TY  - JOUR
AU  - Đan, Mihajla
AU  - Veličković, Nevena
AU  - Obreht, Dragana
AU  - Kočiš-Tubić, Nataša
AU  - Marković, Vladimir
AU  - Stevanović, Milan
AU  - Beuković, Miloš
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/517
AB  - The wild boar (Sus scrofa) is one of most abundant game species in hunting areas of Balkan region. The large fraction of pre-glacial genetic diversity in wild boar populations from the Balkans was addressed due to high proportion of unique mtDNA haplotypes found in Greece, indicating Balkan as main refugial area for wild boars. The aim of the present study is to characterize mitochondrial DNA control region variability in wild boars from different areas in the West Balkan region, in order to evaluate level of genetic variability, to detect unique haplotypes and to infer possible structuring. The total number of 163 individuals from different sampling localities were included in the study. A fragment of the mtDNA control region was amplified and sequenced by standard procedures. Population genetic analyses were performed using several computer packages: BioEdit, ARLEQUIN 3.5.1.2., Network 4.6.0.0 and MEGA5. Eleven different haplotypes were identified and haplotype diversity was 0.676, nucleotide diversity 0.0026, and the average number of nucleotide differences (k) 1.169. The mismatch distribution and neutrality tests indicated the expansion of the all populations. It is shown that high level of genetic diversity is present in the wild boars from the West Balkan region and we have managed to detect regional unique haplotypes in high frequency. Genetic diversity differences have been found in regional wild boar groups, clustering them in two main clusters, but further speculations on the reasons for the observed clustering are prevented due to restricted informativness of the single locus marker. Obtained knowledge of genetic variation in the wild boar may be relevant for improving knowledge of the phylogeny and phylogeography of the wild boars, but as well as for hunting societies and responsible authorities for the effective control of wild boar populations.
AB  - Divlja svinja (Sus scrofa) je jedna od najbrojnijih vrsta divljači u lovnim područjima Balkana. Na osnovu velikog broja jedinstvenih haplotipova u populacijama divljih svinja Grčke, zaključeno je da se veliki deo pre-glacijalnog genetičkog diverziteta ovih populacija zadržao na Balkanskom poluostrvu, koje je označeno kao glavni refugijum za divlje svinje. Cilj ovog rada je karakterizacija varijabilnosti kontrolnog regiona mitohondrijalne DNK kod divljih svinja sa različitih lokaliteta Zapadnog Balkana, u svrhu utvrđivanja genetičke varijabilnosti, detekcije jedinstvenih haplotipova i definisanja moguće struktuiranosti populacije. Ukupno su analizirane 163 jedinke sa različitih lokaliteta. Deo kontrolnog regiona mtDNK je umnožen i sekvenciran standardnim metodama. Populaciono-genetička analiza urađena je pomoću programskih paketa za analizu sekvenci: BioEdit, ARLEQUIN 3.5.1.2., Network 4.6.0.0 and MEGA5. Nađeno je jedanaest haplotipova i diverzitet haplotipova je iznosio 0.676, diverzitet nukleotida 0.0026, dok je prosečan broj nukleotidnih razlika bio 1.169. Test unimodalne distribucije i testovi neutralnosti pokazali su ekspanziju svih populacija. Pokazan je visok nivo genetičkog diverziteta u populacijama divljih svinja Zapadnog Balkana i detektovani su haplotipovi jedinstveni za regione u visokoj frekvenciji. Razlike u genetičkom diverzitetu pokazane su u regionalnim grupama, i klaster analiza je pokazala prisustvo dve odvojene grane, ali konačan zaključak o stepenu struktuiranosti nije mogao biti donet, zbog ograničene informativnosti jednog genskog markera. Dobijeni rezultati o genetičkoj varijabilnosti divljih svinja relevantni su za rekonstrukciju filogenije i filogeografije ove vrste divljači, ali i za organizacije odgovorne za efektivnu kontrolu populacija divljih svinja.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Mitochondrial DNA control region variability in wild boars from west Balkans
T1  - Varijabilnost kontrolnog regiona mitohondrijalne DNK divljih svinja zapadnog Balkana
VL  - 45
IS  - 2
SP  - 515
EP  - 526
DO  - 10.2298/GENSR1302515D
ER  - 

