Delić, Nenad

Link to this page

Authority KeyName Variants
orcid::0000-0003-4020-0114
  • Delić, Nenad (92)
  • Delic, Nenad (1)
  • Delić, N. S. (1)
Projects
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200040 (Maize Research Institute 'Zemun Polje', Belgrade-Zemun) Improvment of maize and soybean traits by molecular and conventional breeding
Exploitation of maize diversity to improve grain quality and drought tolerance Utilization of plant sources of protein, dietary fiber and antioxidants in food production
Integrated field crop production: conservation of biodiversity and soil fertility Agricultural Experiment Station - 6699
COST action (Infogest) - FA1005 Dokaz koncepta (ID 5679), Inovacioni fond, Srbija
Evaluation of nutritional and financial effects of Quality Protein Maize (QPM) meal for broiler chickens (5679), The Proof-of-Concept Program, The Innovation Fund, Belgrade, Serbia Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200116 (University of Belgrade, Faculty of Agriculture) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200117 (University of Novi Sad, Faculty of Agriculture)
Fizička hemija dinamičkih stanja i struktura neravnotežnih sistema - od monotone do oscilatorne evolucije i haosa Razvoj tehnologije gajenja kukuruza sa ekološkim pristupom
Identifikacija izvora tolerantnosti prema suši u gen banci kukuruza Uticaj citoplazmatičke muške sterilnosti i ksenija na prinos zrna i agronomske osobine kukuruza
Stvaranje linija i hibrida kukuruza visokog kvaliteta proteina Identifikacija genotipova kukuruza i soje za proizvodnju hrane i biogorivo
Ministry of Education, Science and Technological Development of the Republic of Serbia, grant number 451-03-68/2022-14/200116 Projekt 101059942 — CREDIT Vibes (2023-2025)
Serbian Ministry of Education, Science and Technological Development Supported by GVM from Russian Science Foundation (grant No: 19-79-30062) as well as the Interdisciplinary Scientific and Educational School of Moscow University “Molecular Technologies of Living Systems and Synthetic Biology”
Transnational Access Module of the European Plant Phenotyping Network, FP7 Research Infrastructures Program of the European Union (EPPN, No. 284443). DD В Россий-ской Федерации исследования проводятся под руководством профессора Г.В. Максимова и финансиру-ются проектом РНФ ¹ 19-79-30062. Исследование также было поддержано Междисциплинарной научно-образовательной школой Московского государственного университета им. М.В. Ломоносова «Молекуляр-ные технологии живых систем и синтетическая биология».
В Сербии исследования финансировались Исследовательским институтом кукурузы «Земун Поле» (Бел-град) и Факультетом физической химии Белградского университета, а также Министерством образования, науки и технологического развития Сербии (Projects 03E211, 03E22)

Author's Bibliography

Grain yield stability estimates of late maturity maize hybrids

Pavlov, Jovan; Delić, Nenad; Čamdžija, Zoran; Branković, Gordana; Milosavljević ex Glišović, Nataša; Grčić, Nikola; Božinović, Sofija

(Beograd : Društvo genetičara Srbije, 2023)

TY  - JOUR
AU  - Pavlov, Jovan
AU  - Delić, Nenad
AU  - Čamdžija, Zoran
AU  - Branković, Gordana
AU  - Milosavljević ex Glišović, Nataša
AU  - Grčić, Nikola
AU  - Božinović, Sofija
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1304
AB  - With global climate change including unpredictable geographic and temporal weather
patterns that lead to significant genotype × environment interaction (GEI) the maize
performance assessment would need to be complemented with stability analysis. The
objectives of this study were: i) estimation of parametric and non-parametric stability
indices for grain yield of late maturity maize hybrids, ii) assessing correlations and
grouping of stability indices for grain yield of late maturity maize hybrids. The eight
maize hybrids of FAO 700 maturity group (ZP1-ZP7, and check Ch) were evaluated for
grain yield in the multi-environment trial including five locations in Serbia during 2020.
and 2021. year. Stability analyses included twenty parametric and non-parametric indices,
Principal Component Analysis (PCA) and Pearson’s correlations between stability
indices. The sum of ranks for tested late maturity maize hybrids were in the interval from
37 (ZP6) to 146 (ZP4). The average rank was in the range from 1.8 (ZP6) to 7.3 (ZP4).
The interval of variation for standard deviation of average ranks for tested maize hybrids
was from 1.3 (ZP4, Ch) to 2 (ZP2) indicating satisfying accordance of utilized parametric
and non-parametric stability measures. The highest average grain yield across all tested
environments was observed for ZP1, which was second most stable hybrid. The most
stable late maturity maize hybrid tested over ten environments was proven to be ZP6 with
sum of ranks and average rank of 37 and 1.8, respectively, with standard deviation of
average rank 1.5. PCA biplot showed two groups of parametric and non-parametric stability estimates: I group-coefficient of variation (CVi), Eberhart and Russel’s regression coefficient (bi), Perkins and Jinks’s regression coefficient (Bi), GEI variance component (θ(i)), coefficient of determination ( ); II group-Eberhart and Russel’s deviation from regression , Wricke’s ecovalence Shukla’s stability variance Perkins and Jink’s deviation from regression , superiority measure the mean of the absolute rank differences of a genotype over all tested environments the variance among the genotype ranks over all tested environments (the sum of the absolute deviations for each genotype relative to the mean of ranks , the sum of squares of rank for each genotype relative to the mean of ranks - Thennarasu non-parametric stability estimates, mean variance component (θi), Kang’s rank-sum (KRi). Pearson’s correlation bring along the redundant stability indices and one can choose to use or bi or Bi, or θi, or θi.
PB  - Beograd : Društvo genetičara Srbije
T2  - Genetika
T1  - Grain yield stability estimates of late maturity maize hybrids
VL  - 55
IS  - 2
SP  - 505
EP  - 522
DO  - 10.2298/GENSR2302505P
ER  - 
@article{
author = "Pavlov, Jovan and Delić, Nenad and Čamdžija, Zoran and Branković, Gordana and Milosavljević ex Glišović, Nataša and Grčić, Nikola and Božinović, Sofija",
year = "2023",
abstract = "With global climate change including unpredictable geographic and temporal weather
patterns that lead to significant genotype × environment interaction (GEI) the maize
performance assessment would need to be complemented with stability analysis. The
objectives of this study were: i) estimation of parametric and non-parametric stability
indices for grain yield of late maturity maize hybrids, ii) assessing correlations and
grouping of stability indices for grain yield of late maturity maize hybrids. The eight
maize hybrids of FAO 700 maturity group (ZP1-ZP7, and check Ch) were evaluated for
grain yield in the multi-environment trial including five locations in Serbia during 2020.
and 2021. year. Stability analyses included twenty parametric and non-parametric indices,
Principal Component Analysis (PCA) and Pearson’s correlations between stability
indices. The sum of ranks for tested late maturity maize hybrids were in the interval from
37 (ZP6) to 146 (ZP4). The average rank was in the range from 1.8 (ZP6) to 7.3 (ZP4).
The interval of variation for standard deviation of average ranks for tested maize hybrids
was from 1.3 (ZP4, Ch) to 2 (ZP2) indicating satisfying accordance of utilized parametric
and non-parametric stability measures. The highest average grain yield across all tested
environments was observed for ZP1, which was second most stable hybrid. The most
stable late maturity maize hybrid tested over ten environments was proven to be ZP6 with
sum of ranks and average rank of 37 and 1.8, respectively, with standard deviation of
average rank 1.5. PCA biplot showed two groups of parametric and non-parametric stability estimates: I group-coefficient of variation (CVi), Eberhart and Russel’s regression coefficient (bi), Perkins and Jinks’s regression coefficient (Bi), GEI variance component (θ(i)), coefficient of determination ( ); II group-Eberhart and Russel’s deviation from regression , Wricke’s ecovalence Shukla’s stability variance Perkins and Jink’s deviation from regression , superiority measure the mean of the absolute rank differences of a genotype over all tested environments the variance among the genotype ranks over all tested environments (the sum of the absolute deviations for each genotype relative to the mean of ranks , the sum of squares of rank for each genotype relative to the mean of ranks - Thennarasu non-parametric stability estimates, mean variance component (θi), Kang’s rank-sum (KRi). Pearson’s correlation bring along the redundant stability indices and one can choose to use or bi or Bi, or θi, or θi.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "Genetika",
title = "Grain yield stability estimates of late maturity maize hybrids",
volume = "55",
number = "2",
pages = "505-522",
doi = "10.2298/GENSR2302505P"
}
Pavlov, J., Delić, N., Čamdžija, Z., Branković, G., Milosavljević ex Glišović, N., Grčić, N.,& Božinović, S.. (2023). Grain yield stability estimates of late maturity maize hybrids. in Genetika
Beograd : Društvo genetičara Srbije., 55(2), 505-522.
https://doi.org/10.2298/GENSR2302505P
Pavlov J, Delić N, Čamdžija Z, Branković G, Milosavljević ex Glišović N, Grčić N, Božinović S. Grain yield stability estimates of late maturity maize hybrids. in Genetika. 2023;55(2):505-522.
doi:10.2298/GENSR2302505P .
Pavlov, Jovan, Delić, Nenad, Čamdžija, Zoran, Branković, Gordana, Milosavljević ex Glišović, Nataša, Grčić, Nikola, Božinović, Sofija, "Grain yield stability estimates of late maturity maize hybrids" in Genetika, 55, no. 2 (2023):505-522,
https://doi.org/10.2298/GENSR2302505P . .

Current trends in maize breeding at Maize Research Institute “Zemun Polje”

Pavlov, Jovan; Delić, Nenad; Božinović, Sofija; Čamdžija, Zoran; Grčić, Nikola

(Banja Luka : University of Banja Luka, Faculty of Agriculture, 2023)

TY  - GEN
AU  - Pavlov, Jovan
AU  - Delić, Nenad
AU  - Božinović, Sofija
AU  - Čamdžija, Zoran
AU  - Grčić, Nikola
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1348
AB  - Maize breeding program at the Maize Research Institute "Zemun Polje" begun
during the fifties of the last century and it was based on the principles of
inbreeding hybridization. During the sixties, first maize hybrids were
registered and each subsequent decade a new generation of hybrids was
created. So far, more than 800 maize hybrids have been registered in Serbia
and more than 170 hybrids abroad. Testing program includes field evaluations
of experimental hybrids in a large number of locations in Serbia, paying
attention to the correct choice of environments. Each season 5000-7000 new
hybrid combinations have been tested in Serbia. Additionally, the most
promising hybrids of different maturity groups are being tested at more than
20 locations abroad, with the aim of selecting the best hybrids for each foreign
market. In order to accelerate breeding procedure, service of winter generation
in Chile is used, with the aim of obtaining two generations during one calendar
year. Since 2014, the application of double haploid technology in maize
breeding has started. So far, 13000 double haploid lines have been created from
350 original populations. Optimization of each step in the methodology
towards the higher efficiency is the main task for breeders: higher induction
rate, higher duplication rate, better agronomic traits of inducers. This year the
first hybrid containing a double haploid line has been registered. In addition,
several hybrids developed using this method are in registration procedure.
Application of molecular markers represents an essential part of modern
breeding programs. Use of SNP markers became a routine tool in breeding
program of Maize Research Institute. In the last few years, 400 most elite ZP
maize inbred lines were characterized using this type of markers. In addition
to the creation of hybrids with standard grain quality, breeding program of
specific maize hybrids exists and it includes breeding for hybrids with white
endosperm, sweet maize and popcorn.
Breeding for herbicide tolerance resulted in registration of several cycloxidim
tolerant hybrids during last decade. Also, several hybrids with red grain color,
46
which are characterized by higher anthocyanin content, have been registered
so far. Recent innovation in maize breeding program is implementation of
cloud-based software platform, which will enable better data collection and
management, creation of new experiements and improved statistical analysis.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
T2  - 12. international symposium on agricultural sciences „AgroRes 2023“, Trebinje, 24– 26.05.2023. - Book of abstracts
T1  - Current trends in maize breeding at Maize Research Institute “Zemun Polje”
SP  - 45
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_rik_1348
ER  - 
@misc{
author = "Pavlov, Jovan and Delić, Nenad and Božinović, Sofija and Čamdžija, Zoran and Grčić, Nikola",
year = "2023",
abstract = "Maize breeding program at the Maize Research Institute "Zemun Polje" begun
during the fifties of the last century and it was based on the principles of
inbreeding hybridization. During the sixties, first maize hybrids were
registered and each subsequent decade a new generation of hybrids was
created. So far, more than 800 maize hybrids have been registered in Serbia
and more than 170 hybrids abroad. Testing program includes field evaluations
of experimental hybrids in a large number of locations in Serbia, paying
attention to the correct choice of environments. Each season 5000-7000 new
hybrid combinations have been tested in Serbia. Additionally, the most
promising hybrids of different maturity groups are being tested at more than
20 locations abroad, with the aim of selecting the best hybrids for each foreign
market. In order to accelerate breeding procedure, service of winter generation
in Chile is used, with the aim of obtaining two generations during one calendar
year. Since 2014, the application of double haploid technology in maize
breeding has started. So far, 13000 double haploid lines have been created from
350 original populations. Optimization of each step in the methodology
towards the higher efficiency is the main task for breeders: higher induction
rate, higher duplication rate, better agronomic traits of inducers. This year the
first hybrid containing a double haploid line has been registered. In addition,
several hybrids developed using this method are in registration procedure.
Application of molecular markers represents an essential part of modern
breeding programs. Use of SNP markers became a routine tool in breeding
program of Maize Research Institute. In the last few years, 400 most elite ZP
maize inbred lines were characterized using this type of markers. In addition
to the creation of hybrids with standard grain quality, breeding program of
specific maize hybrids exists and it includes breeding for hybrids with white
endosperm, sweet maize and popcorn.
Breeding for herbicide tolerance resulted in registration of several cycloxidim
tolerant hybrids during last decade. Also, several hybrids with red grain color,
46
which are characterized by higher anthocyanin content, have been registered
so far. Recent innovation in maize breeding program is implementation of
cloud-based software platform, which will enable better data collection and
management, creation of new experiements and improved statistical analysis.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "12. international symposium on agricultural sciences „AgroRes 2023“, Trebinje, 24– 26.05.2023. - Book of abstracts",
title = "Current trends in maize breeding at Maize Research Institute “Zemun Polje”",
pages = "45-46",
url = "https://hdl.handle.net/21.15107/rcub_rik_1348"
}
Pavlov, J., Delić, N., Božinović, S., Čamdžija, Z.,& Grčić, N.. (2023). Current trends in maize breeding at Maize Research Institute “Zemun Polje”. in 12. international symposium on agricultural sciences „AgroRes 2023“, Trebinje, 24– 26.05.2023. - Book of abstracts
Banja Luka : University of Banja Luka, Faculty of Agriculture., 45-46.
https://hdl.handle.net/21.15107/rcub_rik_1348
Pavlov J, Delić N, Božinović S, Čamdžija Z, Grčić N. Current trends in maize breeding at Maize Research Institute “Zemun Polje”. in 12. international symposium on agricultural sciences „AgroRes 2023“, Trebinje, 24– 26.05.2023. - Book of abstracts. 2023;:45-46.
https://hdl.handle.net/21.15107/rcub_rik_1348 .
Pavlov, Jovan, Delić, Nenad, Božinović, Sofija, Čamdžija, Zoran, Grčić, Nikola, "Current trends in maize breeding at Maize Research Institute “Zemun Polje”" in 12. international symposium on agricultural sciences „AgroRes 2023“, Trebinje, 24– 26.05.2023. - Book of abstracts (2023):45-46,
https://hdl.handle.net/21.15107/rcub_rik_1348 .

