Krnjaja, Vesna

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orcid::0000-0002-2126-609X
  • Krnjaja, Vesna (52)
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Author's Bibliography

Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate

Mandić, Violeta; Đorđević, Snežana; Bijelić, Zorica; Krnjaja, Vesna; Pantelić, Vlada; Simić, Aleksandar; Dragičević, Vesna

(Basel : Multidisciplinary Digital Publishing Institute, 2020)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Đorđević, Snežana
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Pantelić, Vlada
AU  - Simić, Aleksandar
AU  - Dragičević, Vesna
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/798
AB  - The main nitrogen (N) sources in soybean production originate from soil fixing bacteria
Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not
su cient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the e ects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha�����1) on quantitative and qualitative parameters and on rain use e ciency (RUE) under contrasting weather conditions in the Pannonian region of Serbia. A field study conducted during two di erent growing seasons: first year with unfavorable weather conditions and second year with favorable weather conditions. As expected, the quantitative parameters, oil content, and RUE were higher in the year with favorable growing season, the second one. According to measured parameters, the genotype Valjevka performed higher yield potential as compared to the genotype Galina. The highest values of quantitative parameters and RUE were recorded at 60 kg N ha�����1, protein content at 90 kg N ha�����1 and oil content 0 kg N ha�����1 (control). This study suggests that proper genotype selection and application of 60 kg N ha�����1 as a starter dose with rhizobial inoculation could contribute to the high yield, while protein could be altered by N amount, independently on genotype.
PB  - Basel : Multidisciplinary Digital Publishing Institute
T2  - Agronomy
T1  - Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate
VL  - 10
IS  - 4
SP  - 535
DO  - 10.3390/agronomy10040535
ER  - 
@article{
author = "Mandić, Violeta and Đorđević, Snežana and Bijelić, Zorica and Krnjaja, Vesna and Pantelić, Vlada and Simić, Aleksandar and Dragičević, Vesna",
year = "2020",
url = "http://rik.mrizp.rs/handle/123456789/798",
abstract = "The main nitrogen (N) sources in soybean production originate from soil fixing bacteria
Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not
su cient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the e ects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha�����1) on quantitative and qualitative parameters and on rain use e ciency (RUE) under contrasting weather conditions in the Pannonian region of Serbia. A field study conducted during two di erent growing seasons: first year with unfavorable weather conditions and second year with favorable weather conditions. As expected, the quantitative parameters, oil content, and RUE were higher in the year with favorable growing season, the second one. According to measured parameters, the genotype Valjevka performed higher yield potential as compared to the genotype Galina. The highest values of quantitative parameters and RUE were recorded at 60 kg N ha�����1, protein content at 90 kg N ha�����1 and oil content 0 kg N ha�����1 (control). This study suggests that proper genotype selection and application of 60 kg N ha�����1 as a starter dose with rhizobial inoculation could contribute to the high yield, while protein could be altered by N amount, independently on genotype.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute",
journal = "Agronomy",
title = "Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate",
volume = "10",
number = "4",
pages = "535",
doi = "10.3390/agronomy10040535"
}
Mandić, V., Đorđević, S., Bijelić, Z., Krnjaja, V., Pantelić, V., Simić, A.,& Dragičević, V. (2020). Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate.
Agronomy
Basel : Multidisciplinary Digital Publishing Institute., 10(4), 535.
https://doi.org/10.3390/agronomy10040535
Mandić V, Đorđević S, Bijelić Z, Krnjaja V, Pantelić V, Simić A, Dragičević V. Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate. Agronomy. 2020;10(4):535
Mandić Violeta, Đorđević Snežana, Bijelić Zorica, Krnjaja Vesna, Pantelić Vlada, Simić Aleksandar, Dragičević Vesna, "Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate" Agronomy, 10, no. 4 (2020):535,
https://doi.org/10.3390/agronomy10040535 .
4
2
4

Sowing and fertilization strategies to improve maize productivity

Mandić, Violeta; Bijelić, Zorica; Krnjaja, Vesna; Simić, Aleksandar; Simić, Milena; Brankov, Milan; Đorđević, Snežana

(Roma : Unità di Ricerca per la Valorizzazione qualitativa dei cereali, 2020)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Simić, Aleksandar
AU  - Simić, Milena
AU  - Brankov, Milan
AU  - Đorđević, Snežana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/817
AB  - Field experiment was conducted to examine the impacts of two sowing dates (8 April - first date of sowing and
21 April - second date of sowing) and four nitrogen rates (0, 60, 120 and 180 kg ha-1) on the productivity of maize hybrid ‘ZP 434’ in the Pannonian region of Serbia during 2016 and 2017 seasons. The dry period during late
vegetative development and grain filling stage in 2017 decreased ear traits, grain yield, starch and oil contents,
nitrogen agronomic (NAE) and nitrogen use efficiency (NUE). The highest number of grains per ear, starch and oil
contents, starch and oil yields and lower rainfall use efficiency (RUE) and protein content were obtained from the
early sowing date. The ear traits, grain yield, RUE, protein content, oil content and yield of starch, protein and oil
significantly increased while NAE, NUE and starch content significantly decreased with increasing nitrogen rate.
The results indicated a significant inverse correlation between starch and protein contents, which prevents the
improvement of these two parameters simultaneously. Thus, timely sowing and nitrogen input should be used as
long term management strategies for increasing maize yield and grain quality.
PB  - Roma : Unità di Ricerca per la Valorizzazione qualitativa dei cereali
T2  - Maydica
T1  - Sowing and fertilization strategies to improve maize productivity
VL  - 65
IS  - 2
SP  - 1
EP  - 9
ER  - 
@article{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Simić, Aleksandar and Simić, Milena and Brankov, Milan and Đorđević, Snežana",
year = "2020",
url = "http://rik.mrizp.rs/handle/123456789/817",
abstract = "Field experiment was conducted to examine the impacts of two sowing dates (8 April - first date of sowing and
21 April - second date of sowing) and four nitrogen rates (0, 60, 120 and 180 kg ha-1) on the productivity of maize hybrid ‘ZP 434’ in the Pannonian region of Serbia during 2016 and 2017 seasons. The dry period during late
vegetative development and grain filling stage in 2017 decreased ear traits, grain yield, starch and oil contents,
nitrogen agronomic (NAE) and nitrogen use efficiency (NUE). The highest number of grains per ear, starch and oil
contents, starch and oil yields and lower rainfall use efficiency (RUE) and protein content were obtained from the
early sowing date. The ear traits, grain yield, RUE, protein content, oil content and yield of starch, protein and oil
significantly increased while NAE, NUE and starch content significantly decreased with increasing nitrogen rate.
The results indicated a significant inverse correlation between starch and protein contents, which prevents the
improvement of these two parameters simultaneously. Thus, timely sowing and nitrogen input should be used as
long term management strategies for increasing maize yield and grain quality.",
publisher = "Roma : Unità di Ricerca per la Valorizzazione qualitativa dei cereali",
journal = "Maydica",
title = "Sowing and fertilization strategies to improve maize productivity",
volume = "65",
number = "2",
pages = "1-9"
}
Mandić, V., Bijelić, Z., Krnjaja, V., Simić, A., Simić, M., Brankov, M.,& Đorđević, S. (2020). Sowing and fertilization strategies to improve maize productivity.
Maydica
Roma : Unità di Ricerca per la Valorizzazione qualitativa dei cereali., 65(2), 1-9.
Mandić V, Bijelić Z, Krnjaja V, Simić A, Simić M, Brankov M, Đorđević S. Sowing and fertilization strategies to improve maize productivity. Maydica. 2020;65(2):1-9
Mandić Violeta, Bijelić Zorica, Krnjaja Vesna, Simić Aleksandar, Simić Milena, Brankov Milan, Đorđević Snežana, "Sowing and fertilization strategies to improve maize productivity" Maydica, 65, no. 2 (2020):1-9

