Ristić, Danijela

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  • Ristić, Danijela (35)

Author's Bibliography

Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit

Kravić, Natalija; Babić, Vojka; Vukadinović, Jelena; Ristić, Danijela; Dragičević, Vesna; Mladenović-Drinić, Snežana; Anđelković, Violeta

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Vukadinović, Jelena
AU  - Ristić, Danijela
AU  - Dragičević, Vesna
AU  - Mladenović-Drinić, Snežana
AU  - Anđelković, Violeta
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/828
AB  - Plants reconfigure their metabolic pathways to cope with water deficit. The aim of this study was to determine the status of the physiological parameters and the content of phenolic acids in the upper most ear leaf of maize inbred lines contrasting in drought tolerance in terms of improved plant productivity e.g., increased grain yield. The experiment was conducted under irrigation and rain-fed conditions. In drought-tolerant lines, the effect of water deficit was reflected through a chlorophyll and nitrogen balance index increase followed by a flavonols index decrease. The opposite trend was noticed in drought susceptible inbreds, with the exception of the anthocyanins index. Moreover, in comparison to irrigation treatment, opposite trends in the correlations between grain yield and physiological parameters found under water deficit conditions indicated the activation of different metabolic pathways in defense against water deficit stress. Concerning phenolic acid content, water deficit caused the reduction of protocatechuic, caffeic, and sinapic acid in all inbreds evaluated. However, the highly pronounced increase of ferulic and especially cinnamic acid content under water deficit conditions indicated possible crucial role of these secondary metabolites in preventing the harmful effects of water deficit stress, which, in turn, might be useful in maize breeding selection for drought tolerance
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biology
T1  - Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit
VL  - 10
IS  - 8
SP  - 694
DO  - 10.3390/biology10080694
ER  - 
@article{
author = "Kravić, Natalija and Babić, Vojka and Vukadinović, Jelena and Ristić, Danijela and Dragičević, Vesna and Mladenović-Drinić, Snežana and Anđelković, Violeta",
year = "2021",
abstract = "Plants reconfigure their metabolic pathways to cope with water deficit. The aim of this study was to determine the status of the physiological parameters and the content of phenolic acids in the upper most ear leaf of maize inbred lines contrasting in drought tolerance in terms of improved plant productivity e.g., increased grain yield. The experiment was conducted under irrigation and rain-fed conditions. In drought-tolerant lines, the effect of water deficit was reflected through a chlorophyll and nitrogen balance index increase followed by a flavonols index decrease. The opposite trend was noticed in drought susceptible inbreds, with the exception of the anthocyanins index. Moreover, in comparison to irrigation treatment, opposite trends in the correlations between grain yield and physiological parameters found under water deficit conditions indicated the activation of different metabolic pathways in defense against water deficit stress. Concerning phenolic acid content, water deficit caused the reduction of protocatechuic, caffeic, and sinapic acid in all inbreds evaluated. However, the highly pronounced increase of ferulic and especially cinnamic acid content under water deficit conditions indicated possible crucial role of these secondary metabolites in preventing the harmful effects of water deficit stress, which, in turn, might be useful in maize breeding selection for drought tolerance",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biology",
title = "Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit",
volume = "10",
number = "8",
pages = "694",
doi = "10.3390/biology10080694"
}
Kravić, N., Babić, V., Vukadinović, J., Ristić, D., Dragičević, V., Mladenović-Drinić, S.,& Anđelković, V.. (2021). Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit. in Biology
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 694.
https://doi.org/10.3390/biology10080694
Kravić N, Babić V, Vukadinović J, Ristić D, Dragičević V, Mladenović-Drinić S, Anđelković V. Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit. in Biology. 2021;10(8):694.
doi:10.3390/biology10080694 .
Kravić, Natalija, Babić, Vojka, Vukadinović, Jelena, Ristić, Danijela, Dragičević, Vesna, Mladenović-Drinić, Snežana, Anđelković, Violeta, "Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit" in Biology, 10, no. 8 (2021):694,
https://doi.org/10.3390/biology10080694 . .
2
2

Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation

Kostadinović, Marija; Janjić, Jovana; Ristić, Danijela; Đorđević-Melnik, Olivera; Božinović, Sofija; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2020)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Janjić, Jovana
AU  - Ristić, Danijela
AU  - Đorđević-Melnik, Olivera
AU  - Božinović, Sofija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/962
AB  - This paper is a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions using the marker assisted selection. When SSR marker specific to the crtRB1 gene has been tested on parental lines, distinct polymorphism was observed between the donor and recurrent parents. The same marker has also been validated in BC1 generation when heterozygous individuals were clearly distinguishable from the homozygous dominants. The results presented herein refer to two-level selection procedure in BC2 generation. First, BC2 plants were analyzed with crtRB1-specific molecular marker to separate heterozygotes from dominant homozygotes. Percentage of heterozygous plants was approximately 50%, which was in accordance with the expected Mendelian ratio of 1:1. Second, the selected heterozygotes were screened with 30 polymorphic SSR markers distributed throughout the maize genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The RPG values among three analyzed parental lines and their respective progenies ranged from 85-99%. For each line separately, RPG values were: 86-97% (RP1), 90-95% (RP2) and 85-99% (RP3). Plants with RPG above 95% were selfed to produce BC2F2 generation in which homozygous recessive individuals would be identified. Those genotypes will be subjected to biochemical and phenotypic evaluation to confirm their nutritional and agronomical superiority. Finally, these β-carotene enriched lines, as well as the resulting improved hybrids, can be used in the biofortification programs.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings
T1  - Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation
SP  - 37
EP  - 44
ER  - 
@conference{
author = "Kostadinović, Marija and Janjić, Jovana and Ristić, Danijela and Đorđević-Melnik, Olivera and Božinović, Sofija and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2020",
abstract = "This paper is a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions using the marker assisted selection. When SSR marker specific to the crtRB1 gene has been tested on parental lines, distinct polymorphism was observed between the donor and recurrent parents. The same marker has also been validated in BC1 generation when heterozygous individuals were clearly distinguishable from the homozygous dominants. The results presented herein refer to two-level selection procedure in BC2 generation. First, BC2 plants were analyzed with crtRB1-specific molecular marker to separate heterozygotes from dominant homozygotes. Percentage of heterozygous plants was approximately 50%, which was in accordance with the expected Mendelian ratio of 1:1. Second, the selected heterozygotes were screened with 30 polymorphic SSR markers distributed throughout the maize genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The RPG values among three analyzed parental lines and their respective progenies ranged from 85-99%. For each line separately, RPG values were: 86-97% (RP1), 90-95% (RP2) and 85-99% (RP3). Plants with RPG above 95% were selfed to produce BC2F2 generation in which homozygous recessive individuals would be identified. Those genotypes will be subjected to biochemical and phenotypic evaluation to confirm their nutritional and agronomical superiority. Finally, these β-carotene enriched lines, as well as the resulting improved hybrids, can be used in the biofortification programs.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings",
title = "Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation",
pages = "37-44"
}
Kostadinović, M., Janjić, J., Ristić, D., Đorđević-Melnik, O., Božinović, S., Ignjatović-Micić, D.,& Vančetović, J.. (2020). Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation. in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 37-44.
Kostadinović M, Janjić J, Ristić D, Đorđević-Melnik O, Božinović S, Ignjatović-Micić D, Vančetović J. Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation. in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings. 2020;:37-44..
Kostadinović, Marija, Janjić, Jovana, Ristić, Danijela, Đorđević-Melnik, Olivera, Božinović, Sofija, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation" in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings (2020):37-44.

Morphological and physiological response of maize seedlings to chilling stress

Nikolić, Ana; Kravić, Natalija; Ristić, Danijela; Anđelković, Violeta; Marković, Ksenija; Vančetović, Jelena; Ignjatović-Micić, Dragana

(Beograd : Društavo genetičara Srbije, 2020)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kravić, Natalija
AU  - Ristić, Danijela
AU  - Anđelković, Violeta
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/814
AB  - Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing.
AB  - Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve
PB  - Beograd : Društavo genetičara Srbije
T2  - Genetika
T1  - Morphological and physiological response of maize seedlings to chilling stress
VL  - 52
IS  - 2
SP  - 689
EP  - 698
DO  - 10.2298/GENSR2002689N
ER  - 
@article{
author = "Nikolić, Ana and Kravić, Natalija and Ristić, Danijela and Anđelković, Violeta and Marković, Ksenija and Vančetović, Jelena and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing., Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve",
publisher = "Beograd : Društavo genetičara Srbije",
journal = "Genetika",
title = "Morphological and physiological response of maize seedlings to chilling stress",
volume = "52",
number = "2",
pages = "689-698",
doi = "10.2298/GENSR2002689N"
}
Nikolić, A., Kravić, N., Ristić, D., Anđelković, V., Marković, K., Vančetović, J.,& Ignjatović-Micić, D.. (2020). Morphological and physiological response of maize seedlings to chilling stress. in Genetika
Beograd : Društavo genetičara Srbije., 52(2), 689-698.
https://doi.org/10.2298/GENSR2002689N
Nikolić A, Kravić N, Ristić D, Anđelković V, Marković K, Vančetović J, Ignjatović-Micić D. Morphological and physiological response of maize seedlings to chilling stress. in Genetika. 2020;52(2):689-698.
doi:10.2298/GENSR2002689N .
Nikolić, Ana, Kravić, Natalija, Ristić, Danijela, Anđelković, Violeta, Marković, Ksenija, Vančetović, Jelena, Ignjatović-Micić, Dragana, "Morphological and physiological response of maize seedlings to chilling stress" in Genetika, 52, no. 2 (2020):689-698,
https://doi.org/10.2298/GENSR2002689N . .
2
2

Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Ristić, Danijela; Obradović, Ana; Đorđević-Melnik, Olivera; Kovinčić, Anika

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2019)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Obradović, Ana
AU  - Đorđević-Melnik, Olivera
AU  - Kovinčić, Anika
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/964
AB  - Marker assisted selection (MAS) significantly increases efficiency of conventional breeding. Molecular markers are utilized as selection markers for target genes (foreground selection) and also for identification of the genotypes (progenies) with the highest proportion of recurrent parent’s genome (background selection). Maize Research Institute "Zemun Polje" has a breeding program with the aim to create lines with incorporated incompatibility dominant gene Gametophytic Factor 1-S (Gа1-S), using the integrated conventional and molecular breeding approach. Ga1-S is the most described gene belonging to the group of genes specific to the pollen development, germination and pollen tube growth. The Ga1-S system is the most commonly used to prevent the pollination of sweetcorn, popcorn and white kerneld hybrids by standard maize. The objectives of this study were identification of gene-specific molecular marker for foreground selection, as well as the set of SSR markers polymorphic between parental lines to be used in background selection. Genetic variability between two donor and three recurrent parental inbred lines was analyzed with 42 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 83, average being 2.77. The genetic similarity values calculated on Dice coefficient ranged from 0.47 to 0.71. Among 12 gene-specific markers tested on parental lines, two showed distinct polymorphism for Ga1-S. These markers will be used as foreground selection markers for the incorporation of Gа1-S into our inbred lines which will be used for the creation of white kernel hybrids.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings
T1  - Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines
SP  - 91
EP  - 95
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Obradović, Ana and Đorđević-Melnik, Olivera and Kovinčić, Anika",
year = "2019",
abstract = "Marker assisted selection (MAS) significantly increases efficiency of conventional breeding. Molecular markers are utilized as selection markers for target genes (foreground selection) and also for identification of the genotypes (progenies) with the highest proportion of recurrent parent’s genome (background selection). Maize Research Institute "Zemun Polje" has a breeding program with the aim to create lines with incorporated incompatibility dominant gene Gametophytic Factor 1-S (Gа1-S), using the integrated conventional and molecular breeding approach. Ga1-S is the most described gene belonging to the group of genes specific to the pollen development, germination and pollen tube growth. The Ga1-S system is the most commonly used to prevent the pollination of sweetcorn, popcorn and white kerneld hybrids by standard maize. The objectives of this study were identification of gene-specific molecular marker for foreground selection, as well as the set of SSR markers polymorphic between parental lines to be used in background selection. Genetic variability between two donor and three recurrent parental inbred lines was analyzed with 42 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 83, average being 2.77. The genetic similarity values calculated on Dice coefficient ranged from 0.47 to 0.71. Among 12 gene-specific markers tested on parental lines, two showed distinct polymorphism for Ga1-S. These markers will be used as foreground selection markers for the incorporation of Gа1-S into our inbred lines which will be used for the creation of white kernel hybrids.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings",
title = "Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines",
pages = "91-95"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D., Obradović, A., Đorđević-Melnik, O.,& Kovinčić, A.. (2019). Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines. in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 91-95.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Obradović A, Đorđević-Melnik O, Kovinčić A. Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines. in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings. 2019;:91-95..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Obradović, Ana, Đorđević-Melnik, Olivera, Kovinčić, Anika, "Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines" in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings (2019):91-95.