ZP 5557- Novi hibrid kukuruza

Pavlov, Jovan; Delić, Nenad

(Beograd : Društvo genetičara Srbije, 2023)

TY  - CONF
AU  - Pavlov, Jovan
AU  - Delić, Nenad
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1346
AB  - Hibridi srednje grupe zrenja FAO 500 imaju sve veći značaj u proizvodnji kukuruza
u Srbiji. Imajući u vidu da je u poslednjih nekoliko godina prisutan trend
gajenja hibrida koji imaju niži sadržaj vlage u momentu žetve, hibridi grupe zrenja
FAO 500 predstavljaju odličnu kombinaciju visokog prinosa i nižeg sadržaja vlage
u zrnu. Mogu se ubirati i u klipu i u zrnu, što ih čini veoma dobrom alternativom
za hibride kasnih grupa zrenja koji se odlikuju visokim potencijalom za prinos
ali i visokim sadržajem vlage u žetvi. Hibrid ZP 5557 registrovan je 2023. godine.
U ogledima Sortne komisije je ispitivan u periodu 2021-2022. godine. Karakteristično
je da su obe godine bile nepovoljne za gajenje kukuruza, što se naročito
odnosi na 2022. godinu. Hibrid ZP 5557 je u obe godine ispitivanja ostvario viši
prosečan prinos u odnosu na prosek standardnih hibrida, uz niži sadržaj vlage i
manji procenat poleglih i slomljenih biljaka. U dvogodišnjem proseku ostvario je
prinos od 104,4% u odnosu na prosek standardnih hibrida. Na osnovu rezultata
postignutih u ogledima sortne komisije, hibrid ZP 5557 biće uključen u postkomisijske
oglede Instituta za kukuruz i u slučaju da ponovi dobar rezultat biće
započeta komercijalizacija ovog hibrida.
AB  - Hybrids of medium maturity group FAO 500 become more important in maize
production in Serbia. Taking into account that in the last few years there has been
a trend of growing hybrids with lower harvest moisture, the hybrids of the FAO
500 maturity group represent an excellent combination of high yield and lower
grain moisture content in harvest. They can be harvested both in ear and grain,
which makes them a very good alternative for hybrids of late maturity groups
that are characterized by high yield potential but also high moisture content at
harvest. Hybrid ZP 5557 was registered in 2023. It was tested in the trials of the
State Variety Commission in the period 2021-2022 years. It is characteristic that
both years were unfavorable for growing maize, which especially applies to the
year 2022. Hybrid ZP 5557 achieved a higher average yield in both years of testing
compared to the average of check hybrids, with lower moisture content and a
lower percentage of broken and lodged plants. In the two-year average, it achieved
a yield of 104.4% compared to the average of check hybrids. Based on the results
achieved in the tests of the variety commission, hybrid ZP 5557 will be included
in the post-official trials of the Maize Research Institute and in case it obtain good
result, the commercialization of this hybrid will be started.
PB  - Beograd : Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
C3  - 10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta
T1  - ZP 5557- Novi hibrid kukuruza
T1  - ZP 5557 – New maize hybrid
SP  - 225
EP  - 226
UR  - https://hdl.handle.net/21.15107/rcub_rik_1346
ER  - 
@conference{
author = "Pavlov, Jovan and Delić, Nenad",
year = "2023",
abstract = "Hibridi srednje grupe zrenja FAO 500 imaju sve veći značaj u proizvodnji kukuruza
u Srbiji. Imajući u vidu da je u poslednjih nekoliko godina prisutan trend
gajenja hibrida koji imaju niži sadržaj vlage u momentu žetve, hibridi grupe zrenja
FAO 500 predstavljaju odličnu kombinaciju visokog prinosa i nižeg sadržaja vlage
u zrnu. Mogu se ubirati i u klipu i u zrnu, što ih čini veoma dobrom alternativom
za hibride kasnih grupa zrenja koji se odlikuju visokim potencijalom za prinos
ali i visokim sadržajem vlage u žetvi. Hibrid ZP 5557 registrovan je 2023. godine.
U ogledima Sortne komisije je ispitivan u periodu 2021-2022. godine. Karakteristično
je da su obe godine bile nepovoljne za gajenje kukuruza, što se naročito
odnosi na 2022. godinu. Hibrid ZP 5557 je u obe godine ispitivanja ostvario viši
prosečan prinos u odnosu na prosek standardnih hibrida, uz niži sadržaj vlage i
manji procenat poleglih i slomljenih biljaka. U dvogodišnjem proseku ostvario je
prinos od 104,4% u odnosu na prosek standardnih hibrida. Na osnovu rezultata
postignutih u ogledima sortne komisije, hibrid ZP 5557 biće uključen u postkomisijske
oglede Instituta za kukuruz i u slučaju da ponovi dobar rezultat biće
započeta komercijalizacija ovog hibrida., Hybrids of medium maturity group FAO 500 become more important in maize
production in Serbia. Taking into account that in the last few years there has been
a trend of growing hybrids with lower harvest moisture, the hybrids of the FAO
500 maturity group represent an excellent combination of high yield and lower
grain moisture content in harvest. They can be harvested both in ear and grain,
which makes them a very good alternative for hybrids of late maturity groups
that are characterized by high yield potential but also high moisture content at
harvest. Hybrid ZP 5557 was registered in 2023. It was tested in the trials of the
State Variety Commission in the period 2021-2022 years. It is characteristic that
both years were unfavorable for growing maize, which especially applies to the
year 2022. Hybrid ZP 5557 achieved a higher average yield in both years of testing
compared to the average of check hybrids, with lower moisture content and a
lower percentage of broken and lodged plants. In the two-year average, it achieved
a yield of 104.4% compared to the average of check hybrids. Based on the results
achieved in the tests of the variety commission, hybrid ZP 5557 will be included
in the post-official trials of the Maize Research Institute and in case it obtain good
result, the commercialization of this hybrid will be started.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta",
title = "ZP 5557- Novi hibrid kukuruza, ZP 5557 – New maize hybrid",
pages = "225-226",
url = "https://hdl.handle.net/21.15107/rcub_rik_1346"
}
Pavlov, J.,& Delić, N.. (2023). ZP 5557- Novi hibrid kukuruza. in 10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta
Beograd : Društvo genetičara Srbije., 225-226.
https://hdl.handle.net/21.15107/rcub_rik_1346
Pavlov J, Delić N. ZP 5557- Novi hibrid kukuruza. in 10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta. 2023;:225-226.
https://hdl.handle.net/21.15107/rcub_rik_1346 .
Pavlov, Jovan, Delić, Nenad, "ZP 5557- Novi hibrid kukuruza" in 10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta (2023):225-226,
https://hdl.handle.net/21.15107/rcub_rik_1346 .

Primena novih tehnologija u oplemenjivanju kukuruza u Institutu za kukuruz „Zemun Polje"

Čamdžija, Zoran; Filipović, Milomir; Delić, Nenad; Pavlov, Jovan; Božinović, Sofija; Sečanski, Mile; Mladenović, Marko

(Beograd : Društvo genetičara Srbije, 2023)

TY  - CONF
AU  - Čamdžija, Zoran
AU  - Filipović, Milomir
AU  - Delić, Nenad
AU  - Pavlov, Jovan
AU  - Božinović, Sofija
AU  - Sečanski, Mile
AU  - Mladenović, Marko
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1277
AB  - Institut za kukuruz "Zemun Polje" (MRIZP) razvija program oplemenjivanja kukuruza vise od 75 godina. Stvaranje novih hibrida, koji po svojim najvažnijim agronomskim osobinama prevazilaze hibride prethodne generacije predstavlja primarni zadatak svakog oplemenjivača kukuruza. Prisustvo ZP hibrida kukuruza na tržištima Republike Srbije, ali i u inostranstvu, zahteva stvaranje hibrida za različite agroklimatske uslove, kao i za razlicite namene. Spajanje različitih naučno istraživačkih disciplina dovelo je do ubrzanja i povećanja tačnosti procesa oplemenjivanja kukuruza, a samim tim i do smanjenja trajanja ciklusa oplemenjivanja kukuruza u svetu do pet, a u MRIZP-u na 6-7 godina. Od 2014. godine MRIZP uspešno primenjuje program dvostrukih haploida (DH) u oplemenjivanju. Primenom takvog pristupa storeno je više od 13.000 novih DH inbred linija. Kao rezultat primene DH tehnologije, registrovan je prvi hibrid ZP 4019. MRIZP intenzivno radi na karakterizaciji najelitnijeg materijala uz pomoc SNP (Single Nucleotide Markers) markera koristeći 25k SNP Ilumina Infinium Array. Na ovaj način je ostvaren najprecizniji uvid u germplazmu oplemenjivača, odnosno odredjivanjem genetičke udaljenosti (GD). Uz pomoć GD vrednosti povećana je verovatnoća dobijanja superiornih inbred linija i hibrida kukuruza. Već dve sezone MRIZP je započeo visokoinformativnu fenotipizaciju u polju (HTFP), koristeći RGB i multispektralne kamere zasnovane na dronovima za ekstrakciju i vizuelizaciju podataka po parceli. U ovoi fazi istražujemo mogućnost zamene ručnih merenja (kao što je broj i poleganje biljaka), dok je naš konačni cilj da upotrebimo HTFP za donošenje presudnih odluka za hibrid u našim programima oplemenjivanja.
PB  - Beograd : Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
C3  - X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta
T1  - Primena novih tehnologija u oplemenjivanju kukuruza u Institutu za kukuruz „Zemun Polje"
SP  - 31
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_rik_1277
ER  - 
@conference{
author = "Čamdžija, Zoran and Filipović, Milomir and Delić, Nenad and Pavlov, Jovan and Božinović, Sofija and Sečanski, Mile and Mladenović, Marko",
year = "2023",
abstract = "Institut za kukuruz "Zemun Polje" (MRIZP) razvija program oplemenjivanja kukuruza vise od 75 godina. Stvaranje novih hibrida, koji po svojim najvažnijim agronomskim osobinama prevazilaze hibride prethodne generacije predstavlja primarni zadatak svakog oplemenjivača kukuruza. Prisustvo ZP hibrida kukuruza na tržištima Republike Srbije, ali i u inostranstvu, zahteva stvaranje hibrida za različite agroklimatske uslove, kao i za razlicite namene. Spajanje različitih naučno istraživačkih disciplina dovelo je do ubrzanja i povećanja tačnosti procesa oplemenjivanja kukuruza, a samim tim i do smanjenja trajanja ciklusa oplemenjivanja kukuruza u svetu do pet, a u MRIZP-u na 6-7 godina. Od 2014. godine MRIZP uspešno primenjuje program dvostrukih haploida (DH) u oplemenjivanju. Primenom takvog pristupa storeno je više od 13.000 novih DH inbred linija. Kao rezultat primene DH tehnologije, registrovan je prvi hibrid ZP 4019. MRIZP intenzivno radi na karakterizaciji najelitnijeg materijala uz pomoc SNP (Single Nucleotide Markers) markera koristeći 25k SNP Ilumina Infinium Array. Na ovaj način je ostvaren najprecizniji uvid u germplazmu oplemenjivača, odnosno odredjivanjem genetičke udaljenosti (GD). Uz pomoć GD vrednosti povećana je verovatnoća dobijanja superiornih inbred linija i hibrida kukuruza. Već dve sezone MRIZP je započeo visokoinformativnu fenotipizaciju u polju (HTFP), koristeći RGB i multispektralne kamere zasnovane na dronovima za ekstrakciju i vizuelizaciju podataka po parceli. U ovoi fazi istražujemo mogućnost zamene ručnih merenja (kao što je broj i poleganje biljaka), dok je naš konačni cilj da upotrebimo HTFP za donošenje presudnih odluka za hibrid u našim programima oplemenjivanja.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta",
title = "Primena novih tehnologija u oplemenjivanju kukuruza u Institutu za kukuruz „Zemun Polje"",
pages = "31-31",
url = "https://hdl.handle.net/21.15107/rcub_rik_1277"
}
Čamdžija, Z., Filipović, M., Delić, N., Pavlov, J., Božinović, S., Sečanski, M.,& Mladenović, M.. (2023). Primena novih tehnologija u oplemenjivanju kukuruza u Institutu za kukuruz „Zemun Polje". in X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta
Beograd : Društvo genetičara Srbije., 31-31.
https://hdl.handle.net/21.15107/rcub_rik_1277
Čamdžija Z, Filipović M, Delić N, Pavlov J, Božinović S, Sečanski M, Mladenović M. Primena novih tehnologija u oplemenjivanju kukuruza u Institutu za kukuruz „Zemun Polje". in X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta. 2023;:31-31.
https://hdl.handle.net/21.15107/rcub_rik_1277 .
Čamdžija, Zoran, Filipović, Milomir, Delić, Nenad, Pavlov, Jovan, Božinović, Sofija, Sečanski, Mile, Mladenović, Marko, "Primena novih tehnologija u oplemenjivanju kukuruza u Institutu za kukuruz „Zemun Polje"" in X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta (2023):31-31,
https://hdl.handle.net/21.15107/rcub_rik_1277 .

ZP 5557

Pavlov, Jovan; Delić, Nenad; Todorović, Goran

(Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja, 2023)

TY  - PAT
AU  - Pavlov, Jovan
AU  - Delić, Nenad
AU  - Todorović, Goran
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1271
PB  - Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja
T2  - Rešenje broj 320-09-02462/2/2021-11 od 23.03.2023. godine
T1  - ZP 5557
UR  - https://hdl.handle.net/21.15107/rcub_rik_1271
ER  - 
@misc{
author = "Pavlov, Jovan and Delić, Nenad and Todorović, Goran",
year = "2023",
publisher = "Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja",
journal = "Rešenje broj 320-09-02462/2/2021-11 od 23.03.2023. godine",
title = "ZP 5557",
url = "https://hdl.handle.net/21.15107/rcub_rik_1271"
}
Pavlov, J., Delić, N.,& Todorović, G.. (2023). ZP 5557. in Rešenje broj 320-09-02462/2/2021-11 od 23.03.2023. godine
Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja..
https://hdl.handle.net/21.15107/rcub_rik_1271
Pavlov J, Delić N, Todorović G. ZP 5557. in Rešenje broj 320-09-02462/2/2021-11 od 23.03.2023. godine. 2023;.
https://hdl.handle.net/21.15107/rcub_rik_1271 .
Pavlov, Jovan, Delić, Nenad, Todorović, Goran, "ZP 5557" in Rešenje broj 320-09-02462/2/2021-11 od 23.03.2023. godine (2023),
https://hdl.handle.net/21.15107/rcub_rik_1271 .

ZP 5255

Stevanović, Milan; Delić, Nenad; Nikolić, Ana; Filipović, Milomir

(Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja, 2023)

TY  - PAT
AU  - Stevanović, Milan
AU  - Delić, Nenad
AU  - Nikolić, Ana
AU  - Filipović, Milomir
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1250
PB  - Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja
T2  - Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine
T1  - ZP 5255
UR  - https://hdl.handle.net/21.15107/rcub_rik_1250
ER  - 
@misc{
author = "Stevanović, Milan and Delić, Nenad and Nikolić, Ana and Filipović, Milomir",
year = "2023",
publisher = "Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja",
journal = "Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine",
title = "ZP 5255",
url = "https://hdl.handle.net/21.15107/rcub_rik_1250"
}
Stevanović, M., Delić, N., Nikolić, A.,& Filipović, M.. (2023). ZP 5255. in Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine
Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja..
https://hdl.handle.net/21.15107/rcub_rik_1250
Stevanović M, Delić N, Nikolić A, Filipović M. ZP 5255. in Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine. 2023;.
https://hdl.handle.net/21.15107/rcub_rik_1250 .
Stevanović, Milan, Delić, Nenad, Nikolić, Ana, Filipović, Milomir, "ZP 5255" in Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine (2023),
https://hdl.handle.net/21.15107/rcub_rik_1250 .

ZP 4542

Delić, Nenad; Pavlov, Jovan; Filipović, Milomir

(Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja, 2023)

TY  - PAT
AU  - Delić, Nenad
AU  - Pavlov, Jovan
AU  - Filipović, Milomir
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1245
PB  - Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja
T2  - Rešenje broj 320-09-02449/2/2021-11 od 30.03.2023. godine
T1  - ZP 4542
UR  - https://hdl.handle.net/21.15107/rcub_rik_1245
ER  - 
@misc{
author = "Delić, Nenad and Pavlov, Jovan and Filipović, Milomir",
year = "2023",
publisher = "Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja",
journal = "Rešenje broj 320-09-02449/2/2021-11 od 30.03.2023. godine",
title = "ZP 4542",
url = "https://hdl.handle.net/21.15107/rcub_rik_1245"
}
Delić, N., Pavlov, J.,& Filipović, M.. (2023). ZP 4542. in Rešenje broj 320-09-02449/2/2021-11 od 30.03.2023. godine
Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja..
https://hdl.handle.net/21.15107/rcub_rik_1245
Delić N, Pavlov J, Filipović M. ZP 4542. in Rešenje broj 320-09-02449/2/2021-11 od 30.03.2023. godine. 2023;.
https://hdl.handle.net/21.15107/rcub_rik_1245 .
Delić, Nenad, Pavlov, Jovan, Filipović, Milomir, "ZP 4542" in Rešenje broj 320-09-02449/2/2021-11 od 30.03.2023. godine (2023),
https://hdl.handle.net/21.15107/rcub_rik_1245 .

