Nikolić, Ana

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orcid::0000-0002-9883-7479
  • Nikolić, Ana (53)
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Author's Bibliography

ZP5023

Božinović, Sofija; Vančetović, Jelena; Grčić, Nikola; Nikolić, Ana; Đorđević Melnik, Olivera

(2021)

TY  - PAT
AU  - Božinović, Sofija
AU  - Vančetović, Jelena
AU  - Grčić, Nikola
AU  - Nikolić, Ana
AU  - Đorđević Melnik, Olivera
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1102
T2  - Rešenje:320-44-02159/2/2020-11
T1  - ZP5023
ER  - 
@misc{
author = "Božinović, Sofija and Vančetović, Jelena and Grčić, Nikola and Nikolić, Ana and Đorđević Melnik, Olivera",
year = "2021",
journal = "Rešenje:320-44-02159/2/2020-11",
title = "ZP5023"
}
Božinović, S., Vančetović, J., Grčić, N., Nikolić, A.,& Đorđević Melnik, O.. (2021). ZP5023. in Rešenje:320-44-02159/2/2020-11.
Božinović S, Vančetović J, Grčić N, Nikolić A, Đorđević Melnik O. ZP5023. in Rešenje:320-44-02159/2/2020-11. 2021;..
Božinović, Sofija, Vančetović, Jelena, Grčić, Nikola, Nikolić, Ana, Đorđević Melnik, Olivera, "ZP5023" in Rešenje:320-44-02159/2/2020-11 (2021).

Molecular characterization of popcorn inbreds using 25k SNP array

Mladenović Drinić, Snežana; Srdić, Jelena; Anđelković, Violeta; Grčić, Nikola; Kravić, Natalija; Filipović, Milomir; Nikolić, Ana

(Belgrade : Maize Research Institute, Zemun Polje, 2022)

TY  - CONF
AU  - Mladenović Drinić, Snežana
AU  - Srdić, Jelena
AU  - Anđelković, Violeta
AU  - Grčić, Nikola
AU  - Kravić, Natalija
AU  - Filipović, Milomir
AU  - Nikolić, Ana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/958
AB  - Popcorn is a type of specialty maize and plays important role in the history and the spread of
this species. This type of maize historicaly received little atention in genetic diversity studies
while the fact is that information on genetic variability and population structure is of crucial
importance for improvement of breeding programs. The genetic variation of popcorn maize
was mainly analysed using SSR markers, but there are not many studies applying high-density
SNP markers with lower genotyping errors. The aim of this study was the assesment of genetic
diversity and population structure of popcorn germplasm which represents the genetic
resources currently used in breeding programs in Maize Research Institute „Zemun Polje“.
Molecular characterization of 44 popcorn genotypes was done using 25k SNP Illumina Infinum
Array for maize. After quality control filtering, 12335 SNPs were selected for downstream
analyses. Genetic distance detected ranged from 0.001 to 0.51 with an average of 0.33. The
majority of genetic distances (70.6%) fell between 0.30 and 0.51 revealing significant genetic
variability in a set of maize genotypes under study. SNP markes were moderatly informative
with an average PIC value of 0.26. Also, for more than 50% of SNPs, PIC value was greater
than 0.30. The average observed heterozygosity was 0.04 ranging from 0 to 0.11. The
clustering, principal component and model-based STRUCTURE analyses showed similar
grouping of popcorn lines, generally in accordance with their genetic background. The results
of this research gave valuable information for initiation of future breeding strategies.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Molecular characterization of popcorn inbreds using 25k SNP array
ER  - 
@conference{
author = "Mladenović Drinić, Snežana and Srdić, Jelena and Anđelković, Violeta and Grčić, Nikola and Kravić, Natalija and Filipović, Milomir and Nikolić, Ana",
year = "2022",
abstract = "Popcorn is a type of specialty maize and plays important role in the history and the spread of
this species. This type of maize historicaly received little atention in genetic diversity studies
while the fact is that information on genetic variability and population structure is of crucial
importance for improvement of breeding programs. The genetic variation of popcorn maize
was mainly analysed using SSR markers, but there are not many studies applying high-density
SNP markers with lower genotyping errors. The aim of this study was the assesment of genetic
diversity and population structure of popcorn germplasm which represents the genetic
resources currently used in breeding programs in Maize Research Institute „Zemun Polje“.
Molecular characterization of 44 popcorn genotypes was done using 25k SNP Illumina Infinum
Array for maize. After quality control filtering, 12335 SNPs were selected for downstream
analyses. Genetic distance detected ranged from 0.001 to 0.51 with an average of 0.33. The
majority of genetic distances (70.6%) fell between 0.30 and 0.51 revealing significant genetic
variability in a set of maize genotypes under study. SNP markes were moderatly informative
with an average PIC value of 0.26. Also, for more than 50% of SNPs, PIC value was greater
than 0.30. The average observed heterozygosity was 0.04 ranging from 0 to 0.11. The
clustering, principal component and model-based STRUCTURE analyses showed similar
grouping of popcorn lines, generally in accordance with their genetic background. The results
of this research gave valuable information for initiation of future breeding strategies.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Molecular characterization of popcorn inbreds using 25k SNP array"
}
Mladenović Drinić, S., Srdić, J., Anđelković, V., Grčić, N., Kravić, N., Filipović, M.,& Nikolić, A.. (2022). Molecular characterization of popcorn inbreds using 25k SNP array. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje..
Mladenović Drinić S, Srdić J, Anđelković V, Grčić N, Kravić N, Filipović M, Nikolić A. Molecular characterization of popcorn inbreds using 25k SNP array. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;..
Mladenović Drinić, Snežana, Srdić, Jelena, Anđelković, Violeta, Grčić, Nikola, Kravić, Natalija, Filipović, Milomir, Nikolić, Ana, "Molecular characterization of popcorn inbreds using 25k SNP array" in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022).

Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia

Nikolić, Milica; Savić, Iva; Nikolić, Ana; Jauković, Marko; Kandić, Vesna; Stevanović, Milan; Stanković, Slavica

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Nikolić, Ana
AU  - Jauković, Marko
AU  - Kandić, Vesna
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/836
AB  - In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Toxins
T1  - Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia
VL  - 13
SP  - 847
DO  - 10.3390/toxins13120847
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Nikolić, Ana and Jauković, Marko and Kandić, Vesna and Stevanović, Milan and Stanković, Slavica",
year = "2021",
abstract = "In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Toxins",
title = "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia",
volume = "13",
pages = "847",
doi = "10.3390/toxins13120847"
}
Nikolić, M., Savić, I., Nikolić, A., Jauković, M., Kandić, V., Stevanović, M.,& Stanković, S.. (2021). Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 13, 847.
https://doi.org/10.3390/toxins13120847
Nikolić M, Savić I, Nikolić A, Jauković M, Kandić V, Stevanović M, Stanković S. Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins. 2021;13:847.
doi:10.3390/toxins13120847 .
Nikolić, Milica, Savić, Iva, Nikolić, Ana, Jauković, Marko, Kandić, Vesna, Stevanović, Milan, Stanković, Slavica, "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia" in Toxins, 13 (2021):847,
https://doi.org/10.3390/toxins13120847 . .
1

ZP6091

Grčić, Nikola; Filipović, Milomir; Nikolić, Ana; Mladenović, Marko

(2021)

TY  - PAT
AU  - Grčić, Nikola
AU  - Filipović, Milomir
AU  - Nikolić, Ana
AU  - Mladenović, Marko
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1083
T2  - Rešenje:320-04-02261/2/2019-11
T1  - ZP6091
ER  - 
@misc{
author = "Grčić, Nikola and Filipović, Milomir and Nikolić, Ana and Mladenović, Marko",
year = "2021",
journal = "Rešenje:320-04-02261/2/2019-11",
title = "ZP6091"
}
Grčić, N., Filipović, M., Nikolić, A.,& Mladenović, M.. (2021). ZP6091. in Rešenje:320-04-02261/2/2019-11.
Grčić N, Filipović M, Nikolić A, Mladenović M. ZP6091. in Rešenje:320-04-02261/2/2019-11. 2021;..
Grčić, Nikola, Filipović, Milomir, Nikolić, Ana, Mladenović, Marko, "ZP6091" in Rešenje:320-04-02261/2/2019-11 (2021).

Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions

Božić, Manja; Stanojević, Aleksandra; Marković, Ksenija; Ignjatović-Micić, Dragana; Nikolić, Dragana; Milivojević, Marija; Nikolić, Ana

(Belgrade : Serbian Genetics Society, 2021)

TY  - JOUR
AU  - Božić, Manja
AU  - Stanojević, Aleksandra
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Dragana
AU  - Milivojević, Marija
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/832
AB  - Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance.
AB  - Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions
VL  - 53
IS  - 3
SP  - 1147
EP  - 1165
DO  - 10.2298/GENSR2103147B
ER  - 
@article{
author = "Božić, Manja and Stanojević, Aleksandra and Marković, Ksenija and Ignjatović-Micić, Dragana and Nikolić, Dragana and Milivojević, Marija and Nikolić, Ana",
year = "2021",
abstract = "Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance., Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions",
volume = "53",
number = "3",
pages = "1147-1165",
doi = "10.2298/GENSR2103147B"
}
Božić, M., Stanojević, A., Marković, K., Ignjatović-Micić, D., Nikolić, D., Milivojević, M.,& Nikolić, A.. (2021). Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika
Belgrade : Serbian Genetics Society., 53(3), 1147-1165.
https://doi.org/10.2298/GENSR2103147B
Božić M, Stanojević A, Marković K, Ignjatović-Micić D, Nikolić D, Milivojević M, Nikolić A. Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika. 2021;53(3):1147-1165.
doi:10.2298/GENSR2103147B .
Božić, Manja, Stanojević, Aleksandra, Marković, Ksenija, Ignjatović-Micić, Dragana, Nikolić, Dragana, Milivojević, Marija, Nikolić, Ana, "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions" in Genetika, 53, no. 3 (2021):1147-1165,
https://doi.org/10.2298/GENSR2103147B . .

