Salma, Nataša M.

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The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A

Bočarov-Stančić, Aleksandra; Lević, Jelena; Salma, Nataša M.; Stanković, Slavica; Pantić, Vladimir R.; Dolić, Bisera J.

(Matica srpska, Novi Sad, 2011)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Salma, Nataša M.
AU  - Stanković, Slavica
AU  - Pantić, Vladimir R.
AU  - Dolić, Bisera J.
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/386
AB  - Nine isolates of fungi of genera Aspergillus, Fusarium, Paecilomyces and Penicillium were cultured on the modified Vogel's medium with the addition of crude ochratoxin A (OTA) extract. This crude OTA extract was derived from a natural solid substrate on which Aspergillus ochraceus strain CBS 108.08 was cultivated. OTA was isolated, partially purified, dried by evaporating and dissolved in ethanol (1 mg ml-1), and added to the test medium up to the final concentration of 10 μg ml-1. The presence of OTA residues was determined after 7 and 14 day cultivation of fungi in the test medium at 27±1°C. The Paecilomyces lilacinus isolate (Inf. 2/A), which completely degraded OTA (150 μg) after only seven days, was selected for further studies. Wet sterile rice grains (50 g + 25 ml distilled water) were inoculated with individual isolates of fungi A. ochraceus (strain CBS 108.08) and P. lilacinus (isolate Inf. 2/A), and with their combination. In the case of P. lilacinus monoculture, 0.9 mg of crude OTA was also added into cultivation substrate. Each test was done in three replications. After the four week cultivation of individual and combined fungi at 27±1°C, inoculated rice grains were dried to the constant weight and pulverized. OTA was determined in these samples by the application of standard TLC method for fodder analysis. OTA in the amount of 61.310 μg kg-1 dry matter (DM) was determined only in the samples inoculated with a producer of ochratoxin A (A. ochraceus, strain CBS 108.08). On the other hand, a much smaller amount of OTA (80 μg kg-1 DM) was detected in samples inoculated with combined cultures of A. ochraceus and P. lilacinus isolates. Gained results indicate that P. lilacinus degraded, on average, 99.8% of OTA. After four week cultivation, the same fungal isolate in the samples of wet sterile rice kernels with the addition of 0.9 mg of crude OTA, completely degraded added crude OTA ( lt 8 μg kg-1).
AB  - Devet izolata gljiva iz rodova Aspergillus, Fusarium, Paecilomyces i Penicillium gajeno je na modifikovanoj Vogelovoj podlozi sa dodatkom sirovog ekstrakta ohratoksina A (OTA). Sirovi ekstrakt OTA je dobijen iz čvrstog prirodnog supstrata na kojem je gajen soj Aspergillus ochraceus CBS 108.08. Izolovan i delimično prečišćen OTA, uparen do suvog ostatka i rastvoren u etanolu (1 mg ml-1), dodat je u test podlogu do finalne koncentracije 10 μg ml-1. Nakon sedam i 14 dana gajenja kultura gljiva u test podlozi na 27 ± 1°C de terminisano je prisustvo rezidua OTA primenom modifikovane metode Filtenborg-a i sar. (1983). Od devet testiranih izolata za dalja ispitivanja je odabran izolat Paecilomyces lilacinus (Inf. 2/A), koji je već posle sedam dana u potpunosti razgradio inicijalnu količinu OTA (150 μg). U drugom delu eksperimenta vlažno sterilno zrno pirinča (50 g + 25 ml destilovane vode) zasejano je sa pojedinačnim izolatima A. ochraceus (CBS 108.08) i P. lilacinus (Inf. 2-A), kao i kombinacijom oba izolata. U slučaju mono-kulture P. lilacinus u podlogu je dodat i sirovi OTA (0,9 mg). Svaki od testova je urađen u 3 ponavljanja. Nakon četiri nedelje gajenja monokultura i mešanih kul tura gljiva na 27±1°C, inokulisana zrna su osušena do konstantne težine i sa mlevena do finog praha. U ovim uzorcima izvršena je determinacija OTA primenom standardne metode tankoslojne hromatografije za analizu stočne hrane. U uzorcima koji su bili zasejani samo sa producentom OTA (A. ochraceus, soj CBS 108.08) detektovan je OTA u prosečnoj količini od 61.310 μg kg-1 suvog ostatka. U uzorcima koji su bili zasejani kombinovanim kulturama izolata A. ochraceus i P. lilacinus utvrđena je znatno manja prosečna količina OTA (80 μg kg-1). Ovi rezultati ukazuju da je izolat P. lilacinus razgradio prosečno 99,8% OTA prisutnog u podlozi za kultivaciju. U uzorcima vlažnog sterilnog zrna pirinča sa dodatkom 0,9 mg sirovog OTA isti gljivični izolat je posle četiri nedelje kultivacije kompletno biorazgradio dodat sirovi OTA ( lt 8 μg kg-1).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A
