Saratlić, Goran

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  • Saratlić, Goran (6)

Author's Bibliography

Characterization of sunflower seed and kernel proteins

Žilić, Slađana; Barać, Miroljub; Pešić, Mirjana; Crevar, Miloš; Stanojević, Slađana; Kovinčić, Anika; Saratlić, Goran; Tolimir, Miodrag

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2010)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Barać, Miroljub
AU  - Pešić, Mirjana
AU  - Crevar, Miloš
AU  - Stanojević, Slađana
AU  - Kovinčić, Anika
AU  - Saratlić, Goran
AU  - Tolimir, Miodrag
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/292
AB  - Total sunflower proteins, storage proteins, and helianthinin (11S) and 2S albumin fractions and their respective subunits in seeds and kernels of three sunflower hybrids were analyzed. Protein contents were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and coupled with densitometry. The SDS-PAGE profiles of the seed and kernel proteins in the crude extracts for all genotypes showed a very similar number of protein bands (thirty two) in the electrophoretograms. Three polypeptide groups of helianthinin fraction were detected. Two of these were acidic (α, Mw = 36,800 - 42,900 Da and α', Mw = 31,000 - 35,300 Da), while one was basic (β, Mw=21,000 - 29,600 Da). The molecular weight of the 2S albumin proteins ranged from 11,500 to 20,100 Da. According to our results, there were significant differences among the seed and kernel protein contents. The 2S albumin content was significantly higher in kernels than in whole seeds of sunflower hybrids (P lt 0.05). By contrast, the 11S helianthinin content was significantly higher in seeds (where it ranged from 61.75 to 67.70% of totally extracted proteins) than in kernels (varied from 57.36 to 61.51% of totally extracted proteins) of sunflower hybrids (P lt 0.05).
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - Characterization of sunflower seed and kernel proteins
VL  - 33
IS  - 52
SP  - 103
EP  - 113
DO  - 10.2298/HEL1052103Z
ER  - 
@article{
author = "Žilić, Slađana and Barać, Miroljub and Pešić, Mirjana and Crevar, Miloš and Stanojević, Slađana and Kovinčić, Anika and Saratlić, Goran and Tolimir, Miodrag",
year = "2010",
url = "http://rik.mrizp.rs/handle/123456789/292",
abstract = "Total sunflower proteins, storage proteins, and helianthinin (11S) and 2S albumin fractions and their respective subunits in seeds and kernels of three sunflower hybrids were analyzed. Protein contents were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and coupled with densitometry. The SDS-PAGE profiles of the seed and kernel proteins in the crude extracts for all genotypes showed a very similar number of protein bands (thirty two) in the electrophoretograms. Three polypeptide groups of helianthinin fraction were detected. Two of these were acidic (α, Mw = 36,800 - 42,900 Da and α', Mw = 31,000 - 35,300 Da), while one was basic (β, Mw=21,000 - 29,600 Da). The molecular weight of the 2S albumin proteins ranged from 11,500 to 20,100 Da. According to our results, there were significant differences among the seed and kernel protein contents. The 2S albumin content was significantly higher in kernels than in whole seeds of sunflower hybrids (P lt 0.05). By contrast, the 11S helianthinin content was significantly higher in seeds (where it ranged from 61.75 to 67.70% of totally extracted proteins) than in kernels (varied from 57.36 to 61.51% of totally extracted proteins) of sunflower hybrids (P lt 0.05).",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Characterization of sunflower seed and kernel proteins",
volume = "33",
number = "52",
pages = "103-113",
doi = "10.2298/HEL1052103Z"
}
Žilić, S., Barać, M., Pešić, M., Crevar, M., Stanojević, S., Kovinčić, A., Saratlić, G.,& Tolimir, M. (2010). Characterization of sunflower seed and kernel proteins.
Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 33(52), 103-113.
https://doi.org/10.2298/HEL1052103Z
Žilić S, Barać M, Pešić M, Crevar M, Stanojević S, Kovinčić A, Saratlić G, Tolimir M. Characterization of sunflower seed and kernel proteins. Helia. 2010;33(52):103-113
Žilić Slađana, Barać Miroljub, Pešić Mirjana, Crevar Miloš, Stanojević Slađana, Kovinčić Anika, Saratlić Goran, Tolimir Miodrag, "Characterization of sunflower seed and kernel proteins" Helia, 33, no. 52 (2010):103-113,
https://doi.org/10.2298/HEL1052103Z .
13
17

