Stanković, Goran

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orcid::0000-0002-7723-3238
  • Stanković, Goran (44)
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Author's Bibliography

ZP569

Stanković, Goran; Nikolić, Milica

(2021)

TY  - PAT
AU  - Stanković, Goran
AU  - Nikolić, Milica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1096
T2  - Rešenje:320-44-02156/2/2020-11
T1  - ZP569
ER  - 
@misc{
author = "Stanković, Goran and Nikolić, Milica",
year = "2021",
journal = "Rešenje:320-44-02156/2/2020-11",
title = "ZP569"
}
Stanković, G.,& Nikolić, M.. (2021). ZP569. in Rešenje:320-44-02156/2/2020-11.
Stanković G, Nikolić M. ZP569. in Rešenje:320-44-02156/2/2020-11. 2021;..
Stanković, Goran, Nikolić, Milica, "ZP569" in Rešenje:320-44-02156/2/2020-11 (2021).

ZP565

Stanković, Goran; Stanković, Slavica

(2021)

TY  - PAT
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1095
T2  - Rešenje:320-44-02152/2/2020-11
T1  - ZP565
ER  - 
@misc{
author = "Stanković, Goran and Stanković, Slavica",
year = "2021",
journal = "Rešenje:320-44-02152/2/2020-11",
title = "ZP565"
}
Stanković, G.,& Stanković, S.. (2021). ZP565. in Rešenje:320-44-02152/2/2020-11.
Stanković G, Stanković S. ZP565. in Rešenje:320-44-02152/2/2020-11. 2021;..
Stanković, Goran, Stanković, Slavica, "ZP565" in Rešenje:320-44-02152/2/2020-11 (2021).

ZP5252

Stevanović, Milan; Delić, Nenad; Filipović, Milomir; Stanković, Goran

(2021)

TY  - PAT
AU  - Stevanović, Milan
AU  - Delić, Nenad
AU  - Filipović, Milomir
AU  - Stanković, Goran
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1104
T2  - Rešenje:320-44-02165/2/2020-11
T1  - ZP5252
ER  - 
@misc{
author = "Stevanović, Milan and Delić, Nenad and Filipović, Milomir and Stanković, Goran",
year = "2021",
journal = "Rešenje:320-44-02165/2/2020-11",
title = "ZP5252"
}
Stevanović, M., Delić, N., Filipović, M.,& Stanković, G.. (2021). ZP5252. in Rešenje:320-44-02165/2/2020-11.
Stevanović M, Delić N, Filipović M, Stanković G. ZP5252. in Rešenje:320-44-02165/2/2020-11. 2021;..
Stevanović, Milan, Delić, Nenad, Filipović, Milomir, Stanković, Goran, "ZP5252" in Rešenje:320-44-02165/2/2020-11 (2021).

First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia

Obradović, Ana; Stepanović, Jelena; Krnjaja, Vesna; Bulajić, Aleksandra; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(St. Paul : APS publications, 2022)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stepanović, Jelena
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/853
PB  - St. Paul : APS publications
T2  - Plant Disease
T1  - First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia
VL  - 106
IS  - 2
SP  - 758
EP  - 758
DO  - 10.1094/PDIS-04-21-0715-PDN
ER  - 
@article{
author = "Obradović, Ana and Stepanović, Jelena and Krnjaja, Vesna and Bulajić, Aleksandra and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2022",
publisher = "St. Paul : APS publications",
journal = "Plant Disease",
title = "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia",
volume = "106",
number = "2",
pages = "758-758",
doi = "10.1094/PDIS-04-21-0715-PDN"
}
Obradović, A., Stepanović, J., Krnjaja, V., Bulajić, A., Stanković, G., Stevanović, M.,& Stanković, S.. (2022). First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease
St. Paul : APS publications., 106(2), 758-758.
https://doi.org/10.1094/PDIS-04-21-0715-PDN
Obradović A, Stepanović J, Krnjaja V, Bulajić A, Stanković G, Stevanović M, Stanković S. First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease. 2022;106(2):758-758.
doi:10.1094/PDIS-04-21-0715-PDN .
Obradović, Ana, Stepanović, Jelena, Krnjaja, Vesna, Bulajić, Aleksandra, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia" in Plant Disease, 106, no. 2 (2022):758-758,
https://doi.org/10.1094/PDIS-04-21-0715-PDN . .

ZP795

Stanković, Goran; Delić, Nenad

(2021)

TY  - PAT
AU  - Stanković, Goran
AU  - Delić, Nenad
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1090
T2  - Rešenje:320-04-02287/2/2019-11
T1  - ZP795
ER  - 
@misc{
author = "Stanković, Goran and Delić, Nenad",
year = "2021",
journal = "Rešenje:320-04-02287/2/2019-11",
title = "ZP795"
}
Stanković, G.,& Delić, N.. (2021). ZP795. in Rešenje:320-04-02287/2/2019-11.
Stanković G, Delić N. ZP795. in Rešenje:320-04-02287/2/2019-11. 2021;..
Stanković, Goran, Delić, Nenad, "ZP795" in Rešenje:320-04-02287/2/2019-11 (2021).

ZP711

Stanković, Goran

(2021)

TY  - PAT
AU  - Stanković, Goran
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1088
T2  - Rešenje:320-04-02289/2/2019-11
T1  - ZP711
ER  - 
@misc{
author = "Stanković, Goran",
year = "2021",
journal = "Rešenje:320-04-02289/2/2019-11",
title = "ZP711"
}
Stanković, G.. (2021). ZP711. in Rešenje:320-04-02289/2/2019-11.
Stanković G. ZP711. in Rešenje:320-04-02289/2/2019-11. 2021;..
Stanković, Goran, "ZP711" in Rešenje:320-04-02289/2/2019-11 (2021).

ZP710

Stanković, Goran; Filipović, Milomir; Obradović, Ana

(2021)

TY  - PAT
AU  - Stanković, Goran
AU  - Filipović, Milomir
AU  - Obradović, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1087
T2  - Rešenje:320-04-02279/2/2019-11
T1  - ZP710
ER  - 
@misc{
author = "Stanković, Goran and Filipović, Milomir and Obradović, Ana",
year = "2021",
journal = "Rešenje:320-04-02279/2/2019-11",
title = "ZP710"
}
Stanković, G., Filipović, M.,& Obradović, A.. (2021). ZP710. in Rešenje:320-04-02279/2/2019-11.
Stanković G, Filipović M, Obradović A. ZP710. in Rešenje:320-04-02279/2/2019-11. 2021;..
Stanković, Goran, Filipović, Milomir, Obradović, Ana, "ZP710" in Rešenje:320-04-02279/2/2019-11 (2021).

ZP568

Stanković, Goran; Filipović, Milomir; Obradović, Ana

(2021)

TY  - PAT
AU  - Stanković, Goran
AU  - Filipović, Milomir
AU  - Obradović, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1078
T2  - Rešenje:320-04-02281/2/2019-11
T1  - ZP568
ER  - 
@misc{
author = "Stanković, Goran and Filipović, Milomir and Obradović, Ana",
year = "2021",
journal = "Rešenje:320-04-02281/2/2019-11",
title = "ZP568"
}
Stanković, G., Filipović, M.,& Obradović, A.. (2021). ZP568. in Rešenje:320-04-02281/2/2019-11.
Stanković G, Filipović M, Obradović A. ZP568. in Rešenje:320-04-02281/2/2019-11. 2021;..
Stanković, Goran, Filipović, Milomir, Obradović, Ana, "ZP568" in Rešenje:320-04-02281/2/2019-11 (2021).

First report of Aspergillus parasiticus on Barley Grain in Serbia

Nikolić, Milica; Savić, Iva; Obradović, Ana; Srdić, Jelena; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(St. Paul : The American Phytopathological Society (APS), 2020)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Obradović, Ana
AU  - Srdić, Jelena
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/796
AB  - Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.
PB  - St. Paul : The American Phytopathological Society (APS)
T2  - Plant Disease
T1  - First report of Aspergillus parasiticus on Barley Grain in Serbia
VL  - 104
IS  - 3
SP  - 987
DO  - 10.1094/PDIS-07-19-1364-PDN
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Obradović, Ana and Srdić, Jelena and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2020",
abstract = "Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.",
publisher = "St. Paul : The American Phytopathological Society (APS)",
journal = "Plant Disease",
title = "First report of Aspergillus parasiticus on Barley Grain in Serbia",
volume = "104",
number = "3",
pages = "987",
doi = "10.1094/PDIS-07-19-1364-PDN"
}
Nikolić, M., Savić, I., Obradović, A., Srdić, J., Stanković, G., Stevanović, M.,& Stanković, S.. (2020). First report of Aspergillus parasiticus on Barley Grain in Serbia. in Plant Disease
St. Paul : The American Phytopathological Society (APS)., 104(3), 987.
https://doi.org/10.1094/PDIS-07-19-1364-PDN
Nikolić M, Savić I, Obradović A, Srdić J, Stanković G, Stevanović M, Stanković S. First report of Aspergillus parasiticus on Barley Grain in Serbia. in Plant Disease. 2020;104(3):987.
doi:10.1094/PDIS-07-19-1364-PDN .
Nikolić, Milica, Savić, Iva, Obradović, Ana, Srdić, Jelena, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First report of Aspergillus parasiticus on Barley Grain in Serbia" in Plant Disease, 104, no. 3 (2020):987,
https://doi.org/10.1094/PDIS-07-19-1364-PDN . .
3
1

Estimation of genetic diversity among maize inbred lines

Ristić, Danijela; Ignjatovic-Micic, Dragana; Mladenović Drinić, Snežana; Obradović, Ana; Kostadinović, Marija; Stevanović, Milan; Stanković, Goran

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2018)