@article{
author = "Đan, Mihajla and Veličković, Nevena and Obreht, Dragana and Kočiš-Tubić, Nataša and Marković, Vladimir and Stevanović, Milan and Beuković, Miloš",
year = "2013",
abstract = "The wild boar (Sus scrofa) is one of most abundant game species in hunting areas of Balkan region. The large fraction of pre-glacial genetic diversity in wild boar populations from the Balkans was addressed due to high proportion of unique mtDNA haplotypes found in Greece, indicating Balkan as main refugial area for wild boars. The aim of the present study is to characterize mitochondrial DNA control region variability in wild boars from different areas in the West Balkan region, in order to evaluate level of genetic variability, to detect unique haplotypes and to infer possible structuring. The total number of 163 individuals from different sampling localities were included in the study. A fragment of the mtDNA control region was amplified and sequenced by standard procedures. Population genetic analyses were performed using several computer packages: BioEdit, ARLEQUIN 3.5.1.2., Network 4.6.0.0 and MEGA5. Eleven different haplotypes were identified and haplotype diversity was 0.676, nucleotide diversity 0.0026, and the average number of nucleotide differences (k) 1.169. The mismatch distribution and neutrality tests indicated the expansion of the all populations. It is shown that high level of genetic diversity is present in the wild boars from the West Balkan region and we have managed to detect regional unique haplotypes in high frequency. Genetic diversity differences have been found in regional wild boar groups, clustering them in two main clusters, but further speculations on the reasons for the observed clustering are prevented due to restricted informativness of the single locus marker. Obtained knowledge of genetic variation in the wild boar may be relevant for improving knowledge of the phylogeny and phylogeography of the wild boars, but as well as for hunting societies and responsible authorities for the effective control of wild boar populations., Divlja svinja (Sus scrofa) je jedna od najbrojnijih vrsta divljači u lovnim područjima Balkana. Na osnovu velikog broja jedinstvenih haplotipova u populacijama divljih svinja Grčke, zaključeno je da se veliki deo pre-glacijalnog genetičkog diverziteta ovih populacija zadržao na Balkanskom poluostrvu, koje je označeno kao glavni refugijum za divlje svinje. Cilj ovog rada je karakterizacija varijabilnosti kontrolnog regiona mitohondrijalne DNK kod divljih svinja sa različitih lokaliteta Zapadnog Balkana, u svrhu utvrđivanja genetičke varijabilnosti, detekcije jedinstvenih haplotipova i definisanja moguće struktuiranosti populacije. Ukupno su analizirane 163 jedinke sa različitih lokaliteta. Deo kontrolnog regiona mtDNK je umnožen i sekvenciran standardnim metodama. Populaciono-genetička analiza urađena je pomoću programskih paketa za analizu sekvenci: BioEdit, ARLEQUIN 3.5.1.2., Network 4.6.0.0 and MEGA5. Nađeno je jedanaest haplotipova i diverzitet haplotipova je iznosio 0.676, diverzitet nukleotida 0.0026, dok je prosečan broj nukleotidnih razlika bio 1.169. Test unimodalne distribucije i testovi neutralnosti pokazali su ekspanziju svih populacija. Pokazan je visok nivo genetičkog diverziteta u populacijama divljih svinja Zapadnog Balkana i detektovani su haplotipovi jedinstveni za regione u visokoj frekvenciji. Razlike u genetičkom diverzitetu pokazane su u regionalnim grupama, i klaster analiza je pokazala prisustvo dve odvojene grane, ali konačan zaključak o stepenu struktuiranosti nije mogao biti donet, zbog ograničene informativnosti jednog genskog markera. Dobijeni rezultati o genetičkoj varijabilnosti divljih svinja relevantni su za rekonstrukciju filogenije i filogeografije ove vrste divljači, ali i za organizacije odgovorne za efektivnu kontrolu populacija divljih svinja.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Mitochondrial DNA control region variability in wild boars from west Balkans, Varijabilnost kontrolnog regiona mitohondrijalne DNK divljih svinja zapadnog Balkana",
volume = "45",
number = "2",
pages = "515-526",
doi = "10.2298/GENSR1302515D"
}

Đan, M., Veličković, N., Obreht, D., Kočiš-Tubić, N., Marković, V., Stevanović, M.,& Beuković, M.. (2013). Mitochondrial DNA control region variability in wild boars from west Balkans. in Genetika
Društvo genetičara Srbije, Beograd., 45(2), 515-526.
https://doi.org/10.2298/GENSR1302515D

Đan M, Veličković N, Obreht D, Kočiš-Tubić N, Marković V, Stevanović M, Beuković M. Mitochondrial DNA control region variability in wild boars from west Balkans. in Genetika. 2013;45(2):515-526.
doi:10.2298/GENSR1302515D .