Protein and tryptophan content in kernels of maize hybrids

Žilić, Slađana; Ignjatović-Micić, Dragana; Saratlić, Goran; Stanković, Goran; Delić, Nenad; Filipović, Milomir; Kresović, Branka

(Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad, 2010)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Ignjatović-Micić, Dragana
AU  - Saratlić, Goran
AU  - Stanković, Goran
AU  - Delić, Nenad
AU  - Filipović, Milomir
AU  - Kresović, Branka
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/320
AB  - In this work, proteins and tryptophan content were analyzed in kernels of semi-flint, semi-dent, dent and specialty ZP maize hybrids. Protein content, as well as protein fraction, differed significantly among the analyzed maize genotypes. Globulin was the lowest fraction in kernels of all maize hybrids (6.69-11.03% of total protein). The content of albumin was the highest in sweet hybrids ZP 441su and ZP 461su i.e. 20.27% and 19.76% of total protein, respectively. α-Zein and G-3 glutelin were dominant protein fractions in all maize genotypes. The highest content of α-zein and G-3 glutelin was in the kernels of popping maize hybrid ZP 611k, i.e. 29.25 and 25.71% of total protein, respectively. All hybrids could be classified according to the tryptophan content in three groups - over 0.08% (sweet and waxy hybrids), from 0.06 to 0.07% (three standard dent hybrids, one semi-flint and two semident hybrid) and from 0.05 to 0.06% (two semi-flint, one semi-dent hybrids and popping).
AB  - U ovom radu ispitivan je sadržaj rastvorljivih proteina i triptofana u zrnu polutvrdunaca, poluzubana, zubana i specifičnih ZP hibrida kukuruza. Sadržaj ukupnih proteina, kao i sadržaj rastvorljivih proteinskih frakcija se statistički razlikovao između analiziranih genotipova kukuruza. Globulin je bio najmanjie zastupljena frakcija u kukuruznom zrnu (6.69-11.03% od ukupnih proteina). Sadržaj albuminske frakcije u zrnu hibrida šećeraca ZP 441su i ZP 461su iznosio je 20.27% i 19.76% od ukupnih proteina, dok je u zrnu ostalih hibrida bio značajno niži. α-zein i G3-glutelin bile su dominantne frakcije u zrnu svih ispitivanih genotipova kukuruza. Najviši sadržaj α-zeina i G3-glutelina bio je u zrnu kukuruza kokičara ZP 611k i iznosio je 29.25% i 25.71% od ukupnih proteina. Prema sadržaju triptofana svi analizirani hibridi mogu se podeliti u tri grupe: preko 0.08% (šećerci i voksi hibridi - ZP 441su, ZP 461su i ZP 704wx), od 0.06 do 0.07% (tri standardna zubana - ZP 434, ZP 544, ZP 688, poluzubani - ZP 643, ZP 717 i polutvrdunac ZP Prado), od 0.05 do 0.06% (dva polutvrdunca - ZP 209, ZP 745, jedan poluzuban i kokičar - ZP 505, ZP 611k). PR Projekat Ministarstva nauke Republike Srbije, br. TR-20039.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Protein and tryptophan content in kernels of maize hybrids
T1  - Sadržaj proteina i triptofana u zrnu hibrida kukuruza
VL  - 59
IS  - 1-2
SP  - 56
EP  - 64
ER  - 
@article{
author = "Žilić, Slađana and Ignjatović-Micić, Dragana and Saratlić, Goran and Stanković, Goran and Delić, Nenad and Filipović, Milomir and Kresović, Branka",
year = "2010",