Morphological and physiological response of maize seedlings to chilling stress

Nikolić, Ana; Kravić, Natalija; Ristić, Danijela; Anđelković, Violeta; Marković, Ksenija; Vančetović, Jelena; Ignjatović-Micić, Dragana

(Beograd : Društavo genetičara Srbije, 2020)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kravić, Natalija
AU  - Ristić, Danijela
AU  - Anđelković, Violeta
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/814
AB  - Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing.
AB  - Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve
PB  - Beograd : Društavo genetičara Srbije
T2  - Genetika
T1  - Morphological and physiological response of maize seedlings to chilling stress
VL  - 52
IS  - 2
SP  - 689
EP  - 698
DO  - 10.2298/GENSR2002689N
ER  - 
@article{
author = "Nikolić, Ana and Kravić, Natalija and Ristić, Danijela and Anđelković, Violeta and Marković, Ksenija and Vančetović, Jelena and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing., Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve",
publisher = "Beograd : Društavo genetičara Srbije",
journal = "Genetika",
title = "Morphological and physiological response of maize seedlings to chilling stress",
volume = "52",
number = "2",
pages = "689-698",
doi = "10.2298/GENSR2002689N"
}
Nikolić, A., Kravić, N., Ristić, D., Anđelković, V., Marković, K., Vančetović, J.,& Ignjatović-Micić, D.. (2020). Morphological and physiological response of maize seedlings to chilling stress. in Genetika
Beograd : Društavo genetičara Srbije., 52(2), 689-698.
https://doi.org/10.2298/GENSR2002689N
Nikolić A, Kravić N, Ristić D, Anđelković V, Marković K, Vančetović J, Ignjatović-Micić D. Morphological and physiological response of maize seedlings to chilling stress. in Genetika. 2020;52(2):689-698.
doi:10.2298/GENSR2002689N .
Nikolić, Ana, Kravić, Natalija, Ristić, Danijela, Anđelković, Violeta, Marković, Ksenija, Vančetović, Jelena, Ignjatović-Micić, Dragana, "Morphological and physiological response of maize seedlings to chilling stress" in Genetika, 52, no. 2 (2020):689-698,
https://doi.org/10.2298/GENSR2002689N . .
3
2

Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions

Vančetović, Jelena; Kostadinović, Marija; Božinović, Sofija; Nikolić, Ana; Vukadinović, Jelena; Marković, Ksenija; Ignjatović-Micić, Dragana

(Beograd : Društvo genetičara Srbije, 2020)

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Vukadinović, Jelena
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/791
AB  - Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins.
AB  - Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.
PB  - Beograd : Društvo genetičara Srbije
T2  - Genetika
T1  - Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions
T1  - Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena
VL  - 52
IS  - 1
SP  - 273
EP  - 289
DO  - 10.2298/GENSR2001273V
ER  - 
@article{
author = "Vančetović, Jelena and Kostadinović, Marija and Božinović, Sofija and Nikolić, Ana and Vukadinović, Jelena and Marković, Ksenija and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins., Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "Genetika",
title = "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions, Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena",
volume = "52",
number = "1",
pages = "273-289",
doi = "10.2298/GENSR2001273V"
}
Vančetović, J., Kostadinović, M., Božinović, S., Nikolić, A., Vukadinović, J., Marković, K.,& Ignjatović-Micić, D.. (2020). Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika
Beograd : Društvo genetičara Srbije., 52(1), 273-289.
https://doi.org/10.2298/GENSR2001273V
Vančetović J, Kostadinović M, Božinović S, Nikolić A, Vukadinović J, Marković K, Ignjatović-Micić D. Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika. 2020;52(1):273-289.
doi:10.2298/GENSR2001273V .
Vančetović, Jelena, Kostadinović, Marija, Božinović, Sofija, Nikolić, Ana, Vukadinović, Jelena, Marković, Ksenija, Ignjatović-Micić, Dragana, "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions" in Genetika, 52, no. 1 (2020):273-289,
https://doi.org/10.2298/GENSR2001273V . .

Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress

Dodig, Dejan; Božinović, Sofija; Nikolić, Ana; Zorić, Miroslav; Vančetović, Jelena; Ignjatović-Micić, Dragana; Delić, Nenad; Weigelt-Fischer, Kathleen; Junker, Astrid; Altmann, Thomas

(Lausanne : Frontiers Media SA, 2019)

TY  - JOUR
AU  - Dodig, Dejan
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Zorić, Miroslav
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Delić, Nenad
AU  - Weigelt-Fischer, Kathleen
AU  - Junker, Astrid
AU  - Altmann, Thomas
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/812
AB  - Phenotypic measurements under controlled cultivation conditions are essential to gain
a mechanistic understanding of plant responses to environmental impacts and thus
for knowledge-based improvement of their performance under natural field conditions.
Twenty maize inbred lines (ILs) were phenotyped in response to two levels of water and
nitrogen supply (control and stress) and combined nitrogen and water deficit. Over a
course of 5 weeks (from about 4-leaf stage to the beginning of the reproductive stage),
maize phenology and growth were monitored by using a high-throughput phenotyping
platform for daily acquisition of images in different spectral ranges. The focus of the
present study is on the measurements taken at the time of maximum water stress (for
traits that reflect plant physiological properties) and at the end of the experiment (for
traits that reflect plant architectural and biomass-related traits). Twenty-five phenotypic
traits extracted from the digital image data that support biological interpretation of
plant growth were selected for their predictive value for mid-season shoot biomass
accumulation. Measured fresh and dry weights after harvest were used to calculate
various indices (water-use efficiency, physiological nitrogen-use efficiency, specific plant
weight) and to establish correlations with image-derived phenotypic features. Also, score
indices based on dry weight were used to identify contrasting ILs in terms of productivity
and tolerance to stress, and their means for image-derived and manually measured traits
were compared. Color-related traits appear to be indicative of plant performance and
photosystem II operating efficiency might be an importance physiological parameter
of biomass accumulation, particularly under severe stress conditions. Also, genotypes
showing greater leaf area may be better adapted to abiotic stress conditions.
PB  - Lausanne : Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress
VL  - 10
SP  - 814
DO  - 10.3389/fpls.2019.00814
ER  - 
@article{
author = "Dodig, Dejan and Božinović, Sofija and Nikolić, Ana and Zorić, Miroslav and Vančetović, Jelena and Ignjatović-Micić, Dragana and Delić, Nenad and Weigelt-Fischer, Kathleen and Junker, Astrid and Altmann, Thomas",
year = "2019",
abstract = "Phenotypic measurements under controlled cultivation conditions are essential to gain
a mechanistic understanding of plant responses to environmental impacts and thus
for knowledge-based improvement of their performance under natural field conditions.
Twenty maize inbred lines (ILs) were phenotyped in response to two levels of water and
nitrogen supply (control and stress) and combined nitrogen and water deficit. Over a
course of 5 weeks (from about 4-leaf stage to the beginning of the reproductive stage),
maize phenology and growth were monitored by using a high-throughput phenotyping
platform for daily acquisition of images in different spectral ranges. The focus of the
present study is on the measurements taken at the time of maximum water stress (for
traits that reflect plant physiological properties) and at the end of the experiment (for
traits that reflect plant architectural and biomass-related traits). Twenty-five phenotypic
traits extracted from the digital image data that support biological interpretation of
plant growth were selected for their predictive value for mid-season shoot biomass
accumulation. Measured fresh and dry weights after harvest were used to calculate
various indices (water-use efficiency, physiological nitrogen-use efficiency, specific plant
weight) and to establish correlations with image-derived phenotypic features. Also, score
indices based on dry weight were used to identify contrasting ILs in terms of productivity
and tolerance to stress, and their means for image-derived and manually measured traits
were compared. Color-related traits appear to be indicative of plant performance and
photosystem II operating efficiency might be an importance physiological parameter
of biomass accumulation, particularly under severe stress conditions. Also, genotypes
showing greater leaf area may be better adapted to abiotic stress conditions.",
publisher = "Lausanne : Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress",
volume = "10",
pages = "814",
doi = "10.3389/fpls.2019.00814"
}
Dodig, D., Božinović, S., Nikolić, A., Zorić, M., Vančetović, J., Ignjatović-Micić, D., Delić, N., Weigelt-Fischer, K., Junker, A.,& Altmann, T.. (2019). Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress. in Frontiers in Plant Science
Lausanne : Frontiers Media SA., 10, 814.
https://doi.org/10.3389/fpls.2019.00814
Dodig D, Božinović S, Nikolić A, Zorić M, Vančetović J, Ignjatović-Micić D, Delić N, Weigelt-Fischer K, Junker A, Altmann T. Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress. in Frontiers in Plant Science. 2019;10:814.
doi:10.3389/fpls.2019.00814 .
Dodig, Dejan, Božinović, Sofija, Nikolić, Ana, Zorić, Miroslav, Vančetović, Jelena, Ignjatović-Micić, Dragana, Delić, Nenad, Weigelt-Fischer, Kathleen, Junker, Astrid, Altmann, Thomas, "Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress" in Frontiers in Plant Science, 10 (2019):814,
https://doi.org/10.3389/fpls.2019.00814 . .
7
10
5

Marker assisted backcross breeding in Maize Research Institute Zemun Polje

Kostadinović, Marija; Nikolić, Ana; Ristić, Danijela; Božinović, Sofija; Đorđević-Melnik, Olivera; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2019)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/743
AB  - Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije.
AB  - The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Marker assisted backcross breeding in Maize Research Institute Zemun Polje
T1  - Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje
VL  - 25
IS  - 1
SP  - 41
EP  - 47
DO  - 10.5937/SelSem1901041K
UR  - conv_2003
ER  - 
@article{
author = "Kostadinović, Marija and Nikolić, Ana and Ristić, Danijela and Božinović, Sofija and Đorđević-Melnik, Olivera and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije., The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Marker assisted backcross breeding in Maize Research Institute Zemun Polje, Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje",
volume = "25",
number = "1",
pages = "41-47",
doi = "10.5937/SelSem1901041K",
url = "conv_2003"
}
Kostadinović, M., Nikolić, A., Ristić, D., Božinović, S., Đorđević-Melnik, O., Ignjatović-Micić, D.,& Vančetović, J.. (2019). Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 25(1), 41-47.
https://doi.org/10.5937/SelSem1901041K
conv_2003
Kostadinović M, Nikolić A, Ristić D, Božinović S, Đorđević-Melnik O, Ignjatović-Micić D, Vančetović J. Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo. 2019;25(1):41-47.
doi:10.5937/SelSem1901041K
conv_2003 .
Kostadinović, Marija, Nikolić, Ana, Ristić, Danijela, Božinović, Sofija, Đorđević-Melnik, Olivera, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted backcross breeding in Maize Research Institute Zemun Polje" in Selekcija i semenarstvo, 25, no. 1 (2019):41-47,
https://doi.org/10.5937/SelSem1901041K .,
conv_2003 .
3

