TY  - CONF
AU  - Božić, Manja
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1152
AB  - Small non-coding RNAs (sRNAs) have an important role in regulating plant gene expression,
both in developmental processes and stress responses, through RNA interference or silencing.
sRNAs respond to environmental stressors in manners dependant on the type of stress, tissue
and genetic background. Herein, their role in chilling stress response is examined in young maize
seedlings (Zea mays L.) of two inbred lines of contrasting chilling tolerance, using high-throughput
sequencing methods. Understanding this response is critical since one of the most promising
strategies of avoiding maize yield loss due to climate change is earlier sowing, during periods
of suboptimal temperatures. Sterilized seeds of the two lines, marked as LT (tolerant), and LS
(sensitive), were germinated in the dark for five days (optimal conditions), after which the 5-d
old seedlings were exposed to chilling conditions for 24h (temperature: 10/8 °C; photoperiod:
12/12h). Samples for RNA isolation were taken after 6h and 24h of treatment. After the cDNA
library preparations were finished, single-end 50 bp sequencing was performed (Illumina® Novaseq 6000). sRNAseq analysis was performed using a custom pipeline that included quality control, mapping, known miRNA alignment and novel miRNA prediction and quantification. This is an ongoing research and a selection of results will be presented. On average 81% of sRNA reads were succesfully mapped and belong to the class of microRNAs (miRNAs). Some of the sRNAs appear to be differentialy regulated depending on the genotype and the chilling conditions, but more research is needed to verify their potential role in this process.
PB  - Belgrade : Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - Chilling induced stress sRNAs in maize plants in early stages of development.
UR  - https://hdl.handle.net/21.15107/rcub_rik_1152
ER  - 

@conference{
author = "Božić, Manja and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2022",
abstract = "Small non-coding RNAs (sRNAs) have an important role in regulating plant gene expression,
both in developmental processes and stress responses, through RNA interference or silencing.
sRNAs respond to environmental stressors in manners dependant on the type of stress, tissue
and genetic background. Herein, their role in chilling stress response is examined in young maize
seedlings (Zea mays L.) of two inbred lines of contrasting chilling tolerance, using high-throughput
sequencing methods. Understanding this response is critical since one of the most promising
strategies of avoiding maize yield loss due to climate change is earlier sowing, during periods
of suboptimal temperatures. Sterilized seeds of the two lines, marked as LT (tolerant), and LS
(sensitive), were germinated in the dark for five days (optimal conditions), after which the 5-d
old seedlings were exposed to chilling conditions for 24h (temperature: 10/8 °C; photoperiod:
12/12h). Samples for RNA isolation were taken after 6h and 24h of treatment. After the cDNA
library preparations were finished, single-end 50 bp sequencing was performed (Illumina® Novaseq 6000). sRNAseq analysis was performed using a custom pipeline that included quality control, mapping, known miRNA alignment and novel miRNA prediction and quantification. This is an ongoing research and a selection of results will be presented. On average 81% of sRNA reads were succesfully mapped and belong to the class of microRNAs (miRNAs). Some of the sRNAs appear to be differentialy regulated depending on the genotype and the chilling conditions, but more research is needed to verify their potential role in this process.",
publisher = "Belgrade : Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "Chilling induced stress sRNAs in maize plants in early stages of development.",
url = "https://hdl.handle.net/21.15107/rcub_rik_1152"
}

Božić, M., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Nikolić, A.. (2022). Chilling induced stress sRNAs in maize plants in early stages of development.. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Belgrade : Serbian Plant Physiology Society..
https://hdl.handle.net/21.15107/rcub_rik_1152

Božić M, Ignjatović-Micić D, Vančetović J, Delić N, Nikolić A. Chilling induced stress sRNAs in maize plants in early stages of development.. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1152 .

Božić, Manja, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "Chilling induced stress sRNAs in maize plants in early stages of development." in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022),
https://hdl.handle.net/21.15107/rcub_rik_1152 .

TY  - GEN
AU  - Božić, Manja
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1139
AB  - Circular RNAs (circRNAs) are endogenous, single-stranded, covalently closed RNA molecules, generated by back-splicing events. Their role as regulators of different processes in animal cells has been widely studied and proven, however functional studies on circRNAs in plants are still few and far between. Here we attempt to bring light to their role in abiotic stress response – particularly chilling stress, in young maize seedlings (Zea mays L.) using high-throughput sequencing methods. One of the strategies of avoiding yield loss is earlier maize sowing as to escape exposure to extremely high temperatures and drought in the summer. This means submitting maize plants to suboptimal temperatures during earlier developmental stages.
Two maize inbred lines of contrasting tolerance to chilling were selected and marked as LT (tolerant), and LS (sensitive). Sterilized seeds of the two lines were germinated for five days, in the dark, in optimal conditions and after the germination period was finished, the 5-d old seedlings were exposed to chilling conditions for 24h (temperature: 10/8° C; photoperiod: 12/12h). Sampling was done after 6h and 24h of treatment. After total RNA isolation and purification, quality check, and cDNA library preparations were finished, paired-end 150 sequencing was performed (Illumina® Novaseq 6000). Custom pipeline was used for circRNAseq data analysis and included their identification and quantification (CIRCexplorer2, v2.0.5), differential expression analysis (edgeR Bioconductor v4.1) and functional enrichment (GO, KEGG, psRobot). 
Processing of this data is still underway, and by now several thousand circRNA molecules and over a thousand 5' and 3' alternative back-splicing sites have been identified. Significant number of those circRNA have shown expression dependent on chilling, as well as genotype, but further studies are necessary to confirm their role in this process, find their target molecules and unravel regulatory networks they create with other types of RNAs.
T2  - 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts
T1  - circRNAs involved in chilling stress response during early developmental stages in maize
UR  - https://hdl.handle.net/21.15107/rcub_rik_1139
ER  - 

@misc{
author = "Božić, Manja and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2022",
abstract = "Circular RNAs (circRNAs) are endogenous, single-stranded, covalently closed RNA molecules, generated by back-splicing events. Their role as regulators of different processes in animal cells has been widely studied and proven, however functional studies on circRNAs in plants are still few and far between. Here we attempt to bring light to their role in abiotic stress response – particularly chilling stress, in young maize seedlings (Zea mays L.) using high-throughput sequencing methods. One of the strategies of avoiding yield loss is earlier maize sowing as to escape exposure to extremely high temperatures and drought in the summer. This means submitting maize plants to suboptimal temperatures during earlier developmental stages.
Two maize inbred lines of contrasting tolerance to chilling were selected and marked as LT (tolerant), and LS (sensitive). Sterilized seeds of the two lines were germinated for five days, in the dark, in optimal conditions and after the germination period was finished, the 5-d old seedlings were exposed to chilling conditions for 24h (temperature: 10/8° C; photoperiod: 12/12h). Sampling was done after 6h and 24h of treatment. After total RNA isolation and purification, quality check, and cDNA library preparations were finished, paired-end 150 sequencing was performed (Illumina® Novaseq 6000). Custom pipeline was used for circRNAseq data analysis and included their identification and quantification (CIRCexplorer2, v2.0.5), differential expression analysis (edgeR Bioconductor v4.1) and functional enrichment (GO, KEGG, psRobot). 
Processing of this data is still underway, and by now several thousand circRNA molecules and over a thousand 5' and 3' alternative back-splicing sites have been identified. Significant number of those circRNA have shown expression dependent on chilling, as well as genotype, but further studies are necessary to confirm their role in this process, find their target molecules and unravel regulatory networks they create with other types of RNAs.",
journal = "25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts",
title = "circRNAs involved in chilling stress response during early developmental stages in maize",
url = "https://hdl.handle.net/21.15107/rcub_rik_1139"
}

Božić, M., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Nikolić, A.. (2022). circRNAs involved in chilling stress response during early developmental stages in maize. in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts.
https://hdl.handle.net/21.15107/rcub_rik_1139

Božić M, Ignjatović-Micić D, Vančetović J, Delić N, Nikolić A. circRNAs involved in chilling stress response during early developmental stages in maize. in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1139 .

Božić, Manja, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "circRNAs involved in chilling stress response during early developmental stages in maize" in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_rik_1139 .

TY  - PAT
AU  - Božinović, Sofija
AU  - Vančetović, Jelena
AU  - Grčić, Nikola
AU  - Nikolić, Ana
AU  - Đorđević Melnik, Olivera
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1102
T2  - Rešenje:320-44-02159/2/2020-11
T1  - ZP5023
UR  - https://hdl.handle.net/21.15107/rcub_rik_1102
ER  - 

@misc{
author = "Božinović, Sofija and Vančetović, Jelena and Grčić, Nikola and Nikolić, Ana and Đorđević Melnik, Olivera",
year = "2022",
journal = "Rešenje:320-44-02159/2/2020-11",
title = "ZP5023",
url = "https://hdl.handle.net/21.15107/rcub_rik_1102"
}

Božinović, S., Vančetović, J., Grčić, N., Nikolić, A.,& Đorđević Melnik, O.. (2022). ZP5023. in Rešenje:320-44-02159/2/2020-11.
https://hdl.handle.net/21.15107/rcub_rik_1102

Božinović S, Vančetović J, Grčić N, Nikolić A, Đorđević Melnik O. ZP5023. in Rešenje:320-44-02159/2/2020-11. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1102 .

Božinović, Sofija, Vančetović, Jelena, Grčić, Nikola, Nikolić, Ana, Đorđević Melnik, Olivera, "ZP5023" in Rešenje:320-44-02159/2/2020-11 (2022),
https://hdl.handle.net/21.15107/rcub_rik_1102 .

TY  - CONF
AU  - Mladenović Drinić, Snežana
AU  - Srdić, Jelena
AU  - Anđelković, Violeta
AU  - Grčić, Nikola
AU  - Kravić, Natalija
AU  - Filipović, Milomir
AU  - Nikolić, Ana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/958
AB  - Popcorn is a type of specialty maize and plays important role in the history and the spread of
this species. This type of maize historicaly received little atention in genetic diversity studies
while the fact is that information on genetic variability and population structure is of crucial
importance for improvement of breeding programs. The genetic variation of popcorn maize
was mainly analysed using SSR markers, but there are not many studies applying high-density
SNP markers with lower genotyping errors. The aim of this study was the assesment of genetic
diversity and population structure of popcorn germplasm which represents the genetic
resources currently used in breeding programs in Maize Research Institute „Zemun Polje“.
Molecular characterization of 44 popcorn genotypes was done using 25k SNP Illumina Infinum
Array for maize. After quality control filtering, 12335 SNPs were selected for downstream
analyses. Genetic distance detected ranged from 0.001 to 0.51 with an average of 0.33. The
majority of genetic distances (70.6%) fell between 0.30 and 0.51 revealing significant genetic
variability in a set of maize genotypes under study. SNP markes were moderatly informative
with an average PIC value of 0.26. Also, for more than 50% of SNPs, PIC value was greater
than 0.30. The average observed heterozygosity was 0.04 ranging from 0 to 0.11. The
clustering, principal component and model-based STRUCTURE analyses showed similar
grouping of popcorn lines, generally in accordance with their genetic background. The results
of this research gave valuable information for initiation of future breeding strategies.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Molecular characterization of popcorn inbreds using 25k SNP array
UR  - https://hdl.handle.net/21.15107/rcub_rik_958
ER  - 

