Lević, Jelena

Link to this page

Authority KeyName Variants
6e8b39db-53bb-45e9-9d5f-dad8a41f9517
  • Lević, Jelena (52)
Projects
Reduction of toxigenic Fusarium species and their mycotoxins in production of safe cereal-based foods Proučavanje i čuvanje core kolekcije fitopatogenih gljiva značajnih u poljoprivredi Srbije
Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness Implementation of new biotechnological solution in breeding of cattle, sheep and goats for the purpose of obtaining biologically valuable and safe food
Improvment of maize and soybean traits by molecular and conventional breeding Academy of Finland - 126917
Academy of Finland - 131957 Academy of Finland - 250904
Academy of Finland - 252162 Academy of Finland - 266984
Academy of Finland - 267188 CIMO travel grant
Danish Directorate for Food, Fisheries and Agri Business - FFS05-3 Direction Generale de l'Agriculture, Direction de la Recherche - D31-1162
Direction Generale de l'Agriculture, Direction de la Recherche - D31-3159 Direction Generale de l'Agriculture, Direction de la Recherche - D31-7055
Federal Ministry of Education and Research (BMBF) (GABI-KANADA), Bonn - FKZ 0313711A Felix Thornley Cobbold Trust
Finnish Ministry of Agriculture and Forestry German Academic Exchange Service (DAAD), Bonn - A/06/92183
Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade New products based on cereals and pseudocereals from organic production
Uticaj citoplazmatičke muške sterilnosti i ksenija na prinos zrna i agronomske osobine kukuruza Sustainable conventional and revitalized traditional production of poultry meat and eggs with added value
Improvement of genetic potential and technologies in forage crops production in function of sustainable animal husbandry development Implementation of various rearing - selection and biotechnological methods in breeding of pigs
John Oldacre Foundation Ministere de l'Agriculture, de la Viticulture et de la Protection des Consommateurs-Administration d
Ministry of Agriculture and Food, Norway Ministry of Agriculture of the Czech Republic - 800415

Author's Bibliography

A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes

Pasquali, Matias; Beyer, Marco; Hoffmann, Lucien; Pallez-Barthel, Marine; Piec, Jonathan; Logrieco, Antonio; Moretti, Antonio; Audenaert, Kris; Vanheule, Adriaan; Balmas, Virgilio; Migheli, Quirico; Scherm, Barbara; Basler, Ryan; Boutigny, Anne-Laure; Chrpova, Jana; Czembor, Elzbieta; Gagkaeva, Tatiana; Gonzalez-Jaen, Maria; Marin, Patricia; Hofgaard, Ingerd S.; Koycu, Nagehan D.; Lević, Jelena; Stanković, Slavica; Miedaner, Thomas; Mueller, Marina E. H.; Munaut, Francoise; Scauflaire, Jonathan; Parikka, Paivi; Thrane, Ulf; Uhlig, Silvio; Yli-Mattila, Tapani; Vogelgsang, Susanne

(Frontiers Media Sa, Lausanne, 2016)

