested for fumonisin B1, B2 and B3, only fumonisin B1 was detected. The results showed that all tested isolates had toxigenic potential for fumonisin B1 production. The average fumonisin B1 production of the isolates ranged from 7 to 289 µg/kg, thus indicating a highly variable toxigenic potential among the isolates. Isolate 1282 expressed the highest toxigenic potential for fumonisin B1 production (289 µg/kg), while isolate 2533/A showed a questionable potential for fumonisin production (7 µg/kg).
AB  - Proizvodnja fumonizina kod potencijalno toksikogenih Fusarium verticillioides izolata sa zrna kukuruza i pšenice poreklom iz Srbije testiran je in vitro. Ukupno šest izolata F. verticillioides inkubirano je na agarizovanoj podlozi sa ekstraktom kvasca i saharozom (YESA) tokom četiri nedelje, u mraku na 25 °C. Kapaciteti odabranih izolata za proizvodnju toksina detektovani su primenom modifikovane HPLC metode za određivanje fumonizina u zrnu žitarica, jer brza trijažna TLC metoda nije dala pozitivne rezultate. Analiza je izvedena na HPLC-FLD sistemu nakon ekstrakcije uzorka iz YESA kultura izolata i prečišćavanja ekstrakta na SPE koloni. Sve analize su urađene u tri ponavljanja. Iako su izolati F. verticillioides testirani na prisustvo fumonizina B1, B2 i B3, samo je fumonizin B1 bio detektovan. Rezultati su pokazali da svi testirani izolati imaju potencijal u sintezi fumonizina B1. Prosečna vrednost proizvedenog fumonizina B1 kod izolata F. verticillioides kretala se od 7 do 289 µg/kg, što ukazuje na izuzetno varijabilan toksigeni potencijal istih izolata. Izolat označen sa 1282 pokazao je najveći potencijal za biosintezu fumonizina B1 (289 µg/kg), dok je izolat 2533/A ispoljio diskutabilan potencijal za proizvodnju istog fumonizina (7 µg/kg).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels
T1  - Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije
IS  - 133
SP  - 71
EP  - 78
DO  - 10.2298/ZMSPN1733071K
UR  - conv_65
ER  - 
@article{
author = "Krstović, Saša Z. and Jakšić, Sandra M. and Bočarov-Stančić, Aleksandra and Stanković, Slavica and Janković, Snežana and Jajić, Igor M.",
year = "2017",
abstract = "The production of fumonisins by potentially toxigenic Fusarium verticillioides isolates originating from Serbian maize and wheat kernels was tested in vitro. A total of six F. verticillioides isolates were incubated on yeast extract sucrose medium (YESA) for 4 weeks at 25 °C in the dark. Their toxin production potential was tested by applying a modified HPLC method for determination of fumonisins in cereals, since the TLC method gave no results. Analyses were performed on a HPLC-FLD system after sample extraction from YESA and extract clean-up on a SPE column. Although the isolates were tested for fumonisin B1, B2 and B3, only fumonisin B1 was detected. The results showed that all tested isolates had toxigenic potential for fumonisin B1 production. The average fumonisin B1 production of the isolates ranged from 7 to 289 µg/kg, thus indicating a highly variable toxigenic potential among the isolates. Isolate 1282 expressed the highest toxigenic potential for fumonisin B1 production (289 µg/kg), while isolate 2533/A showed a questionable potential for fumonisin production (7 µg/kg)., Proizvodnja fumonizina kod potencijalno toksikogenih Fusarium verticillioides izolata sa zrna kukuruza i pšenice poreklom iz Srbije testiran je in vitro. Ukupno šest izolata F. verticillioides inkubirano je na agarizovanoj podlozi sa ekstraktom kvasca i saharozom (YESA) tokom četiri nedelje, u mraku na 25 °C. Kapaciteti odabranih izolata za proizvodnju toksina detektovani su primenom modifikovane HPLC metode za određivanje fumonizina u zrnu žitarica, jer brza trijažna TLC metoda nije dala pozitivne rezultate. Analiza je izvedena na HPLC-FLD sistemu nakon ekstrakcije uzorka iz YESA kultura izolata i prečišćavanja ekstrakta na SPE koloni. Sve analize su urađene u tri ponavljanja. Iako su izolati F. verticillioides testirani na prisustvo fumonizina B1, B2 i B3, samo je fumonizin B1 bio detektovan. Rezultati su pokazali da svi testirani izolati imaju potencijal u sintezi fumonizina B1. Prosečna vrednost proizvedenog fumonizina B1 kod izolata F. verticillioides kretala se od 7 do 289 µg/kg, što ukazuje na izuzetno varijabilan toksigeni potencijal istih izolata. Izolat označen sa 1282 pokazao je najveći potencijal za biosintezu fumonizina B1 (289 µg/kg), dok je izolat 2533/A ispoljio diskutabilan potencijal za proizvodnju istog fumonizina (7 µg/kg).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels, Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije",
number = "133",
pages = "71-78",
doi = "10.2298/ZMSPN1733071K",
url = "conv_65"
}
Krstović, S. Z., Jakšić, S. M., Bočarov-Stančić, A., Stanković, S., Janković, S.,& Jajić, I. M.. (2017). Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(133), 71-78.
https://doi.org/10.2298/ZMSPN1733071K
conv_65
Krstović SZ, Jakšić SM, Bočarov-Stančić A, Stanković S, Janković S, Jajić IM. Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels. in Zbornik Matice srpske za prirodne nauke. 2017;(133):71-78.
doi:10.2298/ZMSPN1733071K
conv_65 .
Krstović, Saša Z., Jakšić, Sandra M., Bočarov-Stančić, Aleksandra, Stanković, Slavica, Janković, Snežana, Jajić, Igor M., "Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels" in Zbornik Matice srpske za prirodne nauke, no. 133 (2017):71-78,
https://doi.org/10.2298/ZMSPN1733071K .,
conv_65 .

An outbreak of Aspergillus species in response to environmental conditions in Serbia

Lević, Jelena; Gošić-Dondo, Snežana; Ivanović, Dragica; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra; Stepanić, Ana

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Gošić-Dondo, Snežana
AU  - Ivanović, Dragica
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/510
AB  - The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low.
AB  - U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - An outbreak of Aspergillus species in response to environmental conditions in Serbia
T1  - Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji
VL  - 28
IS  - 3
SP  - 167
EP  - 179
DO  - 10.2298/PIF1303167L
UR  - conv_607
ER  - 
@article{
author = "Lević, Jelena and Gošić-Dondo, Snežana and Ivanović, Dragica and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra and Stepanić, Ana",
year = "2013",
abstract = "The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low., U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "An outbreak of Aspergillus species in response to environmental conditions in Serbia, Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji",
volume = "28",
number = "3",
pages = "167-179",
doi = "10.2298/PIF1303167L",
url = "conv_607"
}
Lević, J., Gošić-Dondo, S., Ivanović, D., Stanković, S., Krnjaja, V., Bočarov-Stančić, A.,& Stepanić, A.. (2013). An outbreak of Aspergillus species in response to environmental conditions in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(3), 167-179.
https://doi.org/10.2298/PIF1303167L
conv_607
Lević J, Gošić-Dondo S, Ivanović D, Stanković S, Krnjaja V, Bočarov-Stančić A, Stepanić A. An outbreak of Aspergillus species in response to environmental conditions in Serbia. in Pesticidi i fitomedicina. 2013;28(3):167-179.
doi:10.2298/PIF1303167L
conv_607 .
Lević, Jelena, Gošić-Dondo, Snežana, Ivanović, Dragica, Stanković, Slavica, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, Stepanić, Ana, "An outbreak of Aspergillus species in response to environmental conditions in Serbia" in Pesticidi i fitomedicina, 28, no. 3 (2013):167-179,
https://doi.org/10.2298/PIF1303167L .,
conv_607 .
32

Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia

Lević, Jelena; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra; Ivanović, Dragica

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
AU  - Ivanović, Dragica
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/454
AB  - A total of 41 species of fungi were isolated from seed samples of barley, maize, soybean, and sunflower collected at different locations in Serbia. The majority of detected species occurred on barley (35 of 41 species or 87.8%) comparing to soybean (17 of 41 species or 41.5%), sunflower (16 of 41 species or 39.0%) and maize (15 of 41 species or 36.9%). Species belonging to genera Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium and Rhizopus were present on seeds of all four plant species. Alternaria species were dominant on soybean, barley and sunflower seeds (85.7%, 84.7% and 76.9%). F. verticillioides and Penicillium spp. were mainly isolated from maize seeds (100 and 92.3% respectively), while other species were isolated up to 38.5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae and F. sporotrichioides were the most common Fusarium species isolated from barley (51.1-93.3%), while on the soybean seeds F. oxysporum (71.4%), F. semitectum (57.1%) and F. sporotrichioides (57.1%) were prevalent. Frequency of Fusarium species on sunflower seeds varied from 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) to 15.4% (F. verticillioides). Statistically significant negative correlation (r = -0.678*) was determined for the incidence of F. graminearum and Alternaria spp., as well as, Fusarium spp. and Alternaria spp. (r = -0.614*), on barley seeds. The obtained results revealed that seedborne pathogens were present in most seed samples of important cereals and industrial crops grown under different agroecological conditions in Serbia. Some of the identified fungi are potential producers of mycotoxins, thus their presence is important in terms of reduced food safety for humans and animals. Therefore, an early and accurate diagnosis and pathogen surveillance will provide time for the development and the application of disease strategies.
AB  - Ukupno je izolovana 41 vrsta gljiva iz uzoraka zrna ječma, kukuruza, soje i suncokreta, koji su prikupljeni u različitim lokalitetima u Srbiji. Najveći broj gljiva je utvrđen na zrnu ječma (35 vrsta ili 87,8%) u odnosu na zrno soje (17 vrsta ili 41,5%), suncokreta (16 vrsta ili 39,0%) i kukuruza (15 vrsta ili 36,9%). Vrste rodova Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium i Rhizopus su utvrđene na zrnu sve četiri biljne vrste. Vrste roda Alternaria su dominirale na zrnu ječma (99,5%), suncokreta (52,9%) i soje (50,0%). F. verticillioides (do 92,0%) i Penicillium spp. (do 57,0%) su najčešće izolovane vrste sa zrna kukuruza, dok su ostale vrste izolovane do 38,5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae i F. sporotrichioides su najčešće Fusarium vrste izolovane sa zrna ječma (51,1-93,3%), dok su na zrnu soje dominirale F. oxysporum (71,4%), F. semitectum (57,1%) i F. sporotrichioides (57,1%). Učestalost Fusarium vrsta na zrnu suncokreta je varirala od 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) do 15,4% (F. verticillioides). Na zrnu ječma utvđena je statistički značajna negativna korelacija između intenziteta napada F. graminearum i Alternaria spp. (r = -0,678*), kao i između Fusarium spp. i Alternaria spp. (r = -0,614*). Dobijeni rezultati pokazuju da su patogene gljive učestale i u visokom intenzitetu pojave prisutne na zrnu važnih žita i industrijskog bilja gajenih u različitim agroekološkim uslovima u Srbiji. Neke od identifikovanih gljiva su potencijalni proizvođači mikotoksina i njihovo prisustvo je važno zbog smanjene bezbednosti hrane za ljude i životinje. Stoga, rana i pouzdana identifikacija patogenih gljiva i procena njihovog značaja u proizvodnji kukuruza, strnih žita i industrijskih biljaka, omogućuje da se pravovremeno razvije i primeni strategija za njihovo suzbijanje i ublažavanje šteta.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia
T1  - Pojava i učestalost patogena semena žita i industrijskih biljaka u Srbiji
VL  - 27
IS  - 1
SP  - 33
EP  - 40
DO  - 10.2298/PIF1201033L
UR  - conv_603
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra and Ivanović, Dragica",
year = "2012",
abstract = "A total of 41 species of fungi were isolated from seed samples of barley, maize, soybean, and sunflower collected at different locations in Serbia. The majority of detected species occurred on barley (35 of 41 species or 87.8%) comparing to soybean (17 of 41 species or 41.5%), sunflower (16 of 41 species or 39.0%) and maize (15 of 41 species or 36.9%). Species belonging to genera Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium and Rhizopus were present on seeds of all four plant species. Alternaria species were dominant on soybean, barley and sunflower seeds (85.7%, 84.7% and 76.9%). F. verticillioides and Penicillium spp. were mainly isolated from maize seeds (100 and 92.3% respectively), while other species were isolated up to 38.5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae and F. sporotrichioides were the most common Fusarium species isolated from barley (51.1-93.3%), while on the soybean seeds F. oxysporum (71.4%), F. semitectum (57.1%) and F. sporotrichioides (57.1%) were prevalent. Frequency of Fusarium species on sunflower seeds varied from 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) to 15.4% (F. verticillioides). Statistically significant negative correlation (r = -0.678*) was determined for the incidence of F. graminearum and Alternaria spp., as well as, Fusarium spp. and Alternaria spp. (r = -0.614*), on barley seeds. The obtained results revealed that seedborne pathogens were present in most seed samples of important cereals and industrial crops grown under different agroecological conditions in Serbia. Some of the identified fungi are potential producers of mycotoxins, thus their presence is important in terms of reduced food safety for humans and animals. Therefore, an early and accurate diagnosis and pathogen surveillance will provide time for the development and the application of disease strategies., Ukupno je izolovana 41 vrsta gljiva iz uzoraka zrna ječma, kukuruza, soje i suncokreta, koji su prikupljeni u različitim lokalitetima u Srbiji. Najveći broj gljiva je utvrđen na zrnu ječma (35 vrsta ili 87,8%) u odnosu na zrno soje (17 vrsta ili 41,5%), suncokreta (16 vrsta ili 39,0%) i kukuruza (15 vrsta ili 36,9%). Vrste rodova Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium i Rhizopus su utvrđene na zrnu sve četiri biljne vrste. Vrste roda Alternaria su dominirale na zrnu ječma (99,5%), suncokreta (52,9%) i soje (50,0%). F. verticillioides (do 92,0%) i Penicillium spp. (do 57,0%) su najčešće izolovane vrste sa zrna kukuruza, dok su ostale vrste izolovane do 38,5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae i F. sporotrichioides su najčešće Fusarium vrste izolovane sa zrna ječma (51,1-93,3%), dok su na zrnu soje dominirale F. oxysporum (71,4%), F. semitectum (57,1%) i F. sporotrichioides (57,1%). Učestalost Fusarium vrsta na zrnu suncokreta je varirala od 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) do 15,4% (F. verticillioides). Na zrnu ječma utvđena je statistički značajna negativna korelacija između intenziteta napada F. graminearum i Alternaria spp. (r = -0,678*), kao i između Fusarium spp. i Alternaria spp. (r = -0,614*). Dobijeni rezultati pokazuju da su patogene gljive učestale i u visokom intenzitetu pojave prisutne na zrnu važnih žita i industrijskog bilja gajenih u različitim agroekološkim uslovima u Srbiji. Neke od identifikovanih gljiva su potencijalni proizvođači mikotoksina i njihovo prisustvo je važno zbog smanjene bezbednosti hrane za ljude i životinje. Stoga, rana i pouzdana identifikacija patogenih gljiva i procena njihovog značaja u proizvodnji kukuruza, strnih žita i industrijskih biljaka, omogućuje da se pravovremeno razvije i primeni strategija za njihovo suzbijanje i ublažavanje šteta.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia, Pojava i učestalost patogena semena žita i industrijskih biljaka u Srbiji",
volume = "27",
number = "1",
pages = "33-40",
doi = "10.2298/PIF1201033L",
url = "conv_603"
}
Lević, J., Stanković, S., Krnjaja, V., Bočarov-Stančić, A.,& Ivanović, D.. (2012). Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 27(1), 33-40.
https://doi.org/10.2298/PIF1201033L
conv_603
Lević J, Stanković S, Krnjaja V, Bočarov-Stančić A, Ivanović D. Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia. in Pesticidi i fitomedicina. 2012;27(1):33-40.
doi:10.2298/PIF1201033L
conv_603 .
Lević, Jelena, Stanković, Slavica, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, Ivanović, Dragica, "Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia" in Pesticidi i fitomedicina, 27, no. 1 (2012):33-40,
https://doi.org/10.2298/PIF1201033L .,
conv_603 .
14

Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root

Lević, Jelena; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra

(Elsevier Sci Ltd, Oxford, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/410
AB  - The present study describes the developed filter layer method for the consistent determination and identification of the natural occurrence of Pyrenochaeta terrestris in maize roots. The field experiment was set up to establish the applicability, accuracy and efficiency of this method for the pathogen isolation from roots of maize grown under different agroecological conditions. According to this method, the 10- to 15-mm long tissue sections, mainly cut at the margin of lesions developed in roots, were placed on two layers of moisten filter paper in Petri dishes and incubated for 6-7 days under laboratory conditions, 25 +/- 2 degrees C and daylight. The unique reddish-purple (beet-red) pigmentation was used as an indicator of the fungal incidence in infected roots, which facilitated a succeeding confirmation of the pathogen on PDA at 25 degrees C in the dark and on CLA in the alternating 12 h light (fluorescent and near-ultraviolet light)/12 h dark conditions. Obtained results showed that the filter layer method was very effective for the P. terrestris detection and that it could be applied for a rapid assessment of response of maize genotypes, environmental and seasonal effects on the fungal growth on roots and the fungal distribution. Advantages of this method for the detection and quantification of P. terrestris in relation to the methods described in the literature include: a) low investments, because many root samples can be tested immediately at the same time; b) results can be obtained rapidly (6-7 days after sampling); and c) minimal interference by other fungi or bacteria.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root
VL  - 38
SP  - 66
EP  - 71
DO  - 10.1016/j.cropro.2012.03.015
UR  - conv_816
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra",
year = "2012",
abstract = "The present study describes the developed filter layer method for the consistent determination and identification of the natural occurrence of Pyrenochaeta terrestris in maize roots. The field experiment was set up to establish the applicability, accuracy and efficiency of this method for the pathogen isolation from roots of maize grown under different agroecological conditions. According to this method, the 10- to 15-mm long tissue sections, mainly cut at the margin of lesions developed in roots, were placed on two layers of moisten filter paper in Petri dishes and incubated for 6-7 days under laboratory conditions, 25 +/- 2 degrees C and daylight. The unique reddish-purple (beet-red) pigmentation was used as an indicator of the fungal incidence in infected roots, which facilitated a succeeding confirmation of the pathogen on PDA at 25 degrees C in the dark and on CLA in the alternating 12 h light (fluorescent and near-ultraviolet light)/12 h dark conditions. Obtained results showed that the filter layer method was very effective for the P. terrestris detection and that it could be applied for a rapid assessment of response of maize genotypes, environmental and seasonal effects on the fungal growth on roots and the fungal distribution. Advantages of this method for the detection and quantification of P. terrestris in relation to the methods described in the literature include: a) low investments, because many root samples can be tested immediately at the same time; b) results can be obtained rapidly (6-7 days after sampling); and c) minimal interference by other fungi or bacteria.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root",
volume = "38",
pages = "66-71",
doi = "10.1016/j.cropro.2012.03.015",
url = "conv_816"
}
Lević, J., Stanković, S., Krnjaja, V.,& Bočarov-Stančić, A.. (2012). Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root. in Crop Protection
Elsevier Sci Ltd, Oxford., 38, 66-71.
https://doi.org/10.1016/j.cropro.2012.03.015
conv_816
Lević J, Stanković S, Krnjaja V, Bočarov-Stančić A. Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root. in Crop Protection. 2012;38:66-71.
doi:10.1016/j.cropro.2012.03.015
conv_816 .
Lević, Jelena, Stanković, Slavica, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, "Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root" in Crop Protection, 38 (2012):66-71,
https://doi.org/10.1016/j.cropro.2012.03.015 .,
conv_816 .
1
1

Relationships of mycobiota on rachides and kernels of wheat

Lević, Jelena; Stanković, Slavica; Ivanović, Dragica; Krnjaja, Vesna; Tančić, Sonja; Bočarov-Stančić, Aleksandra

(Springer, Dordrecht, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Ivanović, Dragica
AU  - Krnjaja, Vesna
AU  - Tančić, Sonja
AU  - Bočarov-Stančić, Aleksandra
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/418
AB  - The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14 wheat cultivars grown under 19 different agroecological conditions in Serbia, were studied. Out of the 23 identified fungal genera, a significantly higher number of species was isolated and identified from rachides (22) than from kernels of wheat (9). Fusarium and Alternaria species were the most frequent (up to 100 %) species on both, rachides and kernels, but the incidence of these fungi were higher on rachides than on kernels. The most frequent of the 14 Fusarium species were F. graminearum (96.8 % on both, rachides and kernels) and F. poae (93.8 % on rachides and 51.6 % on kernels). The frequency of F. verticillioides was significantly higher on rachides (64.5 %) than on kernels (19.4 %). A positive correlation (r = 0.5356 **) was established between the frequency of F. graminearum on rachides and on kernels. Furthermore, the frequency of Alternaria spp. was also statistically higher on rachides than on kernels, but the correlation was not statistically significant (r = 0.1729). The incidence of F. graminearum was negatively correlated with the incidence of Alternaria species in both, rachides (r = -0.3783 *) and kernels (r = -0.4863 **). These are the first data on the frequency and incidence of fungi on wheat rachides in Serbia, and they support the few data presented in the world literature. Results of this research could be useful for better understanding of pathways in a fungal infection and the improvement of wheat breeding for resistance, as well as, a proper application of fungicides in the wheat head protection.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Relationships of mycobiota on rachides and kernels of wheat
VL  - 134
IS  - 2
SP  - 249
EP  - 256
DO  - 10.1007/s10658-012-9982-8
UR  - conv_824
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Ivanović, Dragica and Krnjaja, Vesna and Tančić, Sonja and Bočarov-Stančić, Aleksandra",
year = "2012",
abstract = "The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14 wheat cultivars grown under 19 different agroecological conditions in Serbia, were studied. Out of the 23 identified fungal genera, a significantly higher number of species was isolated and identified from rachides (22) than from kernels of wheat (9). Fusarium and Alternaria species were the most frequent (up to 100 %) species on both, rachides and kernels, but the incidence of these fungi were higher on rachides than on kernels. The most frequent of the 14 Fusarium species were F. graminearum (96.8 % on both, rachides and kernels) and F. poae (93.8 % on rachides and 51.6 % on kernels). The frequency of F. verticillioides was significantly higher on rachides (64.5 %) than on kernels (19.4 %). A positive correlation (r = 0.5356 **) was established between the frequency of F. graminearum on rachides and on kernels. Furthermore, the frequency of Alternaria spp. was also statistically higher on rachides than on kernels, but the correlation was not statistically significant (r = 0.1729). The incidence of F. graminearum was negatively correlated with the incidence of Alternaria species in both, rachides (r = -0.3783 *) and kernels (r = -0.4863 **). These are the first data on the frequency and incidence of fungi on wheat rachides in Serbia, and they support the few data presented in the world literature. Results of this research could be useful for better understanding of pathways in a fungal infection and the improvement of wheat breeding for resistance, as well as, a proper application of fungicides in the wheat head protection.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Relationships of mycobiota on rachides and kernels of wheat",
volume = "134",
number = "2",
pages = "249-256",
doi = "10.1007/s10658-012-9982-8",
url = "conv_824"
}
Lević, J., Stanković, S., Ivanović, D., Krnjaja, V., Tančić, S.,& Bočarov-Stančić, A.. (2012). Relationships of mycobiota on rachides and kernels of wheat. in European Journal of Plant Pathology
Springer, Dordrecht., 134(2), 249-256.
https://doi.org/10.1007/s10658-012-9982-8
conv_824
Lević J, Stanković S, Ivanović D, Krnjaja V, Tančić S, Bočarov-Stančić A. Relationships of mycobiota on rachides and kernels of wheat. in European Journal of Plant Pathology. 2012;134(2):249-256.
doi:10.1007/s10658-012-9982-8
conv_824 .
Lević, Jelena, Stanković, Slavica, Ivanović, Dragica, Krnjaja, Vesna, Tančić, Sonja, Bočarov-Stančić, Aleksandra, "Relationships of mycobiota on rachides and kernels of wheat" in European Journal of Plant Pathology, 134, no. 2 (2012):249-256,
https://doi.org/10.1007/s10658-012-9982-8 .,
conv_824 .
6
8
9

The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A

Bočarov-Stančić, Aleksandra; Lević, Jelena; Salma, Nataša M.; Stanković, Slavica; Pantić, Vladimir R.; Dolić, Bisera J.

