Stanković, Slavica

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Authority KeyName Variants
orcid::0000-0001-7901-1121
  • Stanković, Slavica (75)
Projects
Reduction of toxigenic Fusarium species and their mycotoxins in production of safe cereal-based foods Implementation of new biotechnological solution in breeding of cattle, sheep and goats for the purpose of obtaining biologically valuable and safe food
Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness Proučavanje i čuvanje core kolekcije fitopatogenih gljiva značajnih u poljoprivredi Srbije
Sustainable conventional and revitalized traditional production of poultry meat and eggs with added value Improvment of maize and soybean traits by molecular and conventional breeding
New products based on cereals and pseudocereals from organic production Improvement of genetic potential and technologies in forage crops production in function of sustainable animal husbandry development
Academy of Finland - 126917 Academy of Finland - 131957
Academy of Finland - 250904 Academy of Finland - 252162
Academy of Finland - 266984 Academy of Finland - 267188
CIMO travel grant Danish Directorate for Food, Fisheries and Agri Business - FFS05-3
Direction Generale de l'Agriculture, Direction de la Recherche - D31-1162 Direction Generale de l'Agriculture, Direction de la Recherche - D31-3159
Direction Generale de l'Agriculture, Direction de la Recherche - D31-7055 Federal Ministry of Education and Research (BMBF) (GABI-KANADA), Bonn - FKZ 0313711A
Felix Thornley Cobbold Trust Finnish Ministry of Agriculture and Forestry
German Academic Exchange Service (DAAD), Bonn - A/06/92183 Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade
Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety
Uticaj citoplazmatičke muške sterilnosti i ksenija na prinos zrna i agronomske osobine kukuruza Stvaranje linija i hibrida kukuruza visokog kvaliteta proteina
Selected biological hazards for safety/quality of food of animal origin and control measures from farm to consumer Implementation of various rearing - selection and biotechnological methods in breeding of pigs

Author's Bibliography

First report of Aspergillus parasiticus on Barley Grain in Serbia

Nikolić, Milica; Savić, Iva; Obradović, Ana; Srdić, Jelena; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(St. Paul : The American Phytopathological Society (APS), 2020)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Obradović, Ana
AU  - Srdić, Jelena
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/796
AB  - Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.
PB  - St. Paul : The American Phytopathological Society (APS)
T2  - Plant Disease
T1  - First report of Aspergillus parasiticus on Barley Grain in Serbia
VL  - 104
IS  - 3
SP  - 987
DO  - 10.1094/PDIS-07-19-1364-PDN
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Obradović, Ana and Srdić, Jelena and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2020",
url = "http://rik.mrizp.rs/handle/123456789/796",
abstract = "Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.",
publisher = "St. Paul : The American Phytopathological Society (APS)",
journal = "Plant Disease",
title = "First report of Aspergillus parasiticus on Barley Grain in Serbia",
volume = "104",
number = "3",
pages = "987",
doi = "10.1094/PDIS-07-19-1364-PDN"
}
Nikolić, M., Savić, I., Obradović, A., Srdić, J., Stanković, G., Stevanović, M.,& Stanković, S. (2020). First report of Aspergillus parasiticus on Barley Grain in Serbia.
Plant DiseaseSt. Paul : The American Phytopathological Society (APS)., 104(3), 987.
https://doi.org/10.1094/PDIS-07-19-1364-PDN
Nikolić M, Savić I, Obradović A, Srdić J, Stanković G, Stevanović M, Stanković S. First report of Aspergillus parasiticus on Barley Grain in Serbia. Plant Disease. 2020;104(3):987
Nikolić Milica, Savić Iva, Obradović Ana, Srdić Jelena, Stanković Goran, Stevanović Milan, Stanković Slavica, "First report of Aspergillus parasiticus on Barley Grain in Serbia" 104, no. 3 (2020):987,
https://doi.org/10.1094/PDIS-07-19-1364-PDN .

Mycobiota and aflatoxin B1 in poultry feeds

Krnjaja, Vesna; Petrović, Tanja; Stanković, Slavica; Lukić, Miloš; Škrbić, Zdenka; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Škrbić, Zdenka
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/742
AB  - In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.
AB  - U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Mycobiota and aflatoxin B1 in poultry feeds
T1  - Mikobiota i aflatoksin B1 u hrani za živinu
VL  - 35
IS  - 1
SP  - 61
EP  - 69
DO  - 10.2298/BAH1901061K
ER  - 
@article{
author = "Krnjaja, Vesna and Petrović, Tanja and Stanković, Slavica and Lukić, Miloš and Škrbić, Zdenka and Mandić, Violeta and Bijelić, Zorica",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/742",
abstract = "In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain., U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Mycobiota and aflatoxin B1 in poultry feeds, Mikobiota i aflatoksin B1 u hrani za živinu",
volume = "35",
number = "1",
pages = "61-69",
doi = "10.2298/BAH1901061K"
}
Krnjaja, V., Petrović, T., Stanković, S., Lukić, M., Škrbić, Z., Mandić, V.,& Bijelić, Z. (2019). Mikobiota i aflatoksin B1 u hrani za živinu.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 35(1), 61-69.
https://doi.org/10.2298/BAH1901061K
Krnjaja V, Petrović T, Stanković S, Lukić M, Škrbić Z, Mandić V, Bijelić Z. Mikobiota i aflatoksin B1 u hrani za živinu. Biotechnology in Animal Husbandry. 2019;35(1):61-69
Krnjaja Vesna, Petrović Tanja, Stanković Slavica, Lukić Miloš, Škrbić Zdenka, Mandić Violeta, Bijelić Zorica, "Mikobiota i aflatoksin B1 u hrani za živinu" 35, no. 1 (2019):61-69,
https://doi.org/10.2298/BAH1901061K .

Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains

Krnjaja, Vesna; Mandić, Violeta; Lukić, Miloš; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Vasić, Tanja
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/735
AB  - Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains
VL  - 116
SP  - 126
EP  - 131
DO  - 10.1016/j.cropro.2018.10.021
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lukić, Miloš and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Vasić, Tanja",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/735",
abstract = "Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains",
volume = "116",
pages = "126-131",
doi = "10.1016/j.cropro.2018.10.021"
}
Krnjaja, V., Mandić, V., Lukić, M., Bijelić, Z., Stanković, S., Obradović, A.,& Vasić, T. (2019). Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains.
Crop ProtectionElsevier Sci Ltd, Oxford., 116, 126-131.
https://doi.org/10.1016/j.cropro.2018.10.021
Krnjaja V, Mandić V, Lukić M, Bijelić Z, Stanković S, Obradović A, Vasić T. Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains. Crop Protection. 2019;116:126-131
Krnjaja Vesna, Mandić Violeta, Lukić Miloš, Bijelić Zorica, Stanković Slavica, Obradović Ana, Vasić Tanja, "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains" 116 (2019):126-131,
https://doi.org/10.1016/j.cropro.2018.10.021 .
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Impact of climatic conditions on fumonisins in maize grown in Serbia

Udovicki, B.; Đekić, I.; Rajković, A.; Stanković, Slavica; Obradović, Ana

(Wageningen Academic Publishers, Wageningen, 2019)

TY  - JOUR
AU  - Udovicki, B.
AU  - Đekić, I.
AU  - Rajković, A.
AU  - Stanković, Slavica
AU  - Obradović, Ana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/737
AB  - The influence of climatic conditions on the levels of fumonisins in maize grown in Serbia was studied over eight years (2008 to 2015), investigating the possible relationship between the levels of fumonisins contamination in maize in relation to registered weather/climatic conditions. Presence of these mycotoxins in maize after harvest was evaluated based on climatic conditions within two periods: 10 days before and 10 days after 50% silking. Categories of fumonisins levels were transformed into classes. Chi-square test for association was used in analysing relationships between results of fumonisins levels and calendar years. Mann-Whitney U-test was used to compare differences between meteorological data of two subsets in years with high and low fumonisins level. There was a statistically significant association between the fumonisins levels and calendar years chi(2)=247.954; (P lt 0.05). This study identified low precipitation and low humidity combined with high solar radiation as a critical parameter combination for elevated levels of fumonisins. The statistically significant difference in relation to some of the examined parameters suggests that maize in Serbian agro-climatic conditions is more susceptible to fungal colonisation, and subsequent fumonisin production in the period of 10 days before 50% silking.
PB  - Wageningen Academic Publishers, Wageningen
T2  - World Mycotoxin Journal
T1  - Impact of climatic conditions on fumonisins in maize grown in Serbia
VL  - 12
IS  - 2
SP  - 183
EP  - 190
DO  - 10.3920/WMJ2018.2364
ER  - 
@article{
author = "Udovicki, B. and Đekić, I. and Rajković, A. and Stanković, Slavica and Obradović, Ana",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/737",
abstract = "The influence of climatic conditions on the levels of fumonisins in maize grown in Serbia was studied over eight years (2008 to 2015), investigating the possible relationship between the levels of fumonisins contamination in maize in relation to registered weather/climatic conditions. Presence of these mycotoxins in maize after harvest was evaluated based on climatic conditions within two periods: 10 days before and 10 days after 50% silking. Categories of fumonisins levels were transformed into classes. Chi-square test for association was used in analysing relationships between results of fumonisins levels and calendar years. Mann-Whitney U-test was used to compare differences between meteorological data of two subsets in years with high and low fumonisins level. There was a statistically significant association between the fumonisins levels and calendar years chi(2)=247.954; (P lt 0.05). This study identified low precipitation and low humidity combined with high solar radiation as a critical parameter combination for elevated levels of fumonisins. The statistically significant difference in relation to some of the examined parameters suggests that maize in Serbian agro-climatic conditions is more susceptible to fungal colonisation, and subsequent fumonisin production in the period of 10 days before 50% silking.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "World Mycotoxin Journal",
title = "Impact of climatic conditions on fumonisins in maize grown in Serbia",
volume = "12",
number = "2",
pages = "183-190",
doi = "10.3920/WMJ2018.2364"
}
Udovicki, B., Đekić, I., Rajković, A., Stanković, S.,& Obradović, A. (2019). Impact of climatic conditions on fumonisins in maize grown in Serbia.
World Mycotoxin JournalWageningen Academic Publishers, Wageningen., 12(2), 183-190.
https://doi.org/10.3920/WMJ2018.2364
Udovicki B, Đekić I, Rajković A, Stanković S, Obradović A. Impact of climatic conditions on fumonisins in maize grown in Serbia. World Mycotoxin Journal. 2019;12(2):183-190
Udovicki B., Đekić I., Rajković A., Stanković Slavica, Obradović Ana, "Impact of climatic conditions on fumonisins in maize grown in Serbia" 12, no. 2 (2019):183-190,
https://doi.org/10.3920/WMJ2018.2364 .
5
2
2

Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia

Krnjaja, Vesna; Stanković, Slavica; Lukić, Miloš; Mićić, Nenad; Petrović, Tanja; Bijelić, Zorica; Mandić, Violeta

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Mićić, Nenad
AU  - Petrović, Tanja
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/720
AB  - This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.
AB  - Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia
T1  - Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji
VL  - 34
IS  - 2
SP  - 239
EP  - 249
DO  - 10.2298/bah1802239K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Lukić, Miloš and Mićić, Nenad and Petrović, Tanja and Bijelić, Zorica and Mandić, Violeta",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/720",
abstract = "This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain., Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia, Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji",
volume = "34",
number = "2",
pages = "239-249",
doi = "10.2298/bah1802239K"
}
Krnjaja, V., Stanković, S., Lukić, M., Mićić, N., Petrović, T., Bijelić, Z.,& Mandić, V. (2018). Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 34(2), 239-249.
https://doi.org/10.2298/bah1802239K
Krnjaja V, Stanković S, Lukić M, Mićić N, Petrović T, Bijelić Z, Mandić V. Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji. Biotechnology in Animal Husbandry. 2018;34(2):239-249
Krnjaja Vesna, Stanković Slavica, Lukić Miloš, Mićić Nenad, Petrović Tanja, Bijelić Zorica, Mandić Violeta, "Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji" 34, no. 2 (2018):239-249,
https://doi.org/10.2298/bah1802239K .
1

In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia

Bočarov-Stančić, Aleksandra; Lopičić, Zorica R.; Bodroža-Solarov, Marija I.; Stanković, Slavica; Janković, Snežana; Milojković, Jelena V.; Krulj, Jelena A.

(Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad, 2018)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lopičić, Zorica R.
AU  - Bodroža-Solarov, Marija I.
AU  - Stanković, Slavica
AU  - Janković, Snežana
AU  - Milojković, Jelena V.
AU  - Krulj, Jelena A.
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/718
AB  - Aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON) and T-2 toxin are the most extensively studied toxic fungal metabolites. Once mycotoxins enter the food/feed production chain keeping their toxic characteristics, it is very difficult to remove or eliminate them. One of promising methods to reduce mycotoxins in contaminated food/feedstuffs is the use of mycotoxin binders. This paper presents the results of in vitro investigations of mineral mycotoxin binders (bentonite - BEN, diatomite - DIA and zeolite - ZEO), and organic mycotoxin binders - agricultural waste materials (Myriophillium spicatum, peach and sour cherry pits). Chemical compositions of the adsorbents have showed that they do not consist of elements toxic to the animals. Inorganic adsorbents (BEN, DIA and ZEO) tested in vitro were better binders of AFB1 (94.97% - 96.90%), while the biosorbents were more efficient in adsorption of OTA (19.98% - 66.66%), ZON (33.33% - 75.00%) and T-2 toxin (16.67% - 50.00%). Inorganic adsorbents and organic waste materials expressed similar binding capacity for DON in vitro, with the exception of M. spicatum that did not at all adsorb this type B trichothecene. Our results indicate that feed contamination with different types of mycotoxins might be diminished by a product that combines different inorganic and organic adsorbents with diverse mycotoxin binding properties.
AB  - Aflatoksin B1 (AFB1), ohratoksin A (OTA), zearalenon (ZON), dezoksinivalenol (DON) i T-2 toksin su najviše izučavani toksični metaboliti gljiva. Kada mikotoksini uđu u proizvodni lanac za hranu/hranu za životinje, zadržavajući svoje toksične karakteristike, teško ih je ukloniti ili eliminisati. Jedna od obećavajućih metoda za smanjenje nivoa mikotoksina u kontaminiranoj hrani/hrani za životinje je korišćenje mikotoksinskih veziva. Ovaj rad predstavlja rezultate in vitro istraživanja mineralnih mikotoksinskih veziva (bentonit - BEN, diatomit - DIA i zeolit - ZEO) i organskih veziva mikotoksina - poljoprivrednog otpadnog materijala (Myriophillium spicatum, koštice breskve i višnje). Hemijski sastavi adsorbenata pokazali su da ne sadrže elemente toksične za životinje. Neorganski adsorbenti (BEN, DIA i ZEO) testirani in vitro bolje su vezivali AFB1 (94,97% - 96,90%), dok su biosorbenti bili efikasniji u adsorpciji OTA (19,98% - 66,66%), ZON-a (33,33% - 75,00% ) i T-2 toksina (16,67% - 50,00%). Neorganski adsorbenti i organski otpadni materijali su pokazali sličan kapacitet in vitro vezivanja DON-a, sa izuzetkom M. spicatum koji uopšte nije adsorbovao ovaj trihotecen tipa B. Naši rezultati koji su prikazani ovde pokazuju da zagađivanje hrane i hrane za životinje različitim vrstama mikotoksina može biti smanjeno dodavanjem preparata dobijenog kombinacijom različitih neorganskih i organskih adsorbenata koji poseduju različite karakteristike vezivanja mikotoksina.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia
T1  - In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije
VL  - 45
IS  - 7
SP  - 87
EP  - 96
DO  - 10.5937/FFR1802087B
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lopičić, Zorica R. and Bodroža-Solarov, Marija I. and Stanković, Slavica and Janković, Snežana and Milojković, Jelena V. and Krulj, Jelena A.",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/718",
abstract = "Aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON) and T-2 toxin are the most extensively studied toxic fungal metabolites. Once mycotoxins enter the food/feed production chain keeping their toxic characteristics, it is very difficult to remove or eliminate them. One of promising methods to reduce mycotoxins in contaminated food/feedstuffs is the use of mycotoxin binders. This paper presents the results of in vitro investigations of mineral mycotoxin binders (bentonite - BEN, diatomite - DIA and zeolite - ZEO), and organic mycotoxin binders - agricultural waste materials (Myriophillium spicatum, peach and sour cherry pits). Chemical compositions of the adsorbents have showed that they do not consist of elements toxic to the animals. Inorganic adsorbents (BEN, DIA and ZEO) tested in vitro were better binders of AFB1 (94.97% - 96.90%), while the biosorbents were more efficient in adsorption of OTA (19.98% - 66.66%), ZON (33.33% - 75.00%) and T-2 toxin (16.67% - 50.00%). Inorganic adsorbents and organic waste materials expressed similar binding capacity for DON in vitro, with the exception of M. spicatum that did not at all adsorb this type B trichothecene. Our results indicate that feed contamination with different types of mycotoxins might be diminished by a product that combines different inorganic and organic adsorbents with diverse mycotoxin binding properties., Aflatoksin B1 (AFB1), ohratoksin A (OTA), zearalenon (ZON), dezoksinivalenol (DON) i T-2 toksin su najviše izučavani toksični metaboliti gljiva. Kada mikotoksini uđu u proizvodni lanac za hranu/hranu za životinje, zadržavajući svoje toksične karakteristike, teško ih je ukloniti ili eliminisati. Jedna od obećavajućih metoda za smanjenje nivoa mikotoksina u kontaminiranoj hrani/hrani za životinje je korišćenje mikotoksinskih veziva. Ovaj rad predstavlja rezultate in vitro istraživanja mineralnih mikotoksinskih veziva (bentonit - BEN, diatomit - DIA i zeolit - ZEO) i organskih veziva mikotoksina - poljoprivrednog otpadnog materijala (Myriophillium spicatum, koštice breskve i višnje). Hemijski sastavi adsorbenata pokazali su da ne sadrže elemente toksične za životinje. Neorganski adsorbenti (BEN, DIA i ZEO) testirani in vitro bolje su vezivali AFB1 (94,97% - 96,90%), dok su biosorbenti bili efikasniji u adsorpciji OTA (19,98% - 66,66%), ZON-a (33,33% - 75,00% ) i T-2 toksina (16,67% - 50,00%). Neorganski adsorbenti i organski otpadni materijali su pokazali sličan kapacitet in vitro vezivanja DON-a, sa izuzetkom M. spicatum koji uopšte nije adsorbovao ovaj trihotecen tipa B. Naši rezultati koji su prikazani ovde pokazuju da zagađivanje hrane i hrane za životinje različitim vrstama mikotoksina može biti smanjeno dodavanjem preparata dobijenog kombinacijom različitih neorganskih i organskih adsorbenata koji poseduju različite karakteristike vezivanja mikotoksina.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia, In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije",
volume = "45",
number = "7",
pages = "87-96",
doi = "10.5937/FFR1802087B"
}
Bočarov-Stančić, A., Lopičić, Z. R., Bodroža-Solarov, M. I., Stanković, S., Janković, S., Milojković, J. V.,& Krulj, J. A. (2018). In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije.
Food and Feed ResearchUniverzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 45(7), 87-96.
https://doi.org/10.5937/FFR1802087B
Bočarov-Stančić A, Lopičić ZR, Bodroža-Solarov MI, Stanković S, Janković S, Milojković JV, Krulj JA. In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije. Food and Feed Research. 2018;45(7):87-96
Bočarov-Stančić Aleksandra, Lopičić Zorica R., Bodroža-Solarov Marija I., Stanković Slavica, Janković Snežana, Milojković Jelena V., Krulj Jelena A., "In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije" 45, no. 7 (2018):87-96,
https://doi.org/10.5937/FFR1802087B .

Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat

Nikolić, Milica; Nikolić, Ana; Savić, Iva; Petrović, Tanja; Stanković, Slavica; Jauković, Marko; Bagi, Ferenc

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Nikolić, Ana
AU  - Savić, Iva
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Jauković, Marko
AU  - Bagi, Ferenc
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/709
AB  - The species of the genus Aspergillus, A. flavus and A. parasiticus, are the most aflatoxin-producing fungi. All previous studies carried out under the production conditions of Serbia showed no presence of A. parasiticus on wheat kernel. On the basis of changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, which favour increased frequency of Aspergillus spp., we assumed that this pathogen can also be present in Serbia. The significance of direct losses as a consequence of wheat kernel infection, as well as potential contamination with aflatoxins, have pointed out to the need to determine the presence of toxigenic potential of A. flavus and A. parasiticus isolates originating from Serbia. For that purpose, wheat kernel samples were collected in nine locations. According to morphological, toxicological and molecular traits of isolated fungi, the presence of A. flavus and A. parasiticus was confirmed. This is the first time that A. parasiticus was identified on wheat under climatic conditions in Serbia. This study indicates that these pathogens may be a potential danger in wheat production in the region of Serbia. This danger will be much more certain if global climatic changes continue as they will provide more intensive development of these pathogens.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat
VL  - 50
IS  - 1
SP  - 143
EP  - 152
DO  - 10.2298/GENSR1801143N
ER  - 
@article{
author = "Nikolić, Milica and Nikolić, Ana and Savić, Iva and Petrović, Tanja and Stanković, Slavica and Jauković, Marko and Bagi, Ferenc",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/709",
abstract = "The species of the genus Aspergillus, A. flavus and A. parasiticus, are the most aflatoxin-producing fungi. All previous studies carried out under the production conditions of Serbia showed no presence of A. parasiticus on wheat kernel. On the basis of changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, which favour increased frequency of Aspergillus spp., we assumed that this pathogen can also be present in Serbia. The significance of direct losses as a consequence of wheat kernel infection, as well as potential contamination with aflatoxins, have pointed out to the need to determine the presence of toxigenic potential of A. flavus and A. parasiticus isolates originating from Serbia. For that purpose, wheat kernel samples were collected in nine locations. According to morphological, toxicological and molecular traits of isolated fungi, the presence of A. flavus and A. parasiticus was confirmed. This is the first time that A. parasiticus was identified on wheat under climatic conditions in Serbia. This study indicates that these pathogens may be a potential danger in wheat production in the region of Serbia. This danger will be much more certain if global climatic changes continue as they will provide more intensive development of these pathogens.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat",
volume = "50",
number = "1",
pages = "143-152",
doi = "10.2298/GENSR1801143N"
}
Nikolić, M., Nikolić, A., Savić, I., Petrović, T., Stanković, S., Jauković, M.,& Bagi, F. (2018). Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat.
GenetikaDruštvo genetičara Srbije, Beograd., 50(1), 143-152.
https://doi.org/10.2298/GENSR1801143N
Nikolić M, Nikolić A, Savić I, Petrović T, Stanković S, Jauković M, Bagi F. Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat. Genetika. 2018;50(1):143-152
Nikolić Milica, Nikolić Ana, Savić Iva, Petrović Tanja, Stanković Slavica, Jauković Marko, Bagi Ferenc, "Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat" 50, no. 1 (2018):143-152,
https://doi.org/10.2298/GENSR1801143N .
2
3
2

Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence

Obradović, Ana; Krnjaja, Vesna; Nikolić, Milica; Delibašić, Goran; Filipović, Milomir; Stanković, Goran; Stanković, Slavica

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Nikolić, Milica
AU  - Delibašić, Goran
AU  - Filipović, Milomir
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/738
AB  - Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species.
AB  - Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence
T1  - Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava
VL  - 34
IS  - 4
SP  - 469
EP  - 480
DO  - 10.2298/BAH1804469O
ER  - 
@article{
author = "Obradović, Ana and Krnjaja, Vesna and Nikolić, Milica and Delibašić, Goran and Filipović, Milomir and Stanković, Goran and Stanković, Slavica",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/738",
abstract = "Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species., Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence, Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava",
volume = "34",
number = "4",
pages = "469-480",
doi = "10.2298/BAH1804469O"
}
Obradović, A., Krnjaja, V., Nikolić, M., Delibašić, G., Filipović, M., Stanković, G.,& Stanković, S. (2018). Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 34(4), 469-480.
https://doi.org/10.2298/BAH1804469O
Obradović A, Krnjaja V, Nikolić M, Delibašić G, Filipović M, Stanković G, Stanković S. Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava. Biotechnology in Animal Husbandry. 2018;34(4):469-480
Obradović Ana, Krnjaja Vesna, Nikolić Milica, Delibašić Goran, Filipović Milomir, Stanković Goran, Stanković Slavica, "Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava" 34, no. 4 (2018):469-480,
https://doi.org/10.2298/BAH1804469O .
3

Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia

Krnjaja, Vesna; Mandić, Violeta; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Bozić, Manja

(Mdpi, Basel, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Bozić, Manja
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/702
AB  - Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.
PB  - Mdpi, Basel
T2  - Toxins
T1  - Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia
VL  - 10
IS  - 11
DO  - 10.3390/toxins10110460
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Bozić, Manja",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/702",
abstract = "Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.",
publisher = "Mdpi, Basel",
journal = "Toxins",
title = "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia",
volume = "10",
number = "11",
doi = "10.3390/toxins10110460"
}
Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T.,& Bozić, M. (2018). Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia.
ToxinsMdpi, Basel., 10(11).
https://doi.org/10.3390/toxins10110460
Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Bozić M. Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. Toxins. 2018;10(11)
Krnjaja Vesna, Mandić Violeta, Bijelić Zorica, Stanković Slavica, Obradović Ana, Petrović Tanja, Bozić Manja, "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia" 10, no. 11 (2018),
https://doi.org/10.3390/toxins10110460 .
3
1
1

Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1

Bartolić, Dragana; Stanković, Mira; Mutavdzić, Dragosav; Radotić, Ksenija; Stanković, Slavica; Jovanović, Dragoljub

(Springer/Plenum Publishers, New York, 2018)

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Mutavdzić, Dragosav
AU  - Radotić, Ksenija
AU  - Stanković, Slavica
AU  - Jovanović, Dragoljub
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/722
AB  - In this preliminary study, we used the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) algorithm to analyze the excitation-emission matrix for different samples of maize flour contaminated with aflatoxin B1 (AFB1) - uncontaminated, low-contaminated, high-contaminated and flour from the local market. We intended to see if there are differences in emission spectral parameters that depend on degree of contamination. The analysis used genuine emission of the fluorophores in the flour, in absence and presence of AFB1, which enables fast screening of the samples, without sample pre-processing. As a result of the analysis, two fluorescence components were derived from the emission spectra for all analyzed samples. The components' positions were the same for the uncontaminated reference sample and the commercial flour sample from the local market, whereas for the samples contaminated with the aflatoxin B1, the emitted peaks' positions were red-shifted. We found that the ratio of the areas of these two components is proportional to the intensity of contamination: 0.071 for uncontaminated sample, 0.090 for the sample from local market, 0.192 for low-contaminated sample and 1.431 for high-contaminated sample. These results indicate that fluorescence EEM coupled with MCR-ALS could be used for rapid and simple estimation of the degree AFB1 contamination in maize flour.
PB  - Springer/Plenum Publishers, New York
T2  - Journal of Fluorescence
T1  - Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1
VL  - 28
IS  - 3
SP  - 729
EP  - 733
DO  - 10.1007/s10895-018-2246-z
ER  - 
@article{
author = "Bartolić, Dragana and Stanković, Mira and Mutavdzić, Dragosav and Radotić, Ksenija and Stanković, Slavica and Jovanović, Dragoljub",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/722",
abstract = "In this preliminary study, we used the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) algorithm to analyze the excitation-emission matrix for different samples of maize flour contaminated with aflatoxin B1 (AFB1) - uncontaminated, low-contaminated, high-contaminated and flour from the local market. We intended to see if there are differences in emission spectral parameters that depend on degree of contamination. The analysis used genuine emission of the fluorophores in the flour, in absence and presence of AFB1, which enables fast screening of the samples, without sample pre-processing. As a result of the analysis, two fluorescence components were derived from the emission spectra for all analyzed samples. The components' positions were the same for the uncontaminated reference sample and the commercial flour sample from the local market, whereas for the samples contaminated with the aflatoxin B1, the emitted peaks' positions were red-shifted. We found that the ratio of the areas of these two components is proportional to the intensity of contamination: 0.071 for uncontaminated sample, 0.090 for the sample from local market, 0.192 for low-contaminated sample and 1.431 for high-contaminated sample. These results indicate that fluorescence EEM coupled with MCR-ALS could be used for rapid and simple estimation of the degree AFB1 contamination in maize flour.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Journal of Fluorescence",
title = "Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1",
volume = "28",
number = "3",
pages = "729-733",
doi = "10.1007/s10895-018-2246-z"
}
Bartolić, D., Stanković, M., Mutavdzić, D., Radotić, K., Stanković, S.,& Jovanović, D. (2018). Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1.
Journal of FluorescenceSpringer/Plenum Publishers, New York., 28(3), 729-733.
https://doi.org/10.1007/s10895-018-2246-z
Bartolić D, Stanković M, Mutavdzić D, Radotić K, Stanković S, Jovanović D. Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1. Journal of Fluorescence. 2018;28(3):729-733
Bartolić Dragana, Stanković Mira, Mutavdzić Dragosav, Radotić Ksenija, Stanković Slavica, Jovanović Dragoljub, "Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1" 28, no. 3 (2018):729-733,
https://doi.org/10.1007/s10895-018-2246-z .
2
2
1

Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia

Obradović, Ana; Stanković, Slavica; Nikolić, Ana; Ignjatović-Micić, Dragana; Krnjaja, Vesna; Stepanović, Jelena; Duduk, Bojan

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Krnjaja, Vesna
AU  - Stepanović, Jelena
AU  - Duduk, Bojan
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/682
AB  - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia
VL  - 49
IS  - 1
SP  - 355
EP  - 364
DO  - 10.2298/GENSR1701355O
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Nikolić, Ana and Ignjatović-Micić, Dragana and Krnjaja, Vesna and Stepanović, Jelena and Duduk, Bojan",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/682",
abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia",
volume = "49",
number = "1",
pages = "355-364",
doi = "10.2298/GENSR1701355O"
}
Obradović, A., Stanković, S., Nikolić, A., Ignjatović-Micić, D., Krnjaja, V., Stepanović, J.,& Duduk, B. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia.
GenetikaDruštvo genetičara Srbije, Beograd., 49(1), 355-364.
https://doi.org/10.2298/GENSR1701355O
Obradović A, Stanković S, Nikolić A, Ignjatović-Micić D, Krnjaja V, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. Genetika. 2017;49(1):355-364
Obradović Ana, Stanković Slavica, Nikolić Ana, Ignjatović-Micić Dragana, Krnjaja Vesna, Stepanović Jelena, Duduk Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" 49, no. 1 (2017):355-364,
https://doi.org/10.2298/GENSR1701355O .
3
3
3

Presence of deoxynivalenol in wheat milling products in Serbia during 2016-2017

Jauković, Marko; Zečević, Veselinka; Stanković, Slavica; Krnjaja, Vesna

(Matica srpska, Novi Sad, 2017)