@conference{
author = "Mladenović Drinić, Snežana and Srdić, Jelena and Anđelković, Violeta and Grčić, Nikola and Kravić, Natalija and Filipović, Milomir and Nikolić, Ana",
year = "2022",
abstract = "Popcorn is a type of specialty maize and plays important role in the history and the spread of
this species. This type of maize historicaly received little atention in genetic diversity studies
while the fact is that information on genetic variability and population structure is of crucial
importance for improvement of breeding programs. The genetic variation of popcorn maize
was mainly analysed using SSR markers, but there are not many studies applying high-density
SNP markers with lower genotyping errors. The aim of this study was the assesment of genetic
diversity and population structure of popcorn germplasm which represents the genetic
resources currently used in breeding programs in Maize Research Institute „Zemun Polje“.
Molecular characterization of 44 popcorn genotypes was done using 25k SNP Illumina Infinum
Array for maize. After quality control filtering, 12335 SNPs were selected for downstream
analyses. Genetic distance detected ranged from 0.001 to 0.51 with an average of 0.33. The
majority of genetic distances (70.6%) fell between 0.30 and 0.51 revealing significant genetic
variability in a set of maize genotypes under study. SNP markes were moderatly informative
with an average PIC value of 0.26. Also, for more than 50% of SNPs, PIC value was greater
than 0.30. The average observed heterozygosity was 0.04 ranging from 0 to 0.11. The
clustering, principal component and model-based STRUCTURE analyses showed similar
grouping of popcorn lines, generally in accordance with their genetic background. The results
of this research gave valuable information for initiation of future breeding strategies.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Molecular characterization of popcorn inbreds using 25k SNP array",
url = "https://hdl.handle.net/21.15107/rcub_rik_958"
}

Mladenović Drinić, S., Srdić, J., Anđelković, V., Grčić, N., Kravić, N., Filipović, M.,& Nikolić, A.. (2022). Molecular characterization of popcorn inbreds using 25k SNP array. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje..
https://hdl.handle.net/21.15107/rcub_rik_958

Mladenović Drinić S, Srdić J, Anđelković V, Grčić N, Kravić N, Filipović M, Nikolić A. Molecular characterization of popcorn inbreds using 25k SNP array. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_958 .

Mladenović Drinić, Snežana, Srdić, Jelena, Anđelković, Violeta, Grčić, Nikola, Kravić, Natalija, Filipović, Milomir, Nikolić, Ana, "Molecular characterization of popcorn inbreds using 25k SNP array" in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_rik_958 .

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Nikolić, Ana
AU  - Jauković, Marko
AU  - Kandić, Vesna
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/836
AB  - In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Toxins
T1  - Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia
VL  - 13
SP  - 847
DO  - 10.3390/toxins13120847
ER  - 

@article{
author = "Nikolić, Milica and Savić, Iva and Nikolić, Ana and Jauković, Marko and Kandić, Vesna and Stevanović, Milan and Stanković, Slavica",
year = "2021",
abstract = "In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Toxins",
title = "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia",
volume = "13",
pages = "847",
doi = "10.3390/toxins13120847"
}

Nikolić, M., Savić, I., Nikolić, A., Jauković, M., Kandić, V., Stevanović, M.,& Stanković, S.. (2021). Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 13, 847.
https://doi.org/10.3390/toxins13120847

Nikolić M, Savić I, Nikolić A, Jauković M, Kandić V, Stevanović M, Stanković S. Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins. 2021;13:847.
doi:10.3390/toxins13120847 .

Nikolić, Milica, Savić, Iva, Nikolić, Ana, Jauković, Marko, Kandić, Vesna, Stevanović, Milan, Stanković, Slavica, "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia" in Toxins, 13 (2021):847,
https://doi.org/10.3390/toxins13120847 . .

TY  - CONF
AU  - Božić, Manja
AU  - Nikolić, Ana
AU  - Dudić, Dragana
AU  - Ignjatović-Micić, Dragana
AU  - Samardžić, Jelena
AU  - Delić, Nenad
AU  - Banović Đeri, Bojana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1147
AB  - Finding new ways of improving crop quality, yield potential and abiotic stress tolerance are some of the most important pursuits in crop production today. As one of the biggest causes of yield and productivity reduction is climate change, specifically increasing temperatures and drought during the summer, a large number of strategies is focussed on lessening their negative effects. Cropping pattern changes include earlier sowing (early spring), when the temperatures are lower, as one of the most promising escape strategies for avoiding high summer temperatures. Thus, development of cold tolerant maize lines became an important goal.
Comparative analysis of 46 maize inbred lines belonging to two different genetic backgrounds, one predominantly cold tolerante (marked as Non-Lancaster) and the other predominantly cold sensitive (marked as Lancaster) in the field, was done by whole transriptome sequencing and differential gene expression (DGE) analysis.  Plants were grown under optimal, greenhouse conditions and sampled after completing the V4 growth stage. Total RNA isolated from leaves of three plants per inbred line was used for cDNA library preparation by Illumina TruSeq Stranded RNA LT kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent kit, v2 (2 x 150bp). Data manipulation and analysis was performed using a custom-made bioinformatics pipeline that included high throughput sequence data quality control (using FastQC), removal of low quality reads (using Trimmomatic tool, version 0.32), transcriptome assembly and mapping (using Cufflinks, version 2.2.1), expression quantification (using CuffDiff) and DGE analysis (using BLAST2GO and GO analysis Toolkit and Database for Agricultural Community, agriGO v2).  
DGE analysis revealed 77 differentially expressed genes (DEGs) between the Lancaster and the Non-Lancaster group, 21 of which were  statistically supported for differential expression between the two groups and annotated as involved in abiotic stress responses in maize and other plant species. To test DEGs response to cold stress expression of a subset of seven DEGs in eight inbred lines (4 belonging to Lancaster and 4 belonging to Non-Lancaster genetic background) was analyzed under 24h long exposure to low temperatures (6/4° C, 12h photoperiod), with sampling being done 6h and 24h after beginning of the treatment, as well as after 48h of recovery. Six DEGs showed different expression regulation dependent on cold exposure duration and genetic background.  These findings imply differently regulated processes between the analysed Lancaster and Non-Lancaster inbred lines, contributing to their different cold response and adaptation, and will be further used for the development of cold tolerant hybrids.
PB  - Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts
T1  - Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_rik_1147
ER  - 

@conference{
author = "Božić, Manja and Nikolić, Ana and Dudić, Dragana and Ignjatović-Micić, Dragana and Samardžić, Jelena and Delić, Nenad and Banović Đeri, Bojana",
year = "2021",
abstract = "Finding new ways of improving crop quality, yield potential and abiotic stress tolerance are some of the most important pursuits in crop production today. As one of the biggest causes of yield and productivity reduction is climate change, specifically increasing temperatures and drought during the summer, a large number of strategies is focussed on lessening their negative effects. Cropping pattern changes include earlier sowing (early spring), when the temperatures are lower, as one of the most promising escape strategies for avoiding high summer temperatures. Thus, development of cold tolerant maize lines became an important goal.
Comparative analysis of 46 maize inbred lines belonging to two different genetic backgrounds, one predominantly cold tolerante (marked as Non-Lancaster) and the other predominantly cold sensitive (marked as Lancaster) in the field, was done by whole transriptome sequencing and differential gene expression (DGE) analysis.  Plants were grown under optimal, greenhouse conditions and sampled after completing the V4 growth stage. Total RNA isolated from leaves of three plants per inbred line was used for cDNA library preparation by Illumina TruSeq Stranded RNA LT kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent kit, v2 (2 x 150bp). Data manipulation and analysis was performed using a custom-made bioinformatics pipeline that included high throughput sequence data quality control (using FastQC), removal of low quality reads (using Trimmomatic tool, version 0.32), transcriptome assembly and mapping (using Cufflinks, version 2.2.1), expression quantification (using CuffDiff) and DGE analysis (using BLAST2GO and GO analysis Toolkit and Database for Agricultural Community, agriGO v2).  
DGE analysis revealed 77 differentially expressed genes (DEGs) between the Lancaster and the Non-Lancaster group, 21 of which were  statistically supported for differential expression between the two groups and annotated as involved in abiotic stress responses in maize and other plant species. To test DEGs response to cold stress expression of a subset of seven DEGs in eight inbred lines (4 belonging to Lancaster and 4 belonging to Non-Lancaster genetic background) was analyzed under 24h long exposure to low temperatures (6/4° C, 12h photoperiod), with sampling being done 6h and 24h after beginning of the treatment, as well as after 48h of recovery. Six DEGs showed different expression regulation dependent on cold exposure duration and genetic background.  These findings imply differently regulated processes between the analysed Lancaster and Non-Lancaster inbred lines, contributing to their different cold response and adaptation, and will be further used for the development of cold tolerant hybrids.",
publisher = "Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts",
title = "Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_rik_1147"
}

Božić, M., Nikolić, A., Dudić, D., Ignjatović-Micić, D., Samardžić, J., Delić, N.,& Banović Đeri, B.. (2021). Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature. in Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts
Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43.
https://hdl.handle.net/21.15107/rcub_rik_1147

Božić M, Nikolić A, Dudić D, Ignjatović-Micić D, Samardžić J, Delić N, Banović Đeri B. Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature. in Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts. 2021;43.
https://hdl.handle.net/21.15107/rcub_rik_1147 .

Božić, Manja, Nikolić, Ana, Dudić, Dragana, Ignjatović-Micić, Dragana, Samardžić, Jelena, Delić, Nenad, Banović Đeri, Bojana, "Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature" in Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts, 43 (2021),
https://hdl.handle.net/21.15107/rcub_rik_1147 .

TY  - CONF
AU  - Božić, Manja
AU  - Nikolić, Ana
AU  - Dudić, Dragana
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Banović Đeri, Bojana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1144
AB  - Improving yield potential, crop quality and abiotic stress tolerance have always been some of the most important requirements for successful crop production. Poor environmental conditions arising as repercussions of climate change, like the drastically higher temperatures and drought during the summer, have become a significant cause of productivity and yield loss. Therefore, many strategies are focused on minimizing their negative effects, like changing the cropping patterns, including earlier sowing (early spring). Maize is especially sensitive to extreme heat occurring during the flowering and grain filling stages in summer, and earlier sowing enables avoiding this. However, it also means exposure to suboptimal temperatures and chilling stress during earlier developmental stages, leading to a demand for the development of maize lines tolerant to low temperatures during those stages.
In this study, 46 maize lines used in breeding programmes were grouped as Lancaster and group consisting of different heterotic groups like BSSS, Iowa dent, etc. and further studied by whole transcriptome sequencing (maize leaves, V4 stage, optimal temperature conditions). Gene expression analyses revealed a set of 77 differentially expressed genes (DEGs) between the two groups, out of which 20 were annotated as related to abiotic stress response. ATP synthase CF0 A subunit gene (atpI) was chosen for further characterization under low temperature conditions in two inbred lines (L1, L2) with most contrasting FPKM values, one belonging to each group. The experiment was performed with 5-day old and V4 maize seedlings, under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. 
The results showed different expression regulation of atpI dependent on cold exposure duration, developmental stage and genetic background. atpI expression was up-regulated in both genotypes in V4 stage, with the expression peak after 6h of treatment. In the 5-day old seedling stage, atpI expression depended on the genotype – it was down-regulated in L1, and up-regulated in L2. The expression in both genotypes in this developmental stage was at its highest after 24h of treatment. This suggests that mechanisms involved in ATP synthesis and photosynthetic phosphorylation are differentially regulated based on low temperature exposure duration, developmental stage and genetic background.
PB  - Magnus Group (MG), USA
C3  - 6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts
T1  - atpI expression in different developmental stages of maize during chilling
UR  - https://hdl.handle.net/21.15107/rcub_rik_1144
ER  - 