Đan, Mihajla, Veličković, Nevena, Obreht, Dragana, Kočiš-Tubić, Nataša, Marković, Vladimir, Stevanović, Milan, Beuković, Miloš, "Mitochondrial DNA control region variability in wild boars from west Balkans" in Genetika, 45, no. 2 (2013):515-526,
https://doi.org/10.2298/GENSR1302515D . .

TY  - JOUR
AU  - Stevanović, Milan
AU  - Mladenović Drinić, Snežana
AU  - Dragičević, Vesna
AU  - Čamdžija, Zoran
AU  - Filipović, Milomir
AU  - Veličković, Nevena
AU  - Stanković, Goran
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/439
AB  - The aim of this study was to investigate chemical composition of grain of 20 maize hybrids, from different maturity groups, to define their nutritional quality from the point of main grain constituents: starch, total proteins, soluble proteins, oil, phytate, inorganic phosphorus, and soluble phenolics, as well as mass of 1.000 grains. A set of 20 ZP hybrids, FAO 400-800, were grown in a randomized complete block design (RCBD) at Zemun Polje (Serbia), during the summer of 2009. Average content of proteins, oil and starch of analyzed maize hybrids was 10.5%, 4.7% and 80.8%, respectively. Hybrids from FAO maturity group 600 had highest grain mass (in average 419.5 g). Genetic variability in seed phytate content was observed, with values ranging from 2.64 to 3.75, averaged 3.44 g kg-1. Inorganic phosphorus (Pi) concentrations were between 0.120 µ g g-1 (ZP 805) to 0.415 µ g g-1 (ZP 588ex), averaged 0.24 µ g g-1. Content of soluble proteins varied from 54.33 to 78.74 mg g-1. The high positive correlation was observed between total proteins and oil content in hybrids, as well as between starch and phenolics content. Phytate content was positively correlated with proteins, but this correlation was not significant, while the correlation between phytate and mass of 1,000 grains was positive and significant. A negative correlation occurred between phytate and starch.
AB  - U ovom radu je analizirana hemijska kompozicija zrna 20 hibrida kukuruza različitih FAO grupa zrenja sa ciljem da se definiše njihova nutritivna vrednost na osnovu osnovnih biohemijskih komponenti zrna: skroba, proteina, ulja, fitata neorganskog fosfora, fenola i mase 1000 zrna. Eksperiment je postavljen u u blok dizajnu (RCBD) na eksperimentalnim poljima Instituta za kukuruz u Zemun Polju tokom sezone 2009. Prosečne vrednosti proteina, ulja i skroba analiziranih hibrida kukuruza iznosile su 10.5%, 4.7% and 80.8%, respektivno. Hibridi FAO grupe zrenja 600 imali su najveću masu 1000 zrna (u proseku 419.5 g). Uočena je varijabilnost u sadržaju fitina kod analiziranih hibrida i ona je iznosila od 2.64 do 3.75, sa prosečnom vrednošću od 3.44 g kg-1. Koncentracija neorganskog fosfor (Pi) se kretala od 0.120 µg g-1 (ZP 805) do 0.415 µ g g-1 (ZP 588ex), sa prosekom od 0.24 µ g g-1. Sadržaj solubilnih proteina je varirao od 54.33 do 78.74 mg g-1. Visoko pozitivna korelacija uočena je između sadržaja protein i ulja u zrnu, kao i između skroba i fenola. Fitat je bio u pozitivnoj korelaciji sa proteinima, ali ova korelacija nije bila statistički značajna, dok je korelacija između fitata i mase 1000 zrna pozitivna i signifikantna. Negativna korelacija je uočena između fitata i skroba.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - An assessment of nutritional quality of hybrid maize grain based on chemical composition
T1  - Procena nutritivnog kvaliteta hibrida kukuruza na osnovu hemijske kompozicije zrna
VL  - 44
IS  - 3
SP  - 571
EP  - 582
DO  - 10.2298/GENSR1203571S
ER  - 

