Stanković, Goran

Link to this page

Authority KeyName Variants
orcid::0000-0002-7723-3238
  • Stanković, Goran (33)
Projects

Author's Bibliography

First report of Aspergillus parasiticus on Barley Grain in Serbia

Nikolić, Milica; Savić, Iva; Obradović, Ana; Srdić, Jelena; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(St. Paul : The American Phytopathological Society (APS), 2020)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Obradović, Ana
AU  - Srdić, Jelena
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/796
AB  - Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.
PB  - St. Paul : The American Phytopathological Society (APS)
T2  - Plant Disease
T1  - First report of Aspergillus parasiticus on Barley Grain in Serbia
VL  - 104
IS  - 3
SP  - 987
DO  - 10.1094/PDIS-07-19-1364-PDN
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Obradović, Ana and Srdić, Jelena and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2020",
abstract = "Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.",
publisher = "St. Paul : The American Phytopathological Society (APS)",
journal = "Plant Disease",
title = "First report of Aspergillus parasiticus on Barley Grain in Serbia",
volume = "104",
number = "3",
pages = "987",
doi = "10.1094/PDIS-07-19-1364-PDN"
}
Nikolić, M., Savić, I., Obradović, A., Srdić, J., Stanković, G., Stevanović, M.,& Stanković, S.. (2020). First report of Aspergillus parasiticus on Barley Grain in Serbia. in Plant Disease
St. Paul : The American Phytopathological Society (APS)., 104(3), 987.
https://doi.org/10.1094/PDIS-07-19-1364-PDN
Nikolić M, Savić I, Obradović A, Srdić J, Stanković G, Stevanović M, Stanković S. First report of Aspergillus parasiticus on Barley Grain in Serbia. in Plant Disease. 2020;104(3):987.
doi:10.1094/PDIS-07-19-1364-PDN .
Nikolić, Milica, Savić, Iva, Obradović, Ana, Srdić, Jelena, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First report of Aspergillus parasiticus on Barley Grain in Serbia" in Plant Disease, 104, no. 3 (2020):987,
https://doi.org/10.1094/PDIS-07-19-1364-PDN . .

Assessment stability of maize lines yield by GGE-biplot analysis

Božović, Dragan; Zivanović, Tomislav; Popović, Vera; Tatić, Mladen; Gospavić, Zagorka; Miloradović, Zoran; Dokić, Milorad; Stanković, Goran

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Božović, Dragan
AU  - Zivanović, Tomislav
AU  - Popović, Vera
AU  - Tatić, Mladen
AU  - Gospavić, Zagorka
AU  - Miloradović, Zoran
AU  - Dokić, Milorad
AU  - Stanković, Goran
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/706
AB  - Maize genotypes have varied reactions in different localities, years, treatments or the combination of these factors, due to genotype x year interaction. The objective of this study was to estimate genotype by locality, by year, by treatments (GxLxYxT) interaction using AMMI model, to identify maize genotypes with stable and high yield performance in different growing seasons. The trials with seven maize lines/genotypes were conducted during two years (2010-2011) at the four treatments and two locations: Pancevo and Zemun Polje. The results showed that the influence of: genotype (G), locality (L), treatment (T) and GxL, GxT, YxL, YxT, LXT, GxYxT, GxLxT, YxLxT, GxYxLxT interaction, on maize yield were significant (p lt 0.01). The share of genotype for maize grain yield in the total phenotypic variance was 21.16%, the aggregate share of the years and the locality was 6.10%, the treatment was 18.22%, and the total interaction was 54.52%. The AMMI analysis of the main components of IPCA1 and IPCA2 for the interaction of GxL and GxT shows that the first major component, IPCA1, comprises 100% of the sum of the squared interaction GxL and showed a statistically significant effect. The results also show that the sums of the squares of the first and second major components (PC1 and PC2) constitute 100% of the sum of the squared interaction GxL. The IPCA1 share in the GxT interaction was 47.39% and the IPC2 was 37.94%. IPC1 and IPC2 for this interaction was 85.33%. A high level of IPC2 indicates a significant treatment effect. The results of AMI analysis show that there is a significant difference between the genotype reactions to different ecological conditions for investigated factors. It also provided better insight in specific association between maize grain yield, locality, treatment and meteorological variables. Among the tested maize lines/genotypes, L-5, L-4 and L-6 could be separated as highest yielding genotypes, however L-5 could be recommended for further breeding program and in large-scale seed production due to its stable and high yielding performance.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Assessment stability of maize lines yield by GGE-biplot analysis
VL  - 50
IS  - 3
SP  - 755
EP  - 770
DO  - 10.2298/GENSR1803755B
UR  - conv_1001
ER  - 
@article{
author = "Božović, Dragan and Zivanović, Tomislav and Popović, Vera and Tatić, Mladen and Gospavić, Zagorka and Miloradović, Zoran and Dokić, Milorad and Stanković, Goran",
year = "2018",
abstract = "Maize genotypes have varied reactions in different localities, years, treatments or the combination of these factors, due to genotype x year interaction. The objective of this study was to estimate genotype by locality, by year, by treatments (GxLxYxT) interaction using AMMI model, to identify maize genotypes with stable and high yield performance in different growing seasons. The trials with seven maize lines/genotypes were conducted during two years (2010-2011) at the four treatments and two locations: Pancevo and Zemun Polje. The results showed that the influence of: genotype (G), locality (L), treatment (T) and GxL, GxT, YxL, YxT, LXT, GxYxT, GxLxT, YxLxT, GxYxLxT interaction, on maize yield were significant (p lt 0.01). The share of genotype for maize grain yield in the total phenotypic variance was 21.16%, the aggregate share of the years and the locality was 6.10%, the treatment was 18.22%, and the total interaction was 54.52%. The AMMI analysis of the main components of IPCA1 and IPCA2 for the interaction of GxL and GxT shows that the first major component, IPCA1, comprises 100% of the sum of the squared interaction GxL and showed a statistically significant effect. The results also show that the sums of the squares of the first and second major components (PC1 and PC2) constitute 100% of the sum of the squared interaction GxL. The IPCA1 share in the GxT interaction was 47.39% and the IPC2 was 37.94%. IPC1 and IPC2 for this interaction was 85.33%. A high level of IPC2 indicates a significant treatment effect. The results of AMI analysis show that there is a significant difference between the genotype reactions to different ecological conditions for investigated factors. It also provided better insight in specific association between maize grain yield, locality, treatment and meteorological variables. Among the tested maize lines/genotypes, L-5, L-4 and L-6 could be separated as highest yielding genotypes, however L-5 could be recommended for further breeding program and in large-scale seed production due to its stable and high yielding performance.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Assessment stability of maize lines yield by GGE-biplot analysis",
volume = "50",
number = "3",
pages = "755-770",
doi = "10.2298/GENSR1803755B",
url = "conv_1001"
}
Božović, D., Zivanović, T., Popović, V., Tatić, M., Gospavić, Z., Miloradović, Z., Dokić, M.,& Stanković, G.. (2018). Assessment stability of maize lines yield by GGE-biplot analysis. in Genetika
Društvo genetičara Srbije, Beograd., 50(3), 755-770.
https://doi.org/10.2298/GENSR1803755B
conv_1001
Božović D, Zivanović T, Popović V, Tatić M, Gospavić Z, Miloradović Z, Dokić M, Stanković G. Assessment stability of maize lines yield by GGE-biplot analysis. in Genetika. 2018;50(3):755-770.
doi:10.2298/GENSR1803755B
conv_1001 .
Božović, Dragan, Zivanović, Tomislav, Popović, Vera, Tatić, Mladen, Gospavić, Zagorka, Miloradović, Zoran, Dokić, Milorad, Stanković, Goran, "Assessment stability of maize lines yield by GGE-biplot analysis" in Genetika, 50, no. 3 (2018):755-770,
https://doi.org/10.2298/GENSR1803755B .,
conv_1001 .
6
4
8

Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence

Obradović, Ana; Krnjaja, Vesna; Nikolić, Milica; Delibašić, Goran; Filipović, Milomir; Stanković, Goran; Stanković, Slavica

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Nikolić, Milica
AU  - Delibašić, Goran
AU  - Filipović, Milomir
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/738
AB  - Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species.
AB  - Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence
T1  - Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava
VL  - 34
IS  - 4
SP  - 469
EP  - 480
DO  - 10.2298/BAH1804469O
UR  - conv_2004
ER  - 
@article{
author = "Obradović, Ana and Krnjaja, Vesna and Nikolić, Milica and Delibašić, Goran and Filipović, Milomir and Stanković, Goran and Stanković, Slavica",
year = "2018",
abstract = "Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species., Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence, Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava",
volume = "34",
number = "4",
pages = "469-480",
doi = "10.2298/BAH1804469O",
url = "conv_2004"
}
Obradović, A., Krnjaja, V., Nikolić, M., Delibašić, G., Filipović, M., Stanković, G.,& Stanković, S.. (2018). Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(4), 469-480.
https://doi.org/10.2298/BAH1804469O
conv_2004
Obradović A, Krnjaja V, Nikolić M, Delibašić G, Filipović M, Stanković G, Stanković S. Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence. in Biotechnology in Animal Husbandry. 2018;34(4):469-480.
doi:10.2298/BAH1804469O
conv_2004 .
Obradović, Ana, Krnjaja, Vesna, Nikolić, Milica, Delibašić, Goran, Filipović, Milomir, Stanković, Goran, Stanković, Slavica, "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence" in Biotechnology in Animal Husbandry, 34, no. 4 (2018):469-480,
https://doi.org/10.2298/BAH1804469O .,
conv_2004 .
4

Comparison of statistical methods for genetic similarity evaluation of maize inbred lines

Nikolić, Ana; Kostadinović, Marija; Vančetović, Jelena; Stanković, Goran; Ignjatović-Micić, Dragana

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2017)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Ignjatović-Micić, Dragana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/679
AB  - Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index.
AB  - Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Comparison of statistical methods for genetic similarity evaluation of maize inbred lines
T1  - Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza
VL  - 54
IS  - 1
SP  - 25
EP  - 30
DO  - 10.5937/ratpov54-12174
UR  - conv_623
ER  - 
@article{
author = "Nikolić, Ana and Kostadinović, Marija and Vančetović, Jelena and Stanković, Goran and Ignjatović-Micić, Dragana",
year = "2017",
abstract = "Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index., Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines, Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza",
volume = "54",
number = "1",
pages = "25-30",
doi = "10.5937/ratpov54-12174",
url = "conv_623"
}
Nikolić, A., Kostadinović, M., Vančetović, J., Stanković, G.,& Ignjatović-Micić, D.. (2017). Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 54(1), 25-30.
https://doi.org/10.5937/ratpov54-12174
conv_623
Nikolić A, Kostadinović M, Vančetović J, Stanković G, Ignjatović-Micić D. Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo. 2017;54(1):25-30.
doi:10.5937/ratpov54-12174
conv_623 .
Nikolić, Ana, Kostadinović, Marija, Vančetović, Jelena, Stanković, Goran, Ignjatović-Micić, Dragana, "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines" in Ratarstvo i povrtarstvo, 54, no. 1 (2017):25-30,
https://doi.org/10.5937/ratpov54-12174 .,
conv_623 .
1

Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits

Kostadinović, Marija; Ignjatović-Micić, Dragana; Ristić, Danijela; Mladenović-Drinić, Snežana; Vančetović, Jelena; Božinović, Sofija; Stanković, Goran

(Public Library Science, San Francisco, 2016)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Stanković, Goran
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/621
AB  - Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.
PB  - Public Library Science, San Francisco
T2  - PLOS ONE
T1  - Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167635
UR  - conv_941
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Ristić, Danijela and Mladenović-Drinić, Snežana and Vančetović, Jelena and Božinović, Sofija and Stanković, Goran",
year = "2016",
abstract = "Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.",
publisher = "Public Library Science, San Francisco",
journal = "PLOS ONE",
title = "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167635",
url = "conv_941"
}
Kostadinović, M., Ignjatović-Micić, D., Ristić, D., Mladenović-Drinić, S., Vančetović, J., Božinović, S.,& Stanković, G.. (2016). Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167635
conv_941
Kostadinović M, Ignjatović-Micić D, Ristić D, Mladenović-Drinić S, Vančetović J, Božinović S, Stanković G. Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE. 2016;11(12).
doi:10.1371/journal.pone.0167635
conv_941 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Ristić, Danijela, Mladenović-Drinić, Snežana, Vančetović, Jelena, Božinović, Sofija, Stanković, Goran, "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits" in PLOS ONE, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167635 .,
conv_941 .
1
7
7
7

Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima

Stanković, Goran

(Универзитет у Београду, Пољопривредни факултет, 2016)