Grain quality within ex situ and in situ conserved traditional white maize landraces

Babić, Vojka; Kravić, Natalija; Srdić, Jelena; Popović, Aleksandar; Nikolić, Ana; Zivić, Jovana; Miritescu, Mihai

(Natl Agricultural Research & Development Inst, Fundulea, 2018)

TY  - JOUR
AU  - Babić, Vojka
AU  - Kravić, Natalija
AU  - Srdić, Jelena
AU  - Popović, Aleksandar
AU  - Nikolić, Ana
AU  - Zivić, Jovana
AU  - Miritescu, Mihai
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/731
AB  - White maize had an important role in human nutrition throughout the Western Balkan region. A great number of farmers (approximately 86% in Serbia) still grow traditional white Open Pollinated Varieties (OPVs) for human diet. Out of 2217 maize landraces stored within Maize Research Institute "Zemun Polje" (MRIZP) gene bank, more than 700 are white kernel accessions. Eighteen white maize landraces, collected in the Western Balkan region in different periods, were selected for the present study. By the evaluation of agromorphological performances and the basic chemical composition of grain, the objective was to determine the importance of landraces conserved under in situ and ex situ conditions, to be used as the initial material for breeding. The idea was to determine whether the varieties conserved under ex situ conditions (collected from the same or different regions) distinguish from varieties permanently grown in the region of western Serbia. It was found that varieties permanently grown in the western Serbia were more similar to varieties of the ex situ collection from eastern Bosnia and Herzegovina than to varieties collected in western Serbia 50 years ago. The comparison between OPVs and modern white maize hybrids for the basic chemical composition of grain showed the significant potential of OPVs for nutritive grain quality improvement. Increased organic production creates new possibilities for more intensive incorporation of OPVs into this agricultural system. New opportunities for the cooperation and share of responsibilities among breeders, farmers and genetic resources managers are initiated, to be used for in situ maize genetic resources conservation improvement. More detailed characterisation and evaluation of the white maize landraces will offer an explanation of what has motivated our farmers to maintain the OPVs production through centuries, despite the availability of modern hybrids.
PB  - Natl Agricultural Research & Development Inst, Fundulea
T2  - Romanian Agricultural Research
T1  - Grain quality within ex situ and in situ conserved traditional white maize landraces
VL  - 35
SP  - 129
EP  - 140
UR  - conv_988
ER  - 
@article{
author = "Babić, Vojka and Kravić, Natalija and Srdić, Jelena and Popović, Aleksandar and Nikolić, Ana and Zivić, Jovana and Miritescu, Mihai",
year = "2018",
abstract = "White maize had an important role in human nutrition throughout the Western Balkan region. A great number of farmers (approximately 86% in Serbia) still grow traditional white Open Pollinated Varieties (OPVs) for human diet. Out of 2217 maize landraces stored within Maize Research Institute "Zemun Polje" (MRIZP) gene bank, more than 700 are white kernel accessions. Eighteen white maize landraces, collected in the Western Balkan region in different periods, were selected for the present study. By the evaluation of agromorphological performances and the basic chemical composition of grain, the objective was to determine the importance of landraces conserved under in situ and ex situ conditions, to be used as the initial material for breeding. The idea was to determine whether the varieties conserved under ex situ conditions (collected from the same or different regions) distinguish from varieties permanently grown in the region of western Serbia. It was found that varieties permanently grown in the western Serbia were more similar to varieties of the ex situ collection from eastern Bosnia and Herzegovina than to varieties collected in western Serbia 50 years ago. The comparison between OPVs and modern white maize hybrids for the basic chemical composition of grain showed the significant potential of OPVs for nutritive grain quality improvement. Increased organic production creates new possibilities for more intensive incorporation of OPVs into this agricultural system. New opportunities for the cooperation and share of responsibilities among breeders, farmers and genetic resources managers are initiated, to be used for in situ maize genetic resources conservation improvement. More detailed characterisation and evaluation of the white maize landraces will offer an explanation of what has motivated our farmers to maintain the OPVs production through centuries, despite the availability of modern hybrids.",
publisher = "Natl Agricultural Research & Development Inst, Fundulea",
journal = "Romanian Agricultural Research",
title = "Grain quality within ex situ and in situ conserved traditional white maize landraces",
volume = "35",
pages = "129-140",
url = "conv_988"
}
Babić, V., Kravić, N., Srdić, J., Popović, A., Nikolić, A., Zivić, J.,& Miritescu, M.. (2018). Grain quality within ex situ and in situ conserved traditional white maize landraces. in Romanian Agricultural Research
Natl Agricultural Research & Development Inst, Fundulea., 35, 129-140.
conv_988
Babić V, Kravić N, Srdić J, Popović A, Nikolić A, Zivić J, Miritescu M. Grain quality within ex situ and in situ conserved traditional white maize landraces. in Romanian Agricultural Research. 2018;35:129-140.
conv_988 .
Babić, Vojka, Kravić, Natalija, Srdić, Jelena, Popović, Aleksandar, Nikolić, Ana, Zivić, Jovana, Miritescu, Mihai, "Grain quality within ex situ and in situ conserved traditional white maize landraces" in Romanian Agricultural Research, 35 (2018):129-140,
conv_988 .
2
2

Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers

Anđelković, Violeta; Nikolić, Ana; Kovačević, Dragan; Mladenović Drinić, Snežana; Kravić, Natalija; Babić, Vojka; Srebrić, Mirjana; Jankulovska, Mirjana; Ivanovska, Sonja; Bosev, Dane

(Inst Investigaciones Agropecuarias, Chillan, 2018)

TY  - JOUR
AU  - Anđelković, Violeta
AU  - Nikolić, Ana
AU  - Kovačević, Dragan
AU  - Mladenović Drinić, Snežana
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Srebrić, Mirjana
AU  - Jankulovska, Mirjana
AU  - Ivanovska, Sonja
AU  - Bosev, Dane
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/713
AB  - In the second half of 20th century the awareness of importance of landraces for the future, led to organized collecting missions for numerous plant species. A total of 2217 maize (Zea mays L.) landraces, collected in the former Yugoslavia, are stored at Maize Research Institute (MRIZP) gene bank. During 2014, new collecting missions were organized in the eastern and western parts of Macedonia. According to collecting site and kernel type, 14 samples from the Faculty of Agricultural Sciences and Food, R. Macedonia were chosen for the comparison and identification of possible duplicates, through coupling with the 16 MRIZP gene bank accessions from the same area and kernel characteristics. Phenotypic characterization was done for 21 traits according to International Board for Plant Genetic Resources descriptors for maize. The Principal Component Analysis (PCA) identifies five PCs with Eigenvalue > 1, explaining 80% of the total phenotypic variation. The most discriminative traits with the strongest positive associations were tasseling and silking dates, plant height, leaf length and ear length. Compared to the ex-situ populations, the number of alleles and the number of specific alleles, showed a significant decrease in the in situ populations. Twelve unique alleles were detected in samples from MRIZP gene bank, and only four were found in new Macedonian samples. Cluster analysis of morphological and molecular markers distinguished groups of maize accessions with distinctive morphological traits and genetic profiles that will be useful for conservation, and management of gene bank collection, as well as for possible utilization in breeding.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers
VL  - 78
IS  - 1
SP  - 30
EP  - 38
DO  - 10.4067/S0718-58392018000100030
UR  - conv_976
ER  - 
@article{
author = "Anđelković, Violeta and Nikolić, Ana and Kovačević, Dragan and Mladenović Drinić, Snežana and Kravić, Natalija and Babić, Vojka and Srebrić, Mirjana and Jankulovska, Mirjana and Ivanovska, Sonja and Bosev, Dane",
year = "2018",
abstract = "In the second half of 20th century the awareness of importance of landraces for the future, led to organized collecting missions for numerous plant species. A total of 2217 maize (Zea mays L.) landraces, collected in the former Yugoslavia, are stored at Maize Research Institute (MRIZP) gene bank. During 2014, new collecting missions were organized in the eastern and western parts of Macedonia. According to collecting site and kernel type, 14 samples from the Faculty of Agricultural Sciences and Food, R. Macedonia were chosen for the comparison and identification of possible duplicates, through coupling with the 16 MRIZP gene bank accessions from the same area and kernel characteristics. Phenotypic characterization was done for 21 traits according to International Board for Plant Genetic Resources descriptors for maize. The Principal Component Analysis (PCA) identifies five PCs with Eigenvalue > 1, explaining 80% of the total phenotypic variation. The most discriminative traits with the strongest positive associations were tasseling and silking dates, plant height, leaf length and ear length. Compared to the ex-situ populations, the number of alleles and the number of specific alleles, showed a significant decrease in the in situ populations. Twelve unique alleles were detected in samples from MRIZP gene bank, and only four were found in new Macedonian samples. Cluster analysis of morphological and molecular markers distinguished groups of maize accessions with distinctive morphological traits and genetic profiles that will be useful for conservation, and management of gene bank collection, as well as for possible utilization in breeding.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers",
volume = "78",
number = "1",
pages = "30-38",
doi = "10.4067/S0718-58392018000100030",
url = "conv_976"
}
Anđelković, V., Nikolić, A., Kovačević, D., Mladenović Drinić, S., Kravić, N., Babić, V., Srebrić, M., Jankulovska, M., Ivanovska, S.,& Bosev, D.. (2018). Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 78(1), 30-38.
https://doi.org/10.4067/S0718-58392018000100030
conv_976
Anđelković V, Nikolić A, Kovačević D, Mladenović Drinić S, Kravić N, Babić V, Srebrić M, Jankulovska M, Ivanovska S, Bosev D. Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers. in Chilean Journal of Agricultural Research. 2018;78(1):30-38.
doi:10.4067/S0718-58392018000100030
conv_976 .
Anđelković, Violeta, Nikolić, Ana, Kovačević, Dragan, Mladenović Drinić, Snežana, Kravić, Natalija, Babić, Vojka, Srebrić, Mirjana, Jankulovska, Mirjana, Ivanovska, Sonja, Bosev, Dane, "Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers" in Chilean Journal of Agricultural Research, 78, no. 1 (2018):30-38,
https://doi.org/10.4067/S0718-58392018000100030 .,
conv_976 .
8
4
6

Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites

Nikolić, Biljana; Kovačević, Dragan; Mladenović Drinić, Snežana; Nikolić, Ana; Mitić, Zorica S.; Bojović, Srđan; Marin, Petar D.