(Matica srpska, Novi Sad, 2011)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Salma, Nataša M.
AU  - Stanković, Slavica
AU  - Pantić, Vladimir R.
AU  - Dolić, Bisera J.
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/386
AB  - Nine isolates of fungi of genera Aspergillus, Fusarium, Paecilomyces and Penicillium were cultured on the modified Vogel's medium with the addition of crude ochratoxin A (OTA) extract. This crude OTA extract was derived from a natural solid substrate on which Aspergillus ochraceus strain CBS 108.08 was cultivated. OTA was isolated, partially purified, dried by evaporating and dissolved in ethanol (1 mg ml-1), and added to the test medium up to the final concentration of 10 μg ml-1. The presence of OTA residues was determined after 7 and 14 day cultivation of fungi in the test medium at 27±1°C. The Paecilomyces lilacinus isolate (Inf. 2/A), which completely degraded OTA (150 μg) after only seven days, was selected for further studies. Wet sterile rice grains (50 g + 25 ml distilled water) were inoculated with individual isolates of fungi A. ochraceus (strain CBS 108.08) and P. lilacinus (isolate Inf. 2/A), and with their combination. In the case of P. lilacinus monoculture, 0.9 mg of crude OTA was also added into cultivation substrate. Each test was done in three replications. After the four week cultivation of individual and combined fungi at 27±1°C, inoculated rice grains were dried to the constant weight and pulverized. OTA was determined in these samples by the application of standard TLC method for fodder analysis. OTA in the amount of 61.310 μg kg-1 dry matter (DM) was determined only in the samples inoculated with a producer of ochratoxin A (A. ochraceus, strain CBS 108.08). On the other hand, a much smaller amount of OTA (80 μg kg-1 DM) was detected in samples inoculated with combined cultures of A. ochraceus and P. lilacinus isolates. Gained results indicate that P. lilacinus degraded, on average, 99.8% of OTA. After four week cultivation, the same fungal isolate in the samples of wet sterile rice kernels with the addition of 0.9 mg of crude OTA, completely degraded added crude OTA ( lt 8 μg kg-1).
AB  - Devet izolata gljiva iz rodova Aspergillus, Fusarium, Paecilomyces i Penicillium gajeno je na modifikovanoj Vogelovoj podlozi sa dodatkom sirovog ekstrakta ohratoksina A (OTA). Sirovi ekstrakt OTA je dobijen iz čvrstog prirodnog supstrata na kojem je gajen soj Aspergillus ochraceus CBS 108.08. Izolovan i delimično prečišćen OTA, uparen do suvog ostatka i rastvoren u etanolu (1 mg ml-1), dodat je u test podlogu do finalne koncentracije 10 μg ml-1. Nakon sedam i 14 dana gajenja kultura gljiva u test podlozi na 27 ± 1°C de terminisano je prisustvo rezidua OTA primenom modifikovane metode Filtenborg-a i sar. (1983). Od devet testiranih izolata za dalja ispitivanja je odabran izolat Paecilomyces lilacinus (Inf. 2/A), koji je već posle sedam dana u potpunosti razgradio inicijalnu količinu OTA (150 μg). U drugom delu eksperimenta vlažno sterilno zrno pirinča (50 g + 25 ml destilovane vode) zasejano je sa pojedinačnim izolatima A. ochraceus (CBS 108.08) i P. lilacinus (Inf. 2-A), kao i kombinacijom oba izolata. U slučaju mono-kulture P. lilacinus u podlogu je dodat i sirovi OTA (0,9 mg). Svaki od testova je urađen u 3 ponavljanja. Nakon četiri nedelje gajenja monokultura i mešanih kul tura gljiva na 27±1°C, inokulisana zrna su osušena do konstantne težine i sa mlevena do finog praha. U ovim uzorcima izvršena je determinacija OTA primenom standardne metode tankoslojne hromatografije za analizu stočne hrane. U uzorcima koji su bili zasejani samo sa producentom OTA (A. ochraceus, soj CBS 108.08) detektovan je OTA u prosečnoj količini od 61.310 μg kg-1 suvog ostatka. U uzorcima koji su bili zasejani kombinovanim kulturama izolata A. ochraceus i P. lilacinus utvrđena je znatno manja prosečna količina OTA (80 μg kg-1). Ovi rezultati ukazuju da je izolat P. lilacinus razgradio prosečno 99,8% OTA prisutnog u podlozi za kultivaciju. U uzorcima vlažnog sterilnog zrna pirinča sa dodatkom 0,9 mg sirovog OTA isti gljivični izolat je posle četiri nedelje kultivacije kompletno biorazgradio dodat sirovi OTA ( lt 8 μg kg-1).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A
T1  - Uloga Paecilomyces lilacinus (Thom) Samson i drugih vrsta gljiva u biodegradaciji ohratoksina A
IS  - 120
SP  - 103
EP  - 110
DO  - 10.2298/ZMSPN1120103B
UR  - conv_57
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Salma, Nataša M. and Stanković, Slavica and Pantić, Vladimir R. and Dolić, Bisera J.",
year = "2011",
abstract = "Nine isolates of fungi of genera Aspergillus, Fusarium, Paecilomyces and Penicillium were cultured on the modified Vogel's medium with the addition of crude ochratoxin A (OTA) extract. This crude OTA extract was derived from a natural solid substrate on which Aspergillus ochraceus strain CBS 108.08 was cultivated. OTA was isolated, partially purified, dried by evaporating and dissolved in ethanol (1 mg ml-1), and added to the test medium up to the final concentration of 10 μg ml-1. The presence of OTA residues was determined after 7 and 14 day cultivation of fungi in the test medium at 27±1°C. The Paecilomyces lilacinus isolate (Inf. 2/A), which completely degraded OTA (150 μg) after only seven days, was selected for further studies. Wet sterile rice grains (50 g + 25 ml distilled water) were inoculated with individual isolates of fungi A. ochraceus (strain CBS 108.08) and P. lilacinus (isolate Inf. 2/A), and with their combination. In the case of P. lilacinus monoculture, 0.9 mg of crude OTA was also added into cultivation substrate. Each test was done in three replications. After the four week cultivation of individual and combined fungi at 27±1°C, inoculated rice grains were dried to the constant weight and pulverized. OTA was determined in these samples by the application of standard TLC method for fodder analysis. OTA in the amount of 61.310 μg kg-1 dry matter (DM) was determined only in the samples inoculated with a producer of ochratoxin A (A. ochraceus, strain CBS 108.08). On the other hand, a much smaller amount of OTA (80 μg kg-1 DM) was detected in samples inoculated with combined cultures of A. ochraceus and P. lilacinus isolates. Gained results indicate that P. lilacinus degraded, on average, 99.8% of OTA. After four week cultivation, the same fungal isolate in the samples of wet sterile rice kernels with the addition of 0.9 mg of crude OTA, completely degraded added crude OTA ( lt 8 μg kg-1)., Devet izolata gljiva iz rodova Aspergillus, Fusarium, Paecilomyces i Penicillium gajeno je na modifikovanoj Vogelovoj podlozi sa dodatkom sirovog ekstrakta ohratoksina A (OTA). Sirovi ekstrakt OTA je dobijen iz čvrstog prirodnog supstrata na kojem je gajen soj Aspergillus ochraceus CBS 108.08. Izolovan i delimično prečišćen OTA, uparen do suvog ostatka i rastvoren u etanolu (1 mg ml-1), dodat je u test podlogu do finalne koncentracije 10 μg ml-1. Nakon sedam i 14 dana gajenja kultura gljiva u test podlozi na 27 ± 1°C de terminisano je prisustvo rezidua OTA primenom modifikovane metode Filtenborg-a i sar. (1983). Od devet testiranih izolata za dalja ispitivanja je odabran izolat Paecilomyces lilacinus (Inf. 2/A), koji je već posle sedam dana u potpunosti razgradio inicijalnu količinu OTA (150 μg). U drugom delu eksperimenta vlažno sterilno zrno pirinča (50 g + 25 ml destilovane vode) zasejano je sa pojedinačnim izolatima A. ochraceus (CBS 108.08) i P. lilacinus (Inf. 2-A), kao i kombinacijom oba izolata. U slučaju mono-kulture P. lilacinus u podlogu je dodat i sirovi OTA (0,9 mg). Svaki od testova je urađen u 3 ponavljanja. Nakon četiri nedelje gajenja monokultura i mešanih kul tura gljiva na 27±1°C, inokulisana zrna su osušena do konstantne težine i sa mlevena do finog praha. U ovim uzorcima izvršena je determinacija OTA primenom standardne metode tankoslojne hromatografije za analizu stočne hrane. U uzorcima koji su bili zasejani samo sa producentom OTA (A. ochraceus, soj CBS 108.08) detektovan je OTA u prosečnoj količini od 61.310 μg kg-1 suvog ostatka. U uzorcima koji su bili zasejani kombinovanim kulturama izolata A. ochraceus i P. lilacinus utvrđena je znatno manja prosečna količina OTA (80 μg kg-1). Ovi rezultati ukazuju da je izolat P. lilacinus razgradio prosečno 99,8% OTA prisutnog u podlozi za kultivaciju. U uzorcima vlažnog sterilnog zrna pirinča sa dodatkom 0,9 mg sirovog OTA isti gljivični izolat je posle četiri nedelje kultivacije kompletno biorazgradio dodat sirovi OTA ( lt 8 μg kg-1).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A, Uloga Paecilomyces lilacinus (Thom) Samson i drugih vrsta gljiva u biodegradaciji ohratoksina A",
number = "120",
pages = "103-110",
doi = "10.2298/ZMSPN1120103B",
url = "conv_57"
}
Bočarov-Stančić, A., Lević, J., Salma, N. M., Stanković, S., Pantić, V. R.,& Dolić, B. J.. (2011). The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(120), 103-110.
https://doi.org/10.2298/ZMSPN1120103B
conv_57
Bočarov-Stančić A, Lević J, Salma NM, Stanković S, Pantić VR, Dolić BJ. The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A. in Zbornik Matice srpske za prirodne nauke. 2011;(120):103-110.
doi:10.2298/ZMSPN1120103B
conv_57 .
Bočarov-Stančić, Aleksandra, Lević, Jelena, Salma, Nataša M., Stanković, Slavica, Pantić, Vladimir R., Dolić, Bisera J., "The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A" in Zbornik Matice srpske za prirodne nauke, no. 120 (2011):103-110,
https://doi.org/10.2298/ZMSPN1120103B .,
conv_57 .
1

In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate

Bočarov-Stančić, Aleksandra; Stanković, Slavica; Lević, Jelena; Salma, Nataša M.; Pantić, Vladimir R.; Barnić, Saša S.