TY  - JOUR
AU  - Jauković, Marko
AU  - Zečević, Veselinka
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/683
AB  - Deoxynivalenol (DON) is one of several mycotoxins produced by certain Fusarium species that frequently infect wheat, corn, rice, oats, barley and other grains in the field or during storage. DON affects animal and human health causing vomiting, acute temporary nausea, diarrhea, abdominal pain, headache, dizziness and fever. The objective of this study was to evaluate the natural occurrence of deoxynivalenol (DON) in white wheat flour, whole wheat flour and wheat bran. In this study, a total of 75 white wheat flour, whole wheat flour and wheat bran samples were collected in the period of 2016-2017. All samples were analyzed for DON by enzyme-linked immunosorbent assay. DON was detected in 23 out of 45 white wheat flour samples (51.11%), at levels ranging from 99 µg/kg to 440 µg/kg. Out of 15 whole wheat flour samples, 14 were contaminated by DON (93.33%), at levels ranging from 98 µg/kg to 479 µg/kg. The maximum contamination level of DON (2,790 µg/kg) in this study was found in wheat bran. Presence of DON was detected in all 15 samples of wheat bran (100%). These results suggest a high percentage of contaminated samples, especially among wheat bran samples, which raises a risk for consumers of wheat bran and the need to monitor final products before consumption.
AB  - Deoksinivalenol (DON) jedan je od nekoliko mikotoksina koje produkuju određene vrste roda Fusarium, koje često kontaminiraju pšenicu, kukuruz, pirinač, ovas, ječam i ostale žitarice, kako u polju, tako i tokom perioda skladištenja. DON utiče na zdravlje ljudi i životinja izazivajući povraćanje, akutne mučnine, dijareju, abdominalni bol, glavobolje, vrtoglavice i groznicu. Cilj ovog istraživanja bio je da se ispita i proceni prirodna pojava deoksini­valenola (DON) u belom pšeničnom brašnu, integralnom pšeničnom brašnu i pšeničnim mekinjama. Ukupno 75 uzoraka belog pšeničnog brašna, integralnog pšeničnog brašna i pšeničnih mekinja uzorkovano je u periodu 2016-2017. Svi uzorci su analizirani na prisustvo DON-a imunoenzimskim ELISA testovima. Prisustvo DON-a je detektovano u 23 od 45 uzoraka belog pšeničnog brašna (51,11%), u koncentracionom opsegu od 99 do 440 µg/kg. Od 15 ispitanih uzoraka integralnog pšeničnog brašna 14 je bilo kontaminirano (93,33%), a koncentracija DON-a kretala se u opsegu od 98 do 479 µg/kg. Maksimalna koncentracija DON-a (2.790 μg/kg) u ovom istraživanju zabeležena je kod pšeničnih mekinja. Prisustvo DON-a detektovano je u svih 15 ispitivanih uzorka pšeničnih mekinja (100%). Ovi rezultati ukazuju na visok procenat kontaminiranih uzoraka, pogotovo kod uzoraka pšeničnih mekinja, što predstavlja rizik po potrošače i iziskuje potrebu za monitoringom finalnih proizvoda pre puštanja u promet.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Presence of deoxynivalenol in wheat milling products in Serbia during 2016-2017
T1  - Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji
IS  - 133
SP  - 57
EP  - 62
DO  - 10.2298/ZMSPN1733057J
ER  - 
@article{
author = "Jauković, Marko and Zečević, Veselinka and Stanković, Slavica and Krnjaja, Vesna",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/683",
abstract = "Deoxynivalenol (DON) is one of several mycotoxins produced by certain Fusarium species that frequently infect wheat, corn, rice, oats, barley and other grains in the field or during storage. DON affects animal and human health causing vomiting, acute temporary nausea, diarrhea, abdominal pain, headache, dizziness and fever. The objective of this study was to evaluate the natural occurrence of deoxynivalenol (DON) in white wheat flour, whole wheat flour and wheat bran. In this study, a total of 75 white wheat flour, whole wheat flour and wheat bran samples were collected in the period of 2016-2017. All samples were analyzed for DON by enzyme-linked immunosorbent assay. DON was detected in 23 out of 45 white wheat flour samples (51.11%), at levels ranging from 99 µg/kg to 440 µg/kg. Out of 15 whole wheat flour samples, 14 were contaminated by DON (93.33%), at levels ranging from 98 µg/kg to 479 µg/kg. The maximum contamination level of DON (2,790 µg/kg) in this study was found in wheat bran. Presence of DON was detected in all 15 samples of wheat bran (100%). These results suggest a high percentage of contaminated samples, especially among wheat bran samples, which raises a risk for consumers of wheat bran and the need to monitor final products before consumption., Deoksinivalenol (DON) jedan je od nekoliko mikotoksina koje produkuju određene vrste roda Fusarium, koje često kontaminiraju pšenicu, kukuruz, pirinač, ovas, ječam i ostale žitarice, kako u polju, tako i tokom perioda skladištenja. DON utiče na zdravlje ljudi i životinja izazivajući povraćanje, akutne mučnine, dijareju, abdominalni bol, glavobolje, vrtoglavice i groznicu. Cilj ovog istraživanja bio je da se ispita i proceni prirodna pojava deoksini­valenola (DON) u belom pšeničnom brašnu, integralnom pšeničnom brašnu i pšeničnim mekinjama. Ukupno 75 uzoraka belog pšeničnog brašna, integralnog pšeničnog brašna i pšeničnih mekinja uzorkovano je u periodu 2016-2017. Svi uzorci su analizirani na prisustvo DON-a imunoenzimskim ELISA testovima. Prisustvo DON-a je detektovano u 23 od 45 uzoraka belog pšeničnog brašna (51,11%), u koncentracionom opsegu od 99 do 440 µg/kg. Od 15 ispitanih uzoraka integralnog pšeničnog brašna 14 je bilo kontaminirano (93,33%), a koncentracija DON-a kretala se u opsegu od 98 do 479 µg/kg. Maksimalna koncentracija DON-a (2.790 μg/kg) u ovom istraživanju zabeležena je kod pšeničnih mekinja. Prisustvo DON-a detektovano je u svih 15 ispitivanih uzorka pšeničnih mekinja (100%). Ovi rezultati ukazuju na visok procenat kontaminiranih uzoraka, pogotovo kod uzoraka pšeničnih mekinja, što predstavlja rizik po potrošače i iziskuje potrebu za monitoringom finalnih proizvoda pre puštanja u promet.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Presence of deoxynivalenol in wheat milling products in Serbia during 2016-2017, Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji",
number = "133",
pages = "57-62",
doi = "10.2298/ZMSPN1733057J"
}
Jauković, M., Zečević, V., Stanković, S.,& Krnjaja, V. (2017). Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji.
Zbornik Matice srpske za prirodne naukeMatica srpska, Novi Sad.(133), 57-62.
https://doi.org/10.2298/ZMSPN1733057J
Jauković M, Zečević V, Stanković S, Krnjaja V. Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji. Zbornik Matice srpske za prirodne nauke. 2017;(133):57-62
Jauković Marko, Zečević Veselinka, Stanković Slavica, Krnjaja Vesna, "Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji", no. 133 (2017):57-62,
https://doi.org/10.2298/ZMSPN1733057J .

Fungal contamination of maize grain samples with a special focus on toxigenic genera

Krnjaja, Vesna; Stanojković, Aleksandar; Stanković, Slavica; Lukić, Miloš; Bijelić, Zorica; Mandić, Violeta; Mićić, Nenad

(Institut za stočarstvo, Beograd, 2017)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanojković, Aleksandar
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Mićić, Nenad
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/664
AB  - In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi.
AB  - Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal contamination of maize grain samples with a special focus on toxigenic genera
T1  - Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove
VL  - 33
IS  - 2
SP  - 233
EP  - 241
DO  - 10.2298/BAH1702233K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanojković, Aleksandar and Stanković, Slavica and Lukić, Miloš and Bijelić, Zorica and Mandić, Violeta and Mićić, Nenad",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/664",
abstract = "In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi., Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal contamination of maize grain samples with a special focus on toxigenic genera, Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove",
volume = "33",
number = "2",
pages = "233-241",
doi = "10.2298/BAH1702233K"
}
Krnjaja, V., Stanojković, A., Stanković, S., Lukić, M., Bijelić, Z., Mandić, V.,& Mićić, N. (2017). Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 33(2), 233-241.
https://doi.org/10.2298/BAH1702233K
Krnjaja V, Stanojković A, Stanković S, Lukić M, Bijelić Z, Mandić V, Mićić N. Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove. Biotechnology in Animal Husbandry. 2017;33(2):233-241
Krnjaja Vesna, Stanojković Aleksandar, Stanković Slavica, Lukić Miloš, Bijelić Zorica, Mandić Violeta, Mićić Nenad, "Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove" 33, no. 2 (2017):233-241,
https://doi.org/10.2298/BAH1702233K .
4

Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels

Krstović, Saša Z.; Jakšić, Sandra M.; Bočarov-Stančić, Aleksandra; Stanković, Slavica; Janković, Snežana; Jajić, Igor M.

(Matica srpska, Novi Sad, 2017)

TY  - JOUR
AU  - Krstović, Saša Z.
AU  - Jakšić, Sandra M.
AU  - Bočarov-Stančić, Aleksandra
AU  - Stanković, Slavica
AU  - Janković, Snežana
AU  - Jajić, Igor M.
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/662
AB  - The production of fumonisins by potentially toxigenic Fusarium verticillioides isolates originating from Serbian maize and wheat kernels was tested in vitro. A total of six F. verticillioides isolates were incubated on yeast extract sucrose medium (YESA) for 4 weeks at 25 °C in the dark. Their toxin production potential was tested by applying a modified HPLC method for determination of fumonisins in cereals, since the TLC method gave no results. Analyses were performed on a HPLC-FLD system after sample extraction from YESA and extract clean-up on a SPE column. Although the isolates were tested for fumonisin B1, B2 and B3, only fumonisin B1 was detected. The results showed that all tested isolates had toxigenic potential for fumonisin B1 production. The average fumonisin B1 production of the isolates ranged from 7 to 289 µg/kg, thus indicating a highly variable toxigenic potential among the isolates. Isolate 1282 expressed the highest toxigenic potential for fumonisin B1 production (289 µg/kg), while isolate 2533/A showed a questionable potential for fumonisin production (7 µg/kg).
AB  - Proizvodnja fumonizina kod potencijalno toksikogenih Fusarium verticillioides izolata sa zrna kukuruza i pšenice poreklom iz Srbije testiran je in vitro. Ukupno šest izolata F. verticillioides inkubirano je na agarizovanoj podlozi sa ekstraktom kvasca i saharozom (YESA) tokom četiri nedelje, u mraku na 25 °C. Kapaciteti odabranih izolata za proizvodnju toksina detektovani su primenom modifikovane HPLC metode za određivanje fumonizina u zrnu žitarica, jer brza trijažna TLC metoda nije dala pozitivne rezultate. Analiza je izvedena na HPLC-FLD sistemu nakon ekstrakcije uzorka iz YESA kultura izolata i prečišćavanja ekstrakta na SPE koloni. Sve analize su urađene u tri ponavljanja. Iako su izolati F. verticillioides testirani na prisustvo fumonizina B1, B2 i B3, samo je fumonizin B1 bio detektovan. Rezultati su pokazali da svi testirani izolati imaju potencijal u sintezi fumonizina B1. Prosečna vrednost proizvedenog fumonizina B1 kod izolata F. verticillioides kretala se od 7 do 289 µg/kg, što ukazuje na izuzetno varijabilan toksigeni potencijal istih izolata. Izolat označen sa 1282 pokazao je najveći potencijal za biosintezu fumonizina B1 (289 µg/kg), dok je izolat 2533/A ispoljio diskutabilan potencijal za proizvodnju istog fumonizina (7 µg/kg).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels
T1  - Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije
IS  - 133
SP  - 71
EP  - 78
DO  - 10.2298/ZMSPN1733071K
ER  - 
@article{
author = "Krstović, Saša Z. and Jakšić, Sandra M. and Bočarov-Stančić, Aleksandra and Stanković, Slavica and Janković, Snežana and Jajić, Igor M.",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/662",
abstract = "The production of fumonisins by potentially toxigenic Fusarium verticillioides isolates originating from Serbian maize and wheat kernels was tested in vitro. A total of six F. verticillioides isolates were incubated on yeast extract sucrose medium (YESA) for 4 weeks at 25 °C in the dark. Their toxin production potential was tested by applying a modified HPLC method for determination of fumonisins in cereals, since the TLC method gave no results. Analyses were performed on a HPLC-FLD system after sample extraction from YESA and extract clean-up on a SPE column. Although the isolates were tested for fumonisin B1, B2 and B3, only fumonisin B1 was detected. The results showed that all tested isolates had toxigenic potential for fumonisin B1 production. The average fumonisin B1 production of the isolates ranged from 7 to 289 µg/kg, thus indicating a highly variable toxigenic potential among the isolates. Isolate 1282 expressed the highest toxigenic potential for fumonisin B1 production (289 µg/kg), while isolate 2533/A showed a questionable potential for fumonisin production (7 µg/kg)., Proizvodnja fumonizina kod potencijalno toksikogenih Fusarium verticillioides izolata sa zrna kukuruza i pšenice poreklom iz Srbije testiran je in vitro. Ukupno šest izolata F. verticillioides inkubirano je na agarizovanoj podlozi sa ekstraktom kvasca i saharozom (YESA) tokom četiri nedelje, u mraku na 25 °C. Kapaciteti odabranih izolata za proizvodnju toksina detektovani su primenom modifikovane HPLC metode za određivanje fumonizina u zrnu žitarica, jer brza trijažna TLC metoda nije dala pozitivne rezultate. Analiza je izvedena na HPLC-FLD sistemu nakon ekstrakcije uzorka iz YESA kultura izolata i prečišćavanja ekstrakta na SPE koloni. Sve analize su urađene u tri ponavljanja. Iako su izolati F. verticillioides testirani na prisustvo fumonizina B1, B2 i B3, samo je fumonizin B1 bio detektovan. Rezultati su pokazali da svi testirani izolati imaju potencijal u sintezi fumonizina B1. Prosečna vrednost proizvedenog fumonizina B1 kod izolata F. verticillioides kretala se od 7 do 289 µg/kg, što ukazuje na izuzetno varijabilan toksigeni potencijal istih izolata. Izolat označen sa 1282 pokazao je najveći potencijal za biosintezu fumonizina B1 (289 µg/kg), dok je izolat 2533/A ispoljio diskutabilan potencijal za proizvodnju istog fumonizina (7 µg/kg).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels, Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije",
number = "133",
pages = "71-78",
doi = "10.2298/ZMSPN1733071K"
}
Krstović, S. Z., Jakšić, S. M., Bočarov-Stančić, A., Stanković, S., Janković, S.,& Jajić, I. M. (2017). Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije.
Zbornik Matice srpske za prirodne naukeMatica srpska, Novi Sad.(133), 71-78.
https://doi.org/10.2298/ZMSPN1733071K
Krstović SZ, Jakšić SM, Bočarov-Stančić A, Stanković S, Janković S, Jajić IM. Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije. Zbornik Matice srpske za prirodne nauke. 2017;(133):71-78
Krstović Saša Z., Jakšić Sandra M., Bočarov-Stančić Aleksandra, Stanković Slavica, Janković Snežana, Jajić Igor M., "Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije", no. 133 (2017):71-78,
https://doi.org/10.2298/ZMSPN1733071K .

Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia

Obradović, Ana; Stanković, Slavica; Stevanović, Milan; Krnjaja, Vesna; Bulajić, Aleksandra; Delibašić, Goran

(Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Stevanović, Milan
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Delibašić, Goran
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/665
AB  - Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum.
AB  - Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia
T1  - Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji
VL  - 45
IS  - 3
SP  - 277
EP  - 286
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Stevanović, Milan and Krnjaja, Vesna and Bulajić, Aleksandra and Delibašić, Goran",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/665",
abstract = "Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum., Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia, Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji",
volume = "45",
number = "3",
pages = "277-286"
}
Obradović, A., Stanković, S., Stevanović, M., Krnjaja, V., Bulajić, A.,& Delibašić, G. (2017). Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji.
Biljni lekarUniverzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(3), 277-286.
Obradović A, Stanković S, Stevanović M, Krnjaja V, Bulajić A, Delibašić G. Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji. Biljni lekar. 2017;45(3):277-286
Obradović Ana, Stanković Slavica, Stevanović Milan, Krnjaja Vesna, Bulajić Aleksandra, Delibašić Goran, "Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji" 45, no. 3 (2017):277-286

Comparison of methods for determination of the toxigenic potential of Aspergillus parasiticus Sp. and Aspergillus flavus L. isolated from maize

Nikolić, Milica; Stanković, Slavica; Savić, Iva

(Matica srpska, Novi Sad, 2017)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Stanković, Slavica
AU  - Savić, Iva
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/666
AB  - Maize is considered one of the most susceptible crops to mycotoxins worldwide. Compared to other mycotoxins, the greatest attention has been paid to aflatoxins, due to their potential carcinogenicity and due to significant and longstanding problems they can cause in humans and animals. A. flavus and A. parasiticus produce aflatoxins in many economically significant crops in both fields and storages. Because of the potential aflatoxin contamination of maize grain, the toxigenic potential of A. flavus and A. parasiticus isolates, originating from Serbia, was tested in the present study. Furthermore, various applied methods for detection of these mycotoxins were compared in the study. Cultural, serological and analytical methods for the detection of mycotoxins were compared in the course of the experiment by the direct extraction of aflatoxins from the nutrient medium. The cultural methods for the detection of aflatoxin production were applied to 20 isolates of A. flavus (MRIZP Af18-20) and A. parasiticus (MRIZP Ap1-17). These methods are based on the yellow pigment formation in mycelia and nutrition media, occurrence of fluorescence on PDA (potato dextrose agar), agar containing β-cyclodextrine (CD-PDA), as well as on the red pigment formation after adding ammonium hydroxide to the existing medium. The ELISA was used to check quantitative and qualitative analyses of total aflatoxins (B1, B2, G1, G2) while the HPLC method was applied to establish ability of isolates to synthesize aflatoxins B1, B2, G1, G2. The yellow pigment formation, fluorescence and colony color changes of isolates into red, as a proof of toxigenicity of isolates, were confirmed in all cases by ELISA. A high potential of total aflatoxin production was determined in the majority of observed isolates. The ability of A. parasiticus isolates to synthesize aflatoxins G1 and G2 was confirmed by the HPLC method. This was essential for a better understanding of the key role of the suitability of cultural methods for preliminary evaluation of a large number of isolates. Our goal was to employ rapid biochemical approaches to prevent aflatoxin contamination of crops, and to reduce human and animal exposure to foodborne mycotoxins.
AB  - Kukuruz se širom sveta smatra jednim od useva najpodložnijih za kontaminaciju mikotoksinima. Aflatoksinima se, u poređenju s drugim mikotoksinima, pridaje najveća pažnja zbog njihove potencijalne kancerogenosti, značajnih i dugoročnih problema koje izazivaju kod ljudi i životinja. Vrste A. flavus i A. parasiticus mogu produkovati aflatoksine kod mnogih ekonomski značajnih kultura u poljima i skladištima. Zbog potencijalne kontaminacije zrna kukuruza aflatoksinima u ovom radu je ispitan toksigeni potencijal izolata upravo ove dve vrste poreklom iz Srbije i upoređene su različite metode detekcije ovih mikotoksina. Tokom eksperimenta upoređene su odgajivačke, serološke i analitičke metode detekcije mikotoksina, direktnom ekstrakcijom aflatoksina iz hranljive podloge. Istraživanja su bazirana na primeni odgajivačke metode za određivanje produkcije aflatoksina kod 20 izolata A. parasiticus (MRIZP Ap1-17) i A. flavus (MRIZP Af18-20) poreklom iz Srbije. Odgajivačke metode su bile zasnovane na formiranju žutog pigmenta u miceliji i hranljivoj podlozi, na pojavi fluorescencije na PDA (krompir dekstrozni agar) i podlozi koja sadrzi β-ciklodekstrin (CD-PDA), kao i na obrazovanju crvenog pigmenta u podlozi nakon dodavanja amonijum hidroksida. ELISA test je korišćen za proveru kvantitativnih i kvalitativnih sadržaja ukupnih aflatoksina B1, B2, G1 i G 2, dok je HPLC metodom utvrđena koncentracija pojedinačnih aflatoksina B1, B2, G1 i G2. Obrazovanje žutog pigmenta, fluorescencija i promena boje kolonije izolata u crvenu, kao dokaz toksigenosti izolata, potvrđena je u svim slučajevima i ELISA testom. Kod većine izolata ustanovljen je visok potencijal produkcije ukupnih aflatoksina. HPLC metodom potvrđena je i sposobnost sinteze aflatoksina G1 i G2 od strane izolata A. parasiticus. Cilj eksperimenta bio je da se ispita efikasnost upotrebe brzih testova za detekciju aflatoksina, kako bi se sprečila kontaminacija useva i izloženost ljudi i životinja afla-toksinima.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Comparison of methods for determination of the toxigenic potential of Aspergillus parasiticus Sp. and Aspergillus flavus L. isolated from maize
T1  - Komparacija metoda za utvrđivanje toksigenog potencijala Aspergillus parasiticus Speare i Aspergillus flavus Link izolovanih sa kukuruza
IS  - 133
SP  - 95
EP  - 104
DO  - 10.2298/ZMSPN1733095N
ER  - 
@article{
author = "Nikolić, Milica and Stanković, Slavica and Savić, Iva",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/666",
abstract = "Maize is considered one of the most susceptible crops to mycotoxins worldwide. Compared to other mycotoxins, the greatest attention has been paid to aflatoxins, due to their potential carcinogenicity and due to significant and longstanding problems they can cause in humans and animals. A. flavus and A. parasiticus produce aflatoxins in many economically significant crops in both fields and storages. Because of the potential aflatoxin contamination of maize grain, the toxigenic potential of A. flavus and A. parasiticus isolates, originating from Serbia, was tested in the present study. Furthermore, various applied methods for detection of these mycotoxins were compared in the study. Cultural, serological and analytical methods for the detection of mycotoxins were compared in the course of the experiment by the direct extraction of aflatoxins from the nutrient medium. The cultural methods for the detection of aflatoxin production were applied to 20 isolates of A. flavus (MRIZP Af18-20) and A. parasiticus (MRIZP Ap1-17). These methods are based on the yellow pigment formation in mycelia and nutrition media, occurrence of fluorescence on PDA (potato dextrose agar), agar containing β-cyclodextrine (CD-PDA), as well as on the red pigment formation after adding ammonium hydroxide to the existing medium. The ELISA was used to check quantitative and qualitative analyses of total aflatoxins (B1, B2, G1, G2) while the HPLC method was applied to establish ability of isolates to synthesize aflatoxins B1, B2, G1, G2. The yellow pigment formation, fluorescence and colony color changes of isolates into red, as a proof of toxigenicity of isolates, were confirmed in all cases by ELISA. A high potential of total aflatoxin production was determined in the majority of observed isolates. The ability of A. parasiticus isolates to synthesize aflatoxins G1 and G2 was confirmed by the HPLC method. This was essential for a better understanding of the key role of the suitability of cultural methods for preliminary evaluation of a large number of isolates. Our goal was to employ rapid biochemical approaches to prevent aflatoxin contamination of crops, and to reduce human and animal exposure to foodborne mycotoxins., Kukuruz se širom sveta smatra jednim od useva najpodložnijih za kontaminaciju mikotoksinima. Aflatoksinima se, u poređenju s drugim mikotoksinima, pridaje najveća pažnja zbog njihove potencijalne kancerogenosti, značajnih i dugoročnih problema koje izazivaju kod ljudi i životinja. Vrste A. flavus i A. parasiticus mogu produkovati aflatoksine kod mnogih ekonomski značajnih kultura u poljima i skladištima. Zbog potencijalne kontaminacije zrna kukuruza aflatoksinima u ovom radu je ispitan toksigeni potencijal izolata upravo ove dve vrste poreklom iz Srbije i upoređene su različite metode detekcije ovih mikotoksina. Tokom eksperimenta upoređene su odgajivačke, serološke i analitičke metode detekcije mikotoksina, direktnom ekstrakcijom aflatoksina iz hranljive podloge. Istraživanja su bazirana na primeni odgajivačke metode za određivanje produkcije aflatoksina kod 20 izolata A. parasiticus (MRIZP Ap1-17) i A. flavus (MRIZP Af18-20) poreklom iz Srbije. Odgajivačke metode su bile zasnovane na formiranju žutog pigmenta u miceliji i hranljivoj podlozi, na pojavi fluorescencije na PDA (krompir dekstrozni agar) i podlozi koja sadrzi β-ciklodekstrin (CD-PDA), kao i na obrazovanju crvenog pigmenta u podlozi nakon dodavanja amonijum hidroksida. ELISA test je korišćen za proveru kvantitativnih i kvalitativnih sadržaja ukupnih aflatoksina B1, B2, G1 i G 2, dok je HPLC metodom utvrđena koncentracija pojedinačnih aflatoksina B1, B2, G1 i G2. Obrazovanje žutog pigmenta, fluorescencija i promena boje kolonije izolata u crvenu, kao dokaz toksigenosti izolata, potvrđena je u svim slučajevima i ELISA testom. Kod većine izolata ustanovljen je visok potencijal produkcije ukupnih aflatoksina. HPLC metodom potvrđena je i sposobnost sinteze aflatoksina G1 i G2 od strane izolata A. parasiticus. Cilj eksperimenta bio je da se ispita efikasnost upotrebe brzih testova za detekciju aflatoksina, kako bi se sprečila kontaminacija useva i izloženost ljudi i životinja afla-toksinima.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Comparison of methods for determination of the toxigenic potential of Aspergillus parasiticus Sp. and Aspergillus flavus L. isolated from maize, Komparacija metoda za utvrđivanje toksigenog potencijala Aspergillus parasiticus Speare i Aspergillus flavus Link izolovanih sa kukuruza",
number = "133",
pages = "95-104",
doi = "10.2298/ZMSPN1733095N"
}
Nikolić, M., Stanković, S.,& Savić, I. (2017). Komparacija metoda za utvrđivanje toksigenog potencijala Aspergillus parasiticus Speare i Aspergillus flavus Link izolovanih sa kukuruza.
Zbornik Matice srpske za prirodne naukeMatica srpska, Novi Sad.(133), 95-104.
https://doi.org/10.2298/ZMSPN1733095N
Nikolić M, Stanković S, Savić I. Komparacija metoda za utvrđivanje toksigenog potencijala Aspergillus parasiticus Speare i Aspergillus flavus Link izolovanih sa kukuruza. Zbornik Matice srpske za prirodne nauke. 2017;(133):95-104
Nikolić Milica, Stanković Slavica, Savić Iva, "Komparacija metoda za utvrđivanje toksigenog potencijala Aspergillus parasiticus Speare i Aspergillus flavus Link izolovanih sa kukuruza", no. 133 (2017):95-104,
https://doi.org/10.2298/ZMSPN1733095N .

Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest

Bijelić, Zorica; Krnjaja, Vesna; Muslic-Ruzić, Dragana; Mandić, Violeta; Škrbić, Zdenka; Lukić, Miloš; Stanković, Slavica

(Ars Docendi, Bucharest, 2017)

TY  - JOUR
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Muslic-Ruzić, Dragana
AU  - Mandić, Violeta
AU  - Škrbić, Zdenka
AU  - Lukić, Miloš
AU  - Stanković, Slavica
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/688
AB  - The aim of this study was to determine the presence of fungi and concentrations of mycotoxins, zearalenone, fumonisin and deoxynivalenol, in lucerne and grass-legume silages, under the influence of different doses of N fertilization and harvesting at different stages of phenological development of the plants. Studies included pure lucerne crop and mixtures of lucerne with cocksfoot, tall fescue and sainfoin, sown in different ratios, fertilized with 0, 70, 140 or 210 kgN ha(-1) and harvested in the butonization phase or at 50% flowering of lucerne plants. Results showed that the total fungi count in the silage depended on the three investigated factors. The highest total fungi count was determined in the mixture silages of lucerne, cocksfoot and tall fescue, which was well treated with different nitrogen quantities, harvested and prepared in later stages of plant development. The most commonly-occurring fungi were Fusarium species (85.5% of fungi were this genus). Of the studied mycotoxins, only DON depended on fertilization, as it reduced the concentration of DON from 0.15 to 0.07 mg kg(-1). Preventing appearance of fungi and their mycotoxins in forage and silage should begin in the field as well as during the process of preparation of silage.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest
VL  - 22
IS  - 5
SP  - 12907
EP  - 12914
ER  - 
@article{
author = "Bijelić, Zorica and Krnjaja, Vesna and Muslic-Ruzić, Dragana and Mandić, Violeta and Škrbić, Zdenka and Lukić, Miloš and Stanković, Slavica",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/688",
abstract = "The aim of this study was to determine the presence of fungi and concentrations of mycotoxins, zearalenone, fumonisin and deoxynivalenol, in lucerne and grass-legume silages, under the influence of different doses of N fertilization and harvesting at different stages of phenological development of the plants. Studies included pure lucerne crop and mixtures of lucerne with cocksfoot, tall fescue and sainfoin, sown in different ratios, fertilized with 0, 70, 140 or 210 kgN ha(-1) and harvested in the butonization phase or at 50% flowering of lucerne plants. Results showed that the total fungi count in the silage depended on the three investigated factors. The highest total fungi count was determined in the mixture silages of lucerne, cocksfoot and tall fescue, which was well treated with different nitrogen quantities, harvested and prepared in later stages of plant development. The most commonly-occurring fungi were Fusarium species (85.5% of fungi were this genus). Of the studied mycotoxins, only DON depended on fertilization, as it reduced the concentration of DON from 0.15 to 0.07 mg kg(-1). Preventing appearance of fungi and their mycotoxins in forage and silage should begin in the field as well as during the process of preparation of silage.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest",
volume = "22",
number = "5",
pages = "12907-12914"
}
Bijelić, Z., Krnjaja, V., Muslic-Ruzić, D., Mandić, V., Škrbić, Z., Lukić, M.,& Stanković, S. (2017). Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest.
Romanian Biotechnological LettersArs Docendi, Bucharest., 22(5), 12907-12914.
Bijelić Z, Krnjaja V, Muslic-Ruzić D, Mandić V, Škrbić Z, Lukić M, Stanković S. Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest. Romanian Biotechnological Letters. 2017;22(5):12907-12914
Bijelić Zorica, Krnjaja Vesna, Muslic-Ruzić Dragana, Mandić Violeta, Škrbić Zdenka, Lukić Miloš, Stanković Slavica, "Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest" 22, no. 5 (2017):12907-12914
1

Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt

Jauković, Marko; Zečević, Veselinka; Stanković, Slavica; Krnjaja, Vesna; Nikić, Tanja S.; Bailović, Stanislava M.; Tadić, Jelena I.

(Amer Soc Brewing Chemists Inc, St Paul, 2017)

TY  - JOUR
AU  - Jauković, Marko
AU  - Zečević, Veselinka
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Nikić, Tanja S.
AU  - Bailović, Stanislava M.
AU  - Tadić, Jelena I.
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/687
AB  - The primary aim of this research was to investigate the effect of dilute alkaline steeping on molds and toxicity of maize malt. Samples of maize were collected from one farm located in the Serbian autonomous region Vojvodina. Steeping regimes were performed by using 0.1, 0.2, and 0.3% NaOH solutions. The effects of these solutions on mold contamination, total aflatoxin (AFLA), deoxynivalenol (DON), zearalenone (ZON), rootlet length, moisture, total nitrogen, content of protein, fat content, ash content, content of total carbohydrates, and energy value of maize malt were evaluated. Steeping in 0.1, 0.2, and 0.3% NaOH reduced the levels of molds from 10(6) to 10(4) CFU/g, and steeping in 0.3% NaOH eliminated some of the mold genera. Also, it significantly reduced (P  lt  0.05) the level of total AFLA, DON, and ZON. However, steeping in (control) water also significantly reduced (P  lt  0.05) the level of DON and ZON. Malting also significantly reduced (P  lt  0.05) total nitrogen and therefore content of proteins. However, steeping in 0.1, 0.2, and 0.3% NaOH caused significantly smaller reductions (P  lt  0.05). Significant reduction (P  lt  0.05) was also observed in the rootlet length, content of fat, and ash content. Steeping in 0.3% NaOH is proposed as a method for the reduction of mold and AFLA, DON, and ZON contamination during maize malting. Energy value of maize increases in the malting process regardless of the steeping regime.
PB  - Amer Soc Brewing Chemists Inc, St Paul
T2  - Journal of the American Society of Brewing Chemists
T1  - Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt
VL  - 75
IS  - 4
SP  - 369
EP  - 373
DO  - 10.1094/ASBCJ-2017-4043-01
ER  - 
@article{
author = "Jauković, Marko and Zečević, Veselinka and Stanković, Slavica and Krnjaja, Vesna and Nikić, Tanja S. and Bailović, Stanislava M. and Tadić, Jelena I.",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/687",
abstract = "The primary aim of this research was to investigate the effect of dilute alkaline steeping on molds and toxicity of maize malt. Samples of maize were collected from one farm located in the Serbian autonomous region Vojvodina. Steeping regimes were performed by using 0.1, 0.2, and 0.3% NaOH solutions. The effects of these solutions on mold contamination, total aflatoxin (AFLA), deoxynivalenol (DON), zearalenone (ZON), rootlet length, moisture, total nitrogen, content of protein, fat content, ash content, content of total carbohydrates, and energy value of maize malt were evaluated. Steeping in 0.1, 0.2, and 0.3% NaOH reduced the levels of molds from 10(6) to 10(4) CFU/g, and steeping in 0.3% NaOH eliminated some of the mold genera. Also, it significantly reduced (P  lt  0.05) the level of total AFLA, DON, and ZON. However, steeping in (control) water also significantly reduced (P  lt  0.05) the level of DON and ZON. Malting also significantly reduced (P  lt  0.05) total nitrogen and therefore content of proteins. However, steeping in 0.1, 0.2, and 0.3% NaOH caused significantly smaller reductions (P  lt  0.05). Significant reduction (P  lt  0.05) was also observed in the rootlet length, content of fat, and ash content. Steeping in 0.3% NaOH is proposed as a method for the reduction of mold and AFLA, DON, and ZON contamination during maize malting. Energy value of maize increases in the malting process regardless of the steeping regime.",
publisher = "Amer Soc Brewing Chemists Inc, St Paul",
journal = "Journal of the American Society of Brewing Chemists",
title = "Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt",
volume = "75",
number = "4",
pages = "369-373",
doi = "10.1094/ASBCJ-2017-4043-01"
}
Jauković, M., Zečević, V., Stanković, S., Krnjaja, V., Nikić, T. S., Bailović, S. M.,& Tadić, J. I. (2017). Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt.
Journal of the American Society of Brewing ChemistsAmer Soc Brewing Chemists Inc, St Paul., 75(4), 369-373.
https://doi.org/10.1094/ASBCJ-2017-4043-01
Jauković M, Zečević V, Stanković S, Krnjaja V, Nikić TS, Bailović SM, Tadić JI. Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt. Journal of the American Society of Brewing Chemists. 2017;75(4):369-373
Jauković Marko, Zečević Veselinka, Stanković Slavica, Krnjaja Vesna, Nikić Tanja S., Bailović Stanislava M., Tadić Jelena I., "Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt" 75, no. 4 (2017):369-373,
https://doi.org/10.1094/ASBCJ-2017-4043-01 .
1
1
1