url = "http://rik.mrizp.rs/handle/123456789/320",
abstract = "In this work, proteins and tryptophan content were analyzed in kernels of semi-flint, semi-dent, dent and specialty ZP maize hybrids. Protein content, as well as protein fraction, differed significantly among the analyzed maize genotypes. Globulin was the lowest fraction in kernels of all maize hybrids (6.69-11.03% of total protein). The content of albumin was the highest in sweet hybrids ZP 441su and ZP 461su i.e. 20.27% and 19.76% of total protein, respectively. α-Zein and G-3 glutelin were dominant protein fractions in all maize genotypes. The highest content of α-zein and G-3 glutelin was in the kernels of popping maize hybrid ZP 611k, i.e. 29.25 and 25.71% of total protein, respectively. All hybrids could be classified according to the tryptophan content in three groups - over 0.08% (sweet and waxy hybrids), from 0.06 to 0.07% (three standard dent hybrids, one semi-flint and two semident hybrid) and from 0.05 to 0.06% (two semi-flint, one semi-dent hybrids and popping)., U ovom radu ispitivan je sadržaj rastvorljivih proteina i triptofana u zrnu polutvrdunaca, poluzubana, zubana i specifičnih ZP hibrida kukuruza. Sadržaj ukupnih proteina, kao i sadržaj rastvorljivih proteinskih frakcija se statistički razlikovao između analiziranih genotipova kukuruza. Globulin je bio najmanjie zastupljena frakcija u kukuruznom zrnu (6.69-11.03% od ukupnih proteina). Sadržaj albuminske frakcije u zrnu hibrida šećeraca ZP 441su i ZP 461su iznosio je 20.27% i 19.76% od ukupnih proteina, dok je u zrnu ostalih hibrida bio značajno niži. α-zein i G3-glutelin bile su dominantne frakcije u zrnu svih ispitivanih genotipova kukuruza. Najviši sadržaj α-zeina i G3-glutelina bio je u zrnu kukuruza kokičara ZP 611k i iznosio je 29.25% i 25.71% od ukupnih proteina. Prema sadržaju triptofana svi analizirani hibridi mogu se podeliti u tri grupe: preko 0.08% (šećerci i voksi hibridi - ZP 441su, ZP 461su i ZP 704wx), od 0.06 do 0.07% (tri standardna zubana - ZP 434, ZP 544, ZP 688, poluzubani - ZP 643, ZP 717 i polutvrdunac ZP Prado), od 0.05 do 0.06% (dva polutvrdunca - ZP 209, ZP 745, jedan poluzuban i kokičar - ZP 505, ZP 611k). PR Projekat Ministarstva nauke Republike Srbije, br. TR-20039.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Protein and tryptophan content in kernels of maize hybrids, Sadržaj proteina i triptofana u zrnu hibrida kukuruza",
volume = "59",
number = "1-2",
pages = "56-64"
}
Žilić, S., Ignjatović-Micić, D., Saratlić, G., Stanković, G., Delić, N., Filipović, M.,& Kresović, B. (2010). Sadržaj proteina i triptofana u zrnu hibrida kukuruza.
Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 59(1-2), 56-64.
Žilić S, Ignjatović-Micić D, Saratlić G, Stanković G, Delić N, Filipović M, Kresović B. Sadržaj proteina i triptofana u zrnu hibrida kukuruza. Savremena poljoprivreda. 2010;59(1-2):56-64
Žilić Slađana, Ignjatović-Micić Dragana, Saratlić Goran, Stanković Goran, Delić Nenad, Filipović Milomir, Kresović Branka, "Sadržaj proteina i triptofana u zrnu hibrida kukuruza" Savremena poljoprivreda, 59, no. 1-2 (2010):56-64