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Kovačević, Dragan
AU  - Mladenović Drinić, Snežana
AU  - Nikolić, Ana
AU  - Mitić, Zorica S.
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/719
AB  - Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites
VL  - 50
IS  - 1
SP  - 69
EP  - 84
DO  - 10.2298/GENSR1801069N
UR  - conv_978
ER  - 
@article{
author = "Nikolić, Biljana and Kovačević, Dragan and Mladenović Drinić, Snežana and Nikolić, Ana and Mitić, Zorica S. and Bojović, Srđan and Marin, Petar D.",
year = "2018",
abstract = "Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites",
volume = "50",
number = "1",
pages = "69-84",
doi = "10.2298/GENSR1801069N",
url = "conv_978"
}
Nikolić, B., Kovačević, D., Mladenović Drinić, S., Nikolić, A., Mitić, Z. S., Bojović, S.,& Marin, P. D.. (2018). Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites. in Genetika
Društvo genetičara Srbije, Beograd., 50(1), 69-84.
https://doi.org/10.2298/GENSR1801069N
conv_978
Nikolić B, Kovačević D, Mladenović Drinić S, Nikolić A, Mitić ZS, Bojović S, Marin PD. Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites. in Genetika. 2018;50(1):69-84.
doi:10.2298/GENSR1801069N
conv_978 .
Nikolić, Biljana, Kovačević, Dragan, Mladenović Drinić, Snežana, Nikolić, Ana, Mitić, Zorica S., Bojović, Srđan, Marin, Petar D., "Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites" in Genetika, 50, no. 1 (2018):69-84,
https://doi.org/10.2298/GENSR1801069N .,
conv_978 .
4
3
5

Application of multivariate analysis for genotype evaluation in soybean

Perić, Vesna; Srebrić, Mirjana; Nikolić, Ana; Mladenović Drinić, Snežana

(2018)

TY  - CHAP
AU  - Perić, Vesna
AU  - Srebrić, Mirjana
AU  - Nikolić, Ana
AU  - Mladenović Drinić, Snežana
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/701
AB  - Multivariate analysis is a frequently used approach in breeding studies. Applied to multiple trait data, Principal Component Analysis (PCA) gives an opportunity to graphically display the relationships between important agronomic traits, evaluate the varieties on the basis of multiple traits, identify valuable breeding sources and recommend possible selection strategies. This study was conducted to evaluate agronomic performance of 31 early varieties (maturity group 0) from the soybean collection of the Maize Research Institute "Zemun Polje" (Belgrade, Serbia). PCA analysis was applied to identify the best performing genotypes considering multiple traits (seed yield, major yield components and parameters of technological quality of grain), and to determine the level of trait interdependence. Genotypes were tested in a randomized complete block design with three replications during two years (2011 and 2012) at two locations in Serbia (Zemun Polje and Pančevo). An analysis of variance (mixed model) exhibited significant effects of genotype (G), environment (E), and genotype × environment (G × E) for most of the traits. According to PCA biplot, seed yield per plant was in positive correlation with number of seeds and pods per plant, indicating that those yield components could be effective selection criteria in breeding for seed yield. Comparison of early-maturing genotypes based on multiple traits revealed two varieties with above-average seed yield per plant and four varieties with high oil and above average protein content, which could be used as potential parents in breeding for important agronomic traits in future breeding programs.
T2  - Breeding Grasses and Protein Crops in the Era of Genomics
T1  - Application of multivariate analysis for genotype evaluation in soybean
SP  - 215
EP  - 219
DO  - 10.1007/978-3-319-89578-9_39
UR  - conv_1010
ER  - 
@inbook{
author = "Perić, Vesna and Srebrić, Mirjana and Nikolić, Ana and Mladenović Drinić, Snežana",
year = "2018",
abstract = "Multivariate analysis is a frequently used approach in breeding studies. Applied to multiple trait data, Principal Component Analysis (PCA) gives an opportunity to graphically display the relationships between important agronomic traits, evaluate the varieties on the basis of multiple traits, identify valuable breeding sources and recommend possible selection strategies. This study was conducted to evaluate agronomic performance of 31 early varieties (maturity group 0) from the soybean collection of the Maize Research Institute "Zemun Polje" (Belgrade, Serbia). PCA analysis was applied to identify the best performing genotypes considering multiple traits (seed yield, major yield components and parameters of technological quality of grain), and to determine the level of trait interdependence. Genotypes were tested in a randomized complete block design with three replications during two years (2011 and 2012) at two locations in Serbia (Zemun Polje and Pančevo). An analysis of variance (mixed model) exhibited significant effects of genotype (G), environment (E), and genotype × environment (G × E) for most of the traits. According to PCA biplot, seed yield per plant was in positive correlation with number of seeds and pods per plant, indicating that those yield components could be effective selection criteria in breeding for seed yield. Comparison of early-maturing genotypes based on multiple traits revealed two varieties with above-average seed yield per plant and four varieties with high oil and above average protein content, which could be used as potential parents in breeding for important agronomic traits in future breeding programs.",
journal = "Breeding Grasses and Protein Crops in the Era of Genomics",
booktitle = "Application of multivariate analysis for genotype evaluation in soybean",
pages = "215-219",
doi = "10.1007/978-3-319-89578-9_39",
url = "conv_1010"
}
Perić, V., Srebrić, M., Nikolić, A.,& Mladenović Drinić, S.. (2018). Application of multivariate analysis for genotype evaluation in soybean. in Breeding Grasses and Protein Crops in the Era of Genomics, 215-219.
https://doi.org/10.1007/978-3-319-89578-9_39
conv_1010
Perić V, Srebrić M, Nikolić A, Mladenović Drinić S. Application of multivariate analysis for genotype evaluation in soybean. in Breeding Grasses and Protein Crops in the Era of Genomics. 2018;:215-219.
doi:10.1007/978-3-319-89578-9_39
conv_1010 .
Perić, Vesna, Srebrić, Mirjana, Nikolić, Ana, Mladenović Drinić, Snežana, "Application of multivariate analysis for genotype evaluation in soybean" in Breeding Grasses and Protein Crops in the Era of Genomics (2018):215-219,
https://doi.org/10.1007/978-3-319-89578-9_39 .,
conv_1010 .

Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat

Nikolić, Milica; Nikolić, Ana; Savić, Iva; Petrović, Tanja; Stanković, Slavica; Jauković, Marko; Bagi, Ferenc

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Nikolić, Ana
AU  - Savić, Iva
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Jauković, Marko
AU  - Bagi, Ferenc
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/709
AB  - The species of the genus Aspergillus, A. flavus and A. parasiticus, are the most aflatoxin-producing fungi. All previous studies carried out under the production conditions of Serbia showed no presence of A. parasiticus on wheat kernel. On the basis of changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, which favour increased frequency of Aspergillus spp., we assumed that this pathogen can also be present in Serbia. The significance of direct losses as a consequence of wheat kernel infection, as well as potential contamination with aflatoxins, have pointed out to the need to determine the presence of toxigenic potential of A. flavus and A. parasiticus isolates originating from Serbia. For that purpose, wheat kernel samples were collected in nine locations. According to morphological, toxicological and molecular traits of isolated fungi, the presence of A. flavus and A. parasiticus was confirmed. This is the first time that A. parasiticus was identified on wheat under climatic conditions in Serbia. This study indicates that these pathogens may be a potential danger in wheat production in the region of Serbia. This danger will be much more certain if global climatic changes continue as they will provide more intensive development of these pathogens.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat
VL  - 50
IS  - 1
SP  - 143
EP  - 152
DO  - 10.2298/GENSR1801143N
UR  - conv_979
ER  - 
@article{
author = "Nikolić, Milica and Nikolić, Ana and Savić, Iva and Petrović, Tanja and Stanković, Slavica and Jauković, Marko and Bagi, Ferenc",
year = "2018",
abstract = "The species of the genus Aspergillus, A. flavus and A. parasiticus, are the most aflatoxin-producing fungi. All previous studies carried out under the production conditions of Serbia showed no presence of A. parasiticus on wheat kernel. On the basis of changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, which favour increased frequency of Aspergillus spp., we assumed that this pathogen can also be present in Serbia. The significance of direct losses as a consequence of wheat kernel infection, as well as potential contamination with aflatoxins, have pointed out to the need to determine the presence of toxigenic potential of A. flavus and A. parasiticus isolates originating from Serbia. For that purpose, wheat kernel samples were collected in nine locations. According to morphological, toxicological and molecular traits of isolated fungi, the presence of A. flavus and A. parasiticus was confirmed. This is the first time that A. parasiticus was identified on wheat under climatic conditions in Serbia. This study indicates that these pathogens may be a potential danger in wheat production in the region of Serbia. This danger will be much more certain if global climatic changes continue as they will provide more intensive development of these pathogens.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat",
volume = "50",
number = "1",
pages = "143-152",
doi = "10.2298/GENSR1801143N",
url = "conv_979"
}
Nikolić, M., Nikolić, A., Savić, I., Petrović, T., Stanković, S., Jauković, M.,& Bagi, F.. (2018). Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat. in Genetika
Društvo genetičara Srbije, Beograd., 50(1), 143-152.
https://doi.org/10.2298/GENSR1801143N
conv_979
Nikolić M, Nikolić A, Savić I, Petrović T, Stanković S, Jauković M, Bagi F. Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat. in Genetika. 2018;50(1):143-152.
doi:10.2298/GENSR1801143N
conv_979 .
Nikolić, Milica, Nikolić, Ana, Savić, Iva, Petrović, Tanja, Stanković, Slavica, Jauković, Marko, Bagi, Ferenc, "Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat" in Genetika, 50, no. 1 (2018):143-152,
https://doi.org/10.2298/GENSR1801143N .,
conv_979 .
4
5
4

Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions

Kandić, Vesna; Dodig, Dejan; Nikolić, Ana; Kaitović, Željko; Zorić, Miroslav; Šurlan-Momirović, Gordana; Aleksić, Goran; Đurić, Nenad

(Patron Editore S R L, Bologna, 2018)