@conference{
author = "Božić, Manja and Nikolić, Ana and Dudić, Dragana and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Banović Đeri, Bojana",
year = "2021",
abstract = "Improving yield potential, crop quality and abiotic stress tolerance have always been some of the most important requirements for successful crop production. Poor environmental conditions arising as repercussions of climate change, like the drastically higher temperatures and drought during the summer, have become a significant cause of productivity and yield loss. Therefore, many strategies are focused on minimizing their negative effects, like changing the cropping patterns, including earlier sowing (early spring). Maize is especially sensitive to extreme heat occurring during the flowering and grain filling stages in summer, and earlier sowing enables avoiding this. However, it also means exposure to suboptimal temperatures and chilling stress during earlier developmental stages, leading to a demand for the development of maize lines tolerant to low temperatures during those stages.
In this study, 46 maize lines used in breeding programmes were grouped as Lancaster and group consisting of different heterotic groups like BSSS, Iowa dent, etc. and further studied by whole transcriptome sequencing (maize leaves, V4 stage, optimal temperature conditions). Gene expression analyses revealed a set of 77 differentially expressed genes (DEGs) between the two groups, out of which 20 were annotated as related to abiotic stress response. ATP synthase CF0 A subunit gene (atpI) was chosen for further characterization under low temperature conditions in two inbred lines (L1, L2) with most contrasting FPKM values, one belonging to each group. The experiment was performed with 5-day old and V4 maize seedlings, under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. 
The results showed different expression regulation of atpI dependent on cold exposure duration, developmental stage and genetic background. atpI expression was up-regulated in both genotypes in V4 stage, with the expression peak after 6h of treatment. In the 5-day old seedling stage, atpI expression depended on the genotype – it was down-regulated in L1, and up-regulated in L2. The expression in both genotypes in this developmental stage was at its highest after 24h of treatment. This suggests that mechanisms involved in ATP synthesis and photosynthetic phosphorylation are differentially regulated based on low temperature exposure duration, developmental stage and genetic background.",
publisher = "Magnus Group (MG), USA",
journal = "6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts",
title = "atpI expression in different developmental stages of maize during chilling",
url = "https://hdl.handle.net/21.15107/rcub_rik_1144"
}

Božić, M., Nikolić, A., Dudić, D., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Banović Đeri, B.. (2021). atpI expression in different developmental stages of maize during chilling. in 6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts
Magnus Group (MG), USA..
https://hdl.handle.net/21.15107/rcub_rik_1144

Božić M, Nikolić A, Dudić D, Ignjatović-Micić D, Vančetović J, Delić N, Banović Đeri B. atpI expression in different developmental stages of maize during chilling. in 6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1144 .

Božić, Manja, Nikolić, Ana, Dudić, Dragana, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Banović Đeri, Bojana, "atpI expression in different developmental stages of maize during chilling" in 6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts (2021),
https://hdl.handle.net/21.15107/rcub_rik_1144 .

TY  - CONF
AU  - Božić, Manja
AU  - Banović Đeri, Bojana
AU  - Dudić, Dragana
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1146
AB  - Earlier sowing is one of the most important strategies of ensuring good yield potential and crop quality under poor environmental conditions arising as consequences of climate changes. Sowing maize in early spring, when the temperatures are lower, enables avoidance of drought and high summer temperatures during the flowering and grain filling stages, but it also means that maize plants will be exposed to suboptimal temperatures during earlier developmental stages. Consequently, development of maize lines tolerant to low temperatures during those stages becomes precedence. 
An initial study encompassed whole transcriptome sequencing of 46 maize inbred lines at V4 stage grown under optimal temperature conditions. Gene expression analysis of maize genotypes grouped as Lancaster and non-Lancaster (BSSS, Iowa dent, etc.) revealed a set of 77 differentially expressed genes (DEGs). Seven of these genes, related to abiotic stress response, were further characterized under low temperature conditions in eight inbred lines at V4 stage. Their expression showed specific profiles depending on the duration of low temperature exposure and genetic background. To test if these genes follow the same expression patterns in earlier developmental stages, the experiment was performed with 5-day old maize seedlings of two inbred lines (tolerant and sensitive), under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. Five analyzed genes showed different expression regulation dependent on cold exposure duration. Two genes showed regulation dependent both on cold exposure duration and genetic background. Additionally, three of five DEGs showed different expression patterns at 5-day old seedling stage than at V4 stage. The results imply that processes underlying maize low temperature response are differentially regulated based on stress exposure duration, developmental stage and genetic background.
PB  - Belgrade : Maize Research Institute "Zemun Polje"
C3  - International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts
T1  - Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages
UR  - https://hdl.handle.net/21.15107/rcub_rik_1146
ER  - 

@conference{
author = "Božić, Manja and Banović Đeri, Bojana and Dudić, Dragana and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2021",
abstract = "Earlier sowing is one of the most important strategies of ensuring good yield potential and crop quality under poor environmental conditions arising as consequences of climate changes. Sowing maize in early spring, when the temperatures are lower, enables avoidance of drought and high summer temperatures during the flowering and grain filling stages, but it also means that maize plants will be exposed to suboptimal temperatures during earlier developmental stages. Consequently, development of maize lines tolerant to low temperatures during those stages becomes precedence. 
An initial study encompassed whole transcriptome sequencing of 46 maize inbred lines at V4 stage grown under optimal temperature conditions. Gene expression analysis of maize genotypes grouped as Lancaster and non-Lancaster (BSSS, Iowa dent, etc.) revealed a set of 77 differentially expressed genes (DEGs). Seven of these genes, related to abiotic stress response, were further characterized under low temperature conditions in eight inbred lines at V4 stage. Their expression showed specific profiles depending on the duration of low temperature exposure and genetic background. To test if these genes follow the same expression patterns in earlier developmental stages, the experiment was performed with 5-day old maize seedlings of two inbred lines (tolerant and sensitive), under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. Five analyzed genes showed different expression regulation dependent on cold exposure duration. Two genes showed regulation dependent both on cold exposure duration and genetic background. Additionally, three of five DEGs showed different expression patterns at 5-day old seedling stage than at V4 stage. The results imply that processes underlying maize low temperature response are differentially regulated based on stress exposure duration, developmental stage and genetic background.",
publisher = "Belgrade : Maize Research Institute "Zemun Polje"",
journal = "International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts",
title = "Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages",
url = "https://hdl.handle.net/21.15107/rcub_rik_1146"
}

Božić, M., Banović Đeri, B., Dudić, D., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Nikolić, A.. (2021). Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages. in International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts
Belgrade : Maize Research Institute "Zemun Polje"..
https://hdl.handle.net/21.15107/rcub_rik_1146

Božić M, Banović Đeri B, Dudić D, Ignjatović-Micić D, Vančetović J, Delić N, Nikolić A. Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages. in International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1146 .

Božić, Manja, Banović Đeri, Bojana, Dudić, Dragana, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages" in International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts (2021),
https://hdl.handle.net/21.15107/rcub_rik_1146 .

TY  - GEN
AU  - Nikolić, Ana
AU  - Banović Đeri, Bojana
AU  - Dudić, Dragana
AU  - Božić, Manja
AU  - Marković, Ksenija
AU  - Delić, Nenad
AU  - Ignjatović-Micić, Dragana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1138
AB  - Abiotic stress, as an adverse environmental condition, seriously affects and limits 
maize productivity worldwide. Climate changes lead to altering temperatures and 
rainfall patterns. Earlier maize sowing enables avoiding high temperatures during 
silking and pollination season. As a consequence of early sowing, damages 
caused by temperatures lower than optimal can occur. Hence, it is very important 
to know the mechanism of defence against the low temperatures in the early 
developmental stages of the plant. Nowadays, thanks to hightroughput methods 
such as Next Generation Sequencing (NGS), more profound and detailed research 
can be conducted. A set of maize inbred lines important for commercial breeding 
programs at Maize Research Institute, Zemun Polje was analysed by sequencing 
the whole transcriptome. The aim was to identify tolerant lines for planning future 
hybrids, try to unravel mechanism involved in maize response to low 
temperatures and to identify molecular markers that could be included in breeding 
programs. Bioinformatic analysis of Single Nucleotide Polymorphisms (SNPs) 
and Diferentially Expressed Genes (DEGs) was done. Cold-induced expression 
analysis of several DEGs revealed different expression regulation dependent on 
the duration of cold stress signalizing possible important role of these genes in 
maize response to low temperatures. A more profound transcriptome analysis, 
expected to give a comprehensive insight into low-temperature induced response 
in early stages of plant development, is already underway. Besides low temperatures, waterlogging caused by more frequent rainfalls is another abiotic 
stress which can hamper the idea of early sowing, especially if combined with 
low-temperatures. For this reason, plant response to this stress will also be studied 
on the same levels as low-temperature stress, with the final goal to develop 
hybrids which can respond to challenging climate changes.
PB  - Belgrade-Zemun : Maize Research Institute, Zemun Polje
T2  - International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts
T1  - Understanding low-temperature and waterlogging stress impact on early stages of maize plant development.
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_rik_1138
ER  - 

@misc{
author = "Nikolić, Ana and Banović Đeri, Bojana and Dudić, Dragana and Božić, Manja and Marković, Ksenija and Delić, Nenad and Ignjatović-Micić, Dragana",
year = "2021",
abstract = "Abiotic stress, as an adverse environmental condition, seriously affects and limits 
maize productivity worldwide. Climate changes lead to altering temperatures and 
rainfall patterns. Earlier maize sowing enables avoiding high temperatures during 
silking and pollination season. As a consequence of early sowing, damages 
caused by temperatures lower than optimal can occur. Hence, it is very important 
to know the mechanism of defence against the low temperatures in the early 
developmental stages of the plant. Nowadays, thanks to hightroughput methods 
such as Next Generation Sequencing (NGS), more profound and detailed research 
can be conducted. A set of maize inbred lines important for commercial breeding 
programs at Maize Research Institute, Zemun Polje was analysed by sequencing 
the whole transcriptome. The aim was to identify tolerant lines for planning future 
hybrids, try to unravel mechanism involved in maize response to low 
temperatures and to identify molecular markers that could be included in breeding 
programs. Bioinformatic analysis of Single Nucleotide Polymorphisms (SNPs) 
and Diferentially Expressed Genes (DEGs) was done. Cold-induced expression 
analysis of several DEGs revealed different expression regulation dependent on 
the duration of cold stress signalizing possible important role of these genes in 
maize response to low temperatures. A more profound transcriptome analysis, 
expected to give a comprehensive insight into low-temperature induced response 
in early stages of plant development, is already underway. Besides low temperatures, waterlogging caused by more frequent rainfalls is another abiotic 
stress which can hamper the idea of early sowing, especially if combined with 
low-temperatures. For this reason, plant response to this stress will also be studied 
on the same levels as low-temperature stress, with the final goal to develop 
hybrids which can respond to challenging climate changes.",
publisher = "Belgrade-Zemun : Maize Research Institute, Zemun Polje",
journal = "International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts",
title = "Understanding low-temperature and waterlogging stress impact on early stages of maize plant development.",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_rik_1138"
}

Nikolić, A., Banović Đeri, B., Dudić, D., Božić, M., Marković, K., Delić, N.,& Ignjatović-Micić, D.. (2021). Understanding low-temperature and waterlogging stress impact on early stages of maize plant development.. in International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts
Belgrade-Zemun : Maize Research Institute, Zemun Polje., 50.
https://hdl.handle.net/21.15107/rcub_rik_1138

Nikolić A, Banović Đeri B, Dudić D, Božić M, Marković K, Delić N, Ignjatović-Micić D. Understanding low-temperature and waterlogging stress impact on early stages of maize plant development.. in International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts. 2021;:50.
https://hdl.handle.net/21.15107/rcub_rik_1138 .

