Vasić, Marko G.

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  • Vasić, Marko G. (3)
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Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Perić, Vesna; Srdić, Jelena; Vasić, Marko G.

(Novi Sad : National Society of Processing and Energy in Agriculture, 2022-03-17)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Perić, Vesna
AU  - Srdić, Jelena
AU  - Vasić, Marko G.
PY  - 2022-03-17
UR  - http://rik.mrizp.rs/handle/123456789/847
AB  - Black  soybean  seed  coat  has  a  considerable  content  of  anthocyanins  which  can  providea  positive  effect  on  the  health  of  the  consumers through food products enriched with the extracts of these valuable bioactive compounds. Nevertheless, the use of soybean seed  coat,  a  by-product  from  soybean  processing,  additionally  valorizes  this  raw  material.The  possibility  of  enrichment  of  sweet maize  grains  with  anthocyanin  extract  was  investigated.  Several  procedures  with  acetic  acid  were  applied  in  the  experiments  of  anthocyanins  extraction  from  black  soybean  seed  coat.  During  the  marination  process,  the  grains  were  stained,  changing  their  standard yellow color to a crimson red. Since the brines with the addition of citric acid became cloudy (opalescent) after some time, lactic  acid  was  used  as  an  anthocyanin  stabilizer  in  the  continuation  of  the  research.  Depending  on  the  extraction  conditions,  the spectrophotometrically determined content of total anthocyanins in the solution varied from 3541.90 to 5387.70 μg CGE/g d.m., and in the marinated maize grain between 179.89 and 286.05 μg CGE/g d.m. After seven days, the total anthocyanin content in the grain did not increase significantly, so this aging period was selected as optimal for marinating maize products.
AB  - Semenjača  crne  soje  ima  značajan  sadržaj  antocijana,  koji  mogu  pozitivno  uticati  na  zdravlje  potrošača  kroz  prehrambene  proizvode  obogaćene  ekstraktima  ovih  vrednih  bioaktivnih  jedinjenja.  Istovremeno,  upotrebom  sojine  semenjače,  sporednog  proizvoda prerade soje, dodatno se valorizuje ova sirovinu. Ispitivana je mogućnost obogaćivanja zrna kukuruza šećerca ekstraktom antocijana.  U  eksperimentima  ekstrakcije  antocijana  iz  semenjače  crne  soje  primenjeno  je  nekoliko  postupaka  sa  sirćetnom kiselinom.  Tokom  procesa  mariniranja,  zrna  su  promenila  boju,  menjajući  standardnu  žutu  boju  u  tamnocrvenu.  S  obzirom  da  su  posle izvesnog vremena nalivi za mariniranje sa dodatkom limunske kiseline postali zamućeni (opalescentni), u nastavku istraživanja je  korišćena  mlečna  kiselina  kao  stabilizator  antocijana.  U  zavisnosti  od  uslova  ekstrakcije,  spektrofotometrijski  utvrđen  sadržaj  ukupnih  antocijana  u  rastvoru  varirao  je  od  3541,90  do  5387,70  mg  CGE/g  d.m.,  a  u  zrnu  mariniranog  kukuruza  između  179,89  i  286,05  mg  CGE/g.  Posle  sedam  dana,  ukupan  sadržaj  antocijana  u  zrnu  nije  značajno  povećan,  pa  je  ovaj  period  odležavanja  izabran kao optimalan za mariniranje proizvoda od kukuruza.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food
T1  - Optimizacija procesa ekstrakcije antocijana iz semenjače crne soje za pripremu funkcionalne hrane od kukuruza
VL  - 26
IS  - 1
SP  - 19
EP  - 22
DO  - 10.5937/jpea%v-36635
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Perić, Vesna and Srdić, Jelena and Vasić, Marko G.",
year = "2022-03-17",