TY  - JOUR
AU  - Kandić, Vesna
AU  - Dodig, Dejan
AU  - Nikolić, Ana
AU  - Kaitović, Željko
AU  - Zorić, Miroslav
AU  - Šurlan-Momirović, Gordana
AU  - Aleksić, Goran
AU  - Đurić, Nenad
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/730
AB  - After the kernel number per spike has been formed during the vegetative stage, grain weight depends on the rate and duration of grain filling. Drought and high temperatures during the grain filling stage frequently occurs in the majority of barley growing regions worldwide. To investigate impact of terminal drought stress on grain filling parameters and grain yield, 15 two- and 10 six-rowed barley genotypes were tested in two-year field trials, set in two locations with two treatments. One treatment was control (C), while in the other treatment (D) terminal drought was simulated by mechanical removal of all leaf blades 7 days after heading of each genotype. In average, defoliation caused reduction of yield (33%), grain filling rate (12%) and 1000-kernel weight (11%). In both treatments, grain yield and the absolute grain filling rate were higher in two-rowed barley genotypes than in six-rowed ones. In both treatments, the correlation was stronger between yield and grain filling duration than between yield and the grain filling rate. The correlation between average yield and grain filling duration was stronger in the D than in the C treatment. According to the results obtained for terminal drought conditions, breeders should create early maturing genotypes of two-rowed type, with a longer grain filling duration and the gradual accumulation of dry matter.
PB  - Patron Editore S R L, Bologna
T2  - Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia
T1  - Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions
VL  - 23
IS  - 2
SP  - 5
EP  - 14
DO  - 10.19199/2018.2.2038-5625.005
UR  - conv_987
ER  - 
@article{
author = "Kandić, Vesna and Dodig, Dejan and Nikolić, Ana and Kaitović, Željko and Zorić, Miroslav and Šurlan-Momirović, Gordana and Aleksić, Goran and Đurić, Nenad",
year = "2018",
abstract = "After the kernel number per spike has been formed during the vegetative stage, grain weight depends on the rate and duration of grain filling. Drought and high temperatures during the grain filling stage frequently occurs in the majority of barley growing regions worldwide. To investigate impact of terminal drought stress on grain filling parameters and grain yield, 15 two- and 10 six-rowed barley genotypes were tested in two-year field trials, set in two locations with two treatments. One treatment was control (C), while in the other treatment (D) terminal drought was simulated by mechanical removal of all leaf blades 7 days after heading of each genotype. In average, defoliation caused reduction of yield (33%), grain filling rate (12%) and 1000-kernel weight (11%). In both treatments, grain yield and the absolute grain filling rate were higher in two-rowed barley genotypes than in six-rowed ones. In both treatments, the correlation was stronger between yield and grain filling duration than between yield and the grain filling rate. The correlation between average yield and grain filling duration was stronger in the D than in the C treatment. According to the results obtained for terminal drought conditions, breeders should create early maturing genotypes of two-rowed type, with a longer grain filling duration and the gradual accumulation of dry matter.",
publisher = "Patron Editore S R L, Bologna",
journal = "Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia",
title = "Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions",
volume = "23",
number = "2",
pages = "5-14",
doi = "10.19199/2018.2.2038-5625.005",
url = "conv_987"
}
Kandić, V., Dodig, D., Nikolić, A., Kaitović, Ž., Zorić, M., Šurlan-Momirović, G., Aleksić, G.,& Đurić, N.. (2018). Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions. in Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia
Patron Editore S R L, Bologna., 23(2), 5-14.
https://doi.org/10.19199/2018.2.2038-5625.005
conv_987
Kandić V, Dodig D, Nikolić A, Kaitović Ž, Zorić M, Šurlan-Momirović G, Aleksić G, Đurić N. Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions. in Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia. 2018;23(2):5-14.
doi:10.19199/2018.2.2038-5625.005
conv_987 .
Kandić, Vesna, Dodig, Dejan, Nikolić, Ana, Kaitović, Željko, Zorić, Miroslav, Šurlan-Momirović, Gordana, Aleksić, Goran, Đurić, Nenad, "Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions" in Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia, 23, no. 2 (2018):5-14,
https://doi.org/10.19199/2018.2.2038-5625.005 .,
conv_987 .
6
7

Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation

Dodig, Dejan; Kandić, Vesna; Nikolić, Ana; Zorić, Miroslav; Nikolic-Dorić, Emilija; Mutavdzić, Beba; Perović, Dragan; Šurlan-Momirović, Gordana

(Csiro Publishing, Clayton, 2018)

TY  - JOUR
AU  - Dodig, Dejan
AU  - Kandić, Vesna
AU  - Nikolić, Ana
AU  - Zorić, Miroslav
AU  - Nikolic-Dorić, Emilija
AU  - Mutavdzić, Beba
AU  - Perović, Dragan
AU  - Šurlan-Momirović, Gordana
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/707
AB  - Barley (Hordeum vulgare L.) is often grown in sites with low rainfall and high temperature during grain filling. Because spike architecture is one of basic footprints of barley domestication, the importance of spikes in adaptation to different environments or abiotic stresses can be hypothesised. In order to compare different barley spike types in terms of kernel growth and yield components, we tested 15 two-row and 10 six-row winter genotypes in eight environments where terminal drought was simulated by defoliation at 7 days after heading (7 DAH). Control plants were grown intact. On average, two-row genotypes outyielded six-row genotypes by 17% under control conditions and 33% under simulated late drought. Observations of kernel dry weights from 7 DAH through to harvest maturity at 5-day intervals were regressed onto a measure of thermal time. After preliminary evaluation of four nonlinear (S-shaped) models for kernel dry-weight accumulation, the ordinary logistic model was deemed the most appropriate in most cases and was finally applied to all plant-growth curves. Four parameters were estimated from the logistic model. Whereas two earliness estimators (inflection point and thermal time needed to reach maximum kernel weight) were similar for the two barley types, maximum kernel weight (Y-max) and mean rate of kernel growth (RG) were higher (P lt 0.05) in two-row than in six-row barleys. Differences in Y-max and RG among six-row barley genotypes were greater between control and defoliation treatments than between years, whereas among two-row barley genotypes, differences between years were greater, suggesting better stability of six-row types and better drought tolerance of two-row types in the tested barley set.
PB  - Csiro Publishing, Clayton
T2  - Crop & Pasture Science
T1  - Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation
VL  - 69
IS  - 12
SP  - 1215
EP  - 1224
DO  - 10.1071/CP18336
UR  - conv_998
ER  - 
@article{
author = "Dodig, Dejan and Kandić, Vesna and Nikolić, Ana and Zorić, Miroslav and Nikolic-Dorić, Emilija and Mutavdzić, Beba and Perović, Dragan and Šurlan-Momirović, Gordana",
year = "2018",
abstract = "Barley (Hordeum vulgare L.) is often grown in sites with low rainfall and high temperature during grain filling. Because spike architecture is one of basic footprints of barley domestication, the importance of spikes in adaptation to different environments or abiotic stresses can be hypothesised. In order to compare different barley spike types in terms of kernel growth and yield components, we tested 15 two-row and 10 six-row winter genotypes in eight environments where terminal drought was simulated by defoliation at 7 days after heading (7 DAH). Control plants were grown intact. On average, two-row genotypes outyielded six-row genotypes by 17% under control conditions and 33% under simulated late drought. Observations of kernel dry weights from 7 DAH through to harvest maturity at 5-day intervals were regressed onto a measure of thermal time. After preliminary evaluation of four nonlinear (S-shaped) models for kernel dry-weight accumulation, the ordinary logistic model was deemed the most appropriate in most cases and was finally applied to all plant-growth curves. Four parameters were estimated from the logistic model. Whereas two earliness estimators (inflection point and thermal time needed to reach maximum kernel weight) were similar for the two barley types, maximum kernel weight (Y-max) and mean rate of kernel growth (RG) were higher (P lt 0.05) in two-row than in six-row barleys. Differences in Y-max and RG among six-row barley genotypes were greater between control and defoliation treatments than between years, whereas among two-row barley genotypes, differences between years were greater, suggesting better stability of six-row types and better drought tolerance of two-row types in the tested barley set.",
publisher = "Csiro Publishing, Clayton",
journal = "Crop & Pasture Science",
title = "Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation",
volume = "69",
number = "12",
pages = "1215-1224",
doi = "10.1071/CP18336",
url = "conv_998"
}
Dodig, D., Kandić, V., Nikolić, A., Zorić, M., Nikolic-Dorić, E., Mutavdzić, B., Perović, D.,& Šurlan-Momirović, G.. (2018). Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation. in Crop & Pasture Science
Csiro Publishing, Clayton., 69(12), 1215-1224.
https://doi.org/10.1071/CP18336
conv_998
Dodig D, Kandić V, Nikolić A, Zorić M, Nikolic-Dorić E, Mutavdzić B, Perović D, Šurlan-Momirović G. Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation. in Crop & Pasture Science. 2018;69(12):1215-1224.
doi:10.1071/CP18336
conv_998 .
Dodig, Dejan, Kandić, Vesna, Nikolić, Ana, Zorić, Miroslav, Nikolic-Dorić, Emilija, Mutavdzić, Beba, Perović, Dragan, Šurlan-Momirović, Gordana, "Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation" in Crop & Pasture Science, 69, no. 12 (2018):1215-1224,
https://doi.org/10.1071/CP18336 .,
conv_998 .
9
6
7

Disruption of genetic identity for genebank maize accessions during conservation

Babić, Vojka; Anđelković, Violeta; Nikolić, Ana; Milivojević, Marija; Srdić, Jelena; Popović, Aleksandar; Kravić, Natalija