Nikolić, Ana, Banović Đeri, Bojana, Dudić, Dragana, Božić, Manja, Marković, Ksenija, Delić, Nenad, Ignjatović-Micić, Dragana, "Understanding low-temperature and waterlogging stress impact on early stages of maize plant development." in International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts (2021):50,
https://hdl.handle.net/21.15107/rcub_rik_1138 .

TY  - JOUR
AU  - Banović Đeri, Bojana
AU  - Božić, Manja
AU  - Dudić, Dragana
AU  - Vićić, Ivan
AU  - Milivojević, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Samardžić, Jelena
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1137
AB  - One of the strategies for overcoming global climate change threatening to decrease maize yield is early sowing. To contribute to the development of cold-tolerant hybrids this research focused on the genetic background's comparative analysis in maize inbreds with good combining ability. Leaf whole-transcriptome sequencing of 46 maize genotypes revealed 77 differentially expressed genes (DEGs) between Lancaster and other heterotic groups (i.e. BSSS, Iowa dent, Ohio), referred to as non-Lancaster group, under optimal growing conditions. Cold test of the subset of four Lancaster and four non-Lancaster lines showed that the former were cold sensitive and the latter cold tolerant. Cold-induced expression analysis of seven DEGs in eight lines revealed different expression regulation dependent on the duration of cold exposure and genetic background for six out of seven analysed genes—chloroplast ATP-sulphurylase, photosystem II cytochrome b559 alpha subunit, CIPK serine-threonine protein kinase 15, glutamyl-tRNA reductase, photosystem II reaction centre protein I and Calvin cycle CP12-chloroplastic-like encoding genes. The results imply that differently regulated basic processes between Lancaster and non-Lancaster maize group involve, at least, photosynthesis and sulphate assimilation, contributing to their different cold response and different adaptation to low temperatures.
PB  - Hoboken, New Jersey : Wiley
T2  - Journal of Agronomy and Crop Science
T1  - Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes
VL  - 208
SP  - 12529
DO  - 10.1111/jac.12529
ER  - 

@article{
author = "Banović Đeri, Bojana and Božić, Manja and Dudić, Dragana and Vićić, Ivan and Milivojević, Marija and Ignjatović-Micić, Dragana and Samardžić, Jelena and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2021",
abstract = "One of the strategies for overcoming global climate change threatening to decrease maize yield is early sowing. To contribute to the development of cold-tolerant hybrids this research focused on the genetic background's comparative analysis in maize inbreds with good combining ability. Leaf whole-transcriptome sequencing of 46 maize genotypes revealed 77 differentially expressed genes (DEGs) between Lancaster and other heterotic groups (i.e. BSSS, Iowa dent, Ohio), referred to as non-Lancaster group, under optimal growing conditions. Cold test of the subset of four Lancaster and four non-Lancaster lines showed that the former were cold sensitive and the latter cold tolerant. Cold-induced expression analysis of seven DEGs in eight lines revealed different expression regulation dependent on the duration of cold exposure and genetic background for six out of seven analysed genes—chloroplast ATP-sulphurylase, photosystem II cytochrome b559 alpha subunit, CIPK serine-threonine protein kinase 15, glutamyl-tRNA reductase, photosystem II reaction centre protein I and Calvin cycle CP12-chloroplastic-like encoding genes. The results imply that differently regulated basic processes between Lancaster and non-Lancaster maize group involve, at least, photosynthesis and sulphate assimilation, contributing to their different cold response and different adaptation to low temperatures.",
publisher = "Hoboken, New Jersey : Wiley",
journal = "Journal of Agronomy and Crop Science",
title = "Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes",
volume = "208",
pages = "12529",
doi = "10.1111/jac.12529"
}

Banović Đeri, B., Božić, M., Dudić, D., Vićić, I., Milivojević, M., Ignjatović-Micić, D., Samardžić, J., Vančetović, J., Delić, N.,& Nikolić, A.. (2021). Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes. in Journal of Agronomy and Crop Science
Hoboken, New Jersey : Wiley., 208, 12529.
https://doi.org/10.1111/jac.12529

Banović Đeri B, Božić M, Dudić D, Vićić I, Milivojević M, Ignjatović-Micić D, Samardžić J, Vančetović J, Delić N, Nikolić A. Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes. in Journal of Agronomy and Crop Science. 2021;208:12529.
doi:10.1111/jac.12529 .

Banović Đeri, Bojana, Božić, Manja, Dudić, Dragana, Vićić, Ivan, Milivojević, Marija, Ignjatović-Micić, Dragana, Samardžić, Jelena, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes" in Journal of Agronomy and Crop Science, 208 (2021):12529,
https://doi.org/10.1111/jac.12529 . .

TY  - PAT
AU  - Grčić, Nikola
AU  - Filipović, Milomir
AU  - Nikolić, Ana
AU  - Mladenović, Marko
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1083
T2  - Rešenje:320-04-02261/2/2019-11
T1  - ZP6091
UR  - https://hdl.handle.net/21.15107/rcub_rik_1083
ER  - 

@misc{
author = "Grčić, Nikola and Filipović, Milomir and Nikolić, Ana and Mladenović, Marko",
year = "2021",
journal = "Rešenje:320-04-02261/2/2019-11",
title = "ZP6091",
url = "https://hdl.handle.net/21.15107/rcub_rik_1083"
}

Grčić, N., Filipović, M., Nikolić, A.,& Mladenović, M.. (2021). ZP6091. in Rešenje:320-04-02261/2/2019-11.
https://hdl.handle.net/21.15107/rcub_rik_1083

Grčić N, Filipović M, Nikolić A, Mladenović M. ZP6091. in Rešenje:320-04-02261/2/2019-11. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1083 .

Grčić, Nikola, Filipović, Milomir, Nikolić, Ana, Mladenović, Marko, "ZP6091" in Rešenje:320-04-02261/2/2019-11 (2021),
https://hdl.handle.net/21.15107/rcub_rik_1083 .

TY  - JOUR
AU  - Božić, Manja
AU  - Stanojević, Aleksandra
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Dragana
AU  - Milivojević, Marija
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/832
AB  - Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance.
AB  - Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions
VL  - 53
IS  - 3
SP  - 1147
EP  - 1165
DO  - 10.2298/GENSR2103147B
ER  - 

@article{
author = "Božić, Manja and Stanojević, Aleksandra and Marković, Ksenija and Ignjatović-Micić, Dragana and Nikolić, Dragana and Milivojević, Marija and Nikolić, Ana",
year = "2021",
abstract = "Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance., Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions",
volume = "53",
number = "3",
pages = "1147-1165",
doi = "10.2298/GENSR2103147B"
}

Božić, M., Stanojević, A., Marković, K., Ignjatović-Micić, D., Nikolić, D., Milivojević, M.,& Nikolić, A.. (2021). Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika
Belgrade : Serbian Genetics Society., 53(3), 1147-1165.
https://doi.org/10.2298/GENSR2103147B

Božić M, Stanojević A, Marković K, Ignjatović-Micić D, Nikolić D, Milivojević M, Nikolić A. Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika. 2021;53(3):1147-1165.
doi:10.2298/GENSR2103147B .

Božić, Manja, Stanojević, Aleksandra, Marković, Ksenija, Ignjatović-Micić, Dragana, Nikolić, Dragana, Milivojević, Marija, Nikolić, Ana, "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions" in Genetika, 53, no. 3 (2021):1147-1165,
https://doi.org/10.2298/GENSR2103147B . .

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kravić, Natalija
AU  - Ristić, Danijela
AU  - Anđelković, Violeta
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/814
AB  - Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing.
AB  - Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve
PB  - Beograd : Društavo genetičara Srbije
T2  - Genetika
T1  - Morphological and physiological response of maize seedlings to chilling stress
VL  - 52
IS  - 2
SP  - 689
EP  - 698
DO  - 10.2298/GENSR2002689N
ER  - 

@article{
author = "Nikolić, Ana and Kravić, Natalija and Ristić, Danijela and Anđelković, Violeta and Marković, Ksenija and Vančetović, Jelena and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing., Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve",
publisher = "Beograd : Društavo genetičara Srbije",
journal = "Genetika",
title = "Morphological and physiological response of maize seedlings to chilling stress",
volume = "52",
number = "2",
pages = "689-698",
doi = "10.2298/GENSR2002689N"
}

Nikolić, A., Kravić, N., Ristić, D., Anđelković, V., Marković, K., Vančetović, J.,& Ignjatović-Micić, D.. (2020). Morphological and physiological response of maize seedlings to chilling stress. in Genetika
Beograd : Društavo genetičara Srbije., 52(2), 689-698.
https://doi.org/10.2298/GENSR2002689N

Nikolić A, Kravić N, Ristić D, Anđelković V, Marković K, Vančetović J, Ignjatović-Micić D. Morphological and physiological response of maize seedlings to chilling stress. in Genetika. 2020;52(2):689-698.
doi:10.2298/GENSR2002689N .

Nikolić, Ana, Kravić, Natalija, Ristić, Danijela, Anđelković, Violeta, Marković, Ksenija, Vančetović, Jelena, Ignjatović-Micić, Dragana, "Morphological and physiological response of maize seedlings to chilling stress" in Genetika, 52, no. 2 (2020):689-698,
https://doi.org/10.2298/GENSR2002689N . .

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Vukadinović, Jelena
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/791
AB  - Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins.
AB  - Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.
PB  - Beograd : Društvo genetičara Srbije
T2  - Genetika
T1  - Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions
T1  - Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena
VL  - 52
IS  - 1
SP  - 273
EP  - 289
DO  - 10.2298/GENSR2001273V
ER  - 

@article{
author = "Vančetović, Jelena and Kostadinović, Marija and Božinović, Sofija and Nikolić, Ana and Vukadinović, Jelena and Marković, Ksenija and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins., Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "Genetika",
title = "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions, Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena",
volume = "52",
number = "1",
pages = "273-289",
doi = "10.2298/GENSR2001273V"
}

Vančetović, J., Kostadinović, M., Božinović, S., Nikolić, A., Vukadinović, J., Marković, K.,& Ignjatović-Micić, D.. (2020). Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika
Beograd : Društvo genetičara Srbije., 52(1), 273-289.
https://doi.org/10.2298/GENSR2001273V

Vančetović J, Kostadinović M, Božinović S, Nikolić A, Vukadinović J, Marković K, Ignjatović-Micić D. Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika. 2020;52(1):273-289.
doi:10.2298/GENSR2001273V .

Vančetović, Jelena, Kostadinović, Marija, Božinović, Sofija, Nikolić, Ana, Vukadinović, Jelena, Marković, Ksenija, Ignjatović-Micić, Dragana, "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions" in Genetika, 52, no. 1 (2020):273-289,
https://doi.org/10.2298/GENSR2001273V . .