@article{
author = "Stevanović, Milan and Mladenović Drinić, Snežana and Dragičević, Vesna and Čamdžija, Zoran and Filipović, Milomir and Veličković, Nevena and Stanković, Goran",
year = "2012",
abstract = "The aim of this study was to investigate chemical composition of grain of 20 maize hybrids, from different maturity groups, to define their nutritional quality from the point of main grain constituents: starch, total proteins, soluble proteins, oil, phytate, inorganic phosphorus, and soluble phenolics, as well as mass of 1.000 grains. A set of 20 ZP hybrids, FAO 400-800, were grown in a randomized complete block design (RCBD) at Zemun Polje (Serbia), during the summer of 2009. Average content of proteins, oil and starch of analyzed maize hybrids was 10.5%, 4.7% and 80.8%, respectively. Hybrids from FAO maturity group 600 had highest grain mass (in average 419.5 g). Genetic variability in seed phytate content was observed, with values ranging from 2.64 to 3.75, averaged 3.44 g kg-1. Inorganic phosphorus (Pi) concentrations were between 0.120 µ g g-1 (ZP 805) to 0.415 µ g g-1 (ZP 588ex), averaged 0.24 µ g g-1. Content of soluble proteins varied from 54.33 to 78.74 mg g-1. The high positive correlation was observed between total proteins and oil content in hybrids, as well as between starch and phenolics content. Phytate content was positively correlated with proteins, but this correlation was not significant, while the correlation between phytate and mass of 1,000 grains was positive and significant. A negative correlation occurred between phytate and starch., U ovom radu je analizirana hemijska kompozicija zrna 20 hibrida kukuruza različitih FAO grupa zrenja sa ciljem da se definiše njihova nutritivna vrednost na osnovu osnovnih biohemijskih komponenti zrna: skroba, proteina, ulja, fitata neorganskog fosfora, fenola i mase 1000 zrna. Eksperiment je postavljen u u blok dizajnu (RCBD) na eksperimentalnim poljima Instituta za kukuruz u Zemun Polju tokom sezone 2009. Prosečne vrednosti proteina, ulja i skroba analiziranih hibrida kukuruza iznosile su 10.5%, 4.7% and 80.8%, respektivno. Hibridi FAO grupe zrenja 600 imali su najveću masu 1000 zrna (u proseku 419.5 g). Uočena je varijabilnost u sadržaju fitina kod analiziranih hibrida i ona je iznosila od 2.64 do 3.75, sa prosečnom vrednošću od 3.44 g kg-1. Koncentracija neorganskog fosfor (Pi) se kretala od 0.120 µg g-1 (ZP 805) do 0.415 µ g g-1 (ZP 588ex), sa prosekom od 0.24 µ g g-1. Sadržaj solubilnih proteina je varirao od 54.33 do 78.74 mg g-1. Visoko pozitivna korelacija uočena je između sadržaja protein i ulja u zrnu, kao i između skroba i fenola. Fitat je bio u pozitivnoj korelaciji sa proteinima, ali ova korelacija nije bila statistički značajna, dok je korelacija između fitata i mase 1000 zrna pozitivna i signifikantna. Negativna korelacija je uočena između fitata i skroba.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "An assessment of nutritional quality of hybrid maize grain based on chemical composition, Procena nutritivnog kvaliteta hibrida kukuruza na osnovu hemijske kompozicije zrna",
volume = "44",
number = "3",
pages = "571-582",
doi = "10.2298/GENSR1203571S"
}

Stevanović, M., Mladenović Drinić, S., Dragičević, V., Čamdžija, Z., Filipović, M., Veličković, N.,& Stanković, G.. (2012). An assessment of nutritional quality of hybrid maize grain based on chemical composition. in Genetika
Društvo genetičara Srbije, Beograd., 44(3), 571-582.
https://doi.org/10.2298/GENSR1203571S

Stevanović M, Mladenović Drinić S, Dragičević V, Čamdžija Z, Filipović M, Veličković N, Stanković G. An assessment of nutritional quality of hybrid maize grain based on chemical composition. in Genetika. 2012;44(3):571-582.
doi:10.2298/GENSR1203571S .

Stevanović, Milan, Mladenović Drinić, Snežana, Dragičević, Vesna, Čamdžija, Zoran, Filipović, Milomir, Veličković, Nevena, Stanković, Goran, "An assessment of nutritional quality of hybrid maize grain based on chemical composition" in Genetika, 44, no. 3 (2012):571-582,
https://doi.org/10.2298/GENSR1203571S . .