The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version

Kostadinović, Marija; Ristić, Danijela; Kovinčić, Anika; Simić, Marijana; Perić, Vesna; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Belgrade : Serbian Genetic Society, 2019)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Kovinčić, Anika
AU  - Simić, Marijana
AU  - Perić, Vesna
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/963
AB  - Quality Protein Maize (QPM) is nutritionally and agronomically improved maize. In order to shorten the period required for development of QPM hybrids through the conventional method of backcrossing, marker assisted selection (MAS) is being used. After successful conversion of one commercial maize inbred line to its QPM counterpart for growing in temperate climate, this breeding program was continued at Maize Research Institute (MRI) with larger number of maize lines. Four commercial MRI inbreds, chosen for marker assisted introgression of the quality protein trait, and their BC2 progenies were subjected to two-level selection procedure. First, BC2 plants were analyzed with opaque2 (o2) specific molecular markers to identify heterozygotes. Second, the selected heterozygotes were screened with SSR markers distributed throughout the genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The specific markers identified 100 out of 192 plants (52%) as heterozygous. Genetic similarity values between parental lines and their BC2 heterozygous progenies were in the range from 0.77 to 0.99 (77-99% RPG). The highest proportion of RPG was found in L1 (93-99%) and the lowest in L3 progenies (77-89%). Average values for the RPG content ranged from 83.9 to 95.8%. Progenies with RPG above 95% were selfed to produce BC2F2 plants which will be subjected to foreground selection. This time selection will be focused on homozygous recessive individuals, given that the presence of opaque2 gene in the homozygous recessive state is the aim of the QPM selection. Finally, those o2o2 genotypes will be screened for biochemical and phenotypic traits to confirm their nutritional and agronomical superiority.
PB  - Belgrade : Serbian Genetic Society
C3  - 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings
T1  - The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version
SP  - 23
EP  - 30
ER  - 
@conference{
author = "Kostadinović, Marija and Ristić, Danijela and Kovinčić, Anika and Simić, Marijana and Perić, Vesna and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Quality Protein Maize (QPM) is nutritionally and agronomically improved maize. In order to shorten the period required for development of QPM hybrids through the conventional method of backcrossing, marker assisted selection (MAS) is being used. After successful conversion of one commercial maize inbred line to its QPM counterpart for growing in temperate climate, this breeding program was continued at Maize Research Institute (MRI) with larger number of maize lines. Four commercial MRI inbreds, chosen for marker assisted introgression of the quality protein trait, and their BC2 progenies were subjected to two-level selection procedure. First, BC2 plants were analyzed with opaque2 (o2) specific molecular markers to identify heterozygotes. Second, the selected heterozygotes were screened with SSR markers distributed throughout the genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The specific markers identified 100 out of 192 plants (52%) as heterozygous. Genetic similarity values between parental lines and their BC2 heterozygous progenies were in the range from 0.77 to 0.99 (77-99% RPG). The highest proportion of RPG was found in L1 (93-99%) and the lowest in L3 progenies (77-89%). Average values for the RPG content ranged from 83.9 to 95.8%. Progenies with RPG above 95% were selfed to produce BC2F2 plants which will be subjected to foreground selection. This time selection will be focused on homozygous recessive individuals, given that the presence of opaque2 gene in the homozygous recessive state is the aim of the QPM selection. Finally, those o2o2 genotypes will be screened for biochemical and phenotypic traits to confirm their nutritional and agronomical superiority.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings",
title = "The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version",
pages = "23-30"
}
Kostadinović, M., Ristić, D., Kovinčić, A., Simić, M., Perić, V., Ignjatović-Micić, D.,& Vančetović, J.. (2019). The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version. in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings
Belgrade : Serbian Genetic Society., 23-30.
Kostadinović M, Ristić D, Kovinčić A, Simić M, Perić V, Ignjatović-Micić D, Vančetović J. The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version. in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings. 2019;:23-30..
Kostadinović, Marija, Ristić, Danijela, Kovinčić, Anika, Simić, Marijana, Perić, Vesna, Ignjatović-Micić, Dragana, Vančetović, Jelena, "The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version" in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings (2019):23-30.

Marker assisted backcross breeding in Maize Research Institute Zemun Polje

Kostadinović, Marija; Nikolić, Ana; Ristić, Danijela; Božinović, Sofija; Đorđević-Melnik, Olivera; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2019)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/743
AB  - Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije.
AB  - The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Marker assisted backcross breeding in Maize Research Institute Zemun Polje
T1  - Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje
VL  - 25
IS  - 1
SP  - 41
EP  - 47
DO  - 10.5937/SelSem1901041K
UR  - conv_2003
ER  - 
@article{
author = "Kostadinović, Marija and Nikolić, Ana and Ristić, Danijela and Božinović, Sofija and Đorđević-Melnik, Olivera and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije., The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Marker assisted backcross breeding in Maize Research Institute Zemun Polje, Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje",
volume = "25",
number = "1",
pages = "41-47",
doi = "10.5937/SelSem1901041K",
url = "conv_2003"
}
Kostadinović, M., Nikolić, A., Ristić, D., Božinović, S., Đorđević-Melnik, O., Ignjatović-Micić, D.,& Vančetović, J.. (2019). Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 25(1), 41-47.
https://doi.org/10.5937/SelSem1901041K
conv_2003
Kostadinović M, Nikolić A, Ristić D, Božinović S, Đorđević-Melnik O, Ignjatović-Micić D, Vančetović J. Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo. 2019;25(1):41-47.
doi:10.5937/SelSem1901041K
conv_2003 .
Kostadinović, Marija, Nikolić, Ana, Ristić, Danijela, Božinović, Sofija, Đorđević-Melnik, Olivera, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted backcross breeding in Maize Research Institute Zemun Polje" in Selekcija i semenarstvo, 25, no. 1 (2019):41-47,
https://doi.org/10.5937/SelSem1901041K .,
conv_2003 .
3

Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain

Popović, Vladan; Lučić, Aleksandar; Rakonjac, Ljubinko; Milovanović, Jelena; Mladenović-Drinić, Snežana; Ristić, Danijela

(Beograd : Društvo genetičara Srbije, 2019)

TY  - JOUR
AU  - Popović, Vladan
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Milovanović, Jelena
AU  - Mladenović-Drinić, Snežana
AU  - Ristić, Danijela
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/799
AB  - The process of plant breeding and conservation of gene pool among other things depends on the knowledge of the level of genetic differentiation. The aim of research in this paper was to determine the genetic differentiation of silver fir (Abies albaMill.) populations of regular  type  and  atypical  genotypes  with  pyramidal  crown  that  can  be  found  on  Javor mountain, at  the  site Ogorijevac. The  genetic  differentiation of silver fir  was determined using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave 29 alleles, while  the  average  number  of  alleles  was  3.2.  The  primer  NFH15  gave  the  smallest number of alleles (two), while the primer SF78 gave the greatest number of alleles (five). Dice  coefficient  of  the  genetic  similarity  was  used  to  obtain  a  dendrogram  by  UPMGA analysis  using  NTSYSpc  statistical  program.  The  genetic  similarity  recorded  among  the individuals P1 and P2 was the largest (0.89), while the populations VI and individual P2 showed the lowest similarity (0.61).Based on the cluster analysis it canbe concluded that the  studied populations and genotypes of silver  fir  with different types of crown are  clearly differentiated. The  basic insight  into  the  level  of  the  genetic  diversity  of  the  natural  populations  of  silver  fir  with the  various types of crown has been provided using selected SSR markers. The obtained results can be used for creating further strategy for the conservation of the available gene pool and the regeneration of silver fir forests in Serbia.
AB  - Proces oplemenjivanja biljaka i konzervacije genofonda između ostalog, zavisi i od poznavanja stepena  genetičke  diferencijacije.  Cilj  istraživanja  u  ovom  radu  bio  je  da  se  utvrdi  genetička diferencijacija   populacija   jele   (Abies   alba Mill.)normalanog  tipa  i  atipičnih  genotipova piramidalanog tipa krošnje, koje se javljaju na planini Javor, na lokalitetu Ogorijevac. Genetička sličnost odnosno različitost jele određena je upotrebom SSR (Simple Sequence Repeat) markera. Devet SSR pari prajmera dalo je ukupno 29 alela, dok je prosečan broj alela bio 3,2. Prajmer NFH15 dao je najmanji broj alela (dva), dok je prajmer SF78 bio sa najvećim brojem alela (pet). Najviša vrednost koeficijenta genetičke sličnosti utvrđena je između genotipova P1 i P2 (0,89), dok je najniža vrednost koeficijenta genetičke sličnosti utvrđena između populacije VI i genotipa P2 (0,61).Koeficijenti  genetičke  sličnosti  po  Dice,  upotrebljeni  su  za  dobijanje  dendrograma  pomoću UPMGA  analize,  koristeći  NTSYSpc  statistički  program.  Na  osnovu  klaster  analize  može  se zaključiti  da  su  istraživane  populacije  i  genotipovi  jele  sa  različitim  tipom  krošnje  jasno izdiferencirani.  Upotrebom  izabranih  SSR  markera  dat  je  osnovni  uvid  u  nivo  genetičke raznovrsnosti prirodnih populacija jele različitog tipa krošnje. Dobijeni rezultati mogu poslužiti u budućoj strategiji na konzervaciji raspoloživog genofonda i obnavljanju jelovih šuma u Srbiji.
PB  - Beograd : Društvo genetičara Srbije
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain
T1  - Procena genetičke diferencijacije jele (Abies alba Mill.) sa Javora pomoću SSR markera
VL  - 51
IS  - 3
SP  - 1103
EP  - 1112
DO  - 10.2298/GENSR1903103P
ER  - 
@article{
author = "Popović, Vladan and Lučić, Aleksandar and Rakonjac, Ljubinko and Milovanović, Jelena and Mladenović-Drinić, Snežana and Ristić, Danijela",
year = "2019",
abstract = "The process of plant breeding and conservation of gene pool among other things depends on the knowledge of the level of genetic differentiation. The aim of research in this paper was to determine the genetic differentiation of silver fir (Abies albaMill.) populations of regular  type  and  atypical  genotypes  with  pyramidal  crown  that  can  be  found  on  Javor mountain, at  the  site Ogorijevac. The  genetic  differentiation of silver fir  was determined using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave 29 alleles, while  the  average  number  of  alleles  was  3.2.  The  primer  NFH15  gave  the  smallest number of alleles (two), while the primer SF78 gave the greatest number of alleles (five). Dice  coefficient  of  the  genetic  similarity  was  used  to  obtain  a  dendrogram  by  UPMGA analysis  using  NTSYSpc  statistical  program.  The  genetic  similarity  recorded  among  the individuals P1 and P2 was the largest (0.89), while the populations VI and individual P2 showed the lowest similarity (0.61).Based on the cluster analysis it canbe concluded that the  studied populations and genotypes of silver  fir  with different types of crown are  clearly differentiated. The  basic insight  into  the  level  of  the  genetic  diversity  of  the  natural  populations  of  silver  fir  with the  various types of crown has been provided using selected SSR markers. The obtained results can be used for creating further strategy for the conservation of the available gene pool and the regeneration of silver fir forests in Serbia., Proces oplemenjivanja biljaka i konzervacije genofonda između ostalog, zavisi i od poznavanja stepena  genetičke  diferencijacije.  Cilj  istraživanja  u  ovom  radu  bio  je  da  se  utvrdi  genetička diferencijacija   populacija   jele   (Abies   alba Mill.)normalanog  tipa  i  atipičnih  genotipova piramidalanog tipa krošnje, koje se javljaju na planini Javor, na lokalitetu Ogorijevac. Genetička sličnost odnosno različitost jele određena je upotrebom SSR (Simple Sequence Repeat) markera. Devet SSR pari prajmera dalo je ukupno 29 alela, dok je prosečan broj alela bio 3,2. Prajmer NFH15 dao je najmanji broj alela (dva), dok je prajmer SF78 bio sa najvećim brojem alela (pet). Najviša vrednost koeficijenta genetičke sličnosti utvrđena je između genotipova P1 i P2 (0,89), dok je najniža vrednost koeficijenta genetičke sličnosti utvrđena između populacije VI i genotipa P2 (0,61).Koeficijenti  genetičke  sličnosti  po  Dice,  upotrebljeni  su  za  dobijanje  dendrograma  pomoću UPMGA  analize,  koristeći  NTSYSpc  statistički  program.  Na  osnovu  klaster  analize  može  se zaključiti  da  su  istraživane  populacije  i  genotipovi  jele  sa  različitim  tipom  krošnje  jasno izdiferencirani.  Upotrebom  izabranih  SSR  markera  dat  je  osnovni  uvid  u  nivo  genetičke raznovrsnosti prirodnih populacija jele različitog tipa krošnje. Dobijeni rezultati mogu poslužiti u budućoj strategiji na konzervaciji raspoloživog genofonda i obnavljanju jelovih šuma u Srbiji.",
publisher = "Beograd : Društvo genetičara Srbije, Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain, Procena genetičke diferencijacije jele (Abies alba Mill.) sa Javora pomoću SSR markera",
volume = "51",
number = "3",
pages = "1103-1112",
doi = "10.2298/GENSR1903103P"
}
Popović, V., Lučić, A., Rakonjac, L., Milovanović, J., Mladenović-Drinić, S.,& Ristić, D.. (2019). Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain. in Genetika
Beograd : Društvo genetičara Srbije., 51(3), 1103-1112.
https://doi.org/10.2298/GENSR1903103P
Popović V, Lučić A, Rakonjac L, Milovanović J, Mladenović-Drinić S, Ristić D. Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain. in Genetika. 2019;51(3):1103-1112.
doi:10.2298/GENSR1903103P .
Popović, Vladan, Lučić, Aleksandar, Rakonjac, Ljubinko, Milovanović, Jelena, Mladenović-Drinić, Snežana, Ristić, Danijela, "Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain" in Genetika, 51, no. 3 (2019):1103-1112,
https://doi.org/10.2298/GENSR1903103P . .
1
1
1

Parental polymorphism analysis in marker assisted selection for β-carotene rich maize

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Ristić, Danijela; Obradović, Ana; Stevanović, Milan; Mladenović-Drinić, Snežana