TY  - JOUR
AU  - Pasquali, Matias
AU  - Beyer, Marco
AU  - Hoffmann, Lucien
AU  - Pallez-Barthel, Marine
AU  - Piec, Jonathan
AU  - Logrieco, Antonio
AU  - Moretti, Antonio
AU  - Audenaert, Kris
AU  - Vanheule, Adriaan
AU  - Balmas, Virgilio
AU  - Migheli, Quirico
AU  - Scherm, Barbara
AU  - Basler, Ryan
AU  - Boutigny, Anne-Laure
AU  - Chrpova, Jana
AU  - Czembor, Elzbieta
AU  - Gagkaeva, Tatiana
AU  - Gonzalez-Jaen, Maria
AU  - Marin, Patricia
AU  - Hofgaard, Ingerd S.
AU  - Koycu, Nagehan D.
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Miedaner, Thomas
AU  - Mueller, Marina E. H.
AU  - Munaut, Francoise
AU  - Scauflaire, Jonathan
AU  - Parikka, Paivi
AU  - Thrane, Ulf
AU  - Uhlig, Silvio
AU  - Yli-Mattila, Tapani
AU  - Vogelgsang, Susanne
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/655
AB  - Fusarium species, particularly Fusarium graminearum and F culmorum, are the main cause of trichothecene type B contamination in cereals. Data on the distribution of Fusarium trichothecene genotypes in cereals in Europe are scattered in time and space. Furthermore, a common core set of related variables (sampling method, host cultivar, previous crop, etc.) that would allow more effective analysis of factors influencing the spatial and temporal population distribution, is lacking. Consequently, based on the available data, it is difficult to identify factors influencing chemotype distribution and spread at the European level. Here we describe the results of a collaborative integrated work which aims (1) to characterize the trichothecene genotypes of strains from three Fusarium species, collected over the period 2000-2013 and (2) to enhance the standardization of epidemiological data collection. Information on host plant, country of origin, sampling location, year of sampling and previous crop of 1147 F graminearurn, 479 F culmorum, and 3 F cortaderiae strains obtained from 17 European countries was compiled and a map of trichothecene type B genotype distribution was plotted for each species. All information on the strains was collected in a freely accessible and updatable database (www.catalogueeu.luxmcc.lu), which will serve as a starting point for epidemiological analysis of potential spatial and temporal trichothecene genotype shifts in Europe. The analysis of the currently available European dataset showed that in F. grarninearum, the predominant genotype was 15-acetyldeoxynivalenol (15-ADON) (82.9%), followed by 3-acetyldeoxynivalenol (3-ADON) (13.6%), and nivalenol (NIV) (3.5%). In F culmorum, the prevalent genotype was 3-ADON (59.9%), while the NIV genotype accounted for the remaining 40.1%. Both, geographical and temporal patterns of trichothecene genotypes distribution were identified.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes
VL  - 7
DO  - 10.3389/fmicb.2016.00406
UR  - conv_925
ER  - 
@article{
author = "Pasquali, Matias and Beyer, Marco and Hoffmann, Lucien and Pallez-Barthel, Marine and Piec, Jonathan and Logrieco, Antonio and Moretti, Antonio and Audenaert, Kris and Vanheule, Adriaan and Balmas, Virgilio and Migheli, Quirico and Scherm, Barbara and Basler, Ryan and Boutigny, Anne-Laure and Chrpova, Jana and Czembor, Elzbieta and Gagkaeva, Tatiana and Gonzalez-Jaen, Maria and Marin, Patricia and Hofgaard, Ingerd S. and Koycu, Nagehan D. and Lević, Jelena and Stanković, Slavica and Miedaner, Thomas and Mueller, Marina E. H. and Munaut, Francoise and Scauflaire, Jonathan and Parikka, Paivi and Thrane, Ulf and Uhlig, Silvio and Yli-Mattila, Tapani and Vogelgsang, Susanne",
year = "2016",
abstract = "Fusarium species, particularly Fusarium graminearum and F culmorum, are the main cause of trichothecene type B contamination in cereals. Data on the distribution of Fusarium trichothecene genotypes in cereals in Europe are scattered in time and space. Furthermore, a common core set of related variables (sampling method, host cultivar, previous crop, etc.) that would allow more effective analysis of factors influencing the spatial and temporal population distribution, is lacking. Consequently, based on the available data, it is difficult to identify factors influencing chemotype distribution and spread at the European level. Here we describe the results of a collaborative integrated work which aims (1) to characterize the trichothecene genotypes of strains from three Fusarium species, collected over the period 2000-2013 and (2) to enhance the standardization of epidemiological data collection. Information on host plant, country of origin, sampling location, year of sampling and previous crop of 1147 F graminearurn, 479 F culmorum, and 3 F cortaderiae strains obtained from 17 European countries was compiled and a map of trichothecene type B genotype distribution was plotted for each species. All information on the strains was collected in a freely accessible and updatable database (www.catalogueeu.luxmcc.lu), which will serve as a starting point for epidemiological analysis of potential spatial and temporal trichothecene genotype shifts in Europe. The analysis of the currently available European dataset showed that in F. grarninearum, the predominant genotype was 15-acetyldeoxynivalenol (15-ADON) (82.9%), followed by 3-acetyldeoxynivalenol (3-ADON) (13.6%), and nivalenol (NIV) (3.5%). In F culmorum, the prevalent genotype was 3-ADON (59.9%), while the NIV genotype accounted for the remaining 40.1%. Both, geographical and temporal patterns of trichothecene genotypes distribution were identified.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes",
volume = "7",
doi = "10.3389/fmicb.2016.00406",
url = "conv_925"
}
Pasquali, M., Beyer, M., Hoffmann, L., Pallez-Barthel, M., Piec, J., Logrieco, A., Moretti, A., Audenaert, K., Vanheule, A., Balmas, V., Migheli, Q., Scherm, B., Basler, R., Boutigny, A., Chrpova, J., Czembor, E., Gagkaeva, T., Gonzalez-Jaen, M., Marin, P., Hofgaard, I. S., Koycu, N. D., Lević, J., Stanković, S., Miedaner, T., Mueller, M. E. H., Munaut, F., Scauflaire, J., Parikka, P., Thrane, U., Uhlig, S., Yli-Mattila, T.,& Vogelgsang, S.. (2016). A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmicb.2016.00406
conv_925
Pasquali M, Beyer M, Hoffmann L, Pallez-Barthel M, Piec J, Logrieco A, Moretti A, Audenaert K, Vanheule A, Balmas V, Migheli Q, Scherm B, Basler R, Boutigny A, Chrpova J, Czembor E, Gagkaeva T, Gonzalez-Jaen M, Marin P, Hofgaard IS, Koycu ND, Lević J, Stanković S, Miedaner T, Mueller MEH, Munaut F, Scauflaire J, Parikka P, Thrane U, Uhlig S, Yli-Mattila T, Vogelgsang S. A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes. in Frontiers in Microbiology. 2016;7.
doi:10.3389/fmicb.2016.00406
conv_925 .
Pasquali, Matias, Beyer, Marco, Hoffmann, Lucien, Pallez-Barthel, Marine, Piec, Jonathan, Logrieco, Antonio, Moretti, Antonio, Audenaert, Kris, Vanheule, Adriaan, Balmas, Virgilio, Migheli, Quirico, Scherm, Barbara, Basler, Ryan, Boutigny, Anne-Laure, Chrpova, Jana, Czembor, Elzbieta, Gagkaeva, Tatiana, Gonzalez-Jaen, Maria, Marin, Patricia, Hofgaard, Ingerd S., Koycu, Nagehan D., Lević, Jelena, Stanković, Slavica, Miedaner, Thomas, Mueller, Marina E. H., Munaut, Francoise, Scauflaire, Jonathan, Parikka, Paivi, Thrane, Ulf, Uhlig, Silvio, Yli-Mattila, Tapani, Vogelgsang, Susanne, "A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes" in Frontiers in Microbiology, 7 (2016),
https://doi.org/10.3389/fmicb.2016.00406 .,
conv_925 .
4
82
76
84

Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain

Tančić, Sonja; Stanković, Slavica; Lević, Jelena; Krnjaja, Vesna

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2015)