(Matica srpska, Novi Sad, 2011)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Salma, Nataša M.
AU  - Pantić, Vladimir R.
AU  - Barnić, Saša S.
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/383
AB  - Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium. According to previously performed experiments it was proved that the selected isolate, originating from sunflower meal, had the ability to degrade these fusariotoxins when growing on the modified Vogel's agar supplemented with crude extracts of DAS and T-2 toxin. In order to determine biodegradation of fusariotoxins, the liquid nutritive medium - SPY (5% sucrose + 0.1% peptone + 0.1% yeast extract, pH 6.2) was simultaneously inoculated with the isolate M. racemosus f. racemosus 1215/09 and: a) Fusarium semitectum SL-B (DAS producer) or b) F. sporotrichioides R-2301 (T-2 toxin producer). The SPY media, inoculated with single fungal isolates, were used as a control of toxin biosynthesis. The cultures were incubated at room temperature (21-26ºC) on the rotary shaker (175 rpm). After the 3-5-day incubation, the filtration of liquid cultures and the extraction of fusariotoxins from filtrates with ethyl-acetate were performed. Determinations of DAS and T-2 toxin were done by thin layer chromatography using silica gel G. Depending on the incubation duration, M. racemosus f. racemosus in the mixed culture with F. semitectum degraded from 90.0 to 99.97% of DAS present in the medium (40,000- 120,000 µg l-1), while in the mixed culture with F. sporotrichioides it degraded from 95.0 to 96.7% of T-2 toxin present in the medium (240,000 µg l-1). Sterile filtrates of mixed cultures and single culture of M. racemosus f. racemosus, obtained by passing liquid cultures through the 0.45-µm membrane filter and added to the SPY medium, did not affect degradation of type A trichothecenes that had been biosynthesised by isolates F. semitectum SL-B and F. sporotrichioides R-2301 in the liquid medium.
AB  - U kontrolisanim in vitro uslovima proučavana je sposobnost izolata Mucor racemosus f. racemosus 1215/09 da degraduje trihotecene tipa A (diacetoksiscirpenol - DAS i T-2 toksin) u tečnoj hranljivoj podlozi. Prethodnim eksperimentima je dokazano da odabrani izolat, poreklom sa suncokretove sačme, poseduje sposobnost razgradnje navedenih fuzariotoksina, koji su kao sirovi ekstrakti dodati u modifikovanu Vogelovu podlogu. U cilju utvrđivanja biodegradacije fuzariotoksina tečna hranljiva podloga SPK (5% saharoza + 0,1% pepton + 0,1% ekstrakt kvasca, pH 6,2) je zasejana u isto vreme izolatom M. racemosus f. racemosus 1215/09 i: a) Fusarium semitectum SL-B (proizvođač DAS-a) ili b) F. sporotrichioides R-2301 (proizvođač T-2 toksina). Kao kontrola biosinteze toksina korišćena je SPK podloga inokulisana pojedinačnim izolatima gljiva. Kulture su inkubirane na rotacionoj tresilici (175 o/min) tokom 3-5 dana na sobnoj temperaturi (21-26ºC). Nakon 3 do 5 dana inkubacije vršeno je filtriranje tečnih kultura i ekstrakcija fuzariotoksina iz filtrata etil-acetatom. Determinacija DAS-a i T-2 toksina je rađena tankoslojnom hromatografijom na silika gelu G. Zavisno od dužine inkubacije, M. racemosus f. racemosus je u združenoj kulturi sa F. semitectum degradovala 90,0-99,97% DAS-a prisutnog u podlozi (40.000-120.000 µg l-1), dok je u združenoj kulturi sa F. sporotrichioides razgradila 95,0-96,7% T-2 toksina prisutnog u podlozi (240.000 µg l-1). Sterilni filtrati mešanih kultura i pojedinačne kulture M. racemosus f. racemosus, dobijeni propuštanjem tečnih kultura kroz 0,45 µm membranski filter i dodati SPK podlozi, nisu uticali na razgradnju trihotecena tipa A koje su biosintetisali izolati F. semitectum SL-B i F. sporotrichioides R-2301 u tečnoj podlozi.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate
T1  - In vitro degradacija diacetoksiscirpenola i T-2 toksina posredstvom izolata Mucor racemosus fresen. f. racemosus
IS  - 121
SP  - 51
EP  - 59
DO  - 10.2298/ZMSPN1121051B
UR  - conv_58
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Stanković, Slavica and Lević, Jelena and Salma, Nataša M. and Pantić, Vladimir R. and Barnić, Saša S.",
year = "2011",
abstract = "Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium. According to previously performed experiments it was proved that the selected isolate, originating from sunflower meal, had the ability to degrade these fusariotoxins when growing on the modified Vogel's agar supplemented with crude extracts of DAS and T-2 toxin. In order to determine biodegradation of fusariotoxins, the liquid nutritive medium - SPY (5% sucrose + 0.1% peptone + 0.1% yeast extract, pH 6.2) was simultaneously inoculated with the isolate M. racemosus f. racemosus 1215/09 and: a) Fusarium semitectum SL-B (DAS producer) or b) F. sporotrichioides R-2301 (T-2 toxin producer). The SPY media, inoculated with single fungal isolates, were used as a control of toxin biosynthesis. The cultures were incubated at room temperature (21-26ºC) on the rotary shaker (175 rpm). After the 3-5-day incubation, the filtration of liquid cultures and the extraction of fusariotoxins from filtrates with ethyl-acetate were performed. Determinations of DAS and T-2 toxin were done by thin layer chromatography using silica gel G. Depending on the incubation duration, M. racemosus f. racemosus in the mixed culture with F. semitectum degraded from 90.0 to 99.97% of DAS present in the medium (40,000- 120,000 µg l-1), while in the mixed culture with F. sporotrichioides it degraded from 95.0 to 96.7% of T-2 toxin present in the medium (240,000 µg l-1). Sterile filtrates of mixed cultures and single culture of M. racemosus f. racemosus, obtained by passing liquid cultures through the 0.45-µm membrane filter and added to the SPY medium, did not affect degradation of type A trichothecenes that had been biosynthesised by isolates F. semitectum SL-B and F. sporotrichioides R-2301 in the liquid medium., U kontrolisanim in vitro uslovima proučavana je sposobnost izolata Mucor racemosus f. racemosus 1215/09 da degraduje trihotecene tipa A (diacetoksiscirpenol - DAS i T-2 toksin) u tečnoj hranljivoj podlozi. Prethodnim eksperimentima je dokazano da odabrani izolat, poreklom sa suncokretove sačme, poseduje sposobnost razgradnje navedenih fuzariotoksina, koji su kao sirovi ekstrakti dodati u modifikovanu Vogelovu podlogu. U cilju utvrđivanja biodegradacije fuzariotoksina tečna hranljiva podloga SPK (5% saharoza + 0,1% pepton + 0,1% ekstrakt kvasca, pH 6,2) je zasejana u isto vreme izolatom M. racemosus f. racemosus 1215/09 i: a) Fusarium semitectum SL-B (proizvođač DAS-a) ili b) F. sporotrichioides R-2301 (proizvođač T-2 toksina). Kao kontrola biosinteze toksina korišćena je SPK podloga inokulisana pojedinačnim izolatima gljiva. Kulture su inkubirane na rotacionoj tresilici (175 o/min) tokom 3-5 dana na sobnoj temperaturi (21-26ºC). Nakon 3 do 5 dana inkubacije vršeno je filtriranje tečnih kultura i ekstrakcija fuzariotoksina iz filtrata etil-acetatom. Determinacija DAS-a i T-2 toksina je rađena tankoslojnom hromatografijom na silika gelu G. Zavisno od dužine inkubacije, M. racemosus f. racemosus je u združenoj kulturi sa F. semitectum degradovala 90,0-99,97% DAS-a prisutnog u podlozi (40.000-120.000 µg l-1), dok je u združenoj kulturi sa F. sporotrichioides razgradila 95,0-96,7% T-2 toksina prisutnog u podlozi (240.000 µg l-1). Sterilni filtrati mešanih kultura i pojedinačne kulture M. racemosus f. racemosus, dobijeni propuštanjem tečnih kultura kroz 0,45 µm membranski filter i dodati SPK podlozi, nisu uticali na razgradnju trihotecena tipa A koje su biosintetisali izolati F. semitectum SL-B i F. sporotrichioides R-2301 u tečnoj podlozi.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate, In vitro degradacija diacetoksiscirpenola i T-2 toksina posredstvom izolata Mucor racemosus fresen. f. racemosus",
number = "121",
pages = "51-59",
doi = "10.2298/ZMSPN1121051B",
url = "conv_58"
}
Bočarov-Stančić, A., Stanković, S., Lević, J., Salma, N. M., Pantić, V. R.,& Barnić, S. S.. (2011). In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(121), 51-59.
https://doi.org/10.2298/ZMSPN1121051B
conv_58
Bočarov-Stančić A, Stanković S, Lević J, Salma NM, Pantić VR, Barnić SS. In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate. in Zbornik Matice srpske za prirodne nauke. 2011;(121):51-59.
doi:10.2298/ZMSPN1121051B
conv_58 .
Bočarov-Stančić, Aleksandra, Stanković, Slavica, Lević, Jelena, Salma, Nataša M., Pantić, Vladimir R., Barnić, Saša S., "In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate" in Zbornik Matice srpske za prirodne nauke, no. 121 (2011):51-59,
https://doi.org/10.2298/ZMSPN1121051B .,
conv_58 .
2

Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions

Bočarov-Stančić, Aleksandra; Lević, Jelena; Stanković, Slavica; Stanišić, Mladen M.; Bilek, Saša O.

(Matica srpska, Novi Sad, 2009)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Stanišić, Mladen M.
AU  - Bilek, Saša O.
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/255
AB  - Toxicological investigations encompassed two cultures of Fusarium graminearum: (i) D2 isolate, originating from air was obtained on Sabouraud medium during a routine control of laboratory sterility conditions at the Department of Microbiology of the Center for Bio-Ecology in 2006, and (ii) GZ-LES control isolate, a well known producer of zearalenone (ZON) and deoxynivalenol (DON), was isolated from maize kernel collected at Leskovac in 1975. Preliminary analysis of fungal potential for the production of DON and ZON were performed by the modified rapid screening method of Filtenborg et al. (1983). Dynamics of DON and ZON biosynthesis was tested under different conditions of isolate cultivation: (i) in a basic liquid semi-synthetic medium with 2% yeast extract and 15% sucrose, pH 6.5 (YES), (ii) in broth with same concentrations of yeast extract and sucrose supplemented with 0.23 mg/l ZnSO4 x 5 H2O, pH 6.5 (YESZn) and (iii) on natural solid substrates such as wet sterilized maize and rice kernels. The quantitative determination of DON and ZON was performed in both liquid and natural solid substrates with thin-layer chromatographic methods (TLCs). The maximum yield of DON was recorded after three weeks of cultivation on maize kernels at 27±1°C. Contrary to the D2 isolate, which did not show the potential for the DON biosynthesis, the control isolate GZ-LES produced 645.6 ppb of the same type B trichothecene under previously mentioned conditions. The ZON biosynthesis by the isolate D2 (1.2 ppb) was observed after 2 weeks of the stationary cultivation in YES and YESZn at room temperature (17-19°C). The same isolate produced 0.74 ppb and 17.35 ppb ZON on maize and rice kernels after only 7 and 28 days of cultivation at the room temperature ranging from 17 to 19°C and from 15 to 23°C, respectively.
AB  - Ispitivanjem su bili obuhvaćeni novoizolovana kultura F. graminearum (D2) neispitanog toksikološkog profila i kontrolni izolat iste vrste gljive (GZ-LES) dobro poznat proizvođač deoksinivalenola (DON) i zearalenona (ZON). Preliminarne analize DON-a i ZON-a su izvršene prema modifikovanoj metodi Filtenborga i sar. (1983). Dinamika biosinteze DON-a i ZON-a je praćena gajenjem izolata gljiva u/na četiri različita tipa podloge: tečnoj polusintetičkoj podlozi sa 2% ekstrakta kvasca i 15% saharoze (YES pH 6,5), podlozi istog sastava sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O (YESZn pH 6,5) i sterilisanim vlažnim zrnima kukuruza i pirinča. Kvantitativno određivanje DON-a u kulturi izolata gljiva gajenih u tečnoj podlozi je izvršeno tankoslojnom hromatografijom prema Cvetniću i sar. (2005), a u čvrstoj podlozi primenom postupka autora Pepeljnjaka i Babića (1991). Potencijal za biosintezu ZON-a kod izolata gajenih u tečnoj podlozi je određivan postupkom tankoslojne hromatografije prema Bočarov - Stančić i sar. (2003), a izolata gajenih na čvrstoj podlozi prema Balzeru i sar. (1978). Maksimalna koncentracija DON-a (645,6 ppb) je determinisana posle tri nedelje kultivacije kontrolnog izolata F. graminearum GZ-LES na zrnu kukuruza i pri 27±1°S. Za izolat iste vrste gljive D2 je utvrđeno da ne poseduje sposobnost biosinteze DON-a, s obzirom da nije proizvodio ovaj trihotecen tipa B ni u jednom od testiranih uslova kultivacije. Proizvodnja ZON-a je konstatovana kod izolata D2 (1,2 ppb, odnosno 1,5 ppb) posle dve nedelje stacionarne kultivacije u tečnim podlogama (YES i YESZn) na sobnoj temperaturi od 17 do 19°C. Na prirodnim čvrstim supstratima (zrno kukuruza i pirinča) isti izolat je biosintetisao ZON već posle sedam dana kultivacije na zrnu kukuruza i sobnoj temperaturi od 17 do 19°C (0,74 ppb) ili posle 28 dana kultivacije na zrnu pirinča i sobnoj temperaturi od 15 do 23°C (17,4 ppb).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions
T1  - Dinamika proizvodnje deoksinivalenola i zearalenona kod izolata Fusarium graminearum u laboratorijskim uslovima
IS  - 116
SP  - 15
EP  - 24
DO  - 10.2298/ZMSPN0916015B
UR  - conv_50
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Stanković, Slavica and Stanišić, Mladen M. and Bilek, Saša O.",
year = "2009",
abstract = "Toxicological investigations encompassed two cultures of Fusarium graminearum: (i) D2 isolate, originating from air was obtained on Sabouraud medium during a routine control of laboratory sterility conditions at the Department of Microbiology of the Center for Bio-Ecology in 2006, and (ii) GZ-LES control isolate, a well known producer of zearalenone (ZON) and deoxynivalenol (DON), was isolated from maize kernel collected at Leskovac in 1975. Preliminary analysis of fungal potential for the production of DON and ZON were performed by the modified rapid screening method of Filtenborg et al. (1983). Dynamics of DON and ZON biosynthesis was tested under different conditions of isolate cultivation: (i) in a basic liquid semi-synthetic medium with 2% yeast extract and 15% sucrose, pH 6.5 (YES), (ii) in broth with same concentrations of yeast extract and sucrose supplemented with 0.23 mg/l ZnSO4 x 5 H2O, pH 6.5 (YESZn) and (iii) on natural solid substrates such as wet sterilized maize and rice kernels. The quantitative determination of DON and ZON was performed in both liquid and natural solid substrates with thin-layer chromatographic methods (TLCs). The maximum yield of DON was recorded after three weeks of cultivation on maize kernels at 27±1°C. Contrary to the D2 isolate, which did not show the potential for the DON biosynthesis, the control isolate GZ-LES produced 645.6 ppb of the same type B trichothecene under previously mentioned conditions. The ZON biosynthesis by the isolate D2 (1.2 ppb) was observed after 2 weeks of the stationary cultivation in YES and YESZn at room temperature (17-19°C). The same isolate produced 0.74 ppb and 17.35 ppb ZON on maize and rice kernels after only 7 and 28 days of cultivation at the room temperature ranging from 17 to 19°C and from 15 to 23°C, respectively., Ispitivanjem su bili obuhvaćeni novoizolovana kultura F. graminearum (D2) neispitanog toksikološkog profila i kontrolni izolat iste vrste gljive (GZ-LES) dobro poznat proizvođač deoksinivalenola (DON) i zearalenona (ZON). Preliminarne analize DON-a i ZON-a su izvršene prema modifikovanoj metodi Filtenborga i sar. (1983). Dinamika biosinteze DON-a i ZON-a je praćena gajenjem izolata gljiva u/na četiri različita tipa podloge: tečnoj polusintetičkoj podlozi sa 2% ekstrakta kvasca i 15% saharoze (YES pH 6,5), podlozi istog sastava sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O (YESZn pH 6,5) i sterilisanim vlažnim zrnima kukuruza i pirinča. Kvantitativno određivanje DON-a u kulturi izolata gljiva gajenih u tečnoj podlozi je izvršeno tankoslojnom hromatografijom prema Cvetniću i sar. (2005), a u čvrstoj podlozi primenom postupka autora Pepeljnjaka i Babića (1991). Potencijal za biosintezu ZON-a kod izolata gajenih u tečnoj podlozi je određivan postupkom tankoslojne hromatografije prema Bočarov - Stančić i sar. (2003), a izolata gajenih na čvrstoj podlozi prema Balzeru i sar. (1978). Maksimalna koncentracija DON-a (645,6 ppb) je determinisana posle tri nedelje kultivacije kontrolnog izolata F. graminearum GZ-LES na zrnu kukuruza i pri 27±1°S. Za izolat iste vrste gljive D2 je utvrđeno da ne poseduje sposobnost biosinteze DON-a, s obzirom da nije proizvodio ovaj trihotecen tipa B ni u jednom od testiranih uslova kultivacije. Proizvodnja ZON-a je konstatovana kod izolata D2 (1,2 ppb, odnosno 1,5 ppb) posle dve nedelje stacionarne kultivacije u tečnim podlogama (YES i YESZn) na sobnoj temperaturi od 17 do 19°C. Na prirodnim čvrstim supstratima (zrno kukuruza i pirinča) isti izolat je biosintetisao ZON već posle sedam dana kultivacije na zrnu kukuruza i sobnoj temperaturi od 17 do 19°C (0,74 ppb) ili posle 28 dana kultivacije na zrnu pirinča i sobnoj temperaturi od 15 do 23°C (17,4 ppb).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions, Dinamika proizvodnje deoksinivalenola i zearalenona kod izolata Fusarium graminearum u laboratorijskim uslovima",
number = "116",
pages = "15-24",
doi = "10.2298/ZMSPN0916015B",
url = "conv_50"
}
Bočarov-Stančić, A., Lević, J., Stanković, S., Stanišić, M. M.,& Bilek, S. O.. (2009). Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(116), 15-24.
https://doi.org/10.2298/ZMSPN0916015B
conv_50
Bočarov-Stančić A, Lević J, Stanković S, Stanišić MM, Bilek SO. Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions. in Zbornik Matice srpske za prirodne nauke. 2009;(116):15-24.
doi:10.2298/ZMSPN0916015B
conv_50 .
Bočarov-Stančić, Aleksandra, Lević, Jelena, Stanković, Slavica, Stanišić, Mladen M., Bilek, Saša O., "Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions" in Zbornik Matice srpske za prirodne nauke, no. 116 (2009):15-24,
https://doi.org/10.2298/ZMSPN0916015B .,
conv_50 .
2