T1  - Uloga Paecilomyces lilacinus (Thom) Samson i drugih vrsta gljiva u biodegradaciji ohratoksina A
IS  - 120
SP  - 103
EP  - 110
DO  - 10.2298/ZMSPN1120103B
UR  - conv_57
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Salma, Nataša M. and Stanković, Slavica and Pantić, Vladimir R. and Dolić, Bisera J.",
year = "2011",
abstract = "Nine isolates of fungi of genera Aspergillus, Fusarium, Paecilomyces and Penicillium were cultured on the modified Vogel's medium with the addition of crude ochratoxin A (OTA) extract. This crude OTA extract was derived from a natural solid substrate on which Aspergillus ochraceus strain CBS 108.08 was cultivated. OTA was isolated, partially purified, dried by evaporating and dissolved in ethanol (1 mg ml-1), and added to the test medium up to the final concentration of 10 μg ml-1. The presence of OTA residues was determined after 7 and 14 day cultivation of fungi in the test medium at 27±1°C. The Paecilomyces lilacinus isolate (Inf. 2/A), which completely degraded OTA (150 μg) after only seven days, was selected for further studies. Wet sterile rice grains (50 g + 25 ml distilled water) were inoculated with individual isolates of fungi A. ochraceus (strain CBS 108.08) and P. lilacinus (isolate Inf. 2/A), and with their combination. In the case of P. lilacinus monoculture, 0.9 mg of crude OTA was also added into cultivation substrate. Each test was done in three replications. After the four week cultivation of individual and combined fungi at 27±1°C, inoculated rice grains were dried to the constant weight and pulverized. OTA was determined in these samples by the application of standard TLC method for fodder analysis. OTA in the amount of 61.310 μg kg-1 dry matter (DM) was determined only in the samples inoculated with a producer of ochratoxin A (A. ochraceus, strain CBS 108.08). On the other hand, a much smaller amount of OTA (80 μg kg-1 DM) was detected in samples inoculated with combined cultures of A. ochraceus and P. lilacinus isolates. Gained results indicate that P. lilacinus degraded, on average, 99.8% of OTA. After four week cultivation, the same fungal isolate in the samples of wet sterile rice kernels with the addition of 0.9 mg of crude OTA, completely degraded added crude OTA ( lt 8 μg kg-1)., Devet izolata gljiva iz rodova Aspergillus, Fusarium, Paecilomyces i Penicillium gajeno je na modifikovanoj Vogelovoj podlozi sa dodatkom sirovog ekstrakta ohratoksina A (OTA). Sirovi ekstrakt OTA je dobijen iz čvrstog prirodnog supstrata na kojem je gajen soj Aspergillus ochraceus CBS 108.08. Izolovan i delimično prečišćen OTA, uparen do suvog ostatka i rastvoren u etanolu (1 mg ml-1), dodat je u test podlogu do finalne koncentracije 10 μg ml-1. Nakon sedam i 14 dana gajenja kultura gljiva u test podlozi na 27 ± 1°C de terminisano je prisustvo rezidua OTA primenom modifikovane metode Filtenborg-a i sar. (1983). Od devet testiranih izolata za dalja ispitivanja je odabran izolat Paecilomyces lilacinus (Inf. 2/A), koji je već posle sedam dana u potpunosti razgradio inicijalnu količinu OTA (150 μg). U drugom delu eksperimenta vlažno sterilno zrno pirinča (50 g + 25 ml destilovane vode) zasejano je sa pojedinačnim izolatima A. ochraceus (CBS 108.08) i P. lilacinus (Inf. 2-A), kao i kombinacijom oba izolata. U slučaju mono-kulture P. lilacinus u podlogu je dodat i sirovi OTA (0,9 mg). Svaki od testova je urađen u 3 ponavljanja. Nakon četiri nedelje gajenja monokultura i