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2018)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Obradović, Ana
AU  - Stevanović, Milan
AU  - Mladenović-Drinić, Snežana
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/960
AB  - Marker assisted selection (MAS) is widely implemented into modern grain breeding programs. Molecular markers are used in foreground selection to control the target gene, as well as in background selection to accelerate the reconstruction of the recurrent parent genotype. The best results have been achieved with the qualitative traits, regulated by the action of a single or several genes and clearly phenotypically defined. One successful example of MAS is the improvement of β-carotene content using crtRB1 specific molecular marker. Maize Research Institute "Zemun Polje" has a breeding program aimed at conversion of standard maize to β-carotene rich genotypes adapted to temperate regions. The objectives of this study were to test the utility of gene-specific SSR marker in foreground selection and to identify polymorphic markers between parental lines to be used in background selection. Genetic variability between two standard and three high β-carotene parental inbred lines was analyzed with 40 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 77, average being 2.57. The genetic similarity values calculated on Dice coefficient ranged from 0.49 to 0.66. Parental polymorphism for crtRB1 showed a 543 bp fragment in donor lines, whereas a distinct 296 bp amplicon and a faint 1221 bp amplicon were generated in the recurrent parents. This marker will be used as foreground selection marker for the crtRB1 gene in the conversion of standard maize to β-carotene enriched lines for growing in temperate regions.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings
T1  - Parental polymorphism analysis in marker assisted selection for β-carotene rich maize
SP  - 333
EP  - 338
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Obradović, Ana and Stevanović, Milan and Mladenović-Drinić, Snežana",
year = "2018",
abstract = "Marker assisted selection (MAS) is widely implemented into modern grain breeding programs. Molecular markers are used in foreground selection to control the target gene, as well as in background selection to accelerate the reconstruction of the recurrent parent genotype. The best results have been achieved with the qualitative traits, regulated by the action of a single or several genes and clearly phenotypically defined. One successful example of MAS is the improvement of β-carotene content using crtRB1 specific molecular marker. Maize Research Institute "Zemun Polje" has a breeding program aimed at conversion of standard maize to β-carotene rich genotypes adapted to temperate regions. The objectives of this study were to test the utility of gene-specific SSR marker in foreground selection and to identify polymorphic markers between parental lines to be used in background selection. Genetic variability between two standard and three high β-carotene parental inbred lines was analyzed with 40 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 77, average being 2.57. The genetic similarity values calculated on Dice coefficient ranged from 0.49 to 0.66. Parental polymorphism for crtRB1 showed a 543 bp fragment in donor lines, whereas a distinct 296 bp amplicon and a faint 1221 bp amplicon were generated in the recurrent parents. This marker will be used as foreground selection marker for the crtRB1 gene in the conversion of standard maize to β-carotene enriched lines for growing in temperate regions.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings",
title = "Parental polymorphism analysis in marker assisted selection for β-carotene rich maize",
pages = "333-338"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D., Obradović, A., Stevanović, M.,& Mladenović-Drinić, S.. (2018). Parental polymorphism analysis in marker assisted selection for β-carotene rich maize. in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 333-338.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Obradović A, Stevanović M, Mladenović-Drinić S. Parental polymorphism analysis in marker assisted selection for β-carotene rich maize. in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings. 2018;:333-338..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Obradović, Ana, Stevanović, Milan, Mladenović-Drinić, Snežana, "Parental polymorphism analysis in marker assisted selection for β-carotene rich maize" in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings (2018):333-338.

Uticaj starosti semena na kvalitet različitih sorata lucerke

Štrbanović, Ratibor; Poštić, Dobrivoj; Ristić, Danijela; Marković, Jordan; Jovanović, Snežana; Tabaković, Marijenka; Stanisavljević, Rade

(Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi, 2018)

TY  - JOUR
AU  - Štrbanović, Ratibor
AU  - Poštić, Dobrivoj
AU  - Ristić, Danijela
AU  - Marković, Jordan
AU  - Jovanović, Snežana
AU  - Tabaković, Marijenka
AU  - Stanisavljević, Rade
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/946
AB  - Plava lucerka (Medicago sativa L.) je zbog svih svojih osobina najvažnija krmna biljka u 
našoj zemlji. Veliki značaj lucerke se ogleda u proizvodnji kvalitetne kabaste hrane, ali je i 
seme lucerke vredna i kurentna roba na domaćem i inostranom tržištu. Areal gajenja lucerke 
je na svim kontinentima u više od 80 zemalja, od umereno hladnog do tropskog pojasa. Široka 
geografska rasprostranjenost lucerke uslovljena je njenom velikom adaptabilnošću na različite 
klimatske i zemljišne uslove. Zasnivanje useva krmnih biljaka vrši se direktnom setvom
semena. Obzirom na te okolnosti, brza i uniformna pojava snaznih klijanaca lucerke je veoma 
značajna kako bi se osigurala visoka uniformnost klijanaca, što utiče na početnu fazu razvoja 
useva, a kao rezultat svega je visok i stabilan prinos. Sve ovo ukazuje na važnost izbora 
semena dobrog kvaliteta.U ovom radu je ispitivan uticaj starosti semena na kvalitet tri 
različite sorte lucerke (Kruševačka 28, NS-Banat i Čačanka-10). Seme ispitivanih sorata 
lucerke čuvano je pet godina u papirnim kesama u kontrolisanim uslovima temperature i 
relativne vlažnosti vazduha. Zatim je ispitivana klijavost semena različitih sorata lucerke 
starosti pet godina u klijalistu na temperaturi od 20o
C u mraku, deset dana (prema Pravilniku 
o ispitivanju kvaliteta semena poljoprivrednog bilja 47/87). Dobijeni rezultati ukazuju da je 
starost semena značajno uticala na ukupnu klijavost, nenormalne klijance, tvrdo i mrtvo seme. 
Najveća klijavost je ostvarena iz semena starog dve godine, pri tome uticaj sorte je takodje 
statistički značajno delovao na ukupnu klijavost, kao i interakcija starost semena x sorta 
(P≤0,05).
PB  - Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - 30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda
T1  - Uticaj starosti semena na kvalitet različitih sorata lucerke
SP  - 115
EP  - 115
ER  - 
@article{
author = "Štrbanović, Ratibor and Poštić, Dobrivoj and Ristić, Danijela and Marković, Jordan and Jovanović, Snežana and Tabaković, Marijenka and Stanisavljević, Rade",
year = "2018",
abstract = "Plava lucerka (Medicago sativa L.) je zbog svih svojih osobina najvažnija krmna biljka u 
našoj zemlji. Veliki značaj lucerke se ogleda u proizvodnji kvalitetne kabaste hrane, ali je i 
seme lucerke vredna i kurentna roba na domaćem i inostranom tržištu. Areal gajenja lucerke 
je na svim kontinentima u više od 80 zemalja, od umereno hladnog do tropskog pojasa. Široka 
geografska rasprostranjenost lucerke uslovljena je njenom velikom adaptabilnošću na različite 
klimatske i zemljišne uslove. Zasnivanje useva krmnih biljaka vrši se direktnom setvom
semena. Obzirom na te okolnosti, brza i uniformna pojava snaznih klijanaca lucerke je veoma 
značajna kako bi se osigurala visoka uniformnost klijanaca, što utiče na početnu fazu razvoja 
useva, a kao rezultat svega je visok i stabilan prinos. Sve ovo ukazuje na važnost izbora 
semena dobrog kvaliteta.U ovom radu je ispitivan uticaj starosti semena na kvalitet tri 
različite sorte lucerke (Kruševačka 28, NS-Banat i Čačanka-10). Seme ispitivanih sorata 
lucerke čuvano je pet godina u papirnim kesama u kontrolisanim uslovima temperature i 
relativne vlažnosti vazduha. Zatim je ispitivana klijavost semena različitih sorata lucerke 
starosti pet godina u klijalistu na temperaturi od 20o
C u mraku, deset dana (prema Pravilniku 
o ispitivanju kvaliteta semena poljoprivrednog bilja 47/87). Dobijeni rezultati ukazuju da je 
starost semena značajno uticala na ukupnu klijavost, nenormalne klijance, tvrdo i mrtvo seme. 
Najveća klijavost je ostvarena iz semena starog dve godine, pri tome uticaj sorte je takodje 
statistički značajno delovao na ukupnu klijavost, kao i interakcija starost semena x sorta 
(P≤0,05).",
publisher = "Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda",
title = "Uticaj starosti semena na kvalitet različitih sorata lucerke",
pages = "115-115"
}
Štrbanović, R., Poštić, D., Ristić, D., Marković, J., Jovanović, S., Tabaković, M.,& Stanisavljević, R.. (2018). Uticaj starosti semena na kvalitet različitih sorata lucerke. in 30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda
Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi., 115-115.
Štrbanović R, Poštić D, Ristić D, Marković J, Jovanović S, Tabaković M, Stanisavljević R. Uticaj starosti semena na kvalitet različitih sorata lucerke. in 30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda. 2018;:115-115..
Štrbanović, Ratibor, Poštić, Dobrivoj, Ristić, Danijela, Marković, Jordan, Jovanović, Snežana, Tabaković, Marijenka, Stanisavljević, Rade, "Uticaj starosti semena na kvalitet različitih sorata lucerke" in 30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda (2018):115-115.

Determination of free phenolic acids from leaves within different colored maize

Mesarović, Jelena; Dragičević, Vesna; Mladenović-Drinić, Snežana; Ristić, Danijela; Kravić, Natalija

(Srpsko hemijsko društvo, Beograd, 2017)

TY  - JOUR
AU  - Mesarović, Jelena
AU  - Dragičević, Vesna
AU  - Mladenović-Drinić, Snežana
AU  - Ristić, Danijela
AU  - Kravić, Natalija
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/678
AB  - Along with other plant parts, maize leaves are widely used for making fermented food for cattle, known as silage. Since there have only been a few reports on studies concerning the extraction and determination of phenolic acids from maize leaves, the main goal of this investigation was to evaluate the content of free phenolic acids in the leaves of fifteen different maize inbred lines. Reverse-phase, high performance liquid chromatography (RP-HPLC), with a photodiode array detector (DAD), was performed. Under the optimized chromatographic conditions, referring to short time of sample preparation, small quantities of solvent and direct injection of the extract into HPLC, phenolic acids (i.e., gallic, protocatechuic, caffeic, p-coumaric and ferulic acid) were successfully separated in less than 25 min, indicating that the method could be applied for routine analysis. The efficiency and validation of the method was evaluated by measuring the rate parameters: linearity, limit of detection and quantification, accuracy and precision. The obtained results showed that the most abundant free phenolic acid was p-coumaric acid (23.57 mu g g(-1) dry weight), followed by ferulic and caffeic acids (21.27 and 20.78 mu g g(-1) dry weight, respectively). Principal Component Analysis (PCA) revealed the existence of a link.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Determination of free phenolic acids from leaves within different colored maize
VL  - 82
IS  - 1
SP  - 63
EP  - 72
DO  - 10.2298/JSC160512104M
UR  - conv_949
ER  - 
@article{
author = "Mesarović, Jelena and Dragičević, Vesna and Mladenović-Drinić, Snežana and Ristić, Danijela and Kravić, Natalija",
year = "2017",
abstract = "Along with other plant parts, maize leaves are widely used for making fermented food for cattle, known as silage. Since there have only been a few reports on studies concerning the extraction and determination of phenolic acids from maize leaves, the main goal of this investigation was to evaluate the content of free phenolic acids in the leaves of fifteen different maize inbred lines. Reverse-phase, high performance liquid chromatography (RP-HPLC), with a photodiode array detector (DAD), was performed. Under the optimized chromatographic conditions, referring to short time of sample preparation, small quantities of solvent and direct injection of the extract into HPLC, phenolic acids (i.e., gallic, protocatechuic, caffeic, p-coumaric and ferulic acid) were successfully separated in less than 25 min, indicating that the method could be applied for routine analysis. The efficiency and validation of the method was evaluated by measuring the rate parameters: linearity, limit of detection and quantification, accuracy and precision. The obtained results showed that the most abundant free phenolic acid was p-coumaric acid (23.57 mu g g(-1) dry weight), followed by ferulic and caffeic acids (21.27 and 20.78 mu g g(-1) dry weight, respectively). Principal Component Analysis (PCA) revealed the existence of a link.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Determination of free phenolic acids from leaves within different colored maize",
volume = "82",
number = "1",
pages = "63-72",
doi = "10.2298/JSC160512104M",
url = "conv_949"
}
Mesarović, J., Dragičević, V., Mladenović-Drinić, S., Ristić, D.,& Kravić, N.. (2017). Determination of free phenolic acids from leaves within different colored maize. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 82(1), 63-72.
https://doi.org/10.2298/JSC160512104M
conv_949
Mesarović J, Dragičević V, Mladenović-Drinić S, Ristić D, Kravić N. Determination of free phenolic acids from leaves within different colored maize. in Journal of the Serbian Chemical Society. 2017;82(1):63-72.
doi:10.2298/JSC160512104M
conv_949 .
Mesarović, Jelena, Dragičević, Vesna, Mladenović-Drinić, Snežana, Ristić, Danijela, Kravić, Natalija, "Determination of free phenolic acids from leaves within different colored maize" in Journal of the Serbian Chemical Society, 82, no. 1 (2017):63-72,
https://doi.org/10.2298/JSC160512104M .,
conv_949 .
2
1
1

Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers

Popović, Vladan; Lučić, Aleksandar; Rakonjac, Ljubinko; Mladenović-Drinić, Snežana; Ristić, Danijela; Cvjetković, Branislav