TY  - THES
AU  - Stanković, Goran
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=3772
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:12683/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48149519
UR  - http://nardus.mpn.gov.rs/123456789/6426
UR  - http://rik.mrizp.rs/handle/123456789/761
AB  - U radu je proučavana divergentnost sintetičkih populacija, roditeljskih linija i linija potomstava analizom srednjih vrednosti njihovih deset osobina i primenom SSR markera. Za istraţivanja su korišćene tri sintetičke populacije per se i njihove S1 familije, deset roditeljskih linija i jedanaest linija potomstava. Sintetička populacija Syn ZP je nastala dialelnim ukrštanjem devet inbred linija poreklom iz lokalnih sorti sa Zapadnog Balkana, Syn ZP/USA iz ukrštanja linija lokalnog porekla sa linijom poreklom iz BSS sintetika, a Syn FS rekombinacijom predhodne dve populacije. Linije potomstva su poreklom iz sintetičkih populacija Syn ZP i Syn ZP/USA.Na osnovu rezultata istraţivanja utvrĎeno je da su se S1 familije tri sintetičke populacije značajno razlikovale po srednjim vrednostima ispitivanih osobina. Varijabilnost i heritabilnost ispitivanih osobina je povećana nakon unošenja strane germplazme, kao i nakon rekombinacije sintetičkih populacija, a naročito je izraţeno povećanje genetičke dobiti od selekcije. Genetički koeficijenti korelacija su pokazali da je kod sve tri populacije najveći uticaj na prinos zrna imala duţina klipa, a najmanji masa 1000 zrna. Rezultati analize glavnih komponenti su pokazali da je duţina klipa, zajedno sa brojem zrna u redu, imala najveći indirektan uticaj na prinos zrna kod S1 familija sve tri sintetičke populacije. Inkorporacija strane germplazme, kao i rekombinacija sintetičkih populacija, su doprinele stvaranju nove varijabilnosti ispitivanog genetičkog materijala i poboljšanju srednjih vrednosti ispitivanih osobina.Rezultati istraţivanja sintetičkih populacija per se, roditeljskih komponenti i linija potomstava su ukazali da su selekcionisane linije imale bolje srednje vrednosti za ispitivane osobine od roditeljskih linija. Koeficijenti korelacija su bili pribliţno isti kod linija roditelja i linija potomstava, s tim da je uočeno povećanje uticaja mase zrna naprinos, u odnosu na S1 familije. Na osnovu ovih rezultata odabrano je nekoliko linija potomstava kod kojih je zabeleţeno poboljšanje osobina u odnosu na roditelje i koje mogu biti perspektivne za dalju selekciju...
AB  - Divergence of synthetic populations, parental and progeny inbred lines has been studied using both mean values of ten phenotypic traits and SSR markers. The research was performed on three synthetic populations per se and their S1 families, ten parental and 11 progeny inbred lines. Syn ZP synthetic population was obtained by diallel crossing of nine inbreds that originated from local, West Balkans, landraces, Syn ZP/USA by crossing inbreds of local origin with an inbred from BSS synthetic and Syn FS by recombination of the two previous populations. Progeny inbred lines were obtained from Syn ZP and Syn ZP/USA.The results revealed that S1 families of the three synthetics were significantly different in the mean values of the analysed traits. Variability and heritability of these traits has been increased after introduction of exotic germplasm as well as after recombination of the synthetic populations and genetic gain was particularly expressed. Genetic correlation coefficients showed that ear length had the highest and 1000 kernel weight the lowest impact on grain yield. Principal component analysis showed that ear length, together with kernel number per row, had the highest indirect impact on grain yield of S1 families considering all three synthetic populations. Incorporation of exotic germplasm, as well as recombination of the synthetic populations, contributed to the generation of new variability in the genetic material and improvement of mean values of the analysed traits.Analyses of synthetic populations per se, parental components and progeny inbreds indicated that new inbred lines had better mean values of the traits compared to parental inbreds. Correlation coefficients were approximately the same within parental inbreds and within progeny inbreds, except that increase of kernel weight impact ongrain yield in regard to S1 families was observed. Based on the presented results of the research, several progeny inbreds have been chosen, which have better trait performances in comparison to parents and could be perspective for further selection...
PB  - Универзитет у Београду, Пољопривредни факултет
T2  - Универзитет у Београду
T1  - Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima
T1  - Divergence of maize synthetic populations and their progenies determined by phenotypic and molecular markers
ER  - 
@phdthesis{
author = "Stanković, Goran",
year = "2016",
abstract = "U radu je proučavana divergentnost sintetičkih populacija, roditeljskih linija i linija potomstava analizom srednjih vrednosti njihovih deset osobina i primenom SSR markera. Za istraţivanja su korišćene tri sintetičke populacije per se i njihove S1 familije, deset roditeljskih linija i jedanaest linija potomstava. Sintetička populacija Syn ZP je nastala dialelnim ukrštanjem devet inbred linija poreklom iz lokalnih sorti sa Zapadnog Balkana, Syn ZP/USA iz ukrštanja linija lokalnog porekla sa linijom poreklom iz BSS sintetika, a Syn FS rekombinacijom predhodne dve populacije. Linije potomstva su poreklom iz sintetičkih populacija Syn ZP i Syn ZP/USA.Na osnovu rezultata istraţivanja utvrĎeno je da su se S1 familije tri sintetičke populacije značajno razlikovale po srednjim vrednostima ispitivanih osobina. Varijabilnost i heritabilnost ispitivanih osobina je povećana nakon unošenja strane germplazme, kao i nakon rekombinacije sintetičkih populacija, a naročito je izraţeno povećanje genetičke dobiti od selekcije. Genetički koeficijenti korelacija su pokazali da je kod sve tri populacije najveći uticaj na prinos zrna imala duţina klipa, a najmanji masa 1000 zrna. Rezultati analize glavnih komponenti su pokazali da je duţina klipa, zajedno sa brojem zrna u redu, imala najveći indirektan uticaj na prinos zrna kod S1 familija sve tri sintetičke populacije. Inkorporacija strane germplazme, kao i rekombinacija sintetičkih populacija, su doprinele stvaranju nove varijabilnosti ispitivanog genetičkog materijala i poboljšanju srednjih vrednosti ispitivanih osobina.Rezultati istraţivanja sintetičkih populacija per se, roditeljskih komponenti i linija potomstava su ukazali da su selekcionisane linije imale bolje srednje vrednosti za ispitivane osobine od roditeljskih linija. Koeficijenti korelacija su bili pribliţno isti kod linija roditelja i linija potomstava, s tim da je uočeno povećanje uticaja mase zrna naprinos, u odnosu na S1 familije. Na osnovu ovih rezultata odabrano je nekoliko linija potomstava kod kojih je zabeleţeno poboljšanje osobina u odnosu na roditelje i koje mogu biti perspektivne za dalju selekciju..., Divergence of synthetic populations, parental and progeny inbred lines has been studied using both mean values of ten phenotypic traits and SSR markers. The research was performed on three synthetic populations per se and their S1 families, ten parental and 11 progeny inbred lines. Syn ZP synthetic population was obtained by diallel crossing of nine inbreds that originated from local, West Balkans, landraces, Syn ZP/USA by crossing inbreds of local origin with an inbred from BSS synthetic and Syn FS by recombination of the two previous populations. Progeny inbred lines were obtained from Syn ZP and Syn ZP/USA.The results revealed that S1 families of the three synthetics were significantly different in the mean values of the analysed traits. Variability and heritability of these traits has been increased after introduction of exotic germplasm as well as after recombination of the synthetic populations and genetic gain was particularly expressed. Genetic correlation coefficients showed that ear length had the highest and 1000 kernel weight the lowest impact on grain yield. Principal component analysis showed that ear length, together with kernel number per row, had the highest indirect impact on grain yield of S1 families considering all three synthetic populations. Incorporation of exotic germplasm, as well as recombination of the synthetic populations, contributed to the generation of new variability in the genetic material and improvement of mean values of the analysed traits.Analyses of synthetic populations per se, parental components and progeny inbreds indicated that new inbred lines had better mean values of the traits compared to parental inbreds. Correlation coefficients were approximately the same within parental inbreds and within progeny inbreds, except that increase of kernel weight impact ongrain yield in regard to S1 families was observed. Based on the presented results of the research, several progeny inbreds have been chosen, which have better trait performances in comparison to parents and could be perspective for further selection...",
publisher = "Универзитет у Београду, Пољопривредни факултет",
journal = "Универзитет у Београду",
title = "Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima, Divergence of maize synthetic populations and their progenies determined by phenotypic and molecular markers"
}
Stanković, G.. (2016). Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima. in Универзитет у Београду
Универзитет у Београду, Пољопривредни факултет..
Stanković G. Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima. in Универзитет у Београду. 2016;..
Stanković, Goran, "Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima" in Универзитет у Београду (2016).

Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value

Radenović, Čedomir; Delić, Nenad; Sečanski, Mile; Jovanović, Života; Stanković, Goran; Popović, Aleksandar

(2015)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Delić, Nenad
AU  - Sečanski, Mile
AU  - Jovanović, Života
AU  - Stanković, Goran
AU  - Popović, Aleksandar
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/601
AB  - This paper presents results of several different studies that confirm the hypothesis that maize inbred lines rich in proteins and with exceptional nutritive values can be bred. This is also supported by a medicinal standpoint of programmed need for maize-based food and feed. With such an experimental approach, the maize inbred lines ZPPL 146 and ZPPL 159 and hybrids derived from them (ZP 633, ZP 735 and ZP 737) rich in proteins have been systematically tested. Based on concrete results the following we can be conclude as follows. Selected maize inbred lines ZPPL 146 and ZPPL 159, rich in carotenoids, yellow pigments, also have significant amounts of other relevant bioactive compounds. Observed inbred lines have erect top leaves and are classified into a group of maize inbreds with significant properties of the photosynthesis model, are resistant to high temperatures and tolerant to drought. Spectral bands pointing to conformational characteristics of molecules of carotenoids but also of other compounds (phosphates, glutens and amides III) were established by the resonance Raman spectroscopy method applied to the leaf of the maize inbred line ZPLL 146. Physiological, biochemical and biophysical traits of elite maize inbred lines and their hybrids were observed in this study with a special emphasis on their efficiency of photosynthesis, productivity and suitability for broad use of nutrient values of grain and other essential biogenic matters, first of all pigments that express antioxidative properties. Relevant traits, properties and parameters of observed maize inbred lines that can be used in the process of selection are presented. These prestigious maize inbred lines were used to develop high-yielding and high-quality maize hybrids (ZP 633, ZP 735 and ZP 737) that are recognisable for their quality in human nutrition (children and the elderly), that are confirmed by medical observations related to their use in food and feed. Relevant agronomic and morphological traits of maize hybrids are presented. Moreover, results on grain structure and yields obtained in the regions of south-eastern Europe are also displayed.
T2  - Sel'skokhozyaistvennaya Biologiya
T1  - Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value
VL  - 50
IS  - 5
SP  - 600
EP  - 610
DO  - 10.15389/agrobiology.2015.5.600eng
UR  - conv_1022
ER  - 
@article{
author = "Radenović, Čedomir and Delić, Nenad and Sečanski, Mile and Jovanović, Života and Stanković, Goran and Popović, Aleksandar",
year = "2015",
abstract = "This paper presents results of several different studies that confirm the hypothesis that maize inbred lines rich in proteins and with exceptional nutritive values can be bred. This is also supported by a medicinal standpoint of programmed need for maize-based food and feed. With such an experimental approach, the maize inbred lines ZPPL 146 and ZPPL 159 and hybrids derived from them (ZP 633, ZP 735 and ZP 737) rich in proteins have been systematically tested. Based on concrete results the following we can be conclude as follows. Selected maize inbred lines ZPPL 146 and ZPPL 159, rich in carotenoids, yellow pigments, also have significant amounts of other relevant bioactive compounds. Observed inbred lines have erect top leaves and are classified into a group of maize inbreds with significant properties of the photosynthesis model, are resistant to high temperatures and tolerant to drought. Spectral bands pointing to conformational characteristics of molecules of carotenoids but also of other compounds (phosphates, glutens and amides III) were established by the resonance Raman spectroscopy method applied to the leaf of the maize inbred line ZPLL 146. Physiological, biochemical and biophysical traits of elite maize inbred lines and their hybrids were observed in this study with a special emphasis on their efficiency of photosynthesis, productivity and suitability for broad use of nutrient values of grain and other essential biogenic matters, first of all pigments that express antioxidative properties. Relevant traits, properties and parameters of observed maize inbred lines that can be used in the process of selection are presented. These prestigious maize inbred lines were used to develop high-yielding and high-quality maize hybrids (ZP 633, ZP 735 and ZP 737) that are recognisable for their quality in human nutrition (children and the elderly), that are confirmed by medical observations related to their use in food and feed. Relevant agronomic and morphological traits of maize hybrids are presented. Moreover, results on grain structure and yields obtained in the regions of south-eastern Europe are also displayed.",
journal = "Sel'skokhozyaistvennaya Biologiya",
title = "Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value",
volume = "50",
number = "5",
pages = "600-610",
doi = "10.15389/agrobiology.2015.5.600eng",
url = "conv_1022"
}
Radenović, Č., Delić, N., Sečanski, M., Jovanović, Ž., Stanković, G.,& Popović, A.. (2015). Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value. in Sel'skokhozyaistvennaya Biologiya, 50(5), 600-610.
https://doi.org/10.15389/agrobiology.2015.5.600eng
conv_1022
Radenović Č, Delić N, Sečanski M, Jovanović Ž, Stanković G, Popović A. Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value. in Sel'skokhozyaistvennaya Biologiya. 2015;50(5):600-610.
doi:10.15389/agrobiology.2015.5.600eng
conv_1022 .
Radenović, Čedomir, Delić, Nenad, Sečanski, Mile, Jovanović, Života, Stanković, Goran, Popović, Aleksandar, "Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value" in Sel'skokhozyaistvennaya Biologiya, 50, no. 5 (2015):600-610,
https://doi.org/10.15389/agrobiology.2015.5.600eng .,
conv_1022 .

Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence

Radenović, Čedomir; Stanković, Goran; Jovanović, Života; Maksimov, Georgij; Tyutyaev, Evgenij V.; Beljanski, Miloš

(Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux, 2014)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Stanković, Goran
AU  - Jovanović, Života
AU  - Maksimov, Georgij
AU  - Tyutyaev, Evgenij V.
AU  - Beljanski, Miloš
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/542
AB  - In this paper the changes on growth, photosynthesis and water relations were tested by non-invasive fluorescence method. The applications of this method allow to determine some functional properties of prestigious maize inbred lines with erect top leaves. So the temperature dependency of delayed fluorescence intensity maximum of ZPPL 16 is observed at higher temperatures than for ZPPZ 62. This fact correlates with low values of phase transition of activation energy Ea in thylakoid membrane and accompanied by a decrease of the angle and area of the leaf, as well as with the content and the rate of water release from the seed. It seems reasonable to assume that, DF can be applied in breeding and maize hybrid seed production for the estimation of prestigious maize inbred lines and their resistance adaptability to increased and high temperatures, as well as, to drought.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence
VL  - 81
SP  - 208
EP  - 211
DO  - 10.1016/j.plaphy.2014.03.013
UR  - conv_884
ER  - 
@article{
author = "Radenović, Čedomir and Stanković, Goran and Jovanović, Života and Maksimov, Georgij and Tyutyaev, Evgenij V. and Beljanski, Miloš",
year = "2014",
abstract = "In this paper the changes on growth, photosynthesis and water relations were tested by non-invasive fluorescence method. The applications of this method allow to determine some functional properties of prestigious maize inbred lines with erect top leaves. So the temperature dependency of delayed fluorescence intensity maximum of ZPPL 16 is observed at higher temperatures than for ZPPZ 62. This fact correlates with low values of phase transition of activation energy Ea in thylakoid membrane and accompanied by a decrease of the angle and area of the leaf, as well as with the content and the rate of water release from the seed. It seems reasonable to assume that, DF can be applied in breeding and maize hybrid seed production for the estimation of prestigious maize inbred lines and their resistance adaptability to increased and high temperatures, as well as, to drought.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence",
volume = "81",
pages = "208-211",
doi = "10.1016/j.plaphy.2014.03.013",
url = "conv_884"
}
Radenović, Č., Stanković, G., Jovanović, Ž., Maksimov, G., Tyutyaev, E. V.,& Beljanski, M.. (2014). Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 81, 208-211.
https://doi.org/10.1016/j.plaphy.2014.03.013
conv_884
Radenović Č, Stanković G, Jovanović Ž, Maksimov G, Tyutyaev EV, Beljanski M. Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence. in Plant Physiology and Biochemistry. 2014;81:208-211.
doi:10.1016/j.plaphy.2014.03.013
conv_884 .
Radenović, Čedomir, Stanković, Goran, Jovanović, Života, Maksimov, Georgij, Tyutyaev, Evgenij V., Beljanski, Miloš, "Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence" in Plant Physiology and Biochemistry, 81 (2014):208-211,
https://doi.org/10.1016/j.plaphy.2014.03.013 .,
conv_884 .
4
3
4

Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize

Kostadinović, Marija; Ignjatović-Micić, Dragana; Stanković, Goran; Vančetović, Jelena; Ristić, Danijela; Mladenović-Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2014)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/526
AB  - Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize
VL  - 46
IS  - 2
SP  - 579
EP  - 590
DO  - 10.2298/GENSR1402579K
UR  - conv_888
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Vančetović, Jelena and Ristić, Danijela and Mladenović-Drinić, Snežana",
year = "2014",
abstract = "Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize",
volume = "46",
number = "2",
pages = "579-590",
doi = "10.2298/GENSR1402579K",
url = "conv_888"
}
Kostadinović, M., Ignjatović-Micić, D., Stanković, G., Vančetović, J., Ristić, D.,& Mladenović-Drinić, S.. (2014). Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika
Društvo genetičara Srbije, Beograd., 46(2), 579-590.
https://doi.org/10.2298/GENSR1402579K
conv_888
Kostadinović M, Ignjatović-Micić D, Stanković G, Vančetović J, Ristić D, Mladenović-Drinić S. Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika. 2014;46(2):579-590.
doi:10.2298/GENSR1402579K
conv_888 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Vančetović, Jelena, Ristić, Danijela, Mladenović-Drinić, Snežana, "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize" in Genetika, 46, no. 2 (2014):579-590,
https://doi.org/10.2298/GENSR1402579K .,
conv_888 .
1
1
1

Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions

Ignjatović-Micić, Dragana; Kostadinović, Marija; Stanković, Goran; Marković, Ksenija; Vančetović, Jelena; Božinović, Sofija; Anđelković, Violeta