TY  - CONF
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Dodig, Dejan
AU  - Kandić, Vesna
AU  - Đorđević-Melnik, Olivera
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/1123
AB  - In this research an influence of the strong source restriction meaning cutting off the whole
plants at the first internodes 5 (5DAPt), 10 (10DAPt) and 15 (15DAPt) days after pollination
on grain yield and its corresponding traits in maize were tested. Control represented plants
harvested at full maturity. Four inbred lines were used, two historical ones (Mo17 and B73)
and two commercial ZP inbreds (ZPL and ZPB). The experiment was conducted at Zemun
Polje, Serbia, in 2014 and 2015. The trait of particular importance was the number of kernels
per ear and its average values were 37.73 at 5DAPt, 115.14 at 10DAPt and 175.20 at 15DAPt,
being sufficient for planting next generation of breeding. According to the results obtained,
ZPL represented an improved Lancaster line over Mo17 regarding drought tolerance, that
could not be stated for ZPB over B73. Hybrid among these two lines is drought tolerant due to
heterosis (epistatic effects) or dominant origin of ZPLs good response to drought stress.
Values for seed set and eventually for grain yield per plant were 0.00 for line B73 at 5DAPt in
2015. An improved breeding scheme for increased drought tolerance could be proposed,
namely self-pollination of border plants on high density sown selfing progenies or dihaploid
(DH) lines, cutting off selfed plants at 15 DAP and evaluating their kernel properties. Openpollinated progeny would serve to estimate other important traits for selection. From the
chosen progenies kernels of selfed and cut-off plants should be used for the next generation of
breeding.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings
T1  - The influence of plant cutting on grain yield traits in maize
SP  - 580
EP  - 585
UR  - https://hdl.handle.net/21.15107/rcub_rik_1123
ER  - 

@conference{
author = "Vančetović, Jelena and Ignjatović-Micić, Dragana and Božinović, Sofija and Nikolić, Ana and Dodig, Dejan and Kandić, Vesna and Đorđević-Melnik, Olivera",
year = "2019",
abstract = "In this research an influence of the strong source restriction meaning cutting off the whole
plants at the first internodes 5 (5DAPt), 10 (10DAPt) and 15 (15DAPt) days after pollination
on grain yield and its corresponding traits in maize were tested. Control represented plants
harvested at full maturity. Four inbred lines were used, two historical ones (Mo17 and B73)
and two commercial ZP inbreds (ZPL and ZPB). The experiment was conducted at Zemun
Polje, Serbia, in 2014 and 2015. The trait of particular importance was the number of kernels
per ear and its average values were 37.73 at 5DAPt, 115.14 at 10DAPt and 175.20 at 15DAPt,
being sufficient for planting next generation of breeding. According to the results obtained,
ZPL represented an improved Lancaster line over Mo17 regarding drought tolerance, that
could not be stated for ZPB over B73. Hybrid among these two lines is drought tolerant due to
heterosis (epistatic effects) or dominant origin of ZPLs good response to drought stress.
Values for seed set and eventually for grain yield per plant were 0.00 for line B73 at 5DAPt in
2015. An improved breeding scheme for increased drought tolerance could be proposed,
namely self-pollination of border plants on high density sown selfing progenies or dihaploid
(DH) lines, cutting off selfed plants at 15 DAP and evaluating their kernel properties. Openpollinated progeny would serve to estimate other important traits for selection. From the
chosen progenies kernels of selfed and cut-off plants should be used for the next generation of
breeding.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings",
title = "The influence of plant cutting on grain yield traits in maize",
pages = "580-585",
url = "https://hdl.handle.net/21.15107/rcub_rik_1123"
}

Vančetović, J., Ignjatović-Micić, D., Božinović, S., Nikolić, A., Dodig, D., Kandić, V.,& Đorđević-Melnik, O.. (2019). The influence of plant cutting on grain yield traits in maize. in 10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 580-585.
https://hdl.handle.net/21.15107/rcub_rik_1123

Vančetović J, Ignjatović-Micić D, Božinović S, Nikolić A, Dodig D, Kandić V, Đorđević-Melnik O. The influence of plant cutting on grain yield traits in maize. in 10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings. 2019;:580-585.
https://hdl.handle.net/21.15107/rcub_rik_1123 .

Vančetović, Jelena, Ignjatović-Micić, Dragana, Božinović, Sofija, Nikolić, Ana, Dodig, Dejan, Kandić, Vesna, Đorđević-Melnik, Olivera, "The influence of plant cutting on grain yield traits in maize" in 10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings (2019):580-585,
https://hdl.handle.net/21.15107/rcub_rik_1123 .

TY  - CONF
AU  - Božić, Manja
AU  - Nikolić, Ana
AU  - Dudić, Dragana
AU  - Samardžić, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Banović Đeri, Bojana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/1145
AB  - As one of the most important metabolic processes in all plants, photosynthesis is necessary
for their survival and ensuring enough energy is created for successful execution of all
physiological processes, including nutrient intake and transport, interactions with the
environment, growth and development, as well as processes important for crops and their
production such as flowering, reproduction, grain filling etc. Many current strategies of
avoiding yield loss in crop production include earlier sowing, as to avoid increasingly higher
temperatures during late spring and summer, the consequences of climate change.
Because of that, the knowledge of the impact of the cold on the process of photosynthesis
is of crucial importance. In this research, we focused on the light dependent phase of
photosynthesis and chose two genes related both to this phase and stress tolerance –
photosystem II cytochrome b559 α-subunit and ATP synthase CF0 A subunit. These
genes were chosen for further research from a set of differentially expressed genes
(DEGs) detected between Lancaster and non-Lancaster maize lines. Their expression was
validated on four maize inbred lines that belonged to different heterotic groups
(Lancaster and non-Lancaster). The experiment was performed under optimal and low
temperatures, 22° and 6° respectively, during the V4 growth stage. Samples for RNA
extraction, cDNA synthesis and qPCR expression validation were taken after 6h and 24h
of exposure to experimental temperatures, as well as after 48h in recovery. Results
showed significant changes in the expression of these genes, both between different time
points of stress treatment, as well as between different inbred lines. These preliminary
results give a good base for further studies of gene expression in important maize lines,
particularly abiotic stress related genes, as a tool for establishing successful breeding
programs and defining pathways involved in maize response to cold stress.
PB  - Belgrade : Serbian Genetic Society
C3  - 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia, October 13-17 2019. Book of Abstracts
T1  - Expression validation of two genes important for photosynthesis under low temperature conditions in different maize inbred lines
UR  - https://hdl.handle.net/21.15107/rcub_rik_1145
ER  - 

@conference{
author = "Božić, Manja and Nikolić, Ana and Dudić, Dragana and Samardžić, Jelena and Ignjatović-Micić, Dragana and Vančetović, Jelena and Banović Đeri, Bojana",
year = "2019",
abstract = "As one of the most important metabolic processes in all plants, photosynthesis is necessary
for their survival and ensuring enough energy is created for successful execution of all
physiological processes, including nutrient intake and transport, interactions with the
environment, growth and development, as well as processes important for crops and their
production such as flowering, reproduction, grain filling etc. Many current strategies of
avoiding yield loss in crop production include earlier sowing, as to avoid increasingly higher
temperatures during late spring and summer, the consequences of climate change.
Because of that, the knowledge of the impact of the cold on the process of photosynthesis
is of crucial importance. In this research, we focused on the light dependent phase of
photosynthesis and chose two genes related both to this phase and stress tolerance –
photosystem II cytochrome b559 α-subunit and ATP synthase CF0 A subunit. These
genes were chosen for further research from a set of differentially expressed genes
(DEGs) detected between Lancaster and non-Lancaster maize lines. Their expression was
validated on four maize inbred lines that belonged to different heterotic groups
(Lancaster and non-Lancaster). The experiment was performed under optimal and low
temperatures, 22° and 6° respectively, during the V4 growth stage. Samples for RNA
extraction, cDNA synthesis and qPCR expression validation were taken after 6h and 24h
of exposure to experimental temperatures, as well as after 48h in recovery. Results
showed significant changes in the expression of these genes, both between different time
points of stress treatment, as well as between different inbred lines. These preliminary
results give a good base for further studies of gene expression in important maize lines,
particularly abiotic stress related genes, as a tool for establishing successful breeding
programs and defining pathways involved in maize response to cold stress.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia, October 13-17 2019. Book of Abstracts",
title = "Expression validation of two genes important for photosynthesis under low temperature conditions in different maize inbred lines",
url = "https://hdl.handle.net/21.15107/rcub_rik_1145"
}

Božić, M., Nikolić, A., Dudić, D., Samardžić, J., Ignjatović-Micić, D., Vančetović, J.,& Banović Đeri, B.. (2019). Expression validation of two genes important for photosynthesis under low temperature conditions in different maize inbred lines. in 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia, October 13-17 2019. Book of Abstracts
Belgrade : Serbian Genetic Society..
https://hdl.handle.net/21.15107/rcub_rik_1145

Božić M, Nikolić A, Dudić D, Samardžić J, Ignjatović-Micić D, Vančetović J, Banović Đeri B. Expression validation of two genes important for photosynthesis under low temperature conditions in different maize inbred lines. in 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia, October 13-17 2019. Book of Abstracts. 2019;.
https://hdl.handle.net/21.15107/rcub_rik_1145 .

Božić, Manja, Nikolić, Ana, Dudić, Dragana, Samardžić, Jelena, Ignjatović-Micić, Dragana, Vančetović, Jelena, Banović Đeri, Bojana, "Expression validation of two genes important for photosynthesis under low temperature conditions in different maize inbred lines" in 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia, October 13-17 2019. Book of Abstracts (2019),
https://hdl.handle.net/21.15107/rcub_rik_1145 .

TY  - CONF
AU  - Banović Đeri, Bojana
AU  - Džodanović, Lana
AU  - Janić, Pavle
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Samardžić, Jelena
AU  - Nikolić, Ana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/1125
AB  - In plants, the expression of mitochondrial and chloroplast genomes is continuously changing
to enable adequate functional adjustments related to overall metabolic changes needed for
plant adaptation to fluctuating environmental conditions. In order to identify mitochondrial
and chloroplast transcripts expressed prior to stresses, we have analyzed mitochondrial and
chloroplast transcriptomes of 46 maize inbred lines chosen from three main heterotic groups
(BSSS, Lancaster, Iowa dent) regularly used in elite maize breeding programs. Organelles’
data were extracted from transcriptome data obtained by next generation sequencing of
total leaf transcriptomes of 46 maize inbred lines grown under the optimal conditions in the
greenhouse. All data manipulation and differential gene expression analysis were performed
by using Galaxy software (https://usegalaxy.eu/). Detected organellar complex transcripts
milieus were expected to contain candidates that modulate responses of their genomes
by activating certain signaling pathways upon exposure to abiotic stresses. With the aim
to identify such candidates we focused on selecting transcripts that were differentially
expressed between 46 maize inbred lines grown under the optimal breeding conditions and
also annotated as important for abiotic stresses response in different plant species. Selected
transcripts/genes were then subjected to freely available tools for gene networking analysis
with the aim to depict possible anterograde and retrograde signaling between nucleus and
mitochondria/chloroplasts under the abiotic stresses. Obtained results are promising in terms
of planning biological experiments for testing the roles of genes that were bioinformatically
predicted as novel members of abiotic stress signaling network between nucleus and
organelles in maize.
PB  - Belgrade : Serbian Genetics Society
C3  - Book of Abstracts, 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia
T1  - Bioinformatics prediction of mitochondrial and chloroplast transcripts possibly involved in maize abiotic stress signaling
SP  - 264
EP  - 264
UR  - https://hdl.handle.net/21.15107/rcub_rik_1125
ER  - 