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Babić, Vojka
AU  - Anđelković, Violeta
AU  - Nikolić, Ana
AU  - Milivojević, Marija
AU  - Srdić, Jelena
AU  - Popović, Aleksandar
AU  - Kravić, Natalija
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/658
AB  - Maintenance of the original accessions identity and integrity is one of the priorities among genebank activities. Different factors related to conservation may result in accessions disruption. Regeneration is the most frequent critical point in this process, due to bottlenecks, inbreeding, random genetic drift and unintentional mixing or contamination. On the other hand, genetic drift may occur due to seed viability loss. Therefore, it is very important to establish the balance between the frequency of regeneration and the duration of accession conservation. The aim of the present study was to estimate whether the identity of accessions regenerated after 27 years of medium-term conservation was disrupted. Phenotypic markers were applied on three Maize Research Institute "Zemun Polje" (MRIZP) genebank maize landraces (K2026, K768 and K86), differing in seed viability, kernel type and effective population size. It was estimated that, after the regeneration, there had been no significant changes in the landrace K2026. There were some parameters indicating that genetic drift had occurred in the landrace K768, and that there had been even a certain degree of inbreeding in the landrace K86. According to the results, accession K2026 could still be kept under the same ID number. Due to the genuine identity disruption, assignment of new ID numbers for K768 and K86 should be suggested.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Disruption of genetic identity for genebank maize accessions during conservation
VL  - 49
IS  - 3
SP  - 853
EP  - 864
DO  - 10.2298/GENSR1703853B
UR  - conv_969
ER  - 
@article{
author = "Babić, Vojka and Anđelković, Violeta and Nikolić, Ana and Milivojević, Marija and Srdić, Jelena and Popović, Aleksandar and Kravić, Natalija",
year = "2017",
abstract = "Maintenance of the original accessions identity and integrity is one of the priorities among genebank activities. Different factors related to conservation may result in accessions disruption. Regeneration is the most frequent critical point in this process, due to bottlenecks, inbreeding, random genetic drift and unintentional mixing or contamination. On the other hand, genetic drift may occur due to seed viability loss. Therefore, it is very important to establish the balance between the frequency of regeneration and the duration of accession conservation. The aim of the present study was to estimate whether the identity of accessions regenerated after 27 years of medium-term conservation was disrupted. Phenotypic markers were applied on three Maize Research Institute "Zemun Polje" (MRIZP) genebank maize landraces (K2026, K768 and K86), differing in seed viability, kernel type and effective population size. It was estimated that, after the regeneration, there had been no significant changes in the landrace K2026. There were some parameters indicating that genetic drift had occurred in the landrace K768, and that there had been even a certain degree of inbreeding in the landrace K86. According to the results, accession K2026 could still be kept under the same ID number. Due to the genuine identity disruption, assignment of new ID numbers for K768 and K86 should be suggested.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Disruption of genetic identity for genebank maize accessions during conservation",
volume = "49",
number = "3",
pages = "853-864",
doi = "10.2298/GENSR1703853B",
url = "conv_969"
}
Babić, V., Anđelković, V., Nikolić, A., Milivojević, M., Srdić, J., Popović, A.,& Kravić, N.. (2017). Disruption of genetic identity for genebank maize accessions during conservation. in Genetika
Društvo genetičara Srbije, Beograd., 49(3), 853-864.
https://doi.org/10.2298/GENSR1703853B
conv_969
Babić V, Anđelković V, Nikolić A, Milivojević M, Srdić J, Popović A, Kravić N. Disruption of genetic identity for genebank maize accessions during conservation. in Genetika. 2017;49(3):853-864.
doi:10.2298/GENSR1703853B
conv_969 .
Babić, Vojka, Anđelković, Violeta, Nikolić, Ana, Milivojević, Marija, Srdić, Jelena, Popović, Aleksandar, Kravić, Natalija, "Disruption of genetic identity for genebank maize accessions during conservation" in Genetika, 49, no. 3 (2017):853-864,
https://doi.org/10.2298/GENSR1703853B .,
conv_969 .

Relationship between genetic diversity and cold-tolerance of maize inbred lines

Milivojević, Marija; Nikolić, Ana; Marković, Ksenija; Filipović, Milomir; Vančetović, Jelena; Petrović, Tanja; Srdić, Jelena

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Milivojević, Marija
AU  - Nikolić, Ana
AU  - Marković, Ksenija
AU  - Filipović, Milomir
AU  - Vančetović, Jelena
AU  - Petrović, Tanja
AU  - Srdić, Jelena
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/692
AB  - Early maize sowing enables longer growing season with enhanced possibility of achieving higher and more stable yields, and better chances of avoiding summer droughts. For early sowing, cold-tolerant maize genotypes should be used. Breeding maize, tolerant to low temperatures, requires knowledge of genetic diversity and heterotic patterns of breeding material. The objective of this study was to determine genetic diversity of 15 ZP maize inbred lines applying the method of protein markers (UTLIEF method), and to establish correspondence between thus obtained classification with the results of cold test (CT) and field emergence (FE). During two production seasons (2011 and 2014), 15 maize inbred lines were self-pollinated. Pedigree data showed that material belongs to different maturity and heterotic groups. Cold tolerance was assessed in laboratory (2015) by cold test (7.5 degrees C, 10 days), and field trials on two locations during two successive years (2015, 2016). ZP maize inbred lines showed very good response to stressful conditions of CT and FE. Inbred lines with Lancaster background were more sensitive to low temperatures than inbred lines with BSSS and Iowa Dent background. Based on UTLIEF method two inbred lines with Lancaster background (ZPL 5 and ZPL 7) were grouped by cluster analysis together with Iowa Dent inbred lines, that also expressed better cold tolerance, and thus exceptional consent was achieved with the results of CT and FE. Classification of maize inbred lines based on UTLIEF method, followed by cluster analysis and PCA, showed good agreement with pedigree data, which points out that this method could be successfully applied for genetic classification of breeding material of a wide genetic background.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Relationship between genetic diversity and cold-tolerance of maize inbred lines
VL  - 49
IS  - 2
SP  - 635
EP  - 646
DO  - 10.2298/GENSR1702635M
UR  - conv_964
ER  - 
@article{
author = "Milivojević, Marija and Nikolić, Ana and Marković, Ksenija and Filipović, Milomir and Vančetović, Jelena and Petrović, Tanja and Srdić, Jelena",
year = "2017",
abstract = "Early maize sowing enables longer growing season with enhanced possibility of achieving higher and more stable yields, and better chances of avoiding summer droughts. For early sowing, cold-tolerant maize genotypes should be used. Breeding maize, tolerant to low temperatures, requires knowledge of genetic diversity and heterotic patterns of breeding material. The objective of this study was to determine genetic diversity of 15 ZP maize inbred lines applying the method of protein markers (UTLIEF method), and to establish correspondence between thus obtained classification with the results of cold test (CT) and field emergence (FE). During two production seasons (2011 and 2014), 15 maize inbred lines were self-pollinated. Pedigree data showed that material belongs to different maturity and heterotic groups. Cold tolerance was assessed in laboratory (2015) by cold test (7.5 degrees C, 10 days), and field trials on two locations during two successive years (2015, 2016). ZP maize inbred lines showed very good response to stressful conditions of CT and FE. Inbred lines with Lancaster background were more sensitive to low temperatures than inbred lines with BSSS and Iowa Dent background. Based on UTLIEF method two inbred lines with Lancaster background (ZPL 5 and ZPL 7) were grouped by cluster analysis together with Iowa Dent inbred lines, that also expressed better cold tolerance, and thus exceptional consent was achieved with the results of CT and FE. Classification of maize inbred lines based on UTLIEF method, followed by cluster analysis and PCA, showed good agreement with pedigree data, which points out that this method could be successfully applied for genetic classification of breeding material of a wide genetic background.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Relationship between genetic diversity and cold-tolerance of maize inbred lines",
volume = "49",
number = "2",
pages = "635-646",
doi = "10.2298/GENSR1702635M",
url = "conv_964"
}
Milivojević, M., Nikolić, A., Marković, K., Filipović, M., Vančetović, J., Petrović, T.,& Srdić, J.. (2017). Relationship between genetic diversity and cold-tolerance of maize inbred lines. in Genetika
Društvo genetičara Srbije, Beograd., 49(2), 635-646.
https://doi.org/10.2298/GENSR1702635M
conv_964
Milivojević M, Nikolić A, Marković K, Filipović M, Vančetović J, Petrović T, Srdić J. Relationship between genetic diversity and cold-tolerance of maize inbred lines. in Genetika. 2017;49(2):635-646.
doi:10.2298/GENSR1702635M
conv_964 .
Milivojević, Marija, Nikolić, Ana, Marković, Ksenija, Filipović, Milomir, Vančetović, Jelena, Petrović, Tanja, Srdić, Jelena, "Relationship between genetic diversity and cold-tolerance of maize inbred lines" in Genetika, 49, no. 2 (2017):635-646,
https://doi.org/10.2298/GENSR1702635M .,
conv_964 .
4
2
2

Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia

Obradović, Ana; Stanković, Slavica; Nikolić, Ana; Ignjatović-Micić, Dragana; Krnjaja, Vesna; Stepanović, Jelena; Duduk, Bojan

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Krnjaja, Vesna
AU  - Stepanović, Jelena
AU  - Duduk, Bojan
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/682
AB  - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia
VL  - 49
IS  - 1
SP  - 355
EP  - 364
DO  - 10.2298/GENSR1701355O
UR  - conv_957
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Nikolić, Ana and Ignjatović-Micić, Dragana and Krnjaja, Vesna and Stepanović, Jelena and Duduk, Bojan",
year = "2017",
abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia",
volume = "49",
number = "1",
pages = "355-364",
doi = "10.2298/GENSR1701355O",
url = "conv_957"
}
Obradović, A., Stanković, S., Nikolić, A., Ignjatović-Micić, D., Krnjaja, V., Stepanović, J.,& Duduk, B.. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 49(1), 355-364.
https://doi.org/10.2298/GENSR1701355O
conv_957
Obradović A, Stanković S, Nikolić A, Ignjatović-Micić D, Krnjaja V, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika. 2017;49(1):355-364.
doi:10.2298/GENSR1701355O
conv_957 .
Obradović, Ana, Stanković, Slavica, Nikolić, Ana, Ignjatović-Micić, Dragana, Krnjaja, Vesna, Stepanović, Jelena, Duduk, Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" in Genetika, 49, no. 1 (2017):355-364,
https://doi.org/10.2298/GENSR1701355O .,
conv_957 .
6
3
5

Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends

Vančetović, Jelena; Ignjatović-Micić, Dragana; Delić, Nenad; Božinović, Sofija; Nikolić, Ana; Kostadinović, Marija; Trbović, Dejana

(Univ Sao Paolo, Cerquera Cesar, 2017)