Bread and durum wheat compared for antioxidants contents, and lipoxygenase and peroxidase activities

Žilić, Slađana; Dodig, Dejan; Saratlić, Goran; Hadži-Tašković-Šukalović, Vesna; Maksimović, Milan; Škrbić, Biljana

(Wiley-Blackwell, Hoboken, 2010)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Dodig, Dejan
AU  - Saratlić, Goran
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Maksimović, Milan
AU  - Škrbić, Biljana
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/341
AB  - P>Potential beneficial components, including proteins, total phenolics, total flavonoids, carotenoids, tocopherols, and DPPH radical scavenging activity, were investigated in wholemeal of ten bread (T. aestivum L.) and ten durum (T. durum Desf.) novel wheat genotypes. In addition, the activity rate of lipoxygenase (LOX) and peroxidase (POD) enzymes implicated in the antioxidant metabolism was determined. The protein contnet and the antioxidant properties varied according to the two different wheat species, as well as, between the different bread and durum wheat genotypes themselves. The results indicated significant differences in proteins and antioxidant compounds between bread and durum wheat. Higher total proteins, wet gluten and antioxidants contents, combined with lower LOX and POD activities, point to a higher nutritive value of durum wheat than bread wheat.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Food Science and Technology
T1  - Bread and durum wheat compared for antioxidants contents, and lipoxygenase and peroxidase activities
VL  - 45
IS  - 7
SP  - 1360
EP  - 1367
DO  - 10.1111/j.1365-2621.2010.02251.x
ER  - 
@article{
author = "Žilić, Slađana and Dodig, Dejan and Saratlić, Goran and Hadži-Tašković-Šukalović, Vesna and Maksimović, Milan and Škrbić, Biljana",
year = "2010",
url = "http://rik.mrizp.rs/handle/123456789/341",
abstract = "P>Potential beneficial components, including proteins, total phenolics, total flavonoids, carotenoids, tocopherols, and DPPH radical scavenging activity, were investigated in wholemeal of ten bread (T. aestivum L.) and ten durum (T. durum Desf.) novel wheat genotypes. In addition, the activity rate of lipoxygenase (LOX) and peroxidase (POD) enzymes implicated in the antioxidant metabolism was determined. The protein contnet and the antioxidant properties varied according to the two different wheat species, as well as, between the different bread and durum wheat genotypes themselves. The results indicated significant differences in proteins and antioxidant compounds between bread and durum wheat. Higher total proteins, wet gluten and antioxidants contents, combined with lower LOX and POD activities, point to a higher nutritive value of durum wheat than bread wheat.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Food Science and Technology",
title = "Bread and durum wheat compared for antioxidants contents, and lipoxygenase and peroxidase activities",
volume = "45",
number = "7",
pages = "1360-1367",
doi = "10.1111/j.1365-2621.2010.02251.x"
}
Žilić, S., Dodig, D., Saratlić, G., Hadži-Tašković-Šukalović, V., Maksimović, M.,& Škrbić, B. (2010). Bread and durum wheat compared for antioxidants contents, and lipoxygenase and peroxidase activities.
International Journal of Food Science and Technology
Wiley-Blackwell, Hoboken., 45(7), 1360-1367.
https://doi.org/10.1111/j.1365-2621.2010.02251.x
Žilić S, Dodig D, Saratlić G, Hadži-Tašković-Šukalović V, Maksimović M, Škrbić B. Bread and durum wheat compared for antioxidants contents, and lipoxygenase and peroxidase activities. International Journal of Food Science and Technology. 2010;45(7):1360-1367
Žilić Slađana, Dodig Dejan, Saratlić Goran, Hadži-Tašković-Šukalović Vesna, Maksimović Milan, Škrbić Biljana, "Bread and durum wheat compared for antioxidants contents, and lipoxygenase and peroxidase activities" International Journal of Food Science and Technology, 45, no. 7 (2010):1360-1367,
https://doi.org/10.1111/j.1365-2621.2010.02251.x .
15
16
21

The improved photosynthetic-fluorescence method and its application in breeding and seed production of maize

Radenović, Čedomir; Saratlić, Goran; Anđelković, Violeta; Radojčić, Aleksandar; Selaković, Dragojlo; Hojka, Zdravko