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Popović, Vladan
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Mladenović-Drinić, Snežana
AU  - Ristić, Danijela
AU  - Cvjetković, Branislav
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/659
AB  - The paper presents the results of the analysis of genetic variability of eight populations of silver fir (Abies alba Mill.) in Serbia obtained using SSR markers. The genomic DNA was isolated from tissue of needles of all eight populations. Due to the costly and lengthy process a small number of the SSR markers for Abies alba have been developed, so in this study were used the microsatellite markers of related species. The obtained results indicate a low level of the genetic variability between natural populations of silver fir. The total number of alleles detected with nine SSR markers in eight studied populations of silver fir is 28. The range of alleles varies from two for NFF15 to six for SF78 with an average of 3.1 alleles per locus. The mean value of genetic similarity between populations is 0.92. The smallest genetic similarity between pairs of populations is 0.82 (Dubocica Bare and Stara Planina; Dubocica Bare and Tara) and the greatest genetic similarity is 1 (Zlatar and Stara Planina, Zlatar and Tara, Stara Planina and Tara). A basic insight into the level of genetic diversity of natural populations of silver fir in Serbia, which are located in a relatively small area, has been given using a set of SSR markers. The obtained results can be used in the future strategy for the management and regeneration of silver fir forests.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers
VL  - 49
IS  - 3
SP  - 979
EP  - 988
DO  - 10.2298/GENSR1703979P
UR  - conv_970
ER  - 
@article{
author = "Popović, Vladan and Lučić, Aleksandar and Rakonjac, Ljubinko and Mladenović-Drinić, Snežana and Ristić, Danijela and Cvjetković, Branislav",
year = "2017",
abstract = "The paper presents the results of the analysis of genetic variability of eight populations of silver fir (Abies alba Mill.) in Serbia obtained using SSR markers. The genomic DNA was isolated from tissue of needles of all eight populations. Due to the costly and lengthy process a small number of the SSR markers for Abies alba have been developed, so in this study were used the microsatellite markers of related species. The obtained results indicate a low level of the genetic variability between natural populations of silver fir. The total number of alleles detected with nine SSR markers in eight studied populations of silver fir is 28. The range of alleles varies from two for NFF15 to six for SF78 with an average of 3.1 alleles per locus. The mean value of genetic similarity between populations is 0.92. The smallest genetic similarity between pairs of populations is 0.82 (Dubocica Bare and Stara Planina; Dubocica Bare and Tara) and the greatest genetic similarity is 1 (Zlatar and Stara Planina, Zlatar and Tara, Stara Planina and Tara). A basic insight into the level of genetic diversity of natural populations of silver fir in Serbia, which are located in a relatively small area, has been given using a set of SSR markers. The obtained results can be used in the future strategy for the management and regeneration of silver fir forests.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers",
volume = "49",
number = "3",
pages = "979-988",
doi = "10.2298/GENSR1703979P",
url = "conv_970"
}
Popović, V., Lučić, A., Rakonjac, L., Mladenović-Drinić, S., Ristić, D.,& Cvjetković, B.. (2017). Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers. in Genetika
Društvo genetičara Srbije, Beograd., 49(3), 979-988.
https://doi.org/10.2298/GENSR1703979P
conv_970
Popović V, Lučić A, Rakonjac L, Mladenović-Drinić S, Ristić D, Cvjetković B. Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers. in Genetika. 2017;49(3):979-988.
doi:10.2298/GENSR1703979P
conv_970 .
Popović, Vladan, Lučić, Aleksandar, Rakonjac, Ljubinko, Mladenović-Drinić, Snežana, Ristić, Danijela, Cvjetković, Branislav, "Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers" in Genetika, 49, no. 3 (2017):979-988,
https://doi.org/10.2298/GENSR1703979P .,
conv_970 .
1
4
4

Maize landraces as a source of adaptation to climatic change

Anđelković, Violeta; Ristić, Danijela; Babić, Vojka; Dumanović, Zoran; Kravić, Natalija

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2016)

TY  - JOUR
AU  - Anđelković, Violeta
AU  - Ristić, Danijela
AU  - Babić, Vojka
AU  - Dumanović, Zoran
AU  - Kravić, Natalija
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/657
AB  - Predictions of global warming point out that frequency and severity of temperature and rainfall extremes are expected to increase in the following decades. Yield stability could be achieved by improvement in future breeding based on information of cereal reproduction under changed environmental conditions. Landraces are valuable sources of beneficial alleles, e.g. tolerance to drought and improved grain yield, for introgression into adapted germplasm. Twenty-six drought tolerant maize landraces, selected among gene bank accessions and previously tested under controlled drought conditions and in temperate climate, were evaluated, at two sites and two plant densities in Zemun Polje. Since 2014 was with enormous rainfalls and flooding, morphological and agronomical traits were measured. Obtained data, together with the results from dry 2012, were plotted for principal component analysis. One local landrace and two introduced landraces, medium in maturity, were selected according to PC analysis and achieved grain yield that was stable across different climatic conditions.
AB  - Procenjuje se da će u narednim decenijama, usled globalnog zagrevanja, doći do učestalije pojave ekstremnih temperatura i padavina. Ostvarivanje stabilnih prinosa u budućim programima oplemenjivanja zavisiće od informacija o mogućnostima reprodukcije useva u promenjenim uslovima spoljašnje sredine. Populacije predstavljaju dragocen izvor poželjnih alela (npr. za tolerantnost prema suši i poboljšanje prinosa) za introgresiju u adaptiranu germplazmu. Dvadeset šest populacija kukuruza, tolerantnih na sušu, odabranih iz banke gena i prethodno testiranih u uslovima kontrolisanog stresa suše, kao i u umerenom klimatu, testirane su na dva lokaliteta i u dve različite gustine u Zemun Polju. U 2014. godini koju su obeležile velike količine padavina i poplave, merena su morfološka i agronomska svojstva. Dobijeni podaci, zajedno sa podacima iz sušne 2012. godine obrađeni su PCA metodom (analiza glavnih komponenti). Jedna lokalna i dve introdukované, srednjestasne populacije, odabrane su na osnovu PC analize i ostvarenog prinosa zrna, koji je bio stabilan bez većih variranja, u različitim uslovima spoljašnje sredine.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Maize landraces as a source of adaptation to climatic change
T1  - Populacije kukuruza kao izvor adaptabilnosti na klimatske promene
VL  - 53
IS  - 1
SP  - 24
EP  - 29
DO  - 10.5937/ratpov53-9138
UR  - conv_621
ER  - 
@article{
author = "Anđelković, Violeta and Ristić, Danijela and Babić, Vojka and Dumanović, Zoran and Kravić, Natalija",
year = "2016",
abstract = "Predictions of global warming point out that frequency and severity of temperature and rainfall extremes are expected to increase in the following decades. Yield stability could be achieved by improvement in future breeding based on information of cereal reproduction under changed environmental conditions. Landraces are valuable sources of beneficial alleles, e.g. tolerance to drought and improved grain yield, for introgression into adapted germplasm. Twenty-six drought tolerant maize landraces, selected among gene bank accessions and previously tested under controlled drought conditions and in temperate climate, were evaluated, at two sites and two plant densities in Zemun Polje. Since 2014 was with enormous rainfalls and flooding, morphological and agronomical traits were measured. Obtained data, together with the results from dry 2012, were plotted for principal component analysis. One local landrace and two introduced landraces, medium in maturity, were selected according to PC analysis and achieved grain yield that was stable across different climatic conditions., Procenjuje se da će u narednim decenijama, usled globalnog zagrevanja, doći do učestalije pojave ekstremnih temperatura i padavina. Ostvarivanje stabilnih prinosa u budućim programima oplemenjivanja zavisiće od informacija o mogućnostima reprodukcije useva u promenjenim uslovima spoljašnje sredine. Populacije predstavljaju dragocen izvor poželjnih alela (npr. za tolerantnost prema suši i poboljšanje prinosa) za introgresiju u adaptiranu germplazmu. Dvadeset šest populacija kukuruza, tolerantnih na sušu, odabranih iz banke gena i prethodno testiranih u uslovima kontrolisanog stresa suše, kao i u umerenom klimatu, testirane su na dva lokaliteta i u dve različite gustine u Zemun Polju. U 2014. godini koju su obeležile velike količine padavina i poplave, merena su morfološka i agronomska svojstva. Dobijeni podaci, zajedno sa podacima iz sušne 2012. godine obrađeni su PCA metodom (analiza glavnih komponenti). Jedna lokalna i dve introdukované, srednjestasne populacije, odabrane su na osnovu PC analize i ostvarenog prinosa zrna, koji je bio stabilan bez većih variranja, u različitim uslovima spoljašnje sredine.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Maize landraces as a source of adaptation to climatic change, Populacije kukuruza kao izvor adaptabilnosti na klimatske promene",
volume = "53",
number = "1",
pages = "24-29",
doi = "10.5937/ratpov53-9138",
url = "conv_621"
}
Anđelković, V., Ristić, D., Babić, V., Dumanović, Z.,& Kravić, N.. (2016). Maize landraces as a source of adaptation to climatic change. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 53(1), 24-29.
https://doi.org/10.5937/ratpov53-9138
conv_621
Anđelković V, Ristić D, Babić V, Dumanović Z, Kravić N. Maize landraces as a source of adaptation to climatic change. in Ratarstvo i povrtarstvo. 2016;53(1):24-29.
doi:10.5937/ratpov53-9138
conv_621 .
Anđelković, Violeta, Ristić, Danijela, Babić, Vojka, Dumanović, Zoran, Kravić, Natalija, "Maize landraces as a source of adaptation to climatic change" in Ratarstvo i povrtarstvo, 53, no. 1 (2016):24-29,
https://doi.org/10.5937/ratpov53-9138 .,
conv_621 .
2

Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.)

Pavlov, Jovan; Delić, Nenad; Ristić, Danijela; Čamdžija, Zoran; Stevanović, Milan; Tolimir, Miodrag; Zivanović, Tomislav

(Društvo genetičara Srbije, Beograd, 2016)

TY  - JOUR
AU  - Pavlov, Jovan
AU  - Delić, Nenad
AU  - Ristić, Danijela
AU  - Čamdžija, Zoran
AU  - Stevanović, Milan
AU  - Tolimir, Miodrag
AU  - Zivanović, Tomislav
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/647
AB  - Six maize inbred lines for which we assumed that have a similar genetic background were selected for the study. Inbred lines were crossed according to incomplete diallel design and fifteen hybrid combinations were obtained. Hybrid combinations and their parental lines were used in our study. The objective of the study was to examine genetic polymorphism of parental lines, as well as to determine relationships between SSR genetic distance and values of high parent heterosis and specific combining abilities for grain yield. By using 19 SSR primers we classified inbred lines in two groups. First cluster consists of inbred lines L1, L2, L3, L4 and L5, while inbred line L6 formed second cluster, indicating different heterotic background in comparison to the other five inbred lines. The Spearman's correlation coefficients between SSR based genetic distance and specific combining abilities for grain yield had a value of (r=0,53*), while positive correlation were also found between genetic distance and high parent heterosis (r=0,57*).
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.)
VL  - 48
IS  - 1
SP  - 165
EP  - 172
DO  - 10.2298/GENSR1601165P
UR  - conv_929
ER  - 
@article{
author = "Pavlov, Jovan and Delić, Nenad and Ristić, Danijela and Čamdžija, Zoran and Stevanović, Milan and Tolimir, Miodrag and Zivanović, Tomislav",
year = "2016",
abstract = "Six maize inbred lines for which we assumed that have a similar genetic background were selected for the study. Inbred lines were crossed according to incomplete diallel design and fifteen hybrid combinations were obtained. Hybrid combinations and their parental lines were used in our study. The objective of the study was to examine genetic polymorphism of parental lines, as well as to determine relationships between SSR genetic distance and values of high parent heterosis and specific combining abilities for grain yield. By using 19 SSR primers we classified inbred lines in two groups. First cluster consists of inbred lines L1, L2, L3, L4 and L5, while inbred line L6 formed second cluster, indicating different heterotic background in comparison to the other five inbred lines. The Spearman's correlation coefficients between SSR based genetic distance and specific combining abilities for grain yield had a value of (r=0,53*), while positive correlation were also found between genetic distance and high parent heterosis (r=0,57*).",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.)",
volume = "48",
number = "1",
pages = "165-172",
doi = "10.2298/GENSR1601165P",
url = "conv_929"
}
Pavlov, J., Delić, N., Ristić, D., Čamdžija, Z., Stevanović, M., Tolimir, M.,& Zivanović, T.. (2016). Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.). in Genetika
Društvo genetičara Srbije, Beograd., 48(1), 165-172.
https://doi.org/10.2298/GENSR1601165P
conv_929
Pavlov J, Delić N, Ristić D, Čamdžija Z, Stevanović M, Tolimir M, Zivanović T. Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.). in Genetika. 2016;48(1):165-172.
doi:10.2298/GENSR1601165P
conv_929 .
Pavlov, Jovan, Delić, Nenad, Ristić, Danijela, Čamdžija, Zoran, Stevanović, Milan, Tolimir, Miodrag, Zivanović, Tomislav, "Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.)" in Genetika, 48, no. 1 (2016):165-172,
https://doi.org/10.2298/GENSR1601165P .,
conv_929 .
2
4
5

Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits

Kostadinović, Marija; Ignjatović-Micić, Dragana; Ristić, Danijela; Mladenović-Drinić, Snežana; Vančetović, Jelena; Božinović, Sofija; Stanković, Goran

(Public Library Science, San Francisco, 2016)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Stanković, Goran
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/621
AB  - Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.
PB  - Public Library Science, San Francisco
T2  - PLOS ONE
T1  - Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167635
UR  - conv_941
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Ristić, Danijela and Mladenović-Drinić, Snežana and Vančetović, Jelena and Božinović, Sofija and Stanković, Goran",
year = "2016",
abstract = "Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.",
publisher = "Public Library Science, San Francisco",
journal = "PLOS ONE",
title = "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167635",
url = "conv_941"
}
Kostadinović, M., Ignjatović-Micić, D., Ristić, D., Mladenović-Drinić, S., Vančetović, J., Božinović, S.,& Stanković, G.. (2016). Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167635
conv_941
Kostadinović M, Ignjatović-Micić D, Ristić D, Mladenović-Drinić S, Vančetović J, Božinović S, Stanković G. Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE. 2016;11(12).
doi:10.1371/journal.pone.0167635
conv_941 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Ristić, Danijela, Mladenović-Drinić, Snežana, Vančetović, Jelena, Božinović, Sofija, Stanković, Goran, "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits" in PLOS ONE, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167635 .,
conv_941 .
1
7
7
7

Grain yield and phenotypic performances of maize inbreds under variable environmental conditions

Kravić, Natalija; Babić, Vojka; Ristić, Danijela; Srdić, Jelena; Anđelković, Violeta