abstract = "Black  soybean  seed  coat  has  a  considerable  content  of  anthocyanins  which  can  providea  positive  effect  on  the  health  of  the  consumers through food products enriched with the extracts of these valuable bioactive compounds. Nevertheless, the use of soybean seed  coat,  a  by-product  from  soybean  processing,  additionally  valorizes  this  raw  material.The  possibility  of  enrichment  of  sweet maize  grains  with  anthocyanin  extract  was  investigated.  Several  procedures  with  acetic  acid  were  applied  in  the  experiments  of  anthocyanins  extraction  from  black  soybean  seed  coat.  During  the  marination  process,  the  grains  were  stained,  changing  their  standard yellow color to a crimson red. Since the brines with the addition of citric acid became cloudy (opalescent) after some time, lactic  acid  was  used  as  an  anthocyanin  stabilizer  in  the  continuation  of  the  research.  Depending  on  the  extraction  conditions,  the spectrophotometrically determined content of total anthocyanins in the solution varied from 3541.90 to 5387.70 μg CGE/g d.m., and in the marinated maize grain between 179.89 and 286.05 μg CGE/g d.m. After seven days, the total anthocyanin content in the grain did not increase significantly, so this aging period was selected as optimal for marinating maize products., Semenjača  crne  soje  ima  značajan  sadržaj  antocijana,  koji  mogu  pozitivno  uticati  na  zdravlje  potrošača  kroz  prehrambene  proizvode  obogaćene  ekstraktima  ovih  vrednih  bioaktivnih  jedinjenja.  Istovremeno,  upotrebom  sojine  semenjače,  sporednog  proizvoda prerade soje, dodatno se valorizuje ova sirovinu. Ispitivana je mogućnost obogaćivanja zrna kukuruza šećerca ekstraktom antocijana.  U  eksperimentima  ekstrakcije  antocijana  iz  semenjače  crne  soje  primenjeno  je  nekoliko  postupaka  sa  sirćetnom kiselinom.  Tokom  procesa  mariniranja,  zrna  su  promenila  boju,  menjajući  standardnu  žutu  boju  u  tamnocrvenu.  S  obzirom  da  su  posle izvesnog vremena nalivi za mariniranje sa dodatkom limunske kiseline postali zamućeni (opalescentni), u nastavku istraživanja je  korišćena  mlečna  kiselina  kao  stabilizator  antocijana.  U  zavisnosti  od  uslova  ekstrakcije,  spektrofotometrijski  utvrđen  sadržaj  ukupnih  antocijana  u  rastvoru  varirao  je  od  3541,90  do  5387,70  mg  CGE/g  d.m.,  a  u  zrnu  mariniranog  kukuruza  između  179,89  i  286,05  mg  CGE/g.  Posle  sedam  dana,  ukupan  sadržaj  antocijana  u  zrnu  nije  značajno  povećan,  pa  je  ovaj  period  odležavanja  izabran kao optimalan za mariniranje proizvoda od kukuruza.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food, Optimizacija procesa ekstrakcije antocijana iz semenjače crne soje za pripremu funkcionalne hrane od kukuruza",
volume = "26",
number = "1",
pages = "19-22",
doi = "10.5937/jpea%v-36635"
}
Nikolić, V., Žilić, S., Simić, M., Perić, V., Srdić, J.,& Vasić, M. G.. (2022-03-17). Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 26(1), 19-22.
https://doi.org/10.5937/jpea%v-36635
Nikolić V, Žilić S, Simić M, Perić V, Srdić J, Vasić MG. Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food. in Journal on Processing and Energy in Agriculture. 2022;26(1):19-22.
doi:10.5937/jpea%v-36635 .
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Perić, Vesna, Srdić, Jelena, Vasić, Marko G., "Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food" in Journal on Processing and Energy in Agriculture, 26, no. 1 (2022-03-17):19-22,
https://doi.org/10.5937/jpea%v-36635 . .

Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Vasić, Marko G.; Srdić, Jelena; Delić, Nenad; Delić, Nenad

(Novi Sad : National Society of Processing and Energy in Agriculture, 2021-05-07)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Vasić, Marko G.