Fusarium species: The occurrence and the importance in agriculture of Serbia

Lević, Jelena; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra

(Matica srpska, Novi Sad, 2009)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/267
AB  - Fusarium species have been isolated from over 100 plant species in Serbia. From the economic aspect, they have been and still are the most important for the production and storage of small grains and maize, and are exceptionally important for some other species. Total of 63 species, 35 varieties (var.) and 19 specialised forms (f. sp.) of basic species, particularly of F. oxysporum (4 var. and 12 f. sp.) and F. solani (7 var. and 3 f. sp.) were identified. Species F. langsethiae and F. thapsinum, recently identified, have been isolated from wheat and s o r g h u m seeds, respectively. F. graminearum is the most important pathogen for wheat, barely and maize, while F. poae is also important for wheat and barely. Furthermore, species of the section Liseola (F. verticillioides, F. subglutinans and F. proliferatum) are important for maize and sorghum. In recent years, species of the section Liseola have been increasingly occurring in wheat and barley. The June-October period in Serbia is the most critical period for quality maintenance of stored maize, as the abundance and frequency of fungi, particularly of toxigenic species of the genus Fusarium, are the greatest during that period. In general, there is a lack of data about fusarioses of industrial crops in Serbia. There are mere descriptions of specific cases in which the development of Fusarium species was mostly emphasised by agroecological conditions. The presence of recently determined Fusarium species in kernels of these plant species indicates their importance from the aspect of the yield reduction and grain quality debasement and the mycotoxin contamination. Root rot and plant wilt are characteristic symptoms of fusarioses for forage and vegetable crops, while pathological changes in fruits provoked by Fusarium species are less frequent. F. oxysporum and its specialised forms prevail in these plant species.
AB  - Vrste roda Fusarium su u Srbiji izolovane sa preko 100 biljnih vrsta, a sa ekonomskog aspekta, bile i ostale, najznačajnije za proizvodnju i čuvanje strnih žita i kukuruza. Samo izuzetno su značajne i za neke druge biljne vrste. Ukupno je do sada identifikovano: 63 vrste, 35 varijeteta (var.) i 19 specijalizovanih formi (f. sp.) osnovnih vrsta, posebno vrste F. oxysporum (4 var. i 12 f. sp.) i F. solani (7 var. i 3 f. sp.). F. langsethiae i F. thapsinum su novoidentifikovane vrste izolovane sa zrna pšenice, odnosno sirka. F. graminearum je najznačajniji patogen pšenice, ječma i kukuruza, a F. poae i patogen pšenice i ječma. Vrste iz sekcije Liseola (F. verticillioides, F. subglutinans i F. proliferatum) značajni su i patogeni kukuruza i siraka, mada je poslednjih godina utvrđena sve češća pojava ovih vrsta na zrnu pšenice i ječma. U Srbiji je period jun-oktobar najkritičniji za očuvanje kvaliteta uskladištenog kukuruza jer su brojnost i učestalost gljiva u tom periodu najveće, posebno toksigenih vrsta roda Fusarium (43,5-62,5%). Generalno, fuzarioze industrijskih biljaka su malo proučavane u Srbiji. Opisi su samo pojedinačni slučajevi u kojima je razvoj Fusarium vrsta najčešće potenciran povoljnim agroklimatskih uslovima. Prisustvo Fusarium vrsta na semenu industrijskih biljaka, koje je poslednjih godina sve češće utvrđeno, ukazuje da ove patogene gljive u Srbiji mogu biti značajne sa stanovišta smanjenja prinosa i kvaliteta zrna, posebno u pogledu kontaminiranosti mikotoksinima. Trulež korena i uvenulost biljaka su karakteristični simptomi fuzarioza krmnih i povrtarskih biljaka, dok je pojava patoloških promena na plodovima ređa. Na ovim biljnim vrstama dominiraju F. oxysporum i njene specijalizovane forme.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Fusarium species: The occurrence and the importance in agriculture of Serbia
T1  - Fusarium vrste - pojava i značaj u Srbiji
IS  - 116
SP  - 33
EP  - 48
DO  - 10.2298/ZMSPN0916033L
UR  - conv_52
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra",
year = "2009",
abstract = "Fusarium species have been isolated from over 100 plant species in Serbia. From the economic aspect, they have been and still are the most important for the production and storage of small grains and maize, and are exceptionally important for some other species. Total of 63 species, 35 varieties (var.) and 19 specialised forms (f. sp.) of basic species, particularly of F. oxysporum (4 var. and 12 f. sp.) and F. solani (7 var. and 3 f. sp.) were identified. Species F. langsethiae and F. thapsinum, recently identified, have been isolated from wheat and s o r g h u m seeds, respectively. F. graminearum is the most important pathogen for wheat, barely and maize, while F. poae is also important for wheat and barely. Furthermore, species of the section Liseola (F. verticillioides, F. subglutinans and F. proliferatum) are important for maize and sorghum. In recent years, species of the section Liseola have been increasingly occurring in wheat and barley. The June-October period in Serbia is the most critical period for quality maintenance of stored maize, as the abundance and frequency of fungi, particularly of toxigenic species of the genus Fusarium, are the greatest during that period. In general, there is a lack of data about fusarioses of industrial crops in Serbia. There are mere descriptions of specific cases in which the development of Fusarium species was mostly emphasised by agroecological conditions. The presence of recently determined Fusarium species in kernels of these plant species indicates their importance from the aspect of the yield reduction and grain quality debasement and the mycotoxin contamination. Root rot and plant wilt are characteristic symptoms of fusarioses for forage and vegetable crops, while pathological changes in fruits provoked by Fusarium species are less frequent. F. oxysporum and its specialised forms prevail in these plant species., Vrste roda Fusarium su u Srbiji izolovane sa preko 100 biljnih vrsta, a sa ekonomskog aspekta, bile i ostale, najznačajnije za proizvodnju i čuvanje strnih žita i kukuruza. Samo izuzetno su značajne i za neke druge biljne vrste. Ukupno je do sada identifikovano: 63 vrste, 35 varijeteta (var.) i 19 specijalizovanih formi (f. sp.) osnovnih vrsta, posebno vrste F. oxysporum (4 var. i 12 f. sp.) i F. solani (7 var. i 3 f. sp.). F. langsethiae i F. thapsinum su novoidentifikovane vrste izolovane sa zrna pšenice, odnosno sirka. F. graminearum je najznačajniji patogen pšenice, ječma i kukuruza, a F. poae i patogen pšenice i ječma. Vrste iz sekcije Liseola (F. verticillioides, F. subglutinans i F. proliferatum) značajni su i patogeni kukuruza i siraka, mada je poslednjih godina utvrđena sve češća pojava ovih vrsta na zrnu pšenice i ječma. U Srbiji je period jun-oktobar najkritičniji za očuvanje kvaliteta uskladištenog kukuruza jer su brojnost i učestalost gljiva u tom periodu najveće, posebno toksigenih vrsta roda Fusarium (43,5-62,5%). Generalno, fuzarioze industrijskih biljaka su malo proučavane u Srbiji. Opisi su samo pojedinačni slučajevi u kojima je razvoj Fusarium vrsta najčešće potenciran povoljnim agroklimatskih uslovima. Prisustvo Fusarium vrsta na semenu industrijskih biljaka, koje je poslednjih godina sve češće utvrđeno, ukazuje da ove patogene gljive u Srbiji mogu biti značajne sa stanovišta smanjenja prinosa i kvaliteta zrna, posebno u pogledu kontaminiranosti mikotoksinima. Trulež korena i uvenulost biljaka su karakteristični simptomi fuzarioza krmnih i povrtarskih biljaka, dok je pojava patoloških promena na plodovima ređa. Na ovim biljnim vrstama dominiraju F. oxysporum i njene specijalizovane forme.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Fusarium species: The occurrence and the importance in agriculture of Serbia, Fusarium vrste - pojava i značaj u Srbiji",
number = "116",
pages = "33-48",
doi = "10.2298/ZMSPN0916033L",
url = "conv_52"
}
Lević, J., Stanković, S., Krnjaja, V.,& Bočarov-Stančić, A.. (2009). Fusarium species: The occurrence and the importance in agriculture of Serbia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(116), 33-48.
https://doi.org/10.2298/ZMSPN0916033L
conv_52
Lević J, Stanković S, Krnjaja V, Bočarov-Stančić A. Fusarium species: The occurrence and the importance in agriculture of Serbia. in Zbornik Matice srpske za prirodne nauke. 2009;(116):33-48.
doi:10.2298/ZMSPN0916033L
conv_52 .
Lević, Jelena, Stanković, Slavica, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, "Fusarium species: The occurrence and the importance in agriculture of Serbia" in Zbornik Matice srpske za prirodne nauke, no. 116 (2009):33-48,
https://doi.org/10.2298/ZMSPN0916033L .,
conv_52 .
19

Investigation of toxigenic potential of fungal species by the use of simple screening method

Bočarov-Stančić, Aleksandra; Lević, Jelena; Dimić, Gordana R.; Stanković, Slavica; Salma, Nataša M.