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2007)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Saratlić, Goran
AU  - Anđelković, Violeta
AU  - Radojčić, Aleksandar
AU  - Selaković, Dragojlo
AU  - Hojka, Zdravko
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/166
AB  - The application of the improved non-invasive photosynthetic-fluorescence method in maize breeding and seed production was observed in order to assess the resistance and adaptability of maize inbred lines to higher and high temperatures, as well as to drought. The studies were carried out with two maize inbred lines (ZPPL 115 and A 671). The thermal characteristics of the photosynthetic apparatus of the investigated maize inbred lines were determined as follows: temperature dependency range from 25 to 60oC, critical temperatures at which phase transitions take place in the thylakoid membrane, causing significant structural and functional changes in the photosynthetic apparatus of the investigated inbred lines, activation energies (Ea, kJ/mol) were calculated along the straight lines before and after the occurrence of the critical temperature. The activation energy (Ea) is a measure of the intensity of recombining responses to chlorophyll DF, and total changes in the structure and functioning of the thylakoid membranes. The results obtained and presented parameters of total photosynthetic and thermal processes of chlorophyll DF may contribute to a more accurate characterisation of maize inbred lines in respect to their resistance and adaptability to temperatures and drought. Consecutively, this can lead to a quicker and more rational improvement maize breeding and seed production.
AB  - Poboljšani neinvazivni fotosintetično-fluorescentni metod kandiduje se za primenu u oplemenjivanju i semenarstvu radi utvrđivanja ocene samooplodnih linija kukuruza na plastičnost, tolerantnost, otpornost i adaptivnost prema višim i visokim temperaturama, kao i prema suši. Utvrđene su termalne karakteristike fotosintetičnog aparata proučavanih samooplodnih linija kukuruza, i to: određena je temperaturna zavisnost u opsegu od 25 do 60oC, otkrivene su kritične temperature na kojima dolazi do faznih transformacija u tilakoidnoj membrani, a time i do značajnih strukturnih i funkcionalnih promena u fotosintetičnom aparatu ispitivanih samooplodnih linija kukuruza, obračunate su energije aktivacije (Ea, kJ/mol) duž pravih linija pre i posle pojavljivanja kritične temperature. Energija aktivacije (Ea) je mera nastajanja rekombinacionih reakcija povezanih sa uspostavljanjem ZF hlorofila, a time i ukupnih promena u strukturi i funkciji tilakodinih membrana. Ostvareni rezultati i diskusija izloženih parametara ukupnih fotosintetično-termalnih procesa ZF hlorofila mogu doprineti egzaktnijem karakterisanju ispitivanih samooplodnih linija kukuruza u odnosu na njihovu plastičnost, tolerantnost, otpornost i adaptivnost prema temperaturi i suši, što samim tim i doprinosi bržem i racionalnijem unapređenju procesa selekcije i produktivne semenske proizvodnje.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - The improved photosynthetic-fluorescence method and its application in breeding and seed production of maize
T1  - Poboljšani fotositetično-fluorescentni metod i njegova primena u oplemenjivanju i semenarstvu kukuruza
VL  - 68
IS  - 3
SP  - 15
EP  - 30
ER  - 
@article{
author = "Radenović, Čedomir and Saratlić, Goran and Anđelković, Violeta and Radojčić, Aleksandar and Selaković, Dragojlo and Hojka, Zdravko",
year = "2007",
url = "http://rik.mrizp.rs/handle/123456789/166",
