TY  - JOUR
AU  - Uzelac, Iva
AU  - Avramov, Miloš
AU  - Vukašinović, Elvira
AU  - Gošić-Dondo, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Blagojević, Duško
AU  - Popović, Željko D.
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/793
AB  - The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.
PB  - Berlin : Nature Research is part of Springer Nature
T2  - Scientific Reports
T1  - Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis Hbn
VL  - 10
IS  - 1
SP  - 9085
DO  - 10.1038/s41598-020-65926-w
ER  - 

@article{
author = "Uzelac, Iva and Avramov, Miloš and Vukašinović, Elvira and Gošić-Dondo, Snežana and Purać, Jelena and Kojić, Danijela and Blagojević, Duško and Popović, Željko D.",
year = "2020",
abstract = "The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.",
publisher = "Berlin : Nature Research is part of Springer Nature",
journal = "Scientific Reports",
title = "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis Hbn",
volume = "10",
number = "1",
pages = "9085",
doi = "10.1038/s41598-020-65926-w"
}

Uzelac, I., Avramov, M., Vukašinović, E., Gošić-Dondo, S., Purać, J., Kojić, D., Blagojević, D.,& Popović, Ž. D.. (2020). Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis Hbn. in Scientific Reports
Berlin : Nature Research is part of Springer Nature., 10(1), 9085.
https://doi.org/10.1038/s41598-020-65926-w

Uzelac I, Avramov M, Vukašinović E, Gošić-Dondo S, Purać J, Kojić D, Blagojević D, Popović ŽD. Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis Hbn. in Scientific Reports. 2020;10(1):9085.
doi:10.1038/s41598-020-65926-w .

Uzelac, Iva, Avramov, Miloš, Vukašinović, Elvira, Gošić-Dondo, Snežana, Purać, Jelena, Kojić, Danijela, Blagojević, Duško, Popović, Željko D., "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis Hbn" in Scientific Reports, 10, no. 1 (2020):9085,
https://doi.org/10.1038/s41598-020-65926-w . .