mešanih kul tura gljiva na 27±1°C, inokulisana zrna su osušena do konstantne težine i sa mlevena do finog praha. U ovim uzorcima izvršena je determinacija OTA primenom standardne metode tankoslojne hromatografije za analizu stočne hrane. U uzorcima koji su bili zasejani samo sa producentom OTA (A. ochraceus, soj CBS 108.08) detektovan je OTA u prosečnoj količini od 61.310 μg kg-1 suvog ostatka. U uzorcima koji su bili zasejani kombinovanim kulturama izolata A. ochraceus i P. lilacinus utvrđena je znatno manja prosečna količina OTA (80 μg kg-1). Ovi rezultati ukazuju da je izolat P. lilacinus razgradio prosečno 99,8% OTA prisutnog u podlozi za kultivaciju. U uzorcima vlažnog sterilnog zrna pirinča sa dodatkom 0,9 mg sirovog OTA isti gljivični izolat je posle četiri nedelje kultivacije kompletno biorazgradio dodat sirovi OTA ( lt 8 μg kg-1).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A, Uloga Paecilomyces lilacinus (Thom) Samson i drugih vrsta gljiva u biodegradaciji ohratoksina A",
number = "120",
pages = "103-110",
doi = "10.2298/ZMSPN1120103B",
url = "conv_57"
}
Bočarov-Stančić, A., Lević, J., Salma, N. M., Stanković, S., Pantić, V. R.,& Dolić, B. J.. (2011). The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(120), 103-110.
https://doi.org/10.2298/ZMSPN1120103B
conv_57
Bočarov-Stančić A, Lević J, Salma NM, Stanković S, Pantić VR, Dolić BJ. The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A. in Zbornik Matice srpske za prirodne nauke. 2011;(120):103-110.
doi:10.2298/ZMSPN1120103B
conv_57 .
Bočarov-Stančić, Aleksandra, Lević, Jelena, Salma, Nataša M., Stanković, Slavica, Pantić, Vladimir R., Dolić, Bisera J., "The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A" in Zbornik Matice srpske za prirodne nauke, no. 120 (2011):103-110,
https://doi.org/10.2298/ZMSPN1120103B .,
conv_57 .
1

In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate

Bočarov-Stančić, Aleksandra; Stanković, Slavica; Lević, Jelena; Salma, Nataša M.; Pantić, Vladimir R.; Barnić, Saša S.

(Matica srpska, Novi Sad, 2011)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Salma, Nataša M.
AU  - Pantić, Vladimir R.
AU  - Barnić, Saša S.
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/383
AB  - Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium. According to previously performed experiments it was proved that the selected isolate, originating from sunflower meal, had the ability to degrade these fusariotoxins when growing on the modified Vogel's agar supplemented with crude extracts of DAS and T-2 toxin. In order to determine biodegradation of fusariotoxins, the liquid nutritive medium - SPY (5% sucrose + 0.1% peptone + 0.1% yeast extract, pH 6.2) was simultaneously inoculated with the isolate M. racemosus f. racemosus 1215/09 and: a) Fusarium semitectum SL-B (DAS producer) or b) F. sporotrichioides R-2301 (T-2 toxin producer). The SPY media, inoculated with single fungal isolates, were used as a control of toxin biosynthesis. The cultures were incubated at room temperature (21-26ºC) on the rotary shaker (175 rpm). After the 3-5-day incubation, the filtration of liquid cultures and the extraction of fusariotoxins from filtrates with ethyl-acetate were performed. Determinations of DAS and T-2 toxin were done by thin layer chromatography using silica gel G. Depending on the incubation duration, M. racemosus f. racemosus in the mixed culture with F. semitectum degraded from 90.0 to 99.97% of DAS present in the medium (40,000- 120,000 µg l-1), while in the mixed culture with F. sporotrichioides it degraded from 95.0 to 96.7% of T-2 toxin present in the medium (240,000 µg l-1). Sterile filtrates of mixed cultures and single culture of M. racemosus f. racemosus, obtained by passing liquid cultures through the 0.45-µm membrane filter and added to the SPY medium, did not affect degradation of type A trichothecenes that had been biosynthesised by isolates F. semitectum SL-B and F. sporotrichioides R-2301 in the liquid medium.