TY  - JOUR
AU  - Tančić, Sonja
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/607
AB  - A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp.
AB  - Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain
T1  - Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza
VL  - 30
IS  - 2
SP  - 99
EP  - 105
DO  - 10.2298/pif1502099T
UR  - conv_609
ER  - 
@article{
author = "Tančić, Sonja and Stanković, Slavica and Lević, Jelena and Krnjaja, Vesna",
year = "2015",
abstract = "A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp., Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain, Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza",
volume = "30",
number = "2",
pages = "99-105",
doi = "10.2298/pif1502099T",
url = "conv_609"
}
Tančić, S., Stanković, S., Lević, J.,& Krnjaja, V.. (2015). Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 30(2), 99-105.
https://doi.org/10.2298/pif1502099T
conv_609
Tančić S, Stanković S, Lević J, Krnjaja V. Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain. in Pesticidi i fitomedicina. 2015;30(2):99-105.
doi:10.2298/pif1502099T
conv_609 .
Tančić, Sonja, Stanković, Slavica, Lević, Jelena, Krnjaja, Vesna, "Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain" in Pesticidi i fitomedicina, 30, no. 2 (2015):99-105,
https://doi.org/10.2298/pif1502099T .,
conv_609 .
7

Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat

Krnjaja, Vesna; Mandić, Violeta; Lević, Jelena; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/602
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
VL  - 67
SP  - 251
EP  - 256
DO  - 10.1016/j.cropro.2014.11.001
UR  - conv_893
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja",
year = "2015",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
volume = "67",
pages = "251-256",
doi = "10.1016/j.cropro.2014.11.001",
url = "conv_893"
}
Krnjaja, V., Mandić, V., Lević, J., Stanković, S., Obradović, A., Petrović, T.,& Vasić, T.. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. in Crop Protection
Elsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001
conv_893
Krnjaja V, Mandić V, Lević J, Stanković S, Obradović A, Petrović T, Vasić T. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. in Crop Protection. 2015;67:251-256.
doi:10.1016/j.cropro.2014.11.001
conv_893 .
Krnjaja, Vesna, Mandić, Violeta, Lević, Jelena, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" in Crop Protection, 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 .,
conv_893 .
16
13
15

Presence of deoxynivalenol in winter wheat treated with fungicides

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Mandić, Violeta; Tomić, Z.; Obradović, Ana

(Institut za stočarstvo, Beograd, 2014)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Mandić, Violeta
AU  - Tomić, Z.
AU  - Obradović, Ana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/566
AB  - Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively).
AB  - U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Presence of deoxynivalenol in winter wheat treated with fungicides
T1  - Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima
VL  - 30
IS  - 1
SP  - 167
EP  - 173
DO  - 10.2298/BAH1401167K
UR  - conv_591
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Mandić, Violeta and Tomić, Z. and Obradović, Ana",
year = "2014",
abstract = "Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively)., U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Presence of deoxynivalenol in winter wheat treated with fungicides, Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima",
volume = "30",
number = "1",
pages = "167-173",
doi = "10.2298/BAH1401167K",
url = "conv_591"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Mandić, V., Tomić, Z.,& Obradović, A.. (2014). Presence of deoxynivalenol in winter wheat treated with fungicides. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 30(1), 167-173.
https://doi.org/10.2298/BAH1401167K
conv_591
Krnjaja V, Lević J, Stanković S, Petrović T, Mandić V, Tomić Z, Obradović A. Presence of deoxynivalenol in winter wheat treated with fungicides. in Biotechnology in Animal Husbandry. 2014;30(1):167-173.
doi:10.2298/BAH1401167K
conv_591 .
Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica, Petrović, T., Mandić, Violeta, Tomić, Z., Obradović, Ana, "Presence of deoxynivalenol in winter wheat treated with fungicides" in Biotechnology in Animal Husbandry, 30, no. 1 (2014):167-173,
https://doi.org/10.2298/BAH1401167K .,
conv_591 .
1

An outbreak of Aspergillus species in response to environmental conditions in Serbia

Lević, Jelena; Gošić-Dondo, Snežana; Ivanović, Dragica; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra; Stepanić, Ana

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Gošić-Dondo, Snežana
AU  - Ivanović, Dragica
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/510
AB  - The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low.
AB  - U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - An outbreak of Aspergillus species in response to environmental conditions in Serbia
T1  - Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji
VL  - 28
IS  - 3
SP  - 167
EP  - 179
DO  - 10.2298/PIF1303167L
UR  - conv_607
ER  - 
@article{
author = "Lević, Jelena and Gošić-Dondo, Snežana and Ivanović, Dragica and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra and Stepanić, Ana",
year = "2013",
abstract = "The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low., U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "An outbreak of Aspergillus species in response to environmental conditions in Serbia, Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji",
volume = "28",
number = "3",
pages = "167-179",
doi = "10.2298/PIF1303167L",
url = "conv_607"
}
Lević, J., Gošić-Dondo, S., Ivanović, D., Stanković, S., Krnjaja, V., Bočarov-Stančić, A.,& Stepanić, A.. (2013). An outbreak of Aspergillus species in response to environmental conditions in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(3), 167-179.
https://doi.org/10.2298/PIF1303167L
conv_607
Lević J, Gošić-Dondo S, Ivanović D, Stanković S, Krnjaja V, Bočarov-Stančić A, Stepanić A. An outbreak of Aspergillus species in response to environmental conditions in Serbia. in Pesticidi i fitomedicina. 2013;28(3):167-179.
doi:10.2298/PIF1303167L
conv_607 .
Lević, Jelena, Gošić-Dondo, Snežana, Ivanović, Dragica, Stanković, Slavica, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, Stepanić, Ana, "An outbreak of Aspergillus species in response to environmental conditions in Serbia" in Pesticidi i fitomedicina, 28, no. 3 (2013):167-179,
https://doi.org/10.2298/PIF1303167L .,
conv_607 .
34

Detection of Rhizomucor pusillus on sunflower seed

Lević, Jelena; Ivanović, D.; Stanković, Slavica; Milivojević, Marija; Vukadinović, Radmila; Stepanić, Ana