Adapted Quality Protein Maize in Broiler Diets Holds Nutritive and Financial Advantages

Kostadinović, Marija; Vančetović, Jelena; Delić, Nenad; Ignjatović-Micić, Dragana

(Beograd: Institut za stočarstvo, 2023)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Ignjatović-Micić, Dragana
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1187
AB  - Quality protein maize (QPM) can be beneficial for use in feeds as it was shown that substitution of standard maize (SM) with QPM can improve livestock performance and decrease costly dietary lysine supplementation and protein ingredients. Herein, nutritional and financial effects of QPM adapted to temperate climate in broiler diets were tested. Feeding experiment was performed on two groups of broilers – control (fed with SM) and treatment (fed with QPM). Duration of each feeding trial was 42 days, comprising three phases - starter (1-14), grower (15-35) and finisher (36-42). Diets for all growth phases were formulated based on the biochemical analysis of SM and QPM kernels. Body weight of the chickens was measured on days 1, 7, 14, 35 and 42. Feed intake (FI), body weight gain (BWG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. Two trials were performed. In the first, SM was completely replaced with QPM in treatment group diets. As the results indicated better FCR in the treatment group for grower (1.49:1.54) and finisher (1.55:1.60) phases, in the second trial QPM was increased and soybean component decreased by 3%. That led to better results as FCR was even lower but in all three phases, confirming the results of the first trial and further confirming the effects of QPM on feed conversion. Moreover, significant financial reduction in feed was observed, as the price of soybean is usually two to four times higher than the price of maize.
PB  - Beograd: Institut za stočarstvo
C3  - 14. international symposium "Modern trends in livestock production", 4-6.10.2023., Belgrade, Serbia - Book of abstracts
T1  - Adapted Quality Protein Maize in Broiler Diets Holds Nutritive and Financial Advantages
UR  - https://hdl.handle.net/21.15107/rcub_rik_1187
ER  - 
@conference{
author = "Kostadinović, Marija and Vančetović, Jelena and Delić, Nenad and Ignjatović-Micić, Dragana",
year = "2023",
abstract = "Quality protein maize (QPM) can be beneficial for use in feeds as it was shown that substitution of standard maize (SM) with QPM can improve livestock performance and decrease costly dietary lysine supplementation and protein ingredients. Herein, nutritional and financial effects of QPM adapted to temperate climate in broiler diets were tested. Feeding experiment was performed on two groups of broilers – control (fed with SM) and treatment (fed with QPM). Duration of each feeding trial was 42 days, comprising three phases - starter (1-14), grower (15-35) and finisher (36-42). Diets for all growth phases were formulated based on the biochemical analysis of SM and QPM kernels. Body weight of the chickens was measured on days 1, 7, 14, 35 and 42. Feed intake (FI), body weight gain (BWG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. Two trials were performed. In the first, SM was completely replaced with QPM in treatment group diets. As the results indicated better FCR in the treatment group for grower (1.49:1.54) and finisher (1.55:1.60) phases, in the second trial QPM was increased and soybean component decreased by 3%. That led to better results as FCR was even lower but in all three phases, confirming the results of the first trial and further confirming the effects of QPM on feed conversion. Moreover, significant financial reduction in feed was observed, as the price of soybean is usually two to four times higher than the price of maize.",
publisher = "Beograd: Institut za stočarstvo",
journal = "14. international symposium "Modern trends in livestock production", 4-6.10.2023., Belgrade, Serbia - Book of abstracts",
title = "Adapted Quality Protein Maize in Broiler Diets Holds Nutritive and Financial Advantages",
url = "https://hdl.handle.net/21.15107/rcub_rik_1187"
}
Kostadinović, M., Vančetović, J., Delić, N.,& Ignjatović-Micić, D.. (2023). Adapted Quality Protein Maize in Broiler Diets Holds Nutritive and Financial Advantages. in 14. international symposium "Modern trends in livestock production", 4-6.10.2023., Belgrade, Serbia - Book of abstracts
Beograd: Institut za stočarstvo..
https://hdl.handle.net/21.15107/rcub_rik_1187
Kostadinović M, Vančetović J, Delić N, Ignjatović-Micić D. Adapted Quality Protein Maize in Broiler Diets Holds Nutritive and Financial Advantages. in 14. international symposium "Modern trends in livestock production", 4-6.10.2023., Belgrade, Serbia - Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_rik_1187 .
Kostadinović, Marija, Vančetović, Jelena, Delić, Nenad, Ignjatović-Micić, Dragana, "Adapted Quality Protein Maize in Broiler Diets Holds Nutritive and Financial Advantages" in 14. international symposium "Modern trends in livestock production", 4-6.10.2023., Belgrade, Serbia - Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_rik_1187 .

ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.)

Radenovic, Cedomir; Maksimov, Georgy; Kuramshina, Gulnara; Shutova, Vitalina; Hao, Jingrao; Delić, Nenad; Sečanski, Mile; Popović, Aleksandar; Bajuk-Bogdanovic, Danica; Radosavljević, Milica; Pavlov, Jovan

(Moskva : Сельскохозяйственная биология, 2022)

TY  - JOUR
AU  - Radenovic, Cedomir
AU  - Maksimov, Georgy
AU  - Kuramshina, Gulnara
AU  - Shutova, Vitalina
AU  - Hao, Jingrao
AU  - Delić, Nenad
AU  - Sečanski, Mile
AU  - Popović, Aleksandar
AU  - Bajuk-Bogdanovic, Danica
AU  - Radosavljević, Milica
AU  - Pavlov, Jovan
PY  - 2022
UR  - http://agrobiology.ru/5-2022radenovich-eng.html
UR  - http://rik.mrizp.rs/handle/123456789/1354
AB  - Инфракрасная (ИК) спектроскопия и спектроскопия комбинационного рассеяния (КР) — современные методы, на основе которых успешно формируются биотехнологические подходы, поз-воляющие проводить генетический и функциональный анализ отдельных органов и тканей растений на молекулярном уровне. В представленной работе с помощью спектроскопии поверхностного внут-реннего отражения (ПВО), которая представляет собой модификацию ИК-спектроскопии, впервые зарегистрированы и выявлены различия в содержании и конформации биомолекул в гомогенатах целого зерна гибридов кукурузы ZP 735 и его компонентов (эндосперм, перикарп, зародыш). Нашей целью была разработка методологии спектроскопии поверхностного внутреннего отражения для идентификации органических молекул, их содержания и конформации в семени кукурузы и его тканях. Объектом исследования служили зерна гибрида кукурузы (Zea mays L.) ZP 735 (оригина-тор — Maize Research Institute, Zemun Polje, г. Белград, Сербия). Методом случайной выборки было отобрано 30 зерен. Для получения ПВО-спектров образцы целых зерен и их частей (эндо-сперма, перикарпа и зародыша) измельчали, гомогенизировали и помещали в специальную ячейку прибора. Для регистрации ПВО-спектров использовали спектрометр (Nicolet™ iS20 FTIR Spectro-meter, «Thermo Scientific», США) с алмазом (diamond ATR crystal, «Thermo Scientific», США), для анализа спектров — пакет программ («Thermo Scientific», США). ПВО-спектры сопоставляли в библиотеке данных программы Advanced ATR correction Algorithm. Метод ПВО-спектроскопии ос-нован на отражении пучка света на границе раздела двух фаз: фазы кристалла с высоким показа-телем преломления, входящего в состав прибора ПВО, и фазы исследуемого образца с более низ-ким показателем преломления. В процессе измерения луч света проникает на небольшую глубину в фазу образца, где частично поглощается. При последующих облучениях образца это явление повторяется, и в результате регистрируется ПВО-спектр. Доказано, что в диапазоне от 400 см1 до 4000 см1 ПВО-спектры целого зерна, эндосперма, перикарпа и зародыша гибрида ZP 735 были аналогичны полученным ранее ИК-спектрам зерна, что свидетельствует о возможности использо-вания нового метода для технологий молекулярной селекции. Расположение полос ПВО-спектра характеризует различные формы колебаний валентных связей функциональных групп органических молекул семени, что позволяет идентифицировать не только наличие определенных молекул, но и их конформацию. Доказано, что с помощью анализа амплитуды полос ПВО-спектра (максималь-ная амплитуда интенсивности и высокая амплитуда интенсивности) в тканях семени можно кон-тролировать изменение содержания ряда органических соединений: протеинов, липидов, сахаров, сложных эфиров, амидов, кетонов, альдегидов, карбоновых кислот, простых эфиров, фенолов, спиртов, ароматических углеводородов, ациклических соединений, алкенов, алканов и алкинов. Полученные результаты важны для тестирования наличия в зерне необходимых органических со-единений или изменения их конформации в процессе селекции. К важным преимуществам метода ПВО-пектроскопии по сравнению с ИК-спектроскопией относятся, с одной стороны, сохранение нативности объекта (возможность проведения исследования без фиксации или окрашивания) и простая пробоподготовка, с другой — эффективная оценка содержания и конформации молекул с высокой чувствительностью (разрешение порядка 1,0 см1). Реализация разработанной методики для формирования технологии молекулярной селекции повысит рентабельность культивирования и эффективность селекции не только кукурузы, но и других сельскохозяйственных растений.
AB  - Infrared (IR) spectroscopy and Raman spectroscopy (RS) are modern methods on the basis of which biotechnological approaches are being successfully developed that allow genetic and func-tional analysis of individual plant organs and tissues at the molecular level. In the present work, using surface internal reflection spectroscopy (SIR), which is a modification of IR spectroscopy, differences in the content and conformation of biomolecules in grain homogenates and its components (endo-sperm, pericarp, germ) of ZP 735 maize hybrids were first recorded and revealed. Our goal was to develop a methodology for surface SIR for the identification of organic molecules, their content and conformation in corn seed and its tissues. The grains of the corn hybrid (Zea mays L.) ZP 735 (origi-nator Maize Research Institute, Zemun Polje, Belgrade, Serbia) served as the object of the study. Thirty grains were selected by random sampling. To obtain SIR spectra, samples of grains and their parts (endosperm, pericarp, and embryo) were crushed, homogenized, and placed in a special cell of the device. A spectrometer (FT-IR spectrophotometer, Thermo Scientific, USA) with diamond (dia-mond ATR crystal, Thermo Scientific, USA) was used to record the SIR spectra, and a software package (Thermo Scientific™ ) was used to analyze the spectra. The SIR spectra were compared in the data library of the Advanced ATR correction Algorithm program. The SIR spectroscopy method is based on the reflection of a light beam at the interface between two phases: the phase of a crystal with a high refractive index, which is part of the SIR device, and the phase of the sample under study with a lower refractive index. During the measurement, the light beam penetrates to a small depth into the phase of the sample, where it is partially absorbed. During subsequent irradiation of the sample, this phenomenon is repeated, and as a result, the SIR spectrum is recorded. It has been proven that in the range from 400 cm-1 to 4000 cm-1 ATR spectra of whole grain, endosperm, pericarp and embryo of the ZP 735 hybrid are similar to the previously obtained IR spectra of grain, which indicates the possibility of using a new method for molecular breeding technologies. The location of the bands of the SIR spectrum characterizes various forms of vibrations of the valence bonds of the functional groups of the organic molecules of the seed, which makes it possible to identify not only the presence of certain molecules, but also their conformation. It has been proved that by analyzing the amplitude of the SIR spectrum bands (maximum intensity amplitude and high intensity amplitude), it is possible to control changes in the content of a number of organic compounds in seed tissues: proteins, lipids, sugars, esters, amides, ketones, aldehydes, carboxylic acids, simple ethers, phenols, alcohols, aromatic hydrocarbons, acyclic compounds, alkenes, alkanes and alkynes. The obtained results are important for testing the presence of the necessary organic compounds in the grain or changes in their confor-mation during the selection process. The important advantages of SIR spectroscopy compared to IR spectroscopy include, on the one hand, the preservation of the nativeness of the object (the possibility of conducting research without fixing or staining the object) and simple sample preparation, on the other hand, an effective assessment of the content and conformation of molecules with high sensitivity (resolution about 1.0 cm-1). The implementation of the developed methodology for the formation of molecular breeding technology will increase the profitability of cultivation and the efficiency of breed-ing not only corn, but also other agricultural plants.
PB  - Moskva : Сельскохозяйственная биология
T2  - Сельскохозяйственная биология [Agricultural Biology]
T1  - ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.)
T1  - USE OF INTERNAL REFLECTION SPECTROSCOPY FOR MAIZE (Zea mays L.) GRAIN DIAGNOSIS
VL  - 57
IS  - 5
SP  - 933
EP  - 944
DO  - 10.15389/agrobiology.2022.5.933rus
ER  - 
@article{
author = "Radenovic, Cedomir and Maksimov, Georgy and Kuramshina, Gulnara and Shutova, Vitalina and Hao, Jingrao and Delić, Nenad and Sečanski, Mile and Popović, Aleksandar and Bajuk-Bogdanovic, Danica and Radosavljević, Milica and Pavlov, Jovan",
year = "2022",
abstract = "Инфракрасная (ИК) спектроскопия и спектроскопия комбинационного рассеяния (КР) — современные методы, на основе которых успешно формируются биотехнологические подходы, поз-воляющие проводить генетический и функциональный анализ отдельных органов и тканей растений на молекулярном уровне. В представленной работе с помощью спектроскопии поверхностного внут-реннего отражения (ПВО), которая представляет собой модификацию ИК-спектроскопии, впервые зарегистрированы и выявлены различия в содержании и конформации биомолекул в гомогенатах целого зерна гибридов кукурузы ZP 735 и его компонентов (эндосперм, перикарп, зародыш). Нашей целью была разработка методологии спектроскопии поверхностного внутреннего отражения для идентификации органических молекул, их содержания и конформации в семени кукурузы и его тканях. Объектом исследования служили зерна гибрида кукурузы (Zea mays L.) ZP 735 (оригина-тор — Maize Research Institute, Zemun Polje, г. Белград, Сербия). Методом случайной выборки было отобрано 30 зерен. Для получения ПВО-спектров образцы целых зерен и их частей (эндо-сперма, перикарпа и зародыша) измельчали, гомогенизировали и помещали в специальную ячейку прибора. Для регистрации ПВО-спектров использовали спектрометр (Nicolet™ iS20 FTIR Spectro-meter, «Thermo Scientific», США) с алмазом (diamond ATR crystal, «Thermo Scientific», США), для анализа спектров — пакет программ («Thermo Scientific», США). ПВО-спектры сопоставляли в библиотеке данных программы Advanced ATR correction Algorithm. Метод ПВО-спектроскопии ос-нован на отражении пучка света на границе раздела двух фаз: фазы кристалла с высоким показа-телем преломления, входящего в состав прибора ПВО, и фазы исследуемого образца с более низ-ким показателем преломления. В процессе измерения луч света проникает на небольшую глубину в фазу образца, где частично поглощается. При последующих облучениях образца это явление повторяется, и в результате регистрируется ПВО-спектр. Доказано, что в диапазоне от 400 см1 до 4000 см1 ПВО-спектры целого зерна, эндосперма, перикарпа и зародыша гибрида ZP 735 были аналогичны полученным ранее ИК-спектрам зерна, что свидетельствует о возможности использо-вания нового метода для технологий молекулярной селекции. Расположение полос ПВО-спектра характеризует различные формы колебаний валентных связей функциональных групп органических молекул семени, что позволяет идентифицировать не только наличие определенных молекул, но и их конформацию. Доказано, что с помощью анализа амплитуды полос ПВО-спектра (максималь-ная амплитуда интенсивности и высокая амплитуда интенсивности) в тканях семени можно кон-тролировать изменение содержания ряда органических соединений: протеинов, липидов, сахаров, сложных эфиров, амидов, кетонов, альдегидов, карбоновых кислот, простых эфиров, фенолов, спиртов, ароматических углеводородов, ациклических соединений, алкенов, алканов и алкинов. Полученные результаты важны для тестирования наличия в зерне необходимых органических со-единений или изменения их конформации в процессе селекции. К важным преимуществам метода ПВО-пектроскопии по сравнению с ИК-спектроскопией относятся, с одной стороны, сохранение нативности объекта (возможность проведения исследования без фиксации или окрашивания) и простая пробоподготовка, с другой — эффективная оценка содержания и конформации молекул с высокой чувствительностью (разрешение порядка 1,0 см1). Реализация разработанной методики для формирования технологии молекулярной селекции повысит рентабельность культивирования и эффективность селекции не только кукурузы, но и других сельскохозяйственных растений., Infrared (IR) spectroscopy and Raman spectroscopy (RS) are modern methods on the basis of which biotechnological approaches are being successfully developed that allow genetic and func-tional analysis of individual plant organs and tissues at the molecular level. In the present work, using surface internal reflection spectroscopy (SIR), which is a modification of IR spectroscopy, differences in the content and conformation of biomolecules in grain homogenates and its components (endo-sperm, pericarp, germ) of ZP 735 maize hybrids were first recorded and revealed. Our goal was to develop a methodology for surface SIR for the identification of organic molecules, their content and conformation in corn seed and its tissues. The grains of the corn hybrid (Zea mays L.) ZP 735 (origi-nator Maize Research Institute, Zemun Polje, Belgrade, Serbia) served as the object of the study. Thirty grains were selected by random sampling. To obtain SIR spectra, samples of grains and their parts (endosperm, pericarp, and embryo) were crushed, homogenized, and placed in a special cell of the device. A spectrometer (FT-IR spectrophotometer, Thermo Scientific, USA) with diamond (dia-mond ATR crystal, Thermo Scientific, USA) was used to record the SIR spectra, and a software package (Thermo Scientific™ ) was used to analyze the spectra. The SIR spectra were compared in the data library of the Advanced ATR correction Algorithm program. The SIR spectroscopy method is based on the reflection of a light beam at the interface between two phases: the phase of a crystal with a high refractive index, which is part of the SIR device, and the phase of the sample under study with a lower refractive index. During the measurement, the light beam penetrates to a small depth into the phase of the sample, where it is partially absorbed. During subsequent irradiation of the sample, this phenomenon is repeated, and as a result, the SIR spectrum is recorded. It has been proven that in the range from 400 cm-1 to 4000 cm-1 ATR spectra of whole grain, endosperm, pericarp and embryo of the ZP 735 hybrid are similar to the previously obtained IR spectra of grain, which indicates the possibility of using a new method for molecular breeding technologies. The location of the bands of the SIR spectrum characterizes various forms of vibrations of the valence bonds of the functional groups of the organic molecules of the seed, which makes it possible to identify not only the presence of certain molecules, but also their conformation. It has been proved that by analyzing the amplitude of the SIR spectrum bands (maximum intensity amplitude and high intensity amplitude), it is possible to control changes in the content of a number of organic compounds in seed tissues: proteins, lipids, sugars, esters, amides, ketones, aldehydes, carboxylic acids, simple ethers, phenols, alcohols, aromatic hydrocarbons, acyclic compounds, alkenes, alkanes and alkynes. The obtained results are important for testing the presence of the necessary organic compounds in the grain or changes in their confor-mation during the selection process. The important advantages of SIR spectroscopy compared to IR spectroscopy include, on the one hand, the preservation of the nativeness of the object (the possibility of conducting research without fixing or staining the object) and simple sample preparation, on the other hand, an effective assessment of the content and conformation of molecules with high sensitivity (resolution about 1.0 cm-1). The implementation of the developed methodology for the formation of molecular breeding technology will increase the profitability of cultivation and the efficiency of breed-ing not only corn, but also other agricultural plants.",
publisher = "Moskva : Сельскохозяйственная биология",
journal = "Сельскохозяйственная биология [Agricultural Biology]",
title = "ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.), USE OF INTERNAL REFLECTION SPECTROSCOPY FOR MAIZE (Zea mays L.) GRAIN DIAGNOSIS",
volume = "57",
number = "5",
pages = "933-944",
doi = "10.15389/agrobiology.2022.5.933rus"
}
Radenovic, C., Maksimov, G., Kuramshina, G., Shutova, V., Hao, J., Delić, N., Sečanski, M., Popović, A., Bajuk-Bogdanovic, D., Radosavljević, M.,& Pavlov, J.. (2022). ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.). in Сельскохозяйственная биология [Agricultural Biology]
Moskva : Сельскохозяйственная биология., 57(5), 933-944.
https://doi.org/10.15389/agrobiology.2022.5.933rus
Radenovic C, Maksimov G, Kuramshina G, Shutova V, Hao J, Delić N, Sečanski M, Popović A, Bajuk-Bogdanovic D, Radosavljević M, Pavlov J. ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.). in Сельскохозяйственная биология [Agricultural Biology]. 2022;57(5):933-944.
doi:10.15389/agrobiology.2022.5.933rus .
Radenovic, Cedomir, Maksimov, Georgy, Kuramshina, Gulnara, Shutova, Vitalina, Hao, Jingrao, Delić, Nenad, Sečanski, Mile, Popović, Aleksandar, Bajuk-Bogdanovic, Danica, Radosavljević, Milica, Pavlov, Jovan, "ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.)" in Сельскохозяйственная биология [Agricultural Biology], 57, no. 5 (2022):933-944,
https://doi.org/10.15389/agrobiology.2022.5.933rus . .