(Maydica-Ist Sper Cerealicoltur, Bergamo, 2013)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Stanković, Goran
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Anđelković, Violeta
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/514
AB  - Quality protein maize (QPM) is high lysine (tryptophan) maize with hard endosperm and good agronomic performance. QPM was developed primarily for utilization in tropical and sub-tropical regions where maize is a staple food. Its adaptation and cultivation in temperate areas is still not fully developed, although QPM could have merits for production and consumption in developed parts of the world, especially as animal feed. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The objective of our research was initial screening of 72 hybrids (derived from crosses between QPM lines adapted to temperate environments and three commercial lines with standard kernel quality) for kernel modification, tryptophan and protein contents, quality index (QI) and grain yield. Five hybrids with high tryptophan content (0.071 to 0.081%) and yield at the level of standard hybrids (96 - 114%) were identified. Protein content ranged from 10 to 11.20%, similar to standard hybrids. QI was in the range from 0.71 to 0.74, which was better than in standard hybrids (0.57 - 0.62), but below the QPM threshold of 0.80. The percentage of good kernel modifications (type 1 and 2) was over 80% in three and over 74% in two hybrids. The results indicated the complexity of obtaining high yielding hybrids with high levels of essential amino-acids. Identified potential QPM hybrids have to be further evaluated for agronomic traits, but the results can be considered important in the context of limited information on QPM adapted to temperate environmental conditions.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions
VL  - 58
IS  - 1-4
SP  - 311
EP  - 317
UR  - conv_666
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Kostadinović, Marija and Stanković, Goran and Marković, Ksenija and Vančetović, Jelena and Božinović, Sofija and Anđelković, Violeta",
year = "2013",
abstract = "Quality protein maize (QPM) is high lysine (tryptophan) maize with hard endosperm and good agronomic performance. QPM was developed primarily for utilization in tropical and sub-tropical regions where maize is a staple food. Its adaptation and cultivation in temperate areas is still not fully developed, although QPM could have merits for production and consumption in developed parts of the world, especially as animal feed. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The objective of our research was initial screening of 72 hybrids (derived from crosses between QPM lines adapted to temperate environments and three commercial lines with standard kernel quality) for kernel modification, tryptophan and protein contents, quality index (QI) and grain yield. Five hybrids with high tryptophan content (0.071 to 0.081%) and yield at the level of standard hybrids (96 - 114%) were identified. Protein content ranged from 10 to 11.20%, similar to standard hybrids. QI was in the range from 0.71 to 0.74, which was better than in standard hybrids (0.57 - 0.62), but below the QPM threshold of 0.80. The percentage of good kernel modifications (type 1 and 2) was over 80% in three and over 74% in two hybrids. The results indicated the complexity of obtaining high yielding hybrids with high levels of essential amino-acids. Identified potential QPM hybrids have to be further evaluated for agronomic traits, but the results can be considered important in the context of limited information on QPM adapted to temperate environmental conditions.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions",
volume = "58",
number = "1-4",
pages = "311-317",
url = "conv_666"
}
Ignjatović-Micić, D., Kostadinović, M., Stanković, G., Marković, K., Vančetović, J., Božinović, S.,& Anđelković, V.. (2013). Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions. in Maydica
Maydica-Ist Sper Cerealicoltur, Bergamo., 58(1-4), 311-317.
conv_666
Ignjatović-Micić D, Kostadinović M, Stanković G, Marković K, Vančetović J, Božinović S, Anđelković V. Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions. in Maydica. 2013;58(1-4):311-317.
conv_666 .
Ignjatović-Micić, Dragana, Kostadinović, Marija, Stanković, Goran, Marković, Ksenija, Vančetović, Jelena, Božinović, Sofija, Anđelković, Violeta, "Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions" in Maydica, 58, no. 1-4 (2013):311-317,
conv_666 .
6
7

An assessment of nutritional quality of hybrid maize grain based on chemical composition

Stevanović, Milan; Mladenović-Drinić, Snežana; Dragičević, Vesna; Čamdžija, Zoran; Filipović, Milomir; Veličković, Nevena; Stanković, Goran

(Društvo genetičara Srbije, Beograd, 2012)

TY  - JOUR
AU  - Stevanović, Milan
AU  - Mladenović-Drinić, Snežana
AU  - Dragičević, Vesna
AU  - Čamdžija, Zoran
AU  - Filipović, Milomir
AU  - Veličković, Nevena
AU  - Stanković, Goran
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/439
AB  - The aim of this study was to investigate chemical composition of grain of 20 maize hybrids, from different maturity groups, to define their nutritional quality from the point of main grain constituents: starch, total proteins, soluble proteins, oil, phytate, inorganic phosphorus, and soluble phenolics, as well as mass of 1.000 grains. A set of 20 ZP hybrids, FAO 400-800, were grown in a randomized complete block design (RCBD) at Zemun Polje (Serbia), during the summer of 2009. Average content of proteins, oil and starch of analyzed maize hybrids was 10.5%, 4.7% and 80.8%, respectively. Hybrids from FAO maturity group 600 had highest grain mass (in average 419.5 g). Genetic variability in seed phytate content was observed, with values ranging from 2.64 to 3.75, averaged 3.44 g kg-1. Inorganic phosphorus (Pi) concentrations were between 0.120 µ g g-1 (ZP 805) to 0.415 µ g g-1 (ZP 588ex), averaged 0.24 µ g g-1. Content of soluble proteins varied from 54.33 to 78.74 mg g-1. The high positive correlation was observed between total proteins and oil content in hybrids, as well as between starch and phenolics content. Phytate content was positively correlated with proteins, but this correlation was not significant, while the correlation between phytate and mass of 1,000 grains was positive and significant. A negative correlation occurred between phytate and starch.
AB  - U ovom radu je analizirana hemijska kompozicija zrna 20 hibrida kukuruza različitih FAO grupa zrenja sa ciljem da se definiše njihova nutritivna vrednost na osnovu osnovnih biohemijskih komponenti zrna: skroba, proteina, ulja, fitata neorganskog fosfora, fenola i mase 1000 zrna. Eksperiment je postavljen u u blok dizajnu (RCBD) na eksperimentalnim poljima Instituta za kukuruz u Zemun Polju tokom sezone 2009. Prosečne vrednosti proteina, ulja i skroba analiziranih hibrida kukuruza iznosile su 10.5%, 4.7% and 80.8%, respektivno. Hibridi FAO grupe zrenja 600 imali su najveću masu 1000 zrna (u proseku 419.5 g). Uočena je varijabilnost u sadržaju fitina kod analiziranih hibrida i ona je iznosila od 2.64 do 3.75, sa prosečnom vrednošću od 3.44 g kg-1. Koncentracija neorganskog fosfor (Pi) se kretala od 0.120 µg g-1 (ZP 805) do 0.415 µ g g-1 (ZP 588ex), sa prosekom od 0.24 µ g g-1. Sadržaj solubilnih proteina je varirao od 54.33 do 78.74 mg g-1. Visoko pozitivna korelacija uočena je između sadržaja protein i ulja u zrnu, kao i između skroba i fenola. Fitat je bio u pozitivnoj korelaciji sa proteinima, ali ova korelacija nije bila statistički značajna, dok je korelacija između fitata i mase 1000 zrna pozitivna i signifikantna. Negativna korelacija je uočena između fitata i skroba.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - An assessment of nutritional quality of hybrid maize grain based on chemical composition
T1  - Procena nutritivnog kvaliteta hibrida kukuruza na osnovu hemijske kompozicije zrna
VL  - 44
IS  - 3
SP  - 571
EP  - 582
DO  - 10.2298/GENSR1203571S
UR  - conv_467
ER  - 
@article{
author = "Stevanović, Milan and Mladenović-Drinić, Snežana and Dragičević, Vesna and Čamdžija, Zoran and Filipović, Milomir and Veličković, Nevena and Stanković, Goran",
year = "2012",
abstract = "The aim of this study was to investigate chemical composition of grain of 20 maize hybrids, from different maturity groups, to define their nutritional quality from the point of main grain constituents: starch, total proteins, soluble proteins, oil, phytate, inorganic phosphorus, and soluble phenolics, as well as mass of 1.000 grains. A set of 20 ZP hybrids, FAO 400-800, were grown in a randomized complete block design (RCBD) at Zemun Polje (Serbia), during the summer of 2009. Average content of proteins, oil and starch of analyzed maize hybrids was 10.5%, 4.7% and 80.8%, respectively. Hybrids from FAO maturity group 600 had highest grain mass (in average 419.5 g). Genetic variability in seed phytate content was observed, with values ranging from 2.64 to 3.75, averaged 3.44 g kg-1. Inorganic phosphorus (Pi) concentrations were between 0.120 µ g g-1 (ZP 805) to 0.415 µ g g-1 (ZP 588ex), averaged 0.24 µ g g-1. Content of soluble proteins varied from 54.33 to 78.74 mg g-1. The high positive correlation was observed between total proteins and oil content in hybrids, as well as between starch and phenolics content. Phytate content was positively correlated with proteins, but this correlation was not significant, while the correlation between phytate and mass of 1,000 grains was positive and significant. A negative correlation occurred between phytate and starch., U ovom radu je analizirana hemijska kompozicija zrna 20 hibrida kukuruza različitih FAO grupa zrenja sa ciljem da se definiše njihova nutritivna vrednost na osnovu osnovnih biohemijskih komponenti zrna: skroba, proteina, ulja, fitata neorganskog fosfora, fenola i mase 1000 zrna. Eksperiment je postavljen u u blok dizajnu (RCBD) na eksperimentalnim poljima Instituta za kukuruz u Zemun Polju tokom sezone 2009. Prosečne vrednosti proteina, ulja i skroba analiziranih hibrida kukuruza iznosile su 10.5%, 4.7% and 80.8%, respektivno. Hibridi FAO grupe zrenja 600 imali su najveću masu 1000 zrna (u proseku 419.5 g). Uočena je varijabilnost u sadržaju fitina kod analiziranih hibrida i ona je iznosila od 2.64 do 3.75, sa prosečnom vrednošću od 3.44 g kg-1. Koncentracija neorganskog fosfor (Pi) se kretala od 0.120 µg g-1 (ZP 805) do 0.415 µ g g-1 (ZP 588ex), sa prosekom od 0.24 µ g g-1. Sadržaj solubilnih proteina je varirao od 54.33 do 78.74 mg g-1. Visoko pozitivna korelacija uočena je između sadržaja protein i ulja u zrnu, kao i između skroba i fenola. Fitat je bio u pozitivnoj korelaciji sa proteinima, ali ova korelacija nije bila statistički značajna, dok je korelacija između fitata i mase 1000 zrna pozitivna i signifikantna. Negativna korelacija je uočena između fitata i skroba.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "An assessment of nutritional quality of hybrid maize grain based on chemical composition, Procena nutritivnog kvaliteta hibrida kukuruza na osnovu hemijske kompozicije zrna",
volume = "44",
number = "3",
pages = "571-582",
doi = "10.2298/GENSR1203571S",
url = "conv_467"
}
Stevanović, M., Mladenović-Drinić, S., Dragičević, V., Čamdžija, Z., Filipović, M., Veličković, N.,& Stanković, G.. (2012). An assessment of nutritional quality of hybrid maize grain based on chemical composition. in Genetika
Društvo genetičara Srbije, Beograd., 44(3), 571-582.
https://doi.org/10.2298/GENSR1203571S
conv_467
Stevanović M, Mladenović-Drinić S, Dragičević V, Čamdžija Z, Filipović M, Veličković N, Stanković G. An assessment of nutritional quality of hybrid maize grain based on chemical composition. in Genetika. 2012;44(3):571-582.
doi:10.2298/GENSR1203571S
conv_467 .
Stevanović, Milan, Mladenović-Drinić, Snežana, Dragičević, Vesna, Čamdžija, Zoran, Filipović, Milomir, Veličković, Nevena, Stanković, Goran, "An assessment of nutritional quality of hybrid maize grain based on chemical composition" in Genetika, 44, no. 3 (2012):571-582,
https://doi.org/10.2298/GENSR1203571S .,
conv_467 .
1
2

Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance

Denić, Miloje; Ignjatović-Micić, Dragana; Stanković, Goran; Marković, Ksenija; Žilić, Slađana; Lazić-Jančić, Vesna; Chauque, Pedro; Fato, Pedro; Senete, Constantino; Mariote, David; Haag, Wayne

(Društvo genetičara Srbije, Beograd, 2012)

TY  - JOUR
AU  - Denić, Miloje
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Marković, Ksenija
AU  - Žilić, Slađana
AU  - Lazić-Jančić, Vesna
AU  - Chauque, Pedro
AU  - Fato, Pedro
AU  - Senete, Constantino
AU  - Mariote, David
AU  - Haag, Wayne
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/462
AB  - Due to the low biological value of proteins of common maize, it was reinitiated breeding for high protein quality maize (HQPM) using three genetic systems, namely: opaque-2 gene, endosperm modifier genes and enhancer genes, which are increasing lysine and tryptophan content in opaque-2 background In order to alleviate effect of abiotic and biotic stress factors, the genotypes with tolerance to those factors were included. Genetic resources originating from North, Central and South America, then West, Central and Southern Africa and gene bank of Maize Research Institute 'Zemun Polje' were used. Combining breeding approaches in selection of genetic resources, field plot techniques and laboratory analysis, it was created large number of early QPM varieties, inbred lines and hybrids with modified endosperm and high yield potential under poor and good growing conditions. Created lines exhibited high combining ability in conventional and non-conventional hybrids. Yield trials showed that QPM hybrids are competing with commercial hybrids of common maize.
AB  - Zbog niske biološke vrednosti proteina zrna standardnog tipa kukuruza se pristupilo stvaranju sorata i linija kukuruza visokog kvaliteta proteina (VKP) korišćenjem tri genetička sistema: opaque-2 gena, gena modifikatora endosperma i gena enhansera koji povećavaju sadržaj lizina i triptofana u opaque-2 osnovi. Da bi se istovremeno ublažilo i delovanje abiotskih i biotskih faktora stresa uključeni su i genotipovi sa tolerantnošću na ove faktore. Korišćeni su genetički resursi poreklom iz Severne, Centralne i Južne Amerike, zatim Zapadne, Centralne i Južne Afrike i resursi iz banke gena Instituta za kukuruz 'Zemun polje'. Kombinovanjem selekcionih pristupa u izboru genetičkih resursa, tehnika u poljskim i laboratorijskim uslovima stvoren je veliki broj ranih sorata, inbred linija i hibrida VKP, modifikikovanog endosperma i visokog potencijala rodnosti pod lošim i normalnim uslovima gajenja. Takođe, stvorene inbred linije su ispoljile visoku kombinacionu sposobnost u nekonvencionalnim i konvencionalnim hibridima. Poljski ogledi su pokazali da VKP hibridi konkurišu po prinosu najboljim komercijalnim hibridima standardnog tipa kukuruza.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance
T1  - Značaj genetičkih resursa iz različitih geografskih i klimatskih regiona u simultanom oplemenjivanju kukuruza na visok kvalitet proteina (VKP) i tolerantnost na stres
VL  - 44
IS  - 1
SP  - 13
EP  - 23
DO  - 10.2298/GENSR1201013D
UR  - conv_459
ER  - 
@article{
author = "Denić, Miloje and Ignjatović-Micić, Dragana and Stanković, Goran and Marković, Ksenija and Žilić, Slađana and Lazić-Jančić, Vesna and Chauque, Pedro and Fato, Pedro and Senete, Constantino and Mariote, David and Haag, Wayne",
year = "2012",
abstract = "Due to the low biological value of proteins of common maize, it was reinitiated breeding for high protein quality maize (HQPM) using three genetic systems, namely: opaque-2 gene, endosperm modifier genes and enhancer genes, which are increasing lysine and tryptophan content in opaque-2 background In order to alleviate effect of abiotic and biotic stress factors, the genotypes with tolerance to those factors were included. Genetic resources originating from North, Central and South America, then West, Central and Southern Africa and gene bank of Maize Research Institute 'Zemun Polje' were used. Combining breeding approaches in selection of genetic resources, field plot techniques and laboratory analysis, it was created large number of early QPM varieties, inbred lines and hybrids with modified endosperm and high yield potential under poor and good growing conditions. Created lines exhibited high combining ability in conventional and non-conventional hybrids. Yield trials showed that QPM hybrids are competing with commercial hybrids of common maize., Zbog niske biološke vrednosti proteina zrna standardnog tipa kukuruza se pristupilo stvaranju sorata i linija kukuruza visokog kvaliteta proteina (VKP) korišćenjem tri genetička sistema: opaque-2 gena, gena modifikatora endosperma i gena enhansera koji povećavaju sadržaj lizina i triptofana u opaque-2 osnovi. Da bi se istovremeno ublažilo i delovanje abiotskih i biotskih faktora stresa uključeni su i genotipovi sa tolerantnošću na ove faktore. Korišćeni su genetički resursi poreklom iz Severne, Centralne i Južne Amerike, zatim Zapadne, Centralne i Južne Afrike i resursi iz banke gena Instituta za kukuruz 'Zemun polje'. Kombinovanjem selekcionih pristupa u izboru genetičkih resursa, tehnika u poljskim i laboratorijskim uslovima stvoren je veliki broj ranih sorata, inbred linija i hibrida VKP, modifikikovanog endosperma i visokog potencijala rodnosti pod lošim i normalnim uslovima gajenja. Takođe, stvorene inbred linije su ispoljile visoku kombinacionu sposobnost u nekonvencionalnim i konvencionalnim hibridima. Poljski ogledi su pokazali da VKP hibridi konkurišu po prinosu najboljim komercijalnim hibridima standardnog tipa kukuruza.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance, Značaj genetičkih resursa iz različitih geografskih i klimatskih regiona u simultanom oplemenjivanju kukuruza na visok kvalitet proteina (VKP) i tolerantnost na stres",
volume = "44",
number = "1",
pages = "13-23",
doi = "10.2298/GENSR1201013D",
url = "conv_459"
}
Denić, M., Ignjatović-Micić, D., Stanković, G., Marković, K., Žilić, S., Lazić-Jančić, V., Chauque, P., Fato, P., Senete, C., Mariote, D.,& Haag, W.. (2012). Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance. in Genetika
Društvo genetičara Srbije, Beograd., 44(1), 13-23.
https://doi.org/10.2298/GENSR1201013D
conv_459
Denić M, Ignjatović-Micić D, Stanković G, Marković K, Žilić S, Lazić-Jančić V, Chauque P, Fato P, Senete C, Mariote D, Haag W. Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance. in Genetika. 2012;44(1):13-23.
doi:10.2298/GENSR1201013D
conv_459 .
Denić, Miloje, Ignjatović-Micić, Dragana, Stanković, Goran, Marković, Ksenija, Žilić, Slađana, Lazić-Jančić, Vesna, Chauque, Pedro, Fato, Pedro, Senete, Constantino, Mariote, David, Haag, Wayne, "Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance" in Genetika, 44, no. 1 (2012):13-23,
https://doi.org/10.2298/GENSR1201013D .,
conv_459 .
3
3
3