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Ivanović, D.
AU  - Stanković, Slavica
AU  - Milivojević, Marija
AU  - Vukadinović, Radmila
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/478
AB  - The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - Detection of Rhizomucor pusillus on sunflower seed
VL  - 36
IS  - 59
SP  - 59
EP  - 70
DO  - 10.2298/HEL1359059L
UR  - conv_513
ER  - 
@article{
author = "Lević, Jelena and Ivanović, D. and Stanković, Slavica and Milivojević, Marija and Vukadinović, Radmila and Stepanić, Ana",
year = "2013",
abstract = "The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Detection of Rhizomucor pusillus on sunflower seed",
volume = "36",
number = "59",
pages = "59-70",
doi = "10.2298/HEL1359059L",
url = "conv_513"
}
Lević, J., Ivanović, D., Stanković, S., Milivojević, M., Vukadinović, R.,& Stepanić, A.. (2013). Detection of Rhizomucor pusillus on sunflower seed. in Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 36(59), 59-70.
https://doi.org/10.2298/HEL1359059L
conv_513
Lević J, Ivanović D, Stanković S, Milivojević M, Vukadinović R, Stepanić A. Detection of Rhizomucor pusillus on sunflower seed. in Helia. 2013;36(59):59-70.
doi:10.2298/HEL1359059L
conv_513 .
Lević, Jelena, Ivanović, D., Stanković, Slavica, Milivojević, Marija, Vukadinović, Radmila, Stepanić, Ana, "Detection of Rhizomucor pusillus on sunflower seed" in Helia, 36, no. 59 (2013):59-70,
https://doi.org/10.2298/HEL1359059L .,
conv_513 .

Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia

Kovačević, Tamara; Lević, Jelena; Stanković, Slavica; Vukojević, Jelena

(Društvo genetičara Srbije, Beograd, 2013)

TY  - JOUR
AU  - Kovačević, Tamara
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Vukojević, Jelena
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/513
AB  - The status of fertility and distribution of mating populations in the G. fujikuroi species complex, isolating from maize, sorghum and wheat cultivated under various agroecological conditions of Serbia, have been studied. A total of 79 field isolates of Fusarium spp. in the section Liseola, which had been reciprocally crossed to standard testers (MAT-1 and MAT-2) from each of the four mating populations of the G. fujikuroi species complex, were selected for these studies. Twenty of 79 isolates belonged to the mating population A (G. moniliformis), 22 to the population D (G. intermedia), 17 to the population E (G. subglutinans) and 20 to the population F (G. thapsina). A mating type MAT-1 was dominant in the populations A (14 MATA-1 :6 MATA-2), D (13 MATA-1 :9 MATA-2) and E (10 MATA-1 :7 MATA-2), while MAT-2 prevailed in the population F (6 MATA-1 : 14 MATA-2). The obtained results indicate that the possibility of sexual reproduction of Fusarium spp., belonging to the A, D, E and F mating populations, is not so frequent phenomenon in Serbia as in other regions world-wide. Consequently, these species will be asexually reproduced under field conditions, particularly species belonging to the F population. These are the first results on the characterisation of three (A, E and F) out of four populations of the G. fujikuroi species complex present in Serbia.
AB  - U radu su proučavani fertilnost i distribucija polnih populacija i tipova u okviru kompleksne vrste G. fujikuroi, poreklom iz različitih biljnih vrsta (kukuruza, gajenog sirka i pšenice), koje su gajene u različitim godinama i agroekološkim uslovima Srbije. Za proučavanja su odabrana 79 poljskih izolata Fusarium spp. iz sekcije Liseola, koji su recipročno ukršteni sa standardnim testerima (MAT-1 i MAT-2) od četiri polne populacije kompleksne vrste G. fujikuroi. U ukrštanjima je fertilno potomstvo dalo 77 od 79 izolata. Od 77 izolata 20 je pripadalo polnoj populaciji A (G. moniliformis), 22 populaciji D (G. intermedia), 15 populaciji E (G. subglutinans) i 20 populaciji F (G. thapsina). Polni tip MAT-1 je dominirao u populacijama A (14 MATA-1 :6 MATA-2), D (13 MATA-1 :9 MATA-2) iE(10 MATA-1 :7 MATA-2), dok je MAT-2 dominirao u populaciji F (6 MATA-1 : 14 MATA-2). Proučavani izolati su bili fertilni kao očevi a sterilni kao majke, izuzev po jedan izolat u populacijama A, D i E koji su bili hermafroditi. Na osnovu polnog tipa (Ne(mt)) efektivni broj za A i F populacije bio je 84.00%, za D je 96.69% i E je 96.89%. Na osnovu učestalosti hermafrodita efektivan broj (Ne(f)) za populaciju A bio je 18.15%, populaciju D 16.60%, populaciju E 20.84% i populaciju F 0.00% od stvarnog broja. Dobijeni rezultati ukazuju da je mogućnost seksualne reprodukcije Fusarium spp., koje pripadaju utvrđenim polnim populacija, nije učestala pojava u Srbiji, kao što je za njih utvrđeno u drugim regionima sveta. Kao posledica toga, ove vrste će se aseksualno reprodukovati u poljskim uslovima, posebno vrsta koja pripada F populaciji. Ovo su prvi rezultati o karakterizaciji tri (A, E i F) od četiri populacije kompleksne vrste G. fujikuroi, koje su prisutne u Srbiji.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia
T1  - Polne populacije kompleksne vrste Gibberella fujikuroi (Sawada) S. Ito poreklom iz kukuruza, gajenog sirka i pšenice u Srbiji
VL  - 45
IS  - 3
SP  - 749
EP  - 760
DO  - 10.2298/GENSR1303749K
UR  - conv_485
ER  - 
@article{
author = "Kovačević, Tamara and Lević, Jelena and Stanković, Slavica and Vukojević, Jelena",
year = "2013",
abstract = "The status of fertility and distribution of mating populations in the G. fujikuroi species complex, isolating from maize, sorghum and wheat cultivated under various agroecological conditions of Serbia, have been studied. A total of 79 field isolates of Fusarium spp. in the section Liseola, which had been reciprocally crossed to standard testers (MAT-1 and MAT-2) from each of the four mating populations of the G. fujikuroi species complex, were selected for these studies. Twenty of 79 isolates belonged to the mating population A (G. moniliformis), 22 to the population D (G. intermedia), 17 to the population E (G. subglutinans) and 20 to the population F (G. thapsina). A mating type MAT-1 was dominant in the populations A (14 MATA-1 :6 MATA-2), D (13 MATA-1 :9 MATA-2) and E (10 MATA-1 :7 MATA-2), while MAT-2 prevailed in the population F (6 MATA-1 : 14 MATA-2). The obtained results indicate that the possibility of sexual reproduction of Fusarium spp., belonging to the A, D, E and F mating populations, is not so frequent phenomenon in Serbia as in other regions world-wide. Consequently, these species will be asexually reproduced under field conditions, particularly species belonging to the F population. These are the first results on the characterisation of three (A, E and F) out of four populations of the G. fujikuroi species complex present in Serbia., U radu su proučavani fertilnost i distribucija polnih populacija i tipova u okviru kompleksne vrste G. fujikuroi, poreklom iz različitih biljnih vrsta (kukuruza, gajenog sirka i pšenice), koje su gajene u različitim godinama i agroekološkim uslovima Srbije. Za proučavanja su odabrana 79 poljskih izolata Fusarium spp. iz sekcije Liseola, koji su recipročno ukršteni sa standardnim testerima (MAT-1 i MAT-2) od četiri polne populacije kompleksne vrste G. fujikuroi. U ukrštanjima je fertilno potomstvo dalo 77 od 79 izolata. Od 77 izolata 20 je pripadalo polnoj populaciji A (G. moniliformis), 22 populaciji D (G. intermedia), 15 populaciji E (G. subglutinans) i 20 populaciji F (G. thapsina). Polni tip MAT-1 je dominirao u populacijama A (14 MATA-1 :6 MATA-2), D (13 MATA-1 :9 MATA-2) iE(10 MATA-1 :7 MATA-2), dok je MAT-2 dominirao u populaciji F (6 MATA-1 : 14 MATA-2). Proučavani izolati su bili fertilni kao očevi a sterilni kao majke, izuzev po jedan izolat u populacijama A, D i E koji su bili hermafroditi. Na osnovu polnog tipa (Ne(mt)) efektivni broj za A i F populacije bio je 84.00%, za D je 96.69% i E je 96.89%. Na osnovu učestalosti hermafrodita efektivan broj (Ne(f)) za populaciju A bio je 18.15%, populaciju D 16.60%, populaciju E 20.84% i populaciju F 0.00% od stvarnog broja. Dobijeni rezultati ukazuju da je mogućnost seksualne reprodukcije Fusarium spp., koje pripadaju utvrđenim polnim populacija, nije učestala pojava u Srbiji, kao što je za njih utvrđeno u drugim regionima sveta. Kao posledica toga, ove vrste će se aseksualno reprodukovati u poljskim uslovima, posebno vrsta koja pripada F populaciji. Ovo su prvi rezultati o karakterizaciji tri (A, E i F) od četiri populacije kompleksne vrste G. fujikuroi, koje su prisutne u Srbiji.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia, Polne populacije kompleksne vrste Gibberella fujikuroi (Sawada) S. Ito poreklom iz kukuruza, gajenog sirka i pšenice u Srbiji",
volume = "45",
number = "3",
pages = "749-760",
doi = "10.2298/GENSR1303749K",
url = "conv_485"
}
Kovačević, T., Lević, J., Stanković, S.,& Vukojević, J.. (2013). Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 45(3), 749-760.
https://doi.org/10.2298/GENSR1303749K
conv_485
Kovačević T, Lević J, Stanković S, Vukojević J. Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia. in Genetika. 2013;45(3):749-760.
doi:10.2298/GENSR1303749K
conv_485 .
Kovačević, Tamara, Lević, Jelena, Stanković, Slavica, Vukojević, Jelena, "Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia" in Genetika, 45, no. 3 (2013):749-760,
https://doi.org/10.2298/GENSR1303749K .,
conv_485 .
3
1
1