Grain Yield Predictions of ZP Maize Hybrids

Čamdžija, Zoran; Filipović, Milomir; Delić, Nenad; Pavlov, Jovan; Grčić, Nikola; Ristić, Danijela; Kostadinović, Marija

(2022)

TY  - CONF
AU  - Čamdžija, Zoran
AU  - Filipović, Milomir
AU  - Delić, Nenad
AU  - Pavlov, Jovan
AU  - Grčić, Nikola
AU  - Ristić, Danijela
AU  - Kostadinović, Marija
PY  - 2022
UR  - https://www.intpbc2022.org/CONGRESS-BOOK.pdf
UR  - http://rik.mrizp.rs/handle/123456789/1353
AB  - Maize hybrids are created by crossing inbreds of
opposite heterotic groups, where the discovery of
genetic distances (GD) is of great significance. With
the presence of modern breeding tools for discovering
heterotic pairs and GDs in used material an efficiency in
breeding can be increased for more precisely planned
field work.
For this study 11 crosses were made in order to
compare the prediction of data obtained by GDs
between crossed inbreds with the results of same
hybrids from field testing. Crosses were done in 2020
according to the GD suggestions which ranged from
0.386 to 0.465 to check out is it possible to predict
specific superior hybrid performance before the field
results itself. 25k SNP Ilumina Infinium Array for maize
was used to determine GD values.
As a check ZP 457-market accepted hybrid (registered
in 2018 in Republic of Serbia) was used together with
10 other hybrids. All 11 hybrids shared the same female
component (Lancaster Sure Crop heterotic group),
while male components from opposite heterotic groups
(BSSS and Iowa dent) were used. GD values of used
males ranged from 0.190 to 0.412 clearly stating one
broad heterotic group. The most famous heterotic pair
B-73 x Mo-17 as well as two Institute’s top selling
hybrids (ZP 434 and ZP 606) were also included for
GD comparison with test hybrids with the GD values of
0.488, 0.466 and 0.462 respectively.
Review of B-73 x Mo-17 through ZP 434 and ZP 606
to the newly created hybrids suggests GD values are
decreasing with every new cycle of selection between
crossed material. Results from field testing compared
to GD values show somewhat positive correlation as
for ZP 3 exp which ranked 2nd highest GD value and
3rd rank in grain yield outyielding ZP 457 significantly,
but ZP 457 GD value of 0.458 (ranking third in GD)
suggests higher yielder should be though to find. Grain
yield results proved same hybrid ZP 457 to be one
of the lowest yielders being superior only to ZP 4123
despite high GD value. In top three yielders GD values
were in great correspondence for only one mentioned
combination ZP 3 exp, but for the ZP 4 exp and ZP
457 taking first and third position according to the GD
rank proved to be good only for 5th and 10th rank
within field results, while the lowest yielder ZP 4123
took 4th position according to GD values, having very
high value of 0.457.
This result proves SNP to be powerful tool in predicting
herotic pairs in maize and must be considered for
more precise planned field work. Even though GD
values being so informative on one side they do not
specifically imply to the solution for detecting the most
superior hybrid combination therefore leaving field
observations to confirm start assumptions.
C3  - 4. international plant breeding congress; Plant breeding for the future: from local to global, Antalya Türkiye, 21-25.11.2022. - Book of abstracts
T1  - Grain Yield Predictions of ZP Maize Hybrids
SP  - 111
EP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_rik_1353
ER  - 
@conference{
author = "Čamdžija, Zoran and Filipović, Milomir and Delić, Nenad and Pavlov, Jovan and Grčić, Nikola and Ristić, Danijela and Kostadinović, Marija",
year = "2022",
abstract = "Maize hybrids are created by crossing inbreds of
opposite heterotic groups, where the discovery of
genetic distances (GD) is of great significance. With
the presence of modern breeding tools for discovering
heterotic pairs and GDs in used material an efficiency in
breeding can be increased for more precisely planned
field work.
For this study 11 crosses were made in order to
compare the prediction of data obtained by GDs
between crossed inbreds with the results of same
hybrids from field testing. Crosses were done in 2020
according to the GD suggestions which ranged from
0.386 to 0.465 to check out is it possible to predict
specific superior hybrid performance before the field
results itself. 25k SNP Ilumina Infinium Array for maize
was used to determine GD values.
As a check ZP 457-market accepted hybrid (registered
in 2018 in Republic of Serbia) was used together with
10 other hybrids. All 11 hybrids shared the same female
component (Lancaster Sure Crop heterotic group),
while male components from opposite heterotic groups
(BSSS and Iowa dent) were used. GD values of used
males ranged from 0.190 to 0.412 clearly stating one
broad heterotic group. The most famous heterotic pair
B-73 x Mo-17 as well as two Institute’s top selling
hybrids (ZP 434 and ZP 606) were also included for
GD comparison with test hybrids with the GD values of
0.488, 0.466 and 0.462 respectively.
Review of B-73 x Mo-17 through ZP 434 and ZP 606
to the newly created hybrids suggests GD values are
decreasing with every new cycle of selection between
crossed material. Results from field testing compared
to GD values show somewhat positive correlation as
for ZP 3 exp which ranked 2nd highest GD value and
3rd rank in grain yield outyielding ZP 457 significantly,
but ZP 457 GD value of 0.458 (ranking third in GD)
suggests higher yielder should be though to find. Grain
yield results proved same hybrid ZP 457 to be one
of the lowest yielders being superior only to ZP 4123
despite high GD value. In top three yielders GD values
were in great correspondence for only one mentioned
combination ZP 3 exp, but for the ZP 4 exp and ZP
457 taking first and third position according to the GD
rank proved to be good only for 5th and 10th rank
within field results, while the lowest yielder ZP 4123
took 4th position according to GD values, having very
high value of 0.457.
This result proves SNP to be powerful tool in predicting
herotic pairs in maize and must be considered for
more precise planned field work. Even though GD
values being so informative on one side they do not
specifically imply to the solution for detecting the most
superior hybrid combination therefore leaving field
observations to confirm start assumptions.",
journal = "4. international plant breeding congress; Plant breeding for the future: from local to global, Antalya Türkiye, 21-25.11.2022. - Book of abstracts",
title = "Grain Yield Predictions of ZP Maize Hybrids",
pages = "111-111",
url = "https://hdl.handle.net/21.15107/rcub_rik_1353"
}
Čamdžija, Z., Filipović, M., Delić, N., Pavlov, J., Grčić, N., Ristić, D.,& Kostadinović, M.. (2022). Grain Yield Predictions of ZP Maize Hybrids. in 4. international plant breeding congress; Plant breeding for the future: from local to global, Antalya Türkiye, 21-25.11.2022. - Book of abstracts, 111-111.
https://hdl.handle.net/21.15107/rcub_rik_1353
Čamdžija Z, Filipović M, Delić N, Pavlov J, Grčić N, Ristić D, Kostadinović M. Grain Yield Predictions of ZP Maize Hybrids. in 4. international plant breeding congress; Plant breeding for the future: from local to global, Antalya Türkiye, 21-25.11.2022. - Book of abstracts. 2022;:111-111.
https://hdl.handle.net/21.15107/rcub_rik_1353 .
Čamdžija, Zoran, Filipović, Milomir, Delić, Nenad, Pavlov, Jovan, Grčić, Nikola, Ristić, Danijela, Kostadinović, Marija, "Grain Yield Predictions of ZP Maize Hybrids" in 4. international plant breeding congress; Plant breeding for the future: from local to global, Antalya Türkiye, 21-25.11.2022. - Book of abstracts (2022):111-111,
https://hdl.handle.net/21.15107/rcub_rik_1353 .

Population structure and diversity in former ZP maize breeding program assessed with SNP markers

Grčić, Nikola; Nikolić, Ana; Delić, Nenad; Mladenović, Marko; Čamdžija, Zoran; Božinović, Sofija; Mladenović Drinić, Snežana

(Belgrade : Maize Research Institute Zemun Polje, 2022)

TY  - CONF
AU  - Grčić, Nikola
AU  - Nikolić, Ana
AU  - Delić, Nenad
AU  - Mladenović, Marko
AU  - Čamdžija, Zoran
AU  - Božinović, Sofija
AU  - Mladenović Drinić, Snežana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1287
AB  - Maize breeding in Maize research institute “Zemun Polje” was conducted for more than 75 years resulting in the creations of numerous maize hybrids with superior traits and performance. The source breeding material used in the breeding programs was very diverse and varied from the domestic landraces to the introduced inbred lines. This resulted in a very diverse and complex structure of the breeding pools used in commercial maize breeding in “Zemun Polje” Institute. As genetic material in this study, 24 maize inbred lines of different origin, heterotic and maturity goups were selected were selected from the 3rd and 4th cycle of breeding in Maize research institute “Zemun Polje”. These are inbreds that were commonly used in breeding programs during the 1980s and were parental components of most important commercial maize hybrids. The genotyping was performed using a Maize 25k Infinium array. The neighbor-joining cladogram, PcoA and Structure were used to analyze the genetic structure and diversity of the panel. Population structure analysis of the panel initially showed a clear divide between Lancaster and Non-Lancaster inbred lines, revealing the dominant heterotic pattern in the breeding program. Further analysis with an increased number of population (k) showed a more complex structure of the non-Lancaster group of inbred lines with best number of ancestral population being k=5. The average genetic distance in the panel was 0,385, ranging from 0,039 to 0,544, with paternal inbreds in most of commercial hybrids being the most distant ones. The genotyping results of the Zemun Polje maize panel were further merged with DROPS European panel of 247 dent maize inbred lines in order to view the results of the diversity analysis of the Zemun Polje panel from a broader perspective.
PB  - Belgrade : Maize Research Institute Zemun Polje
C3  - XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts
T1  - Population structure and diversity in former ZP maize breeding program assessed with SNP markers
SP  - 32
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_rik_1287
ER  - 
@conference{
author = "Grčić, Nikola and Nikolić, Ana and Delić, Nenad and Mladenović, Marko and Čamdžija, Zoran and Božinović, Sofija and Mladenović Drinić, Snežana",
year = "2022",
abstract = "Maize breeding in Maize research institute “Zemun Polje” was conducted for more than 75 years resulting in the creations of numerous maize hybrids with superior traits and performance. The source breeding material used in the breeding programs was very diverse and varied from the domestic landraces to the introduced inbred lines. This resulted in a very diverse and complex structure of the breeding pools used in commercial maize breeding in “Zemun Polje” Institute. As genetic material in this study, 24 maize inbred lines of different origin, heterotic and maturity goups were selected were selected from the 3rd and 4th cycle of breeding in Maize research institute “Zemun Polje”. These are inbreds that were commonly used in breeding programs during the 1980s and were parental components of most important commercial maize hybrids. The genotyping was performed using a Maize 25k Infinium array. The neighbor-joining cladogram, PcoA and Structure were used to analyze the genetic structure and diversity of the panel. Population structure analysis of the panel initially showed a clear divide between Lancaster and Non-Lancaster inbred lines, revealing the dominant heterotic pattern in the breeding program. Further analysis with an increased number of population (k) showed a more complex structure of the non-Lancaster group of inbred lines with best number of ancestral population being k=5. The average genetic distance in the panel was 0,385, ranging from 0,039 to 0,544, with paternal inbreds in most of commercial hybrids being the most distant ones. The genotyping results of the Zemun Polje maize panel were further merged with DROPS European panel of 247 dent maize inbred lines in order to view the results of the diversity analysis of the Zemun Polje panel from a broader perspective.",
publisher = "Belgrade : Maize Research Institute Zemun Polje",
journal = "XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts",
title = "Population structure and diversity in former ZP maize breeding program assessed with SNP markers",
pages = "32-32",
url = "https://hdl.handle.net/21.15107/rcub_rik_1287"
}
Grčić, N., Nikolić, A., Delić, N., Mladenović, M., Čamdžija, Z., Božinović, S.,& Mladenović Drinić, S.. (2022). Population structure and diversity in former ZP maize breeding program assessed with SNP markers. in XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts
Belgrade : Maize Research Institute Zemun Polje., 32-32.
https://hdl.handle.net/21.15107/rcub_rik_1287
Grčić N, Nikolić A, Delić N, Mladenović M, Čamdžija Z, Božinović S, Mladenović Drinić S. Population structure and diversity in former ZP maize breeding program assessed with SNP markers. in XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts. 2022;:32-32.
https://hdl.handle.net/21.15107/rcub_rik_1287 .
Grčić, Nikola, Nikolić, Ana, Delić, Nenad, Mladenović, Marko, Čamdžija, Zoran, Božinović, Sofija, Mladenović Drinić, Snežana, "Population structure and diversity in former ZP maize breeding program assessed with SNP markers" in XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts (2022):32-32,
https://hdl.handle.net/21.15107/rcub_rik_1287 .