@conference{
author = "Banović Đeri, Bojana and Džodanović, Lana and Janić, Pavle and Vančetović, Jelena and Ignjatović-Micić, Dragana and Samardžić, Jelena and Nikolić, Ana",
year = "2019",
abstract = "In plants, the expression of mitochondrial and chloroplast genomes is continuously changing
to enable adequate functional adjustments related to overall metabolic changes needed for
plant adaptation to fluctuating environmental conditions. In order to identify mitochondrial
and chloroplast transcripts expressed prior to stresses, we have analyzed mitochondrial and
chloroplast transcriptomes of 46 maize inbred lines chosen from three main heterotic groups
(BSSS, Lancaster, Iowa dent) regularly used in elite maize breeding programs. Organelles’
data were extracted from transcriptome data obtained by next generation sequencing of
total leaf transcriptomes of 46 maize inbred lines grown under the optimal conditions in the
greenhouse. All data manipulation and differential gene expression analysis were performed
by using Galaxy software (https://usegalaxy.eu/). Detected organellar complex transcripts
milieus were expected to contain candidates that modulate responses of their genomes
by activating certain signaling pathways upon exposure to abiotic stresses. With the aim
to identify such candidates we focused on selecting transcripts that were differentially
expressed between 46 maize inbred lines grown under the optimal breeding conditions and
also annotated as important for abiotic stresses response in different plant species. Selected
transcripts/genes were then subjected to freely available tools for gene networking analysis
with the aim to depict possible anterograde and retrograde signaling between nucleus and
mitochondria/chloroplasts under the abiotic stresses. Obtained results are promising in terms
of planning biological experiments for testing the roles of genes that were bioinformatically
predicted as novel members of abiotic stress signaling network between nucleus and
organelles in maize.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Book of Abstracts, 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia",
title = "Bioinformatics prediction of mitochondrial and chloroplast transcripts possibly involved in maize abiotic stress signaling",
pages = "264-264",
url = "https://hdl.handle.net/21.15107/rcub_rik_1125"
}

Banović Đeri, B., Džodanović, L., Janić, P., Vančetović, J., Ignjatović-Micić, D., Samardžić, J.,& Nikolić, A.. (2019). Bioinformatics prediction of mitochondrial and chloroplast transcripts possibly involved in maize abiotic stress signaling. in Book of Abstracts, 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia
Belgrade : Serbian Genetics Society., 264-264.
https://hdl.handle.net/21.15107/rcub_rik_1125

Banović Đeri B, Džodanović L, Janić P, Vančetović J, Ignjatović-Micić D, Samardžić J, Nikolić A. Bioinformatics prediction of mitochondrial and chloroplast transcripts possibly involved in maize abiotic stress signaling. in Book of Abstracts, 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia. 2019;:264-264.
https://hdl.handle.net/21.15107/rcub_rik_1125 .

Banović Đeri, Bojana, Džodanović, Lana, Janić, Pavle, Vančetović, Jelena, Ignjatović-Micić, Dragana, Samardžić, Jelena, Nikolić, Ana, "Bioinformatics prediction of mitochondrial and chloroplast transcripts possibly involved in maize abiotic stress signaling" in Book of Abstracts, 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia (2019):264-264,
https://hdl.handle.net/21.15107/rcub_rik_1125 .

TY  - CONF
AU  - Banović Đeri, Bojana
AU  - Dudić, Dragana
AU  - Božić, Manja
AU  - Janić, Pavle
AU  - Džodanović, Lana
AU  - Samardžić, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Dodig, Dejan
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/1126
AB  - Abiotic stresses cause great losses in crop production worldwide. Due to climatic changes
temperatures higher than the average are frequently observed in temperate areas in the
course of maize flowering and grain filling period, displaying detrimental effect on maize yield.
In order to overcome this problem development of drought tolerant maize lines, but also
cold tolerant maize lines that would allow earlier sowing and hence avoidance of extremely
high temperatures during sensitive developmental phases in maize, became important goal
of maize breeding programs in temperate areas. A set of 46 maize inbred lines was chosen
from three main heterotic groups (BSSS, Lancaster, Iowa dent) important for commercial
breeding programs. Plants were grown under the optimal conditions in the greenhouse and
sampled upon entering V5 growth stage. A total RNA isolated from leaves of three plants of
each inbred line was used for DNA library preparation by Illumina TruSeq Stranded RNA LT
kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent
kit v2 (2x150bp). For data manipulation and analysis a custom made bioinformatics pipeline
was used. Differential gene expression analysis revealed a minimum of 21 genes that were
strongly statistically supported for differential expression between the inbred lines and also
annotated as involved in abiotic stress responses in other plant species. We tested 10 of
these genes in maize by using qPCR on the subset of 8 inbred lines grown under optimal, cold
and drought conditions. Real-time PCR results revealed significant change in the expression
level of tested genes under abiotic stresses, supporting bioinformatics prediction. Verified
genes were then submitted to free online gene networking tools and after determining
relationships between predicted networks and inbred line phenotypes, possible novel gene
candidates for enriching maize abiotic stress signaling network resolution were proposed.
PB  - Belgrade : Serbian Genetics Society
C3  - 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia - Book of Abstracts
T1  - Leaf total transcriptome analysis enriches insight into maize abiotic stress signaling network
SP  - 260
EP  - 260
UR  - https://hdl.handle.net/21.15107/rcub_rik_1126
ER  - 

@conference{
author = "Banović Đeri, Bojana and Dudić, Dragana and Božić, Manja and Janić, Pavle and Džodanović, Lana and Samardžić, Jelena and Ignjatović-Micić, Dragana and Vančetović, Jelena and Božinović, Sofija and Dodig, Dejan and Delić, Nenad and Nikolić, Ana",
year = "2019",
abstract = "Abiotic stresses cause great losses in crop production worldwide. Due to climatic changes
temperatures higher than the average are frequently observed in temperate areas in the
course of maize flowering and grain filling period, displaying detrimental effect on maize yield.
In order to overcome this problem development of drought tolerant maize lines, but also
cold tolerant maize lines that would allow earlier sowing and hence avoidance of extremely
high temperatures during sensitive developmental phases in maize, became important goal
of maize breeding programs in temperate areas. A set of 46 maize inbred lines was chosen
from three main heterotic groups (BSSS, Lancaster, Iowa dent) important for commercial
breeding programs. Plants were grown under the optimal conditions in the greenhouse and
sampled upon entering V5 growth stage. A total RNA isolated from leaves of three plants of
each inbred line was used for DNA library preparation by Illumina TruSeq Stranded RNA LT
kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent
kit v2 (2x150bp). For data manipulation and analysis a custom made bioinformatics pipeline
was used. Differential gene expression analysis revealed a minimum of 21 genes that were
strongly statistically supported for differential expression between the inbred lines and also
annotated as involved in abiotic stress responses in other plant species. We tested 10 of
these genes in maize by using qPCR on the subset of 8 inbred lines grown under optimal, cold
and drought conditions. Real-time PCR results revealed significant change in the expression
level of tested genes under abiotic stresses, supporting bioinformatics prediction. Verified
genes were then submitted to free online gene networking tools and after determining
relationships between predicted networks and inbred line phenotypes, possible novel gene
candidates for enriching maize abiotic stress signaling network resolution were proposed.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia - Book of Abstracts",
title = "Leaf total transcriptome analysis enriches insight into maize abiotic stress signaling network",
pages = "260-260",
url = "https://hdl.handle.net/21.15107/rcub_rik_1126"
}

Banović Đeri, B., Dudić, D., Božić, M., Janić, P., Džodanović, L., Samardžić, J., Ignjatović-Micić, D., Vančetović, J., Božinović, S., Dodig, D., Delić, N.,& Nikolić, A.. (2019). Leaf total transcriptome analysis enriches insight into maize abiotic stress signaling network. in 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia - Book of Abstracts
Belgrade : Serbian Genetics Society., 260-260.
https://hdl.handle.net/21.15107/rcub_rik_1126

Banović Đeri B, Dudić D, Božić M, Janić P, Džodanović L, Samardžić J, Ignjatović-Micić D, Vančetović J, Božinović S, Dodig D, Delić N, Nikolić A. Leaf total transcriptome analysis enriches insight into maize abiotic stress signaling network. in 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia - Book of Abstracts. 2019;:260-260.
https://hdl.handle.net/21.15107/rcub_rik_1126 .

Banović Đeri, Bojana, Dudić, Dragana, Božić, Manja, Janić, Pavle, Džodanović, Lana, Samardžić, Jelena, Ignjatović-Micić, Dragana, Vančetović, Jelena, Božinović, Sofija, Dodig, Dejan, Delić, Nenad, Nikolić, Ana, "Leaf total transcriptome analysis enriches insight into maize abiotic stress signaling network" in 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia - Book of Abstracts (2019):260-260,
https://hdl.handle.net/21.15107/rcub_rik_1126 .

TY  - JOUR
AU  - Dodig, Dejan
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Zorić, Miroslav
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Delić, Nenad
AU  - Weigelt-Fischer, Kathleen
AU  - Junker, Astrid
AU  - Altmann, Thomas
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/812
AB  - Phenotypic measurements under controlled cultivation conditions are essential to gain
a mechanistic understanding of plant responses to environmental impacts and thus
for knowledge-based improvement of their performance under natural field conditions.
Twenty maize inbred lines (ILs) were phenotyped in response to two levels of water and
nitrogen supply (control and stress) and combined nitrogen and water deficit. Over a
course of 5 weeks (from about 4-leaf stage to the beginning of the reproductive stage),
maize phenology and growth were monitored by using a high-throughput phenotyping
platform for daily acquisition of images in different spectral ranges. The focus of the
present study is on the measurements taken at the time of maximum water stress (for
traits that reflect plant physiological properties) and at the end of the experiment (for
traits that reflect plant architectural and biomass-related traits). Twenty-five phenotypic
traits extracted from the digital image data that support biological interpretation of
plant growth were selected for their predictive value for mid-season shoot biomass
accumulation. Measured fresh and dry weights after harvest were used to calculate
various indices (water-use efficiency, physiological nitrogen-use efficiency, specific plant
weight) and to establish correlations with image-derived phenotypic features. Also, score
indices based on dry weight were used to identify contrasting ILs in terms of productivity
and tolerance to stress, and their means for image-derived and manually measured traits
were compared. Color-related traits appear to be indicative of plant performance and
photosystem II operating efficiency might be an importance physiological parameter
of biomass accumulation, particularly under severe stress conditions. Also, genotypes
showing greater leaf area may be better adapted to abiotic stress conditions.
PB  - Lausanne : Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress
VL  - 10
SP  - 814
DO  - 10.3389/fpls.2019.00814
ER  - 

@article{
author = "Dodig, Dejan and Božinović, Sofija and Nikolić, Ana and Zorić, Miroslav and Vančetović, Jelena and Ignjatović-Micić, Dragana and Delić, Nenad and Weigelt-Fischer, Kathleen and Junker, Astrid and Altmann, Thomas",
year = "2019",
abstract = "Phenotypic measurements under controlled cultivation conditions are essential to gain
a mechanistic understanding of plant responses to environmental impacts and thus
for knowledge-based improvement of their performance under natural field conditions.
Twenty maize inbred lines (ILs) were phenotyped in response to two levels of water and
nitrogen supply (control and stress) and combined nitrogen and water deficit. Over a
course of 5 weeks (from about 4-leaf stage to the beginning of the reproductive stage),
maize phenology and growth were monitored by using a high-throughput phenotyping
platform for daily acquisition of images in different spectral ranges. The focus of the
present study is on the measurements taken at the time of maximum water stress (for
traits that reflect plant physiological properties) and at the end of the experiment (for
traits that reflect plant architectural and biomass-related traits). Twenty-five phenotypic
traits extracted from the digital image data that support biological interpretation of
plant growth were selected for their predictive value for mid-season shoot biomass
accumulation. Measured fresh and dry weights after harvest were used to calculate
various indices (water-use efficiency, physiological nitrogen-use efficiency, specific plant
weight) and to establish correlations with image-derived phenotypic features. Also, score
indices based on dry weight were used to identify contrasting ILs in terms of productivity
and tolerance to stress, and their means for image-derived and manually measured traits
were compared. Color-related traits appear to be indicative of plant performance and
photosystem II operating efficiency might be an importance physiological parameter
of biomass accumulation, particularly under severe stress conditions. Also, genotypes
showing greater leaf area may be better adapted to abiotic stress conditions.",
publisher = "Lausanne : Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress",
volume = "10",
pages = "814",
doi = "10.3389/fpls.2019.00814"
}

Dodig, D., Božinović, S., Nikolić, A., Zorić, M., Vančetović, J., Ignjatović-Micić, D., Delić, N., Weigelt-Fischer, K., Junker, A.,& Altmann, T.. (2019). Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress. in Frontiers in Plant Science
Lausanne : Frontiers Media SA., 10, 814.
https://doi.org/10.3389/fpls.2019.00814

Dodig D, Božinović S, Nikolić A, Zorić M, Vančetović J, Ignjatović-Micić D, Delić N, Weigelt-Fischer K, Junker A, Altmann T. Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress. in Frontiers in Plant Science. 2019;10:814.
doi:10.3389/fpls.2019.00814 .