Molds and mycotoxins in freshly harvested maize

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, Tanja; Lukić, Miloš

(Matica srpska, Novi Sad, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, Tanja
AU  - Lukić, Miloš
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/516
AB  - Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02).
AB  - Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Molds and mycotoxins in freshly harvested maize
T1  - Plesni i mikotoksini u zrnu kukuruza posle berbe
IS  - 124
SP  - 111
EP  - 119
DO  - 10.2298/ZMSPN1324111K
UR  - conv_60
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, Tanja and Lukić, Miloš",
year = "2013",
abstract = "Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02)., Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Molds and mycotoxins in freshly harvested maize, Plesni i mikotoksini u zrnu kukuruza posle berbe",
number = "124",
pages = "111-119",
doi = "10.2298/ZMSPN1324111K",
url = "conv_60"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T.,& Lukić, M.. (2013). Molds and mycotoxins in freshly harvested maize. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(124), 111-119.
https://doi.org/10.2298/ZMSPN1324111K
conv_60
Krnjaja V, Lević J, Stanković S, Petrović T, Lukić M. Molds and mycotoxins in freshly harvested maize. in Zbornik Matice srpske za prirodne nauke. 2013;(124):111-119.
doi:10.2298/ZMSPN1324111K
conv_60 .
Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica, Petrović, Tanja, Lukić, Miloš, "Molds and mycotoxins in freshly harvested maize" in Zbornik Matice srpske za prirodne nauke, no. 124 (2013):111-119,
https://doi.org/10.2298/ZMSPN1324111K .,
conv_60 .
2