TY  - CONF
AU  - Ristić, Danijela
AU  - Ignjatovic-Micic, Dragana
AU  - Mladenović Drinić, Snežana
AU  - Obradović, Ana
AU  - Kostadinović, Marija
AU  - Stevanović, Milan
AU  - Stanković, Goran
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/976
AB  - One of the most effective method in maize (Zea mays L) selection of adapted material is to
create synthetic populations from inbred lines of known origin and superior properties. The
methods used for inter- and intra-population synthetic improvement are some of the recurrent
selection techniques. Success of recurrent selection depends on the choice of parent
components and the method that will be used to obtain new inbred lines. The aim of this paper
was to apply molecular markers for estimation of genetic variability of inbred lines, as
potential parent components of synthetic populations. Molecular characterization of 26 inbred
lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total
number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036,
phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were
calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity
value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26)
was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three
groups mostly in accordance with their origin. The variability detected using SSR markers
could be useful in selecting best parental combinations in creating synthetic populations.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina
T1  - Estimation of genetic diversity among maize inbred lines
SP  - 280
EP  - 284
ER  - 
@conference{
author = "Ristić, Danijela and Ignjatovic-Micic, Dragana and Mladenović Drinić, Snežana and Obradović, Ana and Kostadinović, Marija and Stevanović, Milan and Stanković, Goran",
year = "2018",
abstract = "One of the most effective method in maize (Zea mays L) selection of adapted material is to
create synthetic populations from inbred lines of known origin and superior properties. The
methods used for inter- and intra-population synthetic improvement are some of the recurrent
selection techniques. Success of recurrent selection depends on the choice of parent
components and the method that will be used to obtain new inbred lines. The aim of this paper
was to apply molecular markers for estimation of genetic variability of inbred lines, as
potential parent components of synthetic populations. Molecular characterization of 26 inbred
lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total
number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036,
phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were
calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity
value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26)
was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three
groups mostly in accordance with their origin. The variability detected using SSR markers
could be useful in selecting best parental combinations in creating synthetic populations.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina",
title = "Estimation of genetic diversity among maize inbred lines",
pages = "280-284"
}
Ristić, D., Ignjatovic-Micic, D., Mladenović Drinić, S., Obradović, A., Kostadinović, M., Stevanović, M.,& Stanković, G.. (2018). Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 280-284.
Ristić D, Ignjatovic-Micic D, Mladenović Drinić S, Obradović A, Kostadinović M, Stevanović M, Stanković G. Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina. 2018;:280-284..
Ristić, Danijela, Ignjatovic-Micic, Dragana, Mladenović Drinić, Snežana, Obradović, Ana, Kostadinović, Marija, Stevanović, Milan, Stanković, Goran, "Estimation of genetic diversity among maize inbred lines" in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina (2018):280-284.

Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence

Obradović, Ana; Krnjaja, Vesna; Nikolić, Milica; Delibašić, Goran; Filipović, Milomir; Stanković, Goran; Stanković, Slavica

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Nikolić, Milica
AU  - Delibašić, Goran
AU  - Filipović, Milomir
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/738
AB  - Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species.
AB  - Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence
T1  - Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava
VL  - 34
IS  - 4
SP  - 469
EP  - 480
DO  - 10.2298/BAH1804469O
UR  - conv_2004
ER  - 
@article{
author = "Obradović, Ana and Krnjaja, Vesna and Nikolić, Milica and Delibašić, Goran and Filipović, Milomir and Stanković, Goran and Stanković, Slavica",
year = "2018",
abstract = "Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species., Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence, Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava",
volume = "34",
number = "4",
pages = "469-480",
doi = "10.2298/BAH1804469O",
url = "conv_2004"
}
Obradović, A., Krnjaja, V., Nikolić, M., Delibašić, G., Filipović, M., Stanković, G.,& Stanković, S.. (2018). Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(4), 469-480.
https://doi.org/10.2298/BAH1804469O
conv_2004
Obradović A, Krnjaja V, Nikolić M, Delibašić G, Filipović M, Stanković G, Stanković S. Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence. in Biotechnology in Animal Husbandry. 2018;34(4):469-480.
doi:10.2298/BAH1804469O
conv_2004 .
Obradović, Ana, Krnjaja, Vesna, Nikolić, Milica, Delibašić, Goran, Filipović, Milomir, Stanković, Goran, Stanković, Slavica, "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence" in Biotechnology in Animal Husbandry, 34, no. 4 (2018):469-480,
https://doi.org/10.2298/BAH1804469O .,
conv_2004 .
8

Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Stanković, Goran; Delić, Nenad

(Beograd : Društvo genetičara Srbije, 2018)

TY  - GEN
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Delić, Nenad
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/961
AB  - Molekularna genetika je danas sastavni deo različitih programa selekcije i oplemenjivanja žitarica u svetu. Najveći napredak u oplemenjivanju primenom molekularnih markera (eng. Marker Assisted Selection - MAS) postignut je kod kvalitativnih osobina koje su regulisane dejstvom jednog ili nekoliko gena i jasno su fenotipski definisane. Molekularni markeri se koriste kao selekcioni markeri za željeni gen (foreground selection), kao i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.
Jedan od primera uspešne primene MAS je povećanje hranljive vrednosti zrna kukuruza. Upotrebom molekularnih markera specifičnih za ciljne gene opaque2, crtRB1 i lpa2-2 dobijaju se linije kukuruza visokog kvaliteta proteina, visokog sadržaja beta karotena i niskog sadržaja fitina, redom. Dobijene linije koriste se za stvaranje agronomski superiornih hibrida povećane hranljive i biološke vrednosti.
U Institutu za kukuruz "Zemun Polje" razvijen je program stvaranja genotipova kukuruza visokog kvaliteta proteina (eng. Quality Protein Maize - QPM) adaptiranih na umereno klimatsko područje kombinovanim pristupom klasične selekcije i selekcije pomoću molekularnih markera. Jedna komercijalna linija prevedena je na QPM varijantu, korišćenjem tropske linije CML 144 kao donora poželjnih svojstava, u procesu koji se sastojao iz dva ciklusa povratnog ukrštanja i tri ciklusa samooplodnje. Ukrštanjem ove prevedene linije sa američkim QPM linijama dobijeni su hibridi poboljšanog kvaliteta proteina namenjeni ishrani tovnih pilića i prasića.
AB  - Molecular genetic technologies are implemented into modern grain breeding programs in the world. The greatest progress in marker assisted selection (MAS) has been achieved with the qualitative traits that are regulated by the action of a single or several genes and are clearly phenotypically defined. Markers can be used to either control the target gene (foreground selection) or to accelerate the reconstruction of the recurrent parent genotype (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection. 
One successful example of MAS application is the improvement of the maize nutritional value. Using specific molecular markers for desirable genes opaque2, crtRB1 and lpa2-2, maize lines with enhanced protein quality, enhanced β-carotene content and low phytic acid content are developed, respectively. These lines are being used to obtain high-yielding hybrids with improved nutritional benefit. 
Breeding program for developing quality protein maize (QPM) adapted to temperate regions through marker assisted selection is being conducted at Maize Research Institute Zemun Polje. One commercial inbred line was converted to its QPM counterpart, using tropical QPM line CML 144 as a donor line, after two generations of backcrossing and three generations of selfing. By crossing this converted line with American QPM lines, the hybrids with enhanced protein quality aimed for broiler and piglets feeding are obtained.
PB  - Beograd : Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
T2  - 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata
T1  - Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna
T1  - Marker assisted selection for improving protein quality of maize grain
SP  - 170
EP  - 171
ER  - 
@misc{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Stanković, Goran and Delić, Nenad",
year = "2018",
abstract = "Molekularna genetika je danas sastavni deo različitih programa selekcije i oplemenjivanja žitarica u svetu. Najveći napredak u oplemenjivanju primenom molekularnih markera (eng. Marker Assisted Selection - MAS) postignut je kod kvalitativnih osobina koje su regulisane dejstvom jednog ili nekoliko gena i jasno su fenotipski definisane. Molekularni markeri se koriste kao selekcioni markeri za željeni gen (foreground selection), kao i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.
Jedan od primera uspešne primene MAS je povećanje hranljive vrednosti zrna kukuruza. Upotrebom molekularnih markera specifičnih za ciljne gene opaque2, crtRB1 i lpa2-2 dobijaju se linije kukuruza visokog kvaliteta proteina, visokog sadržaja beta karotena i niskog sadržaja fitina, redom. Dobijene linije koriste se za stvaranje agronomski superiornih hibrida povećane hranljive i biološke vrednosti.
U Institutu za kukuruz "Zemun Polje" razvijen je program stvaranja genotipova kukuruza visokog kvaliteta proteina (eng. Quality Protein Maize - QPM) adaptiranih na umereno klimatsko područje kombinovanim pristupom klasične selekcije i selekcije pomoću molekularnih markera. Jedna komercijalna linija prevedena je na QPM varijantu, korišćenjem tropske linije CML 144 kao donora poželjnih svojstava, u procesu koji se sastojao iz dva ciklusa povratnog ukrštanja i tri ciklusa samooplodnje. Ukrštanjem ove prevedene linije sa američkim QPM linijama dobijeni su hibridi poboljšanog kvaliteta proteina namenjeni ishrani tovnih pilića i prasića., Molecular genetic technologies are implemented into modern grain breeding programs in the world. The greatest progress in marker assisted selection (MAS) has been achieved with the qualitative traits that are regulated by the action of a single or several genes and are clearly phenotypically defined. Markers can be used to either control the target gene (foreground selection) or to accelerate the reconstruction of the recurrent parent genotype (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection. 
One successful example of MAS application is the improvement of the maize nutritional value. Using specific molecular markers for desirable genes opaque2, crtRB1 and lpa2-2, maize lines with enhanced protein quality, enhanced β-carotene content and low phytic acid content are developed, respectively. These lines are being used to obtain high-yielding hybrids with improved nutritional benefit. 
Breeding program for developing quality protein maize (QPM) adapted to temperate regions through marker assisted selection is being conducted at Maize Research Institute Zemun Polje. One commercial inbred line was converted to its QPM counterpart, using tropical QPM line CML 144 as a donor line, after two generations of backcrossing and three generations of selfing. By crossing this converted line with American QPM lines, the hybrids with enhanced protein quality aimed for broiler and piglets feeding are obtained.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata",
title = "Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna, Marker assisted selection for improving protein quality of maize grain",
pages = "170-171"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Stanković, G.,& Delić, N.. (2018). Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna. in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata
Beograd : Društvo genetičara Srbije., 170-171.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Stanković G, Delić N. Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna. in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata. 2018;:170-171..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Stanković, Goran, Delić, Nenad, "Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna" in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata (2018):170-171.