Suitability of maize hybrids for the enrichment of marinated baby corn with anthocyanins extracted from black soybean

Nikolić, Valentina; Simić, Marijana; Žilić, Slađana; Perić, Vesna; Srdić, Jelena; Pavlov, Jovan; Delić, Nenad

(Belgrade : Maize Research Institute "Zemun Polje", 2022)

TY  - CONF
AU  - Nikolić, Valentina
AU  - Simić, Marijana
AU  - Žilić, Slađana
AU  - Perić, Vesna
AU  - Srdić, Jelena
AU  - Pavlov, Jovan
AU  - Delić, Nenad
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1186
AB  - Maize (Zea mays L.) is the third most grown cereal crop in the world, after wheat and rice. Immature dehusked maize ears harvested before fertilization, within 2-4 days after white silk emergence are classified as baby corn. The objective of this study was to investigate the suitability of baby corn cobs obtained from different maize hybrids for their enrichment with anthocyanins extracted from the black soybean seed coats. Three different maize hybrids, namely, a popcorn hybrid ZP 6119k, a sweet hybrid ZP 553su, a semi-flint with a short vegetation period (105 days) ZP 161, as well as black soybean genotype “Black Tokyo”, were used in this study. Plant material was grown in the experimental field of the Maize Research Institute at the location of Zemun Polje, Serbia in 2021. Maize was harvested after the emergence of silk from the ears (silking stage), while soybean plants were harvested in the stage of full maturity (R8). The baby maize cobs differed in length (9.5-20.0 cm) and girth (12-21 mm), the number of kernel rows (12-20), color, and length of the silk outside the husk (3-7 cm). Soybean seeds were manually dehulled and seed coat was ground in a laboratory mill prior to the extraction of anthocyanins. After dehusking and measuring, the baby corn cobs were washed and blanched for two minutes in boiling water, and then cooled. Subsequently, the baby corn cobs were immersed in a marinating brine enriched with anthocyanins extracted from the seed coat of black soybean, pasteurized, and kept in closed glass jars in a cool dry place for 30 days. After chemical and sensory analyzes, the product obtained from popcorn hybrid ZP 6119k was chosen as the most suitable for the preparation of the marinated baby corn product enriched with anthocyanins from black soybean
PB  - Belgrade : Maize Research Institute "Zemun Polje"
C3  - 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts
T1  - Suitability of maize hybrids for the enrichment of marinated baby corn with anthocyanins extracted from black soybean
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_rik_1186
ER  - 
@conference{
author = "Nikolić, Valentina and Simić, Marijana and Žilić, Slađana and Perić, Vesna and Srdić, Jelena and Pavlov, Jovan and Delić, Nenad",
year = "2022",
abstract = "Maize (Zea mays L.) is the third most grown cereal crop in the world, after wheat and rice. Immature dehusked maize ears harvested before fertilization, within 2-4 days after white silk emergence are classified as baby corn. The objective of this study was to investigate the suitability of baby corn cobs obtained from different maize hybrids for their enrichment with anthocyanins extracted from the black soybean seed coats. Three different maize hybrids, namely, a popcorn hybrid ZP 6119k, a sweet hybrid ZP 553su, a semi-flint with a short vegetation period (105 days) ZP 161, as well as black soybean genotype “Black Tokyo”, were used in this study. Plant material was grown in the experimental field of the Maize Research Institute at the location of Zemun Polje, Serbia in 2021. Maize was harvested after the emergence of silk from the ears (silking stage), while soybean plants were harvested in the stage of full maturity (R8). The baby maize cobs differed in length (9.5-20.0 cm) and girth (12-21 mm), the number of kernel rows (12-20), color, and length of the silk outside the husk (3-7 cm). Soybean seeds were manually dehulled and seed coat was ground in a laboratory mill prior to the extraction of anthocyanins. After dehusking and measuring, the baby corn cobs were washed and blanched for two minutes in boiling water, and then cooled. Subsequently, the baby corn cobs were immersed in a marinating brine enriched with anthocyanins extracted from the seed coat of black soybean, pasteurized, and kept in closed glass jars in a cool dry place for 30 days. After chemical and sensory analyzes, the product obtained from popcorn hybrid ZP 6119k was chosen as the most suitable for the preparation of the marinated baby corn product enriched with anthocyanins from black soybean",
publisher = "Belgrade : Maize Research Institute "Zemun Polje"",
journal = "25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts",
title = "Suitability of maize hybrids for the enrichment of marinated baby corn with anthocyanins extracted from black soybean",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_rik_1186"
}
Nikolić, V., Simić, M., Žilić, S., Perić, V., Srdić, J., Pavlov, J.,& Delić, N.. (2022). Suitability of maize hybrids for the enrichment of marinated baby corn with anthocyanins extracted from black soybean. in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts
Belgrade : Maize Research Institute "Zemun Polje"., 36.
https://hdl.handle.net/21.15107/rcub_rik_1186
Nikolić V, Simić M, Žilić S, Perić V, Srdić J, Pavlov J, Delić N. Suitability of maize hybrids for the enrichment of marinated baby corn with anthocyanins extracted from black soybean. in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts. 2022;:36.
https://hdl.handle.net/21.15107/rcub_rik_1186 .
Nikolić, Valentina, Simić, Marijana, Žilić, Slađana, Perić, Vesna, Srdić, Jelena, Pavlov, Jovan, Delić, Nenad, "Suitability of maize hybrids for the enrichment of marinated baby corn with anthocyanins extracted from black soybean" in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts (2022):36,
https://hdl.handle.net/21.15107/rcub_rik_1186 .

Chilling induced stress sRNAs in maize plants in early stages of development.

Božić, Manja; Ignjatović-Micić, Dragana; Vančetović, Jelena; Delić, Nenad; Nikolić, Ana

(Belgrade : Serbian Plant Physiology Society, 2022)

TY  - CONF
AU  - Božić, Manja
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1152
AB  - Small non-coding RNAs (sRNAs) have an important role in regulating plant gene expression,
both in developmental processes and stress responses, through RNA interference or silencing.
sRNAs respond to environmental stressors in manners dependant on the type of stress, tissue
and genetic background. Herein, their role in chilling stress response is examined in young maize
seedlings (Zea mays L.) of two inbred lines of contrasting chilling tolerance, using high-throughput
sequencing methods. Understanding this response is critical since one of the most promising
strategies of avoiding maize yield loss due to climate change is earlier sowing, during periods
of suboptimal temperatures. Sterilized seeds of the two lines, marked as LT (tolerant), and LS
(sensitive), were germinated in the dark for five days (optimal conditions), after which the 5-d
old seedlings were exposed to chilling conditions for 24h (temperature: 10/8 °C; photoperiod:
12/12h). Samples for RNA isolation were taken after 6h and 24h of treatment. After the cDNA
library preparations were finished, single-end 50 bp sequencing was performed (Illumina® Novaseq 6000). sRNAseq analysis was performed using a custom pipeline that included quality control, mapping, known miRNA alignment and novel miRNA prediction and quantification. This is an ongoing research and a selection of results will be presented. On average 81% of sRNA reads were succesfully mapped and belong to the class of microRNAs (miRNAs). Some of the sRNAs appear to be differentialy regulated depending on the genotype and the chilling conditions, but more research is needed to verify their potential role in this process.
PB  - Belgrade : Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - Chilling induced stress sRNAs in maize plants in early stages of development.
UR  - https://hdl.handle.net/21.15107/rcub_rik_1152
ER  - 
@conference{
author = "Božić, Manja and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2022",
abstract = "Small non-coding RNAs (sRNAs) have an important role in regulating plant gene expression,
both in developmental processes and stress responses, through RNA interference or silencing.
sRNAs respond to environmental stressors in manners dependant on the type of stress, tissue
and genetic background. Herein, their role in chilling stress response is examined in young maize
seedlings (Zea mays L.) of two inbred lines of contrasting chilling tolerance, using high-throughput
sequencing methods. Understanding this response is critical since one of the most promising
strategies of avoiding maize yield loss due to climate change is earlier sowing, during periods
of suboptimal temperatures. Sterilized seeds of the two lines, marked as LT (tolerant), and LS
(sensitive), were germinated in the dark for five days (optimal conditions), after which the 5-d
old seedlings were exposed to chilling conditions for 24h (temperature: 10/8 °C; photoperiod:
12/12h). Samples for RNA isolation were taken after 6h and 24h of treatment. After the cDNA
library preparations were finished, single-end 50 bp sequencing was performed (Illumina® Novaseq 6000). sRNAseq analysis was performed using a custom pipeline that included quality control, mapping, known miRNA alignment and novel miRNA prediction and quantification. This is an ongoing research and a selection of results will be presented. On average 81% of sRNA reads were succesfully mapped and belong to the class of microRNAs (miRNAs). Some of the sRNAs appear to be differentialy regulated depending on the genotype and the chilling conditions, but more research is needed to verify their potential role in this process.",
publisher = "Belgrade : Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "Chilling induced stress sRNAs in maize plants in early stages of development.",
url = "https://hdl.handle.net/21.15107/rcub_rik_1152"
}
Božić, M., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Nikolić, A.. (2022). Chilling induced stress sRNAs in maize plants in early stages of development.. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Belgrade : Serbian Plant Physiology Society..
https://hdl.handle.net/21.15107/rcub_rik_1152
Božić M, Ignjatović-Micić D, Vančetović J, Delić N, Nikolić A. Chilling induced stress sRNAs in maize plants in early stages of development.. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1152 .
Božić, Manja, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "Chilling induced stress sRNAs in maize plants in early stages of development." in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022),
https://hdl.handle.net/21.15107/rcub_rik_1152 .

circRNAs involved in chilling stress response during early developmental stages in maize

Božić, Manja; Ignjatović-Micić, Dragana; Vančetović, Jelena; Delić, Nenad; Nikolić, Ana

(2022)

TY  - GEN
AU  - Božić, Manja
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1139
AB  - Circular RNAs (circRNAs) are endogenous, single-stranded, covalently closed RNA molecules, generated by back-splicing events. Their role as regulators of different processes in animal cells has been widely studied and proven, however functional studies on circRNAs in plants are still few and far between. Here we attempt to bring light to their role in abiotic stress response – particularly chilling stress, in young maize seedlings (Zea mays L.) using high-throughput sequencing methods. One of the strategies of avoiding yield loss is earlier maize sowing as to escape exposure to extremely high temperatures and drought in the summer. This means submitting maize plants to suboptimal temperatures during earlier developmental stages.
Two maize inbred lines of contrasting tolerance to chilling were selected and marked as LT (tolerant), and LS (sensitive). Sterilized seeds of the two lines were germinated for five days, in the dark, in optimal conditions and after the germination period was finished, the 5-d old seedlings were exposed to chilling conditions for 24h (temperature: 10/8° C; photoperiod: 12/12h). Sampling was done after 6h and 24h of treatment. After total RNA isolation and purification, quality check, and cDNA library preparations were finished, paired-end 150 sequencing was performed (Illumina® Novaseq 6000). Custom pipeline was used for circRNAseq data analysis and included their identification and quantification (CIRCexplorer2, v2.0.5), differential expression analysis (edgeR Bioconductor v4.1) and functional enrichment (GO, KEGG, psRobot). 
Processing of this data is still underway, and by now several thousand circRNA molecules and over a thousand 5' and 3' alternative back-splicing sites have been identified. Significant number of those circRNA have shown expression dependent on chilling, as well as genotype, but further studies are necessary to confirm their role in this process, find their target molecules and unravel regulatory networks they create with other types of RNAs.
T2  - 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts
T1  - circRNAs involved in chilling stress response during early developmental stages in maize
UR  - https://hdl.handle.net/21.15107/rcub_rik_1139
ER  - 
@misc{
author = "Božić, Manja and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2022",
abstract = "Circular RNAs (circRNAs) are endogenous, single-stranded, covalently closed RNA molecules, generated by back-splicing events. Their role as regulators of different processes in animal cells has been widely studied and proven, however functional studies on circRNAs in plants are still few and far between. Here we attempt to bring light to their role in abiotic stress response – particularly chilling stress, in young maize seedlings (Zea mays L.) using high-throughput sequencing methods. One of the strategies of avoiding yield loss is earlier maize sowing as to escape exposure to extremely high temperatures and drought in the summer. This means submitting maize plants to suboptimal temperatures during earlier developmental stages.
Two maize inbred lines of contrasting tolerance to chilling were selected and marked as LT (tolerant), and LS (sensitive). Sterilized seeds of the two lines were germinated for five days, in the dark, in optimal conditions and after the germination period was finished, the 5-d old seedlings were exposed to chilling conditions for 24h (temperature: 10/8° C; photoperiod: 12/12h). Sampling was done after 6h and 24h of treatment. After total RNA isolation and purification, quality check, and cDNA library preparations were finished, paired-end 150 sequencing was performed (Illumina® Novaseq 6000). Custom pipeline was used for circRNAseq data analysis and included their identification and quantification (CIRCexplorer2, v2.0.5), differential expression analysis (edgeR Bioconductor v4.1) and functional enrichment (GO, KEGG, psRobot). 
Processing of this data is still underway, and by now several thousand circRNA molecules and over a thousand 5' and 3' alternative back-splicing sites have been identified. Significant number of those circRNA have shown expression dependent on chilling, as well as genotype, but further studies are necessary to confirm their role in this process, find their target molecules and unravel regulatory networks they create with other types of RNAs.",
journal = "25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts",
title = "circRNAs involved in chilling stress response during early developmental stages in maize",
url = "https://hdl.handle.net/21.15107/rcub_rik_1139"
}
Božić, M., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Nikolić, A.. (2022). circRNAs involved in chilling stress response during early developmental stages in maize. in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts.
https://hdl.handle.net/21.15107/rcub_rik_1139
Božić M, Ignjatović-Micić D, Vančetović J, Delić N, Nikolić A. circRNAs involved in chilling stress response during early developmental stages in maize. in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1139 .
Božić, Manja, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "circRNAs involved in chilling stress response during early developmental stages in maize" in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_rik_1139 .