A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes

Pasquali, Matias; Beyer, Marco; Hoffmann, Lucien; Pallez-Barthel, Marine; Piec, Jonathan; Logrieco, Antonio; Moretti, Antonio; Audenaert, Kris; Vanheule, Adriaan; Balmas, Virgilio; Migheli, Quirico; Scherm, Barbara; Basler, Ryan; Boutigny, Anne-Laure; Chrpova, Jana; Czembor, Elzbieta; Gagkaeva, Tatiana; Gonzalez-Jaen, Maria; Marin, Patricia; Hofgaard, Ingerd S.; Koycu, Nagehan D.; Lević, Jelena; Stanković, Slavica; Miedaner, Thomas; Mueller, Marina E. H.; Munaut, Francoise; Scauflaire, Jonathan; Parikka, Paivi; Thrane, Ulf; Uhlig, Silvio; Yli-Mattila, Tapani; Vogelgsang, Susanne

(Frontiers Media Sa, Lausanne, 2016)

TY  - JOUR
AU  - Pasquali, Matias
AU  - Beyer, Marco
AU  - Hoffmann, Lucien
AU  - Pallez-Barthel, Marine
AU  - Piec, Jonathan
AU  - Logrieco, Antonio
AU  - Moretti, Antonio
AU  - Audenaert, Kris
AU  - Vanheule, Adriaan
AU  - Balmas, Virgilio
AU  - Migheli, Quirico
AU  - Scherm, Barbara
AU  - Basler, Ryan
AU  - Boutigny, Anne-Laure
AU  - Chrpova, Jana
AU  - Czembor, Elzbieta
AU  - Gagkaeva, Tatiana
AU  - Gonzalez-Jaen, Maria
AU  - Marin, Patricia
AU  - Hofgaard, Ingerd S.
AU  - Koycu, Nagehan D.
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Miedaner, Thomas
AU  - Mueller, Marina E. H.
AU  - Munaut, Francoise
AU  - Scauflaire, Jonathan
AU  - Parikka, Paivi
AU  - Thrane, Ulf
AU  - Uhlig, Silvio
AU  - Yli-Mattila, Tapani
AU  - Vogelgsang, Susanne
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/655
AB  - Fusarium species, particularly Fusarium graminearum and F culmorum, are the main cause of trichothecene type B contamination in cereals. Data on the distribution of Fusarium trichothecene genotypes in cereals in Europe are scattered in time and space. Furthermore, a common core set of related variables (sampling method, host cultivar, previous crop, etc.) that would allow more effective analysis of factors influencing the spatial and temporal population distribution, is lacking. Consequently, based on the available data, it is difficult to identify factors influencing chemotype distribution and spread at the European level. Here we describe the results of a collaborative integrated work which aims (1) to characterize the trichothecene genotypes of strains from three Fusarium species, collected over the period 2000-2013 and (2) to enhance the standardization of epidemiological data collection. Information on host plant, country of origin, sampling location, year of sampling and previous crop of 1147 F graminearurn, 479 F culmorum, and 3 F cortaderiae strains obtained from 17 European countries was compiled and a map of trichothecene type B genotype distribution was plotted for each species. All information on the strains was collected in a freely accessible and updatable database (www.catalogueeu.luxmcc.lu), which will serve as a starting point for epidemiological analysis of potential spatial and temporal trichothecene genotype shifts in Europe. The analysis of the currently available European dataset showed that in F. grarninearum, the predominant genotype was 15-acetyldeoxynivalenol (15-ADON) (82.9%), followed by 3-acetyldeoxynivalenol (3-ADON) (13.6%), and nivalenol (NIV) (3.5%). In F culmorum, the prevalent genotype was 3-ADON (59.9%), while the NIV genotype accounted for the remaining 40.1%. Both, geographical and temporal patterns of trichothecene genotypes distribution were identified.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes
VL  - 7
DO  - 10.3389/fmicb.2016.00406
ER  - 
@article{
author = "Pasquali, Matias and Beyer, Marco and Hoffmann, Lucien and Pallez-Barthel, Marine and Piec, Jonathan and Logrieco, Antonio and Moretti, Antonio and Audenaert, Kris and Vanheule, Adriaan and Balmas, Virgilio and Migheli, Quirico and Scherm, Barbara and Basler, Ryan and Boutigny, Anne-Laure and Chrpova, Jana and Czembor, Elzbieta and Gagkaeva, Tatiana and Gonzalez-Jaen, Maria and Marin, Patricia and Hofgaard, Ingerd S. and Koycu, Nagehan D. and Lević, Jelena and Stanković, Slavica and Miedaner, Thomas and Mueller, Marina E. H. and Munaut, Francoise and Scauflaire, Jonathan and Parikka, Paivi and Thrane, Ulf and Uhlig, Silvio and Yli-Mattila, Tapani and Vogelgsang, Susanne",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/655",
abstract = "Fusarium species, particularly Fusarium graminearum and F culmorum, are the main cause of trichothecene type B contamination in cereals. Data on the distribution of Fusarium trichothecene genotypes in cereals in Europe are scattered in time and space. Furthermore, a common core set of related variables (sampling method, host cultivar, previous crop, etc.) that would allow more effective analysis of factors influencing the spatial and temporal population distribution, is lacking. Consequently, based on the available data, it is difficult to identify factors influencing chemotype distribution and spread at the European level. Here we describe the results of a collaborative integrated work which aims (1) to characterize the trichothecene genotypes of strains from three Fusarium species, collected over the period 2000-2013 and (2) to enhance the standardization of epidemiological data collection. Information on host plant, country of origin, sampling location, year of sampling and previous crop of 1147 F graminearurn, 479 F culmorum, and 3 F cortaderiae strains obtained from 17 European countries was compiled and a map of trichothecene type B genotype distribution was plotted for each species. All information on the strains was collected in a freely accessible and updatable database (www.catalogueeu.luxmcc.lu), which will serve as a starting point for epidemiological analysis of potential spatial and temporal trichothecene genotype shifts in Europe. The analysis of the currently available European dataset showed that in F. grarninearum, the predominant genotype was 15-acetyldeoxynivalenol (15-ADON) (82.9%), followed by 3-acetyldeoxynivalenol (3-ADON) (13.6%), and nivalenol (NIV) (3.5%). In F culmorum, the prevalent genotype was 3-ADON (59.9%), while the NIV genotype accounted for the remaining 40.1%. Both, geographical and temporal patterns of trichothecene genotypes distribution were identified.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes",
volume = "7",
doi = "10.3389/fmicb.2016.00406"
}
Pasquali, M., Beyer, M., Hoffmann, L., Pallez-Barthel, M., Piec, J., Logrieco, A., Moretti, A., Audenaert, K., Vanheule, A., Balmas, V., Migheli, Q., Scherm, B., Basler, R., Boutigny, A., Chrpova, J., Czembor, E., Gagkaeva, T., Gonzalez-Jaen, M., Marin, P., Hofgaard, I. S., Koycu, N. D., Lević, J., Stanković, S., Miedaner, T., Mueller, M. E. H., Munaut, F., Scauflaire, J., Parikka, P., Thrane, U., Uhlig, S., Yli-Mattila, T.,& Vogelgsang, S. (2016). A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes.
Frontiers in MicrobiologyFrontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmicb.2016.00406
Pasquali M, Beyer M, Hoffmann L, Pallez-Barthel M, Piec J, Logrieco A, Moretti A, Audenaert K, Vanheule A, Balmas V, Migheli Q, Scherm B, Basler R, Boutigny A, Chrpova J, Czembor E, Gagkaeva T, Gonzalez-Jaen M, Marin P, Hofgaard IS, Koycu ND, Lević J, Stanković S, Miedaner T, Mueller MEH, Munaut F, Scauflaire J, Parikka P, Thrane U, Uhlig S, Yli-Mattila T, Vogelgsang S. A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes. Frontiers in Microbiology. 2016;7
Pasquali Matias, Beyer Marco, Hoffmann Lucien, Pallez-Barthel Marine, Piec Jonathan, Logrieco Antonio, Moretti Antonio, Audenaert Kris, Vanheule Adriaan, Balmas Virgilio, Migheli Quirico, Scherm Barbara, Basler Ryan, Boutigny Anne-Laure, Chrpova Jana, Czembor Elzbieta, Gagkaeva Tatiana, Gonzalez-Jaen Maria, Marin Patricia, Hofgaard Ingerd S., Koycu Nagehan D., Lević Jelena, Stanković Slavica, Miedaner Thomas, Mueller Marina E. H., Munaut Francoise, Scauflaire Jonathan, Parikka Paivi, Thrane Ulf, Uhlig Silvio, Yli-Mattila Tapani, Vogelgsang Susanne, "A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes" 7 (2016),
https://doi.org/10.3389/fmicb.2016.00406 .
4
70
64
72