Moulds and mycotoxins in stored maize grains

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Tomić, Z.; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Tomić, Z.
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/485
AB  - In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58).
AB  - U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Moulds and mycotoxins in stored maize grains
T1  - Plesni i mikotoksini u uskladištenom kukuruzu
VL  - 29
IS  - 3
SP  - 527
EP  - 536
DO  - 10.2298/BAH1303527K
UR  - conv_588
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Tomić, Z. and Mandić, Violeta and Bijelić, Zorica",
year = "2013",
abstract = "In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58)., U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Moulds and mycotoxins in stored maize grains, Plesni i mikotoksini u uskladištenom kukuruzu",
volume = "29",
number = "3",
pages = "527-536",
doi = "10.2298/BAH1303527K",
url = "conv_588"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Tomić, Z., Mandić, V.,& Bijelić, Z.. (2013). Moulds and mycotoxins in stored maize grains. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 29(3), 527-536.
https://doi.org/10.2298/BAH1303527K
conv_588
Krnjaja V, Lević J, Stanković S, Petrović T, Tomić Z, Mandić V, Bijelić Z. Moulds and mycotoxins in stored maize grains. in Biotechnology in Animal Husbandry. 2013;29(3):527-536.
doi:10.2298/BAH1303527K
conv_588 .
Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica, Petrović, T., Tomić, Z., Mandić, Violeta, Bijelić, Zorica, "Moulds and mycotoxins in stored maize grains" in Biotechnology in Animal Husbandry, 29, no. 3 (2013):527-536,
https://doi.org/10.2298/BAH1303527K .,
conv_588 .
12

The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Vasić, Tanja

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Vasić, Tanja
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/509
AB  - Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail.
AB  - Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi
T1  - Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva
VL  - 28
IS  - 3
SP  - 157
EP  - 165
DO  - 10.2298/PIF1303157K
UR  - conv_606
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Vasić, Tanja",
year = "2013",
abstract = "Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail., Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi, Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva",
volume = "28",
number = "3",
pages = "157-165",
doi = "10.2298/PIF1303157K",
url = "conv_606"
}
Krnjaja, V., Lević, J., Stanković, S.,& Vasić, T.. (2013). The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(3), 157-165.
https://doi.org/10.2298/PIF1303157K
conv_606
Krnjaja V, Lević J, Stanković S, Vasić T. The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi. in Pesticidi i fitomedicina. 2013;28(3):157-165.
doi:10.2298/PIF1303157K
conv_606 .
Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica, Vasić, Tanja, "The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi" in Pesticidi i fitomedicina, 28, no. 3 (2013):157-165,
https://doi.org/10.2298/PIF1303157K .,
conv_606 .
3

The incidence of Pyrenochaeta terrestris in root of different plant species in Serbia