AB  - U kontrolisanim in vitro uslovima proučavana je sposobnost izolata Mucor racemosus f. racemosus 1215/09 da degraduje trihotecene tipa A (diacetoksiscirpenol - DAS i T-2 toksin) u tečnoj hranljivoj podlozi. Prethodnim eksperimentima je dokazano da odabrani izolat, poreklom sa suncokretove sačme, poseduje sposobnost razgradnje navedenih fuzariotoksina, koji su kao sirovi ekstrakti dodati u modifikovanu Vogelovu podlogu. U cilju utvrđivanja biodegradacije fuzariotoksina tečna hranljiva podloga SPK (5% saharoza + 0,1% pepton + 0,1% ekstrakt kvasca, pH 6,2) je zasejana u isto vreme izolatom M. racemosus f. racemosus 1215/09 i: a) Fusarium semitectum SL-B (proizvođač DAS-a) ili b) F. sporotrichioides R-2301 (proizvođač T-2 toksina). Kao kontrola biosinteze toksina korišćena je SPK podloga inokulisana pojedinačnim izolatima gljiva. Kulture su inkubirane na rotacionoj tresilici (175 o/min) tokom 3-5 dana na sobnoj temperaturi (21-26ºC). Nakon 3 do 5 dana inkubacije vršeno je filtriranje tečnih kultura i ekstrakcija fuzariotoksina iz filtrata etil-acetatom. Determinacija DAS-a i T-2 toksina je rađena tankoslojnom hromatografijom na silika gelu G. Zavisno od dužine inkubacije, M. racemosus f. racemosus je u združenoj kulturi sa F. semitectum degradovala 90,0-99,97% DAS-a prisutnog u podlozi (40.000-120.000 µg l-1), dok je u združenoj kulturi sa F. sporotrichioides razgradila 95,0-96,7% T-2 toksina prisutnog u podlozi (240.000 µg l-1). Sterilni filtrati mešanih kultura i pojedinačne kulture M. racemosus f. racemosus, dobijeni propuštanjem tečnih kultura kroz 0,45 µm membranski filter i dodati SPK podlozi, nisu uticali na razgradnju trihotecena tipa A koje su biosintetisali izolati F. semitectum SL-B i F. sporotrichioides R-2301 u tečnoj podlozi.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate
T1  - In vitro degradacija diacetoksiscirpenola i T-2 toksina posredstvom izolata Mucor racemosus fresen. f. racemosus
IS  - 121
SP  - 51
EP  - 59
DO  - 10.2298/ZMSPN1121051B
UR  - conv_58
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Stanković, Slavica and Lević, Jelena and Salma, Nataša M. and Pantić, Vladimir R. and Barnić, Saša S.",
year = "2011",
abstract = "Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium. According to previously performed experiments it was proved that the selected isolate, originating from sunflower meal, had the ability to degrade these fusariotoxins when growing on the modified Vogel's agar supplemented with crude extracts of DAS and T-2 toxin. In order to determine biodegradation of fusariotoxins, the liquid nutritive medium - SPY (5% sucrose + 0.1% peptone + 0.1% yeast extract, pH 6.2) was simultaneously inoculated with the isolate M. racemosus f. racemosus 1215/09 and: a) Fusarium semitectum SL-B (DAS producer) or b) F. sporotrichioides R-2301 (T-2 toxin producer). The SPY media, inoculated with single fungal isolates, were used as a control of toxin biosynthesis. The cultures were incubated at room temperature (21-26ºC) on the rotary shaker (175 rpm). After the 3-5-day incubation, the filtration of liquid cultures and the extraction of fusariotoxins from filtrates with ethyl-acetate were performed. Determinations of DAS