Mycobiota and aflatoxin B1 in poultry feeds

Krnjaja, Vesna; Petrović, Tanja; Stanković, Slavica; Lukić, Miloš; Škrbić, Zdenka; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Škrbić, Zdenka
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/742
AB  - In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.
AB  - U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Mycobiota and aflatoxin B1 in poultry feeds
T1  - Mikobiota i aflatoksin B1 u hrani za živinu
VL  - 35
IS  - 1
SP  - 61
EP  - 69
DO  - 10.2298/BAH1901061K
ER  - 
@article{
author = "Krnjaja, Vesna and Petrović, Tanja and Stanković, Slavica and Lukić, Miloš and Škrbić, Zdenka and Mandić, Violeta and Bijelić, Zorica",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/742",
abstract = "In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain., U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Mycobiota and aflatoxin B1 in poultry feeds, Mikobiota i aflatoksin B1 u hrani za živinu",
volume = "35",
number = "1",
pages = "61-69",
doi = "10.2298/BAH1901061K"
}
Krnjaja, V., Petrović, T., Stanković, S., Lukić, M., Škrbić, Z., Mandić, V.,& Bijelić, Z. (2019). Mikobiota i aflatoksin B1 u hrani za živinu.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 35(1), 61-69.
https://doi.org/10.2298/BAH1901061K
Krnjaja V, Petrović T, Stanković S, Lukić M, Škrbić Z, Mandić V, Bijelić Z. Mikobiota i aflatoksin B1 u hrani za živinu. Biotechnology in Animal Husbandry. 2019;35(1):61-69
Krnjaja Vesna, Petrović Tanja, Stanković Slavica, Lukić Miloš, Škrbić Zdenka, Mandić Violeta, Bijelić Zorica, "Mikobiota i aflatoksin B1 u hrani za živinu" Biotechnology in Animal Husbandry, 35, no. 1 (2019):61-69,
https://doi.org/10.2298/BAH1901061K .

Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains

Krnjaja, Vesna; Mandić, Violeta; Lukić, Miloš; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Vasić, Tanja
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/735
AB  - Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains
VL  - 116
SP  - 126
EP  - 131
DO  - 10.1016/j.cropro.2018.10.021
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lukić, Miloš and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Vasić, Tanja",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/735",
abstract = "Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains",
volume = "116",
pages = "126-131",
doi = "10.1016/j.cropro.2018.10.021"
}
Krnjaja, V., Mandić, V., Lukić, M., Bijelić, Z., Stanković, S., Obradović, A.,& Vasić, T. (2019). Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains.
Crop Protection
Elsevier Sci Ltd, Oxford., 116, 126-131.
https://doi.org/10.1016/j.cropro.2018.10.021
Krnjaja V, Mandić V, Lukić M, Bijelić Z, Stanković S, Obradović A, Vasić T. Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains. Crop Protection. 2019;116:126-131
Krnjaja Vesna, Mandić Violeta, Lukić Miloš, Bijelić Zorica, Stanković Slavica, Obradović Ana, Vasić Tanja, "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains" Crop Protection, 116 (2019):126-131,
https://doi.org/10.1016/j.cropro.2018.10.021 .
7
3
5

Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence

Obradović, Ana; Krnjaja, Vesna; Nikolić, Milica; Delibašić, Goran; Filipović, Milomir; Stanković, Goran; Stanković, Slavica

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Nikolić, Milica
AU  - Delibašić, Goran
AU  - Filipović, Milomir
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/738
AB  - Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species.
AB  - Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence
T1  - Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava
VL  - 34
IS  - 4
SP  - 469
EP  - 480
DO  - 10.2298/BAH1804469O
ER  - 
@article{
author = "Obradović, Ana and Krnjaja, Vesna and Nikolić, Milica and Delibašić, Goran and Filipović, Milomir and Stanković, Goran and Stanković, Slavica",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/738",
abstract = "Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species., Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence, Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava",
volume = "34",
number = "4",
pages = "469-480",
doi = "10.2298/BAH1804469O"
}
Obradović, A., Krnjaja, V., Nikolić, M., Delibašić, G., Filipović, M., Stanković, G.,& Stanković, S. (2018). Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(4), 469-480.
https://doi.org/10.2298/BAH1804469O
Obradović A, Krnjaja V, Nikolić M, Delibašić G, Filipović M, Stanković G, Stanković S. Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava. Biotechnology in Animal Husbandry. 2018;34(4):469-480
Obradović Ana, Krnjaja Vesna, Nikolić Milica, Delibašić Goran, Filipović Milomir, Stanković Goran, Stanković Slavica, "Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava" Biotechnology in Animal Husbandry, 34, no. 4 (2018):469-480,
https://doi.org/10.2298/BAH1804469O .
4

Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia

Krnjaja, Vesna; Mandić, Violeta; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Bozić, Manja

(Mdpi, Basel, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Bozić, Manja
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/702
AB  - Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.
PB  - Mdpi, Basel
T2  - Toxins
T1  - Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia
VL  - 10
IS  - 11
DO  - 10.3390/toxins10110460
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Bozić, Manja",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/702",
abstract = "Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.",
publisher = "Mdpi, Basel",
journal = "Toxins",
title = "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia",
volume = "10",
number = "11",
doi = "10.3390/toxins10110460"
}
Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T.,& Bozić, M. (2018). Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia.
Toxins
Mdpi, Basel., 10(11).
https://doi.org/10.3390/toxins10110460
Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Bozić M. Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. Toxins. 2018;10(11)
Krnjaja Vesna, Mandić Violeta, Bijelić Zorica, Stanković Slavica, Obradović Ana, Petrović Tanja, Bozić Manja, "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia" Toxins, 10, no. 11 (2018),
https://doi.org/10.3390/toxins10110460 .
3
1
2

Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia

Krnjaja, Vesna; Stanković, Slavica; Lukić, Miloš; Mićić, Nenad; Petrović, Tanja; Bijelić, Zorica; Mandić, Violeta

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Mićić, Nenad
AU  - Petrović, Tanja
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/720
AB  - This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.
AB  - Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia
T1  - Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji
VL  - 34
IS  - 2
SP  - 239
EP  - 249
DO  - 10.2298/bah1802239K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Lukić, Miloš and Mićić, Nenad and Petrović, Tanja and Bijelić, Zorica and Mandić, Violeta",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/720",
abstract = "This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain., Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia, Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji",
volume = "34",
number = "2",
pages = "239-249",
doi = "10.2298/bah1802239K"
}
Krnjaja, V., Stanković, S., Lukić, M., Mićić, N., Petrović, T., Bijelić, Z.,& Mandić, V. (2018). Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(2), 239-249.
https://doi.org/10.2298/bah1802239K
Krnjaja V, Stanković S, Lukić M, Mićić N, Petrović T, Bijelić Z, Mandić V. Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji. Biotechnology in Animal Husbandry. 2018;34(2):239-249
Krnjaja Vesna, Stanković Slavica, Lukić Miloš, Mićić Nenad, Petrović Tanja, Bijelić Zorica, Mandić Violeta, "Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji" Biotechnology in Animal Husbandry, 34, no. 2 (2018):239-249,
https://doi.org/10.2298/bah1802239K .
1

Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia

Obradović, Ana; Stanković, Slavica; Nikolić, Ana; Ignjatović-Micić, Dragana; Krnjaja, Vesna; Stepanović, Jelena; Duduk, Bojan

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Krnjaja, Vesna
AU  - Stepanović, Jelena
AU  - Duduk, Bojan
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/682
AB  - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia
VL  - 49
IS  - 1
SP  - 355
EP  - 364
DO  - 10.2298/GENSR1701355O
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Nikolić, Ana and Ignjatović-Micić, Dragana and Krnjaja, Vesna and Stepanović, Jelena and Duduk, Bojan",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/682",
abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia",
volume = "49",
number = "1",
pages = "355-364",
doi = "10.2298/GENSR1701355O"
}
Obradović, A., Stanković, S., Nikolić, A., Ignjatović-Micić, D., Krnjaja, V., Stepanović, J.,& Duduk, B. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia.
Genetika
Društvo genetičara Srbije, Beograd., 49(1), 355-364.
https://doi.org/10.2298/GENSR1701355O
Obradović A, Stanković S, Nikolić A, Ignjatović-Micić D, Krnjaja V, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. Genetika. 2017;49(1):355-364
Obradović Ana, Stanković Slavica, Nikolić Ana, Ignjatović-Micić Dragana, Krnjaja Vesna, Stepanović Jelena, Duduk Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" Genetika, 49, no. 1 (2017):355-364,
https://doi.org/10.2298/GENSR1701355O .
4
3
3

The rainfall use efficiency and soybean grain yield under rainfed conditions in Vojvodina

Mandić, Violeta; Bijelić, Zorica; Krnjaja, Vesna; Simić, Aleksandar; Ružić-Muslić, Dragana; Dragičević, Vesna; Petričević, Veselin

(Institut za stočarstvo, Beograd, 2017)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Simić, Aleksandar
AU  - Ružić-Muslić, Dragana
AU  - Dragičević, Vesna
AU  - Petričević, Veselin
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/667
AB  - Rainfall is one of the most important environmental factors influencing crop production under dry land farming conditions. In the Republic of Serbia, the soybean is produced under rainfed conditions, and therefore online monitoring of the rainfall use efficiency (RUE) is essential for efficient management of production. The research aim was to estimate the effects of amount rainfall during the growing season (RGS) and average monthly rainfall on soybean grain yield (GY) in the Vojvodina during the sixteen year period (2000-2015). Distributions of RGS were not satisfactory and negatively influenced the expression genetic yield potential of cultivars. Rainfall deficits during the growing season limited the soybean plant reproductive growth stages leading to GY loss. The coefficient of variation indicated that RGS and monthly rainfall changed moderately from year to year. Regression equations showed that GY tended to increase with the amount of rainfall. GY had strong positive relationship with RGS and rainfall in May, July and August. Since the amount and distribution of rainfall during growing season are critical determinants of GY, soybean cultivars of shorter vegetation periods should be developed and cultivated so that maximum utilization of rainfall is ensured.
AB  - Padavine su jedan od najvažnijih faktora spoljašnje sredine koji utiče na produkciju useva u uslovima suvog ratarenja. U Republici Srbiji, soja se proizvodi u uslovima prirodnog vodnog režima, pa je praćenje efikasnosti korišćenja padavina (RUE) neophodno za efikasno upravljanje proizvodnjom. Cilj istraživanja bio je da se proceni efekat količine padavina tokom vegetacionog perioda i prosečnih mesečnih padavina na prinos zrna soje u Vojvodini tokom šestnaestogodišnjeg perioda (2000-2015). Distribucija padavina tokom vegetacionog perioda nije bila zadovoljavajuća i nepovoljno je uticala na ekspresiju genetičkog potencijala rodnosti sorti. Deficit padavina tokom vegetacionog perioda soje ograničile su faze reproduktivnog razvoja i dovele do redukcije prinosa. Koeficijenti varijacije pokazuju da se količina padavina tokom vegetacionog perioda i srednja mesečna količina padavina umereno menjaju iz godine u godinu. Regresijske jednačine su pokazale da se prinos zrna povećavao sa količinom padavina. Prinos zrna je u jakoj pozitivnoj korelaciji sa količinom padavina tokom vegetacionog perioda, u maju, julu i avgustu. S obzirom da su količina i raspored padavina u toku vegetacionog perioda kritična determinanta za prinos zrna, treba razvijati i gajiti sorte soje sa kraćim vegetacionim periodom da bi se obezbedila maksimalna iskorišćenost padavina.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - The rainfall use efficiency and soybean grain yield under rainfed conditions in Vojvodina
T1  - Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini
VL  - 33
IS  - 4
SP  - 475
EP  - 486
DO  - 10.2298/BAH1704475M
ER  - 
@article{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Simić, Aleksandar and Ružić-Muslić, Dragana and Dragičević, Vesna and Petričević, Veselin",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/667",
abstract = "Rainfall is one of the most important environmental factors influencing crop production under dry land farming conditions. In the Republic of Serbia, the soybean is produced under rainfed conditions, and therefore online monitoring of the rainfall use efficiency (RUE) is essential for efficient management of production. The research aim was to estimate the effects of amount rainfall during the growing season (RGS) and average monthly rainfall on soybean grain yield (GY) in the Vojvodina during the sixteen year period (2000-2015). Distributions of RGS were not satisfactory and negatively influenced the expression genetic yield potential of cultivars. Rainfall deficits during the growing season limited the soybean plant reproductive growth stages leading to GY loss. The coefficient of variation indicated that RGS and monthly rainfall changed moderately from year to year. Regression equations showed that GY tended to increase with the amount of rainfall. GY had strong positive relationship with RGS and rainfall in May, July and August. Since the amount and distribution of rainfall during growing season are critical determinants of GY, soybean cultivars of shorter vegetation periods should be developed and cultivated so that maximum utilization of rainfall is ensured., Padavine su jedan od najvažnijih faktora spoljašnje sredine koji utiče na produkciju useva u uslovima suvog ratarenja. U Republici Srbiji, soja se proizvodi u uslovima prirodnog vodnog režima, pa je praćenje efikasnosti korišćenja padavina (RUE) neophodno za efikasno upravljanje proizvodnjom. Cilj istraživanja bio je da se proceni efekat količine padavina tokom vegetacionog perioda i prosečnih mesečnih padavina na prinos zrna soje u Vojvodini tokom šestnaestogodišnjeg perioda (2000-2015). Distribucija padavina tokom vegetacionog perioda nije bila zadovoljavajuća i nepovoljno je uticala na ekspresiju genetičkog potencijala rodnosti sorti. Deficit padavina tokom vegetacionog perioda soje ograničile su faze reproduktivnog razvoja i dovele do redukcije prinosa. Koeficijenti varijacije pokazuju da se količina padavina tokom vegetacionog perioda i srednja mesečna količina padavina umereno menjaju iz godine u godinu. Regresijske jednačine su pokazale da se prinos zrna povećavao sa količinom padavina. Prinos zrna je u jakoj pozitivnoj korelaciji sa količinom padavina tokom vegetacionog perioda, u maju, julu i avgustu. S obzirom da su količina i raspored padavina u toku vegetacionog perioda kritična determinanta za prinos zrna, treba razvijati i gajiti sorte soje sa kraćim vegetacionim periodom da bi se obezbedila maksimalna iskorišćenost padavina.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "The rainfall use efficiency and soybean grain yield under rainfed conditions in Vojvodina, Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini",
volume = "33",
number = "4",
pages = "475-486",
doi = "10.2298/BAH1704475M"
}
Mandić, V., Bijelić, Z., Krnjaja, V., Simić, A., Ružić-Muslić, D., Dragičević, V.,& Petričević, V. (2017). Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 33(4), 475-486.
https://doi.org/10.2298/BAH1704475M
Mandić V, Bijelić Z, Krnjaja V, Simić A, Ružić-Muslić D, Dragičević V, Petričević V. Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini. Biotechnology in Animal Husbandry. 2017;33(4):475-486
Mandić Violeta, Bijelić Zorica, Krnjaja Vesna, Simić Aleksandar, Ružić-Muslić Dragana, Dragičević Vesna, Petričević Veselin, "Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini" Biotechnology in Animal Husbandry, 33, no. 4 (2017):475-486,
https://doi.org/10.2298/BAH1704475M .
5