Lević, Jelena; Petrović, Tijana; Stanković, Slavica; Ivanović, Dragica

(Matica srpska, Novi Sad, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Petrović, Tijana
AU  - Stanković, Slavica
AU  - Ivanović, Dragica
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/502
AB  - Root samples of cereals (oats, wheat, barley, maize and sorghum), vegetables (garlic, onion, pepper, cucumber, pumpkin, carrot and tomato), industrial plant (soya bean) and weeds (Johnson grass, barnyard grass and green bristle-grass) collected in different agroecological conditions in Serbia were analysed for the presence of Pyrenochaeta terrestris. The fungus was found in 42 out of 51 samples (82.4%), while the incidence varied from 2.5 to 72.5%. The highest incidence was detected in cereals (average 30.3%), and then in weeds of the Poaceae family (average 14.2%). Considering single species, maize (up to 72.5% in root) and Johnson grass (up to 37.5%) were mostly attacked by this fungus. The lowest incidence of the fungus was determined in vegetable crops (average 6.7%). Red to reddish discoloration of root was correlated with the incidence of the fungus. Obtained data indicate that P. terrestris is widespread in Serbia and conditions for its development are favourable.
AB  - Uzorci korena žita (ovas, pšenica, ječam, kukuruz i pitomi sirak), povrća (beli luk, crni luk, paprika, krastavac, bundeva, šargarepa i paradajz), industrijskog bilja (soja) i korova (divlji sirak, korovski proso i zeleni muhar), koji su prikupljeni u različitim agroekološkim uslovima u Srbiji, analizirani su na prisustvo Pyrenochaeta terrestris. Gljiva je utvrđena u 42 od 51 uzorka (82,4%), a stepen napada je varirao od 2,5% do 72,5%. Generalno, najveći stepen napada gljive utvrđen je na korenu žita (prosek 30,3%), a zatim na korenu korova (prosek 14,2%) iz porodice Poaceae. Među pojedinačnim vrstama, gljiva je u najvećem stepenu utvrđena na korenu kukuruza (do 72,5%) i divljeg sirka (do 37,5%). Nasuprot tome, gljiva je ređe utvrđena na korenu povrtarskih kultura (prosek 6,7%). Crvenilo (ružičasta, crvenkasta, crvena i ljubičasta boja) korena bilo je u korelaciji sa učestalošću pojave gljive. Dobijeni podaci ukazuju na to da je P. terrestris široko rasprostranjena u Srbiji i da su povoljni uslovi za njen razvoj.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - The incidence of Pyrenochaeta terrestris in root of different plant species in Serbia
T1  - Učestalost pojave Pyrenochaeta terrestris na korenu različitih biljnih vrsta u Srbiji
IS  - 125
SP  - 21
EP  - 30
DO  - 10.2298/ZMSPN1325021L
UR  - conv_62
ER  - 
@article{
author = "Lević, Jelena and Petrović, Tijana and Stanković, Slavica and Ivanović, Dragica",
year = "2013",
abstract = "Root samples of cereals (oats, wheat, barley, maize and sorghum), vegetables (garlic, onion, pepper, cucumber, pumpkin, carrot and tomato), industrial plant (soya bean) and weeds (Johnson grass, barnyard grass and green bristle-grass) collected in different agroecological conditions in Serbia were analysed for the presence of Pyrenochaeta terrestris. The fungus was found in 42 out of 51 samples (82.4%), while the incidence varied from 2.5 to 72.5%. The highest incidence was detected in cereals (average 30.3%), and then in weeds of the Poaceae family (average 14.2%). Considering single species, maize (up to 72.5% in root) and Johnson grass (up to 37.5%) were mostly attacked by this fungus. The lowest incidence of the fungus was determined in vegetable crops (average 6.7%). Red to reddish discoloration of root was correlated with the incidence of the fungus. Obtained data indicate that P. terrestris is widespread in Serbia and conditions for its development are favourable., Uzorci korena žita (ovas, pšenica, ječam, kukuruz i pitomi sirak), povrća (beli luk, crni luk, paprika, krastavac, bundeva, šargarepa i paradajz), industrijskog bilja (soja) i korova (divlji sirak, korovski proso i zeleni muhar), koji su prikupljeni u različitim agroekološkim uslovima u Srbiji, analizirani su na prisustvo Pyrenochaeta terrestris. Gljiva je utvrđena u 42 od 51 uzorka (82,4%), a stepen napada je varirao od 2,5% do 72,5%. Generalno, najveći stepen napada gljive utvrđen je na korenu žita (prosek 30,3%), a zatim na korenu korova (prosek 14,2%) iz porodice Poaceae. Među pojedinačnim vrstama, gljiva je u najvećem stepenu utvrđena na korenu kukuruza (do 72,5%) i divljeg sirka (do 37,5%). Nasuprot tome, gljiva je ređe utvrđena na korenu povrtarskih kultura (prosek 6,7%). Crvenilo (ružičasta, crvenkasta, crvena i ljubičasta boja) korena bilo je u korelaciji sa učestalošću pojave gljive. Dobijeni podaci ukazuju na to da je P. terrestris široko rasprostranjena u Srbiji i da su povoljni uslovi za njen razvoj.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "The incidence of Pyrenochaeta terrestris in root of different plant species in Serbia, Učestalost pojave Pyrenochaeta terrestris na korenu različitih biljnih vrsta u Srbiji",
number = "125",
pages = "21-30",
doi = "10.2298/ZMSPN1325021L",
url = "conv_62"
}
Lević, J., Petrović, T., Stanković, S.,& Ivanović, D.. (2013). The incidence of Pyrenochaeta terrestris in root of different plant species in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(125), 21-30.
https://doi.org/10.2298/ZMSPN1325021L
conv_62
Lević J, Petrović T, Stanković S, Ivanović D. The incidence of Pyrenochaeta terrestris in root of different plant species in Serbia. in Zbornik Matice srpske za prirodne nauke. 2013;(125):21-30.
doi:10.2298/ZMSPN1325021L
conv_62 .
Lević, Jelena, Petrović, Tijana, Stanković, Slavica, Ivanović, Dragica, "The incidence of Pyrenochaeta terrestris in root of different plant species in Serbia" in Zbornik Matice srpske za prirodne nauke, no. 125 (2013):21-30,
https://doi.org/10.2298/ZMSPN1325021L .,
conv_62 .
4

Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia

Lević, Jelena; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra; Ivanović, Dragica