abstract = "The application of the improved non-invasive photosynthetic-fluorescence method in maize breeding and seed production was observed in order to assess the resistance and adaptability of maize inbred lines to higher and high temperatures, as well as to drought. The studies were carried out with two maize inbred lines (ZPPL 115 and A 671). The thermal characteristics of the photosynthetic apparatus of the investigated maize inbred lines were determined as follows: temperature dependency range from 25 to 60oC, critical temperatures at which phase transitions take place in the thylakoid membrane, causing significant structural and functional changes in the photosynthetic apparatus of the investigated inbred lines, activation energies (Ea, kJ/mol) were calculated along the straight lines before and after the occurrence of the critical temperature. The activation energy (Ea) is a measure of the intensity of recombining responses to chlorophyll DF, and total changes in the structure and functioning of the thylakoid membranes. The results obtained and presented parameters of total photosynthetic and thermal processes of chlorophyll DF may contribute to a more accurate characterisation of maize inbred lines in respect to their resistance and adaptability to temperatures and drought. Consecutively, this can lead to a quicker and more rational improvement maize breeding and seed production., Poboljšani neinvazivni fotosintetično-fluorescentni metod kandiduje se za primenu u oplemenjivanju i semenarstvu radi utvrđivanja ocene samooplodnih linija kukuruza na plastičnost, tolerantnost, otpornost i adaptivnost prema višim i visokim temperaturama, kao i prema suši. Utvrđene su termalne karakteristike fotosintetičnog aparata proučavanih samooplodnih linija kukuruza, i to: određena je temperaturna zavisnost u opsegu od 25 do 60oC, otkrivene su kritične temperature na kojima dolazi do faznih transformacija u tilakoidnoj membrani, a time i do značajnih strukturnih i funkcionalnih promena u fotosintetičnom aparatu ispitivanih samooplodnih linija kukuruza, obračunate su energije aktivacije (Ea, kJ/mol) duž pravih linija pre i posle pojavljivanja kritične temperature. Energija aktivacije (Ea) je mera nastajanja rekombinacionih reakcija povezanih sa uspostavljanjem ZF hlorofila, a time i ukupnih promena u strukturi i funkciji tilakodinih membrana. Ostvareni rezultati i diskusija izloženih parametara ukupnih fotosintetično-termalnih procesa ZF hlorofila mogu doprineti egzaktnijem karakterisanju ispitivanih samooplodnih linija kukuruza u odnosu na njihovu plastičnost, tolerantnost, otpornost i adaptivnost prema temperaturi i suši, što samim tim i doprinosi bržem i racionalnijem unapređenju procesa selekcije i produktivne semenske proizvodnje.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "The improved photosynthetic-fluorescence method and its application in breeding and seed production of maize, Poboljšani fotositetično-fluorescentni metod i njegova primena u oplemenjivanju i semenarstvu kukuruza",
volume = "68",
number = "3",
pages = "15-30"
}
Radenović, Č., Saratlić, G., Anđelković, V., Radojčić, A., Selaković, D.,& Hojka, Z. (2007). Poboljšani fotositetično-fluorescentni metod i njegova primena u oplemenjivanju i semenarstvu kukuruza.
Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 68(3), 15-30.
Radenović Č, Saratlić G, Anđelković V, Radojčić A, Selaković D, Hojka Z. Poboljšani fotositetično-fluorescentni metod i njegova primena u oplemenjivanju i semenarstvu kukuruza. Journal of Scientific Agricultural Research. 2007;68(3):15-30
Radenović Čedomir, Saratlić Goran, Anđelković Violeta, Radojčić Aleksandar, Selaković Dragojlo, Hojka Zdravko, "Poboljšani fotositetično-fluorescentni metod i njegova primena u oplemenjivanju i semenarstvu kukuruza" Journal of Scientific Agricultural Research, 68, no. 3 (2007):15-30