ZP5797

Delić, Nenad; Pavlov, Jovan

(2022)

TY  - PAT
AU  - Delić, Nenad
AU  - Pavlov, Jovan
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1106
T2  - Rešenje:320-44-02147/2/2020-11
T1  - ZP5797
UR  - https://hdl.handle.net/21.15107/rcub_rik_1106
ER  - 
@misc{
author = "Delić, Nenad and Pavlov, Jovan",
year = "2022",
journal = "Rešenje:320-44-02147/2/2020-11",
title = "ZP5797",
url = "https://hdl.handle.net/21.15107/rcub_rik_1106"
}
Delić, N.,& Pavlov, J.. (2022). ZP5797. in Rešenje:320-44-02147/2/2020-11.
https://hdl.handle.net/21.15107/rcub_rik_1106
Delić N, Pavlov J. ZP5797. in Rešenje:320-44-02147/2/2020-11. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1106 .
Delić, Nenad, Pavlov, Jovan, "ZP5797" in Rešenje:320-44-02147/2/2020-11 (2022),
https://hdl.handle.net/21.15107/rcub_rik_1106 .

ZP5588

Delić, Nenad; Pavlov, Jovan

(2022)

TY  - PAT
AU  - Delić, Nenad
AU  - Pavlov, Jovan
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1105
T2  - Rešenje:320-44-02149/2/2020-11
T1  - ZP5588
UR  - https://hdl.handle.net/21.15107/rcub_rik_1105
ER  - 
@misc{
author = "Delić, Nenad and Pavlov, Jovan",
year = "2022",
journal = "Rešenje:320-44-02149/2/2020-11",
title = "ZP5588",
url = "https://hdl.handle.net/21.15107/rcub_rik_1105"
}
Delić, N.,& Pavlov, J.. (2022). ZP5588. in Rešenje:320-44-02149/2/2020-11.
https://hdl.handle.net/21.15107/rcub_rik_1105
Delić N, Pavlov J. ZP5588. in Rešenje:320-44-02149/2/2020-11. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1105 .
Delić, Nenad, Pavlov, Jovan, "ZP5588" in Rešenje:320-44-02149/2/2020-11 (2022),
https://hdl.handle.net/21.15107/rcub_rik_1105 .

ZP5252

Stevanović, Milan; Delić, Nenad; Filipović, Milomir; Stanković, Goran

(2022)

TY  - PAT
AU  - Stevanović, Milan
AU  - Delić, Nenad
AU  - Filipović, Milomir
AU  - Stanković, Goran
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1104
T2  - Rešenje:320-44-02165/2/2020-11
T1  - ZP5252
UR  - https://hdl.handle.net/21.15107/rcub_rik_1104
ER  - 
@misc{
author = "Stevanović, Milan and Delić, Nenad and Filipović, Milomir and Stanković, Goran",
year = "2022",
journal = "Rešenje:320-44-02165/2/2020-11",
title = "ZP5252",
url = "https://hdl.handle.net/21.15107/rcub_rik_1104"
}
Stevanović, M., Delić, N., Filipović, M.,& Stanković, G.. (2022). ZP5252. in Rešenje:320-44-02165/2/2020-11.
https://hdl.handle.net/21.15107/rcub_rik_1104
Stevanović M, Delić N, Filipović M, Stanković G. ZP5252. in Rešenje:320-44-02165/2/2020-11. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1104 .
Stevanović, Milan, Delić, Nenad, Filipović, Milomir, Stanković, Goran, "ZP5252" in Rešenje:320-44-02165/2/2020-11 (2022),
https://hdl.handle.net/21.15107/rcub_rik_1104 .

ZP5191

Grčić, Nikola; Todorović, Goran; Delić, Nenad; Mladenović, Marko

(2022)

TY  - PAT
AU  - Grčić, Nikola
AU  - Todorović, Goran
AU  - Delić, Nenad
AU  - Mladenović, Marko
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1103
T2  - Rešenje:320-44-02145/2/2020-11
T1  - ZP5191
UR  - https://hdl.handle.net/21.15107/rcub_rik_1103
ER  - 
@misc{
author = "Grčić, Nikola and Todorović, Goran and Delić, Nenad and Mladenović, Marko",
year = "2022",
journal = "Rešenje:320-44-02145/2/2020-11",
title = "ZP5191",
url = "https://hdl.handle.net/21.15107/rcub_rik_1103"
}
Grčić, N., Todorović, G., Delić, N.,& Mladenović, M.. (2022). ZP5191. in Rešenje:320-44-02145/2/2020-11.
https://hdl.handle.net/21.15107/rcub_rik_1103
Grčić N, Todorović G, Delić N, Mladenović M. ZP5191. in Rešenje:320-44-02145/2/2020-11. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1103 .
Grčić, Nikola, Todorović, Goran, Delić, Nenad, Mladenović, Marko, "ZP5191" in Rešenje:320-44-02145/2/2020-11 (2022),
https://hdl.handle.net/21.15107/rcub_rik_1103 .

Nutritional and cost effects of adapted quality protein maize on broiler feeding

Kostadinović, Marija; Ristić, Danijela; Vančetović, Jelena; Delić, Nenad; Ignjatović-Micić, Dragana

(Belgrade : Maize Research Institute, Zemun Polje, 2022)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Ignjatović-Micić, Dragana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/967
AB  - ZPQPM13 is a maize hybrid with improved protein quality, adapted to temperate climate growth conditions, developed by crossing a QPM version of ZPL5 commercial line (developed by marker assisted selection) and GS-6 (a QPM line with 50% tropical and 50% temperate germplasm). This hybrid has grain yield as commercial hybrids, over 90% of hard endosperm, contents of tryptophan and protein over 0.075% and 10%, respectively, and stability of tryptophan content over different locations and years. ZPQPM13 was tested for use in broiler feeds, with the aim to reduce utilization of costly synthetic lysine or soybean components. The feeding experiment on Ross 308 broilers was divided into two groups – control (fed with standard maize) and treatment (fed with QPM). In treatment group diets, ZPQPM13 was increased and soybean component decreased for 3%. Chemical analyses revealed higher contents of limiting essential amino acids in mercantile ZPQPM13 compared to standard maize – 0.44 vs. 0.25 % lysine, 0.077 vs. 0.070 % tryptophan and 0.39 vs. 0.27 % threonine. Duration of each feeding trial was 42 days, comprising three phases - starter (1-14 days), grower (15-35 days) and finisher (36-42 days). Feed intake (FI), body mass gain (BMG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. In treatment group, FI was reduced, while BMG and ADG were higher in starter phase but lower in grower and finisher phases. FCR was lower in all three phases in treatment group – 1.13 vs. 1.30, 1.25 vs. 1.33 and 1.28 vs. 1.45. Although a small drawback is that final weight was 3.4 % higher in control broilers, comparison of feed and meat prices show that financial benefits of using QPM are still significant compared to using standard maize in feeds, as soybean is four times more expensive than maize.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Nutritional and cost effects of adapted quality protein maize on broiler feeding
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rik_967
ER  - 
@conference{
author = "Kostadinović, Marija and Ristić, Danijela and Vančetović, Jelena and Delić, Nenad and Ignjatović-Micić, Dragana",
year = "2022",
abstract = "ZPQPM13 is a maize hybrid with improved protein quality, adapted to temperate climate growth conditions, developed by crossing a QPM version of ZPL5 commercial line (developed by marker assisted selection) and GS-6 (a QPM line with 50% tropical and 50% temperate germplasm). This hybrid has grain yield as commercial hybrids, over 90% of hard endosperm, contents of tryptophan and protein over 0.075% and 10%, respectively, and stability of tryptophan content over different locations and years. ZPQPM13 was tested for use in broiler feeds, with the aim to reduce utilization of costly synthetic lysine or soybean components. The feeding experiment on Ross 308 broilers was divided into two groups – control (fed with standard maize) and treatment (fed with QPM). In treatment group diets, ZPQPM13 was increased and soybean component decreased for 3%. Chemical analyses revealed higher contents of limiting essential amino acids in mercantile ZPQPM13 compared to standard maize – 0.44 vs. 0.25 % lysine, 0.077 vs. 0.070 % tryptophan and 0.39 vs. 0.27 % threonine. Duration of each feeding trial was 42 days, comprising three phases - starter (1-14 days), grower (15-35 days) and finisher (36-42 days). Feed intake (FI), body mass gain (BMG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. In treatment group, FI was reduced, while BMG and ADG were higher in starter phase but lower in grower and finisher phases. FCR was lower in all three phases in treatment group – 1.13 vs. 1.30, 1.25 vs. 1.33 and 1.28 vs. 1.45. Although a small drawback is that final weight was 3.4 % higher in control broilers, comparison of feed and meat prices show that financial benefits of using QPM are still significant compared to using standard maize in feeds, as soybean is four times more expensive than maize.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Nutritional and cost effects of adapted quality protein maize on broiler feeding",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_rik_967"
}
Kostadinović, M., Ristić, D., Vančetović, J., Delić, N.,& Ignjatović-Micić, D.. (2022). Nutritional and cost effects of adapted quality protein maize on broiler feeding. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 70.
https://hdl.handle.net/21.15107/rcub_rik_967
Kostadinović M, Ristić D, Vančetović J, Delić N, Ignjatović-Micić D. Nutritional and cost effects of adapted quality protein maize on broiler feeding. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;:70.
https://hdl.handle.net/21.15107/rcub_rik_967 .
Kostadinović, Marija, Ristić, Danijela, Vančetović, Jelena, Delić, Nenad, Ignjatović-Micić, Dragana, "Nutritional and cost effects of adapted quality protein maize on broiler feeding" in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022):70,
https://hdl.handle.net/21.15107/rcub_rik_967 .

ИСПОЛЬЗОВАНИЕ ИНФРАКРАСНОЙ СПЕКТРОСКОПИИ И СПЕКТРОСКОПИИ КОМБИНАЦИОННОГО РАССЕЯНИЯ ДЛЯ АНАЛИЗА СОСТОЯНИЯ БИОМОЛЕКУЛ У ЛИНИЙ КУКУРУЗЫ Zea mays L.

Radenovic, Cedomir; Maksimov, Georgij; Shutova, Vitalina; Hao, Jingrao; Delić, Nenad; Sečanski, Mile; Popović, Aleksandar; Sečanski, Mile

(Moscow : Agricultural Biology Editorial Office, NPO, 2021)