(Matica srpska, Novi Sad, 2009)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Dimić, Gordana R.
AU  - Stanković, Slavica
AU  - Salma, Nataša M.
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/281
AB  - Potential for the biosynthesis of aflatoxin B1 (AFLB1), ochratoxin A (OTA), diacetoxyscirpenol (DAS), T-2 toxin (T2), and zearalenone (ZON) was investigated in different fungal species belonging to the genera: Aspergillus, Fusarium and Penicillium. The majority of investigated isolates originated from cereal grains, crushed oil soybean seed and fodder mixtures. The simple screening method developed by Filtenborg et al. (1983) was applied with few modifications concerning the type of the medium and cultivation temperature. In order to optimise the biosynthetic conditions for different mycotoxins, the following control cultures, known as mycotin producers were used: OTA - A. ochraceus CBS 108.08, DAS - F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) and ZON - F. graminearum (GZ-LES). The fungi were cultivated on the standard medium (YESA - 2% yeast extract, 15% sucrose and 2% agar, pH 6.5), three modifications of the basic medium (YESAZn - the standard medium supplemented with 0.23 mg/l ZnSO4 x 5 H2O; PPSA - the medium in which yeast extract was replaced with peptone-1; PPSAZn - the medium in which yeast extract was replaced with peptone-1 and supplemented with 0.23 mg/l ZnSO4 x 5 H2O), and the potato-dextrose agar (PDA). The earlier biosynthesis of tested mycotoxins was recorded under the following cultivation conditions of fungal species: AFLB1 - after 14 days on PDA at 27±1°C, OTA - after 10 days on YESA and YESAZn at 27±1°C, DAS - after 10 days on PPSA and PPSAZn at 27±1°C, T2 - after 7 days on PPSAZn and PPSA at room temperature (20-24°C), and ZON - after 1 week on YESA and YESAZn at room temperature (21-24°C).
AB  - Potencijal za biosintezu aflatoksina B1 (AFLB1), ohratoksina A (OTA), diacetoksiscirpenola (DAS), T-2 toksina (T2) i zearalenona je ispitan kod izolata gljiva koje pripadaju rodovima Aspergillus, Fusarium i Penicillium. Izolati su uglavnom bili poreklom sa zrna žitarica i stočne hrane. Primenjena je jednostavna trijažna metoda F i l t e n b o r g-a i sar. (1983) kod koje su izvršene izvesne modifikacije u smislu tipa podloge i temperature kultivacije gljiva. U cilju optimizacije uslova za testiranje toksigenog profila odabranih gljivičnih izolata upotrebljene su kontrolne kulture za koje je prethodno dokazano da su proizvođači sledećih mikotoksina: OTA-a A. ochraceus CBS 108.08, DAS-a F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) i ZON-a F. graminearum (GZ-LES). Gljive su gajene na standardnoj podlozi (EKSA - 2% ekstrakta kvasca i 15% saharoze, pH 6,5), tri modifikacije osnovne podloge (EKSAZn-standardna podloga sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O; PPSA - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1; PPSAZn - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1 i kojoj je dodato 0,23 mg/l ZnSO4 x 5 H2O; pH 6,5) i krompir dekstroznoj podlozi (pH 6,5). Biosinteza ispitanih mikotoksina je najranije konstatovana pri sledećim uslovima gajenja gljiva: AFLB1 - posle 14 dana kultivacije na KDA i 27±1°C, OTA - posle 10 dana kultivacije na EKSA i EKSAZn i 27±1°C, DAS-a posle 10 dana kultivacije na PPSA i PPSAZn na 27±1°C, T2 - posle 7 dana na PPSA i PPSAZn i sobnoj temperaturi (20-24°C), i ZON-a posle nedelju dana na EKSA i EKSAZn i sobnoj temperaturi (21-24°C).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Investigation of toxigenic potential of fungal species by the use of simple screening method
T1  - Ispitivanje toksigenog potencijala gljiva primenom jednostavnog trijažnog metoda
IS  - 116
SP  - 25
EP  - 32
DO  - 10.2298/ZMSPN0916025B
UR  - conv_51
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Dimić, Gordana R. and Stanković, Slavica and Salma, Nataša M.",
year = "2009",
abstract = "Potential for the biosynthesis of aflatoxin B1 (AFLB1), ochratoxin A (OTA), diacetoxyscirpenol (DAS), T-2 toxin (T2), and zearalenone (ZON) was investigated in different fungal species belonging to the genera: Aspergillus, Fusarium and Penicillium. The majority of investigated isolates originated from cereal grains, crushed oil soybean seed and fodder mixtures. The simple screening method developed by Filtenborg et al. (1983) was applied with few modifications concerning the type of the medium and cultivation temperature. In order to optimise the biosynthetic conditions for different mycotoxins, the following control cultures, known as mycotin producers were used: OTA - A. ochraceus CBS 108.08, DAS - F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) and ZON - F. graminearum (GZ-LES). The fungi were cultivated on the standard medium (YESA - 2% yeast extract, 15% sucrose and 2% agar, pH 6.5), three modifications of the basic medium (YESAZn - the standard medium supplemented with 0.23 mg/l ZnSO4 x 5 H2O; PPSA - the medium in which yeast extract was replaced with peptone-1; PPSAZn - the medium in which yeast extract was replaced with peptone-1 and supplemented with 0.23 mg/l ZnSO4 x 5 H2O), and the potato-dextrose agar (PDA). The earlier biosynthesis of tested mycotoxins was recorded under the following cultivation conditions of fungal species: AFLB1 - after 14 days on PDA at 27±1°C, OTA - after 10 days on YESA and YESAZn at 27±1°C, DAS - after 10 days on PPSA and PPSAZn at 27±1°C, T2 - after 7 days on PPSAZn and PPSA at room temperature (20-24°C), and ZON - after 1 week on YESA and YESAZn at room temperature (21-24°C)., Potencijal za biosintezu aflatoksina B1 (AFLB1), ohratoksina A (OTA), diacetoksiscirpenola (DAS), T-2 toksina (T2) i zearalenona je ispitan kod izolata gljiva koje pripadaju rodovima Aspergillus, Fusarium i Penicillium. Izolati su uglavnom bili poreklom sa zrna žitarica i stočne hrane. Primenjena je jednostavna trijažna metoda F i l t e n b o r g-a i sar. (1983) kod koje su izvršene izvesne modifikacije u smislu tipa podloge i temperature kultivacije gljiva. U cilju optimizacije uslova za testiranje toksigenog profila odabranih gljivičnih izolata upotrebljene su kontrolne kulture za koje je prethodno dokazano da su proizvođači sledećih mikotoksina: OTA-a A. ochraceus CBS 108.08, DAS-a F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) i ZON-a F. graminearum (GZ-LES). Gljive su gajene na standardnoj podlozi (EKSA - 2% ekstrakta kvasca i 15% saharoze, pH 6,5), tri modifikacije osnovne podloge (EKSAZn-standardna podloga sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O; PPSA - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1; PPSAZn - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1 i kojoj je dodato 0,23 mg/l ZnSO4 x 5 H2O; pH 6,5) i krompir dekstroznoj podlozi (pH 6,5). Biosinteza ispitanih mikotoksina je najranije konstatovana pri sledećim uslovima gajenja gljiva: AFLB1 - posle 14 dana kultivacije na KDA i 27±1°C, OTA - posle 10 dana kultivacije na EKSA i EKSAZn i 27±1°C, DAS-a posle 10 dana kultivacije na PPSA i PPSAZn na 27±1°C, T2 - posle 7 dana na PPSA i PPSAZn i sobnoj temperaturi (20-24°C), i ZON-a posle nedelju dana na EKSA i EKSAZn i sobnoj temperaturi (21-24°C).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Investigation of toxigenic potential of fungal species by the use of simple screening method, Ispitivanje toksigenog potencijala gljiva primenom jednostavnog trijažnog metoda",
number = "116",
pages = "25-32",
doi = "10.2298/ZMSPN0916025B",
url = "conv_51"
}
Bočarov-Stančić, A., Lević, J., Dimić, G. R., Stanković, S.,& Salma, N. M.. (2009). Investigation of toxigenic potential of fungal species by the use of simple screening method. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(116), 25-32.
https://doi.org/10.2298/ZMSPN0916025B
conv_51
Bočarov-Stančić A, Lević J, Dimić GR, Stanković S, Salma NM. Investigation of toxigenic potential of fungal species by the use of simple screening method. in Zbornik Matice srpske za prirodne nauke. 2009;(116):25-32.
doi:10.2298/ZMSPN0916025B
conv_51 .
Bočarov-Stančić, Aleksandra, Lević, Jelena, Dimić, Gordana R., Stanković, Slavica, Salma, Nataša M., "Investigation of toxigenic potential of fungal species by the use of simple screening method" in Zbornik Matice srpske za prirodne nauke, no. 116 (2009):25-32,
https://doi.org/10.2298/ZMSPN0916025B .,
conv_51 .
7

Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings

Lević, Jelena; Stanković, Slavica; Kovačević, Tamara; Tančić, Sonja; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra

(Cereal Res Inst, Szeged, 2008)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Kovačević, Tamara
AU  - Tančić, Sonja
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/243
AB  - Effects of nine isolates of F. langsethiae, originating from Serbia (2005-2006), on vigour of wheat seedlings were the objective of this study. Symptoms of seedlings root rot, reduced of seed germination and retardation of coleoptiles and roots growth were observed. Growth of seedlings roots treated with the filtrate of isolates was reduced in comparison to control. Isolates of F. langsethiae were separated into three VCGs.
PB  - Cereal Res Inst, Szeged
T2  - Cereal Research Communications
T1  - Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings
VL  - 36
SP  - 515
EP  - 516
DO  - 10.1556/CRC.36.2008.Suppl.B.43
UR  - conv_713
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Kovačević, Tamara and Tančić, Sonja and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra",
year = "2008",
abstract = "Effects of nine isolates of F. langsethiae, originating from Serbia (2005-2006), on vigour of wheat seedlings were the objective of this study. Symptoms of seedlings root rot, reduced of seed germination and retardation of coleoptiles and roots growth were observed. Growth of seedlings roots treated with the filtrate of isolates was reduced in comparison to control. Isolates of F. langsethiae were separated into three VCGs.",
publisher = "Cereal Res Inst, Szeged",
journal = "Cereal Research Communications",
title = "Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings",
volume = "36",
pages = "515-516",
doi = "10.1556/CRC.36.2008.Suppl.B.43",
url = "conv_713"
}
Lević, J., Stanković, S., Kovačević, T., Tančić, S., Krnjaja, V.,& Bočarov-Stančić, A.. (2008). Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings. in Cereal Research Communications
Cereal Res Inst, Szeged., 36, 515-516.
https://doi.org/10.1556/CRC.36.2008.Suppl.B.43
conv_713
Lević J, Stanković S, Kovačević T, Tančić S, Krnjaja V, Bočarov-Stančić A. Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings. in Cereal Research Communications. 2008;36:515-516.
doi:10.1556/CRC.36.2008.Suppl.B.43
conv_713 .
Lević, Jelena, Stanković, Slavica, Kovačević, Tamara, Tančić, Sonja, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, "Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings" in Cereal Research Communications, 36 (2008):515-516,
https://doi.org/10.1556/CRC.36.2008.Suppl.B.43 .,
conv_713 .
1
1
1

Fusarium head blight and grain yield losses of wheat in Serbia

Lević, Jelena; Stanković, Slavica; Ivanović, Dragica; Kovačević, Tamara; Tančić, Sonja; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra

(Cereal Res Inst, Szeged, 2008)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Ivanović, Dragica
AU  - Kovačević, Tamara
AU  - Tančić, Sonja
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/242
AB  - The objective of this study was to identify crop production factor, associated with FHB development in Serbian wheat. FHB index and weight losses, varying from 16% to 74% and from 1.8% to 38.3%, respectively, indicated that enviromental conditions were the most important factor affecting disease development and yield losses. F. graminearum dominated on kernels (tip to 55.5%) and whole rachis (34.7%), while F poae was predominant on apical part od rachis (35.2%).
PB  - Cereal Res Inst, Szeged
T2  - Cereal Research Communications
T1  - Fusarium head blight and grain yield losses of wheat in Serbia
VL  - 36
SP  - 513
EP  - 514
DO  - 10.1556/CRC.36.2008.Suppl.B.43
UR  - conv_712
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Ivanović, Dragica and Kovačević, Tamara and Tančić, Sonja and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra",
year = "2008",
abstract = "The objective of this study was to identify crop production factor, associated with FHB development in Serbian wheat. FHB index and weight losses, varying from 16% to 74% and from 1.8% to 38.3%, respectively, indicated that enviromental conditions were the most important factor affecting disease development and yield losses. F. graminearum dominated on kernels (tip to 55.5%) and whole rachis (34.7%), while F poae was predominant on apical part od rachis (35.2%).",
publisher = "Cereal Res Inst, Szeged",
journal = "Cereal Research Communications",
title = "Fusarium head blight and grain yield losses of wheat in Serbia",
volume = "36",
pages = "513-514",
doi = "10.1556/CRC.36.2008.Suppl.B.43",
url = "conv_712"
}
Lević, J., Stanković, S., Ivanović, D., Kovačević, T., Tančić, S., Krnjaja, V.,& Bočarov-Stančić, A.. (2008). Fusarium head blight and grain yield losses of wheat in Serbia. in Cereal Research Communications
Cereal Res Inst, Szeged., 36, 513-514.
https://doi.org/10.1556/CRC.36.2008.Suppl.B.43
conv_712
Lević J, Stanković S, Ivanović D, Kovačević T, Tančić S, Krnjaja V, Bočarov-Stančić A. Fusarium head blight and grain yield losses of wheat in Serbia. in Cereal Research Communications. 2008;36:513-514.
doi:10.1556/CRC.36.2008.Suppl.B.43
conv_712 .
Lević, Jelena, Stanković, Slavica, Ivanović, Dragica, Kovačević, Tamara, Tančić, Sonja, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, "Fusarium head blight and grain yield losses of wheat in Serbia" in Cereal Research Communications, 36 (2008):513-514,
https://doi.org/10.1556/CRC.36.2008.Suppl.B.43 .,
conv_712 .
1
4
4

Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels

Bočarov-Stančić, Aleksandra; Lević, Jelena; Stanković, Slavica; Tančić, Sonja; Krnjaja, Vesna; Salma, Nataša M.