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Delić, Nenad
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Trbović, Dejana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/684
AB  - A pilot experiment was undertaken in order to examine high oil populations of maize (Zea mays L.) to be used as pollinators in TopCross blends with commercial ZP341 standard hybrid. Five high oil populations (HOPs) from the Maize Research Institute (MRI) gene bank were chosen for this research, according to their high grain oil content, synchrony between silking of ZP341 and anthesis of the populations and good agronomic performances in 2012. Selfing of ZP341 and HOPs, as well as crosses of ZP341 cmsS sterile x HOPs were carried out in 2013. Oil content, fatty acid composition, protein and tryptophan content, and physical characteristics of the obtained kernels were measured. Four HOPs showed significant positive influence on the oil content in the TopCrosses (TC), 16.85 g kg(-1) on average. Oleic acid, which is the principal monounsaturated fatty acid, was significantly lower in all HOPs and all TCs, while selfed ZP341 had almost twice the average value typical for standard maize. However, this decrease in TCs was in a narrow range from 1 % (in TC-3) to 5 % (in TC-4) and the oleic content of TCs was on average higher by 60 % compared to the typical standard maize. Different favorable and unfavorable significant changes were detected in fatty acid compositions, protein and tryptophan contents and physical kernel properties for each potential TC combination. Results indicate differences in gene effects present in different TC combinations and underscore the need to examine each potential TC blend by conducting similar simple experiments.
PB  - Univ Sao Paolo, Cerquera Cesar
T2  - Scientia Agricola
T1  - Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends
VL  - 74
IS  - 6
SP  - 461
EP  - 468
DO  - 10.1590/1678-992X-2016-0302
UR  - conv_959
ER  - 
@article{
author = "Vančetović, Jelena and Ignjatović-Micić, Dragana and Delić, Nenad and Božinović, Sofija and Nikolić, Ana and Kostadinović, Marija and Trbović, Dejana",
year = "2017",
abstract = "A pilot experiment was undertaken in order to examine high oil populations of maize (Zea mays L.) to be used as pollinators in TopCross blends with commercial ZP341 standard hybrid. Five high oil populations (HOPs) from the Maize Research Institute (MRI) gene bank were chosen for this research, according to their high grain oil content, synchrony between silking of ZP341 and anthesis of the populations and good agronomic performances in 2012. Selfing of ZP341 and HOPs, as well as crosses of ZP341 cmsS sterile x HOPs were carried out in 2013. Oil content, fatty acid composition, protein and tryptophan content, and physical characteristics of the obtained kernels were measured. Four HOPs showed significant positive influence on the oil content in the TopCrosses (TC), 16.85 g kg(-1) on average. Oleic acid, which is the principal monounsaturated fatty acid, was significantly lower in all HOPs and all TCs, while selfed ZP341 had almost twice the average value typical for standard maize. However, this decrease in TCs was in a narrow range from 1 % (in TC-3) to 5 % (in TC-4) and the oleic content of TCs was on average higher by 60 % compared to the typical standard maize. Different favorable and unfavorable significant changes were detected in fatty acid compositions, protein and tryptophan contents and physical kernel properties for each potential TC combination. Results indicate differences in gene effects present in different TC combinations and underscore the need to examine each potential TC blend by conducting similar simple experiments.",
publisher = "Univ Sao Paolo, Cerquera Cesar",
journal = "Scientia Agricola",
title = "Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends",
volume = "74",
number = "6",
pages = "461-468",
doi = "10.1590/1678-992X-2016-0302",
url = "conv_959"
}
Vančetović, J., Ignjatović-Micić, D., Delić, N., Božinović, S., Nikolić, A., Kostadinović, M.,& Trbović, D.. (2017). Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends. in Scientia Agricola
Univ Sao Paolo, Cerquera Cesar., 74(6), 461-468.
https://doi.org/10.1590/1678-992X-2016-0302
conv_959
Vančetović J, Ignjatović-Micić D, Delić N, Božinović S, Nikolić A, Kostadinović M, Trbović D. Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends. in Scientia Agricola. 2017;74(6):461-468.
doi:10.1590/1678-992X-2016-0302
conv_959 .
Vančetović, Jelena, Ignjatović-Micić, Dragana, Delić, Nenad, Božinović, Sofija, Nikolić, Ana, Kostadinović, Marija, Trbović, Dejana, "Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends" in Scientia Agricola, 74, no. 6 (2017):461-468,
https://doi.org/10.1590/1678-992X-2016-0302 .,
conv_959 .
2
2
2

Comparison of statistical methods for genetic similarity evaluation of maize inbred lines

Nikolić, Ana; Kostadinović, Marija; Vančetović, Jelena; Stanković, Goran; Ignjatović-Micić, Dragana

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2017)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Ignjatović-Micić, Dragana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/679
AB  - Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index.
AB  - Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Comparison of statistical methods for genetic similarity evaluation of maize inbred lines
T1  - Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza
VL  - 54
IS  - 1
SP  - 25
EP  - 30
DO  - 10.5937/ratpov54-12174
UR  - conv_623
ER  - 
@article{
author = "Nikolić, Ana and Kostadinović, Marija and Vančetović, Jelena and Stanković, Goran and Ignjatović-Micić, Dragana",
year = "2017",
abstract = "Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index., Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines, Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza",
volume = "54",
number = "1",
pages = "25-30",
doi = "10.5937/ratpov54-12174",
url = "conv_623"
}
Nikolić, A., Kostadinović, M., Vančetović, J., Stanković, G.,& Ignjatović-Micić, D.. (2017). Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 54(1), 25-30.
https://doi.org/10.5937/ratpov54-12174
conv_623
Nikolić A, Kostadinović M, Vančetović J, Stanković G, Ignjatović-Micić D. Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo. 2017;54(1):25-30.
doi:10.5937/ratpov54-12174
conv_623 .
Nikolić, Ana, Kostadinović, Marija, Vančetović, Jelena, Stanković, Goran, Ignjatović-Micić, Dragana, "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines" in Ratarstvo i povrtarstvo, 54, no. 1 (2017):25-30,
https://doi.org/10.5937/ratpov54-12174 .,
conv_623 .
1

DNA and biochemical analysis of a potential opaque2 maize population

Ignjatović-Micić, Dragana; Nikolić, Ana; Kostadinović, Marija; Nikolić, Aleksandra

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Nikolić, Aleksandra
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/680
AB  - Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples - with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of -IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - DNA and biochemical analysis of a potential opaque2 maize population
VL  - 49
IS  - 1
SP  - 51
EP  - 61
DO  - 10.2298/GENSR1701051I
UR  - conv_956
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Nikolić, Ana and Kostadinović, Marija and Nikolić, Aleksandra",
year = "2017",
abstract = "Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples - with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of -IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "DNA and biochemical analysis of a potential opaque2 maize population",
volume = "49",
number = "1",
pages = "51-61",
doi = "10.2298/GENSR1701051I",
url = "conv_956"
}
Ignjatović-Micić, D., Nikolić, A., Kostadinović, M.,& Nikolić, A.. (2017). DNA and biochemical analysis of a potential opaque2 maize population. in Genetika
Društvo genetičara Srbije, Beograd., 49(1), 51-61.
https://doi.org/10.2298/GENSR1701051I
conv_956
Ignjatović-Micić D, Nikolić A, Kostadinović M, Nikolić A. DNA and biochemical analysis of a potential opaque2 maize population. in Genetika. 2017;49(1):51-61.
doi:10.2298/GENSR1701051I
conv_956 .
Ignjatović-Micić, Dragana, Nikolić, Ana, Kostadinović, Marija, Nikolić, Aleksandra, "DNA and biochemical analysis of a potential opaque2 maize population" in Genetika, 49, no. 1 (2017):51-61,
https://doi.org/10.2298/GENSR1701051I .,
conv_956 .

Establishment and confirmation of heterotic groups and genetic diversity assessment of maize inbred lines using microsatellite data

Nikolić, Ana; Bogosavljević, Jelena; Čamdžija, Zoran; Filipović, Milomir; Kovačević, Dragan; Stevanović, Milan; Mladenović Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2016)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Bogosavljević, Jelena
AU  - Čamdžija, Zoran
AU  - Filipović, Milomir
AU  - Kovačević, Dragan
AU  - Stevanović, Milan
AU  - Mladenović Drinić, Snežana
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/637
AB  - Twenty-seven maize inbreds (12 commercial and 15 developing lines) from Maize Research Institute breeding program were subjected to microsatelite analysis. The aim was genetic diversity determination, establishing relationships among tested lines and assigning them to heterotic groups according to molecular marker data. Number of alelles detected was 97, with an average of 3.23. Major allele frequency was in a range from 0.33 to 0.82 (average 0.55). The highest value for observed heterozygosity was 10% for several developing lines. Mean values for gene diversity and PIC were 0.56 and 0.48, respectively. Frequency-based distances were calculated using Roger's coefficient and average value of 0.57 indicates high genetic diversity in analyzed maize inbreds. Distance matrices were subjected to cluster analysis and PCA. Multivariate analysis methods showed considerable concurrency with pedigree data. Results of analysis with 30 microsatellite markers could be useful for defining/redefining heterotic groups but should be complemented with field testing data.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Establishment and confirmation of heterotic groups and genetic diversity assessment of maize inbred lines using microsatellite data
VL  - 48
IS  - 3
SP  - 1067
EP  - 1076
DO  - 10.2298/GENSR1603067N
UR  - conv_945
ER  - 
@article{
author = "Nikolić, Ana and Bogosavljević, Jelena and Čamdžija, Zoran and Filipović, Milomir and Kovačević, Dragan and Stevanović, Milan and Mladenović Drinić, Snežana",
year = "2016",
abstract = "Twenty-seven maize inbreds (12 commercial and 15 developing lines) from Maize Research Institute breeding program were subjected to microsatelite analysis. The aim was genetic diversity determination, establishing relationships among tested lines and assigning them to heterotic groups according to molecular marker data. Number of alelles detected was 97, with an average of 3.23. Major allele frequency was in a range from 0.33 to 0.82 (average 0.55). The highest value for observed heterozygosity was 10% for several developing lines. Mean values for gene diversity and PIC were 0.56 and 0.48, respectively. Frequency-based distances were calculated using Roger's coefficient and average value of 0.57 indicates high genetic diversity in analyzed maize inbreds. Distance matrices were subjected to cluster analysis and PCA. Multivariate analysis methods showed considerable concurrency with pedigree data. Results of analysis with 30 microsatellite markers could be useful for defining/redefining heterotic groups but should be complemented with field testing data.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Establishment and confirmation of heterotic groups and genetic diversity assessment of maize inbred lines using microsatellite data",
volume = "48",
number = "3",
pages = "1067-1076",
doi = "10.2298/GENSR1603067N",
url = "conv_945"
}
Nikolić, A., Bogosavljević, J., Čamdžija, Z., Filipović, M., Kovačević, D., Stevanović, M.,& Mladenović Drinić, S.. (2016). Establishment and confirmation of heterotic groups and genetic diversity assessment of maize inbred lines using microsatellite data. in Genetika
Društvo genetičara Srbije, Beograd., 48(3), 1067-1076.
https://doi.org/10.2298/GENSR1603067N
conv_945
Nikolić A, Bogosavljević J, Čamdžija Z, Filipović M, Kovačević D, Stevanović M, Mladenović Drinić S. Establishment and confirmation of heterotic groups and genetic diversity assessment of maize inbred lines using microsatellite data. in Genetika. 2016;48(3):1067-1076.
doi:10.2298/GENSR1603067N
conv_945 .
Nikolić, Ana, Bogosavljević, Jelena, Čamdžija, Zoran, Filipović, Milomir, Kovačević, Dragan, Stevanović, Milan, Mladenović Drinić, Snežana, "Establishment and confirmation of heterotic groups and genetic diversity assessment of maize inbred lines using microsatellite data" in Genetika, 48, no. 3 (2016):1067-1076,
https://doi.org/10.2298/GENSR1603067N .,
conv_945 .
2
2