and T-2 toxin were done by thin layer chromatography using silica gel G. Depending on the incubation duration, M. racemosus f. racemosus in the mixed culture with F. semitectum degraded from 90.0 to 99.97% of DAS present in the medium (40,000- 120,000 µg l-1), while in the mixed culture with F. sporotrichioides it degraded from 95.0 to 96.7% of T-2 toxin present in the medium (240,000 µg l-1). Sterile filtrates of mixed cultures and single culture of M. racemosus f. racemosus, obtained by passing liquid cultures through the 0.45-µm membrane filter and added to the SPY medium, did not affect degradation of type A trichothecenes that had been biosynthesised by isolates F. semitectum SL-B and F. sporotrichioides R-2301 in the liquid medium., U kontrolisanim in vitro uslovima proučavana je sposobnost izolata Mucor racemosus f. racemosus 1215/09 da degraduje trihotecene tipa A (diacetoksiscirpenol - DAS i T-2 toksin) u tečnoj hranljivoj podlozi. Prethodnim eksperimentima je dokazano da odabrani izolat, poreklom sa suncokretove sačme, poseduje sposobnost razgradnje navedenih fuzariotoksina, koji su kao sirovi ekstrakti dodati u modifikovanu Vogelovu podlogu. U cilju utvrđivanja biodegradacije fuzariotoksina tečna hranljiva podloga SPK (5% saharoza + 0,1% pepton + 0,1% ekstrakt kvasca, pH 6,2) je zasejana u isto vreme izolatom M. racemosus f. racemosus 1215/09 i: a) Fusarium semitectum SL-B (proizvođač DAS-a) ili b) F. sporotrichioides R-2301 (proizvođač T-2 toksina). Kao kontrola biosinteze toksina korišćena je SPK podloga inokulisana pojedinačnim izolatima gljiva. Kulture su inkubirane na rotacionoj tresilici (175 o/min) tokom 3-5 dana na sobnoj temperaturi (21-26ºC). Nakon 3 do 5 dana inkubacije vršeno je filtriranje tečnih kultura i ekstrakcija fuzariotoksina iz filtrata etil-acetatom. Determinacija DAS-a i T-2 toksina je rađena tankoslojnom hromatografijom na silika gelu G. Zavisno od dužine inkubacije, M. racemosus f. racemosus je u združenoj kulturi sa F. semitectum degradovala 90,0-99,97% DAS-a prisutnog u podlozi (40.000-120.000 µg l-1), dok je u združenoj kulturi sa F. sporotrichioides razgradila 95,0-96,7% T-2 toksina prisutnog u podlozi (240.000 µg l-1). Sterilni filtrati mešanih kultura i pojedinačne kulture M. racemosus f. racemosus, dobijeni propuštanjem tečnih kultura kroz 0,45 µm membranski filter i dodati SPK podlozi, nisu uticali na razgradnju trihotecena tipa A koje su biosintetisali izolati F. semitectum SL-B i F. sporotrichioides R-2301 u tečnoj podlozi.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate, In vitro degradacija diacetoksiscirpenola i T-2 toksina posredstvom izolata Mucor racemosus fresen. f. racemosus",
number = "121",
pages = "51-59",
doi = "10.2298/ZMSPN1121051B",
url = "conv_58"
}
Bočarov-Stančić, A., Stanković, S., Lević, J., Salma, N. M., Pantić, V. R.,& Barnić, S. S.. (2011). In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(121), 51-59.
https://doi.org/10.2298/ZMSPN1121051B
conv_58
Bočarov-Stančić A, Stanković S, Lević J, Salma NM, Pantić VR, Barnić SS. In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate. in Zbornik Matice srpske za prirodne nauke. 2011;(121):51-59.
doi:10.2298/ZMSPN1121051B
conv_58 .