Pathogenicity of Aspergillus spp. isolates originating from Serbia

Nikolić, Milica; Savić, Iva; Stanković, Slavica

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2016)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Stanković, Slavica
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/645
AB  - Species of the genus Aspergillus, section Flavi are facultative plant pathogens with the capability to contaminate primary agricultural products in the field, at harvest, in warehouses, and during processing. Infection is done through silk or injuries on maize ears. The objective of this study was to test pathogenicity of 25 A. parasiticus isolates on barley leaves. A parasiticus was isolated from maize kernels collected from 22 locations in Serbia during 2013-2015. Inoculation of the first barley leaf was done by the spore suspension of the fungus A. parasiticus. The spot sizes were statistically compared and this comparison showed that the isolate MRIZP 3930 was the least virulent. The isolate MRIZP 3819 was the most virulent seven days after inoculation and together with the isolate MRIZP 4265 was classified into the most aggressive group rated as 5. Pathogenicity was confirmed in all isolates.
AB  - Vrste roda Aspergillus sekcije Flavi su fakultativní biljni patogeni, čija se sposobnost ogleda u kontaminaciji primarnih poljoprivrednih proizvoda u polju, tokom berbe, u skladištu i tokom procesa prerade. Infekcija se obavlja preko svile ili oštećenja na klipu kukuruza. Cilj ovog rada bio je da se oceni patogenost 25 izolata A. parasiticus na listovima ječma. Kulture A. parasiticus su izolovane iz uzoraka zrna kukuruza koji su prikupljani sa 22 lokaliteta u Srbiji u periodu 2013-2015. godina. Inokulacija prvog lista ječma izvršena je pomoću suspenzije spora gljive A. parasiticus. Statističkim poređenjem dobijenih vrednosti veličina pega, pokazano je da je najmanje virulentan izolat MRIZP 3930. Izolat MRIZP 3819 se izvojio kao najvirulentniji posle 7 dana inokulacije, a zajedno s izolatom MRIZP 4265 je svrstan u najagresivniju grupu koja je kategorisana ocenom 5. Svi ispitivani izolati su potvrdili patogenost.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Pathogenicity of Aspergillus spp. isolates originating from Serbia
T1  - Patogenost izolata Aspergillus spp. poreklom iz Srbije
VL  - 53
IS  - 3
SP  - 101
EP  - 105
DO  - 10.5937/ratpov53-10795
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Stanković, Slavica",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/645",
abstract = "Species of the genus Aspergillus, section Flavi are facultative plant pathogens with the capability to contaminate primary agricultural products in the field, at harvest, in warehouses, and during processing. Infection is done through silk or injuries on maize ears. The objective of this study was to test pathogenicity of 25 A. parasiticus isolates on barley leaves. A parasiticus was isolated from maize kernels collected from 22 locations in Serbia during 2013-2015. Inoculation of the first barley leaf was done by the spore suspension of the fungus A. parasiticus. The spot sizes were statistically compared and this comparison showed that the isolate MRIZP 3930 was the least virulent. The isolate MRIZP 3819 was the most virulent seven days after inoculation and together with the isolate MRIZP 4265 was classified into the most aggressive group rated as 5. Pathogenicity was confirmed in all isolates., Vrste roda Aspergillus sekcije Flavi su fakultativní biljni patogeni, čija se sposobnost ogleda u kontaminaciji primarnih poljoprivrednih proizvoda u polju, tokom berbe, u skladištu i tokom procesa prerade. Infekcija se obavlja preko svile ili oštećenja na klipu kukuruza. Cilj ovog rada bio je da se oceni patogenost 25 izolata A. parasiticus na listovima ječma. Kulture A. parasiticus su izolovane iz uzoraka zrna kukuruza koji su prikupljani sa 22 lokaliteta u Srbiji u periodu 2013-2015. godina. Inokulacija prvog lista ječma izvršena je pomoću suspenzije spora gljive A. parasiticus. Statističkim poređenjem dobijenih vrednosti veličina pega, pokazano je da je najmanje virulentan izolat MRIZP 3930. Izolat MRIZP 3819 se izvojio kao najvirulentniji posle 7 dana inokulacije, a zajedno s izolatom MRIZP 4265 je svrstan u najagresivniju grupu koja je kategorisana ocenom 5. Svi ispitivani izolati su potvrdili patogenost.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Pathogenicity of Aspergillus spp. isolates originating from Serbia, Patogenost izolata Aspergillus spp. poreklom iz Srbije",
volume = "53",
number = "3",
pages = "101-105",
doi = "10.5937/ratpov53-10795"
}
Nikolić, M., Savić, I.,& Stanković, S. (2016). Patogenost izolata Aspergillus spp. poreklom iz Srbije.
Ratarstvo i povrtarstvoInstitut za ratarstvo i povrtarstvo, Novi Sad., 53(3), 101-105.
https://doi.org/10.5937/ratpov53-10795
Nikolić M, Savić I, Stanković S. Patogenost izolata Aspergillus spp. poreklom iz Srbije. Ratarstvo i povrtarstvo. 2016;53(3):101-105
Nikolić Milica, Savić Iva, Stanković Slavica, "Patogenost izolata Aspergillus spp. poreklom iz Srbije" 53, no. 3 (2016):101-105,
https://doi.org/10.5937/ratpov53-10795 .
1

Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover

Vasić, Tanja; Terzić, Dragan; Milenković, Jasmina; Marković, Jordan; Krnjaja, Vesna; Jevremović, Darko; Stanković, Slavica

(Društvo genetičara Srbije, Beograd, 2016)

TY  - JOUR
AU  - Vasić, Tanja
AU  - Terzić, Dragan
AU  - Milenković, Jasmina
AU  - Marković, Jordan
AU  - Krnjaja, Vesna
AU  - Jevremović, Darko
AU  - Stanković, Slavica
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/650
AB  - A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover
VL  - 48
IS  - 1
SP  - 187
EP  - 198
DO  - 10.2298/GENSR1601187V
ER  - 
@article{
author = "Vasić, Tanja and Terzić, Dragan and Milenković, Jasmina and Marković, Jordan and Krnjaja, Vesna and Jevremović, Darko and Stanković, Slavica",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/650",
abstract = "A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover",
volume = "48",
number = "1",
pages = "187-198",
doi = "10.2298/GENSR1601187V"
}
Vasić, T., Terzić, D., Milenković, J., Marković, J., Krnjaja, V., Jevremović, D.,& Stanković, S. (2016). Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover.
GenetikaDruštvo genetičara Srbije, Beograd., 48(1), 187-198.
https://doi.org/10.2298/GENSR1601187V
Vasić T, Terzić D, Milenković J, Marković J, Krnjaja V, Jevremović D, Stanković S. Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. Genetika. 2016;48(1):187-198
Vasić Tanja, Terzić Dragan, Milenković Jasmina, Marković Jordan, Krnjaja Vesna, Jevremović Darko, Stanković Slavica, "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover" 48, no. 1 (2016):187-198,
https://doi.org/10.2298/GENSR1601187V .

Fungal and mycotoxin contamination of maize hybrids in different maturity groups

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Bijelić, Zorica; Vasić, Tanja; Jauković, Marko

(Institut za stočarstvo, Beograd, 2016)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Vasić, Tanja
AU  - Jauković, Marko
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/627
AB  - In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods.
AB  - U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal and mycotoxin contamination of maize hybrids in different maturity groups
T1  - Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja
VL  - 32
IS  - 1
SP  - 71
EP  - 81
DO  - 10.2298/BAH1601071K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Vasić, Tanja and Jauković, Marko",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/627",
abstract = "In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods., U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal and mycotoxin contamination of maize hybrids in different maturity groups, Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja",
volume = "32",
number = "1",
pages = "71-81",
doi = "10.2298/BAH1601071K"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Bijelić, Z., Vasić, T.,& Jauković, M. (2016). Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 32(1), 71-81.
https://doi.org/10.2298/BAH1601071K
Krnjaja V, Stanković S, Obradović A, Mandić V, Bijelić Z, Vasić T, Jauković M. Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja. Biotechnology in Animal Husbandry. 2016;32(1):71-81
Krnjaja Vesna, Stanković Slavica, Obradović Ana, Mandić Violeta, Bijelić Zorica, Vasić Tanja, Jauković Marko, "Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja" 32, no. 1 (2016):71-81,
https://doi.org/10.2298/BAH1601071K .
4

Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain

Tančić, Sonja; Stanković, Slavica; Lević, Jelena; Krnjaja, Vesna

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2015)

TY  - JOUR
AU  - Tančić, Sonja
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/607
AB  - A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp.
AB  - Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain
T1  - Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza
VL  - 30
IS  - 2
SP  - 99
EP  - 105
DO  - 10.2298/pif1502099T
ER  - 
@article{
author = "Tančić, Sonja and Stanković, Slavica and Lević, Jelena and Krnjaja, Vesna",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/607",
abstract = "A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp., Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain, Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza",
volume = "30",
number = "2",
pages = "99-105",
doi = "10.2298/pif1502099T"
}
Tančić, S., Stanković, S., Lević, J.,& Krnjaja, V. (2015). Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza.
Pesticidi i fitomedicinaInstitut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 30(2), 99-105.
https://doi.org/10.2298/pif1502099T
Tančić S, Stanković S, Lević J, Krnjaja V. Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza. Pesticidi i fitomedicina. 2015;30(2):99-105
Tančić Sonja, Stanković Slavica, Lević Jelena, Krnjaja Vesna, "Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza" 30, no. 2 (2015):99-105,
https://doi.org/10.2298/pif1502099T .
6

Fusarium infection and deoxynivalenol contamination in winter wheat

Krnjaja, Vesna; Tomić, Z.; Stanković, Slavica; Petrović, T.; Bijelić, Zorica; Mandić, Violeta; Obradović, Ana

(Institut za stočarstvo, Beograd, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Tomić, Z.
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Obradović, Ana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/606
AB  - In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat.
AB  - U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fusarium infection and deoxynivalenol contamination in winter wheat
T1  - Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice
VL  - 31
IS  - 1
SP  - 123
EP  - 131
DO  - 10.2298/BAH1501123K
ER  - 
@article{
author = "Krnjaja, Vesna and Tomić, Z. and Stanković, Slavica and Petrović, T. and Bijelić, Zorica and Mandić, Violeta and Obradović, Ana",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/606",
abstract = "In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat., U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fusarium infection and deoxynivalenol contamination in winter wheat, Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice",
volume = "31",
number = "1",
pages = "123-131",
doi = "10.2298/BAH1501123K"
}
Krnjaja, V., Tomić, Z., Stanković, S., Petrović, T., Bijelić, Z., Mandić, V.,& Obradović, A. (2015). Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 31(1), 123-131.
https://doi.org/10.2298/BAH1501123K
Krnjaja V, Tomić Z, Stanković S, Petrović T, Bijelić Z, Mandić V, Obradović A. Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice. Biotechnology in Animal Husbandry. 2015;31(1):123-131
Krnjaja Vesna, Tomić Z., Stanković Slavica, Petrović T., Bijelić Zorica, Mandić Violeta, Obradović Ana, "Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice" 31, no. 1 (2015):123-131,
https://doi.org/10.2298/BAH1501123K .
5

Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat

Krnjaja, Vesna; Mandić, Violeta; Lević, Jelena; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/602
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
VL  - 67
SP  - 251
EP  - 256
DO  - 10.1016/j.cropro.2014.11.001
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/602",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
volume = "67",
pages = "251-256",
doi = "10.1016/j.cropro.2014.11.001"
}
Krnjaja, V., Mandić, V., Lević, J., Stanković, S., Obradović, A., Petrović, T.,& Vasić, T. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat.
Crop ProtectionElsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001
Krnjaja V, Mandić V, Lević J, Stanković S, Obradović A, Petrović T, Vasić T. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. Crop Protection. 2015;67:251-256
Krnjaja Vesna, Mandić Violeta, Lević Jelena, Stanković Slavica, Obradović Ana, Petrović Tanja, Vasić Tanja, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 .
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