Protein quality analysis of F2 maize kernels

Kostadinović, Marija; Ignjatović-Micić, Dragana; Stanković, Goran; Mladenović-Drinić, Snežana

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2012)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Mladenović-Drinić, Snežana
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/440
AB  - Protein quality of standard maize kernels is poor, due to the low levels of two essential aminoacids - lysine and tryptophan. The opaque2 mutation which increases lysine and tryptophan content also confers an undesirable phenotype leading to low yields, soft and chalky kernels that renders seeds susceptible to storage pest and ear rots. Quality protein maize (QPM) is the maize with increased levels of these amino acids and good agronomic performances. At the Maize Research Institute, a program on creating QPM germplasm developed for conditions of temperate climatic zone is in progress. The aim of this study was to test quality traits of F2 kernels derived from the crosses between 24 QPM inbred lines with three standard inbred lines and two opaque2 lines. Most of the analyzed 108 genotypes had tryptophan content under the threshold of 0.075 set for QPM (83.5%). However, 50% had elevated tryptophan content in the range from 0.065 to 0.074. High tryptophan content was found in 16.5% of the analyzed genotypes, the highest being 0.088. Only one genotype had quality index over 0.80 (threshold for QPM). Biochemical analysis identified genotypes with good protein quality which will be further tested for agronomic performances in order to potentially be considered as QPM hybrids.
AB  - Proteini kukuruza su siromašni u sadržaju dve esencijalne aminokiseline - lizina i triptofana. Prirodna opaque2 mutacija udvostručava nivo ovih aminokiselina, ali istovremeno smanjuje prinos, čini endosperm kukuruza mekim i brašnjavim, a seme osetljivim na trulež klipa i štetočine skladištenog zrna. Kukuruz visokog kvaliteta proteina (Quality Protein Maize - QPM) se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma i istovremeno dobrim agronomskim performansama. U Institutu za kukuruz već nekoliko godina postoji program stvaranja genotipova kukuruza visokog kvaliteta proteina, adaptiranih na umereno klimatsko područje. Cilj ovog rada je bio da se ispita kvalitet proteina F2 zrna kukuruza dobijenog ukrštanjem 24 QPM inbred linija sa tri standardne inbred linije i dve opaque2 linije. Većina od 108 analiziranih genotipova (83,5%) je imala sadržaj triptofana ispod praga od 0,075, od čega je poboljšani nivo triptofana (0,065-0,074) imalo 50% genotipova. Visok sadržaj je imalo 16,5% analiziranih genotipova, od kojih je najviša vrednost bila 0,088. Samo jedan genotip je imao indeks kvaliteta proteina iznad praga od 0,80. Biohemijskom analizom utvrđeni su genotipovi poboljšanog kvaliteta čija će agronomska svojstva biti ispitana kako bi se utvrdilo da li se mogu smatrati potencijalnim QPM hibridima.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Protein quality analysis of F2 maize kernels
T1  - Biohemijska analiza F2 zrna genotipova kukuruza visokog kvaliteta proteina
VL  - 18
IS  - 1
SP  - 33
EP  - 39
UR  - conv_287
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Mladenović-Drinić, Snežana",
year = "2012",
abstract = "Protein quality of standard maize kernels is poor, due to the low levels of two essential aminoacids - lysine and tryptophan. The opaque2 mutation which increases lysine and tryptophan content also confers an undesirable phenotype leading to low yields, soft and chalky kernels that renders seeds susceptible to storage pest and ear rots. Quality protein maize (QPM) is the maize with increased levels of these amino acids and good agronomic performances. At the Maize Research Institute, a program on creating QPM germplasm developed for conditions of temperate climatic zone is in progress. The aim of this study was to test quality traits of F2 kernels derived from the crosses between 24 QPM inbred lines with three standard inbred lines and two opaque2 lines. Most of the analyzed 108 genotypes had tryptophan content under the threshold of 0.075 set for QPM (83.5%). However, 50% had elevated tryptophan content in the range from 0.065 to 0.074. High tryptophan content was found in 16.5% of the analyzed genotypes, the highest being 0.088. Only one genotype had quality index over 0.80 (threshold for QPM). Biochemical analysis identified genotypes with good protein quality which will be further tested for agronomic performances in order to potentially be considered as QPM hybrids., Proteini kukuruza su siromašni u sadržaju dve esencijalne aminokiseline - lizina i triptofana. Prirodna opaque2 mutacija udvostručava nivo ovih aminokiselina, ali istovremeno smanjuje prinos, čini endosperm kukuruza mekim i brašnjavim, a seme osetljivim na trulež klipa i štetočine skladištenog zrna. Kukuruz visokog kvaliteta proteina (Quality Protein Maize - QPM) se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma i istovremeno dobrim agronomskim performansama. U Institutu za kukuruz već nekoliko godina postoji program stvaranja genotipova kukuruza visokog kvaliteta proteina, adaptiranih na umereno klimatsko područje. Cilj ovog rada je bio da se ispita kvalitet proteina F2 zrna kukuruza dobijenog ukrštanjem 24 QPM inbred linija sa tri standardne inbred linije i dve opaque2 linije. Većina od 108 analiziranih genotipova (83,5%) je imala sadržaj triptofana ispod praga od 0,075, od čega je poboljšani nivo triptofana (0,065-0,074) imalo 50% genotipova. Visok sadržaj je imalo 16,5% analiziranih genotipova, od kojih je najviša vrednost bila 0,088. Samo jedan genotip je imao indeks kvaliteta proteina iznad praga od 0,80. Biohemijskom analizom utvrđeni su genotipovi poboljšanog kvaliteta čija će agronomska svojstva biti ispitana kako bi se utvrdilo da li se mogu smatrati potencijalnim QPM hibridima.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Protein quality analysis of F2 maize kernels, Biohemijska analiza F2 zrna genotipova kukuruza visokog kvaliteta proteina",
volume = "18",
number = "1",
pages = "33-39",
url = "conv_287"
}
Kostadinović, M., Ignjatović-Micić, D., Stanković, G.,& Mladenović-Drinić, S.. (2012). Protein quality analysis of F2 maize kernels. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 18(1), 33-39.
conv_287
Kostadinović M, Ignjatović-Micić D, Stanković G, Mladenović-Drinić S. Protein quality analysis of F2 maize kernels. in Selekcija i semenarstvo. 2012;18(1):33-39.
conv_287 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Mladenović-Drinić, Snežana, "Protein quality analysis of F2 maize kernels" in Selekcija i semenarstvo, 18, no. 1 (2012):33-39,
conv_287 .

Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties

Lević, Jelena; Petrović, Tijana; Stanković, Slavica; Stanković, Goran; Krnjaja, Vesna

(Natl Agricultural Research & Development Inst, Fundulea, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Petrović, Tijana
AU  - Stanković, Slavica
AU  - Stanković, Goran
AU  - Krnjaja, Vesna
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/412
AB  - Rot symptoms that Pyrenochaeta terrestris causes on the root of each internode of maize hybrids belonging to different maturity groups, as well as the impact of growing conditions (substrate, temperature and light) on the properties of the colony and the pycnidial formation of this fungus were studied. The large number of symptoms was obtained by various combinations of tissue colour changes (red, brown, yellow, blue and lighter or shades of these colours), changes in a particular part of the root (root top, part of the epidermis, the entire epidermis, tissue under the epidermis or the whole root) and the form of spots and streaks (shape and size). Pinkish red symptoms prevailed on younger roots, particularly from the 5th to the 7th internode. When P. terrestris was grown on potato dextrose agar (PDA) in the dark at 25 degrees C, the variability of the fungus was expressed in relation to the colour of the aerial (white, pink, grey, yellow and brown) and the substrate mycelium (purple, pink, grey, green and yellowish) and to the pycnidial formation (present or absent). The interrelationship between types of symptoms and properties of P. terrestris colonies was not determined on PDA. The fungus always produced the red purple pigment and mostly pycnidia on carnation leaf agar (CLA) at 25 degrees C and under the alternating 12 h combined light (fluorescent and near ultra violet (NUV) light)/dark conditions. These conditions are suitable for the reliable identification of the fungus.
PB  - Natl Agricultural Research & Development Inst, Fundulea
T2  - Romanian Agricultural Research
T1  - Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties
VL  - 29
SP  - 339
EP  - 347
UR  - conv_830
ER  - 
@article{
author = "Lević, Jelena and Petrović, Tijana and Stanković, Slavica and Stanković, Goran and Krnjaja, Vesna",
year = "2012",
abstract = "Rot symptoms that Pyrenochaeta terrestris causes on the root of each internode of maize hybrids belonging to different maturity groups, as well as the impact of growing conditions (substrate, temperature and light) on the properties of the colony and the pycnidial formation of this fungus were studied. The large number of symptoms was obtained by various combinations of tissue colour changes (red, brown, yellow, blue and lighter or shades of these colours), changes in a particular part of the root (root top, part of the epidermis, the entire epidermis, tissue under the epidermis or the whole root) and the form of spots and streaks (shape and size). Pinkish red symptoms prevailed on younger roots, particularly from the 5th to the 7th internode. When P. terrestris was grown on potato dextrose agar (PDA) in the dark at 25 degrees C, the variability of the fungus was expressed in relation to the colour of the aerial (white, pink, grey, yellow and brown) and the substrate mycelium (purple, pink, grey, green and yellowish) and to the pycnidial formation (present or absent). The interrelationship between types of symptoms and properties of P. terrestris colonies was not determined on PDA. The fungus always produced the red purple pigment and mostly pycnidia on carnation leaf agar (CLA) at 25 degrees C and under the alternating 12 h combined light (fluorescent and near ultra violet (NUV) light)/dark conditions. These conditions are suitable for the reliable identification of the fungus.",
publisher = "Natl Agricultural Research & Development Inst, Fundulea",
journal = "Romanian Agricultural Research",
title = "Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties",
volume = "29",
pages = "339-347",
url = "conv_830"
}
Lević, J., Petrović, T., Stanković, S., Stanković, G.,& Krnjaja, V.. (2012). Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties. in Romanian Agricultural Research
Natl Agricultural Research & Development Inst, Fundulea., 29, 339-347.
conv_830
Lević J, Petrović T, Stanković S, Stanković G, Krnjaja V. Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties. in Romanian Agricultural Research. 2012;29:339-347.
conv_830 .
Lević, Jelena, Petrović, Tijana, Stanković, Slavica, Stanković, Goran, Krnjaja, Vesna, "Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties" in Romanian Agricultural Research, 29 (2012):339-347,
conv_830 .
3
3

Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain

Stanković, Slavica; Lević, Jelena; Ivanović, Dragica; Stanković, Goran; Krnjaja, Vesna; Tančić, Sonja

(Elsevier Sci Ltd, Oxford, 2012)