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2015)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Ristić, Danijela
AU  - Srdić, Jelena
AU  - Anđelković, Violeta
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/596
AB  - According to regional reports on climate, more frequent and severe droughts and floods are already apparent. Modern maize breeding for different purposes (e.g. drought tolerance) emphasizes the importance for improved maize ability to withstand adverse environmental conditions. Genotypes with drought tolerance obtain high grain yield under optimal conditions and lower, but stable yield under dry environmental conditions. Field testing was conducted in 2014, on twenty-three maize inbred lines differing in drought tolerance. The inbreds were evaluated in respect to morphological traits (plant and ear height, total number of leaves, number of leaves above uppermost ear, ear leaf length and width), yield components and grain yield per plant. The experiment was set up according to RCB design with two replications. To simulate water stress, two plant densities were applied. According to Principal Component Analysis and cluster analysis, five inbred lines (T1, T2, T4, T8 i T15) performed the highest stability in both experimental conditions. Those inbreds could be used as a source for drought tolerance in breeding programs.
AB  - Regionalna istraživanja klimatskih promena ukazuju na sve učestaliju pojavu promena u režimu padavina i temperatura. Za potrebe savremene selekcije za različite osobine (npr. tolerantnost prema suši), poboljšana sposobnost kukuruza da izdrži nepovoljne uslove spoljašnje sredine je esencijalna. U optimalnim uslovima, genotipovi tolerantni na sušu ostvaruju visok prinos, dok u uslovima vodnog deficita u kombinaciji sa visokim temperaturama vazduha ostvaruju niži, ali stabilan prinos. U poljskim ogledima 2014, izvršena je evaluacija dvadeset tri inbred linije kukuruza različite tolerantnosti na stres suše, praćenjem morfoloških osobina (visine biljke i gornjeg klipa, ukupnog broja listova i broja listova iznad gornjeg klipa, dižine i širine klipnog lista), komponenata prinosa i prinosa zrna po biljci. Ogled je sejan po kompletno randomiziranom blok dizajnu, u dva ponavljanja. Za simulaciju vodnog deficita, ogled je sejan u dve gustine. Poređenjem rezultata iz obe eksperimentalne gustine, dobijenih analizom glavnih komponenata i klaster analizom, pet samooplodnih linija kukuruza (T1, T2, T4, T8 i T15) su pokazale najveću stabilnost i mogu se smatrati poželjnim izvorima tolerantnosti prema suši za potrebe oplemenjivanja na ovo svojstvo.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Grain yield and phenotypic performances of maize inbreds under variable environmental conditions
T1  - Prinos i fenotipske karakteristike inbred linija kukuruza u promenljivim uslovima spoljašnje sredine
VL  - 21
IS  - 2
SP  - 1
EP  - 10
DO  - 10.5937/SelSem1502001K
UR  - conv_300
ER  - 
@article{
author = "Kravić, Natalija and Babić, Vojka and Ristić, Danijela and Srdić, Jelena and Anđelković, Violeta",
year = "2015",
abstract = "According to regional reports on climate, more frequent and severe droughts and floods are already apparent. Modern maize breeding for different purposes (e.g. drought tolerance) emphasizes the importance for improved maize ability to withstand adverse environmental conditions. Genotypes with drought tolerance obtain high grain yield under optimal conditions and lower, but stable yield under dry environmental conditions. Field testing was conducted in 2014, on twenty-three maize inbred lines differing in drought tolerance. The inbreds were evaluated in respect to morphological traits (plant and ear height, total number of leaves, number of leaves above uppermost ear, ear leaf length and width), yield components and grain yield per plant. The experiment was set up according to RCB design with two replications. To simulate water stress, two plant densities were applied. According to Principal Component Analysis and cluster analysis, five inbred lines (T1, T2, T4, T8 i T15) performed the highest stability in both experimental conditions. Those inbreds could be used as a source for drought tolerance in breeding programs., Regionalna istraživanja klimatskih promena ukazuju na sve učestaliju pojavu promena u režimu padavina i temperatura. Za potrebe savremene selekcije za različite osobine (npr. tolerantnost prema suši), poboljšana sposobnost kukuruza da izdrži nepovoljne uslove spoljašnje sredine je esencijalna. U optimalnim uslovima, genotipovi tolerantni na sušu ostvaruju visok prinos, dok u uslovima vodnog deficita u kombinaciji sa visokim temperaturama vazduha ostvaruju niži, ali stabilan prinos. U poljskim ogledima 2014, izvršena je evaluacija dvadeset tri inbred linije kukuruza različite tolerantnosti na stres suše, praćenjem morfoloških osobina (visine biljke i gornjeg klipa, ukupnog broja listova i broja listova iznad gornjeg klipa, dižine i širine klipnog lista), komponenata prinosa i prinosa zrna po biljci. Ogled je sejan po kompletno randomiziranom blok dizajnu, u dva ponavljanja. Za simulaciju vodnog deficita, ogled je sejan u dve gustine. Poređenjem rezultata iz obe eksperimentalne gustine, dobijenih analizom glavnih komponenata i klaster analizom, pet samooplodnih linija kukuruza (T1, T2, T4, T8 i T15) su pokazale najveću stabilnost i mogu se smatrati poželjnim izvorima tolerantnosti prema suši za potrebe oplemenjivanja na ovo svojstvo.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Grain yield and phenotypic performances of maize inbreds under variable environmental conditions, Prinos i fenotipske karakteristike inbred linija kukuruza u promenljivim uslovima spoljašnje sredine",
volume = "21",
number = "2",
pages = "1-10",
doi = "10.5937/SelSem1502001K",
url = "conv_300"
}
Kravić, N., Babić, V., Ristić, D., Srdić, J.,& Anđelković, V.. (2015). Grain yield and phenotypic performances of maize inbreds under variable environmental conditions. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 21(2), 1-10.
https://doi.org/10.5937/SelSem1502001K
conv_300
Kravić N, Babić V, Ristić D, Srdić J, Anđelković V. Grain yield and phenotypic performances of maize inbreds under variable environmental conditions. in Selekcija i semenarstvo. 2015;21(2):1-10.
doi:10.5937/SelSem1502001K
conv_300 .
Kravić, Natalija, Babić, Vojka, Ristić, Danijela, Srdić, Jelena, Anđelković, Violeta, "Grain yield and phenotypic performances of maize inbreds under variable environmental conditions" in Selekcija i semenarstvo, 21, no. 2 (2015):1-10,
https://doi.org/10.5937/SelSem1502001K .,
conv_300 .

A simple SSR analysis for genetic diversity estimation of maize landraces

Ignjatović-Micić, Dragana; Ristić, Danijela; Babić, Vojka; Anđelković, Violeta; Vančetović, Jelena

(Društvo genetičara Srbije, Beograd, 2015)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Babić, Vojka
AU  - Anđelković, Violeta
AU  - Vančetović, Jelena
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/567
AB  - A collection of 2217 landraces from western Balkan (former Yugoslavia) is maintained at Maize Research Institute Zemun Polje gene bank. Nine flint and nine dent accessions from six agro-ecological groups (races), chosen on the basis of diverse pedigrees, were analyzed for genetic relatedness using phenotypic and simple sequence repeat (SSR) markers. One of the aims was to establish a reliable set of SSR markers for a rapid diversity analysis using polyacrilamide gels and ethidium bromide staining. In the principal component analysis (PCA) the first three principal components accounted for 80.86% of total variation and separated most of the flint from dent landraces. Ten SSR primers revealed a total of 56 and 63 alleles in flint and dent landraces, respectively, with low stuttering and good allele resolution on the gels. High average PIC value (0.822) also supports informativeness and utility of the markers used in this study. Higher genetic variation was observed among flint genotypes, as genetic distances between flint landraces covered a larger range of values (0.11-0.38) than between dent (0.22 - 0.33) genotypes. Both phenotypic and SSR analyses distinguished flint and dent landraces, but neither of them could abstract agro-ecological groups. The SSR method used gave clear, easy to read band patterns that could be used for reliable allele frequency determination. Genetic diversity revealed for both markers indicated that the landraces were highly adapted to specific environmental conditions and purposes and could be valuable sources of genetic variability.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - A simple SSR analysis for genetic diversity estimation of maize landraces
VL  - 47
IS  - 1
SP  - 53
EP  - 62
DO  - 10.2298/GENSR1501053I
UR  - conv_905
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Ristić, Danijela and Babić, Vojka and Anđelković, Violeta and Vančetović, Jelena",
year = "2015",
abstract = "A collection of 2217 landraces from western Balkan (former Yugoslavia) is maintained at Maize Research Institute Zemun Polje gene bank. Nine flint and nine dent accessions from six agro-ecological groups (races), chosen on the basis of diverse pedigrees, were analyzed for genetic relatedness using phenotypic and simple sequence repeat (SSR) markers. One of the aims was to establish a reliable set of SSR markers for a rapid diversity analysis using polyacrilamide gels and ethidium bromide staining. In the principal component analysis (PCA) the first three principal components accounted for 80.86% of total variation and separated most of the flint from dent landraces. Ten SSR primers revealed a total of 56 and 63 alleles in flint and dent landraces, respectively, with low stuttering and good allele resolution on the gels. High average PIC value (0.822) also supports informativeness and utility of the markers used in this study. Higher genetic variation was observed among flint genotypes, as genetic distances between flint landraces covered a larger range of values (0.11-0.38) than between dent (0.22 - 0.33) genotypes. Both phenotypic and SSR analyses distinguished flint and dent landraces, but neither of them could abstract agro-ecological groups. The SSR method used gave clear, easy to read band patterns that could be used for reliable allele frequency determination. Genetic diversity revealed for both markers indicated that the landraces were highly adapted to specific environmental conditions and purposes and could be valuable sources of genetic variability.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "A simple SSR analysis for genetic diversity estimation of maize landraces",
volume = "47",
number = "1",
pages = "53-62",
doi = "10.2298/GENSR1501053I",
url = "conv_905"
}
Ignjatović-Micić, D., Ristić, D., Babić, V., Anđelković, V.,& Vančetović, J.. (2015). A simple SSR analysis for genetic diversity estimation of maize landraces. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 53-62.
https://doi.org/10.2298/GENSR1501053I
conv_905
Ignjatović-Micić D, Ristić D, Babić V, Anđelković V, Vančetović J. A simple SSR analysis for genetic diversity estimation of maize landraces. in Genetika. 2015;47(1):53-62.
doi:10.2298/GENSR1501053I
conv_905 .
Ignjatović-Micić, Dragana, Ristić, Danijela, Babić, Vojka, Anđelković, Violeta, Vančetović, Jelena, "A simple SSR analysis for genetic diversity estimation of maize landraces" in Genetika, 47, no. 1 (2015):53-62,
https://doi.org/10.2298/GENSR1501053I .,
conv_905 .
8
7
10

Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield

Božinović, Sofija; Vančetović, Jelena; Nikolić, Ana; Ristić, Danijela; Kostadinović, Marija; Ignjatović-Micić, Dragana; Prodanović, Slaven

(Inst Investigaciones Agropecuarias, Chillan, 2015)

TY  - JOUR
AU  - Božinović, Sofija
AU  - Vančetović, Jelena
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Prodanović, Slaven
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/588
AB  - Plus-hybrid effect refers to a combined effect of cytoplasmic male sterility (CMS) and xenia in maize (Zea mays L.) It could be used in commercial production by growing a mixture of 80% CMS hybrid and 20% of another fertile hybrid. The aim of this research was to examine individual and combined CMS and xenia effects on two hybrids widely grown in Serbia. Sterile and fertile versions of ZP 1 and ZP 2 hybrids (three-way; Iodent x Lancaster dents) were used as females, while ZP 1, ZP 2, ZP 3, ZP 4, and ZP 5 (three-way or single cross; Iodent (BSSS) x Lancaster dents) were used as pollinators. All of them belong to medium maturity group. The trial was set up at one location in Serbia (Zemun Polje) in 2009, 2010, and 2011. Molecular analysis of the five genotypes was done using simple sequence repeat (SSR) primers. Plus-hybrid effect on grain yield ranged from -6.2% to 6.2%; on thousand kernel weight from -1.7% to 5.2%; on number of kernels per area from -1.0% to 8.0%. The poor response could be due to a use of three-way instead of single cross hybrids in S type of sterility. Modified Rogers' distance between hybrids was in the range 0.211 to 0.378 and was not relevant for the effect, which depended mostly on the sterile hybrid genotype and the fertile hybrid pollinator ability. This approach should be more suitable for female hybrids with slightly poorer performance, already being produced on a sterile base.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield
VL  - 75
IS  - 2
SP  - 160
EP  - 167
DO  - 10.4067/S0718-58392015000200004
UR  - conv_913
ER  - 
@article{
author = "Božinović, Sofija and Vančetović, Jelena and Nikolić, Ana and Ristić, Danijela and Kostadinović, Marija and Ignjatović-Micić, Dragana and Prodanović, Slaven",
year = "2015",
abstract = "Plus-hybrid effect refers to a combined effect of cytoplasmic male sterility (CMS) and xenia in maize (Zea mays L.) It could be used in commercial production by growing a mixture of 80% CMS hybrid and 20% of another fertile hybrid. The aim of this research was to examine individual and combined CMS and xenia effects on two hybrids widely grown in Serbia. Sterile and fertile versions of ZP 1 and ZP 2 hybrids (three-way; Iodent x Lancaster dents) were used as females, while ZP 1, ZP 2, ZP 3, ZP 4, and ZP 5 (three-way or single cross; Iodent (BSSS) x Lancaster dents) were used as pollinators. All of them belong to medium maturity group. The trial was set up at one location in Serbia (Zemun Polje) in 2009, 2010, and 2011. Molecular analysis of the five genotypes was done using simple sequence repeat (SSR) primers. Plus-hybrid effect on grain yield ranged from -6.2% to 6.2%; on thousand kernel weight from -1.7% to 5.2%; on number of kernels per area from -1.0% to 8.0%. The poor response could be due to a use of three-way instead of single cross hybrids in S type of sterility. Modified Rogers' distance between hybrids was in the range 0.211 to 0.378 and was not relevant for the effect, which depended mostly on the sterile hybrid genotype and the fertile hybrid pollinator ability. This approach should be more suitable for female hybrids with slightly poorer performance, already being produced on a sterile base.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield",
volume = "75",
number = "2",
pages = "160-167",
doi = "10.4067/S0718-58392015000200004",
url = "conv_913"
}
Božinović, S., Vančetović, J., Nikolić, A., Ristić, D., Kostadinović, M., Ignjatović-Micić, D.,& Prodanović, S.. (2015). Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 75(2), 160-167.
https://doi.org/10.4067/S0718-58392015000200004
conv_913
Božinović S, Vančetović J, Nikolić A, Ristić D, Kostadinović M, Ignjatović-Micić D, Prodanović S. Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield. in Chilean Journal of Agricultural Research. 2015;75(2):160-167.
doi:10.4067/S0718-58392015000200004
conv_913 .
Božinović, Sofija, Vančetović, Jelena, Nikolić, Ana, Ristić, Danijela, Kostadinović, Marija, Ignjatović-Micić, Dragana, Prodanović, Slaven, "Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield" in Chilean Journal of Agricultural Research, 75, no. 2 (2015):160-167,
https://doi.org/10.4067/S0718-58392015000200004 .,
conv_913 .
6
3
4

Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers

Popović, Vladan; Rakonjac, Ljubinko; Lučić, Aleksandar; Ristić, Danijela; Mladenović-Drinić, Snežana; Hadrović, Sabahudin

(Društvo genetičara Srbije, Beograd, 2015)

TY  - JOUR
AU  - Popović, Vladan
AU  - Rakonjac, Ljubinko
AU  - Lučić, Aleksandar
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
AU  - Hadrović, Sabahudin
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/593
AB  - The analysis of Bald cypress genetic variability at the level of test trees was performed using RAPD (Random Amlified Polymorphic DNA) markers. RAPD analysis was performed on 20 test trees with 13 primers. A total of ten primers gave a clear picture while three primers amplified weakly. 60 is a total number of detected bands obtained by RAPD analysis with 10 selected primers, and the average number of bands is 6. Based on presence/absence of RAPD fragments among all 20 Bald cypress test trees were calculated similarity coefficients by Dice and they range from 0.73 to 1. Based on similarity coefficients was performed the cluster analysis and results were presented as a dendrogram. All 20 test trees were grouped into two sub-clusters. Test trees 1, 4 and 11 were grouped in the first sub-cluster while other test trees were grouped in the second sub-cluster. By analysis of relations within every sub-cluster and sub-sub-cluster the existence of genetic distances between observed test trees can be noticed. The greatest similarity is between test trees 2, 12, 15 and 18. The results of genetic similarity and distance between observed test trees indicate the overwhelming presence of genetic diversity.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers
VL  - 47
IS  - 2
SP  - 571
EP  - 580
DO  - 10.2298/GENSR1502571P
UR  - conv_917
ER  - 
@article{
author = "Popović, Vladan and Rakonjac, Ljubinko and Lučić, Aleksandar and Ristić, Danijela and Mladenović-Drinić, Snežana and Hadrović, Sabahudin",
year = "2015",
abstract = "The analysis of Bald cypress genetic variability at the level of test trees was performed using RAPD (Random Amlified Polymorphic DNA) markers. RAPD analysis was performed on 20 test trees with 13 primers. A total of ten primers gave a clear picture while three primers amplified weakly. 60 is a total number of detected bands obtained by RAPD analysis with 10 selected primers, and the average number of bands is 6. Based on presence/absence of RAPD fragments among all 20 Bald cypress test trees were calculated similarity coefficients by Dice and they range from 0.73 to 1. Based on similarity coefficients was performed the cluster analysis and results were presented as a dendrogram. All 20 test trees were grouped into two sub-clusters. Test trees 1, 4 and 11 were grouped in the first sub-cluster while other test trees were grouped in the second sub-cluster. By analysis of relations within every sub-cluster and sub-sub-cluster the existence of genetic distances between observed test trees can be noticed. The greatest similarity is between test trees 2, 12, 15 and 18. The results of genetic similarity and distance between observed test trees indicate the overwhelming presence of genetic diversity.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers",
volume = "47",
number = "2",
pages = "571-580",
doi = "10.2298/GENSR1502571P",
url = "conv_917"
}
Popović, V., Rakonjac, L., Lučić, A., Ristić, D., Mladenović-Drinić, S.,& Hadrović, S.. (2015). Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers. in Genetika
Društvo genetičara Srbije, Beograd., 47(2), 571-580.
https://doi.org/10.2298/GENSR1502571P
conv_917
Popović V, Rakonjac L, Lučić A, Ristić D, Mladenović-Drinić S, Hadrović S. Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers. in Genetika. 2015;47(2):571-580.
doi:10.2298/GENSR1502571P
conv_917 .
Popović, Vladan, Rakonjac, Ljubinko, Lučić, Aleksandar, Ristić, Danijela, Mladenović-Drinić, Snežana, Hadrović, Sabahudin, "Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers" in Genetika, 47, no. 2 (2015):571-580,
https://doi.org/10.2298/GENSR1502571P .,
conv_917 .
1
2
2

Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line

Kostadinović, Marija; Ignjatović-Micić, Dragana; Stanković, Goran; Vančetović, Jelena; Ristić, Danijela; Mladenović Drinić, Snežana

(Burgas : Sciences events, 2014)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/959
AB  - Quality protein maize (QPM) is the maize with increased levels of two essential amino acids, hard endosperm and good agronomic performances. The recessive opaque2 (o2) mutation increases lysine and tryptophan content in maize grains. It has recently become possible to use marker assisted selection (MAS) to accelerate selection for the o2 allele in QPM breeding process. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The results presented herein are the part of marker assisted backcross breeding program for incorporation of the o2 into maize inbred line. Marker selected o2 recessive homozygous BC2F2 individuals were subjected to the whole genome background selection, which identified five progenies with 83 to 94% recurrent parent genome content. BC2F3 families are being evaluated for desirable agronomic and biochemical traits in replicated trials and the best lines will represent the QPM version of the standard line.
PB  - Burgas : Sciences events
C3  - Journal of international scientific publications: agriculture & food
T1  - Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line
VL  - 2
SP  - 199
EP  - 205
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Vančetović, Jelena and Ristić, Danijela and Mladenović Drinić, Snežana",
year = "2014",
abstract = "Quality protein maize (QPM) is the maize with increased levels of two essential amino acids, hard endosperm and good agronomic performances. The recessive opaque2 (o2) mutation increases lysine and tryptophan content in maize grains. It has recently become possible to use marker assisted selection (MAS) to accelerate selection for the o2 allele in QPM breeding process. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The results presented herein are the part of marker assisted backcross breeding program for incorporation of the o2 into maize inbred line. Marker selected o2 recessive homozygous BC2F2 individuals were subjected to the whole genome background selection, which identified five progenies with 83 to 94% recurrent parent genome content. BC2F3 families are being evaluated for desirable agronomic and biochemical traits in replicated trials and the best lines will represent the QPM version of the standard line.",
publisher = "Burgas : Sciences events",
journal = "Journal of international scientific publications: agriculture & food",
title = "Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line",
volume = "2",
pages = "199-205"
}
Kostadinović, M., Ignjatović-Micić, D., Stanković, G., Vančetović, J., Ristić, D.,& Mladenović Drinić, S.. (2014). Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line. in Journal of international scientific publications: agriculture & food
Burgas : Sciences events., 2, 199-205.
Kostadinović M, Ignjatović-Micić D, Stanković G, Vančetović J, Ristić D, Mladenović Drinić S. Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line. in Journal of international scientific publications: agriculture & food. 2014;2:199-205..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Vančetović, Jelena, Ristić, Danijela, Mladenović Drinić, Snežana, "Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line" in Journal of international scientific publications: agriculture & food, 2 (2014):199-205.

Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize

Kostadinović, Marija; Ignjatović-Micić, Dragana; Stanković, Goran; Vančetović, Jelena; Ristić, Danijela; Mladenović-Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2014)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/526
AB  - Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize
VL  - 46
IS  - 2
SP  - 579
EP  - 590
DO  - 10.2298/GENSR1402579K
UR  - conv_888
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Vančetović, Jelena and Ristić, Danijela and Mladenović-Drinić, Snežana",
year = "2014",
abstract = "Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize",
volume = "46",
number = "2",
pages = "579-590",
doi = "10.2298/GENSR1402579K",
url = "conv_888"
}
Kostadinović, M., Ignjatović-Micić, D., Stanković, G., Vančetović, J., Ristić, D.,& Mladenović-Drinić, S.. (2014). Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika
Društvo genetičara Srbije, Beograd., 46(2), 579-590.
https://doi.org/10.2298/GENSR1402579K
conv_888
Kostadinović M, Ignjatović-Micić D, Stanković G, Vančetović J, Ristić D, Mladenović-Drinić S. Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika. 2014;46(2):579-590.
doi:10.2298/GENSR1402579K
conv_888 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Vančetović, Jelena, Ristić, Danijela, Mladenović-Drinić, Snežana, "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize" in Genetika, 46, no. 2 (2014):579-590,
https://doi.org/10.2298/GENSR1402579K .,
conv_888 .
1
1
1

Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers

Lučić, Aleksandar; Popović, Vladan; Rakonjac, Ljubinko; Ćirković-Mitrović, Tatjana; Nevenić, Marija; Ristić, Danijela; Mladenović-Drinić, Snežana

(Srpsko biološko društvo, Beograd, i dr., 2014)

TY  - JOUR
AU  - Lučić, Aleksandar
AU  - Popović, Vladan
AU  - Rakonjac, Ljubinko
AU  - Ćirković-Mitrović, Tatjana
AU  - Nevenić, Marija
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/537
AB  - This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mu g/mu l. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i. e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers
VL  - 66
IS  - 4
SP  - 1485
EP  - 1492
DO  - 10.2298/ABS1404485L
UR  - conv_891
ER  - 
@article{
author = "Lučić, Aleksandar and Popović, Vladan and Rakonjac, Ljubinko and Ćirković-Mitrović, Tatjana and Nevenić, Marija and Ristić, Danijela and Mladenović-Drinić, Snežana",
year = "2014",
abstract = "This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mu g/mu l. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i. e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers",
volume = "66",
number = "4",
pages = "1485-1492",
doi = "10.2298/ABS1404485L",
url = "conv_891"
}
Lučić, A., Popović, V., Rakonjac, L., Ćirković-Mitrović, T., Nevenić, M., Ristić, D.,& Mladenović-Drinić, S.. (2014). Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(4), 1485-1492.
https://doi.org/10.2298/ABS1404485L
conv_891
Lučić A, Popović V, Rakonjac L, Ćirković-Mitrović T, Nevenić M, Ristić D, Mladenović-Drinić S. Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers. in Archives of Biological Sciences. 2014;66(4):1485-1492.
doi:10.2298/ABS1404485L
conv_891 .
Lučić, Aleksandar, Popović, Vladan, Rakonjac, Ljubinko, Ćirković-Mitrović, Tatjana, Nevenić, Marija, Ristić, Danijela, Mladenović-Drinić, Snežana, "Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers" in Archives of Biological Sciences, 66, no. 4 (2014):1485-1492,
https://doi.org/10.2298/ABS1404485L .,
conv_891 .
1
4
4

Ispitivanje genetičke varijabilnosti lokalnih populacija kukuruza (Zea mays L.) molekularnim markerima

Ristić, Danijela

(Универзитет у Београду, Биолошки факултет, 2013)