Assessment stability of maize lines yield by GGE-biplot analysis

Božović, Dragan; Zivanović, Tomislav; Popović, Vera; Tatić, Mladen; Gospavić, Zagorka; Miloradović, Zoran; Dokić, Milorad; Stanković, Goran

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Božović, Dragan
AU  - Zivanović, Tomislav
AU  - Popović, Vera
AU  - Tatić, Mladen
AU  - Gospavić, Zagorka
AU  - Miloradović, Zoran
AU  - Dokić, Milorad
AU  - Stanković, Goran
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/706
AB  - Maize genotypes have varied reactions in different localities, years, treatments or the combination of these factors, due to genotype x year interaction. The objective of this study was to estimate genotype by locality, by year, by treatments (GxLxYxT) interaction using AMMI model, to identify maize genotypes with stable and high yield performance in different growing seasons. The trials with seven maize lines/genotypes were conducted during two years (2010-2011) at the four treatments and two locations: Pancevo and Zemun Polje. The results showed that the influence of: genotype (G), locality (L), treatment (T) and GxL, GxT, YxL, YxT, LXT, GxYxT, GxLxT, YxLxT, GxYxLxT interaction, on maize yield were significant (p lt 0.01). The share of genotype for maize grain yield in the total phenotypic variance was 21.16%, the aggregate share of the years and the locality was 6.10%, the treatment was 18.22%, and the total interaction was 54.52%. The AMMI analysis of the main components of IPCA1 and IPCA2 for the interaction of GxL and GxT shows that the first major component, IPCA1, comprises 100% of the sum of the squared interaction GxL and showed a statistically significant effect. The results also show that the sums of the squares of the first and second major components (PC1 and PC2) constitute 100% of the sum of the squared interaction GxL. The IPCA1 share in the GxT interaction was 47.39% and the IPC2 was 37.94%. IPC1 and IPC2 for this interaction was 85.33%. A high level of IPC2 indicates a significant treatment effect. The results of AMI analysis show that there is a significant difference between the genotype reactions to different ecological conditions for investigated factors. It also provided better insight in specific association between maize grain yield, locality, treatment and meteorological variables. Among the tested maize lines/genotypes, L-5, L-4 and L-6 could be separated as highest yielding genotypes, however L-5 could be recommended for further breeding program and in large-scale seed production due to its stable and high yielding performance.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Assessment stability of maize lines yield by GGE-biplot analysis
VL  - 50
IS  - 3
SP  - 755
EP  - 770
DO  - 10.2298/GENSR1803755B
UR  - conv_1001
ER  - 
@article{
author = "Božović, Dragan and Zivanović, Tomislav and Popović, Vera and Tatić, Mladen and Gospavić, Zagorka and Miloradović, Zoran and Dokić, Milorad and Stanković, Goran",
year = "2018",
abstract = "Maize genotypes have varied reactions in different localities, years, treatments or the combination of these factors, due to genotype x year interaction. The objective of this study was to estimate genotype by locality, by year, by treatments (GxLxYxT) interaction using AMMI model, to identify maize genotypes with stable and high yield performance in different growing seasons. The trials with seven maize lines/genotypes were conducted during two years (2010-2011) at the four treatments and two locations: Pancevo and Zemun Polje. The results showed that the influence of: genotype (G), locality (L), treatment (T) and GxL, GxT, YxL, YxT, LXT, GxYxT, GxLxT, YxLxT, GxYxLxT interaction, on maize yield were significant (p lt 0.01). The share of genotype for maize grain yield in the total phenotypic variance was 21.16%, the aggregate share of the years and the locality was 6.10%, the treatment was 18.22%, and the total interaction was 54.52%. The AMMI analysis of the main components of IPCA1 and IPCA2 for the interaction of GxL and GxT shows that the first major component, IPCA1, comprises 100% of the sum of the squared interaction GxL and showed a statistically significant effect. The results also show that the sums of the squares of the first and second major components (PC1 and PC2) constitute 100% of the sum of the squared interaction GxL. The IPCA1 share in the GxT interaction was 47.39% and the IPC2 was 37.94%. IPC1 and IPC2 for this interaction was 85.33%. A high level of IPC2 indicates a significant treatment effect. The results of AMI analysis show that there is a significant difference between the genotype reactions to different ecological conditions for investigated factors. It also provided better insight in specific association between maize grain yield, locality, treatment and meteorological variables. Among the tested maize lines/genotypes, L-5, L-4 and L-6 could be separated as highest yielding genotypes, however L-5 could be recommended for further breeding program and in large-scale seed production due to its stable and high yielding performance.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Assessment stability of maize lines yield by GGE-biplot analysis",
volume = "50",
number = "3",
pages = "755-770",
doi = "10.2298/GENSR1803755B",
url = "conv_1001"
}
Božović, D., Zivanović, T., Popović, V., Tatić, M., Gospavić, Z., Miloradović, Z., Dokić, M.,& Stanković, G.. (2018). Assessment stability of maize lines yield by GGE-biplot analysis. in Genetika
Društvo genetičara Srbije, Beograd., 50(3), 755-770.
https://doi.org/10.2298/GENSR1803755B
conv_1001
Božović D, Zivanović T, Popović V, Tatić M, Gospavić Z, Miloradović Z, Dokić M, Stanković G. Assessment stability of maize lines yield by GGE-biplot analysis. in Genetika. 2018;50(3):755-770.
doi:10.2298/GENSR1803755B
conv_1001 .
Božović, Dragan, Zivanović, Tomislav, Popović, Vera, Tatić, Mladen, Gospavić, Zagorka, Miloradović, Zoran, Dokić, Milorad, Stanković, Goran, "Assessment stability of maize lines yield by GGE-biplot analysis" in Genetika, 50, no. 3 (2018):755-770,
https://doi.org/10.2298/GENSR1803755B .,
conv_1001 .
12
7
11

Comparison of statistical methods for genetic similarity evaluation of maize inbred lines

Nikolić, Ana; Kostadinović, Marija; Vančetović, Jelena; Stanković, Goran; Ignjatović-Micić, Dragana

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2017)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Ignjatović-Micić, Dragana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/679
AB  - Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index.
AB  - Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Comparison of statistical methods for genetic similarity evaluation of maize inbred lines
T1  - Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza
VL  - 54
IS  - 1
SP  - 25
EP  - 30
DO  - 10.5937/ratpov54-12174
UR  - conv_623
ER  - 
@article{
author = "Nikolić, Ana and Kostadinović, Marija and Vančetović, Jelena and Stanković, Goran and Ignjatović-Micić, Dragana",
year = "2017",
abstract = "Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index., Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines, Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza",
volume = "54",
number = "1",
pages = "25-30",
doi = "10.5937/ratpov54-12174",
url = "conv_623"
}
Nikolić, A., Kostadinović, M., Vančetović, J., Stanković, G.,& Ignjatović-Micić, D.. (2017). Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 54(1), 25-30.
https://doi.org/10.5937/ratpov54-12174
conv_623
Nikolić A, Kostadinović M, Vančetović J, Stanković G, Ignjatović-Micić D. Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo. 2017;54(1):25-30.
doi:10.5937/ratpov54-12174
conv_623 .
Nikolić, Ana, Kostadinović, Marija, Vančetović, Jelena, Stanković, Goran, Ignjatović-Micić, Dragana, "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines" in Ratarstvo i povrtarstvo, 54, no. 1 (2017):25-30,
https://doi.org/10.5937/ratpov54-12174 .,
conv_623 .
1

Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits

Kostadinović, Marija; Ignjatović-Micić, Dragana; Ristić, Danijela; Mladenović Drinić, Snežana; Vančetović, Jelena; Božinović, Sofija; Stanković, Goran

(Public Library Science, San Francisco, 2016)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Mladenović Drinić, Snežana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Stanković, Goran
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/621
AB  - Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.
PB  - Public Library Science, San Francisco
T2  - PLOS ONE
T1  - Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167635
UR  - conv_941
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Ristić, Danijela and Mladenović Drinić, Snežana and Vančetović, Jelena and Božinović, Sofija and Stanković, Goran",
year = "2016",
abstract = "Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.",
publisher = "Public Library Science, San Francisco",
journal = "PLOS ONE",
title = "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167635",
url = "conv_941"
}
Kostadinović, M., Ignjatović-Micić, D., Ristić, D., Mladenović Drinić, S., Vančetović, J., Božinović, S.,& Stanković, G.. (2016). Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167635
conv_941
Kostadinović M, Ignjatović-Micić D, Ristić D, Mladenović Drinić S, Vančetović J, Božinović S, Stanković G. Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE. 2016;11(12).
doi:10.1371/journal.pone.0167635
conv_941 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Ristić, Danijela, Mladenović Drinić, Snežana, Vančetović, Jelena, Božinović, Sofija, Stanković, Goran, "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits" in PLOS ONE, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167635 .,
conv_941 .
1
8
7
9

Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima

Stanković, Goran

(Универзитет у Београду, Пољопривредни факултет, 2016)

TY  - THES
AU  - Stanković, Goran
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=3772
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:12683/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48149519
UR  - http://nardus.mpn.gov.rs/123456789/6426
UR  - http://rik.mrizp.rs/handle/123456789/761
AB  - U radu je proučavana divergentnost sintetičkih populacija, roditeljskih linija i linija potomstava analizom srednjih vrednosti njihovih deset osobina i primenom SSR markera. Za istraţivanja su korišćene tri sintetičke populacije per se i njihove S1 familije, deset roditeljskih linija i jedanaest linija potomstava. Sintetička populacija Syn ZP je nastala dialelnim ukrštanjem devet inbred linija poreklom iz lokalnih sorti sa Zapadnog Balkana, Syn ZP/USA iz ukrštanja linija lokalnog porekla sa linijom poreklom iz BSS sintetika, a Syn FS rekombinacijom predhodne dve populacije. Linije potomstva su poreklom iz sintetičkih populacija Syn ZP i Syn ZP/USA.Na osnovu rezultata istraţivanja utvrĎeno je da su se S1 familije tri sintetičke populacije značajno razlikovale po srednjim vrednostima ispitivanih osobina. Varijabilnost i heritabilnost ispitivanih osobina je povećana nakon unošenja strane germplazme, kao i nakon rekombinacije sintetičkih populacija, a naročito je izraţeno povećanje genetičke dobiti od selekcije. Genetički koeficijenti korelacija su pokazali da je kod sve tri populacije najveći uticaj na prinos zrna imala duţina klipa, a najmanji masa 1000 zrna. Rezultati analize glavnih komponenti su pokazali da je duţina klipa, zajedno sa brojem zrna u redu, imala najveći indirektan uticaj na prinos zrna kod S1 familija sve tri sintetičke populacije. Inkorporacija strane germplazme, kao i rekombinacija sintetičkih populacija, su doprinele stvaranju nove varijabilnosti ispitivanog genetičkog materijala i poboljšanju srednjih vrednosti ispitivanih osobina.Rezultati istraţivanja sintetičkih populacija per se, roditeljskih komponenti i linija potomstava su ukazali da su selekcionisane linije imale bolje srednje vrednosti za ispitivane osobine od roditeljskih linija. Koeficijenti korelacija su bili pribliţno isti kod linija roditelja i linija potomstava, s tim da je uočeno povećanje uticaja mase zrna naprinos, u odnosu na S1 familije. Na osnovu ovih rezultata odabrano je nekoliko linija potomstava kod kojih je zabeleţeno poboljšanje osobina u odnosu na roditelje i koje mogu biti perspektivne za dalju selekciju...
AB  - Divergence of synthetic populations, parental and progeny inbred lines has been studied using both mean values of ten phenotypic traits and SSR markers. The research was performed on three synthetic populations per se and their S1 families, ten parental and 11 progeny inbred lines. Syn ZP synthetic population was obtained by diallel crossing of nine inbreds that originated from local, West Balkans, landraces, Syn ZP/USA by crossing inbreds of local origin with an inbred from BSS synthetic and Syn FS by recombination of the two previous populations. Progeny inbred lines were obtained from Syn ZP and Syn ZP/USA.The results revealed that S1 families of the three synthetics were significantly different in the mean values of the analysed traits. Variability and heritability of these traits has been increased after introduction of exotic germplasm as well as after recombination of the synthetic populations and genetic gain was particularly expressed. Genetic correlation coefficients showed that ear length had the highest and 1000 kernel weight the lowest impact on grain yield. Principal component analysis showed that ear length, together with kernel number per row, had the highest indirect impact on grain yield of S1 families considering all three synthetic populations. Incorporation of exotic germplasm, as well as recombination of the synthetic populations, contributed to the generation of new variability in the genetic material and improvement of mean values of the analysed traits.Analyses of synthetic populations per se, parental components and progeny inbreds indicated that new inbred lines had better mean values of the traits compared to parental inbreds. Correlation coefficients were approximately the same within parental inbreds and within progeny inbreds, except that increase of kernel weight impact ongrain yield in regard to S1 families was observed. Based on the presented results of the research, several progeny inbreds have been chosen, which have better trait performances in comparison to parents and could be perspective for further selection...
PB  - Универзитет у Београду, Пољопривредни факултет
T2  - Универзитет у Београду
T1  - Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima
T1  - Divergence of maize synthetic populations and their progenies determined by phenotypic and molecular markers
UR  - https://hdl.handle.net/21.15107/rcub_nardus_6426
ER  - 
@phdthesis{
author = "Stanković, Goran",
year = "2016",
abstract = "U radu je proučavana divergentnost sintetičkih populacija, roditeljskih linija i linija potomstava analizom srednjih vrednosti njihovih deset osobina i primenom SSR markera. Za istraţivanja su korišćene tri sintetičke populacije per se i njihove S1 familije, deset roditeljskih linija i jedanaest linija potomstava. Sintetička populacija Syn ZP je nastala dialelnim ukrštanjem devet inbred linija poreklom iz lokalnih sorti sa Zapadnog Balkana, Syn ZP/USA iz ukrštanja linija lokalnog porekla sa linijom poreklom iz BSS sintetika, a Syn FS rekombinacijom predhodne dve populacije. Linije potomstva su poreklom iz sintetičkih populacija Syn ZP i Syn ZP/USA.Na osnovu rezultata istraţivanja utvrĎeno je da su se S1 familije tri sintetičke populacije značajno razlikovale po srednjim vrednostima ispitivanih osobina. Varijabilnost i heritabilnost ispitivanih osobina je povećana nakon unošenja strane germplazme, kao i nakon rekombinacije sintetičkih populacija, a naročito je izraţeno povećanje genetičke dobiti od selekcije. Genetički koeficijenti korelacija su pokazali da je kod sve tri populacije najveći uticaj na prinos zrna imala duţina klipa, a najmanji masa 1000 zrna. Rezultati analize glavnih komponenti su pokazali da je duţina klipa, zajedno sa brojem zrna u redu, imala najveći indirektan uticaj na prinos zrna kod S1 familija sve tri sintetičke populacije. Inkorporacija strane germplazme, kao i rekombinacija sintetičkih populacija, su doprinele stvaranju nove varijabilnosti ispitivanog genetičkog materijala i poboljšanju srednjih vrednosti ispitivanih osobina.Rezultati istraţivanja sintetičkih populacija per se, roditeljskih komponenti i linija potomstava su ukazali da su selekcionisane linije imale bolje srednje vrednosti za ispitivane osobine od roditeljskih linija. Koeficijenti korelacija su bili pribliţno isti kod linija roditelja i linija potomstava, s tim da je uočeno povećanje uticaja mase zrna naprinos, u odnosu na S1 familije. Na osnovu ovih rezultata odabrano je nekoliko linija potomstava kod kojih je zabeleţeno poboljšanje osobina u odnosu na roditelje i koje mogu biti perspektivne za dalju selekciju..., Divergence of synthetic populations, parental and progeny inbred lines has been studied using both mean values of ten phenotypic traits and SSR markers. The research was performed on three synthetic populations per se and their S1 families, ten parental and 11 progeny inbred lines. Syn ZP synthetic population was obtained by diallel crossing of nine inbreds that originated from local, West Balkans, landraces, Syn ZP/USA by crossing inbreds of local origin with an inbred from BSS synthetic and Syn FS by recombination of the two previous populations. Progeny inbred lines were obtained from Syn ZP and Syn ZP/USA.The results revealed that S1 families of the three synthetics were significantly different in the mean values of the analysed traits. Variability and heritability of these traits has been increased after introduction of exotic germplasm as well as after recombination of the synthetic populations and genetic gain was particularly expressed. Genetic correlation coefficients showed that ear length had the highest and 1000 kernel weight the lowest impact on grain yield. Principal component analysis showed that ear length, together with kernel number per row, had the highest indirect impact on grain yield of S1 families considering all three synthetic populations. Incorporation of exotic germplasm, as well as recombination of the synthetic populations, contributed to the generation of new variability in the genetic material and improvement of mean values of the analysed traits.Analyses of synthetic populations per se, parental components and progeny inbreds indicated that new inbred lines had better mean values of the traits compared to parental inbreds. Correlation coefficients were approximately the same within parental inbreds and within progeny inbreds, except that increase of kernel weight impact ongrain yield in regard to S1 families was observed. Based on the presented results of the research, several progeny inbreds have been chosen, which have better trait performances in comparison to parents and could be perspective for further selection...",
publisher = "Универзитет у Београду, Пољопривредни факултет",
journal = "Универзитет у Београду",
title = "Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima, Divergence of maize synthetic populations and their progenies determined by phenotypic and molecular markers",
url = "https://hdl.handle.net/21.15107/rcub_nardus_6426"
}
Stanković, G.. (2016). Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima. in Универзитет у Београду
Универзитет у Београду, Пољопривредни факултет..
https://hdl.handle.net/21.15107/rcub_nardus_6426
Stanković G. Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima. in Универзитет у Београду. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_6426 .
Stanković, Goran, "Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima" in Универзитет у Београду (2016),
https://hdl.handle.net/21.15107/rcub_nardus_6426 .

Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value

Radenović, Čedomir; Delić, Nenad; Sečanski, Mile; Jovanović, Života; Stanković, Goran; Popović, Aleksandar

(2015)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Delić, Nenad
AU  - Sečanski, Mile
AU  - Jovanović, Života
AU  - Stanković, Goran
AU  - Popović, Aleksandar
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/601
AB  - This paper presents results of several different studies that confirm the hypothesis that maize inbred lines rich in proteins and with exceptional nutritive values can be bred. This is also supported by a medicinal standpoint of programmed need for maize-based food and feed. With such an experimental approach, the maize inbred lines ZPPL 146 and ZPPL 159 and hybrids derived from them (ZP 633, ZP 735 and ZP 737) rich in proteins have been systematically tested. Based on concrete results the following we can be conclude as follows. Selected maize inbred lines ZPPL 146 and ZPPL 159, rich in carotenoids, yellow pigments, also have significant amounts of other relevant bioactive compounds. Observed inbred lines have erect top leaves and are classified into a group of maize inbreds with significant properties of the photosynthesis model, are resistant to high temperatures and tolerant to drought. Spectral bands pointing to conformational characteristics of molecules of carotenoids but also of other compounds (phosphates, glutens and amides III) were established by the resonance Raman spectroscopy method applied to the leaf of the maize inbred line ZPLL 146. Physiological, biochemical and biophysical traits of elite maize inbred lines and their hybrids were observed in this study with a special emphasis on their efficiency of photosynthesis, productivity and suitability for broad use of nutrient values of grain and other essential biogenic matters, first of all pigments that express antioxidative properties. Relevant traits, properties and parameters of observed maize inbred lines that can be used in the process of selection are presented. These prestigious maize inbred lines were used to develop high-yielding and high-quality maize hybrids (ZP 633, ZP 735 and ZP 737) that are recognisable for their quality in human nutrition (children and the elderly), that are confirmed by medical observations related to their use in food and feed. Relevant agronomic and morphological traits of maize hybrids are presented. Moreover, results on grain structure and yields obtained in the regions of south-eastern Europe are also displayed.
T2  - Sel'skokhozyaistvennaya Biologiya
T1  - Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value
VL  - 50
IS  - 5
SP  - 600
EP  - 610
DO  - 10.15389/agrobiology.2015.5.600eng
UR  - conv_1022
ER  - 
@article{
author = "Radenović, Čedomir and Delić, Nenad and Sečanski, Mile and Jovanović, Života and Stanković, Goran and Popović, Aleksandar",
year = "2015",
abstract = "This paper presents results of several different studies that confirm the hypothesis that maize inbred lines rich in proteins and with exceptional nutritive values can be bred. This is also supported by a medicinal standpoint of programmed need for maize-based food and feed. With such an experimental approach, the maize inbred lines ZPPL 146 and ZPPL 159 and hybrids derived from them (ZP 633, ZP 735 and ZP 737) rich in proteins have been systematically tested. Based on concrete results the following we can be conclude as follows. Selected maize inbred lines ZPPL 146 and ZPPL 159, rich in carotenoids, yellow pigments, also have significant amounts of other relevant bioactive compounds. Observed inbred lines have erect top leaves and are classified into a group of maize inbreds with significant properties of the photosynthesis model, are resistant to high temperatures and tolerant to drought. Spectral bands pointing to conformational characteristics of molecules of carotenoids but also of other compounds (phosphates, glutens and amides III) were established by the resonance Raman spectroscopy method applied to the leaf of the maize inbred line ZPLL 146. Physiological, biochemical and biophysical traits of elite maize inbred lines and their hybrids were observed in this study with a special emphasis on their efficiency of photosynthesis, productivity and suitability for broad use of nutrient values of grain and other essential biogenic matters, first of all pigments that express antioxidative properties. Relevant traits, properties and parameters of observed maize inbred lines that can be used in the process of selection are presented. These prestigious maize inbred lines were used to develop high-yielding and high-quality maize hybrids (ZP 633, ZP 735 and ZP 737) that are recognisable for their quality in human nutrition (children and the elderly), that are confirmed by medical observations related to their use in food and feed. Relevant agronomic and morphological traits of maize hybrids are presented. Moreover, results on grain structure and yields obtained in the regions of south-eastern Europe are also displayed.",
journal = "Sel'skokhozyaistvennaya Biologiya",
title = "Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value",
volume = "50",
number = "5",
pages = "600-610",
doi = "10.15389/agrobiology.2015.5.600eng",
url = "conv_1022"
}
Radenović, Č., Delić, N., Sečanski, M., Jovanović, Ž., Stanković, G.,& Popović, A.. (2015). Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value. in Sel'skokhozyaistvennaya Biologiya, 50(5), 600-610.
https://doi.org/10.15389/agrobiology.2015.5.600eng
conv_1022
Radenović Č, Delić N, Sečanski M, Jovanović Ž, Stanković G, Popović A. Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value. in Sel'skokhozyaistvennaya Biologiya. 2015;50(5):600-610.
doi:10.15389/agrobiology.2015.5.600eng
conv_1022 .
Radenović, Čedomir, Delić, Nenad, Sečanski, Mile, Jovanović, Života, Stanković, Goran, Popović, Aleksandar, "Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value" in Sel'skokhozyaistvennaya Biologiya, 50, no. 5 (2015):600-610,
https://doi.org/10.15389/agrobiology.2015.5.600eng .,
conv_1022 .
1
1

Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line

Kostadinović, Marija; Ignjatović-Micić, Dragana; Stanković, Goran; Vančetović, Jelena; Ristić, Danijela; Mladenović Drinić, Snežana

(Burgas : Sciences events, 2014)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/959
AB  - Quality protein maize (QPM) is the maize with increased levels of two essential amino acids, hard endosperm and good agronomic performances. The recessive opaque2 (o2) mutation increases lysine and tryptophan content in maize grains. It has recently become possible to use marker assisted selection (MAS) to accelerate selection for the o2 allele in QPM breeding process. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The results presented herein are the part of marker assisted backcross breeding program for incorporation of the o2 into maize inbred line. Marker selected o2 recessive homozygous BC2F2 individuals were subjected to the whole genome background selection, which identified five progenies with 83 to 94% recurrent parent genome content. BC2F3 families are being evaluated for desirable agronomic and biochemical traits in replicated trials and the best lines will represent the QPM version of the standard line.
PB  - Burgas : Sciences events
C3  - Journal of international scientific publications: agriculture & food
T1  - Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line
VL  - 2
SP  - 199
EP  - 205
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Vančetović, Jelena and Ristić, Danijela and Mladenović Drinić, Snežana",
year = "2014",
abstract = "Quality protein maize (QPM) is the maize with increased levels of two essential amino acids, hard endosperm and good agronomic performances. The recessive opaque2 (o2) mutation increases lysine and tryptophan content in maize grains. It has recently become possible to use marker assisted selection (MAS) to accelerate selection for the o2 allele in QPM breeding process. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The results presented herein are the part of marker assisted backcross breeding program for incorporation of the o2 into maize inbred line. Marker selected o2 recessive homozygous BC2F2 individuals were subjected to the whole genome background selection, which identified five progenies with 83 to 94% recurrent parent genome content. BC2F3 families are being evaluated for desirable agronomic and biochemical traits in replicated trials and the best lines will represent the QPM version of the standard line.",
publisher = "Burgas : Sciences events",
journal = "Journal of international scientific publications: agriculture & food",
title = "Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line",
volume = "2",
pages = "199-205"
}
Kostadinović, M., Ignjatović-Micić, D., Stanković, G., Vančetović, J., Ristić, D.,& Mladenović Drinić, S.. (2014). Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line. in Journal of international scientific publications: agriculture & food
Burgas : Sciences events., 2, 199-205.
Kostadinović M, Ignjatović-Micić D, Stanković G, Vančetović J, Ristić D, Mladenović Drinić S. Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line. in Journal of international scientific publications: agriculture & food. 2014;2:199-205..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Vančetović, Jelena, Ristić, Danijela, Mladenović Drinić, Snežana, "Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line" in Journal of international scientific publications: agriculture & food, 2 (2014):199-205.

Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize

Kostadinović, Marija; Ignjatović-Micić, Dragana; Stanković, Goran; Vančetović, Jelena; Ristić, Danijela; Mladenović Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2014)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/526
AB  - Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize
VL  - 46
IS  - 2
SP  - 579
EP  - 590
DO  - 10.2298/GENSR1402579K
UR  - conv_888
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Vančetović, Jelena and Ristić, Danijela and Mladenović Drinić, Snežana",
year = "2014",
abstract = "Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize",
volume = "46",
number = "2",
pages = "579-590",
doi = "10.2298/GENSR1402579K",
url = "conv_888"
}
Kostadinović, M., Ignjatović-Micić, D., Stanković, G., Vančetović, J., Ristić, D.,& Mladenović Drinić, S.. (2014). Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika
Društvo genetičara Srbije, Beograd., 46(2), 579-590.
https://doi.org/10.2298/GENSR1402579K
conv_888
Kostadinović M, Ignjatović-Micić D, Stanković G, Vančetović J, Ristić D, Mladenović Drinić S. Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika. 2014;46(2):579-590.
doi:10.2298/GENSR1402579K
conv_888 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Vančetović, Jelena, Ristić, Danijela, Mladenović Drinić, Snežana, "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize" in Genetika, 46, no. 2 (2014):579-590,
https://doi.org/10.2298/GENSR1402579K .,
conv_888 .
2
1
2

Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence

Radenović, Čedomir; Stanković, Goran; Jovanović, Života; Maksimov, Georgij; Tyutyaev, Evgenij V.; Beljanski, Miloš

(Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux, 2014)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Stanković, Goran
AU  - Jovanović, Života
AU  - Maksimov, Georgij
AU  - Tyutyaev, Evgenij V.
AU  - Beljanski, Miloš
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/542
AB  - In this paper the changes on growth, photosynthesis and water relations were tested by non-invasive fluorescence method. The applications of this method allow to determine some functional properties of prestigious maize inbred lines with erect top leaves. So the temperature dependency of delayed fluorescence intensity maximum of ZPPL 16 is observed at higher temperatures than for ZPPZ 62. This fact correlates with low values of phase transition of activation energy Ea in thylakoid membrane and accompanied by a decrease of the angle and area of the leaf, as well as with the content and the rate of water release from the seed. It seems reasonable to assume that, DF can be applied in breeding and maize hybrid seed production for the estimation of prestigious maize inbred lines and their resistance adaptability to increased and high temperatures, as well as, to drought.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence
VL  - 81
SP  - 208
EP  - 211
DO  - 10.1016/j.plaphy.2014.03.013
UR  - conv_884
ER  - 
@article{
author = "Radenović, Čedomir and Stanković, Goran and Jovanović, Života and Maksimov, Georgij and Tyutyaev, Evgenij V. and Beljanski, Miloš",
year = "2014",
abstract = "In this paper the changes on growth, photosynthesis and water relations were tested by non-invasive fluorescence method. The applications of this method allow to determine some functional properties of prestigious maize inbred lines with erect top leaves. So the temperature dependency of delayed fluorescence intensity maximum of ZPPL 16 is observed at higher temperatures than for ZPPZ 62. This fact correlates with low values of phase transition of activation energy Ea in thylakoid membrane and accompanied by a decrease of the angle and area of the leaf, as well as with the content and the rate of water release from the seed. It seems reasonable to assume that, DF can be applied in breeding and maize hybrid seed production for the estimation of prestigious maize inbred lines and their resistance adaptability to increased and high temperatures, as well as, to drought.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence",
volume = "81",
pages = "208-211",
doi = "10.1016/j.plaphy.2014.03.013",
url = "conv_884"
}
Radenović, Č., Stanković, G., Jovanović, Ž., Maksimov, G., Tyutyaev, E. V.,& Beljanski, M.. (2014). Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 81, 208-211.
https://doi.org/10.1016/j.plaphy.2014.03.013
conv_884
Radenović Č, Stanković G, Jovanović Ž, Maksimov G, Tyutyaev EV, Beljanski M. Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence. in Plant Physiology and Biochemistry. 2014;81:208-211.
doi:10.1016/j.plaphy.2014.03.013
conv_884 .
Radenović, Čedomir, Stanković, Goran, Jovanović, Života, Maksimov, Georgij, Tyutyaev, Evgenij V., Beljanski, Miloš, "Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence" in Plant Physiology and Biochemistry, 81 (2014):208-211,
https://doi.org/10.1016/j.plaphy.2014.03.013 .,
conv_884 .
4
3
5

Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions

Ignjatović-Micić, Dragana; Kostadinović, Marija; Stanković, Goran; Marković, Ksenija; Vančetović, Jelena; Božinović, Sofija; Anđelković, Violeta

(Maydica-Ist Sper Cerealicoltur, Bergamo, 2013)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Stanković, Goran
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Anđelković, Violeta
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/514
AB  - Quality protein maize (QPM) is high lysine (tryptophan) maize with hard endosperm and good agronomic performance. QPM was developed primarily for utilization in tropical and sub-tropical regions where maize is a staple food. Its adaptation and cultivation in temperate areas is still not fully developed, although QPM could have merits for production and consumption in developed parts of the world, especially as animal feed. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The objective of our research was initial screening of 72 hybrids (derived from crosses between QPM lines adapted to temperate environments and three commercial lines with standard kernel quality) for kernel modification, tryptophan and protein contents, quality index (QI) and grain yield. Five hybrids with high tryptophan content (0.071 to 0.081%) and yield at the level of standard hybrids (96 - 114%) were identified. Protein content ranged from 10 to 11.20%, similar to standard hybrids. QI was in the range from 0.71 to 0.74, which was better than in standard hybrids (0.57 - 0.62), but below the QPM threshold of 0.80. The percentage of good kernel modifications (type 1 and 2) was over 80% in three and over 74% in two hybrids. The results indicated the complexity of obtaining high yielding hybrids with high levels of essential amino-acids. Identified potential QPM hybrids have to be further evaluated for agronomic traits, but the results can be considered important in the context of limited information on QPM adapted to temperate environmental conditions.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions
VL  - 58
IS  - 1-4
SP  - 311
EP  - 317
UR  - conv_666
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Kostadinović, Marija and Stanković, Goran and Marković, Ksenija and Vančetović, Jelena and Božinović, Sofija and Anđelković, Violeta",
year = "2013",
abstract = "Quality protein maize (QPM) is high lysine (tryptophan) maize with hard endosperm and good agronomic performance. QPM was developed primarily for utilization in tropical and sub-tropical regions where maize is a staple food. Its adaptation and cultivation in temperate areas is still not fully developed, although QPM could have merits for production and consumption in developed parts of the world, especially as animal feed. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The objective of our research was initial screening of 72 hybrids (derived from crosses between QPM lines adapted to temperate environments and three commercial lines with standard kernel quality) for kernel modification, tryptophan and protein contents, quality index (QI) and grain yield. Five hybrids with high tryptophan content (0.071 to 0.081%) and yield at the level of standard hybrids (96 - 114%) were identified. Protein content ranged from 10 to 11.20%, similar to standard hybrids. QI was in the range from 0.71 to 0.74, which was better than in standard hybrids (0.57 - 0.62), but below the QPM threshold of 0.80. The percentage of good kernel modifications (type 1 and 2) was over 80% in three and over 74% in two hybrids. The results indicated the complexity of obtaining high yielding hybrids with high levels of essential amino-acids. Identified potential QPM hybrids have to be further evaluated for agronomic traits, but the results can be considered important in the context of limited information on QPM adapted to temperate environmental conditions.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions",
volume = "58",
number = "1-4",
pages = "311-317",
url = "conv_666"
}
Ignjatović-Micić, D., Kostadinović, M., Stanković, G., Marković, K., Vančetović, J., Božinović, S.,& Anđelković, V.. (2013). Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions. in Maydica
Maydica-Ist Sper Cerealicoltur, Bergamo., 58(1-4), 311-317.
conv_666
Ignjatović-Micić D, Kostadinović M, Stanković G, Marković K, Vančetović J, Božinović S, Anđelković V. Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions. in Maydica. 2013;58(1-4):311-317.
conv_666 .
Ignjatović-Micić, Dragana, Kostadinović, Marija, Stanković, Goran, Marković, Ksenija, Vančetović, Jelena, Božinović, Sofija, Anđelković, Violeta, "Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions" in Maydica, 58, no. 1-4 (2013):311-317,
conv_666 .
6
9

Protein quality analysis of F2 maize kernels

Kostadinović, Marija; Ignjatović-Micić, Dragana; Stanković, Goran; Mladenović Drinić, Snežana

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2012)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Mladenović Drinić, Snežana
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/440
AB  - Protein quality of standard maize kernels is poor, due to the low levels of two essential aminoacids - lysine and tryptophan. The opaque2 mutation which increases lysine and tryptophan content also confers an undesirable phenotype leading to low yields, soft and chalky kernels that renders seeds susceptible to storage pest and ear rots. Quality protein maize (QPM) is the maize with increased levels of these amino acids and good agronomic performances. At the Maize Research Institute, a program on creating QPM germplasm developed for conditions of temperate climatic zone is in progress. The aim of this study was to test quality traits of F2 kernels derived from the crosses between 24 QPM inbred lines with three standard inbred lines and two opaque2 lines. Most of the analyzed 108 genotypes had tryptophan content under the threshold of 0.075 set for QPM (83.5%). However, 50% had elevated tryptophan content in the range from 0.065 to 0.074. High tryptophan content was found in 16.5% of the analyzed genotypes, the highest being 0.088. Only one genotype had quality index over 0.80 (threshold for QPM). Biochemical analysis identified genotypes with good protein quality which will be further tested for agronomic performances in order to potentially be considered as QPM hybrids.
AB  - Proteini kukuruza su siromašni u sadržaju dve esencijalne aminokiseline - lizina i triptofana. Prirodna opaque2 mutacija udvostručava nivo ovih aminokiselina, ali istovremeno smanjuje prinos, čini endosperm kukuruza mekim i brašnjavim, a seme osetljivim na trulež klipa i štetočine skladištenog zrna. Kukuruz visokog kvaliteta proteina (Quality Protein Maize - QPM) se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma i istovremeno dobrim agronomskim performansama. U Institutu za kukuruz već nekoliko godina postoji program stvaranja genotipova kukuruza visokog kvaliteta proteina, adaptiranih na umereno klimatsko područje. Cilj ovog rada je bio da se ispita kvalitet proteina F2 zrna kukuruza dobijenog ukrštanjem 24 QPM inbred linija sa tri standardne inbred linije i dve opaque2 linije. Većina od 108 analiziranih genotipova (83,5%) je imala sadržaj triptofana ispod praga od 0,075, od čega je poboljšani nivo triptofana (0,065-0,074) imalo 50% genotipova. Visok sadržaj je imalo 16,5% analiziranih genotipova, od kojih je najviša vrednost bila 0,088. Samo jedan genotip je imao indeks kvaliteta proteina iznad praga od 0,80. Biohemijskom analizom utvrđeni su genotipovi poboljšanog kvaliteta čija će agronomska svojstva biti ispitana kako bi se utvrdilo da li se mogu smatrati potencijalnim QPM hibridima.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Protein quality analysis of F2 maize kernels
T1  - Biohemijska analiza F2 zrna genotipova kukuruza visokog kvaliteta proteina
VL  - 18
IS  - 1
SP  - 33
EP  - 39
UR  - conv_287
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Mladenović Drinić, Snežana",
year = "2012",
abstract = "Protein quality of standard maize kernels is poor, due to the low levels of two essential aminoacids - lysine and tryptophan. The opaque2 mutation which increases lysine and tryptophan content also confers an undesirable phenotype leading to low yields, soft and chalky kernels that renders seeds susceptible to storage pest and ear rots. Quality protein maize (QPM) is the maize with increased levels of these amino acids and good agronomic performances. At the Maize Research Institute, a program on creating QPM germplasm developed for conditions of temperate climatic zone is in progress. The aim of this study was to test quality traits of F2 kernels derived from the crosses between 24 QPM inbred lines with three standard inbred lines and two opaque2 lines. Most of the analyzed 108 genotypes had tryptophan content under the threshold of 0.075 set for QPM (83.5%). However, 50% had elevated tryptophan content in the range from 0.065 to 0.074. High tryptophan content was found in 16.5% of the analyzed genotypes, the highest being 0.088. Only one genotype had quality index over 0.80 (threshold for QPM). Biochemical analysis identified genotypes with good protein quality which will be further tested for agronomic performances in order to potentially be considered as QPM hybrids., Proteini kukuruza su siromašni u sadržaju dve esencijalne aminokiseline - lizina i triptofana. Prirodna opaque2 mutacija udvostručava nivo ovih aminokiselina, ali istovremeno smanjuje prinos, čini endosperm kukuruza mekim i brašnjavim, a seme osetljivim na trulež klipa i štetočine skladištenog zrna. Kukuruz visokog kvaliteta proteina (Quality Protein Maize - QPM) se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma i istovremeno dobrim agronomskim performansama. U Institutu za kukuruz već nekoliko godina postoji program stvaranja genotipova kukuruza visokog kvaliteta proteina, adaptiranih na umereno klimatsko područje. Cilj ovog rada je bio da se ispita kvalitet proteina F2 zrna kukuruza dobijenog ukrštanjem 24 QPM inbred linija sa tri standardne inbred linije i dve opaque2 linije. Većina od 108 analiziranih genotipova (83,5%) je imala sadržaj triptofana ispod praga od 0,075, od čega je poboljšani nivo triptofana (0,065-0,074) imalo 50% genotipova. Visok sadržaj je imalo 16,5% analiziranih genotipova, od kojih je najviša vrednost bila 0,088. Samo jedan genotip je imao indeks kvaliteta proteina iznad praga od 0,80. Biohemijskom analizom utvrđeni su genotipovi poboljšanog kvaliteta čija će agronomska svojstva biti ispitana kako bi se utvrdilo da li se mogu smatrati potencijalnim QPM hibridima.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Protein quality analysis of F2 maize kernels, Biohemijska analiza F2 zrna genotipova kukuruza visokog kvaliteta proteina",
volume = "18",
number = "1",
pages = "33-39",
url = "conv_287"
}
Kostadinović, M., Ignjatović-Micić, D., Stanković, G.,& Mladenović Drinić, S.. (2012). Protein quality analysis of F2 maize kernels. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 18(1), 33-39.
conv_287
Kostadinović M, Ignjatović-Micić D, Stanković G, Mladenović Drinić S. Protein quality analysis of F2 maize kernels. in Selekcija i semenarstvo. 2012;18(1):33-39.
conv_287 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Mladenović Drinić, Snežana, "Protein quality analysis of F2 maize kernels" in Selekcija i semenarstvo, 18, no. 1 (2012):33-39,
conv_287 .