Bočarov-Stančić, Aleksandra, Stanković, Slavica, Lević, Jelena, Salma, Nataša M., Pantić, Vladimir R., Barnić, Saša S., "In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate" in Zbornik Matice srpske za prirodne nauke, no. 121 (2011):51-59,
https://doi.org/10.2298/ZMSPN1121051B .,
conv_58 .
2

Investigation of toxigenic potential of fungal species by the use of simple screening method

Bočarov-Stančić, Aleksandra; Lević, Jelena; Dimić, Gordana R.; Stanković, Slavica; Salma, Nataša M.

(Matica srpska, Novi Sad, 2009)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Dimić, Gordana R.
AU  - Stanković, Slavica
AU  - Salma, Nataša M.
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/281
AB  - Potential for the biosynthesis of aflatoxin B1 (AFLB1), ochratoxin A (OTA), diacetoxyscirpenol (DAS), T-2 toxin (T2), and zearalenone (ZON) was investigated in different fungal species belonging to the genera: Aspergillus, Fusarium and Penicillium. The majority of investigated isolates originated from cereal grains, crushed oil soybean seed and fodder mixtures. The simple screening method developed by Filtenborg et al. (1983) was applied with few modifications concerning the type of the medium and cultivation temperature. In order to optimise the biosynthetic conditions for different mycotoxins, the following control cultures, known as mycotin producers were used: OTA - A. ochraceus CBS 108.08, DAS - F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) and ZON - F. graminearum (GZ-LES). The fungi were cultivated on the standard medium (YESA - 2% yeast extract, 15% sucrose and 2% agar, pH 6.5), three modifications of the basic medium (YESAZn - the standard medium supplemented with 0.23 mg/l ZnSO4 x 5 H2O; PPSA - the medium in which yeast extract was replaced with peptone-1; PPSAZn - the medium in which yeast extract was replaced with peptone-1 and supplemented with 0.23 mg/l ZnSO4 x 5 H2O), and the potato-dextrose agar (PDA). The earlier biosynthesis of tested mycotoxins was recorded under the following cultivation conditions of fungal species: AFLB1 - after 14 days on PDA at 27±1°C, OTA - after 10 days on YESA and YESAZn at 27±1°C, DAS - after 10 days on PPSA and PPSAZn at 27±1°C, T2 - after 7 days on PPSAZn and PPSA at room temperature (20-24°C), and ZON - after 1 week on YESA and YESAZn at room temperature (21-24°C).
AB  - Potencijal za biosintezu aflatoksina B1 (AFLB1), ohratoksina A (OTA), diacetoksiscirpenola (DAS), T-2 toksina (T2) i zearalenona je ispitan kod izolata gljiva koje pripadaju rodovima Aspergillus, Fusarium i Penicillium. Izolati su uglavnom bili poreklom sa zrna žitarica i stočne hrane. Primenjena je jednostavna trijažna metoda F i l t e n b o r g-a i sar. (1983) kod koje su izvršene izvesne modifikacije u smislu tipa podloge i temperature kultivacije gljiva. U cilju optimizacije uslova za testiranje toksigenog profila odabranih gljivičnih izolata upotrebljene su kontrolne kulture za koje je prethodno dokazano da su proizvođači sledećih mikotoksina: OTA-a A. ochraceus CBS 108.08, DAS-a F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) i ZON-a F. graminearum (GZ-LES). Gljive su gajene na standardnoj podlozi (EKSA - 2% ekstrakta kvasca i 15% saharoze, pH 6,5), tri modifikacije osnovne podloge (EKSAZn-standardna podloga sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O; PPSA - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1; PPSAZn - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1 i kojoj je dodato 0,23 mg/l ZnSO4 x 5 H2O; pH 6,5) i krompir dekstroznoj podlozi (pH 6,5). Biosinteza ispitanih mikotoksina je najranije konstatovana pri sledećim uslovima gajenja gljiva: AFLB1 - posle 14 dana kultivacije na KDA i 27±1°C, OTA - posle 10 dana kultivacije na EKSA i EKSAZn i 27±1°C, DAS-a posle 10 dana kultivacije na PPSA i PPSAZn na 27±1°C, T2 - posle 7 dana na PPSA i PPSAZn i sobnoj temperaturi (20-24°C), i ZON-a posle nedelju dana na EKSA i EKSAZn i sobnoj temperaturi (21-24°C).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Investigation of toxigenic potential of fungal species by the use of simple screening method
