TY  - JOUR
AU  - Župunski, Vesna
AU  - Jevtić, Radivoje
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Ivanović, D.
AU  - Nikolić, Ana
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/414
AB  - With the growing importance of organic production and resurgence of bunt fungi in organic farming conditions, the present study was undertaken to determine incidence and contamination level of Tilletia species in conventionally produced non-processed seed of wheat in Vojvodina. Analysis of 151 samples of basic, certified and commercial seed revealed that 129 samples were contaminated with Tilletia species. The predominant species was T. caries, which was found in 127 samples. However, teliospores of Tilletia species, which have not been registered in Serbia before, were found in five samples. These teliospores had a prominent gelatinous sheath together with conspicuous, deep reticulation and it was assumed that some of them might be of the quarantined T. contraversa, whilst others were identified as T. bromi. Contamination level of basic and certified seed was about one teliospore per 10 seeds, while four out of 16 commercial seed samples were contaminated with more than 900 teliospores per seed which exceeds the allowed contamination level.
PB  - Ista-Int Seed Testing Assoc, Bassersdorf
T2  - Seed Science and Technology
T1  - Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia
VL  - 40
IS  - 3
SP  - 320
EP  - 332
DO  - 10.15258/sst.2012.40.3.04
ER  - 

@article{
author = "Župunski, Vesna and Jevtić, Radivoje and Ignjatović-Micić, Dragana and Stanković, Slavica and Lević, Jelena and Ivanović, D. and Nikolić, Ana",
year = "2012",
abstract = "With the growing importance of organic production and resurgence of bunt fungi in organic farming conditions, the present study was undertaken to determine incidence and contamination level of Tilletia species in conventionally produced non-processed seed of wheat in Vojvodina. Analysis of 151 samples of basic, certified and commercial seed revealed that 129 samples were contaminated with Tilletia species. The predominant species was T. caries, which was found in 127 samples. However, teliospores of Tilletia species, which have not been registered in Serbia before, were found in five samples. These teliospores had a prominent gelatinous sheath together with conspicuous, deep reticulation and it was assumed that some of them might be of the quarantined T. contraversa, whilst others were identified as T. bromi. Contamination level of basic and certified seed was about one teliospore per 10 seeds, while four out of 16 commercial seed samples were contaminated with more than 900 teliospores per seed which exceeds the allowed contamination level.",
publisher = "Ista-Int Seed Testing Assoc, Bassersdorf",
journal = "Seed Science and Technology",
title = "Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia",
volume = "40",
number = "3",
pages = "320-332",
doi = "10.15258/sst.2012.40.3.04"
}

Župunski, V., Jevtić, R., Ignjatović-Micić, D., Stanković, S., Lević, J., Ivanović, D.,& Nikolić, A.. (2012). Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia. in Seed Science and Technology
Ista-Int Seed Testing Assoc, Bassersdorf., 40(3), 320-332.
https://doi.org/10.15258/sst.2012.40.3.04

Župunski V, Jevtić R, Ignjatović-Micić D, Stanković S, Lević J, Ivanović D, Nikolić A. Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia. in Seed Science and Technology. 2012;40(3):320-332.
doi:10.15258/sst.2012.40.3.04 .

Župunski, Vesna, Jevtić, Radivoje, Ignjatović-Micić, Dragana, Stanković, Slavica, Lević, Jelena, Ivanović, D., Nikolić, Ana, "Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia" in Seed Science and Technology, 40, no. 3 (2012):320-332,
https://doi.org/10.15258/sst.2012.40.3.04 . .