An assessment of nutritional quality of hybrid maize grain based on chemical composition

Stevanović, Milan; Mladenović Drinić, Snežana; Dragičević, Vesna; Čamdžija, Zoran; Filipović, Milomir; Veličković, Nevena; Stanković, Goran

(Društvo genetičara Srbije, Beograd, 2012)

TY  - JOUR
AU  - Stevanović, Milan
AU  - Mladenović Drinić, Snežana
AU  - Dragičević, Vesna
AU  - Čamdžija, Zoran
AU  - Filipović, Milomir
AU  - Veličković, Nevena
AU  - Stanković, Goran
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/439
AB  - The aim of this study was to investigate chemical composition of grain of 20 maize hybrids, from different maturity groups, to define their nutritional quality from the point of main grain constituents: starch, total proteins, soluble proteins, oil, phytate, inorganic phosphorus, and soluble phenolics, as well as mass of 1.000 grains. A set of 20 ZP hybrids, FAO 400-800, were grown in a randomized complete block design (RCBD) at Zemun Polje (Serbia), during the summer of 2009. Average content of proteins, oil and starch of analyzed maize hybrids was 10.5%, 4.7% and 80.8%, respectively. Hybrids from FAO maturity group 600 had highest grain mass (in average 419.5 g). Genetic variability in seed phytate content was observed, with values ranging from 2.64 to 3.75, averaged 3.44 g kg-1. Inorganic phosphorus (Pi) concentrations were between 0.120 µ g g-1 (ZP 805) to 0.415 µ g g-1 (ZP 588ex), averaged 0.24 µ g g-1. Content of soluble proteins varied from 54.33 to 78.74 mg g-1. The high positive correlation was observed between total proteins and oil content in hybrids, as well as between starch and phenolics content. Phytate content was positively correlated with proteins, but this correlation was not significant, while the correlation between phytate and mass of 1,000 grains was positive and significant. A negative correlation occurred between phytate and starch.
AB  - U ovom radu je analizirana hemijska kompozicija zrna 20 hibrida kukuruza različitih FAO grupa zrenja sa ciljem da se definiše njihova nutritivna vrednost na osnovu osnovnih biohemijskih komponenti zrna: skroba, proteina, ulja, fitata neorganskog fosfora, fenola i mase 1000 zrna. Eksperiment je postavljen u u blok dizajnu (RCBD) na eksperimentalnim poljima Instituta za kukuruz u Zemun Polju tokom sezone 2009. Prosečne vrednosti proteina, ulja i skroba analiziranih hibrida kukuruza iznosile su 10.5%, 4.7% and 80.8%, respektivno. Hibridi FAO grupe zrenja 600 imali su najveću masu 1000 zrna (u proseku 419.5 g). Uočena je varijabilnost u sadržaju fitina kod analiziranih hibrida i ona je iznosila od 2.64 do 3.75, sa prosečnom vrednošću od 3.44 g kg-1. Koncentracija neorganskog fosfor (Pi) se kretala od 0.120 µg g-1 (ZP 805) do 0.415 µ g g-1 (ZP 588ex), sa prosekom od 0.24 µ g g-1. Sadržaj solubilnih proteina je varirao od 54.33 do 78.74 mg g-1. Visoko pozitivna korelacija uočena je između sadržaja protein i ulja u zrnu, kao i između skroba i fenola. Fitat je bio u pozitivnoj korelaciji sa proteinima, ali ova korelacija nije bila statistički značajna, dok je korelacija između fitata i mase 1000 zrna pozitivna i signifikantna. Negativna korelacija je uočena između fitata i skroba.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - An assessment of nutritional quality of hybrid maize grain based on chemical composition
T1  - Procena nutritivnog kvaliteta hibrida kukuruza na osnovu hemijske kompozicije zrna
VL  - 44
IS  - 3
SP  - 571
EP  - 582
DO  - 10.2298/GENSR1203571S
UR  - conv_467
ER  - 
@article{
author = "Stevanović, Milan and Mladenović Drinić, Snežana and Dragičević, Vesna and Čamdžija, Zoran and Filipović, Milomir and Veličković, Nevena and Stanković, Goran",
year = "2012",
abstract = "The aim of this study was to investigate chemical composition of grain of 20 maize hybrids, from different maturity groups, to define their nutritional quality from the point of main grain constituents: starch, total proteins, soluble proteins, oil, phytate, inorganic phosphorus, and soluble phenolics, as well as mass of 1.000 grains. A set of 20 ZP hybrids, FAO 400-800, were grown in a randomized complete block design (RCBD) at Zemun Polje (Serbia), during the summer of 2009. Average content of proteins, oil and starch of analyzed maize hybrids was 10.5%, 4.7% and 80.8%, respectively. Hybrids from FAO maturity group 600 had highest grain mass (in average 419.5 g). Genetic variability in seed phytate content was observed, with values ranging from 2.64 to 3.75, averaged 3.44 g kg-1. Inorganic phosphorus (Pi) concentrations were between 0.120 µ g g-1 (ZP 805) to 0.415 µ g g-1 (ZP 588ex), averaged 0.24 µ g g-1. Content of soluble proteins varied from 54.33 to 78.74 mg g-1. The high positive correlation was observed between total proteins and oil content in hybrids, as well as between starch and phenolics content. Phytate content was positively correlated with proteins, but this correlation was not significant, while the correlation between phytate and mass of 1,000 grains was positive and significant. A negative correlation occurred between phytate and starch., U ovom radu je analizirana hemijska kompozicija zrna 20 hibrida kukuruza različitih FAO grupa zrenja sa ciljem da se definiše njihova nutritivna vrednost na osnovu osnovnih biohemijskih komponenti zrna: skroba, proteina, ulja, fitata neorganskog fosfora, fenola i mase 1000 zrna. Eksperiment je postavljen u u blok dizajnu (RCBD) na eksperimentalnim poljima Instituta za kukuruz u Zemun Polju tokom sezone 2009. Prosečne vrednosti proteina, ulja i skroba analiziranih hibrida kukuruza iznosile su 10.5%, 4.7% and 80.8%, respektivno. Hibridi FAO grupe zrenja 600 imali su najveću masu 1000 zrna (u proseku 419.5 g). Uočena je varijabilnost u sadržaju fitina kod analiziranih hibrida i ona je iznosila od 2.64 do 3.75, sa prosečnom vrednošću od 3.44 g kg-1. Koncentracija neorganskog fosfor (Pi) se kretala od 0.120 µg g-1 (ZP 805) do 0.415 µ g g-1 (ZP 588ex), sa prosekom od 0.24 µ g g-1. Sadržaj solubilnih proteina je varirao od 54.33 do 78.74 mg g-1. Visoko pozitivna korelacija uočena je između sadržaja protein i ulja u zrnu, kao i između skroba i fenola. Fitat je bio u pozitivnoj korelaciji sa proteinima, ali ova korelacija nije bila statistički značajna, dok je korelacija između fitata i mase 1000 zrna pozitivna i signifikantna. Negativna korelacija je uočena između fitata i skroba.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "An assessment of nutritional quality of hybrid maize grain based on chemical composition, Procena nutritivnog kvaliteta hibrida kukuruza na osnovu hemijske kompozicije zrna",
volume = "44",
number = "3",
pages = "571-582",
doi = "10.2298/GENSR1203571S",
url = "conv_467"
}
Stevanović, M., Mladenović Drinić, S., Dragičević, V., Čamdžija, Z., Filipović, M., Veličković, N.,& Stanković, G.. (2012). An assessment of nutritional quality of hybrid maize grain based on chemical composition. in Genetika
Društvo genetičara Srbije, Beograd., 44(3), 571-582.
https://doi.org/10.2298/GENSR1203571S
conv_467
Stevanović M, Mladenović Drinić S, Dragičević V, Čamdžija Z, Filipović M, Veličković N, Stanković G. An assessment of nutritional quality of hybrid maize grain based on chemical composition. in Genetika. 2012;44(3):571-582.
doi:10.2298/GENSR1203571S
conv_467 .
Stevanović, Milan, Mladenović Drinić, Snežana, Dragičević, Vesna, Čamdžija, Zoran, Filipović, Milomir, Veličković, Nevena, Stanković, Goran, "An assessment of nutritional quality of hybrid maize grain based on chemical composition" in Genetika, 44, no. 3 (2012):571-582,
https://doi.org/10.2298/GENSR1203571S .,
conv_467 .
1
1
3

Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties

Lević, Jelena; Petrović, Tijana; Stanković, Slavica; Stanković, Goran; Krnjaja, Vesna

(Natl Agricultural Research & Development Inst, Fundulea, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Petrović, Tijana
AU  - Stanković, Slavica
AU  - Stanković, Goran
AU  - Krnjaja, Vesna
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/412
AB  - Rot symptoms that Pyrenochaeta terrestris causes on the root of each internode of maize hybrids belonging to different maturity groups, as well as the impact of growing conditions (substrate, temperature and light) on the properties of the colony and the pycnidial formation of this fungus were studied. The large number of symptoms was obtained by various combinations of tissue colour changes (red, brown, yellow, blue and lighter or shades of these colours), changes in a particular part of the root (root top, part of the epidermis, the entire epidermis, tissue under the epidermis or the whole root) and the form of spots and streaks (shape and size). Pinkish red symptoms prevailed on younger roots, particularly from the 5th to the 7th internode. When P. terrestris was grown on potato dextrose agar (PDA) in the dark at 25 degrees C, the variability of the fungus was expressed in relation to the colour of the aerial (white, pink, grey, yellow and brown) and the substrate mycelium (purple, pink, grey, green and yellowish) and to the pycnidial formation (present or absent). The interrelationship between types of symptoms and properties of P. terrestris colonies was not determined on PDA. The fungus always produced the red purple pigment and mostly pycnidia on carnation leaf agar (CLA) at 25 degrees C and under the alternating 12 h combined light (fluorescent and near ultra violet (NUV) light)/dark conditions. These conditions are suitable for the reliable identification of the fungus.
PB  - Natl Agricultural Research & Development Inst, Fundulea
T2  - Romanian Agricultural Research
T1  - Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties
VL  - 29
SP  - 339
EP  - 347
UR  - conv_830
ER  - 
@article{
author = "Lević, Jelena and Petrović, Tijana and Stanković, Slavica and Stanković, Goran and Krnjaja, Vesna",
year = "2012",
abstract = "Rot symptoms that Pyrenochaeta terrestris causes on the root of each internode of maize hybrids belonging to different maturity groups, as well as the impact of growing conditions (substrate, temperature and light) on the properties of the colony and the pycnidial formation of this fungus were studied. The large number of symptoms was obtained by various combinations of tissue colour changes (red, brown, yellow, blue and lighter or shades of these colours), changes in a particular part of the root (root top, part of the epidermis, the entire epidermis, tissue under the epidermis or the whole root) and the form of spots and streaks (shape and size). Pinkish red symptoms prevailed on younger roots, particularly from the 5th to the 7th internode. When P. terrestris was grown on potato dextrose agar (PDA) in the dark at 25 degrees C, the variability of the fungus was expressed in relation to the colour of the aerial (white, pink, grey, yellow and brown) and the substrate mycelium (purple, pink, grey, green and yellowish) and to the pycnidial formation (present or absent). The interrelationship between types of symptoms and properties of P. terrestris colonies was not determined on PDA. The fungus always produced the red purple pigment and mostly pycnidia on carnation leaf agar (CLA) at 25 degrees C and under the alternating 12 h combined light (fluorescent and near ultra violet (NUV) light)/dark conditions. These conditions are suitable for the reliable identification of the fungus.",
publisher = "Natl Agricultural Research & Development Inst, Fundulea",
journal = "Romanian Agricultural Research",
title = "Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties",
volume = "29",
pages = "339-347",
url = "conv_830"
}
Lević, J., Petrović, T., Stanković, S., Stanković, G.,& Krnjaja, V.. (2012). Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties. in Romanian Agricultural Research
Natl Agricultural Research & Development Inst, Fundulea., 29, 339-347.
conv_830
Lević J, Petrović T, Stanković S, Stanković G, Krnjaja V. Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties. in Romanian Agricultural Research. 2012;29:339-347.
conv_830 .
Lević, Jelena, Petrović, Tijana, Stanković, Slavica, Stanković, Goran, Krnjaja, Vesna, "Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties" in Romanian Agricultural Research, 29 (2012):339-347,
conv_830 .
3
3

Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain

Stanković, Slavica; Lević, Jelena; Ivanović, Dragica; Stanković, Goran; Krnjaja, Vesna; Tančić, Sonja

(Elsevier Sci Ltd, Oxford, 2012)

TY  - JOUR
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Ivanović, Dragica
AU  - Stanković, Goran
AU  - Krnjaja, Vesna
AU  - Tančić, Sonja
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/420
AB  - The natural occurrence of fumonisin B-1 (FB1) and its co-occurrence with zearalenone (ZEA), T-2 toxin and deoxynivalenol (DON) were surveyed in 103 winter wheat samples collected after four to six-month storage in family barns from different locations in Serbia. All 103 samples were mycotoxin positive. The mean concentrations of all mycotoxins except ZEA were greater in 2005 than in 2007. FB1 was detected in 82.1% and 92.0% of all samples with ranges of 750-5400 mu g kg(-1) (mean, 2079.45 mu g kg(-1)) and 750-4900 mu g kg(-1) (mean 918.76 mu g kg(-1)) in 2005 and 2007, respectively. Moderate positive correlations were found between FB1 and DON concentrations (r = 0.56 in 2005 and r = 0.54 in 2007) and between FB1 and ZEA concentrations (r = 0.48 in 2005 and r = 0.60 in 2007), while a moderate negative correlation was detected between the production of FB1 and T-2 toxin in 2007 (r = -0.33). This is the first report of FB1 occurrence in naturally-contaminated wheat grain and its simultaneous occurrence with ZEA, DON and T-2 toxin in Serbia. Moreover, this is one of the rare reports presenting the occurrence of FB1 on wheat in the world.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain
VL  - 23
IS  - 2
SP  - 384
EP  - 388
DO  - 10.1016/j.foodcont.2011.08.003
UR  - conv_794
ER  - 
@article{
author = "Stanković, Slavica and Lević, Jelena and Ivanović, Dragica and Stanković, Goran and Krnjaja, Vesna and Tančić, Sonja",
year = "2012",
abstract = "The natural occurrence of fumonisin B-1 (FB1) and its co-occurrence with zearalenone (ZEA), T-2 toxin and deoxynivalenol (DON) were surveyed in 103 winter wheat samples collected after four to six-month storage in family barns from different locations in Serbia. All 103 samples were mycotoxin positive. The mean concentrations of all mycotoxins except ZEA were greater in 2005 than in 2007. FB1 was detected in 82.1% and 92.0% of all samples with ranges of 750-5400 mu g kg(-1) (mean, 2079.45 mu g kg(-1)) and 750-4900 mu g kg(-1) (mean 918.76 mu g kg(-1)) in 2005 and 2007, respectively. Moderate positive correlations were found between FB1 and DON concentrations (r = 0.56 in 2005 and r = 0.54 in 2007) and between FB1 and ZEA concentrations (r = 0.48 in 2005 and r = 0.60 in 2007), while a moderate negative correlation was detected between the production of FB1 and T-2 toxin in 2007 (r = -0.33). This is the first report of FB1 occurrence in naturally-contaminated wheat grain and its simultaneous occurrence with ZEA, DON and T-2 toxin in Serbia. Moreover, this is one of the rare reports presenting the occurrence of FB1 on wheat in the world.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain",
volume = "23",
number = "2",
pages = "384-388",
doi = "10.1016/j.foodcont.2011.08.003",
url = "conv_794"
}
Stanković, S., Lević, J., Ivanović, D., Stanković, G., Krnjaja, V.,& Tančić, S.. (2012). Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain. in Food Control
Elsevier Sci Ltd, Oxford., 23(2), 384-388.
https://doi.org/10.1016/j.foodcont.2011.08.003
conv_794
Stanković S, Lević J, Ivanović D, Stanković G, Krnjaja V, Tančić S. Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain. in Food Control. 2012;23(2):384-388.
doi:10.1016/j.foodcont.2011.08.003
conv_794 .
Stanković, Slavica, Lević, Jelena, Ivanović, Dragica, Stanković, Goran, Krnjaja, Vesna, Tančić, Sonja, "Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain" in Food Control, 23, no. 2 (2012):384-388,
https://doi.org/10.1016/j.foodcont.2011.08.003 .,
conv_794 .
53
43
49