TY  - THES
AU  - Ristić, Danijela
PY  - 2013
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=1116
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:7864/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=45152271
UR  - http://nardus.mpn.gov.rs/123456789/2162
UR  - http://rik.mrizp.rs/handle/123456789/759
AB  - Agronomski biodiverzitet je širok pojam koji uključuje sve komponente biološkog diverziteta od značaja za hranu i poljoprivredu. On predstavlja rezultat interakcije između genetičkih resursa, životne sredine i upravljanja sistemima i prakse koji čine poljoprivrednu proizvodnju. Biljni genetički resursi, smatraju se izuzetno značajnim u obezbeđivanju dovoljne količine hrane neophodne za ljudsku ishranu. Procenjuje se da danas ukupno 30 useva obezbeđuju 95% čovekovih potreba za hranom. Kukuruz je jedna od najznačajnijih useva koja se gaji širom sveta. Iako poseduje izuzetno veliku genetičku varijabilnost, u komercijalnoj upotrebi se nalazi svega oko 5% ukupne germplazme kukuruza, koja obezbeđuje visoke prinose.Banka gena Instituta za kukruz „Zemun Polje“ održava kolekciju od 2217 lokalnih populacija kukuruza klasifikovanih u 18 agroekoloških grupa, sakupljenih na prostoru bivše Jugoslavije. Ispitivanje diverziteta lokalnih populacija predstavlja osnovni preduslov za njihovu efikasnu klasifikaciju, čuvanje i korišćenje, i ima za cilj procenu genetičke raznovrsnosti i strukture populacijaIspitana je genetička varijabilnost 54 lokalne (po tri populacije svake agroekološke grupe) i 6 introdukovanih populacija kukuruza (po dve iz Francuske, Gruzije i Kine). Za ispitivanje genetičke varijabilnosti populacija korišćeno je 18 morfoloških osobina i 10 RAPD (Random Amlified Polymorphic DNA) i 10 SSR (Simple Sequence Repeat) markera. Na osnovu morfoloških osobina urađena je analiza varijanse i uočene su značajne razlike kod svih osobina za različite izvore variranja što govori o visokom stepenu fenotipskog diverziteta između populacija. Takođe, dobijene su visoke vredosti heritabilnosti u širem smislu (preko 0,6) za skoro sve osobine, osim dužine granatog dela metlice. Rezultati PCA analize su ukazali da lokalne populacije kukuruza mogu biti okarakterisane pomoću osobina kao što su rast biljke, osobine metlice i karakteristike zrna, a zapaženo je i veće grupisanje tvrdunaca/polutvrdunaca, odnosno poluzubana/zubana. Na osnovu morfoloških osobina i molekularnih markera, pomoću UPMGA metode dobijeni su klasteri, koristeći NTSYSpc statistički program. Morfološka, SSR i RAPD analiza nisu dovele do jasnog grupisanja lokalnih populacija prema poreklu, ali je uočeno delimično poklapanje između grupa populacija povezanih u kastere/subklaste i putevima introdukcije, odnosno njihovog nastanka od originalnih populacija...
AB  - Agricultural biodiversity is a broad term which includes all components of biological diversity of relevance to food and agriculture. It represents the result of interaction between genetic resources, environmental protection and both management systems and practices that make agricultural production. Plant genetic resources are considered to be very important in providing sufficient amounts of food for human consumption. It is estimated that today a total of 30 crops provide 95% of human needs for food. Corn is one of the most important crops that are grown around the world. Although it has a very high genetic variability, only about 5% of the germplasm is in the commercial use, which provides high yields.Maize Research Institute „Zemun Polje“ genebank maintains a collection of 2217 maize landraces classified into 18 agro-ecological groups, collected in the former Yugoslavia. Evaluation of genetic diversity of the local population represents basic precondition for their effective classification, storage and use. It aims to assess the genetic diversity and population structure.Assessment of genetic variability was done on 54 maize landraces (three landraces from each agro-ecological group) and six introduced maize landraces (two of each from France, Georgia and China). In order to analyze genetic variability of maize landraces, 18 morphological traits, 10 RAPD (Random Amplified Polymorphic DNA) and 10 SSR (Simple Sequence Repeat) markers were used. Analysis of variance was performed for evaluate morphological traits. Significant differences were observed for all traits for different sources of variation which indicates a high degree of phenotypic diversity between populations. Also, high broad-sense heritability (over 0.6) were obtained for almost all the traits except branched tassel length. The results of PCA analysis indicated that local maize populations can be characterized by traits such as plant growth, tassel traits and kernel characteristics. It was also observed the larger grouping of flint/semi-flint respectively to semident/dent. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method was applied for cluster analysis. All marker data analyses were performed using NTSYSpc statistical program...
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Ispitivanje genetičke varijabilnosti lokalnih populacija kukuruza (Zea mays L.) molekularnim markerima
T1  - Genetic variability of maize landraces (zea mays l.) assessed by molecular markers
UR  - https://hdl.handle.net/21.15107/rcub_nardus_2162
ER  - 
@phdthesis{
author = "Ristić, Danijela",
year = "2013",
abstract = "Agronomski biodiverzitet je širok pojam koji uključuje sve komponente biološkog diverziteta od značaja za hranu i poljoprivredu. On predstavlja rezultat interakcije između genetičkih resursa, životne sredine i upravljanja sistemima i prakse koji čine poljoprivrednu proizvodnju. Biljni genetički resursi, smatraju se izuzetno značajnim u obezbeđivanju dovoljne količine hrane neophodne za ljudsku ishranu. Procenjuje se da danas ukupno 30 useva obezbeđuju 95% čovekovih potreba za hranom. Kukuruz je jedna od najznačajnijih useva koja se gaji širom sveta. Iako poseduje izuzetno veliku genetičku varijabilnost, u komercijalnoj upotrebi se nalazi svega oko 5% ukupne germplazme kukuruza, koja obezbeđuje visoke prinose.Banka gena Instituta za kukruz „Zemun Polje“ održava kolekciju od 2217 lokalnih populacija kukuruza klasifikovanih u 18 agroekoloških grupa, sakupljenih na prostoru bivše Jugoslavije. Ispitivanje diverziteta lokalnih populacija predstavlja osnovni preduslov za njihovu efikasnu klasifikaciju, čuvanje i korišćenje, i ima za cilj procenu genetičke raznovrsnosti i strukture populacijaIspitana je genetička varijabilnost 54 lokalne (po tri populacije svake agroekološke grupe) i 6 introdukovanih populacija kukuruza (po dve iz Francuske, Gruzije i Kine). Za ispitivanje genetičke varijabilnosti populacija korišćeno je 18 morfoloških osobina i 10 RAPD (Random Amlified Polymorphic DNA) i 10 SSR (Simple Sequence Repeat) markera. Na osnovu morfoloških osobina urađena je analiza varijanse i uočene su značajne razlike kod svih osobina za različite izvore variranja što govori o visokom stepenu fenotipskog diverziteta između populacija. Takođe, dobijene su visoke vredosti heritabilnosti u širem smislu (preko 0,6) za skoro sve osobine, osim dužine granatog dela metlice. Rezultati PCA analize su ukazali da lokalne populacije kukuruza mogu biti okarakterisane pomoću osobina kao što su rast biljke, osobine metlice i karakteristike zrna, a zapaženo je i veće grupisanje tvrdunaca/polutvrdunaca, odnosno poluzubana/zubana. Na osnovu morfoloških osobina i molekularnih markera, pomoću UPMGA metode dobijeni su klasteri, koristeći NTSYSpc statistički program. Morfološka, SSR i RAPD analiza nisu dovele do jasnog grupisanja lokalnih populacija prema poreklu, ali je uočeno delimično poklapanje između grupa populacija povezanih u kastere/subklaste i putevima introdukcije, odnosno njihovog nastanka od originalnih populacija..., Agricultural biodiversity is a broad term which includes all components of biological diversity of relevance to food and agriculture. It represents the result of interaction between genetic resources, environmental protection and both management systems and practices that make agricultural production. Plant genetic resources are considered to be very important in providing sufficient amounts of food for human consumption. It is estimated that today a total of 30 crops provide 95% of human needs for food. Corn is one of the most important crops that are grown around the world. Although it has a very high genetic variability, only about 5% of the germplasm is in the commercial use, which provides high yields.Maize Research Institute „Zemun Polje“ genebank maintains a collection of 2217 maize landraces classified into 18 agro-ecological groups, collected in the former Yugoslavia. Evaluation of genetic diversity of the local population represents basic precondition for their effective classification, storage and use. It aims to assess the genetic diversity and population structure.Assessment of genetic variability was done on 54 maize landraces (three landraces from each agro-ecological group) and six introduced maize landraces (two of each from France, Georgia and China). In order to analyze genetic variability of maize landraces, 18 morphological traits, 10 RAPD (Random Amplified Polymorphic DNA) and 10 SSR (Simple Sequence Repeat) markers were used. Analysis of variance was performed for evaluate morphological traits. Significant differences were observed for all traits for different sources of variation which indicates a high degree of phenotypic diversity between populations. Also, high broad-sense heritability (over 0.6) were obtained for almost all the traits except branched tassel length. The results of PCA analysis indicated that local maize populations can be characterized by traits such as plant growth, tassel traits and kernel characteristics. It was also observed the larger grouping of flint/semi-flint respectively to semident/dent. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method was applied for cluster analysis. All marker data analyses were performed using NTSYSpc statistical program...",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Ispitivanje genetičke varijabilnosti lokalnih populacija kukuruza (Zea mays L.) molekularnim markerima, Genetic variability of maize landraces (zea mays l.) assessed by molecular markers",
url = "https://hdl.handle.net/21.15107/rcub_nardus_2162"
}
Ristić, D.. (2013). Ispitivanje genetičke varijabilnosti lokalnih populacija kukuruza (Zea mays L.) molekularnim markerima. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_2162
Ristić D. Ispitivanje genetičke varijabilnosti lokalnih populacija kukuruza (Zea mays L.) molekularnim markerima. in Универзитет у Београду. 2013;.
https://hdl.handle.net/21.15107/rcub_nardus_2162 .
Ristić, Danijela, "Ispitivanje genetičke varijabilnosti lokalnih populacija kukuruza (Zea mays L.) molekularnim markerima" in Универзитет у Београду (2013),
https://hdl.handle.net/21.15107/rcub_nardus_2162 .

Genetic assessment of maize landraces from former Yugoslavia

Ignjatović-Micić, Dragana; Ristić, Danijela; Babić, Vojka; Anđelković, Violeta; Marković, Ksenija; Vančetović, Jelena

(Društvo genetičara Srbije, Beograd, 2013)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Babić, Vojka
AU  - Anđelković, Violeta
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/519
AB  - A collection of 2217 landraces from former Yugoslavia region is maintained at Maize Research Institute Zemun Polje gene bank. All local varieties from the former Yugoslavia are classified into 18 groups. These agroecological groups encompass early introduced flint types grown on small and isolated areas, later introduced dent types that spread on wide areas of crop production and types created through hybridization between these two kernel types. The objective of this research was to study population structure, genetic diversity and relationships of nine flint and nine dent accessions belonging to different agro-ecological groups using phenotypic and simple sequence repeat (SSR) markers. The 18 analyzed landraces displayed great variation for most analyzed traits, but flint landraces were more diverse. Ten SSR probes revealed total of 56 and 62 alleles in flint and dent landraces, respectively. Eight specific alleles (i.e. alleles found only in one landrace or only within flint, i.e. dent landraces) were detected with five probes. One specific allele was found in flint and seven alleles in dent landraces. These differences in allele structure point to different origins and possibly different purposes of flint and dent genotypes. Both phenotypic and SSR analyses could distinguish most flint and dent landraces, but not agro-ecological groups. The results revealed a significant genetic heterogeneity indicating that the analyzed landraces could be valuable sources of genetic variability.
AB  - Kolekcija od 2217 lokalnih populacija sa teritorija Jugoslavije se održava u banci gena Instituta za kukuruz Zemun Polje. Populacije su klasifikovane u 18 agro-ekoloških grupa, koje obuhvataju rano introdikovane tipove tvrdunaca, kasnije inrodukovane tipove zubana i tipove nastale njihovom hibridizacijom. Cilj ovog rada je bio da se utvrdi struktura i genetička divergentnost, pomoću fenotipskih i SSR markera, devet populacija tvrdunaca i devet populacija zubana koji pripadaju različitim agro-ekološkim grupama. Analizirane populacije su pokazale visok stepen varijacija za većinu analiziranih osobina, mada su populacije tvrdunaca bile raznovrsnije. Ukupno 56 alela je detektovano u populacijama tvrdunaca, odnosno 64 u populacijama zubana, pomoću deset SSR markera. Osam specifičnih alela (alela detektovanih samo medu tvrduncima, odnosno zubanima) je identifikovano pomoću pet proba - jedan alel medu tvrduncima i sedam medu zubanima. Ove razlike u alelnoj strukturi ukazuju na razlicito poreklo i različite namene genotipova tvrdunaca i zubana. Fenotipska i SSR analiza su mogle da razdvoje većinu tvrdunaca od zubana, ali ne i agro-ekološke grupe. Rezultati su pokazali značajnu genetičku heterogenost analiziranih populacija, koja bi mogla biti dragocen izvor genetičke varijabilnosti.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic assessment of maize landraces from former Yugoslavia
T1  - Fenotipska i SSR analiza lokalnih populacija tvrdunaca i zubana iz regiona Jugoslavije
VL  - 45
IS  - 2
SP  - 405
EP  - 417
DO  - 10.2298/GENSR1302405I
UR  - conv_479
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Ristić, Danijela and Babić, Vojka and Anđelković, Violeta and Marković, Ksenija and Vančetović, Jelena",
year = "2013",
abstract = "A collection of 2217 landraces from former Yugoslavia region is maintained at Maize Research Institute Zemun Polje gene bank. All local varieties from the former Yugoslavia are classified into 18 groups. These agroecological groups encompass early introduced flint types grown on small and isolated areas, later introduced dent types that spread on wide areas of crop production and types created through hybridization between these two kernel types. The objective of this research was to study population structure, genetic diversity and relationships of nine flint and nine dent accessions belonging to different agro-ecological groups using phenotypic and simple sequence repeat (SSR) markers. The 18 analyzed landraces displayed great variation for most analyzed traits, but flint landraces were more diverse. Ten SSR probes revealed total of 56 and 62 alleles in flint and dent landraces, respectively. Eight specific alleles (i.e. alleles found only in one landrace or only within flint, i.e. dent landraces) were detected with five probes. One specific allele was found in flint and seven alleles in dent landraces. These differences in allele structure point to different origins and possibly different purposes of flint and dent genotypes. Both phenotypic and SSR analyses could distinguish most flint and dent landraces, but not agro-ecological groups. The results revealed a significant genetic heterogeneity indicating that the analyzed landraces could be valuable sources of genetic variability., Kolekcija od 2217 lokalnih populacija sa teritorija Jugoslavije se održava u banci gena Instituta za kukuruz Zemun Polje. Populacije su klasifikovane u 18 agro-ekoloških grupa, koje obuhvataju rano introdikovane tipove tvrdunaca, kasnije inrodukovane tipove zubana i tipove nastale njihovom hibridizacijom. Cilj ovog rada je bio da se utvrdi struktura i genetička divergentnost, pomoću fenotipskih i SSR markera, devet populacija tvrdunaca i devet populacija zubana koji pripadaju različitim agro-ekološkim grupama. Analizirane populacije su pokazale visok stepen varijacija za većinu analiziranih osobina, mada su populacije tvrdunaca bile raznovrsnije. Ukupno 56 alela je detektovano u populacijama tvrdunaca, odnosno 64 u populacijama zubana, pomoću deset SSR markera. Osam specifičnih alela (alela detektovanih samo medu tvrduncima, odnosno zubanima) je identifikovano pomoću pet proba - jedan alel medu tvrduncima i sedam medu zubanima. Ove razlike u alelnoj strukturi ukazuju na razlicito poreklo i različite namene genotipova tvrdunaca i zubana. Fenotipska i SSR analiza su mogle da razdvoje većinu tvrdunaca od zubana, ali ne i agro-ekološke grupe. Rezultati su pokazali značajnu genetičku heterogenost analiziranih populacija, koja bi mogla biti dragocen izvor genetičke varijabilnosti.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic assessment of maize landraces from former Yugoslavia, Fenotipska i SSR analiza lokalnih populacija tvrdunaca i zubana iz regiona Jugoslavije",
volume = "45",
number = "2",
pages = "405-417",
doi = "10.2298/GENSR1302405I",
url = "conv_479"
}
Ignjatović-Micić, D., Ristić, D., Babić, V., Anđelković, V., Marković, K.,& Vančetović, J.. (2013). Genetic assessment of maize landraces from former Yugoslavia. in Genetika
Društvo genetičara Srbije, Beograd., 45(2), 405-417.
https://doi.org/10.2298/GENSR1302405I
conv_479
Ignjatović-Micić D, Ristić D, Babić V, Anđelković V, Marković K, Vančetović J. Genetic assessment of maize landraces from former Yugoslavia. in Genetika. 2013;45(2):405-417.
doi:10.2298/GENSR1302405I
conv_479 .
Ignjatović-Micić, Dragana, Ristić, Danijela, Babić, Vojka, Anđelković, Violeta, Marković, Ksenija, Vančetović, Jelena, "Genetic assessment of maize landraces from former Yugoslavia" in Genetika, 45, no. 2 (2013):405-417,
https://doi.org/10.2298/GENSR1302405I .,
conv_479 .
5
5
6

Genetic diversity in maize dent landraces assessed by morphological and molecular markers