AU  - Srdić, Jelena
AU  - Delić, Nenad
AU  - Delić, Nenad
PY  - 2021-05-07
UR  - http://rik.mrizp.rs/handle/123456789/846
AB  - Five maize hybrids were used in this research: two yellow and one white dent, sweet hybrid, and yellow popcorn. Five brine
recipes with acetic acid were examined, of which two with the addition of potassium sorbate. The brines with preservatives were
stable for 16 months without colour changes of the liquid and corn cobs. The pH of brines ranged from 3.39 to 3.89. Canned hybrids
ZP 366 and ZP 611k in brine without sugar, and with the addition of potassium sorbate and potassium metabisulfite marked as
Number 5, showed the best sensory characteristics. The protein content determined in ZP 366 (9.56 %) and ZP 611k (10.23 %) did
not vary significantly compared to whole-grain maize flour, while crude fibre content (7.67 and 6.88 %), and ash content (21.96 and
20.72 %) were significantly higher than in flour (crude fibre: 2.40; 2.64 %, ash:1.35; 1.48 %, respectively). This research will be
continued in order to implement preliminary findings and new data on this subject
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane
T1  - Possibilities of maize hybrids utilization in canned baby corn production
VL  - 25
IS  - 1
SP  - 16
EP  - 19
DO  - 10.5937/jpea25-30887
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Vasić, Marko G. and Srdić, Jelena and Delić, Nenad and Delić, Nenad",
year = "2021-05-07",
abstract = "Five maize hybrids were used in this research: two yellow and one white dent, sweet hybrid, and yellow popcorn. Five brine
recipes with acetic acid were examined, of which two with the addition of potassium sorbate. The brines with preservatives were
stable for 16 months without colour changes of the liquid and corn cobs. The pH of brines ranged from 3.39 to 3.89. Canned hybrids
ZP 366 and ZP 611k in brine without sugar, and with the addition of potassium sorbate and potassium metabisulfite marked as
Number 5, showed the best sensory characteristics. The protein content determined in ZP 366 (9.56 %) and ZP 611k (10.23 %) did
not vary significantly compared to whole-grain maize flour, while crude fibre content (7.67 and 6.88 %), and ash content (21.96 and
20.72 %) were significantly higher than in flour (crude fibre: 2.40; 2.64 %, ash:1.35; 1.48 %, respectively). This research will be
continued in order to implement preliminary findings and new data on this subject",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane, Possibilities of maize hybrids utilization in canned baby corn production",
volume = "25",
number = "1",
pages = "16-19",
doi = "10.5937/jpea25-30887"
}
Nikolić, V., Žilić, S., Simić, M., Vasić, M. G., Srdić, J., Delić, N.,& Delić, N.. (2021-05-07). Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 25(1), 16-19.
https://doi.org/10.5937/jpea25-30887
Nikolić V, Žilić S, Simić M, Vasić MG, Srdić J, Delić N, Delić N. Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane. in Journal on Processing and Energy in Agriculture. 2021;25(1):16-19.
doi:10.5937/jpea25-30887 .
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Vasić, Marko G., Srdić, Jelena, Delić, Nenad, Delić, Nenad, "Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane" in Journal on Processing and Energy in Agriculture, 25, no. 1 (2021-05-07):16-19,
https://doi.org/10.5937/jpea25-30887 . .
1

Effects of heat processing on soya bean fatty acids content and the lipoxygenase activity

Žilić, Slađana; Šobajić, Slađana; Mladenović-Drinić, Snežana; Kresović, Branka; Vasić, Marko G.

(Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd, 2010)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Šobajić, Slađana
AU  - Mladenović-Drinić, Snežana
AU  - Kresović, Branka
AU  - Vasić, Marko G.