(Akademiai Kiado Zrt, Budapest, 2008)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Tančić, Sonja
AU  - Krnjaja, Vesna
AU  - Salma, Nataša M.
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/238
AB  - The toxigenic potential of F. langsethiae cultures isolated from Serbian wheat kernels harvested in 2005 was investigated. In vitro experiments were performed at room temperature (24-28 degrees C) with two different media: liquid GPYE and wet( sterilized wheat grain. All of the tested F. langsethiae isolates produced T-2 toxin (0.312 - 48.0 ppm) and DAS (0.3 12-12.0 ppm), but only one (MRIZP-1208) zearalenone.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Cereal Research Communications
T1  - Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels
VL  - 36
SP  - 345
EP  - 346
DO  - 10.1556/CRC.36.2008.Suppl.B.33
UR  - conv_708
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Stanković, Slavica and Tančić, Sonja and Krnjaja, Vesna and Salma, Nataša M.",
year = "2008",
abstract = "The toxigenic potential of F. langsethiae cultures isolated from Serbian wheat kernels harvested in 2005 was investigated. In vitro experiments were performed at room temperature (24-28 degrees C) with two different media: liquid GPYE and wet( sterilized wheat grain. All of the tested F. langsethiae isolates produced T-2 toxin (0.312 - 48.0 ppm) and DAS (0.3 12-12.0 ppm), but only one (MRIZP-1208) zearalenone.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Cereal Research Communications",
title = "Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels",
volume = "36",
pages = "345-346",
doi = "10.1556/CRC.36.2008.Suppl.B.33",
url = "conv_708"
}
Bočarov-Stančić, A., Lević, J., Stanković, S., Tančić, S., Krnjaja, V.,& Salma, N. M.. (2008). Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels. in Cereal Research Communications
Akademiai Kiado Zrt, Budapest., 36, 345-346.
https://doi.org/10.1556/CRC.36.2008.Suppl.B.33
conv_708
Bočarov-Stančić A, Lević J, Stanković S, Tančić S, Krnjaja V, Salma NM. Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels. in Cereal Research Communications. 2008;36:345-346.
doi:10.1556/CRC.36.2008.Suppl.B.33
conv_708 .
Bočarov-Stančić, Aleksandra, Lević, Jelena, Stanković, Slavica, Tančić, Sonja, Krnjaja, Vesna, Salma, Nataša M., "Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels" in Cereal Research Communications, 36 (2008):345-346,
https://doi.org/10.1556/CRC.36.2008.Suppl.B.33 .,
conv_708 .
1
5
6

Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia

Stanković, Slavica; Lević, Jelena; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra; Tančić, Sonja; Kovačević, Tamara

(Matica srpska, Novi Sad, 2007)

TY  - JOUR
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
AU  - Tančić, Sonja
AU  - Kovačević, Tamara
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/195
AB  - A total of 88 and 40 wheat samples collected immediately prior to harvest in 2005 and 2006, respectively, under different agroecological conditions, were studied in respect to the occurrence of Fusarium spp. and the production of fusariotoxins. The greatest number of samples was infected with species of the genera Fusarium (81.8 and 65.0%), and Alternaria (36.3 and 17.5%) with the intensity ranging from 9.4 to 84.0% in 2005 and from 23.4 to 80.6% in 2006. Out of 13 identified species belonging to the genus Fusarium, F. graminearum had the highest frequency (35.2 and 12.5%) and the intensity up to 67.2%, and 21.9%, in 2005 and 2006, respectively, followed by F. poae but only in 2005 (20.4%), and F. proliferatum in 2006 (19.7%). The natural occurrence of mycotoxins in positive samples varied from 37 to 331 ppb for zearalenone and from 31 to 125 ppb for diacetoxyscirpenol (DAS) and T-2 toxin. The concentration of mycotoxins amounted, on average, to 133.4, 61.0 and 45.7 ppb for zearelenone, DAS and T-2 toxin, respectively.
AB  - Osamdeset osam i 40 uzoraka pšenice, prikupljenih neposredno pred žetvu u 2005. i 2006. godini u različitim agroekološkim uslovima u Srbiji proučavani su radi praćenja pojave Fusarium spp. i stvaranja fuzariotoksina. Najveći broj uzoraka bio je zaražen vrstama roda Fusarium (81,8 i 65,0%), a zatim sa Alternaria spp. (36,3 i 17,5%) sa intenzitetom 9,4-84,0% u 2005. i 23,4-80,6% u 2006. godini. Od ukupno 13 identifikovanih vrsta iz roda Fusarium najučestalija je bila F. graminearum (35,2 i 12,5%) sa intenzitetom do 67,2% u 2005. i do 21,9% u 2006. godini, a zatim F. poae, ali samo u 2005. godini (20,4%), i F. proliferatum u 2006. godini (19.7%). Prirodna pojava mikotoksina u pozitivnim uzorcima je varirala od 37 do 331 ppb za zearalenon i od 31 do 125 ppb za diacetoksiscirpenol (DAS) i T-2 toksin. U proseku, koncentracija mikotoksina je bila 133,4 ppb za zearelonon, 61,0 ppb za DAS i 45,7 ppb za T-2 toksin.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia
T1  - Učestalost toksigenih vrsta Fusarium i fuzariotoksina u zrnu pšenice u Srbiji
IS  - 113
SP  - 93
EP  - 102
DO  - 10.2298/ZMSPN0713093S
UR  - conv_46
ER  - 
@article{
author = "Stanković, Slavica and Lević, Jelena and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra and Tančić, Sonja and Kovačević, Tamara",
year = "2007",
abstract = "A total of 88 and 40 wheat samples collected immediately prior to harvest in 2005 and 2006, respectively, under different agroecological conditions, were studied in respect to the occurrence of Fusarium spp. and the production of fusariotoxins. The greatest number of samples was infected with species of the genera Fusarium (81.8 and 65.0%), and Alternaria (36.3 and 17.5%) with the intensity ranging from 9.4 to 84.0% in 2005 and from 23.4 to 80.6% in 2006. Out of 13 identified species belonging to the genus Fusarium, F. graminearum had the highest frequency (35.2 and 12.5%) and the intensity up to 67.2%, and 21.9%, in 2005 and 2006, respectively, followed by F. poae but only in 2005 (20.4%), and F. proliferatum in 2006 (19.7%). The natural occurrence of mycotoxins in positive samples varied from 37 to 331 ppb for zearalenone and from 31 to 125 ppb for diacetoxyscirpenol (DAS) and T-2 toxin. The concentration of mycotoxins amounted, on average, to 133.4, 61.0 and 45.7 ppb for zearelenone, DAS and T-2 toxin, respectively., Osamdeset osam i 40 uzoraka pšenice, prikupljenih neposredno pred žetvu u 2005. i 2006. godini u različitim agroekološkim uslovima u Srbiji proučavani su radi praćenja pojave Fusarium spp. i stvaranja fuzariotoksina. Najveći broj uzoraka bio je zaražen vrstama roda Fusarium (81,8 i 65,0%), a zatim sa Alternaria spp. (36,3 i 17,5%) sa intenzitetom 9,4-84,0% u 2005. i 23,4-80,6% u 2006. godini. Od ukupno 13 identifikovanih vrsta iz roda Fusarium najučestalija je bila F. graminearum (35,2 i 12,5%) sa intenzitetom do 67,2% u 2005. i do 21,9% u 2006. godini, a zatim F. poae, ali samo u 2005. godini (20,4%), i F. proliferatum u 2006. godini (19.7%). Prirodna pojava mikotoksina u pozitivnim uzorcima je varirala od 37 do 331 ppb za zearalenon i od 31 do 125 ppb za diacetoksiscirpenol (DAS) i T-2 toksin. U proseku, koncentracija mikotoksina je bila 133,4 ppb za zearelonon, 61,0 ppb za DAS i 45,7 ppb za T-2 toksin.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia, Učestalost toksigenih vrsta Fusarium i fuzariotoksina u zrnu pšenice u Srbiji",
number = "113",
pages = "93-102",
doi = "10.2298/ZMSPN0713093S",
url = "conv_46"
}
Stanković, S., Lević, J., Krnjaja, V., Bočarov-Stančić, A., Tančić, S.,& Kovačević, T.. (2007). Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 93-102.
https://doi.org/10.2298/ZMSPN0713093S
conv_46
Stanković S, Lević J, Krnjaja V, Bočarov-Stančić A, Tančić S, Kovačević T. Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia. in Zbornik Matice srpske za prirodne nauke. 2007;(113):93-102.
doi:10.2298/ZMSPN0713093S
conv_46 .
Stanković, Slavica, Lević, Jelena, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, Tančić, Sonja, Kovačević, Tamara, "Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):93-102,
https://doi.org/10.2298/ZMSPN0713093S .,
conv_46 .
15

The toxigenic potential of Fusarium poae originated from wheat

Bočarov-Stančić, Aleksandra; Lević, Jelena; Stanković, Slavica; Krnjaja, Vesna; Kovačević, Tamara; Tančić, Sonja