The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations

Marković, Ksenija; Ignjatović-Micić, Dragana; Saratlić, Goran; Lazić-Jančić, Vesna

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2007)

TY  - JOUR
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Saratlić, Goran
AU  - Lazić-Jančić, Vesna
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/168
AB  - RFLP loci, potential indicators of kernel high oil content, were identified using bulk segregant analysis (BSA) approach, i.e. bulked samples derived from C0 and C9 selection cycles of two maize synthetic populations - DS7u and YuSSSu. The kernel oil content was determined by the NMR spectroscopy. The genome analysis of bulked samples was carried out with 57 RFLP markers (UMC core set). On the basis of allele frequency differences between cycles of selection, informative RFLP markers (potentially closely linked to QTLs regulating high oil content in maize) were identified. Four common RFLP loci were identified in both DS7u and YuSSSu populations. Additional four RFLPs were identified in DS7u population.
AB  - RFLP lokusi, potencijalni indikatori povećanog sadržaja ulja u zrnu, identifikovani su analizom grupnih uzoraka (Bulk Segregant Analysis - BSA) C0 i C9 ciklusa selekcije dve domaće sintetičke populacije kukuruza - DS7u i YuSSSu. Sadržaj ulja u zrnu biljaka ispitivanih ciklusa selekcije obe populacije utvrđen je NMR spektroskopijom. Analiza genoma grupnih uzoraka početnog (C0) i krajnjeg (C9) ciklusa selekcije DS7u i YuSSSu urađena je pomoću 57 RFLP markera UMC core set. Na osnovu promena u frekvenciji alela u grupnim uzorcima u toku procesa selekcije analiziranih populacija utvrđene su informativne probe - probe potencijalno blisko vezane za QTL uključene u kontrolu povećanog sadržaja ulja u zrnu kukuruza. Identifikovana su četiri zajednička RFLP lokusa u obe analizirane populacije, s tim što je u populaciji DS7u identifikovano još četiri RFLP lokusa potencijalnih indikatora povećanog sadržaja ulja u zrnu kukuruza.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations
T1  - Primena molekularnih markera u analizi sadržaja ulja u zrnu grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L.)
VL  - 68
IS  - 2
SP  - 5
EP  - 15
ER  - 
@article{
author = "Marković, Ksenija and Ignjatović-Micić, Dragana and Saratlić, Goran and Lazić-Jančić, Vesna",
year = "2007",
url = "http://rik.mrizp.rs/handle/123456789/168",
abstract = "RFLP loci, potential indicators of kernel high oil content, were identified using bulk segregant analysis (BSA) approach, i.e. bulked samples derived from C0 and C9 selection cycles of two maize synthetic populations - DS7u and YuSSSu. The kernel oil content was determined by the NMR spectroscopy. The genome analysis of bulked samples was carried out with 57 RFLP markers (UMC core set). On the basis of allele frequency differences between cycles of selection, informative RFLP markers (potentially closely linked to QTLs regulating high oil content in maize) were identified. Four common RFLP loci were identified in both DS7u and YuSSSu populations. Additional four RFLPs were identified in DS7u population., RFLP lokusi, potencijalni indikatori povećanog sadržaja ulja u zrnu, identifikovani su analizom grupnih uzoraka (Bulk Segregant Analysis - BSA) C0 i C9 ciklusa selekcije dve domaće sintetičke populacije kukuruza - DS7u i YuSSSu. Sadržaj ulja u zrnu biljaka ispitivanih ciklusa selekcije obe populacije utvrđen je NMR spektroskopijom. Analiza genoma grupnih uzoraka početnog (C0) i krajnjeg (C9) ciklusa selekcije DS7u i YuSSSu urađena je pomoću 57 RFLP markera UMC core set. Na osnovu promena u frekvenciji alela u grupnim uzorcima u toku procesa selekcije analiziranih populacija utvrđene su informativne probe - probe potencijalno blisko vezane za QTL uključene u kontrolu povećanog sadržaja ulja u zrnu kukuruza. Identifikovana su četiri zajednička RFLP lokusa u obe analizirane populacije, s tim što je u populaciji DS7u identifikovano još četiri RFLP lokusa potencijalnih indikatora povećanog sadržaja ulja u zrnu kukuruza.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations, Primena molekularnih markera u analizi sadržaja ulja u zrnu grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L.)",
volume = "68",
number = "2",
pages = "5-15"
}
Marković, K., Ignjatović-Micić, D., Saratlić, G.,& Lazić-Jančić, V. (2007). Primena molekularnih markera u analizi sadržaja ulja u zrnu grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L.).
Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 68(2), 5-15.
Marković K, Ignjatović-Micić D, Saratlić G, Lazić-Jančić V. Primena molekularnih markera u analizi sadržaja ulja u zrnu grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L.). Journal of Scientific Agricultural Research. 2007;68(2):5-15
Marković Ksenija, Ignjatović-Micić Dragana, Saratlić Goran, Lazić-Jančić Vesna, "Primena molekularnih markera u analizi sadržaja ulja u zrnu grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L.)" Journal of Scientific Agricultural Research, 68, no. 2 (2007):5-15

Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations

Marković, Ksenija; Ignjatović-Micić, Dragana; Saratlić, Goran; Lazić-Jančić, Vesna

(Društvo genetičara Srbije, Beograd, 2007)