TY  - JOUR
AU  - Radenovic, Cedomir
AU  - Maksimov, Georgij
AU  - Shutova, Vitalina
AU  - Hao, Jingrao
AU  - Delić, Nenad
AU  - Sečanski, Mile
AU  - Popović, Aleksandar
AU  - Sečanski, Mile
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1357
AB  - В настоящее время мало неинвазивных методов, которые позволяют контролировать со-держание и конформацию молекул биоактивных органических соединений в клетках и тканях рас-тений не только в лабораторных, но и в полевых условиях. Инфракрасная спектроскопия и спек-троскопия комбинационного рассеяния (ИК- и КР-спектроскопия) активно используется для ана-лиза роли молекул некоторых веществ в селекции сельскохозяйственных культур. С помощью ме-тодов вибронной спектроскопии мы исследовали изменения содержания и конформации молекул хлоропластов у различных линий кукурузы. Методом ИК-спектроскопии (область 3500-3000 см−1) установлено, что в воде, углеводах и белках хлоропластов доля колебаний ОН-групп и внутри- и межмолекулярных Н-связей максимальна у линии кукурузы ZPPL 186, а колебаний NH-групп ами-дов (белки) минимальна у линии ZPPL 225. Доказано, что для линии ZPPL 186 характерна максимальная доля колебаний от молекул алканов, карбоновых кислот (область 2920-2860 см−1) и деформационных колебаний ароматических структур (полоса 1000 см−1), находящихся в хлоропла-стах, тогда как для линии M1-3-3-sdms — доля валентных колебания O=C=O связей (полоса 2300 см−1). С использованием КР-спектроскопии (области 1250-500 см−1 и 1535-1400 см−1) обна-ружено, что различия в спектрах между линиями кукурузы связаны с выявленными изменениями конформации молекул каротиноидов в хлоропластах, но не молекул целлюлозы. Установлено, что у всех образцов (кроме ZPPL 225) молекулы каротиноидов находятся в 15-trans форме с различной конформацией полиеновой цепи. Отметим, что у линии ZPPL 186 конформация каротиноидов, находящихся в хлоропластах, характеризуется минимальной величиной поворота вне плоскости полиеновой цепи и более выраженными колебаниями боковой СН3-группы. Высказывает пред-положение, что у изученных линий кукурузы каротиноиды хлоропластов листа не взаимодействуют с ароматическими аминокислотами белков. По мнению авторов, сочетание методов ИК- и КР-спектроскопии хлоропластов листа можно рекомендовать для контроля за содержанием и конфор-мациоными изменениями биомолекул при селекции кукурузы.
AB  - Currently, there are few non-invasive methods that allow you to control the content and conformation of molecules in plant cells and tissues, not only in the laboratory, but also in the field. Infrared microscopy and Raman spectroscopy (IR and Raman spectroscopy) are actively used to ana-lyze the role of molecules of certain substances in crop breeding. Using methods of vibronic spectroscopy, we investigated changes in the content and conformation of chloroplast molecules of various maize lines. Using infrared (IR) spectroscopy (3500-3000 cm−1) it was found that in the chloroplasts, the proportion of vibrations of OH-groups and intramolecular and intermolecular H bonds is maximum for the maize line ZPPL 186, and vibrations of NH-groups of amides (proteins) is minimal for ZPPL 225. It has been proven that ZPPL 186 chloroplasts are characterized by the maximum proportion of stretching vibrations from alkane molecules, carboxylic acids (region 2920-2860 cm−1) and deformation vibrations of aromatic structures (band at 1000 cm−1), and for the line M1-3-3-sdms, the fraction of stretching vibrations of O=C=O bonds (band at 2300 cm−1) is characteristic. Using Raman spectroscopy (range of 1250-500 cm−1 and 1535-1400 cm−1), it was found that differences in the chloroplasts of different maize lines are associated with changes in the conformation of chloroplast carotenoid molecules (rather than cellulose molecules). It was found that in two samples (except ZPPL 225), carotenoid molecules are in the 15-trans form with different conformation of the polyene chain. We note that the conformation of carotenoids of the ZPPL 186 line is characterized by a minimum amount of rotation outside the plane of the polyene chain and has more pronounced vibrations of the lateral CH3-group. It was assumed that carotenoids of leaf chloroplasts of various maize lines lack interactions with aromatic amino acids of proteins. According to the authors, the combined use of IR and Raman spectroscopy of the leaf chloroplast fraction can be recommended for monitoring the content and conformation of biomolecules in maize breeding.
PB  - Moscow : Agricultural Biology Editorial Office, NPO
T2  - Sel'skokhozyaistvennaya Biologiya [Agricultural Biology]
T1  - ИСПОЛЬЗОВАНИЕ ИНФРАКРАСНОЙ СПЕКТРОСКОПИИ И СПЕКТРОСКОПИИ КОМБИНАЦИОННОГО РАССЕЯНИЯ ДЛЯ АНАЛИЗА СОСТОЯНИЯ БИОМОЛЕКУЛ У ЛИНИЙ КУКУРУЗЫ Zea mays L.
T1  - USING INFRARED SPECTROSCOPY AND RAMAN SPECTROSCOPY TO EVALUATE THE CONFORMATION OF BIOMOLECULES IN MAIZE (Zea mays L.) LINES
VL  - 56
IS  - 5
SP  - 948
EP  - 957
DO  - 10.15389/agrobiology.2021.5.948rus
ER  - 
@article{
author = "Radenovic, Cedomir and Maksimov, Georgij and Shutova, Vitalina and Hao, Jingrao and Delić, Nenad and Sečanski, Mile and Popović, Aleksandar and Sečanski, Mile",
year = "2021",
abstract = "В настоящее время мало неинвазивных методов, которые позволяют контролировать со-держание и конформацию молекул биоактивных органических соединений в клетках и тканях рас-тений не только в лабораторных, но и в полевых условиях. Инфракрасная спектроскопия и спек-троскопия комбинационного рассеяния (ИК- и КР-спектроскопия) активно используется для ана-лиза роли молекул некоторых веществ в селекции сельскохозяйственных культур. С помощью ме-тодов вибронной спектроскопии мы исследовали изменения содержания и конформации молекул хлоропластов у различных линий кукурузы. Методом ИК-спектроскопии (область 3500-3000 см−1) установлено, что в воде, углеводах и белках хлоропластов доля колебаний ОН-групп и внутри- и межмолекулярных Н-связей максимальна у линии кукурузы ZPPL 186, а колебаний NH-групп ами-дов (белки) минимальна у линии ZPPL 225. Доказано, что для линии ZPPL 186 характерна максимальная доля колебаний от молекул алканов, карбоновых кислот (область 2920-2860 см−1) и деформационных колебаний ароматических структур (полоса 1000 см−1), находящихся в хлоропла-стах, тогда как для линии M1-3-3-sdms — доля валентных колебания O=C=O связей (полоса 2300 см−1). С использованием КР-спектроскопии (области 1250-500 см−1 и 1535-1400 см−1) обна-ружено, что различия в спектрах между линиями кукурузы связаны с выявленными изменениями конформации молекул каротиноидов в хлоропластах, но не молекул целлюлозы. Установлено, что у всех образцов (кроме ZPPL 225) молекулы каротиноидов находятся в 15-trans форме с различной конформацией полиеновой цепи. Отметим, что у линии ZPPL 186 конформация каротиноидов, находящихся в хлоропластах, характеризуется минимальной величиной поворота вне плоскости полиеновой цепи и более выраженными колебаниями боковой СН3-группы. Высказывает пред-положение, что у изученных линий кукурузы каротиноиды хлоропластов листа не взаимодействуют с ароматическими аминокислотами белков. По мнению авторов, сочетание методов ИК- и КР-спектроскопии хлоропластов листа можно рекомендовать для контроля за содержанием и конфор-мациоными изменениями биомолекул при селекции кукурузы., Currently, there are few non-invasive methods that allow you to control the content and conformation of molecules in plant cells and tissues, not only in the laboratory, but also in the field. Infrared microscopy and Raman spectroscopy (IR and Raman spectroscopy) are actively used to ana-lyze the role of molecules of certain substances in crop breeding. Using methods of vibronic spectroscopy, we investigated changes in the content and conformation of chloroplast molecules of various maize lines. Using infrared (IR) spectroscopy (3500-3000 cm−1) it was found that in the chloroplasts, the proportion of vibrations of OH-groups and intramolecular and intermolecular H bonds is maximum for the maize line ZPPL 186, and vibrations of NH-groups of amides (proteins) is minimal for ZPPL 225. It has been proven that ZPPL 186 chloroplasts are characterized by the maximum proportion of stretching vibrations from alkane molecules, carboxylic acids (region 2920-2860 cm−1) and deformation vibrations of aromatic structures (band at 1000 cm−1), and for the line M1-3-3-sdms, the fraction of stretching vibrations of O=C=O bonds (band at 2300 cm−1) is characteristic. Using Raman spectroscopy (range of 1250-500 cm−1 and 1535-1400 cm−1), it was found that differences in the chloroplasts of different maize lines are associated with changes in the conformation of chloroplast carotenoid molecules (rather than cellulose molecules). It was found that in two samples (except ZPPL 225), carotenoid molecules are in the 15-trans form with different conformation of the polyene chain. We note that the conformation of carotenoids of the ZPPL 186 line is characterized by a minimum amount of rotation outside the plane of the polyene chain and has more pronounced vibrations of the lateral CH3-group. It was assumed that carotenoids of leaf chloroplasts of various maize lines lack interactions with aromatic amino acids of proteins. According to the authors, the combined use of IR and Raman spectroscopy of the leaf chloroplast fraction can be recommended for monitoring the content and conformation of biomolecules in maize breeding.",
publisher = "Moscow : Agricultural Biology Editorial Office, NPO",
journal = "Sel'skokhozyaistvennaya Biologiya [Agricultural Biology]",
title = "ИСПОЛЬЗОВАНИЕ ИНФРАКРАСНОЙ СПЕКТРОСКОПИИ И СПЕКТРОСКОПИИ КОМБИНАЦИОННОГО РАССЕЯНИЯ ДЛЯ АНАЛИЗА СОСТОЯНИЯ БИОМОЛЕКУЛ У ЛИНИЙ КУКУРУЗЫ Zea mays L., USING INFRARED SPECTROSCOPY AND RAMAN SPECTROSCOPY TO EVALUATE THE CONFORMATION OF BIOMOLECULES IN MAIZE (Zea mays L.) LINES",
volume = "56",
number = "5",
pages = "948-957",
doi = "10.15389/agrobiology.2021.5.948rus"
}
Radenovic, C., Maksimov, G., Shutova, V., Hao, J., Delić, N., Sečanski, M., Popović, A.,& Sečanski, M.. (2021). ИСПОЛЬЗОВАНИЕ ИНФРАКРАСНОЙ СПЕКТРОСКОПИИ И СПЕКТРОСКОПИИ КОМБИНАЦИОННОГО РАССЕЯНИЯ ДЛЯ АНАЛИЗА СОСТОЯНИЯ БИОМОЛЕКУЛ У ЛИНИЙ КУКУРУЗЫ Zea mays L.. in Sel'skokhozyaistvennaya Biologiya [Agricultural Biology]
Moscow : Agricultural Biology Editorial Office, NPO., 56(5), 948-957.
https://doi.org/10.15389/agrobiology.2021.5.948rus
Radenovic C, Maksimov G, Shutova V, Hao J, Delić N, Sečanski M, Popović A, Sečanski M. ИСПОЛЬЗОВАНИЕ ИНФРАКРАСНОЙ СПЕКТРОСКОПИИ И СПЕКТРОСКОПИИ КОМБИНАЦИОННОГО РАССЕЯНИЯ ДЛЯ АНАЛИЗА СОСТОЯНИЯ БИОМОЛЕКУЛ У ЛИНИЙ КУКУРУЗЫ Zea mays L.. in Sel'skokhozyaistvennaya Biologiya [Agricultural Biology]. 2021;56(5):948-957.
doi:10.15389/agrobiology.2021.5.948rus .
Radenovic, Cedomir, Maksimov, Georgij, Shutova, Vitalina, Hao, Jingrao, Delić, Nenad, Sečanski, Mile, Popović, Aleksandar, Sečanski, Mile, "ИСПОЛЬЗОВАНИЕ ИНФРАКРАСНОЙ СПЕКТРОСКОПИИ И СПЕКТРОСКОПИИ КОМБИНАЦИОННОГО РАССЕЯНИЯ ДЛЯ АНАЛИЗА СОСТОЯНИЯ БИОМОЛЕКУЛ У ЛИНИЙ КУКУРУЗЫ Zea mays L." in Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 56, no. 5 (2021):948-957,
https://doi.org/10.15389/agrobiology.2021.5.948rus . .

High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food

Radenović, Čedomir; Delić, Nenad; Radosavljević, Milica; Jovanović, Života; Sečanski, Mile; Popović, Aleksandar; Crevar, Miloš; Radosavljević, Nebojša

(Beograd : Univerzitet odbrane u Beogradu, Vojna akademija, 2021)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Delić, Nenad
AU  - Radosavljević, Milica
AU  - Jovanović, Života
AU  - Sečanski, Mile
AU  - Popović, Aleksandar
AU  - Crevar, Miloš
AU  - Radosavljević, Nebojša
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/826
AB  - Introduction/purpose: This paper presents the results of several different research studies. The inbred lines ZPPL 146 and ZPPL 159 and the maize hybrids ZP 633, ZP 735, and ZP 737 are primarily intended for human and livestock nutrition. Their selection took about four decades. Methods: Spectral bands were registered using the method of resonant Raman spectroscopy of the leaves of inbred maize lines. These spectral bands indicate the conformational characteristics of not only carotenoid molecules but also other compounds (phosphate, gluten, and amide III) in the leaf. Results: A systematic examination of the inbred lines ZPPL 146 and ZPPL 159 and their maize hybrids ZP 633, ZP 735, and ZP 737 was performed in this paper. It was stated that the new inbred lines of corn, i.e. ZPPL 146 and ZPPL 159, are rich in carotenoids and yellow pigments. These lines also have significant quantities of other valuable bioactive compounds and good physical characteristics. The lines have an upright position of the top leaves and belong to the group of maize lines with significant characteristics of the photosynthetic model. They are resistant to high temperatures and are drought tolerant. Conclusion: This paper presents the relevant properties, characteristics and parameters of the new studied inbred maize lines that can be used in selection processes in the future. High-yielding and high-quality maize hybrids, i.e. ZP 633, ZP 735, and ZP 737, have been created from the mentioned inbred maize lines. They are recognizable by their qualities. The hybrid ZP633 is especially noteworthy for human consumption (children and the elderly). Further, from the agronomic-veterinary point of view, it is confirmed that the hybrids ZP 735 and ZP 737 are the most suitable for livestock feeding with the programmed use of corn silage. The relevant agronomic, morphological and nutritional properties of the maize hybrids ZP 633, ZP 735, and ZP 737 are also presented in this paper. The results regarding the grain structure and yield height for grain and silage for the hybrids ZP 677 and ZP 684, produced in Serbia and the countries of Southeastern Europe, are also given.
PB  - Beograd : Univerzitet odbrane u Beogradu, Vojna akademija
T2  - Vojnotehnički glasnik
T1  - High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food
VL  - 69
IS  - 1
SP  - 114
EP  - 147
DO  - 10.5937/vojteh69-29512
ER  - 
@article{
author = "Radenović, Čedomir and Delić, Nenad and Radosavljević, Milica and Jovanović, Života and Sečanski, Mile and Popović, Aleksandar and Crevar, Miloš and Radosavljević, Nebojša",
year = "2021",
abstract = "Introduction/purpose: This paper presents the results of several different research studies. The inbred lines ZPPL 146 and ZPPL 159 and the maize hybrids ZP 633, ZP 735, and ZP 737 are primarily intended for human and livestock nutrition. Their selection took about four decades. Methods: Spectral bands were registered using the method of resonant Raman spectroscopy of the leaves of inbred maize lines. These spectral bands indicate the conformational characteristics of not only carotenoid molecules but also other compounds (phosphate, gluten, and amide III) in the leaf. Results: A systematic examination of the inbred lines ZPPL 146 and ZPPL 159 and their maize hybrids ZP 633, ZP 735, and ZP 737 was performed in this paper. It was stated that the new inbred lines of corn, i.e. ZPPL 146 and ZPPL 159, are rich in carotenoids and yellow pigments. These lines also have significant quantities of other valuable bioactive compounds and good physical characteristics. The lines have an upright position of the top leaves and belong to the group of maize lines with significant characteristics of the photosynthetic model. They are resistant to high temperatures and are drought tolerant. Conclusion: This paper presents the relevant properties, characteristics and parameters of the new studied inbred maize lines that can be used in selection processes in the future. High-yielding and high-quality maize hybrids, i.e. ZP 633, ZP 735, and ZP 737, have been created from the mentioned inbred maize lines. They are recognizable by their qualities. The hybrid ZP633 is especially noteworthy for human consumption (children and the elderly). Further, from the agronomic-veterinary point of view, it is confirmed that the hybrids ZP 735 and ZP 737 are the most suitable for livestock feeding with the programmed use of corn silage. The relevant agronomic, morphological and nutritional properties of the maize hybrids ZP 633, ZP 735, and ZP 737 are also presented in this paper. The results regarding the grain structure and yield height for grain and silage for the hybrids ZP 677 and ZP 684, produced in Serbia and the countries of Southeastern Europe, are also given.",
publisher = "Beograd : Univerzitet odbrane u Beogradu, Vojna akademija",
journal = "Vojnotehnički glasnik",
title = "High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food",
volume = "69",
number = "1",
pages = "114-147",
doi = "10.5937/vojteh69-29512"
}
Radenović, Č., Delić, N., Radosavljević, M., Jovanović, Ž., Sečanski, M., Popović, A., Crevar, M.,& Radosavljević, N.. (2021). High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food. in Vojnotehnički glasnik
Beograd : Univerzitet odbrane u Beogradu, Vojna akademija., 69(1), 114-147.
https://doi.org/10.5937/vojteh69-29512
Radenović Č, Delić N, Radosavljević M, Jovanović Ž, Sečanski M, Popović A, Crevar M, Radosavljević N. High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food. in Vojnotehnički glasnik. 2021;69(1):114-147.
doi:10.5937/vojteh69-29512 .
Radenović, Čedomir, Delić, Nenad, Radosavljević, Milica, Jovanović, Života, Sečanski, Mile, Popović, Aleksandar, Crevar, Miloš, Radosavljević, Nebojša, "High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food" in Vojnotehnički glasnik, 69, no. 1 (2021):114-147,
https://doi.org/10.5937/vojteh69-29512 . .

Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines

Božić, Manja; Nikolić, Ana; Marković, Ksenija; Delić, Nenad; Milivojević, Marija; Vančetović, Jelena; Mihai, Miritescu; Ignjatović-Micić, Dragana

(Juniper publishers inc., 2021)

TY  - JOUR
AU  - Božić, Manja
AU  - Nikolić, Ana
AU  - Marković, Ksenija
AU  - Delić, Nenad
AU  - Milivojević, Marija
AU  - Vančetović, Jelena
AU  - Mihai, Miritescu
AU  - Ignjatović-Micić, Dragana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1307
AB  - The consequences of the current climate changes sustain the strategy of early sowing (early spring) to avoid drought during the flowering
period and thus secure maize yields. Earlier sowing dates increase the risk of exposure of the plants to cold during the early development stages
and thus re-evaluation of elite material is required to select more tolerant genotypes. The first step of re-evaluation is to define characteristics that
could be used for fast survey of the elite material, before performing more profound analyses. The aim of this paper was to analyse morphological
characteristics (radicle length, coleoptile length, seedling weight) of 5 day old seedlings of two inbred lines LK and Mo17 (according to breeders
experience tolerant and susceptible, respectively) subjected to chilling stress (10°C day/8°C night) for 24h and 48h, as well as to perform
morphological (root and shoot characteristics) and physiological (chlorophyl, flavonoid and anthocyanin contents) analyses of the plants after
seven day recovery. Control plants were grown under optimal conditions (30°C day /20°C night, ≈60% humidity), at an 8h photoperiod. The
results were not straightforward, as the susceptible Mo17 showed faster recovery (statistically insignificant differences between control and
treatment plants for most of the analysed traits), while the tolerant LK was a more robust genotype throughout the experiment. Chlorophyl,
flavonoid and anthocyanin contents were not informative. The results identified survival rate and root characteristics as informative for fast
screening of seedlings for determination of cold tolerance.
PB  - Juniper publishers inc.
T2  - Agricultural research and technology
T1  - Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines
VL  - 25
IS  - 4
SP  - 5562311
DO  - 10.19080/ARTOAJ.2021.25.556311
ER  - 
@article{
author = "Božić, Manja and Nikolić, Ana and Marković, Ksenija and Delić, Nenad and Milivojević, Marija and Vančetović, Jelena and Mihai, Miritescu and Ignjatović-Micić, Dragana",
year = "2021",
abstract = "The consequences of the current climate changes sustain the strategy of early sowing (early spring) to avoid drought during the flowering
period and thus secure maize yields. Earlier sowing dates increase the risk of exposure of the plants to cold during the early development stages
and thus re-evaluation of elite material is required to select more tolerant genotypes. The first step of re-evaluation is to define characteristics that
could be used for fast survey of the elite material, before performing more profound analyses. The aim of this paper was to analyse morphological
characteristics (radicle length, coleoptile length, seedling weight) of 5 day old seedlings of two inbred lines LK and Mo17 (according to breeders
experience tolerant and susceptible, respectively) subjected to chilling stress (10°C day/8°C night) for 24h and 48h, as well as to perform
morphological (root and shoot characteristics) and physiological (chlorophyl, flavonoid and anthocyanin contents) analyses of the plants after
seven day recovery. Control plants were grown under optimal conditions (30°C day /20°C night, ≈60% humidity), at an 8h photoperiod. The
results were not straightforward, as the susceptible Mo17 showed faster recovery (statistically insignificant differences between control and
treatment plants for most of the analysed traits), while the tolerant LK was a more robust genotype throughout the experiment. Chlorophyl,
flavonoid and anthocyanin contents were not informative. The results identified survival rate and root characteristics as informative for fast
screening of seedlings for determination of cold tolerance.",
publisher = "Juniper publishers inc.",
journal = "Agricultural research and technology",
title = "Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines",
volume = "25",
number = "4",
pages = "5562311",
doi = "10.19080/ARTOAJ.2021.25.556311"
}
Božić, M., Nikolić, A., Marković, K., Delić, N., Milivojević, M., Vančetović, J., Mihai, M.,& Ignjatović-Micić, D.. (2021). Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines. in Agricultural research and technology
Juniper publishers inc.., 25(4), 5562311.
https://doi.org/10.19080/ARTOAJ.2021.25.556311
Božić M, Nikolić A, Marković K, Delić N, Milivojević M, Vančetović J, Mihai M, Ignjatović-Micić D. Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines. in Agricultural research and technology. 2021;25(4):5562311.
doi:10.19080/ARTOAJ.2021.25.556311 .
Božić, Manja, Nikolić, Ana, Marković, Ksenija, Delić, Nenad, Milivojević, Marija, Vančetović, Jelena, Mihai, Miritescu, Ignjatović-Micić, Dragana, "Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines" in Agricultural research and technology, 25, no. 4 (2021):5562311,
https://doi.org/10.19080/ARTOAJ.2021.25.556311 . .