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/354
AB  - Effects of increased temperatures on the lipoxygenase activity and changes of soya bean fatty acids were observed in the present study. The kernels of soya bean cultivars Bosa and ZPS 015 were subjected to the treatments of extrusion, autoclaving, micronisation and microwave roasting. Depending on the technological processing procedure, the kernels were exposed to temperatures ranging from 60 to 150°C for 25 to 30 seconds during extrusion and for 30 minutes during autoclaving. The temperature that developed in the course of the microwave radiation and autoclaving did not cause statistically significant differences between oil content in heat treated and fresh kernels of soya bean. However, the oil content was higher in soya bean flakes (micronized kernels) and lower in grits than in fresh kernels. The heat treatments resulted in the significant decrease of the linolenic fatty acid content. Depending on the temperature and applied heat treatments, the content of linoleic and oleic fatty acid oscillated. High temperatures caused changes in unsaturated fatty acids with 18 carbon atoms resulting in relative increase of the stearic acid content. The lipoxygenase activity decreased in correlation with increased temperatures and the time of heating. The maximum drop of the activity was observed after kernel exposure to the extrusion and micronisation processes at the temperature of 100oC. However, a significant lipoxygenase activity increase was recorded in both studied cultivars after one-minute microwave heating, i.e. at the temperature about 60°C. A further temperature increase led to a gradual denaturation of the enzyme and therefore to its decreased activity.
AB  - U ovom radu prikazani su rezultati promene aktivnosti lipoksigenaze 1 i sadržaja masnih kiselina u sojinom zrnu pod uticajem povišene temperature. Zrno soje sorte Bosa i ZPS 015 bilo je podvrgnuto tretmanima suve i vlažne ekstruzije, autoklaviranju, mikronizaciji i prženju u mikrotalasnoj peći. U zavisnosti od tehnološkog tretmana prerade zrno je bilo izloženo dejstvu povišene temperature od 60 do 150°C u trajanju od svega 25 do 30 sekundi pri ekstruziji do 30 minuta pri autoklaviranju. Povišena temperatura tokom mikrotalasnog prženja i autoklaviranja nije uticala na sadržaj ulja u sojinom zrnu. Međutim, u sojinim flekicama sadržaj ulja bio je viši, a u grizu niži od sadržaja u sirovom sojinom zrnu. Primenjeni termički tretmani prerade uticali su na značajno smanjenje sadržaja linolenske kiseline u ulju sojinog zrna. U zavisnosti od temperature, kao i vrste termičkog tretmana sadržaj linolne i oleinske kiseline u prerađenom sojinom zrnu je varirao. Povišena temperatura uslovila je promene nezasićenih masnih kiselina sa 18 ugljenikovih atoma rezultirajući povećanje sadržaja stearinske kiseline. Prema našim rezultatima aktivnost lipoksiganaze je opadala sa povećanjem temperature i produženjem tretmana prerade. Maksimalan pad aktivnosti lipoksigenaze utvrđen je već nakon izlaganja zrna temperaturi od 100oC tokom ekstruzije i mikronizacije. Međutim, nakom jednog minuta prženja zrna u mikrotalasnoj peći (60°C) aktivnost lipoksigenaze je bila viša u odnosu na aktivnost u sirovom zrnu. Daljim produženjem tretmana, a time i povećanjem temperature došlo je do postepene denaturacije enzima i smanjenja aktivnosti.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences
T1  - Effects of heat processing on soya bean fatty acids content and the lipoxygenase activity
T1  - Uticaj termičkih tretmana prerade na sadržaj masnih kiselina sojinog zrna i aktivnost lipoksigenaze
VL  - 55
IS  - 1
SP  - 55
EP  - 64
DO  - 10.2298/JAS1001055Z
UR  - conv_561
ER  - 
@article{
author = "Žilić, Slađana and Šobajić, Slađana and Mladenović-Drinić, Snežana and Kresović, Branka and Vasić, Marko G.",
year = "2010",