Fungal contamination of maize grain samples with a special focus on toxigenic genera

Krnjaja, Vesna; Stanojković, Aleksandar; Stanković, Slavica; Lukić, Miloš; Bijelić, Zorica; Mandić, Violeta; Mićić, Nenad

(Institut za stočarstvo, Beograd, 2017)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanojković, Aleksandar
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Mićić, Nenad
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/664
AB  - In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi.
AB  - Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal contamination of maize grain samples with a special focus on toxigenic genera
T1  - Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove
VL  - 33
IS  - 2
SP  - 233
EP  - 241
DO  - 10.2298/BAH1702233K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanojković, Aleksandar and Stanković, Slavica and Lukić, Miloš and Bijelić, Zorica and Mandić, Violeta and Mićić, Nenad",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/664",
abstract = "In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi., Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal contamination of maize grain samples with a special focus on toxigenic genera, Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove",
volume = "33",
number = "2",
pages = "233-241",
doi = "10.2298/BAH1702233K"
}
Krnjaja, V., Stanojković, A., Stanković, S., Lukić, M., Bijelić, Z., Mandić, V.,& Mićić, N. (2017). Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 33(2), 233-241.
https://doi.org/10.2298/BAH1702233K
Krnjaja V, Stanojković A, Stanković S, Lukić M, Bijelić Z, Mandić V, Mićić N. Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove. Biotechnology in Animal Husbandry. 2017;33(2):233-241
Krnjaja Vesna, Stanojković Aleksandar, Stanković Slavica, Lukić Miloš, Bijelić Zorica, Mandić Violeta, Mićić Nenad, "Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove" Biotechnology in Animal Husbandry, 33, no. 2 (2017):233-241,
https://doi.org/10.2298/BAH1702233K .
4

Presence of deoxynivalenol in wheat milling products in Serbia during 2016-2017

Jauković, Marko; Zečević, Veselinka; Stanković, Slavica; Krnjaja, Vesna

(Matica srpska, Novi Sad, 2017)

TY  - JOUR
AU  - Jauković, Marko
AU  - Zečević, Veselinka
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/683
AB  - Deoxynivalenol (DON) is one of several mycotoxins produced by certain Fusarium species that frequently infect wheat, corn, rice, oats, barley and other grains in the field or during storage. DON affects animal and human health causing vomiting, acute temporary nausea, diarrhea, abdominal pain, headache, dizziness and fever. The objective of this study was to evaluate the natural occurrence of deoxynivalenol (DON) in white wheat flour, whole wheat flour and wheat bran. In this study, a total of 75 white wheat flour, whole wheat flour and wheat bran samples were collected in the period of 2016-2017. All samples were analyzed for DON by enzyme-linked immunosorbent assay. DON was detected in 23 out of 45 white wheat flour samples (51.11%), at levels ranging from 99 µg/kg to 440 µg/kg. Out of 15 whole wheat flour samples, 14 were contaminated by DON (93.33%), at levels ranging from 98 µg/kg to 479 µg/kg. The maximum contamination level of DON (2,790 µg/kg) in this study was found in wheat bran. Presence of DON was detected in all 15 samples of wheat bran (100%). These results suggest a high percentage of contaminated samples, especially among wheat bran samples, which raises a risk for consumers of wheat bran and the need to monitor final products before consumption.
AB  - Deoksinivalenol (DON) jedan je od nekoliko mikotoksina koje produkuju određene vrste roda Fusarium, koje često kontaminiraju pšenicu, kukuruz, pirinač, ovas, ječam i ostale žitarice, kako u polju, tako i tokom perioda skladištenja. DON utiče na zdravlje ljudi i životinja izazivajući povraćanje, akutne mučnine, dijareju, abdominalni bol, glavobolje, vrtoglavice i groznicu. Cilj ovog istraživanja bio je da se ispita i proceni prirodna pojava deoksini­valenola (DON) u belom pšeničnom brašnu, integralnom pšeničnom brašnu i pšeničnim mekinjama. Ukupno 75 uzoraka belog pšeničnog brašna, integralnog pšeničnog brašna i pšeničnih mekinja uzorkovano je u periodu 2016-2017. Svi uzorci su analizirani na prisustvo DON-a imunoenzimskim ELISA testovima. Prisustvo DON-a je detektovano u 23 od 45 uzoraka belog pšeničnog brašna (51,11%), u koncentracionom opsegu od 99 do 440 µg/kg. Od 15 ispitanih uzoraka integralnog pšeničnog brašna 14 je bilo kontaminirano (93,33%), a koncentracija DON-a kretala se u opsegu od 98 do 479 µg/kg. Maksimalna koncentracija DON-a (2.790 μg/kg) u ovom istraživanju zabeležena je kod pšeničnih mekinja. Prisustvo DON-a detektovano je u svih 15 ispitivanih uzorka pšeničnih mekinja (100%). Ovi rezultati ukazuju na visok procenat kontaminiranih uzoraka, pogotovo kod uzoraka pšeničnih mekinja, što predstavlja rizik po potrošače i iziskuje potrebu za monitoringom finalnih proizvoda pre puštanja u promet.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Presence of deoxynivalenol in wheat milling products in Serbia during 2016-2017
T1  - Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji
IS  - 133
SP  - 57
EP  - 62
DO  - 10.2298/ZMSPN1733057J
ER  - 
@article{
author = "Jauković, Marko and Zečević, Veselinka and Stanković, Slavica and Krnjaja, Vesna",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/683",
abstract = "Deoxynivalenol (DON) is one of several mycotoxins produced by certain Fusarium species that frequently infect wheat, corn, rice, oats, barley and other grains in the field or during storage. DON affects animal and human health causing vomiting, acute temporary nausea, diarrhea, abdominal pain, headache, dizziness and fever. The objective of this study was to evaluate the natural occurrence of deoxynivalenol (DON) in white wheat flour, whole wheat flour and wheat bran. In this study, a total of 75 white wheat flour, whole wheat flour and wheat bran samples were collected in the period of 2016-2017. All samples were analyzed for DON by enzyme-linked immunosorbent assay. DON was detected in 23 out of 45 white wheat flour samples (51.11%), at levels ranging from 99 µg/kg to 440 µg/kg. Out of 15 whole wheat flour samples, 14 were contaminated by DON (93.33%), at levels ranging from 98 µg/kg to 479 µg/kg. The maximum contamination level of DON (2,790 µg/kg) in this study was found in wheat bran. Presence of DON was detected in all 15 samples of wheat bran (100%). These results suggest a high percentage of contaminated samples, especially among wheat bran samples, which raises a risk for consumers of wheat bran and the need to monitor final products before consumption., Deoksinivalenol (DON) jedan je od nekoliko mikotoksina koje produkuju određene vrste roda Fusarium, koje često kontaminiraju pšenicu, kukuruz, pirinač, ovas, ječam i ostale žitarice, kako u polju, tako i tokom perioda skladištenja. DON utiče na zdravlje ljudi i životinja izazivajući povraćanje, akutne mučnine, dijareju, abdominalni bol, glavobolje, vrtoglavice i groznicu. Cilj ovog istraživanja bio je da se ispita i proceni prirodna pojava deoksini­valenola (DON) u belom pšeničnom brašnu, integralnom pšeničnom brašnu i pšeničnim mekinjama. Ukupno 75 uzoraka belog pšeničnog brašna, integralnog pšeničnog brašna i pšeničnih mekinja uzorkovano je u periodu 2016-2017. Svi uzorci su analizirani na prisustvo DON-a imunoenzimskim ELISA testovima. Prisustvo DON-a je detektovano u 23 od 45 uzoraka belog pšeničnog brašna (51,11%), u koncentracionom opsegu od 99 do 440 µg/kg. Od 15 ispitanih uzoraka integralnog pšeničnog brašna 14 je bilo kontaminirano (93,33%), a koncentracija DON-a kretala se u opsegu od 98 do 479 µg/kg. Maksimalna koncentracija DON-a (2.790 μg/kg) u ovom istraživanju zabeležena je kod pšeničnih mekinja. Prisustvo DON-a detektovano je u svih 15 ispitivanih uzorka pšeničnih mekinja (100%). Ovi rezultati ukazuju na visok procenat kontaminiranih uzoraka, pogotovo kod uzoraka pšeničnih mekinja, što predstavlja rizik po potrošače i iziskuje potrebu za monitoringom finalnih proizvoda pre puštanja u promet.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Presence of deoxynivalenol in wheat milling products in Serbia during 2016-2017, Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji",
number = "133",
pages = "57-62",
doi = "10.2298/ZMSPN1733057J"
}
Jauković, M., Zečević, V., Stanković, S.,& Krnjaja, V. (2017). Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji.
Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(133), 57-62.
https://doi.org/10.2298/ZMSPN1733057J
Jauković M, Zečević V, Stanković S, Krnjaja V. Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji. Zbornik Matice srpske za prirodne nauke. 2017;(133):57-62
Jauković Marko, Zečević Veselinka, Stanković Slavica, Krnjaja Vesna, "Pojava deoksinivalenola u mlinskim proizvodima od pšenice u periodu 2016-2017. u Srbiji" Zbornik Matice srpske za prirodne nauke, no. 133 (2017):57-62,
https://doi.org/10.2298/ZMSPN1733057J .

Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia

Obradović, Ana; Stanković, Slavica; Stevanović, Milan; Krnjaja, Vesna; Bulajić, Aleksandra; Delibašić, Goran

(Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Stevanović, Milan
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Delibašić, Goran
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/665
AB  - Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum.
AB  - Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia
T1  - Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji
VL  - 45
IS  - 3
SP  - 277
EP  - 286
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Stevanović, Milan and Krnjaja, Vesna and Bulajić, Aleksandra and Delibašić, Goran",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/665",
abstract = "Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum., Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia, Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji",
volume = "45",
number = "3",
pages = "277-286"
}
Obradović, A., Stanković, S., Stevanović, M., Krnjaja, V., Bulajić, A.,& Delibašić, G. (2017). Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji.
Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(3), 277-286.
Obradović A, Stanković S, Stevanović M, Krnjaja V, Bulajić A, Delibašić G. Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji. Biljni lekar. 2017;45(3):277-286
Obradović Ana, Stanković Slavica, Stevanović Milan, Krnjaja Vesna, Bulajić Aleksandra, Delibašić Goran, "Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji" Biljni lekar, 45, no. 3 (2017):277-286

Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest

Bijelić, Zorica; Krnjaja, Vesna; Muslic-Ruzić, Dragana; Mandić, Violeta; Škrbić, Zdenka; Lukić, Miloš; Stanković, Slavica

(Ars Docendi, Bucharest, 2017)

TY  - JOUR
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Muslic-Ruzić, Dragana
AU  - Mandić, Violeta
AU  - Škrbić, Zdenka
AU  - Lukić, Miloš
AU  - Stanković, Slavica
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/688
AB  - The aim of this study was to determine the presence of fungi and concentrations of mycotoxins, zearalenone, fumonisin and deoxynivalenol, in lucerne and grass-legume silages, under the influence of different doses of N fertilization and harvesting at different stages of phenological development of the plants. Studies included pure lucerne crop and mixtures of lucerne with cocksfoot, tall fescue and sainfoin, sown in different ratios, fertilized with 0, 70, 140 or 210 kgN ha(-1) and harvested in the butonization phase or at 50% flowering of lucerne plants. Results showed that the total fungi count in the silage depended on the three investigated factors. The highest total fungi count was determined in the mixture silages of lucerne, cocksfoot and tall fescue, which was well treated with different nitrogen quantities, harvested and prepared in later stages of plant development. The most commonly-occurring fungi were Fusarium species (85.5% of fungi were this genus). Of the studied mycotoxins, only DON depended on fertilization, as it reduced the concentration of DON from 0.15 to 0.07 mg kg(-1). Preventing appearance of fungi and their mycotoxins in forage and silage should begin in the field as well as during the process of preparation of silage.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest
VL  - 22
IS  - 5
SP  - 12907
EP  - 12914
ER  - 
@article{
author = "Bijelić, Zorica and Krnjaja, Vesna and Muslic-Ruzić, Dragana and Mandić, Violeta and Škrbić, Zdenka and Lukić, Miloš and Stanković, Slavica",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/688",
abstract = "The aim of this study was to determine the presence of fungi and concentrations of mycotoxins, zearalenone, fumonisin and deoxynivalenol, in lucerne and grass-legume silages, under the influence of different doses of N fertilization and harvesting at different stages of phenological development of the plants. Studies included pure lucerne crop and mixtures of lucerne with cocksfoot, tall fescue and sainfoin, sown in different ratios, fertilized with 0, 70, 140 or 210 kgN ha(-1) and harvested in the butonization phase or at 50% flowering of lucerne plants. Results showed that the total fungi count in the silage depended on the three investigated factors. The highest total fungi count was determined in the mixture silages of lucerne, cocksfoot and tall fescue, which was well treated with different nitrogen quantities, harvested and prepared in later stages of plant development. The most commonly-occurring fungi were Fusarium species (85.5% of fungi were this genus). Of the studied mycotoxins, only DON depended on fertilization, as it reduced the concentration of DON from 0.15 to 0.07 mg kg(-1). Preventing appearance of fungi and their mycotoxins in forage and silage should begin in the field as well as during the process of preparation of silage.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest",
volume = "22",
number = "5",
pages = "12907-12914"
}
Bijelić, Z., Krnjaja, V., Muslic-Ruzić, D., Mandić, V., Škrbić, Z., Lukić, M.,& Stanković, S. (2017). Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest.
Romanian Biotechnological Letters
Ars Docendi, Bucharest., 22(5), 12907-12914.
Bijelić Z, Krnjaja V, Muslic-Ruzić D, Mandić V, Škrbić Z, Lukić M, Stanković S. Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest. Romanian Biotechnological Letters. 2017;22(5):12907-12914
Bijelić Zorica, Krnjaja Vesna, Muslic-Ruzić Dragana, Mandić Violeta, Škrbić Zdenka, Lukić Miloš, Stanković Slavica, "Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest" Romanian Biotechnological Letters, 22, no. 5 (2017):12907-12914
1

Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt

Jauković, Marko; Zečević, Veselinka; Stanković, Slavica; Krnjaja, Vesna; Nikić, Tanja S.; Bailović, Stanislava M.; Tadić, Jelena I.