Пастеризовани клипови младог кукуруза обогаћени антоцијанима изолованим из семењаче црне соје – Baby corn производ

Nikolić, Valentina; Simić, Marijana; Žilić, Slađana; Srdić, Jelena; Perić, Vesna; Pavlov, Jovan; Delić, Nenad

(2021)

TY  - GEN
AU  - Nikolić, Valentina
AU  - Simić, Marijana
AU  - Žilić, Slađana
AU  - Srdić, Jelena
AU  - Perić, Vesna
AU  - Pavlov, Jovan
AU  - Delić, Nenad
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1174
AB  - Овим техничким решењем описан је технолошки поступак добијања конзервисаног производа од младих клипова кукуруза baby corn обогаћеног антоцијанинима из семењаче црне соје. Добијен је стабилан функционални прехрамбени производ обогаћен биоактивним једињењима која могу позитивно утицати на здравље конзумената. Искоришћењем сојине семењаче – споредног производа из прераде соје додатно је валоризована ова сировина. Висок садржај прехрамбених влакана је такође посебно важан због неопходности повећаног уноса ових хранљивих материја у савременој исхрани због повољног утицаја на гастроинтестинално здравље. Сензорском анализом производ добијен од младих клипова хибрида кокичара са додатком 3% соли и 2% шећера такође је најбоље оцењен од стране панелиста на основу већег броја оцењиваних параметара (крцкавост, сјајност, интензитет боје, арома на ферментисано поврће). Киселост овог производа је означена као најприхватљивија (најмање кисео), што је раније потврђено и хемијском анализом pH вредности. На основу сумираних резултата, хибрид кокичар са наливом који садржи со (3%) и шећер (2%) оцењен је као оптималан за производњу пастеризованог производа од младих клипова кукуруза обогаћеног антоцијанинима екстрахованим из семењаче црне соје. Узимањем у обзир изузетно висок садржај укупних фенолних једињења, флавоноида, антоцијана, као и висок антиоксидативни капацитет, овај производ је одабран као оптималан за производњу на индустријском нивоу.
T2  - 53. redovna sednica Matičnog naučnog odbora za biotehnologiju i poljoprivredu, održanoj elektronskim putem 25.11.2021. godine
T1  - Пастеризовани клипови младог кукуруза обогаћени антоцијанима изолованим из семењаче црне соје – Baby corn производ
UR  - https://hdl.handle.net/21.15107/rcub_rik_1174
ER  - 
@misc{
author = "Nikolić, Valentina and Simić, Marijana and Žilić, Slađana and Srdić, Jelena and Perić, Vesna and Pavlov, Jovan and Delić, Nenad",
year = "2021",
abstract = "Овим техничким решењем описан је технолошки поступак добијања конзервисаног производа од младих клипова кукуруза baby corn обогаћеног антоцијанинима из семењаче црне соје. Добијен је стабилан функционални прехрамбени производ обогаћен биоактивним једињењима која могу позитивно утицати на здравље конзумената. Искоришћењем сојине семењаче – споредног производа из прераде соје додатно је валоризована ова сировина. Висок садржај прехрамбених влакана је такође посебно важан због неопходности повећаног уноса ових хранљивих материја у савременој исхрани због повољног утицаја на гастроинтестинално здравље. Сензорском анализом производ добијен од младих клипова хибрида кокичара са додатком 3% соли и 2% шећера такође је најбоље оцењен од стране панелиста на основу већег броја оцењиваних параметара (крцкавост, сјајност, интензитет боје, арома на ферментисано поврће). Киселост овог производа је означена као најприхватљивија (најмање кисео), што је раније потврђено и хемијском анализом pH вредности. На основу сумираних резултата, хибрид кокичар са наливом који садржи со (3%) и шећер (2%) оцењен је као оптималан за производњу пастеризованог производа од младих клипова кукуруза обогаћеног антоцијанинима екстрахованим из семењаче црне соје. Узимањем у обзир изузетно висок садржај укупних фенолних једињења, флавоноида, антоцијана, као и висок антиоксидативни капацитет, овај производ је одабран као оптималан за производњу на индустријском нивоу.",
journal = "53. redovna sednica Matičnog naučnog odbora za biotehnologiju i poljoprivredu, održanoj elektronskim putem 25.11.2021. godine",
title = "Пастеризовани клипови младог кукуруза обогаћени антоцијанима изолованим из семењаче црне соје – Baby corn производ",
url = "https://hdl.handle.net/21.15107/rcub_rik_1174"
}
Nikolić, V., Simić, M., Žilić, S., Srdić, J., Perić, V., Pavlov, J.,& Delić, N.. (2021). Пастеризовани клипови младог кукуруза обогаћени антоцијанима изолованим из семењаче црне соје – Baby corn производ. in 53. redovna sednica Matičnog naučnog odbora za biotehnologiju i poljoprivredu, održanoj elektronskim putem 25.11.2021. godine.
https://hdl.handle.net/21.15107/rcub_rik_1174
Nikolić V, Simić M, Žilić S, Srdić J, Perić V, Pavlov J, Delić N. Пастеризовани клипови младог кукуруза обогаћени антоцијанима изолованим из семењаче црне соје – Baby corn производ. in 53. redovna sednica Matičnog naučnog odbora za biotehnologiju i poljoprivredu, održanoj elektronskim putem 25.11.2021. godine. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1174 .
Nikolić, Valentina, Simić, Marijana, Žilić, Slađana, Srdić, Jelena, Perić, Vesna, Pavlov, Jovan, Delić, Nenad, "Пастеризовани клипови младог кукуруза обогаћени антоцијанима изолованим из семењаче црне соје – Baby corn производ" in 53. redovna sednica Matičnog naučnog odbora za biotehnologiju i poljoprivredu, održanoj elektronskim putem 25.11.2021. godine (2021),
https://hdl.handle.net/21.15107/rcub_rik_1174 .

Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Vasić, Marko G.; Srdić, Jelena; Delić, Nenad; Delić, Nenad

(Novi Sad : National Society of Processing and Energy in Agriculture, 2021)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Vasić, Marko G.
AU  - Srdić, Jelena
AU  - Delić, Nenad
AU  - Delić, Nenad
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/846
AB  - Five maize hybrids were used in this research: two yellow and one white dent, sweet hybrid, and yellow popcorn. Five brine
recipes with acetic acid were examined, of which two with the addition of potassium sorbate. The brines with preservatives were
stable for 16 months without colour changes of the liquid and corn cobs. The pH of brines ranged from 3.39 to 3.89. Canned hybrids
ZP 366 and ZP 611k in brine without sugar, and with the addition of potassium sorbate and potassium metabisulfite marked as
Number 5, showed the best sensory characteristics. The protein content determined in ZP 366 (9.56 %) and ZP 611k (10.23 %) did
not vary significantly compared to whole-grain maize flour, while crude fibre content (7.67 and 6.88 %), and ash content (21.96 and
20.72 %) were significantly higher than in flour (crude fibre: 2.40; 2.64 %, ash:1.35; 1.48 %, respectively). This research will be
continued in order to implement preliminary findings and new data on this subject
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane
T1  - Possibilities of maize hybrids utilization in canned baby corn production
VL  - 25
IS  - 1
SP  - 16
EP  - 19
DO  - 10.5937/jpea25-30887
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Vasić, Marko G. and Srdić, Jelena and Delić, Nenad and Delić, Nenad",
year = "2021",
abstract = "Five maize hybrids were used in this research: two yellow and one white dent, sweet hybrid, and yellow popcorn. Five brine
recipes with acetic acid were examined, of which two with the addition of potassium sorbate. The brines with preservatives were
stable for 16 months without colour changes of the liquid and corn cobs. The pH of brines ranged from 3.39 to 3.89. Canned hybrids
ZP 366 and ZP 611k in brine without sugar, and with the addition of potassium sorbate and potassium metabisulfite marked as
Number 5, showed the best sensory characteristics. The protein content determined in ZP 366 (9.56 %) and ZP 611k (10.23 %) did
not vary significantly compared to whole-grain maize flour, while crude fibre content (7.67 and 6.88 %), and ash content (21.96 and
20.72 %) were significantly higher than in flour (crude fibre: 2.40; 2.64 %, ash:1.35; 1.48 %, respectively). This research will be
continued in order to implement preliminary findings and new data on this subject",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane, Possibilities of maize hybrids utilization in canned baby corn production",
volume = "25",
number = "1",
pages = "16-19",
doi = "10.5937/jpea25-30887"
}
Nikolić, V., Žilić, S., Simić, M., Vasić, M. G., Srdić, J., Delić, N.,& Delić, N.. (2021). Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 25(1), 16-19.
https://doi.org/10.5937/jpea25-30887
Nikolić V, Žilić S, Simić M, Vasić MG, Srdić J, Delić N, Delić N. Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane. in Journal on Processing and Energy in Agriculture. 2021;25(1):16-19.
doi:10.5937/jpea25-30887 .
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Vasić, Marko G., Srdić, Jelena, Delić, Nenad, Delić, Nenad, "Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane" in Journal on Processing and Energy in Agriculture, 25, no. 1 (2021):16-19,
https://doi.org/10.5937/jpea25-30887 . .
2

Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature

Božić, Manja; Nikolić, Ana; Dudić, Dragana; Ignjatović-Micić, Dragana; Samardžić, Jelena; Delić, Nenad; Banović Đeri, Bojana

(Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, 2021)

TY  - CONF
AU  - Božić, Manja
AU  - Nikolić, Ana
AU  - Dudić, Dragana
AU  - Ignjatović-Micić, Dragana
AU  - Samardžić, Jelena
AU  - Delić, Nenad
AU  - Banović Đeri, Bojana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1147
AB  - Finding new ways of improving crop quality, yield potential and abiotic stress tolerance are some of the most important pursuits in crop production today. As one of the biggest causes of yield and productivity reduction is climate change, specifically increasing temperatures and drought during the summer, a large number of strategies is focussed on lessening their negative effects. Cropping pattern changes include earlier sowing (early spring), when the temperatures are lower, as one of the most promising escape strategies for avoiding high summer temperatures. Thus, development of cold tolerant maize lines became an important goal.
Comparative analysis of 46 maize inbred lines belonging to two different genetic backgrounds, one predominantly cold tolerante (marked as Non-Lancaster) and the other predominantly cold sensitive (marked as Lancaster) in the field, was done by whole transriptome sequencing and differential gene expression (DGE) analysis.  Plants were grown under optimal, greenhouse conditions and sampled after completing the V4 growth stage. Total RNA isolated from leaves of three plants per inbred line was used for cDNA library preparation by Illumina TruSeq Stranded RNA LT kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent kit, v2 (2 x 150bp). Data manipulation and analysis was performed using a custom-made bioinformatics pipeline that included high throughput sequence data quality control (using FastQC), removal of low quality reads (using Trimmomatic tool, version 0.32), transcriptome assembly and mapping (using Cufflinks, version 2.2.1), expression quantification (using CuffDiff) and DGE analysis (using BLAST2GO and GO analysis Toolkit and Database for Agricultural Community, agriGO v2).  
DGE analysis revealed 77 differentially expressed genes (DEGs) between the Lancaster and the Non-Lancaster group, 21 of which were  statistically supported for differential expression between the two groups and annotated as involved in abiotic stress responses in maize and other plant species. To test DEGs response to cold stress expression of a subset of seven DEGs in eight inbred lines (4 belonging to Lancaster and 4 belonging to Non-Lancaster genetic background) was analyzed under 24h long exposure to low temperatures (6/4° C, 12h photoperiod), with sampling being done 6h and 24h after beginning of the treatment, as well as after 48h of recovery. Six DEGs showed different expression regulation dependent on cold exposure duration and genetic background.  These findings imply differently regulated processes between the analysed Lancaster and Non-Lancaster inbred lines, contributing to their different cold response and adaptation, and will be further used for the development of cold tolerant hybrids.
PB  - Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts
T1  - Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_rik_1147
ER  - 
@conference{
author = "Božić, Manja and Nikolić, Ana and Dudić, Dragana and Ignjatović-Micić, Dragana and Samardžić, Jelena and Delić, Nenad and Banović Đeri, Bojana",
year = "2021",
abstract = "Finding new ways of improving crop quality, yield potential and abiotic stress tolerance are some of the most important pursuits in crop production today. As one of the biggest causes of yield and productivity reduction is climate change, specifically increasing temperatures and drought during the summer, a large number of strategies is focussed on lessening their negative effects. Cropping pattern changes include earlier sowing (early spring), when the temperatures are lower, as one of the most promising escape strategies for avoiding high summer temperatures. Thus, development of cold tolerant maize lines became an important goal.
Comparative analysis of 46 maize inbred lines belonging to two different genetic backgrounds, one predominantly cold tolerante (marked as Non-Lancaster) and the other predominantly cold sensitive (marked as Lancaster) in the field, was done by whole transriptome sequencing and differential gene expression (DGE) analysis.  Plants were grown under optimal, greenhouse conditions and sampled after completing the V4 growth stage. Total RNA isolated from leaves of three plants per inbred line was used for cDNA library preparation by Illumina TruSeq Stranded RNA LT kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent kit, v2 (2 x 150bp). Data manipulation and analysis was performed using a custom-made bioinformatics pipeline that included high throughput sequence data quality control (using FastQC), removal of low quality reads (using Trimmomatic tool, version 0.32), transcriptome assembly and mapping (using Cufflinks, version 2.2.1), expression quantification (using CuffDiff) and DGE analysis (using BLAST2GO and GO analysis Toolkit and Database for Agricultural Community, agriGO v2).  
DGE analysis revealed 77 differentially expressed genes (DEGs) between the Lancaster and the Non-Lancaster group, 21 of which were  statistically supported for differential expression between the two groups and annotated as involved in abiotic stress responses in maize and other plant species. To test DEGs response to cold stress expression of a subset of seven DEGs in eight inbred lines (4 belonging to Lancaster and 4 belonging to Non-Lancaster genetic background) was analyzed under 24h long exposure to low temperatures (6/4° C, 12h photoperiod), with sampling being done 6h and 24h after beginning of the treatment, as well as after 48h of recovery. Six DEGs showed different expression regulation dependent on cold exposure duration and genetic background.  These findings imply differently regulated processes between the analysed Lancaster and Non-Lancaster inbred lines, contributing to their different cold response and adaptation, and will be further used for the development of cold tolerant hybrids.",
publisher = "Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts",
title = "Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_rik_1147"
}
Božić, M., Nikolić, A., Dudić, D., Ignjatović-Micić, D., Samardžić, J., Delić, N.,& Banović Đeri, B.. (2021). Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature. in Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts
Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43.
https://hdl.handle.net/21.15107/rcub_rik_1147
Božić M, Nikolić A, Dudić D, Ignjatović-Micić D, Samardžić J, Delić N, Banović Đeri B. Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature. in Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts. 2021;43.
https://hdl.handle.net/21.15107/rcub_rik_1147 .
Božić, Manja, Nikolić, Ana, Dudić, Dragana, Ignjatović-Micić, Dragana, Samardžić, Jelena, Delić, Nenad, Banović Đeri, Bojana, "Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature" in Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts, 43 (2021),
https://hdl.handle.net/21.15107/rcub_rik_1147 .