abstract = "Effects of increased temperatures on the lipoxygenase activity and changes of soya bean fatty acids were observed in the present study. The kernels of soya bean cultivars Bosa and ZPS 015 were subjected to the treatments of extrusion, autoclaving, micronisation and microwave roasting. Depending on the technological processing procedure, the kernels were exposed to temperatures ranging from 60 to 150°C for 25 to 30 seconds during extrusion and for 30 minutes during autoclaving. The temperature that developed in the course of the microwave radiation and autoclaving did not cause statistically significant differences between oil content in heat treated and fresh kernels of soya bean. However, the oil content was higher in soya bean flakes (micronized kernels) and lower in grits than in fresh kernels. The heat treatments resulted in the significant decrease of the linolenic fatty acid content. Depending on the temperature and applied heat treatments, the content of linoleic and oleic fatty acid oscillated. High temperatures caused changes in unsaturated fatty acids with 18 carbon atoms resulting in relative increase of the stearic acid content. The lipoxygenase activity decreased in correlation with increased temperatures and the time of heating. The maximum drop of the activity was observed after kernel exposure to the extrusion and micronisation processes at the temperature of 100oC. However, a significant lipoxygenase activity increase was recorded in both studied cultivars after one-minute microwave heating, i.e. at the temperature about 60°C. A further temperature increase led to a gradual denaturation of the enzyme and therefore to its decreased activity., U ovom radu prikazani su rezultati promene aktivnosti lipoksigenaze 1 i sadržaja masnih kiselina u sojinom zrnu pod uticajem povišene temperature. Zrno soje sorte Bosa i ZPS 015 bilo je podvrgnuto tretmanima suve i vlažne ekstruzije, autoklaviranju, mikronizaciji i prženju u mikrotalasnoj peći. U zavisnosti od tehnološkog tretmana prerade zrno je bilo izloženo dejstvu povišene temperature od 60 do 150°C u trajanju od svega 25 do 30 sekundi pri ekstruziji do 30 minuta pri autoklaviranju. Povišena temperatura tokom mikrotalasnog prženja i autoklaviranja nije uticala na sadržaj ulja u sojinom zrnu. Međutim, u sojinim flekicama sadržaj ulja bio je viši, a u grizu niži od sadržaja u sirovom sojinom zrnu. Primenjeni termički tretmani prerade uticali su na značajno smanjenje sadržaja linolenske kiseline u ulju sojinog zrna. U zavisnosti od temperature, kao i vrste termičkog tretmana sadržaj linolne i oleinske kiseline u prerađenom sojinom zrnu je varirao. Povišena temperatura uslovila je promene nezasićenih masnih kiselina sa 18 ugljenikovih atoma rezultirajući povećanje sadržaja stearinske kiseline. Prema našim rezultatima aktivnost lipoksiganaze je opadala sa povećanjem temperature i produženjem tretmana prerade. Maksimalan pad aktivnosti lipoksigenaze utvrđen je već nakon izlaganja zrna temperaturi od 100oC tokom ekstruzije i mikronizacije. Međutim, nakom jednog minuta prženja zrna u mikrotalasnoj peći (60°C) aktivnost lipoksigenaze je bila viša u odnosu na aktivnost u sirovom zrnu. Daljim produženjem tretmana, a time i povećanjem temperature došlo je do postepene denaturacije enzima i smanjenja aktivnosti.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences",
title = "Effects of heat processing on soya bean fatty acids content and the lipoxygenase activity, Uticaj termičkih tretmana prerade na sadržaj masnih kiselina sojinog zrna i aktivnost lipoksigenaze",
volume = "55",
number = "1",
pages = "55-64",
doi = "10.2298/JAS1001055Z",
url = "conv_561"
}
Žilić, S., Šobajić, S., Mladenović-Drinić, S., Kresović, B.,& Vasić, M. G.. (2010). Effects of heat processing on soya bean fatty acids content and the lipoxygenase activity. in Journal of Agricultural Sciences
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 55(1), 55-64.
https://doi.org/10.2298/JAS1001055Z
conv_561
Žilić S, Šobajić S, Mladenović-Drinić S, Kresović B, Vasić MG. Effects of heat processing on soya bean fatty acids content and the lipoxygenase activity. in Journal of Agricultural Sciences. 2010;55(1):55-64.
doi:10.2298/JAS1001055Z
conv_561 .
Žilić, Slađana, Šobajić, Slađana, Mladenović-Drinić, Snežana, Kresović, Branka, Vasić, Marko G., "Effects of heat processing on soya bean fatty acids content and the lipoxygenase activity" in Journal of Agricultural Sciences, 55, no. 1 (2010):55-64,
https://doi.org/10.2298/JAS1001055Z .,
conv_561 .
16