T1  - Ispitivanje toksigenog potencijala gljiva primenom jednostavnog trijažnog metoda
IS  - 116
SP  - 25
EP  - 32
DO  - 10.2298/ZMSPN0916025B
UR  - conv_51
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Dimić, Gordana R. and Stanković, Slavica and Salma, Nataša M.",
year = "2009",
abstract = "Potential for the biosynthesis of aflatoxin B1 (AFLB1), ochratoxin A (OTA), diacetoxyscirpenol (DAS), T-2 toxin (T2), and zearalenone (ZON) was investigated in different fungal species belonging to the genera: Aspergillus, Fusarium and Penicillium. The majority of investigated isolates originated from cereal grains, crushed oil soybean seed and fodder mixtures. The simple screening method developed by Filtenborg et al. (1983) was applied with few modifications concerning the type of the medium and cultivation temperature. In order to optimise the biosynthetic conditions for different mycotoxins, the following control cultures, known as mycotin producers were used: OTA - A. ochraceus CBS 108.08, DAS - F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) and ZON - F. graminearum (GZ-LES). The fungi were cultivated on the standard medium (YESA - 2% yeast extract, 15% sucrose and 2% agar, pH 6.5), three modifications of the basic medium (YESAZn - the standard medium supplemented with 0.23 mg/l ZnSO4 x 5 H2O; PPSA - the medium in which yeast extract was replaced with peptone-1; PPSAZn - the medium in which yeast extract was replaced with peptone-1 and supplemented with 0.23 mg/l ZnSO4 x 5 H2O), and the potato-dextrose agar (PDA). The earlier biosynthesis of tested mycotoxins was recorded under the following cultivation conditions of fungal species: AFLB1 - after 14 days on PDA at 27±1°C, OTA - after 10 days on YESA and YESAZn at 27±1°C, DAS - after 10 days on PPSA and PPSAZn at 27±1°C, T2 - after 7 days on PPSAZn and PPSA at room temperature (20-24°C), and ZON - after 1 week on YESA and YESAZn at room temperature (21-24°C)., Potencijal za biosintezu aflatoksina B1 (AFLB1), ohratoksina A (OTA), diacetoksiscirpenola (DAS), T-2 toksina (T2) i zearalenona je ispitan kod izolata gljiva koje pripadaju rodovima Aspergillus, Fusarium i Penicillium. Izolati su uglavnom bili poreklom sa zrna žitarica i stočne hrane. Primenjena je jednostavna trijažna metoda F i l t e n b o r g-a i sar. (1983) kod koje su izvršene izvesne modifikacije u smislu tipa podloge i temperature kultivacije gljiva. U cilju optimizacije uslova za testiranje toksigenog profila odabranih gljivičnih izolata upotrebljene su kontrolne kulture za koje je prethodno dokazano da su proizvođači sledećih mikotoksina: OTA-a A. ochraceus CBS 108.08, DAS-a F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) i ZON-a F. graminearum (GZ-LES). Gljive su gajene na standardnoj podlozi (EKSA - 2% ekstrakta kvasca i 15% saharoze, pH 6,5), tri modifikacije osnovne podloge (EKSAZn-standardna podloga sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O; PPSA - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1; PPSAZn - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1 i kojoj je dodato 0,23 mg/l ZnSO4 x 5 H2O; pH 6,5) i krompir dekstroznoj podlozi (pH 6,5). Biosinteza ispitanih mikotoksina je najranije konstatovana pri sledećim uslovima gajenja gljiva: AFLB1 - posle 14 dana kultivacije na KDA i 27±1°C, OTA - posle 10 dana kultivacije na EKSA i EKSAZn i 27±1°C, DAS-a posle 10 dana kultivacije na PPSA i PPSAZn na 27±1°C, T2 - posle 7 dana na PPSA i PPSAZn i sobnoj temperaturi (20-24°C), i ZON-a posle nedelju dana na EKSA i EKSAZn i sobnoj temperaturi (21-24°C).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Investigation of toxigenic potential of fungal species by the use of simple screening method, Ispitivanje toksigenog potencijala gljiva primenom jednostavnog trijažnog metoda",
number = "116",
pages = "25-32",
doi = "10.2298/ZMSPN0916025B",
url = "conv_51"
}
Bočarov-Stančić, A., Lević, J., Dimić, G. R., Stanković, S.,& Salma, N. M.. (2009). Investigation of toxigenic potential of fungal species by the use of simple screening method. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(116), 25-32.