TY  - JOUR
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Saratlić, Goran
AU  - Lazić-Jančić, Vesna
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/151
AB  - Chromosome regions determining kernel high oil content were identified by RFLP analysis of individual plants from C0 and C9 selection cycles of two maize synthetic populations -DS7u and YuSSSu. Identification of chromosome regions was done with informative RFLP markers (that were identified with BSA earlier) using a single plant approach - analysis of individual plants. This analysis revealed the number of plants carrying alleles that endured frequency alterations during selection process. Statistical analysis (χ2 test) revealed chromosome regions that comprise putative QTLs affecting expression of kernel high oil content of analyzed maize populations. Four regions on chromosomes 1, 6, 7 and 8 were identified in both DS7u and YuSSSu populations. Additional four regions on chromosome 4, 9 and 10 were detected only in DS7u population.
AB  - Hromozomski regioni uključeni u ekspresiju visokog sadržaja ulja u zrnu kukuruza su identifikovani RFLP analizom individualnih biljaka C0 i C9 ciklusa selekcije dve sintetičke populacije kukuruza -DS7u i YuSSSu. Identifikacija hromozomskih regiona je rađena analizom pojedinačnih biljaka iz grupnih uzoraka sa informativnim RFLP markerima (koji su otkriveni analizom grupnih uzoraka u prvom delu eksperimenta). Rezultati ove analize su ukazali na broj biljaka nosioca alela kod kojih je došlo do promena u frekvenciji tokom selekcije. Statističkom analizom (χ2 test) rezultata identifikovani su hromozomski regioni nosioci potencijalnih QTL koji utiču na ekspresiju visokog sadržaja ulja u zrnu analiziranih populacija. Četiri zajednička regiona je identifikovano u obe analizirane populacije, na hromozomima 1, 6, 7 i 8. U populaciji DS7u je identifikovano još četiri regiona, na hromozomima 4, 9 i 10.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations
T1  - Identifikacija hromozomskih regiona uključenih u kontrolu visokog sadržaja ulja u zrnu sintetičkih populacija kukuruza (Zea mays L.)
VL  - 39
IS  - 2
SP  - 197
EP  - 206
DO  - 10.2298/GENSR0702197M
ER  - 
@article{
author = "Marković, Ksenija and Ignjatović-Micić, Dragana and Saratlić, Goran and Lazić-Jančić, Vesna",
year = "2007",
url = "http://rik.mrizp.rs/handle/123456789/151",
abstract = "Chromosome regions determining kernel high oil content were identified by RFLP analysis of individual plants from C0 and C9 selection cycles of two maize synthetic populations -DS7u and YuSSSu. Identification of chromosome regions was done with informative RFLP markers (that were identified with BSA earlier) using a single plant approach - analysis of individual plants. This analysis revealed the number of plants carrying alleles that endured frequency alterations during selection process. Statistical analysis (χ2 test) revealed chromosome regions that comprise putative QTLs affecting expression of kernel high oil content of analyzed maize populations. Four regions on chromosomes 1, 6, 7 and 8 were identified in both DS7u and YuSSSu populations. Additional four regions on chromosome 4, 9 and 10 were detected only in DS7u population., Hromozomski regioni uključeni u ekspresiju visokog sadržaja ulja u zrnu kukuruza su identifikovani RFLP analizom individualnih biljaka C0 i C9 ciklusa selekcije dve sintetičke populacije kukuruza -DS7u i YuSSSu. Identifikacija hromozomskih regiona je rađena analizom pojedinačnih biljaka iz grupnih uzoraka sa informativnim RFLP markerima (koji su otkriveni analizom grupnih uzoraka u prvom delu eksperimenta). Rezultati ove analize su ukazali na broj biljaka nosioca alela kod kojih je došlo do promena u frekvenciji tokom selekcije. Statističkom analizom (χ2 test) rezultata identifikovani su hromozomski regioni nosioci potencijalnih QTL koji utiču na ekspresiju visokog sadržaja ulja u zrnu analiziranih populacija. Četiri zajednička regiona je identifikovano u obe analizirane populacije, na hromozomima 1, 6, 7 i 8. U populaciji DS7u je identifikovano još četiri regiona, na hromozomima 4, 9 i 10.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations, Identifikacija hromozomskih regiona uključenih u kontrolu visokog sadržaja ulja u zrnu sintetičkih populacija kukuruza (Zea mays L.)",
volume = "39",
number = "2",
pages = "197-206",
doi = "10.2298/GENSR0702197M"
}
Marković, K., Ignjatović-Micić, D., Saratlić, G.,& Lazić-Jančić, V. (2007). Identifikacija hromozomskih regiona uključenih u kontrolu visokog sadržaja ulja u zrnu sintetičkih populacija kukuruza (Zea mays L.).
Genetika
Društvo genetičara Srbije, Beograd., 39(2), 197-206.
https://doi.org/10.2298/GENSR0702197M
Marković K, Ignjatović-Micić D, Saratlić G, Lazić-Jančić V. Identifikacija hromozomskih regiona uključenih u kontrolu visokog sadržaja ulja u zrnu sintetičkih populacija kukuruza (Zea mays L.). Genetika. 2007;39(2):197-206
Marković Ksenija, Ignjatović-Micić Dragana, Saratlić Goran, Lazić-Jančić Vesna, "Identifikacija hromozomskih regiona uključenih u kontrolu visokog sadržaja ulja u zrnu sintetičkih populacija kukuruza (Zea mays L.)" Genetika, 39, no. 2 (2007):197-206,
https://doi.org/10.2298/GENSR0702197M .
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