(Matica srpska, Novi Sad, 2007)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Kovačević, Tamara
AU  - Tančić, Sonja
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/197
AB  - Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively.
AB  - U ovom radu je u in vitro uslovima proučena sposobnost izolata F. poae za biosintezu jedne grupe fuzariotoksina - trihotecena tipa A (T-2 toksin i diacetoksiscirpenol - DAS), kao i zearalenona (ZEA). Toksikološki profil ove vrste je kod nas nedovoljno ispitan s obzirom na njenu zastupljenost i toksigena svojstva prema literaturnim podacima. Proučavanjima je bilo obuhvaćeno 11 izolata F. poae, poreklom sa pšenice iz 9 lokaliteta, uglavnom sa područja Vojvodine. Najveći broj uzoraka prikupljen je 2005. godine, kada su klimatski uslovi pogodovali intenzivnijoj pojavi fuzarioza klasa ove poljoprivredne kulture. Za određivanje toksikološkog potencijala F. poae kulture odabranih izolata su gajene u tečnim podlogama (GPK i SPK) tokom 3 dana na sobnoj temperaturi (21-26°C) i na rotacionoj tresilici (180 obrtaja min-1). Sirovi toksini su izolovani iz filtrata tečnih kultura ispitanih izolata pomoću etil acetata, dok je kvantifikacija mikotoksina izvršena metodom tankoslojne hromatografije. Tečna kultura izolata GZ-LES (F. graminearum) je korišćena kao kontrolna kultura za utvrđivanje potencijala za biosintezu zearalenona, a KF-38/1/R (F. sporotrichioides) za oba trihotecena tipa A (T-2 toksin i DAS). Dobijeni rezultati pokazuju da F. poae, za razliku od F. graminearum, ne poseduje potencijal za biosintezu zearalenona. Prisustvo DAS-a je utvrđeno samo kod jedne kulture F. poae (MRIZP-666) i kontrolnog izolata F. sporotrichioides (KF-38/1/R) koji su gajeni u tečnom GPK medijumu. T-2 toksin je detektovan kod izolata MRIZP-666 pri gajenju u obema podlogama, kao i izolata MRIZP-37 u GPK, odnosno MRIZP-860 u SPK medijumu. Kontrolna kultura KF-38/1/R (F. sporotrichioides) proizvodila je T-2 toksin u koncentraciji od 4000 μg L-1. Na osnovu iznetih podataka može se zaključiti da je potencijal F. poae za biosintezu trihotecena tipa A bio nizak u datim uslovima s obzirom da koncentracija DAS-a nije prelazila 80 μg L-1, odnosno T-2 toksina 240 μg L-1. Imajući u vidu prikazane rezultate, smatramo da je za dobijanje konačnog odgovora na pitanje o toksikološkom profilu izolata F. poae u Srbiji neophodno preduzeti dodatna ispitivanja, ne samo sa novim izolatima iz godina koje slede, nego i u drugim uslovima kultivisanja, u prvom redu na sterilnom prirodnom supstratu kao što su pšenica i kukuruz.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - The toxigenic potential of Fusarium poae originated from wheat
T1  - Toksigeni potencijal izolata Fusarium poae poreklom sa pšenice
IS  - 113
SP  - 113
EP  - 123
DO  - 10.2298/ZMSPN0713113B
UR  - conv_48
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Stanković, Slavica and Krnjaja, Vesna and Kovačević, Tamara and Tančić, Sonja",
year = "2007",
abstract = "Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively., U ovom radu je u in vitro uslovima proučena sposobnost izolata F. poae za biosintezu jedne grupe fuzariotoksina - trihotecena tipa A (T-2 toksin i diacetoksiscirpenol - DAS), kao i zearalenona (ZEA). Toksikološki profil ove vrste je kod nas nedovoljno ispitan s obzirom na njenu zastupljenost i toksigena svojstva prema literaturnim podacima. Proučavanjima je bilo obuhvaćeno 11 izolata F. poae, poreklom sa pšenice iz 9 lokaliteta, uglavnom sa područja Vojvodine. Najveći broj uzoraka prikupljen je 2005. godine, kada su klimatski uslovi pogodovali intenzivnijoj pojavi fuzarioza klasa ove poljoprivredne kulture. Za određivanje toksikološkog potencijala F. poae kulture odabranih izolata su gajene u tečnim podlogama (GPK i SPK) tokom 3 dana na sobnoj temperaturi (21-26°C) i na rotacionoj tresilici (180 obrtaja min-1). Sirovi toksini su izolovani iz filtrata tečnih kultura ispitanih izolata pomoću etil acetata, dok je kvantifikacija mikotoksina izvršena metodom tankoslojne hromatografije. Tečna kultura izolata GZ-LES (F. graminearum) je korišćena kao kontrolna kultura za utvrđivanje potencijala za biosintezu zearalenona, a KF-38/1/R (F. sporotrichioides) za oba trihotecena tipa A (T-2 toksin i DAS). Dobijeni rezultati pokazuju da F. poae, za razliku od F. graminearum, ne poseduje potencijal za biosintezu zearalenona. Prisustvo DAS-a je utvrđeno samo kod jedne kulture F. poae (MRIZP-666) i kontrolnog izolata F. sporotrichioides (KF-38/1/R) koji su gajeni u tečnom GPK medijumu. T-2 toksin je detektovan kod izolata MRIZP-666 pri gajenju u obema podlogama, kao i izolata MRIZP-37 u GPK, odnosno MRIZP-860 u SPK medijumu. Kontrolna kultura KF-38/1/R (F. sporotrichioides) proizvodila je T-2 toksin u koncentraciji od 4000 μg L-1. Na osnovu iznetih podataka može se zaključiti da je potencijal F. poae za biosintezu trihotecena tipa A bio nizak u datim uslovima s obzirom da koncentracija DAS-a nije prelazila 80 μg L-1, odnosno T-2 toksina 240 μg L-1. Imajući u vidu prikazane rezultate, smatramo da je za dobijanje konačnog odgovora na pitanje o toksikološkom profilu izolata F. poae u Srbiji neophodno preduzeti dodatna ispitivanja, ne samo sa novim izolatima iz godina koje slede, nego i u drugim uslovima kultivisanja, u prvom redu na sterilnom prirodnom supstratu kao što su pšenica i kukuruz.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "The toxigenic potential of Fusarium poae originated from wheat, Toksigeni potencijal izolata Fusarium poae poreklom sa pšenice",
number = "113",
pages = "113-123",
doi = "10.2298/ZMSPN0713113B",
url = "conv_48"
}
Bočarov-Stančić, A., Lević, J., Stanković, S., Krnjaja, V., Kovačević, T.,& Tančić, S.. (2007). The toxigenic potential of Fusarium poae originated from wheat. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 113-123.
https://doi.org/10.2298/ZMSPN0713113B
conv_48
Bočarov-Stančić A, Lević J, Stanković S, Krnjaja V, Kovačević T, Tančić S. The toxigenic potential of Fusarium poae originated from wheat. in Zbornik Matice srpske za prirodne nauke. 2007;(113):113-123.
doi:10.2298/ZMSPN0713113B
conv_48 .
Bočarov-Stančić, Aleksandra, Lević, Jelena, Stanković, Slavica, Krnjaja, Vesna, Kovačević, Tamara, Tančić, Sonja, "The toxigenic potential of Fusarium poae originated from wheat" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):113-123,
https://doi.org/10.2298/ZMSPN0713113B .,
conv_48 .
2

Incidence and control of toxigenic fungi in stored cereals

Lević, Jelena; Stanković, Slavica; Bočarov-Stančić, Aleksandra

(Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2004)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Bočarov-Stančić, Aleksandra
PY  - 2004
UR  - http://rik.mrizp.rs/handle/123456789/73
AB  - Monitoring of the incidence of toxigenic species and the detection of mycotoxins in stored cereals (maize, wheat and barley) are of a special importance for agriculture in Serbia. The essential reason for this is the fact that cereals, as species most susceptible to toxigenic fungi, are cultivated on over 60% of arable land, then the incidence of potentially toxigenic fungi and natural occurrence of certain mycotoxins has been registered each year, while epizooties of mycotoxicoses have been detected in some years. Under agroecological conditions of Serbia, F. graminearum and F. verticillioides are the most frequent species - the first, on grain of both, maize and small grains, and the later, more on maize, while the third species F. sporotrichioides, belonging to the most toxic species in the world, especially in maize, is scarce. Besides, other numerous, potentially toxigenic Fusarium species, including: F. subglutinans, F. proliferatum, F. oxysporum, F. tricinctum, F. poae, F. culmorum, etc. have been detected. A frequent and calamitous incidence of European corn borer (Ostrinia nubilalis) is one of many factors that favor the intensive development of potentially toxic fungal species, especially of F. verticillioides and F. subglutinans. Other toxigenic species of fungi, such as species of the genera Aspergillus and Penicillium, are less important than the Fusarium species. According to previous studies in Serbia, the occurrence of aflatoxin B1 and ochratoxin A (Aspergillus spp. and Penicillium spp) zearaleonones and trichothecenes type A (T-2 toxin and diacetoxyscirpenol) (Fusarium spp) in cereals and their products was determined in concentrations even higher than tolerance values obligatory by the running regulations for food and feed. There are little data or none on other types of mycotoxins. Possibilities of maize artificial drying in Serbia are limited, especially in the case of small producers, which make over 80% of all producers. Therefore, maize is mainly dried naturally and is stored in cribs, which provides a further development of potentially toxigenic species and more intensive biosynthesis of certain mycotoxins. Cereal growers are generally recommended to apply preventive measures in order to reduce the incidence of potentially toxigenic genotypes and their metabolites, as well as, to grow more resistant and early maturity genotypes, to apply optimum cropping practices, harvest at full maturity, separate ears with pathological changes and frequently perform control during winter and early spring. On the other hand, in the industrial animal growing, the use of preparations for adsorption of mycotoxins already synthesized in cereals such as Min-a-zel Plus, is recommended in the preventive concentrations of 0.2% or 0.4% in the case when higher concentrations of mycotoxins are detected in cereals or commercial mixed feed.
AB  - Sadržaj vlage, temperatura, stepen zaraze zrna pre skladištenja, prisustvo insekata, količina polomljenog zrna, prisustvo stranih materija, način sušenja i dužina čuvanja, glavni su činioci koji utiču da li će uskladištena žita (kukuruz, pšenica, ječam i ostale strnine) biti kontaminirana toksigenim gljivama i mikotoksinima. U nas su Fusarium vrste potencijalno najznačajnije toksigene vrste, a zatim vrste iz rodova Aspergillus i Penicillium. U žitima i njihovim prerađevinama utvrđena je prirodna pojava aflatoksina B1 i ohratoksina A (Aspergillus spp. i Penicillium spp) zearalenona i trihotecena tipa A (T-2 toksin i diacetoksiscirpenol) (Fusarium spp), čak i u većim koncentracijama nego što je to dozvoljeno prema našim važećim pravilnicima za ishranu ljudi i životinja. Proizvođačima žita, uglavnom, se preporučuju preventivne mere za redukciju pojave potencijalno toksigenih vrsta i njihovih sekundarnih metabolita, kako u poljskim uslovima, tako i tokom čuvanja.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Incidence and control of toxigenic fungi in stored cereals
T1  - Pojava i suzbijanje toksigenih vrsta gljiva u uskladištenom žitu
VL  - 32
IS  - 3-4
SP  - 245
EP  - 254
UR  - conv_322
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Bočarov-Stančić, Aleksandra",
year = "2004",
abstract = "Monitoring of the incidence of toxigenic species and the detection of mycotoxins in stored cereals (maize, wheat and barley) are of a special importance for agriculture in Serbia. The essential reason for this is the fact that cereals, as species most susceptible to toxigenic fungi, are cultivated on over 60% of arable land, then the incidence of potentially toxigenic fungi and natural occurrence of certain mycotoxins has been registered each year, while epizooties of mycotoxicoses have been detected in some years. Under agroecological conditions of Serbia, F. graminearum and F. verticillioides are the most frequent species - the first, on grain of both, maize and small grains, and the later, more on maize, while the third species F. sporotrichioides, belonging to the most toxic species in the world, especially in maize, is scarce. Besides, other numerous, potentially toxigenic Fusarium species, including: F. subglutinans, F. proliferatum, F. oxysporum, F. tricinctum, F. poae, F. culmorum, etc. have been detected. A frequent and calamitous incidence of European corn borer (Ostrinia nubilalis) is one of many factors that favor the intensive development of potentially toxic fungal species, especially of F. verticillioides and F. subglutinans. Other toxigenic species of fungi, such as species of the genera Aspergillus and Penicillium, are less important than the Fusarium species. According to previous studies in Serbia, the occurrence of aflatoxin B1 and ochratoxin A (Aspergillus spp. and Penicillium spp) zearaleonones and trichothecenes type A (T-2 toxin and diacetoxyscirpenol) (Fusarium spp) in cereals and their products was determined in concentrations even higher than tolerance values obligatory by the running regulations for food and feed. There are little data or none on other types of mycotoxins. Possibilities of maize artificial drying in Serbia are limited, especially in the case of small producers, which make over 80% of all producers. Therefore, maize is mainly dried naturally and is stored in cribs, which provides a further development of potentially toxigenic species and more intensive biosynthesis of certain mycotoxins. Cereal growers are generally recommended to apply preventive measures in order to reduce the incidence of potentially toxigenic genotypes and their metabolites, as well as, to grow more resistant and early maturity genotypes, to apply optimum cropping practices, harvest at full maturity, separate ears with pathological changes and frequently perform control during winter and early spring. On the other hand, in the industrial animal growing, the use of preparations for adsorption of mycotoxins already synthesized in cereals such as Min-a-zel Plus, is recommended in the preventive concentrations of 0.2% or 0.4% in the case when higher concentrations of mycotoxins are detected in cereals or commercial mixed feed., Sadržaj vlage, temperatura, stepen zaraze zrna pre skladištenja, prisustvo insekata, količina polomljenog zrna, prisustvo stranih materija, način sušenja i dužina čuvanja, glavni su činioci koji utiču da li će uskladištena žita (kukuruz, pšenica, ječam i ostale strnine) biti kontaminirana toksigenim gljivama i mikotoksinima. U nas su Fusarium vrste potencijalno najznačajnije toksigene vrste, a zatim vrste iz rodova Aspergillus i Penicillium. U žitima i njihovim prerađevinama utvrđena je prirodna pojava aflatoksina B1 i ohratoksina A (Aspergillus spp. i Penicillium spp) zearalenona i trihotecena tipa A (T-2 toksin i diacetoksiscirpenol) (Fusarium spp), čak i u većim koncentracijama nego što je to dozvoljeno prema našim važećim pravilnicima za ishranu ljudi i životinja. Proizvođačima žita, uglavnom, se preporučuju preventivne mere za redukciju pojave potencijalno toksigenih vrsta i njihovih sekundarnih metabolita, kako u poljskim uslovima, tako i tokom čuvanja.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Incidence and control of toxigenic fungi in stored cereals, Pojava i suzbijanje toksigenih vrsta gljiva u uskladištenom žitu",
volume = "32",
number = "3-4",
pages = "245-254",
url = "conv_322"
}
Lević, J., Stanković, S.,& Bočarov-Stančić, A.. (2004). Incidence and control of toxigenic fungi in stored cereals. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 32(3-4), 245-254.
conv_322
Lević J, Stanković S, Bočarov-Stančić A. Incidence and control of toxigenic fungi in stored cereals. in Biljni lekar. 2004;32(3-4):245-254.
conv_322 .
Lević, Jelena, Stanković, Slavica, Bočarov-Stančić, Aleksandra, "Incidence and control of toxigenic fungi in stored cereals" in Biljni lekar, 32, no. 3-4 (2004):245-254,
conv_322 .