TY  - JOUR
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Ivanović, Dragica
AU  - Stanković, Goran
AU  - Krnjaja, Vesna
AU  - Tančić, Sonja
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/420
AB  - The natural occurrence of fumonisin B-1 (FB1) and its co-occurrence with zearalenone (ZEA), T-2 toxin and deoxynivalenol (DON) were surveyed in 103 winter wheat samples collected after four to six-month storage in family barns from different locations in Serbia. All 103 samples were mycotoxin positive. The mean concentrations of all mycotoxins except ZEA were greater in 2005 than in 2007. FB1 was detected in 82.1% and 92.0% of all samples with ranges of 750-5400 mu g kg(-1) (mean, 2079.45 mu g kg(-1)) and 750-4900 mu g kg(-1) (mean 918.76 mu g kg(-1)) in 2005 and 2007, respectively. Moderate positive correlations were found between FB1 and DON concentrations (r = 0.56 in 2005 and r = 0.54 in 2007) and between FB1 and ZEA concentrations (r = 0.48 in 2005 and r = 0.60 in 2007), while a moderate negative correlation was detected between the production of FB1 and T-2 toxin in 2007 (r = -0.33). This is the first report of FB1 occurrence in naturally-contaminated wheat grain and its simultaneous occurrence with ZEA, DON and T-2 toxin in Serbia. Moreover, this is one of the rare reports presenting the occurrence of FB1 on wheat in the world.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain
VL  - 23
IS  - 2
SP  - 384
EP  - 388
DO  - 10.1016/j.foodcont.2011.08.003
UR  - conv_794
ER  - 
@article{
author = "Stanković, Slavica and Lević, Jelena and Ivanović, Dragica and Stanković, Goran and Krnjaja, Vesna and Tančić, Sonja",
year = "2012",
abstract = "The natural occurrence of fumonisin B-1 (FB1) and its co-occurrence with zearalenone (ZEA), T-2 toxin and deoxynivalenol (DON) were surveyed in 103 winter wheat samples collected after four to six-month storage in family barns from different locations in Serbia. All 103 samples were mycotoxin positive. The mean concentrations of all mycotoxins except ZEA were greater in 2005 than in 2007. FB1 was detected in 82.1% and 92.0% of all samples with ranges of 750-5400 mu g kg(-1) (mean, 2079.45 mu g kg(-1)) and 750-4900 mu g kg(-1) (mean 918.76 mu g kg(-1)) in 2005 and 2007, respectively. Moderate positive correlations were found between FB1 and DON concentrations (r = 0.56 in 2005 and r = 0.54 in 2007) and between FB1 and ZEA concentrations (r = 0.48 in 2005 and r = 0.60 in 2007), while a moderate negative correlation was detected between the production of FB1 and T-2 toxin in 2007 (r = -0.33). This is the first report of FB1 occurrence in naturally-contaminated wheat grain and its simultaneous occurrence with ZEA, DON and T-2 toxin in Serbia. Moreover, this is one of the rare reports presenting the occurrence of FB1 on wheat in the world.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain",
volume = "23",
number = "2",
pages = "384-388",
doi = "10.1016/j.foodcont.2011.08.003",
url = "conv_794"
}
Stanković, S., Lević, J., Ivanović, D., Stanković, G., Krnjaja, V.,& Tančić, S.. (2012). Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain. in Food Control
Elsevier Sci Ltd, Oxford., 23(2), 384-388.
https://doi.org/10.1016/j.foodcont.2011.08.003
conv_794
Stanković S, Lević J, Ivanović D, Stanković G, Krnjaja V, Tančić S. Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain. in Food Control. 2012;23(2):384-388.
doi:10.1016/j.foodcont.2011.08.003
conv_794 .
Stanković, Slavica, Lević, Jelena, Ivanović, Dragica, Stanković, Goran, Krnjaja, Vesna, Tančić, Sonja, "Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain" in Food Control, 23, no. 2 (2012):384-388,
https://doi.org/10.1016/j.foodcont.2011.08.003 .,
conv_794 .
3
46
43
45

Phenotypic and biochemical characteristics of maize genotypes during selection for high quality protein in grain

Ignjatović-Micić, Dragana; Kostadinović, Marija; Marković, Ksenija; Žilić, Slađana; Mladenović-Drinić, Snežana; Stanković, Goran

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2011)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Marković, Ksenija
AU  - Žilić, Slađana
AU  - Mladenović-Drinić, Snežana
AU  - Stanković, Goran
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/376
AB  - Maize nutritional value is very poor due to deficiency of two essential amino acids - tryptophan and lysine. It was shown than opaque2 (o2) mutations increased lysine by 69-100% and tryptophan by 66% in the endosperm. The incorporation of opaque2 into high yielding commercial cultivars failed, because of its numerous agronomic and processing problems, caused by endosperm softness. These drawbacks have been corrected in genetically improved, hard endosperm quality protein maize (QPM) - a genotype in which opaque2 has been incorporated along with associated modifiers. The breeding project at the Maize Research Institute, Zemun Polje, involves QPM x opaque2, opaque2 x QPM and standard lines x QPM crosses, with the aim to improve MRI opaque2 or convert standard lines into QPM germplasm. F5 i BC1F3 plants of these crosses were phenotyped, yield per plant was determined and endosperm modification assessment and kernel biochemical analysis (protein content, tryptophan content and quality index) were performed, with the aim to select plants for further selection process. Opaque2 x QPM progenies had the highest yield per plant - 314.3 g in BC1F3 and 230.2 g in F5. The tryptophan content and the quality index in the whole grain of QPM and opaque2 progenies were at the levels set for QPM germplasm in 72% analysed genotypes, as well as, in seven out of nine genotypes of standard lines x QPM crosses. All genotypes that had poorly modified kernels, the low tryptophan content and/or the low yield per plant will be discarded from further breeding.
AB  - Hranjiva vrednost kukuruza je dosta niska zbog nedostatka dve esencijalne aminokiseline - lizina i triptofana. Šezdesetih godina prošlog veka otkriven je prirodni opaque2 mutant kukuruza, koji u endospermu zrna sadrži 69-100% više lizina i 66% više triptofana u odnosu na standardan kukuruz. Inkorporacija opaque2 gena u visoko prinosne komercijalne hibride se pokazala neuspešnom zbog brojnih agronomskih nedostataka i problema u preradi, uzrokovanih mekim endospermom. Ovi nedostaci mogu biti prevaziđeni stvaranjem kukuruza visokog kvaliteta proteina (quality protein maize - QPM), koji pored opaque2 gena sadrži i gene modifikatore tvrdoće zrna. U Institutu za kukuruz 'Zemun Polje' kroz program stvaranja QPM germplazme formirane su F5 i BC1F3 generacije ukrštanja QPM x opaque, opaque2 x QPM i standarne linije x QPM. Utvrđene su fenotipske karakteristike, prinos po biljci, modifikacije endosperma i biohemijske karakteristike zrna (sadržaj proteina, sadržaj triptofana i indeks kvaliteta) F5 i BC1F3 biljaka, radi odabira genotipova za dalji proces selekcije. Potomstva ukrštanja opaque2 x QPM su imale najviši prinos po biljci - 314,3 g u BC1F3 i 230,2 g u F5. Sadržaj triptofana i indeks kvaliteta u celom zrnu F5 i BC1F3 generacijama ukrštanja između QPM i opaque2 linija je bio na nivou sadržaja triptofana QPM germplazme u 72% genotipova, kao i u sedam od devet ukrštanja između standardnih i QPM linija. Svi genotipovi koji su imali lošu modifikaciju zrna, nizak sadržaj triptofana i/ili nizak prinos po biljci će biti odbačeni u daljem procesu selekcije.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - Phenotypic and biochemical characteristics of maize genotypes during selection for high quality protein in grain
T1  - Fenotipske i biohemijske karakteristike genotipova kukuruza u procesu selekcije na visok kvalitet proteina u zrnu
VL  - 72
IS  - 2
SP  - 5
EP  - 13
UR  - conv_239
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Kostadinović, Marija and Marković, Ksenija and Žilić, Slađana and Mladenović-Drinić, Snežana and Stanković, Goran",
year = "2011",
abstract = "Maize nutritional value is very poor due to deficiency of two essential amino acids - tryptophan and lysine. It was shown than opaque2 (o2) mutations increased lysine by 69-100% and tryptophan by 66% in the endosperm. The incorporation of opaque2 into high yielding commercial cultivars failed, because of its numerous agronomic and processing problems, caused by endosperm softness. These drawbacks have been corrected in genetically improved, hard endosperm quality protein maize (QPM) - a genotype in which opaque2 has been incorporated along with associated modifiers. The breeding project at the Maize Research Institute, Zemun Polje, involves QPM x opaque2, opaque2 x QPM and standard lines x QPM crosses, with the aim to improve MRI opaque2 or convert standard lines into QPM germplasm. F5 i BC1F3 plants of these crosses were phenotyped, yield per plant was determined and endosperm modification assessment and kernel biochemical analysis (protein content, tryptophan content and quality index) were performed, with the aim to select plants for further selection process. Opaque2 x QPM progenies had the highest yield per plant - 314.3 g in BC1F3 and 230.2 g in F5. The tryptophan content and the quality index in the whole grain of QPM and opaque2 progenies were at the levels set for QPM germplasm in 72% analysed genotypes, as well as, in seven out of nine genotypes of standard lines x QPM crosses. All genotypes that had poorly modified kernels, the low tryptophan content and/or the low yield per plant will be discarded from further breeding., Hranjiva vrednost kukuruza je dosta niska zbog nedostatka dve esencijalne aminokiseline - lizina i triptofana. Šezdesetih godina prošlog veka otkriven je prirodni opaque2 mutant kukuruza, koji u endospermu zrna sadrži 69-100% više lizina i 66% više triptofana u odnosu na standardan kukuruz. Inkorporacija opaque2 gena u visoko prinosne komercijalne hibride se pokazala neuspešnom zbog brojnih agronomskih nedostataka i problema u preradi, uzrokovanih mekim endospermom. Ovi nedostaci mogu biti prevaziđeni stvaranjem kukuruza visokog kvaliteta proteina (quality protein maize - QPM), koji pored opaque2 gena sadrži i gene modifikatore tvrdoće zrna. U Institutu za kukuruz 'Zemun Polje' kroz program stvaranja QPM germplazme formirane su F5 i BC1F3 generacije ukrštanja QPM x opaque, opaque2 x QPM i standarne linije x QPM. Utvrđene su fenotipske karakteristike, prinos po biljci, modifikacije endosperma i biohemijske karakteristike zrna (sadržaj proteina, sadržaj triptofana i indeks kvaliteta) F5 i BC1F3 biljaka, radi odabira genotipova za dalji proces selekcije. Potomstva ukrštanja opaque2 x QPM su imale najviši prinos po biljci - 314,3 g u BC1F3 i 230,2 g u F5. Sadržaj triptofana i indeks kvaliteta u celom zrnu F5 i BC1F3 generacijama ukrštanja između QPM i opaque2 linija je bio na nivou sadržaja triptofana QPM germplazme u 72% genotipova, kao i u sedam od devet ukrštanja između standardnih i QPM linija. Svi genotipovi koji su imali lošu modifikaciju zrna, nizak sadržaj triptofana i/ili nizak prinos po biljci će biti odbačeni u daljem procesu selekcije.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "Phenotypic and biochemical characteristics of maize genotypes during selection for high quality protein in grain, Fenotipske i biohemijske karakteristike genotipova kukuruza u procesu selekcije na visok kvalitet proteina u zrnu",
volume = "72",
number = "2",
pages = "5-13",
url = "conv_239"
}
Ignjatović-Micić, D., Kostadinović, M., Marković, K., Žilić, S., Mladenović-Drinić, S.,& Stanković, G.. (2011). Phenotypic and biochemical characteristics of maize genotypes during selection for high quality protein in grain. in Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 72(2), 5-13.
conv_239
Ignjatović-Micić D, Kostadinović M, Marković K, Žilić S, Mladenović-Drinić S, Stanković G. Phenotypic and biochemical characteristics of maize genotypes during selection for high quality protein in grain. in Journal of Scientific Agricultural Research. 2011;72(2):5-13.
conv_239 .
Ignjatović-Micić, Dragana, Kostadinović, Marija, Marković, Ksenija, Žilić, Slađana, Mladenović-Drinić, Snežana, Stanković, Goran, "Phenotypic and biochemical characteristics of maize genotypes during selection for high quality protein in grain" in Journal of Scientific Agricultural Research, 72, no. 2 (2011):5-13,
conv_239 .

Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids

Lević, Jelena; Petrović, Tijana; Stanković, Slavica; Stanković, Goran; Krnjaja, Vesna

(Wiley-Blackwell, Malden, 2011)

TY  - JOUR
AU  - Lević, Jelena
AU  - Petrović, Tijana
AU  - Stanković, Slavica
AU  - Stanković, Goran
AU  - Krnjaja, Vesna
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/359
AB  - The frequency and incidence of Pyrenochaeta terrestris and symptom type on the roots of each internode of four maize hybrids of different maturity groups were studied 70 days after sowing. The fungus developed in the roots of all developed internodes (from the primary to the sixth or seventh internodes of all tested hybrids). The average frequency and incidence of P. terrestris in the roots of late and medium early maturity hybrids ranged from 29.5 to 55.2% and from 11.8 to 22.7%, respectively. The highest frequency of the fungus was at the 2nd root internode (93.3%), and its greatest incidence was detected in the mesocotyl of the medium early hybrid H-1 (56.9%). Necrosis predominated in the roots of the medium early (i.e. medium late maturity hybrids, 44.5% and 44.3%, respectively), whereas reddish pink symptoms were recorded in the roots of the late hybrids (51% and 42.5%). Because the fungus always produces a distinctive red pigment on carnation leaf agar (CLA) in a light regime, these conditions can be recommended for the reliable identification of P. terrestris, even if pycnidia are not formed. These are the first reports of the successive distribution of the fungus in each maize root internode of different hybrids, as well as the use of CLA medium in the identification of the P. terrestris.
PB  - Wiley-Blackwell, Malden
T2  - Journal of Phytopathology
T1  - Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids
VL  - 159
IS  - 6
SP  - 424
EP  - 428
DO  - 10.1111/j.1439-0434.2011.01784.x
UR  - conv_782
ER  - 
@article{
author = "Lević, Jelena and Petrović, Tijana and Stanković, Slavica and Stanković, Goran and Krnjaja, Vesna",
year = "2011",
abstract = "The frequency and incidence of Pyrenochaeta terrestris and symptom type on the roots of each internode of four maize hybrids of different maturity groups were studied 70 days after sowing. The fungus developed in the roots of all developed internodes (from the primary to the sixth or seventh internodes of all tested hybrids). The average frequency and incidence of P. terrestris in the roots of late and medium early maturity hybrids ranged from 29.5 to 55.2% and from 11.8 to 22.7%, respectively. The highest frequency of the fungus was at the 2nd root internode (93.3%), and its greatest incidence was detected in the mesocotyl of the medium early hybrid H-1 (56.9%). Necrosis predominated in the roots of the medium early (i.e. medium late maturity hybrids, 44.5% and 44.3%, respectively), whereas reddish pink symptoms were recorded in the roots of the late hybrids (51% and 42.5%). Because the fungus always produces a distinctive red pigment on carnation leaf agar (CLA) in a light regime, these conditions can be recommended for the reliable identification of P. terrestris, even if pycnidia are not formed. These are the first reports of the successive distribution of the fungus in each maize root internode of different hybrids, as well as the use of CLA medium in the identification of the P. terrestris.",
publisher = "Wiley-Blackwell, Malden",
journal = "Journal of Phytopathology",
title = "Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids",
volume = "159",
number = "6",
pages = "424-428",
doi = "10.1111/j.1439-0434.2011.01784.x",
url = "conv_782"
}
Lević, J., Petrović, T., Stanković, S., Stanković, G.,& Krnjaja, V.. (2011). Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids. in Journal of Phytopathology
Wiley-Blackwell, Malden., 159(6), 424-428.
https://doi.org/10.1111/j.1439-0434.2011.01784.x
conv_782
Lević J, Petrović T, Stanković S, Stanković G, Krnjaja V. Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids. in Journal of Phytopathology. 2011;159(6):424-428.
doi:10.1111/j.1439-0434.2011.01784.x
conv_782 .
Lević, Jelena, Petrović, Tijana, Stanković, Slavica, Stanković, Goran, Krnjaja, Vesna, "Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids" in Journal of Phytopathology, 159, no. 6 (2011):424-428,
https://doi.org/10.1111/j.1439-0434.2011.01784.x .,
conv_782 .
2
2
3

Significant genetic properties of maize inbred lines: A good basis for progress in breeding

Radenović, Čedomir; Filipović, Milomir; Stanković, Goran; Selaković, Dragojlo; Sečanski, Mile; Marković, Ksenija; Čamdžija, Zoran; Pavlov, Jovan