(Amer Soc Brewing Chemists Inc, St Paul, 2017)

TY  - JOUR
AU  - Jauković, Marko
AU  - Zečević, Veselinka
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Nikić, Tanja S.
AU  - Bailović, Stanislava M.
AU  - Tadić, Jelena I.
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/687
AB  - The primary aim of this research was to investigate the effect of dilute alkaline steeping on molds and toxicity of maize malt. Samples of maize were collected from one farm located in the Serbian autonomous region Vojvodina. Steeping regimes were performed by using 0.1, 0.2, and 0.3% NaOH solutions. The effects of these solutions on mold contamination, total aflatoxin (AFLA), deoxynivalenol (DON), zearalenone (ZON), rootlet length, moisture, total nitrogen, content of protein, fat content, ash content, content of total carbohydrates, and energy value of maize malt were evaluated. Steeping in 0.1, 0.2, and 0.3% NaOH reduced the levels of molds from 10(6) to 10(4) CFU/g, and steeping in 0.3% NaOH eliminated some of the mold genera. Also, it significantly reduced (P  lt  0.05) the level of total AFLA, DON, and ZON. However, steeping in (control) water also significantly reduced (P  lt  0.05) the level of DON and ZON. Malting also significantly reduced (P  lt  0.05) total nitrogen and therefore content of proteins. However, steeping in 0.1, 0.2, and 0.3% NaOH caused significantly smaller reductions (P  lt  0.05). Significant reduction (P  lt  0.05) was also observed in the rootlet length, content of fat, and ash content. Steeping in 0.3% NaOH is proposed as a method for the reduction of mold and AFLA, DON, and ZON contamination during maize malting. Energy value of maize increases in the malting process regardless of the steeping regime.
PB  - Amer Soc Brewing Chemists Inc, St Paul
T2  - Journal of the American Society of Brewing Chemists
T1  - Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt
VL  - 75
IS  - 4
SP  - 369
EP  - 373
DO  - 10.1094/ASBCJ-2017-4043-01
ER  - 
@article{
author = "Jauković, Marko and Zečević, Veselinka and Stanković, Slavica and Krnjaja, Vesna and Nikić, Tanja S. and Bailović, Stanislava M. and Tadić, Jelena I.",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/687",
abstract = "The primary aim of this research was to investigate the effect of dilute alkaline steeping on molds and toxicity of maize malt. Samples of maize were collected from one farm located in the Serbian autonomous region Vojvodina. Steeping regimes were performed by using 0.1, 0.2, and 0.3% NaOH solutions. The effects of these solutions on mold contamination, total aflatoxin (AFLA), deoxynivalenol (DON), zearalenone (ZON), rootlet length, moisture, total nitrogen, content of protein, fat content, ash content, content of total carbohydrates, and energy value of maize malt were evaluated. Steeping in 0.1, 0.2, and 0.3% NaOH reduced the levels of molds from 10(6) to 10(4) CFU/g, and steeping in 0.3% NaOH eliminated some of the mold genera. Also, it significantly reduced (P  lt  0.05) the level of total AFLA, DON, and ZON. However, steeping in (control) water also significantly reduced (P  lt  0.05) the level of DON and ZON. Malting also significantly reduced (P  lt  0.05) total nitrogen and therefore content of proteins. However, steeping in 0.1, 0.2, and 0.3% NaOH caused significantly smaller reductions (P  lt  0.05). Significant reduction (P  lt  0.05) was also observed in the rootlet length, content of fat, and ash content. Steeping in 0.3% NaOH is proposed as a method for the reduction of mold and AFLA, DON, and ZON contamination during maize malting. Energy value of maize increases in the malting process regardless of the steeping regime.",
publisher = "Amer Soc Brewing Chemists Inc, St Paul",
journal = "Journal of the American Society of Brewing Chemists",
title = "Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt",
volume = "75",
number = "4",
pages = "369-373",
doi = "10.1094/ASBCJ-2017-4043-01"
}
Jauković, M., Zečević, V., Stanković, S., Krnjaja, V., Nikić, T. S., Bailović, S. M.,& Tadić, J. I. (2017). Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt.
Journal of the American Society of Brewing Chemists
Amer Soc Brewing Chemists Inc, St Paul., 75(4), 369-373.
https://doi.org/10.1094/ASBCJ-2017-4043-01
Jauković M, Zečević V, Stanković S, Krnjaja V, Nikić TS, Bailović SM, Tadić JI. Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt. Journal of the American Society of Brewing Chemists. 2017;75(4):369-373
Jauković Marko, Zečević Veselinka, Stanković Slavica, Krnjaja Vesna, Nikić Tanja S., Bailović Stanislava M., Tadić Jelena I., "Effect of Dilute Alkaline Steeping on Mold Contamination, Toxicity, and Nutritive Value of Maize Malt" Journal of the American Society of Brewing Chemists, 75, no. 4 (2017):369-373,
https://doi.org/10.1094/ASBCJ-2017-4043-01 .
1
1
1

Fungal and mycotoxin contamination of maize hybrids in different maturity groups

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Bijelić, Zorica; Vasić, Tanja; Jauković, Marko

(Institut za stočarstvo, Beograd, 2016)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Vasić, Tanja
AU  - Jauković, Marko
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/627
AB  - In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods.
AB  - U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal and mycotoxin contamination of maize hybrids in different maturity groups
T1  - Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja
VL  - 32
IS  - 1
SP  - 71
EP  - 81
DO  - 10.2298/BAH1601071K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Vasić, Tanja and Jauković, Marko",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/627",
abstract = "In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods., U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal and mycotoxin contamination of maize hybrids in different maturity groups, Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja",
volume = "32",
number = "1",
pages = "71-81",
doi = "10.2298/BAH1601071K"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Bijelić, Z., Vasić, T.,& Jauković, M. (2016). Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(1), 71-81.
https://doi.org/10.2298/BAH1601071K
Krnjaja V, Stanković S, Obradović A, Mandić V, Bijelić Z, Vasić T, Jauković M. Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja. Biotechnology in Animal Husbandry. 2016;32(1):71-81
Krnjaja Vesna, Stanković Slavica, Obradović Ana, Mandić Violeta, Bijelić Zorica, Vasić Tanja, Jauković Marko, "Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja" Biotechnology in Animal Husbandry, 32, no. 1 (2016):71-81,
https://doi.org/10.2298/BAH1601071K .
4

Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover

Vasić, Tanja; Terzić, Dragan; Milenković, Jasmina; Marković, Jordan; Krnjaja, Vesna; Jevremović, Darko; Stanković, Slavica

(Društvo genetičara Srbije, Beograd, 2016)

TY  - JOUR
AU  - Vasić, Tanja
AU  - Terzić, Dragan
AU  - Milenković, Jasmina
AU  - Marković, Jordan
AU  - Krnjaja, Vesna
AU  - Jevremović, Darko
AU  - Stanković, Slavica
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/650
AB  - A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover
VL  - 48
IS  - 1
SP  - 187
EP  - 198
DO  - 10.2298/GENSR1601187V
ER  - 
@article{
author = "Vasić, Tanja and Terzić, Dragan and Milenković, Jasmina and Marković, Jordan and Krnjaja, Vesna and Jevremović, Darko and Stanković, Slavica",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/650",
abstract = "A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover",
volume = "48",
number = "1",
pages = "187-198",
doi = "10.2298/GENSR1601187V"
}
Vasić, T., Terzić, D., Milenković, J., Marković, J., Krnjaja, V., Jevremović, D.,& Stanković, S. (2016). Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover.
Genetika
Društvo genetičara Srbije, Beograd., 48(1), 187-198.
https://doi.org/10.2298/GENSR1601187V
Vasić T, Terzić D, Milenković J, Marković J, Krnjaja V, Jevremović D, Stanković S. Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. Genetika. 2016;48(1):187-198
Vasić Tanja, Terzić Dragan, Milenković Jasmina, Marković Jordan, Krnjaja Vesna, Jevremović Darko, Stanković Slavica, "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover" Genetika, 48, no. 1 (2016):187-198,
https://doi.org/10.2298/GENSR1601187V .

Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield

Mandić, Violeta; Bijelić, Zorica; Krnjaja, Vesna; Tomić, Z.; Đorđević, S.; Stanojević, D.; Dragičević, Vesna

(Scibulcom Ltd, Sofia, 2016)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Tomić, Z.
AU  - Đorđević, S.
AU  - Stanojević, D.
AU  - Dragičević, Vesna
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/616
AB  - The aim of this study was to determine the effects of the inoculation of maize seeds with mixtures of bacteria (Azotobacter chroococum, Azotobacter vinelandi, Bacillus megaterium, Bacillus licheniformis) during different phenophases (6-7 leaves, silking and wax ripeness stage) and on the nitrogen dynamics, total number of microorganisms, number of azotobacter and aminoheterotrophs in Chernozem and grain yield of maize hybrid ZP 684 during 2006, 2007 and 2008. Nitrogen amount in soil was significantly higher in 2006, due to favourable meteorological conditions for microbial activity (higher total number of microorganisms and number of azotobacter), than in 2007 and 2008. The minimum amount of nitrogen was in the stage of wax ripeness although the total number of microorganisms, azotobacter and aminoheterotrophs were the largest. Seed inoculation of maize significantly increases the values of all studied parameters.
PB  - Scibulcom Ltd, Sofia
T2  - Journal of Environmental Protection and Ecology
T1  - Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield
VL  - 17
IS  - 3
SP  - 1003
EP  - 1010
ER  - 
@article{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Tomić, Z. and Đorđević, S. and Stanojević, D. and Dragičević, Vesna",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/616",
abstract = "The aim of this study was to determine the effects of the inoculation of maize seeds with mixtures of bacteria (Azotobacter chroococum, Azotobacter vinelandi, Bacillus megaterium, Bacillus licheniformis) during different phenophases (6-7 leaves, silking and wax ripeness stage) and on the nitrogen dynamics, total number of microorganisms, number of azotobacter and aminoheterotrophs in Chernozem and grain yield of maize hybrid ZP 684 during 2006, 2007 and 2008. Nitrogen amount in soil was significantly higher in 2006, due to favourable meteorological conditions for microbial activity (higher total number of microorganisms and number of azotobacter), than in 2007 and 2008. The minimum amount of nitrogen was in the stage of wax ripeness although the total number of microorganisms, azotobacter and aminoheterotrophs were the largest. Seed inoculation of maize significantly increases the values of all studied parameters.",
publisher = "Scibulcom Ltd, Sofia",
journal = "Journal of Environmental Protection and Ecology",
title = "Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield",
volume = "17",
number = "3",
pages = "1003-1010"
}
Mandić, V., Bijelić, Z., Krnjaja, V., Tomić, Z., Đorđević, S., Stanojević, D.,& Dragičević, V. (2016). Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield.
Journal of Environmental Protection and Ecology
Scibulcom Ltd, Sofia., 17(3), 1003-1010.
Mandić V, Bijelić Z, Krnjaja V, Tomić Z, Đorđević S, Stanojević D, Dragičević V. Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield. Journal of Environmental Protection and Ecology. 2016;17(3):1003-1010
Mandić Violeta, Bijelić Zorica, Krnjaja Vesna, Tomić Z., Đorđević S., Stanojević D., Dragičević Vesna, "Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield" Journal of Environmental Protection and Ecology, 17, no. 3 (2016):1003-1010
2

Fusarium infection and deoxynivalenol contamination in winter wheat

Krnjaja, Vesna; Tomić, Z.; Stanković, Slavica; Petrović, T.; Bijelić, Zorica; Mandić, Violeta; Obradović, Ana

(Institut za stočarstvo, Beograd, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Tomić, Z.
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Obradović, Ana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/606
AB  - In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat.
AB  - U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fusarium infection and deoxynivalenol contamination in winter wheat
T1  - Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice
VL  - 31
IS  - 1
SP  - 123
EP  - 131
DO  - 10.2298/BAH1501123K
ER  - 
@article{
author = "Krnjaja, Vesna and Tomić, Z. and Stanković, Slavica and Petrović, T. and Bijelić, Zorica and Mandić, Violeta and Obradović, Ana",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/606",
abstract = "In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat., U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fusarium infection and deoxynivalenol contamination in winter wheat, Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice",
volume = "31",
number = "1",
pages = "123-131",
doi = "10.2298/BAH1501123K"
}
Krnjaja, V., Tomić, Z., Stanković, S., Petrović, T., Bijelić, Z., Mandić, V.,& Obradović, A. (2015). Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 31(1), 123-131.
https://doi.org/10.2298/BAH1501123K
Krnjaja V, Tomić Z, Stanković S, Petrović T, Bijelić Z, Mandić V, Obradović A. Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice. Biotechnology in Animal Husbandry. 2015;31(1):123-131
Krnjaja Vesna, Tomić Z., Stanković Slavica, Petrović T., Bijelić Zorica, Mandić Violeta, Obradović Ana, "Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice" Biotechnology in Animal Husbandry, 31, no. 1 (2015):123-131,
https://doi.org/10.2298/BAH1501123K .
5

Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain

Tančić, Sonja; Stanković, Slavica; Lević, Jelena; Krnjaja, Vesna

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2015)

TY  - JOUR
AU  - Tančić, Sonja
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/607
AB  - A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp.
AB  - Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain
T1  - Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza
VL  - 30
IS  - 2
SP  - 99
EP  - 105
DO  - 10.2298/pif1502099T
ER  - 
@article{
author = "Tančić, Sonja and Stanković, Slavica and Lević, Jelena and Krnjaja, Vesna",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/607",
abstract = "A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp., Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain, Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza",
volume = "30",
number = "2",
pages = "99-105",
doi = "10.2298/pif1502099T"
}
Tančić, S., Stanković, S., Lević, J.,& Krnjaja, V. (2015). Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza.
Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 30(2), 99-105.
https://doi.org/10.2298/pif1502099T
Tančić S, Stanković S, Lević J, Krnjaja V. Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza. Pesticidi i fitomedicina. 2015;30(2):99-105
Tančić Sonja, Stanković Slavica, Lević Jelena, Krnjaja Vesna, "Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza" Pesticidi i fitomedicina, 30, no. 2 (2015):99-105,
https://doi.org/10.2298/pif1502099T .
7

Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat

Krnjaja, Vesna; Mandić, Violeta; Lević, Jelena; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/602
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
VL  - 67
SP  - 251
EP  - 256
DO  - 10.1016/j.cropro.2014.11.001
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/602",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
volume = "67",
pages = "251-256",
doi = "10.1016/j.cropro.2014.11.001"
}
Krnjaja, V., Mandić, V., Lević, J., Stanković, S., Obradović, A., Petrović, T.,& Vasić, T. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat.
Crop Protection
Elsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001
Krnjaja V, Mandić V, Lević J, Stanković S, Obradović A, Petrović T, Vasić T. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. Crop Protection. 2015;67:251-256
Krnjaja Vesna, Mandić Violeta, Lević Jelena, Stanković Slavica, Obradović Ana, Petrović Tanja, Vasić Tanja, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" Crop Protection, 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 .
15
8
13

Presence of deoxynivalenol in winter wheat treated with fungicides

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Mandić, Violeta; Tomić, Z.; Obradović, Ana

(Institut za stočarstvo, Beograd, 2014)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Mandić, Violeta
AU  - Tomić, Z.
AU  - Obradović, Ana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/566
AB  - Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively).
AB  - U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Presence of deoxynivalenol in winter wheat treated with fungicides
T1  - Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima
VL  - 30
IS  - 1
SP  - 167
EP  - 173
DO  - 10.2298/BAH1401167K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Mandić, Violeta and Tomić, Z. and Obradović, Ana",
year = "2014",
url = "http://rik.mrizp.rs/handle/123456789/566",
abstract = "Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively)., U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Presence of deoxynivalenol in winter wheat treated with fungicides, Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima",
volume = "30",
number = "1",
pages = "167-173",
doi = "10.2298/BAH1401167K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Mandić, V., Tomić, Z.,& Obradović, A. (2014). Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 30(1), 167-173.
https://doi.org/10.2298/BAH1401167K
Krnjaja V, Lević J, Stanković S, Petrović T, Mandić V, Tomić Z, Obradović A. Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima. Biotechnology in Animal Husbandry. 2014;30(1):167-173
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Mandić Violeta, Tomić Z., Obradović Ana, "Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima" Biotechnology in Animal Husbandry, 30, no. 1 (2014):167-173,
https://doi.org/10.2298/BAH1401167K .
1

An outbreak of Aspergillus species in response to environmental conditions in Serbia

Lević, Jelena; Gošić-Dondo, Snežana; Ivanović, Dragica; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra; Stepanić, Ana

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Gošić-Dondo, Snežana
AU  - Ivanović, Dragica
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/510
AB  - The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low.
AB  - U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - An outbreak of Aspergillus species in response to environmental conditions in Serbia
T1  - Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji
VL  - 28
IS  - 3
SP  - 167
EP  - 179
DO  - 10.2298/PIF1303167L
ER  - 
@article{
author = "Lević, Jelena and Gošić-Dondo, Snežana and Ivanović, Dragica and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra and Stepanić, Ana",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/510",
abstract = "The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low., U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "An outbreak of Aspergillus species in response to environmental conditions in Serbia, Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji",
volume = "28",
number = "3",
pages = "167-179",
doi = "10.2298/PIF1303167L"
}
Lević, J., Gošić-Dondo, S., Ivanović, D., Stanković, S., Krnjaja, V., Bočarov-Stančić, A.,& Stepanić, A. (2013). Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji.
Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(3), 167-179.
https://doi.org/10.2298/PIF1303167L
Lević J, Gošić-Dondo S, Ivanović D, Stanković S, Krnjaja V, Bočarov-Stančić A, Stepanić A. Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji. Pesticidi i fitomedicina. 2013;28(3):167-179
Lević Jelena, Gošić-Dondo Snežana, Ivanović Dragica, Stanković Slavica, Krnjaja Vesna, Bočarov-Stančić Aleksandra, Stepanić Ana, "Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji" Pesticidi i fitomedicina, 28, no. 3 (2013):167-179,
https://doi.org/10.2298/PIF1303167L .
31

Molds and mycotoxins in freshly harvested maize

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, Tanja; Lukić, Miloš

(Matica srpska, Novi Sad, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, Tanja
AU  - Lukić, Miloš
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/516
AB  - Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02).
AB  - Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Molds and mycotoxins in freshly harvested maize
T1  - Plesni i mikotoksini u zrnu kukuruza posle berbe
IS  - 124
SP  - 111
EP  - 119
DO  - 10.2298/ZMSPN1324111K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, Tanja and Lukić, Miloš",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/516",
abstract = "Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02)., Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Molds and mycotoxins in freshly harvested maize, Plesni i mikotoksini u zrnu kukuruza posle berbe",
number = "124",
pages = "111-119",
doi = "10.2298/ZMSPN1324111K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T.,& Lukić, M. (2013). Plesni i mikotoksini u zrnu kukuruza posle berbe.
Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(124), 111-119.
https://doi.org/10.2298/ZMSPN1324111K
Krnjaja V, Lević J, Stanković S, Petrović T, Lukić M. Plesni i mikotoksini u zrnu kukuruza posle berbe. Zbornik Matice srpske za prirodne nauke. 2013;(124):111-119
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović Tanja, Lukić Miloš, "Plesni i mikotoksini u zrnu kukuruza posle berbe" Zbornik Matice srpske za prirodne nauke, no. 124 (2013):111-119,
https://doi.org/10.2298/ZMSPN1324111K .
2

The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Vasić, Tanja

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Vasić, Tanja
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/509
AB  - Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail.
AB  - Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi
T1  - Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva
VL  - 28
IS  - 3
SP  - 157
EP  - 165
DO  - 10.2298/PIF1303157K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Vasić, Tanja",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/509",
abstract = "Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail., Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi, Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva",
volume = "28",
number = "3",
pages = "157-165",
doi = "10.2298/PIF1303157K"
}
Krnjaja, V., Lević, J., Stanković, S.,& Vasić, T. (2013). Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva.
Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(3), 157-165.
https://doi.org/10.2298/PIF1303157K
Krnjaja V, Lević J, Stanković S, Vasić T. Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva. Pesticidi i fitomedicina. 2013;28(3):157-165
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Vasić Tanja, "Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva" Pesticidi i fitomedicina, 28, no. 3 (2013):157-165,
https://doi.org/10.2298/PIF1303157K .
3

Moulds and mycotoxins in stored maize grains

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Tomić, Z.; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Tomić, Z.
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/485
AB  - In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58).
AB  - U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Moulds and mycotoxins in stored maize grains
T1  - Plesni i mikotoksini u uskladištenom kukuruzu
VL  - 29
IS  - 3
SP  - 527
EP  - 536
DO  - 10.2298/BAH1303527K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Tomić, Z. and Mandić, Violeta and Bijelić, Zorica",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/485",
abstract = "In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58)., U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Moulds and mycotoxins in stored maize grains, Plesni i mikotoksini u uskladištenom kukuruzu",
volume = "29",
number = "3",
pages = "527-536",
doi = "10.2298/BAH1303527K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Tomić, Z., Mandić, V.,& Bijelić, Z. (2013). Plesni i mikotoksini u uskladištenom kukuruzu.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 29(3), 527-536.
https://doi.org/10.2298/BAH1303527K
Krnjaja V, Lević J, Stanković S, Petrović T, Tomić Z, Mandić V, Bijelić Z. Plesni i mikotoksini u uskladištenom kukuruzu. Biotechnology in Animal Husbandry. 2013;29(3):527-536
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Tomić Z., Mandić Violeta, Bijelić Zorica, "Plesni i mikotoksini u uskladištenom kukuruzu" Biotechnology in Animal Husbandry, 29, no. 3 (2013):527-536,
https://doi.org/10.2298/BAH1303527K .
9