Ristić, Danijela; Babić, Vojka; Anđelković, Violeta; Vančetović, Jelena; Mladenović-Drinić, Snežana; Ignjatović-Micić, Dragana

(Društvo genetičara Srbije, Beograd, 2013)

TY  - JOUR
AU  - Ristić, Danijela
AU  - Babić, Vojka
AU  - Anđelković, Violeta
AU  - Vančetović, Jelena
AU  - Mladenović-Drinić, Snežana
AU  - Ignjatović-Micić, Dragana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/492
AB  - Maize Research Institute 'Zemun Polje' genebank maintains a collection of landraces grouped into 18 agro-ecological collected from ex-Yugoslavia territories. The application and comparison of different marker systems are important for the characterization and use of maize landraces in breeding program, as potential sources of desirable traits. In this study, 15 morphological traits, 7 RAPD primers and 10 SSR primer pairs were applied to i) to determine genetic distance between 21 maize dent landraces and ii) compare results obtained on morphological and molecular markers. Phenotypic analysis showed high level of heterogeneity between landraces. Higher level of genetic diversity was obtained with SSR than with RAPD. Genetic distance mean value for RAPD data was 0.35 i.e. for SSR 0.48. Based on the morphological traits and molecular markers, unweighted pairgroup method (UPGMA) analysis was applied for cluster analysis, using statistical NTSYSpc program package. Cluster analysis of morphological and molecular markers distances did not show the same population grouping. Better agreement with agro-ecological data was obtained with RAPD markers. Correlations between dissimilarity matrices for different types of markers were low. Data obtained in this work could be useful for further study of a larger number of landraces, and conservation of genetic resources and their genetic diversity.
AB  - Banka gena Institut za kukuruz 'Zemun Polje' održava kolekciju lokalnih populacija grupisanih u 18 agro-ekološka prikupljenih na prostoru bivše Jugoslavije. Primena i upoređivanje različitih marker-analiza su važni za karakterizaciju i korišćenje lokalnih populacija kukuruza u selekcionim programima, kao potencijalnih izvora poželjnih osobina. U ovom radu 15 morfoloških osobina, 7 RAPD prajmera i 10 SSR prajmera su primenjeni sa ciljem i) utvrđivanja genetičke udaljenosti između 21 lokalnih populacija kukuruza zubana i ii) upoređivanja rezultata dobijenih na osnovu morfoloških i molekularnih markera. Fenotipska analiza je pokazala visok stepen heterogenosti između lokalnih populacija. Viši nivo genetičkog diverziteta je dobijen sa SSR nego sa RAPD markerima. Srednja vrednost genetičke distance za RAPD je bila 0.35, odnosno 0.48 za SSR. Na osnovu morfoloških osobina i molekularnih markera, pomoću UPMGA analize dobijeni su klasteri, koristeći NTSYSpc statistički program. Klaster analiza na osnovu morfoloških i molekularnih markera nije pokazala isto grupisanje lokalnih populacija. Bolje slaganje sa agroekološkim podacima je dobijeno na osnovu RAPD markera. Korelacije su bile niske između genetičkih distanci za različite tipove markera. Rezultati dobijeni u ovom radu mogu biti od koristi za dalje proučavanje većeg broja lokalnih populacija, kao i očuvanje genetičkih resursa i njihovog genetičkog diverziteta.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic diversity in maize dent landraces assessed by morphological and molecular markers
T1  - Procena genetičke varijabilnosti lokalnih populacija zubana primenom morfoloških i molekularnih markera
VL  - 45
IS  - 3
SP  - 811
EP  - 824
DO  - 10.2298/GENSR1303811R
UR  - conv_486
ER  - 
@article{
author = "Ristić, Danijela and Babić, Vojka and Anđelković, Violeta and Vančetović, Jelena and Mladenović-Drinić, Snežana and Ignjatović-Micić, Dragana",
year = "2013",
abstract = "Maize Research Institute 'Zemun Polje' genebank maintains a collection of landraces grouped into 18 agro-ecological collected from ex-Yugoslavia territories. The application and comparison of different marker systems are important for the characterization and use of maize landraces in breeding program, as potential sources of desirable traits. In this study, 15 morphological traits, 7 RAPD primers and 10 SSR primer pairs were applied to i) to determine genetic distance between 21 maize dent landraces and ii) compare results obtained on morphological and molecular markers. Phenotypic analysis showed high level of heterogeneity between landraces. Higher level of genetic diversity was obtained with SSR than with RAPD. Genetic distance mean value for RAPD data was 0.35 i.e. for SSR 0.48. Based on the morphological traits and molecular markers, unweighted pairgroup method (UPGMA) analysis was applied for cluster analysis, using statistical NTSYSpc program package. Cluster analysis of morphological and molecular markers distances did not show the same population grouping. Better agreement with agro-ecological data was obtained with RAPD markers. Correlations between dissimilarity matrices for different types of markers were low. Data obtained in this work could be useful for further study of a larger number of landraces, and conservation of genetic resources and their genetic diversity., Banka gena Institut za kukuruz 'Zemun Polje' održava kolekciju lokalnih populacija grupisanih u 18 agro-ekološka prikupljenih na prostoru bivše Jugoslavije. Primena i upoređivanje različitih marker-analiza su važni za karakterizaciju i korišćenje lokalnih populacija kukuruza u selekcionim programima, kao potencijalnih izvora poželjnih osobina. U ovom radu 15 morfoloških osobina, 7 RAPD prajmera i 10 SSR prajmera su primenjeni sa ciljem i) utvrđivanja genetičke udaljenosti između 21 lokalnih populacija kukuruza zubana i ii) upoređivanja rezultata dobijenih na osnovu morfoloških i molekularnih markera. Fenotipska analiza je pokazala visok stepen heterogenosti između lokalnih populacija. Viši nivo genetičkog diverziteta je dobijen sa SSR nego sa RAPD markerima. Srednja vrednost genetičke distance za RAPD je bila 0.35, odnosno 0.48 za SSR. Na osnovu morfoloških osobina i molekularnih markera, pomoću UPMGA analize dobijeni su klasteri, koristeći NTSYSpc statistički program. Klaster analiza na osnovu morfoloških i molekularnih markera nije pokazala isto grupisanje lokalnih populacija. Bolje slaganje sa agroekološkim podacima je dobijeno na osnovu RAPD markera. Korelacije su bile niske između genetičkih distanci za različite tipove markera. Rezultati dobijeni u ovom radu mogu biti od koristi za dalje proučavanje većeg broja lokalnih populacija, kao i očuvanje genetičkih resursa i njihovog genetičkog diverziteta.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic diversity in maize dent landraces assessed by morphological and molecular markers, Procena genetičke varijabilnosti lokalnih populacija zubana primenom morfoloških i molekularnih markera",
volume = "45",
number = "3",
pages = "811-824",
doi = "10.2298/GENSR1303811R",
url = "conv_486"
}
Ristić, D., Babić, V., Anđelković, V., Vančetović, J., Mladenović-Drinić, S.,& Ignjatović-Micić, D.. (2013). Genetic diversity in maize dent landraces assessed by morphological and molecular markers. in Genetika
Društvo genetičara Srbije, Beograd., 45(3), 811-824.
https://doi.org/10.2298/GENSR1303811R
conv_486
Ristić D, Babić V, Anđelković V, Vančetović J, Mladenović-Drinić S, Ignjatović-Micić D. Genetic diversity in maize dent landraces assessed by morphological and molecular markers. in Genetika. 2013;45(3):811-824.
doi:10.2298/GENSR1303811R
conv_486 .
Ristić, Danijela, Babić, Vojka, Anđelković, Violeta, Vančetović, Jelena, Mladenović-Drinić, Snežana, Ignjatović-Micić, Dragana, "Genetic diversity in maize dent landraces assessed by morphological and molecular markers" in Genetika, 45, no. 3 (2013):811-824,
https://doi.org/10.2298/GENSR1303811R .,
conv_486 .
5
7
7

Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia

Lučić, Aleksandar; Cvjetićanin, Rade; Novaković-Vuković, Marijana; Ristić, Danijela; Popović, Vladan; Rakonjac, Ljubinko; Mladenović-Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2013)

TY  - JOUR
AU  - Lučić, Aleksandar
AU  - Cvjetićanin, Rade
AU  - Novaković-Vuković, Marijana
AU  - Ristić, Danijela
AU  - Popović, Vladan
AU  - Rakonjac, Ljubinko
AU  - Mladenović-Drinić, Snežana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/515
AB  - Multidisciplinary studies (genetic and phytocoenological) of 4 populations of Austrian pine (Pinus nigra Arnold) were carried out in western and central Serbia. The obtained results gave the same inter-population arrangement in both methods. Dendograms (NTSYS) differentiate at the greatest genetic distance the population of Austrian pine in Šargan compared to other populations: Crni Vrh, Goč and Studenica. Using phytocoenological analysis it was determined that population of Austrian pine in Šargan forms a community Erico-Pinetum gocensis, while the other populations form a community Seslerio rigidae-Pinetum gocensis. Multidisciplinary approach that was demonstrated within this paper presents the first studies of Austrian pine that directly link genotype dependence and environmental conditions manifested through the phytocoenological affiliation.
AB  - Multidisciplinarni pristup demostriran u okviru ovog rada predstavlja prva istraživanja na crnom boru koje u direktnu vezu dovodi zavisnost genotipa i uslove sredine ispoljene kroz fitocenološku pripadnost. U radu su izvršena multidisciplinarna istraživanja (genetička i fitocenološka) 4 populacije crnog bora (Pinus nigra Arnold) u zapadnoj i centralnoj Srbiji. Izvršena je analiza PCR amplifikacija genomske DNA sa 14 RAPD primera, od čega je 10 polimorfno. Korišćene su tri vrste koeficienata genetičke sličnosti, Jaccard, Dice and Socal and Michener. Dobijeni dendogrami (NTSYS) diferenciraju na najvećoj genetičkoj distanci populaciju crnog bora na Šarganu u odnosu na ostale populacije Crni Vrh, Goč i Studenica. Fitocenološke analize su vršene po metodi Braun-Blanquet i tom prilikom je utvrđeno da populacija crnog bora na Šarganu gradi zajednicu crnog bora sa crnjušom (Erico-Pinetum gocensis), a ostale populacije formiraju zajednice crnog bora sa uskolisnom šašikom (Seslerio rigidae-Pinetum gocensis). Dobijenih rezultata korišćenjem genetičke analize (PCR) i analize fitocenološke pripadnosti dali su isti međupopulacioni raspored.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia
T1  - Međupopulaciona varijabilnost crnog bora (Pinus nigra Arnold) u Srbiji
VL  - 45
IS  - 3
SP  - 641
EP  - 654
DO  - 10.2298/GENSR1303641L
UR  - conv_483
ER  - 
@article{
author = "Lučić, Aleksandar and Cvjetićanin, Rade and Novaković-Vuković, Marijana and Ristić, Danijela and Popović, Vladan and Rakonjac, Ljubinko and Mladenović-Drinić, Snežana",
year = "2013",
abstract = "Multidisciplinary studies (genetic and phytocoenological) of 4 populations of Austrian pine (Pinus nigra Arnold) were carried out in western and central Serbia. The obtained results gave the same inter-population arrangement in both methods. Dendograms (NTSYS) differentiate at the greatest genetic distance the population of Austrian pine in Šargan compared to other populations: Crni Vrh, Goč and Studenica. Using phytocoenological analysis it was determined that population of Austrian pine in Šargan forms a community Erico-Pinetum gocensis, while the other populations form a community Seslerio rigidae-Pinetum gocensis. Multidisciplinary approach that was demonstrated within this paper presents the first studies of Austrian pine that directly link genotype dependence and environmental conditions manifested through the phytocoenological affiliation., Multidisciplinarni pristup demostriran u okviru ovog rada predstavlja prva istraživanja na crnom boru koje u direktnu vezu dovodi zavisnost genotipa i uslove sredine ispoljene kroz fitocenološku pripadnost. U radu su izvršena multidisciplinarna istraživanja (genetička i fitocenološka) 4 populacije crnog bora (Pinus nigra Arnold) u zapadnoj i centralnoj Srbiji. Izvršena je analiza PCR amplifikacija genomske DNA sa 14 RAPD primera, od čega je 10 polimorfno. Korišćene su tri vrste koeficienata genetičke sličnosti, Jaccard, Dice and Socal and Michener. Dobijeni dendogrami (NTSYS) diferenciraju na najvećoj genetičkoj distanci populaciju crnog bora na Šarganu u odnosu na ostale populacije Crni Vrh, Goč i Studenica. Fitocenološke analize su vršene po metodi Braun-Blanquet i tom prilikom je utvrđeno da populacija crnog bora na Šarganu gradi zajednicu crnog bora sa crnjušom (Erico-Pinetum gocensis), a ostale populacije formiraju zajednice crnog bora sa uskolisnom šašikom (Seslerio rigidae-Pinetum gocensis). Dobijenih rezultata korišćenjem genetičke analize (PCR) i analize fitocenološke pripadnosti dali su isti međupopulacioni raspored.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia, Međupopulaciona varijabilnost crnog bora (Pinus nigra Arnold) u Srbiji",
volume = "45",
number = "3",
pages = "641-654",
doi = "10.2298/GENSR1303641L",
url = "conv_483"
}
Lučić, A., Cvjetićanin, R., Novaković-Vuković, M., Ristić, D., Popović, V., Rakonjac, L.,& Mladenović-Drinić, S.. (2013). Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 45(3), 641-654.
https://doi.org/10.2298/GENSR1303641L
conv_483
Lučić A, Cvjetićanin R, Novaković-Vuković M, Ristić D, Popović V, Rakonjac L, Mladenović-Drinić S. Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia. in Genetika. 2013;45(3):641-654.
doi:10.2298/GENSR1303641L
conv_483 .
Lučić, Aleksandar, Cvjetićanin, Rade, Novaković-Vuković, Marijana, Ristić, Danijela, Popović, Vladan, Rakonjac, Ljubinko, Mladenović-Drinić, Snežana, "Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia" in Genetika, 45, no. 3 (2013):641-654,
https://doi.org/10.2298/GENSR1303641L .,
conv_483 .
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