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2010)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Filipović, Milomir
AU  - Stanković, Goran
AU  - Selaković, Dragojlo
AU  - Sečanski, Mile
AU  - Marković, Ksenija
AU  - Čamdžija, Zoran
AU  - Pavlov, Jovan
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/325
AB  - This study conforms the hypothesis that there are elite maize inbred lines and hybrids with erect top leaves, which have a dominant property of an efficient photosynthetic and fluorescent model, that is successfully used in modern breeding programmes and the production of hybrid seed and commercial maize. This statement is supported by the displayed results on the erect position of the top leaves, the dynamics of grain dry-down during the maturation period and photosynthetic and florescence parameters: the temperature dependence of the delayed chlorophyll fluorescence intensity, the Arrhenius criterion for the determination of critical temperatures (phase transition temperatures) and the activation energies. The presented results show that properties of observed maize inbreeds and their hybrids are based on the nature of conformational and functional changes that occur in their thylakoid membranes and other chemical structures of tissues of grain and intact leaves, as well as, on positive effects in maize breeding.
AB  - U ovom radu potvrđuje se naša hipoteza da postoje elitne inbred linije kukuruza sa uspravnim položajem vršnih listova koje poseduju dominantno svojstvo efikasnog fotosintetično-fluorescentnog modela, te da se, kao takve, mogu uspešno koristiti u savremenim programima oplemenjivanja i proizvodnje hibridnog semena kukuruza. Ovoj konstataciji idu u prilog izloženi rezultati o uspravnom položaju vršnih listova prestižnih linija kukuruza, dinamici otpuštanja vode iz zrna u periodu sazrevanja i o fotosintetično-fluorescentnim pokazateljima: temperaturnoj zavisnosti intenziteta zakasnele fluorescencije hlorofila, Arenijusovom kriterijumu za određivanje kritičnih temperatura (temperature faznih prelaza) i o energiji aktivacije. Ukupno izloženi rezultati pokazuju da su svojstva prestižnih inbred linija kukuruza zasnovana na prirodi strukturnih i funkcionalnih promena, koje se odigravaju u njihovim tilakoidnim membranama i drugim hemijskim strukturama tkiva intaktnog lista i zrna, kao i na pozitivnim efektima u oplemenjivanju kukuruza.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - Significant genetic properties of maize inbred lines: A good basis for progress in breeding
T1  - Prestižna selekciona svojstva samooplodnih linija kukuruza - dobra osnova za progres u oplemenjivanju
VL  - 71
IS  - 2
SP  - 43
EP  - 61
UR  - conv_232
ER  - 
@article{
author = "Radenović, Čedomir and Filipović, Milomir and Stanković, Goran and Selaković, Dragojlo and Sečanski, Mile and Marković, Ksenija and Čamdžija, Zoran and Pavlov, Jovan",
year = "2010",
abstract = "This study conforms the hypothesis that there are elite maize inbred lines and hybrids with erect top leaves, which have a dominant property of an efficient photosynthetic and fluorescent model, that is successfully used in modern breeding programmes and the production of hybrid seed and commercial maize. This statement is supported by the displayed results on the erect position of the top leaves, the dynamics of grain dry-down during the maturation period and photosynthetic and florescence parameters: the temperature dependence of the delayed chlorophyll fluorescence intensity, the Arrhenius criterion for the determination of critical temperatures (phase transition temperatures) and the activation energies. The presented results show that properties of observed maize inbreeds and their hybrids are based on the nature of conformational and functional changes that occur in their thylakoid membranes and other chemical structures of tissues of grain and intact leaves, as well as, on positive effects in maize breeding., U ovom radu potvrđuje se naša hipoteza da postoje elitne inbred linije kukuruza sa uspravnim položajem vršnih listova koje poseduju dominantno svojstvo efikasnog fotosintetično-fluorescentnog modela, te da se, kao takve, mogu uspešno koristiti u savremenim programima oplemenjivanja i proizvodnje hibridnog semena kukuruza. Ovoj konstataciji idu u prilog izloženi rezultati o uspravnom položaju vršnih listova prestižnih linija kukuruza, dinamici otpuštanja vode iz zrna u periodu sazrevanja i o fotosintetično-fluorescentnim pokazateljima: temperaturnoj zavisnosti intenziteta zakasnele fluorescencije hlorofila, Arenijusovom kriterijumu za određivanje kritičnih temperatura (temperature faznih prelaza) i o energiji aktivacije. Ukupno izloženi rezultati pokazuju da su svojstva prestižnih inbred linija kukuruza zasnovana na prirodi strukturnih i funkcionalnih promena, koje se odigravaju u njihovim tilakoidnim membranama i drugim hemijskim strukturama tkiva intaktnog lista i zrna, kao i na pozitivnim efektima u oplemenjivanju kukuruza.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "Significant genetic properties of maize inbred lines: A good basis for progress in breeding, Prestižna selekciona svojstva samooplodnih linija kukuruza - dobra osnova za progres u oplemenjivanju",
volume = "71",
number = "2",
pages = "43-61",
url = "conv_232"
}
Radenović, Č., Filipović, M., Stanković, G., Selaković, D., Sečanski, M., Marković, K., Čamdžija, Z.,& Pavlov, J.. (2010). Significant genetic properties of maize inbred lines: A good basis for progress in breeding. in Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 71(2), 43-61.
conv_232
Radenović Č, Filipović M, Stanković G, Selaković D, Sečanski M, Marković K, Čamdžija Z, Pavlov J. Significant genetic properties of maize inbred lines: A good basis for progress in breeding. in Journal of Scientific Agricultural Research. 2010;71(2):43-61.
conv_232 .
Radenović, Čedomir, Filipović, Milomir, Stanković, Goran, Selaković, Dragojlo, Sečanski, Mile, Marković, Ksenija, Čamdžija, Zoran, Pavlov, Jovan, "Significant genetic properties of maize inbred lines: A good basis for progress in breeding" in Journal of Scientific Agricultural Research, 71, no. 2 (2010):43-61,
conv_232 .

Traits of new maize inbred lines and hybrids with efficient photosynthetic functions

Radenović, Čedomir; Filipović, Milomir; Sečanski, Mile; Stanković, Goran; Grčić, Nikola; Pavlov, Jovan; Čamdžija, Zoran

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2010)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Filipović, Milomir
AU  - Sečanski, Mile
AU  - Stanković, Goran
AU  - Grčić, Nikola
AU  - Pavlov, Jovan
AU  - Čamdžija, Zoran
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/308
AB  - This study confirms the hypothesis that there are new elite maize inbred lines and prospective hybrids, which have a dominant property of an efficient photosynthetic model that is successfully used in breeding programmes, modern technologies of the seed and commercial maize production. This statement is supported by the displayed results on the erect position of the top leaves of new maize inbred lines and photosynthetic and florescence parameters: the change of the delayed chlorophyll fluorescence intensity during its course and dynamics, the Arrhenius criterion for the determination of critical temperatures (phase transition temperatures) and the activation energies, as a measure of conformational changes in chloroplasts the thylakoid membrane. Furthermore, a grain structure, including its physical and chemical parameters, of new maize inbred lines was analysed in the present study. Also, relevant breeding, seed production and technological traits, properties and parameters of new inbred lines and prospective maize hybrids were observed in the present study. The presented results show that properties of new inbred lines and prospective maize hybrids are based on the nature of conformational and functional changes that occur in their chloroplasts and thylakoid membranes, as well as, on progressive effects in maize breeding, modern hybrid seed production and the commercial maize production.
AB  - U ovom radu vršena su proučavanja novih elitnih inbred linija kukuruza: ZPPL 218 i ZPPL 318 i perspektivnih hibrida kukuruza ZP 600, ZP 606 i ZP 666 stvorenih iz njih. Pokazano je da se potvrđuje naša hipoteza da nove elitne inbred linije i perspektivni hibridi kukuruza poseduju dominantno svojstvo efikasnog fotosintetičnog modela što se uspešno koristi u oplemenjivanju, savremenim tehnologijama proizvodnje hibridnog semena i merkantilnog kukuruza. Ovoj konstataciji idu u prilog izloženi rezultati o uspravnom položaju vršnih listova novih inbred linija kukuruza i o fotosintetički-fluorescentnim pokazateljima: promeni intenziteta zakasnele fluorescencije hlorofila u njenom toku i dinamici, Arenijusovom kriterijumu za određivanje kritičnih temperature (temperature faznih prelaza) i o energiji aktivacije kao meri strukturnih promena u hloroplastima i tilakoidnoj membrani. U radu se analiziraju struktura zrna uključujući i njegove fizičke i hemijske pokazatelje novih inbred linija kukuruza. Isto tako, u radu se razmatraju relevantna selekcionarska, semenarska i tehnološka svojstva, karakteristike i parametri novih inbred linija i perspektivnih hibrida kukuruza. Ukupno izloženi rezultati pokazuju da su svojstva novih inbred linija i perspektivnih hibrida kukuruza, zasnovana na prirodi strukturnih i funkcionalnih promena koje se odigravaju u hloroplastima i tilakoidnim membranama, kao i na progresivnim efektima u oplemenjivanju, savremenoj proizvodnji hibridnog semena i merkatilnog kukuruza.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - Traits of new maize inbred lines and hybrids with efficient photosynthetic functions
T1  - Svojstva novih inbred linija i hibrida kukuruza sa efikasnim fotosintetičkim funkcijama
VL  - 71
IS  - 4
SP  - 65
EP  - 88
UR  - conv_238
ER  - 
@article{
author = "Radenović, Čedomir and Filipović, Milomir and Sečanski, Mile and Stanković, Goran and Grčić, Nikola and Pavlov, Jovan and Čamdžija, Zoran",
year = "2010",
abstract = "This study confirms the hypothesis that there are new elite maize inbred lines and prospective hybrids, which have a dominant property of an efficient photosynthetic model that is successfully used in breeding programmes, modern technologies of the seed and commercial maize production. This statement is supported by the displayed results on the erect position of the top leaves of new maize inbred lines and photosynthetic and florescence parameters: the change of the delayed chlorophyll fluorescence intensity during its course and dynamics, the Arrhenius criterion for the determination of critical temperatures (phase transition temperatures) and the activation energies, as a measure of conformational changes in chloroplasts the thylakoid membrane. Furthermore, a grain structure, including its physical and chemical parameters, of new maize inbred lines was analysed in the present study. Also, relevant breeding, seed production and technological traits, properties and parameters of new inbred lines and prospective maize hybrids were observed in the present study. The presented results show that properties of new inbred lines and prospective maize hybrids are based on the nature of conformational and functional changes that occur in their chloroplasts and thylakoid membranes, as well as, on progressive effects in maize breeding, modern hybrid seed production and the commercial maize production., U ovom radu vršena su proučavanja novih elitnih inbred linija kukuruza: ZPPL 218 i ZPPL 318 i perspektivnih hibrida kukuruza ZP 600, ZP 606 i ZP 666 stvorenih iz njih. Pokazano je da se potvrđuje naša hipoteza da nove elitne inbred linije i perspektivni hibridi kukuruza poseduju dominantno svojstvo efikasnog fotosintetičnog modela što se uspešno koristi u oplemenjivanju, savremenim tehnologijama proizvodnje hibridnog semena i merkantilnog kukuruza. Ovoj konstataciji idu u prilog izloženi rezultati o uspravnom položaju vršnih listova novih inbred linija kukuruza i o fotosintetički-fluorescentnim pokazateljima: promeni intenziteta zakasnele fluorescencije hlorofila u njenom toku i dinamici, Arenijusovom kriterijumu za određivanje kritičnih temperature (temperature faznih prelaza) i o energiji aktivacije kao meri strukturnih promena u hloroplastima i tilakoidnoj membrani. U radu se analiziraju struktura zrna uključujući i njegove fizičke i hemijske pokazatelje novih inbred linija kukuruza. Isto tako, u radu se razmatraju relevantna selekcionarska, semenarska i tehnološka svojstva, karakteristike i parametri novih inbred linija i perspektivnih hibrida kukuruza. Ukupno izloženi rezultati pokazuju da su svojstva novih inbred linija i perspektivnih hibrida kukuruza, zasnovana na prirodi strukturnih i funkcionalnih promena koje se odigravaju u hloroplastima i tilakoidnim membranama, kao i na progresivnim efektima u oplemenjivanju, savremenoj proizvodnji hibridnog semena i merkatilnog kukuruza.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "Traits of new maize inbred lines and hybrids with efficient photosynthetic functions, Svojstva novih inbred linija i hibrida kukuruza sa efikasnim fotosintetičkim funkcijama",
volume = "71",
number = "4",
pages = "65-88",
url = "conv_238"
}
Radenović, Č., Filipović, M., Sečanski, M., Stanković, G., Grčić, N., Pavlov, J.,& Čamdžija, Z.. (2010). Traits of new maize inbred lines and hybrids with efficient photosynthetic functions. in Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 71(4), 65-88.
conv_238
Radenović Č, Filipović M, Sečanski M, Stanković G, Grčić N, Pavlov J, Čamdžija Z. Traits of new maize inbred lines and hybrids with efficient photosynthetic functions. in Journal of Scientific Agricultural Research. 2010;71(4):65-88.
conv_238 .
Radenović, Čedomir, Filipović, Milomir, Sečanski, Mile, Stanković, Goran, Grčić, Nikola, Pavlov, Jovan, Čamdžija, Zoran, "Traits of new maize inbred lines and hybrids with efficient photosynthetic functions" in Journal of Scientific Agricultural Research, 71, no. 4 (2010):65-88,
conv_238 .

Protein and tryptophan content in kernels of maize hybrids

Žilić, Slađana; Ignjatović-Micić, Dragana; Saratlić, Goran; Stanković, Goran; Delić, Nenad; Filipović, Milomir; Kresović, Branka

(Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad, 2010)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Ignjatović-Micić, Dragana
AU  - Saratlić, Goran
AU  - Stanković, Goran
AU  - Delić, Nenad
AU  - Filipović, Milomir
AU  - Kresović, Branka
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/320
AB  - In this work, proteins and tryptophan content were analyzed in kernels of semi-flint, semi-dent, dent and specialty ZP maize hybrids. Protein content, as well as protein fraction, differed significantly among the analyzed maize genotypes. Globulin was the lowest fraction in kernels of all maize hybrids (6.69-11.03% of total protein). The content of albumin was the highest in sweet hybrids ZP 441su and ZP 461su i.e. 20.27% and 19.76% of total protein, respectively. α-Zein and G-3 glutelin were dominant protein fractions in all maize genotypes. The highest content of α-zein and G-3 glutelin was in the kernels of popping maize hybrid ZP 611k, i.e. 29.25 and 25.71% of total protein, respectively. All hybrids could be classified according to the tryptophan content in three groups - over 0.08% (sweet and waxy hybrids), from 0.06 to 0.07% (three standard dent hybrids, one semi-flint and two semident hybrid) and from 0.05 to 0.06% (two semi-flint, one semi-dent hybrids and popping).
AB  - U ovom radu ispitivan je sadržaj rastvorljivih proteina i triptofana u zrnu polutvrdunaca, poluzubana, zubana i specifičnih ZP hibrida kukuruza. Sadržaj ukupnih proteina, kao i sadržaj rastvorljivih proteinskih frakcija se statistički razlikovao između analiziranih genotipova kukuruza. Globulin je bio najmanjie zastupljena frakcija u kukuruznom zrnu (6.69-11.03% od ukupnih proteina). Sadržaj albuminske frakcije u zrnu hibrida šećeraca ZP 441su i ZP 461su iznosio je 20.27% i 19.76% od ukupnih proteina, dok je u zrnu ostalih hibrida bio značajno niži. α-zein i G3-glutelin bile su dominantne frakcije u zrnu svih ispitivanih genotipova kukuruza. Najviši sadržaj α-zeina i G3-glutelina bio je u zrnu kukuruza kokičara ZP 611k i iznosio je 29.25% i 25.71% od ukupnih proteina. Prema sadržaju triptofana svi analizirani hibridi mogu se podeliti u tri grupe: preko 0.08% (šećerci i voksi hibridi - ZP 441su, ZP 461su i ZP 704wx), od 0.06 do 0.07% (tri standardna zubana - ZP 434, ZP 544, ZP 688, poluzubani - ZP 643, ZP 717 i polutvrdunac ZP Prado), od 0.05 do 0.06% (dva polutvrdunca - ZP 209, ZP 745, jedan poluzuban i kokičar - ZP 505, ZP 611k). PR Projekat Ministarstva nauke Republike Srbije, br. TR-20039.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Protein and tryptophan content in kernels of maize hybrids
T1  - Sadržaj proteina i triptofana u zrnu hibrida kukuruza
VL  - 59
IS  - 1-2
SP  - 56
EP  - 64
UR  - conv_10
ER  - 
@article{
author = "Žilić, Slađana and Ignjatović-Micić, Dragana and Saratlić, Goran and Stanković, Goran and Delić, Nenad and Filipović, Milomir and Kresović, Branka",
year = "2010",
abstract = "In this work, proteins and tryptophan content were analyzed in kernels of semi-flint, semi-dent, dent and specialty ZP maize hybrids. Protein content, as well as protein fraction, differed significantly among the analyzed maize genotypes. Globulin was the lowest fraction in kernels of all maize hybrids (6.69-11.03% of total protein). The content of albumin was the highest in sweet hybrids ZP 441su and ZP 461su i.e. 20.27% and 19.76% of total protein, respectively. α-Zein and G-3 glutelin were dominant protein fractions in all maize genotypes. The highest content of α-zein and G-3 glutelin was in the kernels of popping maize hybrid ZP 611k, i.e. 29.25 and 25.71% of total protein, respectively. All hybrids could be classified according to the tryptophan content in three groups - over 0.08% (sweet and waxy hybrids), from 0.06 to 0.07% (three standard dent hybrids, one semi-flint and two semident hybrid) and from 0.05 to 0.06% (two semi-flint, one semi-dent hybrids and popping)., U ovom radu ispitivan je sadržaj rastvorljivih proteina i triptofana u zrnu polutvrdunaca, poluzubana, zubana i specifičnih ZP hibrida kukuruza. Sadržaj ukupnih proteina, kao i sadržaj rastvorljivih proteinskih frakcija se statistički razlikovao između analiziranih genotipova kukuruza. Globulin je bio najmanjie zastupljena frakcija u kukuruznom zrnu (6.69-11.03% od ukupnih proteina). Sadržaj albuminske frakcije u zrnu hibrida šećeraca ZP 441su i ZP 461su iznosio je 20.27% i 19.76% od ukupnih proteina, dok je u zrnu ostalih hibrida bio značajno niži. α-zein i G3-glutelin bile su dominantne frakcije u zrnu svih ispitivanih genotipova kukuruza. Najviši sadržaj α-zeina i G3-glutelina bio je u zrnu kukuruza kokičara ZP 611k i iznosio je 29.25% i 25.71% od ukupnih proteina. Prema sadržaju triptofana svi analizirani hibridi mogu se podeliti u tri grupe: preko 0.08% (šećerci i voksi hibridi - ZP 441su, ZP 461su i ZP 704wx), od 0.06 do 0.07% (tri standardna zubana - ZP 434, ZP 544, ZP 688, poluzubani - ZP 643, ZP 717 i polutvrdunac ZP Prado), od 0.05 do 0.06% (dva polutvrdunca - ZP 209, ZP 745, jedan poluzuban i kokičar - ZP 505, ZP 611k). PR Projekat Ministarstva nauke Republike Srbije, br. TR-20039.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Protein and tryptophan content in kernels of maize hybrids, Sadržaj proteina i triptofana u zrnu hibrida kukuruza",
volume = "59",
number = "1-2",
pages = "56-64",
url = "conv_10"
}
Žilić, S., Ignjatović-Micić, D., Saratlić, G., Stanković, G., Delić, N., Filipović, M.,& Kresović, B.. (2010). Protein and tryptophan content in kernels of maize hybrids. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 59(1-2), 56-64.
conv_10
Žilić S, Ignjatović-Micić D, Saratlić G, Stanković G, Delić N, Filipović M, Kresović B. Protein and tryptophan content in kernels of maize hybrids. in Savremena poljoprivreda. 2010;59(1-2):56-64.
conv_10 .
Žilić, Slađana, Ignjatović-Micić, Dragana, Saratlić, Goran, Stanković, Goran, Delić, Nenad, Filipović, Milomir, Kresović, Branka, "Protein and tryptophan content in kernels of maize hybrids" in Savremena poljoprivreda, 59, no. 1-2 (2010):56-64,
conv_10 .

The content of antioxidants in sunflower seed and kernel

Žilić, Slađana; Maksimović-Dragišić, J.; Maksimović, V.; Maksimović, M.; Basić, Zorica; Crevar, Miloš; Stanković, Goran

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2010)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Maksimović-Dragišić, J.
AU  - Maksimović, V.
AU  - Maksimović, M.
AU  - Basić, Zorica
AU  - Crevar, Miloš
AU  - Stanković, Goran
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/328
AB  - The primary objective of this research was to determine differences among investigated sunflower genotypes and whether the analyzed hybrids could be sources of phenols and tocopherols important for storage stability of sunflower seeds and their derived products. DPPH• radical scavenging activity, the content of phenolic components and tocopherols (α-, β-, γ-, and δ-) in seeds and kernels of three sunflower hybrids were analyzed. In the present study, six different phenolic compounds were separated by the HPLC analysis. Chlorogenic acid was the most abundant phenol. The chlorogenic acid content strongly correlated with total phenols (r=0.93). Other marked phenolics were caffeic acid, ferulic acid, rosmarinic acid, myricetin and rutin. The total tocopherols were significantly higher (P lt 0.05) in kernels than in seeds of all sunflower hybrids. Concentrations in sunflower seeds ranged from 200.67 to 220.05 μg/g and from 256.62 to 267.49 μg/g in sunflower kernels where α-tocopherol was the dominant isomer in all samples. The α-tocopherol content was 98% of averaged of the total tocopherols in all analysed samples. All these nutrients with antioxidant properties influenced the capacity of DPPH• scavenging. Accordingly, sunflower kernels had a higher DPPH• scavenging activity, and a higher nutritive value than sunflower seeds.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - The content of antioxidants in sunflower seed and kernel
VL  - 33
IS  - 52
SP  - 75
EP  - 84
DO  - 10.2298/HEL1052075Z
UR  - conv_510
ER  - 
@article{
author = "Žilić, Slađana and Maksimović-Dragišić, J. and Maksimović, V. and Maksimović, M. and Basić, Zorica and Crevar, Miloš and Stanković, Goran",
year = "2010",
abstract = "The primary objective of this research was to determine differences among investigated sunflower genotypes and whether the analyzed hybrids could be sources of phenols and tocopherols important for storage stability of sunflower seeds and their derived products. DPPH• radical scavenging activity, the content of phenolic components and tocopherols (α-, β-, γ-, and δ-) in seeds and kernels of three sunflower hybrids were analyzed. In the present study, six different phenolic compounds were separated by the HPLC analysis. Chlorogenic acid was the most abundant phenol. The chlorogenic acid content strongly correlated with total phenols (r=0.93). Other marked phenolics were caffeic acid, ferulic acid, rosmarinic acid, myricetin and rutin. The total tocopherols were significantly higher (P lt 0.05) in kernels than in seeds of all sunflower hybrids. Concentrations in sunflower seeds ranged from 200.67 to 220.05 μg/g and from 256.62 to 267.49 μg/g in sunflower kernels where α-tocopherol was the dominant isomer in all samples. The α-tocopherol content was 98% of averaged of the total tocopherols in all analysed samples. All these nutrients with antioxidant properties influenced the capacity of DPPH• scavenging. Accordingly, sunflower kernels had a higher DPPH• scavenging activity, and a higher nutritive value than sunflower seeds.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "The content of antioxidants in sunflower seed and kernel",
volume = "33",
number = "52",
pages = "75-84",
doi = "10.2298/HEL1052075Z",
url = "conv_510"
}
Žilić, S., Maksimović-Dragišić, J., Maksimović, V., Maksimović, M., Basić, Z., Crevar, M.,& Stanković, G.. (2010). The content of antioxidants in sunflower seed and kernel. in Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 33(52), 75-84.
https://doi.org/10.2298/HEL1052075Z
conv_510
Žilić S, Maksimović-Dragišić J, Maksimović V, Maksimović M, Basić Z, Crevar M, Stanković G. The content of antioxidants in sunflower seed and kernel. in Helia. 2010;33(52):75-84.
doi:10.2298/HEL1052075Z
conv_510 .
Žilić, Slađana, Maksimović-Dragišić, J., Maksimović, V., Maksimović, M., Basić, Zorica, Crevar, Miloš, Stanković, Goran, "The content of antioxidants in sunflower seed and kernel" in Helia, 33, no. 52 (2010):75-84,
https://doi.org/10.2298/HEL1052075Z .,
conv_510 .
13
19

Kernel modifications and tryptophan content in QPM segregating generations

Ignjatović-Micić, Dragana; Stanković, Goran; Marković, Ksenija; Mladenović-Drinić, Snežana; Lazić-Jančić, Vesna; Denić, Miloje

(Društvo genetičara Srbije, Beograd, 2010)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Marković, Ksenija
AU  - Mladenović-Drinić, Snežana
AU  - Lazić-Jančić, Vesna
AU  - Denić, Miloje
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/303
AB  - Maize has poor nutritional value due to deficiency of two essential amino acids - tryptophan and lysine. Although recessive opaque2 (o2) mutation significantly increases their content in the endosperm, incorporation of opaque2 into high yielding cultivars was not commercially successful, because of its numerous agronomic and processing problems due to soft endosperm. Quality protein maize - QPM has lately been introduced as opaque2 maize with improved endosperm hardness and improved agronomic traits, but mostly within tropical and subtropical germplasm. The ongoing breeding project at MRI includes improvement of MRI opaque2 lines and conversion of standard lines to QPM germplasm. The main selection steps in QPM breeding involve assessing kernel modifications and tryptophan level in each generation. Herein, we present the results of the analysis for these traits on F3 and BC1F1 generations of QPM x opaque2, opaque2 x QPM and standard lines x QPM crosses. The results showed that the majority the genotypes had kernel types 2 and 3 (good modifications). The whole grain tryptophan content in F3 and BC1F1 genotypes of crosses between QPM and opaque2 germplasm was at the quality protein level, with a few exceptions. All BC1F1 genotypes of standard lines x QPM had tryptophan content in the range of normal maize, while majority of F3 genotypes had tryptophan content at level of QPM. The progeny (with increased tryptophan levels) of QPM and opaque2 crosses had significantly higher tryptophan content compared to the progeny of crosses between standard and QPM lines - 0.098 to 0.114 and 0.080, respectively. All genotypes that had poorly modified kernels and/or low tryptophan content will be discarded from further breeding.
AB  - Iako recesivna opaque2 (o2) mutacija značajno povećava sadržaj lizina i triptofana u zrnu kukuruza, inkorporacija opaque2 u visoko prinosne genotipove nije uspela, zbog brojnih problema koji su se javili kao posledica mekog endosperma. Kukuruz visokog kvaliteta proteina (QPM) je opaque2 kukuruz sa poboljšanom tvrdoćom zrna i dobrim agronomskim performansama, stvoren prvenstveno od tropske i subtropske germplazme. U Institutu za kukuruz je u toku projekat koji za cilj ima poboljšanje sopstvenih opaque2 linija i prevođenje standardnih linija u QPM kukuruz. U ovom radu predstavljamo rezultate analiza modifikacije zrna i sadržaja triptofana u F3 i BC1F1 generacijama ukrštanja QPM x opaque2, opaque2 x QPM i standardne linije x QPM. Većina genotipova je imala zrno tipa 2 i tipa3 (dobre modifikacije). Sadržaj triptofana u celom zrnu genotipova ukrštanja QPM i opaque2 linija je bio na nivou karakterističnom za QPM, sa nekoliko izuzetaka. Sadržaj triptofana u potomstvu ukrštanja standardne linije x QPM je bio povećan kod većine F3 genotipova. Potomstvo (sa povećanim sadržajem triptofana) ukrštanja QPM i opaque2 linija je imalo znatno veći sadržaj ove aminokiseline u odnosu na potostvo ukrštanja standardne linije x QPM - - od 0.098 do 0.114 prema 0.080. Genotipovi sa lošim modifikacija zrna i/ili niskim sadržajem triptofana ce biti eleminisani iz daljeg procesa selekcije.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Kernel modifications and tryptophan content in QPM segregating generations
T1  - Modifikacija zrna i sadržaj triptofana u segregirajućim generacijama kukuruza visokog kvaliteta proteina
VL  - 42
IS  - 2
SP  - 267
EP  - 277
DO  - 10.2298/GENSR1002267M
UR  - conv_444
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Stanković, Goran and Marković, Ksenija and Mladenović-Drinić, Snežana and Lazić-Jančić, Vesna and Denić, Miloje",
year = "2010",
abstract = "Maize has poor nutritional value due to deficiency of two essential amino acids - tryptophan and lysine. Although recessive opaque2 (o2) mutation significantly increases their content in the endosperm, incorporation of opaque2 into high yielding cultivars was not commercially successful, because of its numerous agronomic and processing problems due to soft endosperm. Quality protein maize - QPM has lately been introduced as opaque2 maize with improved endosperm hardness and improved agronomic traits, but mostly within tropical and subtropical germplasm. The ongoing breeding project at MRI includes improvement of MRI opaque2 lines and conversion of standard lines to QPM germplasm. The main selection steps in QPM breeding involve assessing kernel modifications and tryptophan level in each generation. Herein, we present the results of the analysis for these traits on F3 and BC1F1 generations of QPM x opaque2, opaque2 x QPM and standard lines x QPM crosses. The results showed that the majority the genotypes had kernel types 2 and 3 (good modifications). The whole grain tryptophan content in F3 and BC1F1 genotypes of crosses between QPM and opaque2 germplasm was at the quality protein level, with a few exceptions. All BC1F1 genotypes of standard lines x QPM had tryptophan content in the range of normal maize, while majority of F3 genotypes had tryptophan content at level of QPM. The progeny (with increased tryptophan levels) of QPM and opaque2 crosses had significantly higher tryptophan content compared to the progeny of crosses between standard and QPM lines - 0.098 to 0.114 and 0.080, respectively. All genotypes that had poorly modified kernels and/or low tryptophan content will be discarded from further breeding., Iako recesivna opaque2 (o2) mutacija značajno povećava sadržaj lizina i triptofana u zrnu kukuruza, inkorporacija opaque2 u visoko prinosne genotipove nije uspela, zbog brojnih problema koji su se javili kao posledica mekog endosperma. Kukuruz visokog kvaliteta proteina (QPM) je opaque2 kukuruz sa poboljšanom tvrdoćom zrna i dobrim agronomskim performansama, stvoren prvenstveno od tropske i subtropske germplazme. U Institutu za kukuruz je u toku projekat koji za cilj ima poboljšanje sopstvenih opaque2 linija i prevođenje standardnih linija u QPM kukuruz. U ovom radu predstavljamo rezultate analiza modifikacije zrna i sadržaja triptofana u F3 i BC1F1 generacijama ukrštanja QPM x opaque2, opaque2 x QPM i standardne linije x QPM. Većina genotipova je imala zrno tipa 2 i tipa3 (dobre modifikacije). Sadržaj triptofana u celom zrnu genotipova ukrštanja QPM i opaque2 linija je bio na nivou karakterističnom za QPM, sa nekoliko izuzetaka. Sadržaj triptofana u potomstvu ukrštanja standardne linije x QPM je bio povećan kod većine F3 genotipova. Potomstvo (sa povećanim sadržajem triptofana) ukrštanja QPM i opaque2 linija je imalo znatno veći sadržaj ove aminokiseline u odnosu na potostvo ukrštanja standardne linije x QPM - - od 0.098 do 0.114 prema 0.080. Genotipovi sa lošim modifikacija zrna i/ili niskim sadržajem triptofana ce biti eleminisani iz daljeg procesa selekcije.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Kernel modifications and tryptophan content in QPM segregating generations, Modifikacija zrna i sadržaj triptofana u segregirajućim generacijama kukuruza visokog kvaliteta proteina",
volume = "42",
number = "2",
pages = "267-277",
doi = "10.2298/GENSR1002267M",
url = "conv_444"
}
Ignjatović-Micić, D., Stanković, G., Marković, K., Mladenović-Drinić, S., Lazić-Jančić, V.,& Denić, M.. (2010). Kernel modifications and tryptophan content in QPM segregating generations. in Genetika
Društvo genetičara Srbije, Beograd., 42(2), 267-277.
https://doi.org/10.2298/GENSR1002267M
conv_444
Ignjatović-Micić D, Stanković G, Marković K, Mladenović-Drinić S, Lazić-Jančić V, Denić M. Kernel modifications and tryptophan content in QPM segregating generations. in Genetika. 2010;42(2):267-277.
doi:10.2298/GENSR1002267M
conv_444 .
Ignjatović-Micić, Dragana, Stanković, Goran, Marković, Ksenija, Mladenović-Drinić, Snežana, Lazić-Jančić, Vesna, Denić, Miloje, "Kernel modifications and tryptophan content in QPM segregating generations" in Genetika, 42, no. 2 (2010):267-277,
https://doi.org/10.2298/GENSR1002267M .,
conv_444 .
2
3
5