UPOV morphological versus molecular markers for maize inbred lines variability determination

Babić, Vojka; Nikolić, Ana; Anđelković, Violeta; Kovačević, Dragan; Filipović, Milomir; Mladenović Drinić, Snežana; Vasić, Vladimir

(Inst Investigaciones Agropecuarias, Chillan, 2016)

TY  - JOUR
AU  - Babić, Vojka
AU  - Nikolić, Ana
AU  - Anđelković, Violeta
AU  - Kovačević, Dragan
AU  - Filipović, Milomir
AU  - Mladenović Drinić, Snežana
AU  - Vasić, Vladimir
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/625
AB  - In maize (Zen mays L.) breeding programs, it is very important to define a genetic distance of inbred lines and to establish criteria and biometric methods for the satisfactory germplasm classification. A total of 29 inbred lines from Maize Research Institute "Zemun Polje" (MRIZP) breeding program, Belgrade, Serbia, were used to compare similarities obtained by morphological (according to UPOV- Union Internationale pour la Protection des Obtentions Vegetales) and molecular (simple sequence repeats, SSR) markers. The aim was to assess discriminative power of applied markers in the separation of related lines, and to determine the possibility of their application in choosing parental lines for breeding programs. Application of different marker types resulted in adequate separation of inbreds into divergent groups, which facilitates the choice of parental lines. However, there were still many inconsistencies between the field and laboratory results. The quality of information from morphological markers was improved by the application of the appropriate descriptor, measurement scale and the biometric method. According to the correspondence analysis, increased number of SSR markers will enable more reliable results. Regression analysis of morphological visual assessment (MVG) and SSR similarity of individual inbreds pointed out that in some cases morphological, and in others molecular markers more accurately reflected known pedigree information of tested maize lines. In the early generations of maize inbred lines testing, we propose utilization of information resulting from in orphologi cal markers, according to UPOV descriptor. However, application of adequate number of molecular (SSR) markers has an economic justification on a smaller number of elite lines in the later generations of line testing.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - UPOV morphological versus molecular markers for maize inbred lines variability determination
VL  - 76
IS  - 4
DO  - 10.4067/S0718-58392016000400004
UR  - conv_948
ER  - 
@article{
author = "Babić, Vojka and Nikolić, Ana and Anđelković, Violeta and Kovačević, Dragan and Filipović, Milomir and Mladenović Drinić, Snežana and Vasić, Vladimir",
year = "2016",
abstract = "In maize (Zen mays L.) breeding programs, it is very important to define a genetic distance of inbred lines and to establish criteria and biometric methods for the satisfactory germplasm classification. A total of 29 inbred lines from Maize Research Institute "Zemun Polje" (MRIZP) breeding program, Belgrade, Serbia, were used to compare similarities obtained by morphological (according to UPOV- Union Internationale pour la Protection des Obtentions Vegetales) and molecular (simple sequence repeats, SSR) markers. The aim was to assess discriminative power of applied markers in the separation of related lines, and to determine the possibility of their application in choosing parental lines for breeding programs. Application of different marker types resulted in adequate separation of inbreds into divergent groups, which facilitates the choice of parental lines. However, there were still many inconsistencies between the field and laboratory results. The quality of information from morphological markers was improved by the application of the appropriate descriptor, measurement scale and the biometric method. According to the correspondence analysis, increased number of SSR markers will enable more reliable results. Regression analysis of morphological visual assessment (MVG) and SSR similarity of individual inbreds pointed out that in some cases morphological, and in others molecular markers more accurately reflected known pedigree information of tested maize lines. In the early generations of maize inbred lines testing, we propose utilization of information resulting from in orphologi cal markers, according to UPOV descriptor. However, application of adequate number of molecular (SSR) markers has an economic justification on a smaller number of elite lines in the later generations of line testing.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "UPOV morphological versus molecular markers for maize inbred lines variability determination",
volume = "76",
number = "4",
doi = "10.4067/S0718-58392016000400004",
url = "conv_948"
}
Babić, V., Nikolić, A., Anđelković, V., Kovačević, D., Filipović, M., Mladenović Drinić, S.,& Vasić, V.. (2016). UPOV morphological versus molecular markers for maize inbred lines variability determination. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 76(4).
https://doi.org/10.4067/S0718-58392016000400004
conv_948
Babić V, Nikolić A, Anđelković V, Kovačević D, Filipović M, Mladenović Drinić S, Vasić V. UPOV morphological versus molecular markers for maize inbred lines variability determination. in Chilean Journal of Agricultural Research. 2016;76(4).
doi:10.4067/S0718-58392016000400004
conv_948 .
Babić, Vojka, Nikolić, Ana, Anđelković, Violeta, Kovačević, Dragan, Filipović, Milomir, Mladenović Drinić, Snežana, Vasić, Vladimir, "UPOV morphological versus molecular markers for maize inbred lines variability determination" in Chilean Journal of Agricultural Research, 76, no. 4 (2016),
https://doi.org/10.4067/S0718-58392016000400004 .,
conv_948 .
12
5
10

Genetic diversity of maize inbred lines as inferred from SSR markers

Nikolić, Ana; Ignjatović-Micić, Dragana; Kovačević, Dragan; Čamdžija, Zoran; Filipović, Milomir; Mladenović Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2015)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Kovačević, Dragan
AU  - Čamdžija, Zoran
AU  - Filipović, Milomir
AU  - Mladenović Drinić, Snežana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/585
AB  - Creating new maize hybrids with greater yield potential is a permanent goal of breeding programs all over the world. Long-time existing and new problems related to different biotic and abiotic stresses and the growing needs of the world market require constant work on finding new ways for advancing maize production. Molecular marker technology is one of the fastest developing fields and its implementation has already given results in solving different problems related to maize breeding improvement. The aim of the study presented herein was characterization and genetic similarity assessment of twenty-nine maize inbred lines from Maize Research Institute collection using Simple Sequence Repeats (SSR) markers. The analysis was done using 20 pairs of SSR primers with clearly visible and reproducible results. A total of 119 alleles were detected with a mean of 5.8 per locus. PIC (Polymorphism Information Content) values were in the range from 0.45 to 0.92 (average 0.74). Genetic similarities calculated using Jaccard's coefficient ranged from 0.27 to 0.99. Cluster and Principal Component Analysis (PCA) analysis were done using matrices of similarity in the NTSYSpc software, version 2.1. Results of both classifications were moderately in agreement with the pedigree data of analysed genotypes. The information about genetic diversity of maize inbred lines revealed by SSR markers could be useful in planning strategies for future maize breeding programs.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic diversity of maize inbred lines as inferred from SSR markers
VL  - 47
IS  - 2
SP  - 489
EP  - 498
DO  - 10.2298/GENSR1502489N
UR  - conv_916
ER  - 
@article{
author = "Nikolić, Ana and Ignjatović-Micić, Dragana and Kovačević, Dragan and Čamdžija, Zoran and Filipović, Milomir and Mladenović Drinić, Snežana",
year = "2015",
abstract = "Creating new maize hybrids with greater yield potential is a permanent goal of breeding programs all over the world. Long-time existing and new problems related to different biotic and abiotic stresses and the growing needs of the world market require constant work on finding new ways for advancing maize production. Molecular marker technology is one of the fastest developing fields and its implementation has already given results in solving different problems related to maize breeding improvement. The aim of the study presented herein was characterization and genetic similarity assessment of twenty-nine maize inbred lines from Maize Research Institute collection using Simple Sequence Repeats (SSR) markers. The analysis was done using 20 pairs of SSR primers with clearly visible and reproducible results. A total of 119 alleles were detected with a mean of 5.8 per locus. PIC (Polymorphism Information Content) values were in the range from 0.45 to 0.92 (average 0.74). Genetic similarities calculated using Jaccard's coefficient ranged from 0.27 to 0.99. Cluster and Principal Component Analysis (PCA) analysis were done using matrices of similarity in the NTSYSpc software, version 2.1. Results of both classifications were moderately in agreement with the pedigree data of analysed genotypes. The information about genetic diversity of maize inbred lines revealed by SSR markers could be useful in planning strategies for future maize breeding programs.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic diversity of maize inbred lines as inferred from SSR markers",
volume = "47",
number = "2",
pages = "489-498",
doi = "10.2298/GENSR1502489N",
url = "conv_916"
}
Nikolić, A., Ignjatović-Micić, D., Kovačević, D., Čamdžija, Z., Filipović, M.,& Mladenović Drinić, S.. (2015). Genetic diversity of maize inbred lines as inferred from SSR markers. in Genetika
Društvo genetičara Srbije, Beograd., 47(2), 489-498.
https://doi.org/10.2298/GENSR1502489N
conv_916
Nikolić A, Ignjatović-Micić D, Kovačević D, Čamdžija Z, Filipović M, Mladenović Drinić S. Genetic diversity of maize inbred lines as inferred from SSR markers. in Genetika. 2015;47(2):489-498.
doi:10.2298/GENSR1502489N
conv_916 .
Nikolić, Ana, Ignjatović-Micić, Dragana, Kovačević, Dragan, Čamdžija, Zoran, Filipović, Milomir, Mladenović Drinić, Snežana, "Genetic diversity of maize inbred lines as inferred from SSR markers" in Genetika, 47, no. 2 (2015):489-498,
https://doi.org/10.2298/GENSR1502489N .,
conv_916 .
1
5
5