https://doi.org/10.2298/ZMSPN0916025B
conv_51
Bočarov-Stančić A, Lević J, Dimić GR, Stanković S, Salma NM. Investigation of toxigenic potential of fungal species by the use of simple screening method. in Zbornik Matice srpske za prirodne nauke. 2009;(116):25-32.
doi:10.2298/ZMSPN0916025B
conv_51 .
Bočarov-Stančić, Aleksandra, Lević, Jelena, Dimić, Gordana R., Stanković, Slavica, Salma, Nataša M., "Investigation of toxigenic potential of fungal species by the use of simple screening method" in Zbornik Matice srpske za prirodne nauke, no. 116 (2009):25-32,
https://doi.org/10.2298/ZMSPN0916025B .,
conv_51 .
7

Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels

Bočarov-Stančić, Aleksandra; Lević, Jelena; Stanković, Slavica; Tančić, Sonja; Krnjaja, Vesna; Salma, Nataša M.

(Akademiai Kiado Zrt, Budapest, 2008)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Tančić, Sonja
AU  - Krnjaja, Vesna
AU  - Salma, Nataša M.
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/238
AB  - The toxigenic potential of F. langsethiae cultures isolated from Serbian wheat kernels harvested in 2005 was investigated. In vitro experiments were performed at room temperature (24-28 degrees C) with two different media: liquid GPYE and wet( sterilized wheat grain. All of the tested F. langsethiae isolates produced T-2 toxin (0.312 - 48.0 ppm) and DAS (0.3 12-12.0 ppm), but only one (MRIZP-1208) zearalenone.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Cereal Research Communications
T1  - Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels
VL  - 36
SP  - 345
EP  - 346
DO  - 10.1556/CRC.36.2008.Suppl.B.33
UR  - conv_708
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Stanković, Slavica and Tančić, Sonja and Krnjaja, Vesna and Salma, Nataša M.",
year = "2008",
abstract = "The toxigenic potential of F. langsethiae cultures isolated from Serbian wheat kernels harvested in 2005 was investigated. In vitro experiments were performed at room temperature (24-28 degrees C) with two different media: liquid GPYE and wet( sterilized wheat grain. All of the tested F. langsethiae isolates produced T-2 toxin (0.312 - 48.0 ppm) and DAS (0.3 12-12.0 ppm), but only one (MRIZP-1208) zearalenone.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Cereal Research Communications",
title = "Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels",
volume = "36",
pages = "345-346",
doi = "10.1556/CRC.36.2008.Suppl.B.33",
url = "conv_708"
}
Bočarov-Stančić, A., Lević, J., Stanković, S., Tančić, S., Krnjaja, V.,& Salma, N. M.. (2008). Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels. in Cereal Research Communications
Akademiai Kiado Zrt, Budapest., 36, 345-346.
https://doi.org/10.1556/CRC.36.2008.Suppl.B.33
conv_708
Bočarov-Stančić A, Lević J, Stanković S, Tančić S, Krnjaja V, Salma NM. Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels. in Cereal Research Communications. 2008;36:345-346.
doi:10.1556/CRC.36.2008.Suppl.B.33
conv_708 .
Bočarov-Stančić, Aleksandra, Lević, Jelena, Stanković, Slavica, Tančić, Sonja, Krnjaja, Vesna, Salma, Nataša M., "Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels" in Cereal Research Communications, 36 (2008):345-346,
https://doi.org/10.1556/CRC.36.2008.Suppl.B.33 .,
conv_708 .
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