Dodig, Dejan, Božinović, Sofija, Nikolić, Ana, Zorić, Miroslav, Vančetović, Jelena, Ignjatović-Micić, Dragana, Delić, Nenad, Weigelt-Fischer, Kathleen, Junker, Astrid, Altmann, Thomas, "Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress" in Frontiers in Plant Science, 10 (2019):814,
https://doi.org/10.3389/fpls.2019.00814 . .

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/743
AB  - Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije.
AB  - The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Marker assisted backcross breeding in Maize Research Institute Zemun Polje
T1  - Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje
VL  - 25
IS  - 1
SP  - 41
EP  - 47
DO  - 10.5937/SelSem1901041K
ER  - 

@article{
author = "Kostadinović, Marija and Nikolić, Ana and Ristić, Danijela and Božinović, Sofija and Đorđević-Melnik, Olivera and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije., The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Marker assisted backcross breeding in Maize Research Institute Zemun Polje, Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje",
volume = "25",
number = "1",
pages = "41-47",
doi = "10.5937/SelSem1901041K"
}

Kostadinović, M., Nikolić, A., Ristić, D., Božinović, S., Đorđević-Melnik, O., Ignjatović-Micić, D.,& Vančetović, J.. (2019). Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 25(1), 41-47.
https://doi.org/10.5937/SelSem1901041K

Kostadinović M, Nikolić A, Ristić D, Božinović S, Đorđević-Melnik O, Ignjatović-Micić D, Vančetović J. Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo. 2019;25(1):41-47.
doi:10.5937/SelSem1901041K .

Kostadinović, Marija, Nikolić, Ana, Ristić, Danijela, Božinović, Sofija, Đorđević-Melnik, Olivera, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted backcross breeding in Maize Research Institute Zemun Polje" in Selekcija i semenarstvo, 25, no. 1 (2019):41-47,
https://doi.org/10.5937/SelSem1901041K . .

TY  - JOUR
AU  - Kandić, Vesna
AU  - Dodig, Dejan
AU  - Nikolić, Ana
AU  - Kaitović, Željko
AU  - Zorić, Miroslav
AU  - Šurlan-Momirović, Gordana
AU  - Aleksić, Goran
AU  - Đurić, Nenad
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/730
AB  - After the kernel number per spike has been formed during the vegetative stage, grain weight depends on the rate and duration of grain filling. Drought and high temperatures during the grain filling stage frequently occurs in the majority of barley growing regions worldwide. To investigate impact of terminal drought stress on grain filling parameters and grain yield, 15 two- and 10 six-rowed barley genotypes were tested in two-year field trials, set in two locations with two treatments. One treatment was control (C), while in the other treatment (D) terminal drought was simulated by mechanical removal of all leaf blades 7 days after heading of each genotype. In average, defoliation caused reduction of yield (33%), grain filling rate (12%) and 1000-kernel weight (11%). In both treatments, grain yield and the absolute grain filling rate were higher in two-rowed barley genotypes than in six-rowed ones. In both treatments, the correlation was stronger between yield and grain filling duration than between yield and the grain filling rate. The correlation between average yield and grain filling duration was stronger in the D than in the C treatment. According to the results obtained for terminal drought conditions, breeders should create early maturing genotypes of two-rowed type, with a longer grain filling duration and the gradual accumulation of dry matter.
PB  - Patron Editore S R L, Bologna
T2  - Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia
T1  - Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions
VL  - 23
IS  - 2
SP  - 5
EP  - 14
DO  - 10.19199/2018.2.2038-5625.005
ER  - 

@article{
author = "Kandić, Vesna and Dodig, Dejan and Nikolić, Ana and Kaitović, Željko and Zorić, Miroslav and Šurlan-Momirović, Gordana and Aleksić, Goran and Đurić, Nenad",
year = "2018",
abstract = "After the kernel number per spike has been formed during the vegetative stage, grain weight depends on the rate and duration of grain filling. Drought and high temperatures during the grain filling stage frequently occurs in the majority of barley growing regions worldwide. To investigate impact of terminal drought stress on grain filling parameters and grain yield, 15 two- and 10 six-rowed barley genotypes were tested in two-year field trials, set in two locations with two treatments. One treatment was control (C), while in the other treatment (D) terminal drought was simulated by mechanical removal of all leaf blades 7 days after heading of each genotype. In average, defoliation caused reduction of yield (33%), grain filling rate (12%) and 1000-kernel weight (11%). In both treatments, grain yield and the absolute grain filling rate were higher in two-rowed barley genotypes than in six-rowed ones. In both treatments, the correlation was stronger between yield and grain filling duration than between yield and the grain filling rate. The correlation between average yield and grain filling duration was stronger in the D than in the C treatment. According to the results obtained for terminal drought conditions, breeders should create early maturing genotypes of two-rowed type, with a longer grain filling duration and the gradual accumulation of dry matter.",
publisher = "Patron Editore S R L, Bologna",
journal = "Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia",
title = "Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions",
volume = "23",
number = "2",
pages = "5-14",
doi = "10.19199/2018.2.2038-5625.005"
}

Kandić, V., Dodig, D., Nikolić, A., Kaitović, Ž., Zorić, M., Šurlan-Momirović, G., Aleksić, G.,& Đurić, N.. (2018). Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions. in Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia
Patron Editore S R L, Bologna., 23(2), 5-14.
https://doi.org/10.19199/2018.2.2038-5625.005

Kandić V, Dodig D, Nikolić A, Kaitović Ž, Zorić M, Šurlan-Momirović G, Aleksić G, Đurić N. Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions. in Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia. 2018;23(2):5-14.
doi:10.19199/2018.2.2038-5625.005 .

Kandić, Vesna, Dodig, Dejan, Nikolić, Ana, Kaitović, Željko, Zorić, Miroslav, Šurlan-Momirović, Gordana, Aleksić, Goran, Đurić, Nenad, "Grain filling parameters of two- and six-rowed barley genotypes in terminal drought conditions" in Italian Journal of Agrometeorology-Rivista Italiana Di Agrometeorologia, 23, no. 2 (2018):5-14,
https://doi.org/10.19199/2018.2.2038-5625.005 . .

TY  - JOUR
AU  - Dodig, Dejan
AU  - Kandić, Vesna
AU  - Nikolić, Ana
AU  - Zorić, Miroslav
AU  - Nikolic-Dorić, Emilija
AU  - Mutavdzić, Beba
AU  - Perović, Dragan
AU  - Šurlan-Momirović, Gordana
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/707
AB  - Barley (Hordeum vulgare L.) is often grown in sites with low rainfall and high temperature during grain filling. Because spike architecture is one of basic footprints of barley domestication, the importance of spikes in adaptation to different environments or abiotic stresses can be hypothesised. In order to compare different barley spike types in terms of kernel growth and yield components, we tested 15 two-row and 10 six-row winter genotypes in eight environments where terminal drought was simulated by defoliation at 7 days after heading (7 DAH). Control plants were grown intact. On average, two-row genotypes outyielded six-row genotypes by 17% under control conditions and 33% under simulated late drought. Observations of kernel dry weights from 7 DAH through to harvest maturity at 5-day intervals were regressed onto a measure of thermal time. After preliminary evaluation of four nonlinear (S-shaped) models for kernel dry-weight accumulation, the ordinary logistic model was deemed the most appropriate in most cases and was finally applied to all plant-growth curves. Four parameters were estimated from the logistic model. Whereas two earliness estimators (inflection point and thermal time needed to reach maximum kernel weight) were similar for the two barley types, maximum kernel weight (Y-max) and mean rate of kernel growth (RG) were higher (P lt 0.05) in two-row than in six-row barleys. Differences in Y-max and RG among six-row barley genotypes were greater between control and defoliation treatments than between years, whereas among two-row barley genotypes, differences between years were greater, suggesting better stability of six-row types and better drought tolerance of two-row types in the tested barley set.
PB  - Csiro Publishing, Clayton
T2  - Crop & Pasture Science
T1  - Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation
VL  - 69
IS  - 12
SP  - 1215
EP  - 1224
DO  - 10.1071/CP18336
ER  - 

@article{
author = "Dodig, Dejan and Kandić, Vesna and Nikolić, Ana and Zorić, Miroslav and Nikolic-Dorić, Emilija and Mutavdzić, Beba and Perović, Dragan and Šurlan-Momirović, Gordana",
year = "2018",
abstract = "Barley (Hordeum vulgare L.) is often grown in sites with low rainfall and high temperature during grain filling. Because spike architecture is one of basic footprints of barley domestication, the importance of spikes in adaptation to different environments or abiotic stresses can be hypothesised. In order to compare different barley spike types in terms of kernel growth and yield components, we tested 15 two-row and 10 six-row winter genotypes in eight environments where terminal drought was simulated by defoliation at 7 days after heading (7 DAH). Control plants were grown intact. On average, two-row genotypes outyielded six-row genotypes by 17% under control conditions and 33% under simulated late drought. Observations of kernel dry weights from 7 DAH through to harvest maturity at 5-day intervals were regressed onto a measure of thermal time. After preliminary evaluation of four nonlinear (S-shaped) models for kernel dry-weight accumulation, the ordinary logistic model was deemed the most appropriate in most cases and was finally applied to all plant-growth curves. Four parameters were estimated from the logistic model. Whereas two earliness estimators (inflection point and thermal time needed to reach maximum kernel weight) were similar for the two barley types, maximum kernel weight (Y-max) and mean rate of kernel growth (RG) were higher (P lt 0.05) in two-row than in six-row barleys. Differences in Y-max and RG among six-row barley genotypes were greater between control and defoliation treatments than between years, whereas among two-row barley genotypes, differences between years were greater, suggesting better stability of six-row types and better drought tolerance of two-row types in the tested barley set.",
publisher = "Csiro Publishing, Clayton",
journal = "Crop & Pasture Science",
title = "Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation",
volume = "69",
number = "12",
pages = "1215-1224",
doi = "10.1071/CP18336"
}

Dodig, D., Kandić, V., Nikolić, A., Zorić, M., Nikolic-Dorić, E., Mutavdzić, B., Perović, D.,& Šurlan-Momirović, G.. (2018). Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation. in Crop & Pasture Science
Csiro Publishing, Clayton., 69(12), 1215-1224.
https://doi.org/10.1071/CP18336

Dodig D, Kandić V, Nikolić A, Zorić M, Nikolic-Dorić E, Mutavdzić B, Perović D, Šurlan-Momirović G. Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation. in Crop & Pasture Science. 2018;69(12):1215-1224.
doi:10.1071/CP18336 .