TY  - JOUR
AU  - Župunski, Vesna
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Ana
AU  - Stanković, Slavica
AU  - Jevtić, Radivoje
AU  - Lević, Jelena
AU  - Ivanović, Dragica
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/370
AB  - Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %) samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas.
AB  - Istraživanjem kontaminiranosti uzoraka semena pšenice teleutosporama Tilletia vrsta, utvrđeno je da je od 167 uzoraka nedorađenog semena pšenice, bilo kontaminirano 145 (86,8%), dok su 22 uzorka (13,2%) smatrana nekontaminiranim. Identifikacija Tilletia vrsta izvršena je rep-PCR fingerprinting tehnikom. Izolati prikupljeni na teritoriji Republike Srbije identifikovani su kao T. tritici, s obzirom da su sa pozitivnom kontrolom imali genetičku sličnost veću od 80%. Jedan od izolata koji je vodio poreklo iz opštine Apatin bio je oko 60% genetički sličan sa izolatima T. tritici, T. controversa i T. laevis. Pretpostavljeno je da pripada vrsti T. bromi. Genetička sličnost izolata T. walkeri i vrsta: T. tritici, T. laevis i T. controversa, iznosila je nešto više od 10%. Genetička sličnost T. tritici, T. controversa i T. laevis bila je oko 70 %. I pored visokog procenta genetičke sličnosti između T. controversa i T. tritici, napravljena je razlika među njima, što rep-PCR fingerprinting tehniku čini veoma podesnom za praćenje prisustva morfološki sličnih Tilleria vrsta prilikom kontrole kvaliteta semena pšenice.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Identification of Tilletia species using rep-PCR fingerprinting technique
T1  - Identifikacija vrsta roda Tilletia rep-PCR fingerprinting tehnikom
VL  - 43
IS  - 1
SP  - 183
EP  - 195
DO  - 10.2298/GENSR1101183Z
ER  - 

@article{
author = "Župunski, Vesna and Ignjatović-Micić, Dragana and Nikolić, Ana and Stanković, Slavica and Jevtić, Radivoje and Lević, Jelena and Ivanović, Dragica",
year = "2011",
abstract = "Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %) samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas., Istraživanjem kontaminiranosti uzoraka semena pšenice teleutosporama Tilletia vrsta, utvrđeno je da je od 167 uzoraka nedorađenog semena pšenice, bilo kontaminirano 145 (86,8%), dok su 22 uzorka (13,2%) smatrana nekontaminiranim. Identifikacija Tilletia vrsta izvršena je rep-PCR fingerprinting tehnikom. Izolati prikupljeni na teritoriji Republike Srbije identifikovani su kao T. tritici, s obzirom da su sa pozitivnom kontrolom imali genetičku sličnost veću od 80%. Jedan od izolata koji je vodio poreklo iz opštine Apatin bio je oko 60% genetički sličan sa izolatima T. tritici, T. controversa i T. laevis. Pretpostavljeno je da pripada vrsti T. bromi. Genetička sličnost izolata T. walkeri i vrsta: T. tritici, T. laevis i T. controversa, iznosila je nešto više od 10%. Genetička sličnost T. tritici, T. controversa i T. laevis bila je oko 70 %. I pored visokog procenta genetičke sličnosti između T. controversa i T. tritici, napravljena je razlika među njima, što rep-PCR fingerprinting tehniku čini veoma podesnom za praćenje prisustva morfološki sličnih Tilleria vrsta prilikom kontrole kvaliteta semena pšenice.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Identification of Tilletia species using rep-PCR fingerprinting technique, Identifikacija vrsta roda Tilletia rep-PCR fingerprinting tehnikom",
volume = "43",
number = "1",
pages = "183-195",
doi = "10.2298/GENSR1101183Z"
}

Župunski, V., Ignjatović-Micić, D., Nikolić, A., Stanković, S., Jevtić, R., Lević, J.,& Ivanović, D.. (2011). Identification of Tilletia species using rep-PCR fingerprinting technique. in Genetika
Društvo genetičara Srbije, Beograd., 43(1), 183-195.
https://doi.org/10.2298/GENSR1101183Z

Župunski V, Ignjatović-Micić D, Nikolić A, Stanković S, Jevtić R, Lević J, Ivanović D. Identification of Tilletia species using rep-PCR fingerprinting technique. in Genetika. 2011;43(1):183-195.
doi:10.2298/GENSR1101183Z .

Župunski, Vesna, Ignjatović-Micić, Dragana, Nikolić, Ana, Stanković, Slavica, Jevtić, Radivoje, Lević, Jelena, Ivanović, Dragica, "Identification of Tilletia species using rep-PCR fingerprinting technique" in Genetika, 43, no. 1 (2011):183-195,
https://doi.org/10.2298/GENSR1101183Z . .