Evaluation of Serbian commercial maize hybrid tolerance to feeding by larval western corn rootworm (diabrotica virgifera virgifera leconte) using me novel 'difference approach'

Kadlicko, S. R.; Tollefson, J. J.; Prasifka, J. R.; Bača, Franja; Stanković, Goran; Delić, Nenad

(Maydica-Ist Sper Cerealicoltur, Bergamo, 2010)

TY  - JOUR
AU  - Kadlicko, S. R.
AU  - Tollefson, J. J.
AU  - Prasifka, J. R.
AU  - Bača, Franja
AU  - Stanković, Goran
AU  - Delić, Nenad
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/349
AB  - Since the discovery of the pest in 1992, western corn rootworm (Diabrotica virgifera virgifera LeConte (WCR)) populations in Serbia have successfully been kept low with crop rotation. This has reduced the efficiency of screening maize for WCR resistance. A cooperative project between Iowa State University and the Maize Research Institute, Zemun Polje evaluated 13 Serbian commercial maize varieties in Ames, Iowa over a two-year period using the difference approach. Maize hybrids were planted on trap crops where high WCR populations were assumed. Hybrids were evaluated for WCR resistance using a randomized complete block design with four replications. Treatments were paired-rows arranged in split plots with one row in each pair treated with insecticide and the other row left untreated. WCR injury was evaluated using a rating of root size and root re-growth (1-6 scale), root injury (0-3 Node-Injury Scale), root mass, lodging, and yield. The results indicated significant differences among the Serbian hybrids in the presence of moderate-to-high levels of western corn rootworms. The relative benefit of insecticide treatments for maize hybrids was a useful tool in evaluating resistant germplasm. However, conducting analyses on relative differences between insecticide treated and untreated plots was not as effective at detecting differences as comparing the plots independently.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - Evaluation of Serbian commercial maize hybrid tolerance to feeding by larval western corn rootworm (diabrotica virgifera virgifera leconte) using me novel 'difference approach'
VL  - 55
IS  - 3-4
SP  - 179
EP  - 185
UR  - conv_780
ER  - 
@article{
author = "Kadlicko, S. R. and Tollefson, J. J. and Prasifka, J. R. and Bača, Franja and Stanković, Goran and Delić, Nenad",
year = "2010",
abstract = "Since the discovery of the pest in 1992, western corn rootworm (Diabrotica virgifera virgifera LeConte (WCR)) populations in Serbia have successfully been kept low with crop rotation. This has reduced the efficiency of screening maize for WCR resistance. A cooperative project between Iowa State University and the Maize Research Institute, Zemun Polje evaluated 13 Serbian commercial maize varieties in Ames, Iowa over a two-year period using the difference approach. Maize hybrids were planted on trap crops where high WCR populations were assumed. Hybrids were evaluated for WCR resistance using a randomized complete block design with four replications. Treatments were paired-rows arranged in split plots with one row in each pair treated with insecticide and the other row left untreated. WCR injury was evaluated using a rating of root size and root re-growth (1-6 scale), root injury (0-3 Node-Injury Scale), root mass, lodging, and yield. The results indicated significant differences among the Serbian hybrids in the presence of moderate-to-high levels of western corn rootworms. The relative benefit of insecticide treatments for maize hybrids was a useful tool in evaluating resistant germplasm. However, conducting analyses on relative differences between insecticide treated and untreated plots was not as effective at detecting differences as comparing the plots independently.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "Evaluation of Serbian commercial maize hybrid tolerance to feeding by larval western corn rootworm (diabrotica virgifera virgifera leconte) using me novel 'difference approach'",
volume = "55",
number = "3-4",
pages = "179-185",
url = "conv_780"
}
Kadlicko, S. R., Tollefson, J. J., Prasifka, J. R., Bača, F., Stanković, G.,& Delić, N.. (2010). Evaluation of Serbian commercial maize hybrid tolerance to feeding by larval western corn rootworm (diabrotica virgifera virgifera leconte) using me novel 'difference approach'. in Maydica
Maydica-Ist Sper Cerealicoltur, Bergamo., 55(3-4), 179-185.
conv_780
Kadlicko SR, Tollefson JJ, Prasifka JR, Bača F, Stanković G, Delić N. Evaluation of Serbian commercial maize hybrid tolerance to feeding by larval western corn rootworm (diabrotica virgifera virgifera leconte) using me novel 'difference approach'. in Maydica. 2010;55(3-4):179-185.
conv_780 .
Kadlicko, S. R., Tollefson, J. J., Prasifka, J. R., Bača, Franja, Stanković, Goran, Delić, Nenad, "Evaluation of Serbian commercial maize hybrid tolerance to feeding by larval western corn rootworm (diabrotica virgifera virgifera leconte) using me novel 'difference approach'" in Maydica, 55, no. 3-4 (2010):179-185,
conv_780 .
3
3

Use of non parametric statistics in estimation of genotypes stability

Delić, Nenad; Stanković, Goran; Konstantinov, Kosana

(Maydica-Ist Sper Cerealicoltur, Bergamo, 2009)

TY  - JOUR
AU  - Delić, Nenad
AU  - Stanković, Goran
AU  - Konstantinov, Kosana
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/277
AB  - The Mostly used, classical parametric approaches for an analysis of genotype x environment interaction are based on several assumptions: normality of: the distribution, homogeneity of variances, additivity. If some of mentioned assumptions are not fulfilled, the validity of these methods may be questionable. By use of nonparametric methods, which are simple and easy for analysis, all of the mentioned assumptions are avoided. in this paper we used several of non parametric techniques for analysis of genotype x environment interaction for grain yield of 24 maize (Zea mays Q hybrids through two locations in two years. Values of the stability measures shown that hybrids with the highest grain yield in the majority of cases were not the most stable. So as the value of the relative deviation in relation to the average rank classified the lowest yielding (4.9 t ha(-1)) genotype PR36B08 as the most stable genotype (S-i((3)) = 0.12). The interrelations of numerical values among methods, indicating the genotype x environment interaction obtained in this study, are different than the relation given by HUHN (1996), hence the order is as follows: van der Laan and de Kroon>KubingerHildebrand The Values of mean differences among ranks over different environments (S-i((1))) were ranged froin 2.33 to 14.83. The values of variance among ranks (Si(2)) varied from 3.33 to 154.92, while values of the relative deviations in relation to the average rank (Si 6)) ranged from 0.12 to 15.55.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - Use of non parametric statistics in estimation of genotypes stability
VL  - 54
IS  - 2-3
SP  - 155
EP  - 160
UR  - conv_741
ER  - 
@article{
author = "Delić, Nenad and Stanković, Goran and Konstantinov, Kosana",
year = "2009",
abstract = "The Mostly used, classical parametric approaches for an analysis of genotype x environment interaction are based on several assumptions: normality of: the distribution, homogeneity of variances, additivity. If some of mentioned assumptions are not fulfilled, the validity of these methods may be questionable. By use of nonparametric methods, which are simple and easy for analysis, all of the mentioned assumptions are avoided. in this paper we used several of non parametric techniques for analysis of genotype x environment interaction for grain yield of 24 maize (Zea mays Q hybrids through two locations in two years. Values of the stability measures shown that hybrids with the highest grain yield in the majority of cases were not the most stable. So as the value of the relative deviation in relation to the average rank classified the lowest yielding (4.9 t ha(-1)) genotype PR36B08 as the most stable genotype (S-i((3)) = 0.12). The interrelations of numerical values among methods, indicating the genotype x environment interaction obtained in this study, are different than the relation given by HUHN (1996), hence the order is as follows: van der Laan and de Kroon>KubingerHildebrand The Values of mean differences among ranks over different environments (S-i((1))) were ranged froin 2.33 to 14.83. The values of variance among ranks (Si(2)) varied from 3.33 to 154.92, while values of the relative deviations in relation to the average rank (Si 6)) ranged from 0.12 to 15.55.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "Use of non parametric statistics in estimation of genotypes stability",
volume = "54",
number = "2-3",
pages = "155-160",
url = "conv_741"
}
Delić, N., Stanković, G.,& Konstantinov, K.. (2009). Use of non parametric statistics in estimation of genotypes stability. in Maydica
Maydica-Ist Sper Cerealicoltur, Bergamo., 54(2-3), 155-160.
conv_741
Delić N, Stanković G, Konstantinov K. Use of non parametric statistics in estimation of genotypes stability. in Maydica. 2009;54(2-3):155-160.
conv_741 .
Delić, Nenad, Stanković, Goran, Konstantinov, Kosana, "Use of non parametric statistics in estimation of genotypes stability" in Maydica, 54, no. 2-3 (2009):155-160,
conv_741 .
7
10

Quality protein maize: QPM

Ignjatović-Micić, Dragana; Stanković, Goran; Marković, Ksenija; Lazić-Jančić, Vesna; Denić, Miloje

(Društvo genetičara Srbije, Beograd, 2008)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Marković, Ksenija
AU  - Lazić-Jančić, Vesna
AU  - Denić, Miloje
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/215
AB  - Quality protein maize (QPM) contains the opaque-2 gene along with numerous modifiers for kernel hardness. Therefore, QPM is maize with high nutritive value of endosperm protein, with substantially higher content of two essential amino acids - lysine and tryptophan, and with good agronomical performances. Although QPM was developed primarily for utilization in the regions where, because of poverty, maize is the main staple food, it has many advantages for production and consumption in other parts of the world, too. QPM can be used for production of conventional and new animal feed, as well as for human nurture. As the rate of animal weight gain is doubled with QPM and portion viability is better, a part of normal maize production could be available for other purposes, such as, for example, ethanol production. Thus, breeding QPM is set as a challenge to produce high quality protein maize with high yield and other important agronomical traits, especially with today's food and feed demands and significance of energy crisis.
AB  - Kukuruz visokog kvaliteta proteina (QPM - Quality Protein Maize) sadrži opaque-2 gen, kao i mnogobrojne modifikatore za tvrdoću zrna. QPM se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma, odnosno značajno većim sadržajem dve esencijalne aminokiseline - lizinom i triptofanom, i istovremeno dobrim agronomskim perfomansama. Mada je QPM stvoren prvenstveno za korišćenje u regionima u kojima je, zbog siromaštva, kukuruz glavna hrana, postoje mnoge prednosti za proizvodnju i korišćenje ovog kukuruza i u ostalim regionima sveta. QPM se može koristiti za proizvodnju konvencionalne i nove hrane za životinje, kao i za ishranu ljudi. Zbog dvostruko bržeg prirasta telesne težine životinja i boljeg iskorišćavanja pripremljenog obroka QPM kukuruza, deo proizvedenog standardnog kukuruza bi se mogao preusmeriti za druge potrebe, kao na primer za proizvodnju etanola. Selekcija kukuruza za poboljšanje kvaliteta proteina, zajedno sa visokim prinosom i dobrim perfomansama drugih značjnih agronomskih svojstava, predstavlja izazov za selekcionere, pogotovo imajući u vidu današnje potrebe za hranom, kao i značaj energetske krize.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Quality protein maize: QPM
T1  - Kukuruz visokog kvaliteta proteina
VL  - 40
IS  - 3
SP  - 205
EP  - 214
DO  - 10.2298/GENSR0803205I
UR  - conv_419
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Stanković, Goran and Marković, Ksenija and Lazić-Jančić, Vesna and Denić, Miloje",
year = "2008",
abstract = "Quality protein maize (QPM) contains the opaque-2 gene along with numerous modifiers for kernel hardness. Therefore, QPM is maize with high nutritive value of endosperm protein, with substantially higher content of two essential amino acids - lysine and tryptophan, and with good agronomical performances. Although QPM was developed primarily for utilization in the regions where, because of poverty, maize is the main staple food, it has many advantages for production and consumption in other parts of the world, too. QPM can be used for production of conventional and new animal feed, as well as for human nurture. As the rate of animal weight gain is doubled with QPM and portion viability is better, a part of normal maize production could be available for other purposes, such as, for example, ethanol production. Thus, breeding QPM is set as a challenge to produce high quality protein maize with high yield and other important agronomical traits, especially with today's food and feed demands and significance of energy crisis., Kukuruz visokog kvaliteta proteina (QPM - Quality Protein Maize) sadrži opaque-2 gen, kao i mnogobrojne modifikatore za tvrdoću zrna. QPM se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma, odnosno značajno većim sadržajem dve esencijalne aminokiseline - lizinom i triptofanom, i istovremeno dobrim agronomskim perfomansama. Mada je QPM stvoren prvenstveno za korišćenje u regionima u kojima je, zbog siromaštva, kukuruz glavna hrana, postoje mnoge prednosti za proizvodnju i korišćenje ovog kukuruza i u ostalim regionima sveta. QPM se može koristiti za proizvodnju konvencionalne i nove hrane za životinje, kao i za ishranu ljudi. Zbog dvostruko bržeg prirasta telesne težine životinja i boljeg iskorišćavanja pripremljenog obroka QPM kukuruza, deo proizvedenog standardnog kukuruza bi se mogao preusmeriti za druge potrebe, kao na primer za proizvodnju etanola. Selekcija kukuruza za poboljšanje kvaliteta proteina, zajedno sa visokim prinosom i dobrim perfomansama drugih značjnih agronomskih svojstava, predstavlja izazov za selekcionere, pogotovo imajući u vidu današnje potrebe za hranom, kao i značaj energetske krize.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Quality protein maize: QPM, Kukuruz visokog kvaliteta proteina",
volume = "40",
number = "3",
pages = "205-214",
doi = "10.2298/GENSR0803205I",
url = "conv_419"
}
Ignjatović-Micić, D., Stanković, G., Marković, K., Lazić-Jančić, V.,& Denić, M.. (2008). Quality protein maize: QPM. in Genetika
Društvo genetičara Srbije, Beograd., 40(3), 205-214.
https://doi.org/10.2298/GENSR0803205I
conv_419
Ignjatović-Micić D, Stanković G, Marković K, Lazić-Jančić V, Denić M. Quality protein maize: QPM. in Genetika. 2008;40(3):205-214.
doi:10.2298/GENSR0803205I
conv_419 .
Ignjatović-Micić, Dragana, Stanković, Goran, Marković, Ksenija, Lazić-Jančić, Vesna, Denić, Miloje, "Quality protein maize: QPM" in Genetika, 40, no. 3 (2008):205-214,
https://doi.org/10.2298/GENSR0803205I .,
conv_419 .
4