Dodig, Dejan, Kandić, Vesna, Nikolić, Ana, Zorić, Miroslav, Nikolic-Dorić, Emilija, Mutavdzić, Beba, Perović, Dragan, Šurlan-Momirović, Gordana, "Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation" in Crop & Pasture Science, 69, no. 12 (2018):1215-1224,
https://doi.org/10.1071/CP18336 . .

TY  - JOUR
AU  - Babić, Vojka
AU  - Kravić, Natalija
AU  - Srdić, Jelena
AU  - Popović, Aleksandar
AU  - Nikolić, Ana
AU  - Zivić, Jovana
AU  - Miritescu, Mihai
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/731
AB  - White maize had an important role in human nutrition throughout the Western Balkan region. A great number of farmers (approximately 86% in Serbia) still grow traditional white Open Pollinated Varieties (OPVs) for human diet. Out of 2217 maize landraces stored within Maize Research Institute "Zemun Polje" (MRIZP) gene bank, more than 700 are white kernel accessions. Eighteen white maize landraces, collected in the Western Balkan region in different periods, were selected for the present study. By the evaluation of agromorphological performances and the basic chemical composition of grain, the objective was to determine the importance of landraces conserved under in situ and ex situ conditions, to be used as the initial material for breeding. The idea was to determine whether the varieties conserved under ex situ conditions (collected from the same or different regions) distinguish from varieties permanently grown in the region of western Serbia. It was found that varieties permanently grown in the western Serbia were more similar to varieties of the ex situ collection from eastern Bosnia and Herzegovina than to varieties collected in western Serbia 50 years ago. The comparison between OPVs and modern white maize hybrids for the basic chemical composition of grain showed the significant potential of OPVs for nutritive grain quality improvement. Increased organic production creates new possibilities for more intensive incorporation of OPVs into this agricultural system. New opportunities for the cooperation and share of responsibilities among breeders, farmers and genetic resources managers are initiated, to be used for in situ maize genetic resources conservation improvement. More detailed characterisation and evaluation of the white maize landraces will offer an explanation of what has motivated our farmers to maintain the OPVs production through centuries, despite the availability of modern hybrids.
PB  - Natl Agricultural Research & Development Inst, Fundulea
T2  - Romanian Agricultural Research
T1  - Grain quality within ex situ and in situ conserved traditional white maize landraces
VL  - 35
SP  - 129
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_rik_731
ER  - 

@article{
author = "Babić, Vojka and Kravić, Natalija and Srdić, Jelena and Popović, Aleksandar and Nikolić, Ana and Zivić, Jovana and Miritescu, Mihai",
year = "2018",
abstract = "White maize had an important role in human nutrition throughout the Western Balkan region. A great number of farmers (approximately 86% in Serbia) still grow traditional white Open Pollinated Varieties (OPVs) for human diet. Out of 2217 maize landraces stored within Maize Research Institute "Zemun Polje" (MRIZP) gene bank, more than 700 are white kernel accessions. Eighteen white maize landraces, collected in the Western Balkan region in different periods, were selected for the present study. By the evaluation of agromorphological performances and the basic chemical composition of grain, the objective was to determine the importance of landraces conserved under in situ and ex situ conditions, to be used as the initial material for breeding. The idea was to determine whether the varieties conserved under ex situ conditions (collected from the same or different regions) distinguish from varieties permanently grown in the region of western Serbia. It was found that varieties permanently grown in the western Serbia were more similar to varieties of the ex situ collection from eastern Bosnia and Herzegovina than to varieties collected in western Serbia 50 years ago. The comparison between OPVs and modern white maize hybrids for the basic chemical composition of grain showed the significant potential of OPVs for nutritive grain quality improvement. Increased organic production creates new possibilities for more intensive incorporation of OPVs into this agricultural system. New opportunities for the cooperation and share of responsibilities among breeders, farmers and genetic resources managers are initiated, to be used for in situ maize genetic resources conservation improvement. More detailed characterisation and evaluation of the white maize landraces will offer an explanation of what has motivated our farmers to maintain the OPVs production through centuries, despite the availability of modern hybrids.",
publisher = "Natl Agricultural Research & Development Inst, Fundulea",
journal = "Romanian Agricultural Research",
title = "Grain quality within ex situ and in situ conserved traditional white maize landraces",
volume = "35",
pages = "129-140",
url = "https://hdl.handle.net/21.15107/rcub_rik_731"
}

Babić, V., Kravić, N., Srdić, J., Popović, A., Nikolić, A., Zivić, J.,& Miritescu, M.. (2018). Grain quality within ex situ and in situ conserved traditional white maize landraces. in Romanian Agricultural Research
Natl Agricultural Research & Development Inst, Fundulea., 35, 129-140.
https://hdl.handle.net/21.15107/rcub_rik_731

Babić V, Kravić N, Srdić J, Popović A, Nikolić A, Zivić J, Miritescu M. Grain quality within ex situ and in situ conserved traditional white maize landraces. in Romanian Agricultural Research. 2018;35:129-140.
https://hdl.handle.net/21.15107/rcub_rik_731 .

Babić, Vojka, Kravić, Natalija, Srdić, Jelena, Popović, Aleksandar, Nikolić, Ana, Zivić, Jovana, Miritescu, Mihai, "Grain quality within ex situ and in situ conserved traditional white maize landraces" in Romanian Agricultural Research, 35 (2018):129-140,
https://hdl.handle.net/21.15107/rcub_rik_731 .

TY  - JOUR
AU  - Anđelković, Violeta
AU  - Nikolić, Ana
AU  - Kovačević, Dragan
AU  - Mladenović Drinić, Snežana
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Srebrić, Mirjana
AU  - Jankulovska, Mirjana
AU  - Ivanovska, Sonja
AU  - Bosev, Dane
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/713
AB  - In the second half of 20th century the awareness of importance of landraces for the future, led to organized collecting missions for numerous plant species. A total of 2217 maize (Zea mays L.) landraces, collected in the former Yugoslavia, are stored at Maize Research Institute (MRIZP) gene bank. During 2014, new collecting missions were organized in the eastern and western parts of Macedonia. According to collecting site and kernel type, 14 samples from the Faculty of Agricultural Sciences and Food, R. Macedonia were chosen for the comparison and identification of possible duplicates, through coupling with the 16 MRIZP gene bank accessions from the same area and kernel characteristics. Phenotypic characterization was done for 21 traits according to International Board for Plant Genetic Resources descriptors for maize. The Principal Component Analysis (PCA) identifies five PCs with Eigenvalue > 1, explaining 80% of the total phenotypic variation. The most discriminative traits with the strongest positive associations were tasseling and silking dates, plant height, leaf length and ear length. Compared to the ex-situ populations, the number of alleles and the number of specific alleles, showed a significant decrease in the in situ populations. Twelve unique alleles were detected in samples from MRIZP gene bank, and only four were found in new Macedonian samples. Cluster analysis of morphological and molecular markers distinguished groups of maize accessions with distinctive morphological traits and genetic profiles that will be useful for conservation, and management of gene bank collection, as well as for possible utilization in breeding.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers
VL  - 78
IS  - 1
SP  - 30
EP  - 38
DO  - 10.4067/S0718-58392018000100030
ER  - 

@article{
author = "Anđelković, Violeta and Nikolić, Ana and Kovačević, Dragan and Mladenović Drinić, Snežana and Kravić, Natalija and Babić, Vojka and Srebrić, Mirjana and Jankulovska, Mirjana and Ivanovska, Sonja and Bosev, Dane",
year = "2018",
abstract = "In the second half of 20th century the awareness of importance of landraces for the future, led to organized collecting missions for numerous plant species. A total of 2217 maize (Zea mays L.) landraces, collected in the former Yugoslavia, are stored at Maize Research Institute (MRIZP) gene bank. During 2014, new collecting missions were organized in the eastern and western parts of Macedonia. According to collecting site and kernel type, 14 samples from the Faculty of Agricultural Sciences and Food, R. Macedonia were chosen for the comparison and identification of possible duplicates, through coupling with the 16 MRIZP gene bank accessions from the same area and kernel characteristics. Phenotypic characterization was done for 21 traits according to International Board for Plant Genetic Resources descriptors for maize. The Principal Component Analysis (PCA) identifies five PCs with Eigenvalue > 1, explaining 80% of the total phenotypic variation. The most discriminative traits with the strongest positive associations were tasseling and silking dates, plant height, leaf length and ear length. Compared to the ex-situ populations, the number of alleles and the number of specific alleles, showed a significant decrease in the in situ populations. Twelve unique alleles were detected in samples from MRIZP gene bank, and only four were found in new Macedonian samples. Cluster analysis of morphological and molecular markers distinguished groups of maize accessions with distinctive morphological traits and genetic profiles that will be useful for conservation, and management of gene bank collection, as well as for possible utilization in breeding.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers",
volume = "78",
number = "1",
pages = "30-38",
doi = "10.4067/S0718-58392018000100030"
}

Anđelković, V., Nikolić, A., Kovačević, D., Mladenović Drinić, S., Kravić, N., Babić, V., Srebrić, M., Jankulovska, M., Ivanovska, S.,& Bosev, D.. (2018). Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 78(1), 30-38.
https://doi.org/10.4067/S0718-58392018000100030

Anđelković V, Nikolić A, Kovačević D, Mladenović Drinić S, Kravić N, Babić V, Srebrić M, Jankulovska M, Ivanovska S, Bosev D. Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers. in Chilean Journal of Agricultural Research. 2018;78(1):30-38.
doi:10.4067/S0718-58392018000100030 .

Anđelković, Violeta, Nikolić, Ana, Kovačević, Dragan, Mladenović Drinić, Snežana, Kravić, Natalija, Babić, Vojka, Srebrić, Mirjana, Jankulovska, Mirjana, Ivanovska, Sonja, Bosev, Dane, "Conserving maize in gene banks: Changes in genetic diversity revealed by morphological and SSR markers" in Chilean Journal of Agricultural Research, 78, no. 1 (2018):30-38,
https://doi.org/10.4067/S0718-58392018000100030 . .

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Kovačević, Dragan
AU  - Mladenović Drinić, Snežana
AU  - Nikolić, Ana
AU  - Mitić, Zorica S.
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/719
AB  - Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites
VL  - 50
IS  - 1
SP  - 69
EP  - 84
DO  - 10.2298/GENSR1801069N
ER  - 

@article{
author = "Nikolić, Biljana and Kovačević, Dragan and Mladenović Drinić, Snežana and Nikolić, Ana and Mitić, Zorica S. and Bojović, Srđan and Marin, Petar D.",
year = "2018",
abstract = "Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites",
volume = "50",
number = "1",
pages = "69-84",
doi = "10.2298/GENSR1801069N"
}

Nikolić, B., Kovačević, D., Mladenović Drinić, S., Nikolić, A., Mitić, Z. S., Bojović, S.,& Marin, P. D.. (2018). Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites. in Genetika
Društvo genetičara Srbije, Beograd., 50(1), 69-84.
https://doi.org/10.2298/GENSR1801069N

Nikolić B, Kovačević D, Mladenović Drinić S, Nikolić A, Mitić ZS, Bojović S, Marin PD. Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites. in Genetika. 2018;50(1):69-84.
doi:10.2298/GENSR1801069N .

Nikolić, Biljana, Kovačević, Dragan, Mladenović Drinić, Snežana, Nikolić, Ana, Mitić, Zorica S., Bojović, Srđan, Marin, Petar D., "Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites" in Genetika, 50, no. 1 (2018):69-84,
https://doi.org/10.2298/GENSR1801069N . .