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
AU  - Ivanović, Dragica
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/454
AB  - A total of 41 species of fungi were isolated from seed samples of barley, maize, soybean, and sunflower collected at different locations in Serbia. The majority of detected species occurred on barley (35 of 41 species or 87.8%) comparing to soybean (17 of 41 species or 41.5%), sunflower (16 of 41 species or 39.0%) and maize (15 of 41 species or 36.9%). Species belonging to genera Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium and Rhizopus were present on seeds of all four plant species. Alternaria species were dominant on soybean, barley and sunflower seeds (85.7%, 84.7% and 76.9%). F. verticillioides and Penicillium spp. were mainly isolated from maize seeds (100 and 92.3% respectively), while other species were isolated up to 38.5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae and F. sporotrichioides were the most common Fusarium species isolated from barley (51.1-93.3%), while on the soybean seeds F. oxysporum (71.4%), F. semitectum (57.1%) and F. sporotrichioides (57.1%) were prevalent. Frequency of Fusarium species on sunflower seeds varied from 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) to 15.4% (F. verticillioides). Statistically significant negative correlation (r = -0.678*) was determined for the incidence of F. graminearum and Alternaria spp., as well as, Fusarium spp. and Alternaria spp. (r = -0.614*), on barley seeds. The obtained results revealed that seedborne pathogens were present in most seed samples of important cereals and industrial crops grown under different agroecological conditions in Serbia. Some of the identified fungi are potential producers of mycotoxins, thus their presence is important in terms of reduced food safety for humans and animals. Therefore, an early and accurate diagnosis and pathogen surveillance will provide time for the development and the application of disease strategies.
AB  - Ukupno je izolovana 41 vrsta gljiva iz uzoraka zrna ječma, kukuruza, soje i suncokreta, koji su prikupljeni u različitim lokalitetima u Srbiji. Najveći broj gljiva je utvrđen na zrnu ječma (35 vrsta ili 87,8%) u odnosu na zrno soje (17 vrsta ili 41,5%), suncokreta (16 vrsta ili 39,0%) i kukuruza (15 vrsta ili 36,9%). Vrste rodova Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium i Rhizopus su utvrđene na zrnu sve četiri biljne vrste. Vrste roda Alternaria su dominirale na zrnu ječma (99,5%), suncokreta (52,9%) i soje (50,0%). F. verticillioides (do 92,0%) i Penicillium spp. (do 57,0%) su najčešće izolovane vrste sa zrna kukuruza, dok su ostale vrste izolovane do 38,5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae i F. sporotrichioides su najčešće Fusarium vrste izolovane sa zrna ječma (51,1-93,3%), dok su na zrnu soje dominirale F. oxysporum (71,4%), F. semitectum (57,1%) i F. sporotrichioides (57,1%). Učestalost Fusarium vrsta na zrnu suncokreta je varirala od 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) do 15,4% (F. verticillioides). Na zrnu ječma utvđena je statistički značajna negativna korelacija između intenziteta napada F. graminearum i Alternaria spp. (r = -0,678*), kao i između Fusarium spp. i Alternaria spp. (r = -0,614*). Dobijeni rezultati pokazuju da su patogene gljive učestale i u visokom intenzitetu pojave prisutne na zrnu važnih žita i industrijskog bilja gajenih u različitim agroekološkim uslovima u Srbiji. Neke od identifikovanih gljiva su potencijalni proizvođači mikotoksina i njihovo prisustvo je važno zbog smanjene bezbednosti hrane za ljude i životinje. Stoga, rana i pouzdana identifikacija patogenih gljiva i procena njihovog značaja u proizvodnji kukuruza, strnih žita i industrijskih biljaka, omogućuje da se pravovremeno razvije i primeni strategija za njihovo suzbijanje i ublažavanje šteta.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia
T1  - Pojava i učestalost patogena semena žita i industrijskih biljaka u Srbiji
VL  - 27
IS  - 1
SP  - 33
EP  - 40
DO  - 10.2298/PIF1201033L
UR  - conv_603
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra and Ivanović, Dragica",
year = "2012",
abstract = "A total of 41 species of fungi were isolated from seed samples of barley, maize, soybean, and sunflower collected at different locations in Serbia. The majority of detected species occurred on barley (35 of 41 species or 87.8%) comparing to soybean (17 of 41 species or 41.5%), sunflower (16 of 41 species or 39.0%) and maize (15 of 41 species or 36.9%). Species belonging to genera Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium and Rhizopus were present on seeds of all four plant species. Alternaria species were dominant on soybean, barley and sunflower seeds (85.7%, 84.7% and 76.9%). F. verticillioides and Penicillium spp. were mainly isolated from maize seeds (100 and 92.3% respectively), while other species were isolated up to 38.5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae and F. sporotrichioides were the most common Fusarium species isolated from barley (51.1-93.3%), while on the soybean seeds F. oxysporum (71.4%), F. semitectum (57.1%) and F. sporotrichioides (57.1%) were prevalent. Frequency of Fusarium species on sunflower seeds varied from 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) to 15.4% (F. verticillioides). Statistically significant negative correlation (r = -0.678*) was determined for the incidence of F. graminearum and Alternaria spp., as well as, Fusarium spp. and Alternaria spp. (r = -0.614*), on barley seeds. The obtained results revealed that seedborne pathogens were present in most seed samples of important cereals and industrial crops grown under different agroecological conditions in Serbia. Some of the identified fungi are potential producers of mycotoxins, thus their presence is important in terms of reduced food safety for humans and animals. Therefore, an early and accurate diagnosis and pathogen surveillance will provide time for the development and the application of disease strategies., Ukupno je izolovana 41 vrsta gljiva iz uzoraka zrna ječma, kukuruza, soje i suncokreta, koji su prikupljeni u različitim lokalitetima u Srbiji. Najveći broj gljiva je utvrđen na zrnu ječma (35 vrsta ili 87,8%) u odnosu na zrno soje (17 vrsta ili 41,5%), suncokreta (16 vrsta ili 39,0%) i kukuruza (15 vrsta ili 36,9%). Vrste rodova Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium i Rhizopus su utvrđene na zrnu sve četiri biljne vrste. Vrste roda Alternaria su dominirale na zrnu ječma (99,5%), suncokreta (52,9%) i soje (50,0%). F. verticillioides (do 92,0%) i Penicillium spp. (do 57,0%) su najčešće izolovane vrste sa zrna kukuruza, dok su ostale vrste izolovane do 38,5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae i F. sporotrichioides su najčešće Fusarium vrste izolovane sa zrna ječma (51,1-93,3%), dok su na zrnu soje dominirale F. oxysporum (71,4%), F. semitectum (57,1%) i F. sporotrichioides (57,1%). Učestalost Fusarium vrsta na zrnu suncokreta je varirala od 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) do 15,4% (F. verticillioides). Na zrnu ječma utvđena je statistički značajna negativna korelacija između intenziteta napada F. graminearum i Alternaria spp. (r = -0,678*), kao i između Fusarium spp. i Alternaria spp. (r = -0,614*). Dobijeni rezultati pokazuju da su patogene gljive učestale i u visokom intenzitetu pojave prisutne na zrnu važnih žita i industrijskog bilja gajenih u različitim agroekološkim uslovima u Srbiji. Neke od identifikovanih gljiva su potencijalni proizvođači mikotoksina i njihovo prisustvo je važno zbog smanjene bezbednosti hrane za ljude i životinje. Stoga, rana i pouzdana identifikacija patogenih gljiva i procena njihovog značaja u proizvodnji kukuruza, strnih žita i industrijskih biljaka, omogućuje da se pravovremeno razvije i primeni strategija za njihovo suzbijanje i ublažavanje šteta.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia, Pojava i učestalost patogena semena žita i industrijskih biljaka u Srbiji",
volume = "27",
number = "1",
pages = "33-40",
doi = "10.2298/PIF1201033L",
url = "conv_603"
}
Lević, J., Stanković, S., Krnjaja, V., Bočarov-Stančić, A.,& Ivanović, D.. (2012). Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 27(1), 33-40.
https://doi.org/10.2298/PIF1201033L
conv_603
Lević J, Stanković S, Krnjaja V, Bočarov-Stančić A, Ivanović D. Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia. in Pesticidi i fitomedicina. 2012;27(1):33-40.
doi:10.2298/PIF1201033L
conv_603 .
Lević, Jelena, Stanković, Slavica, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, Ivanović, Dragica, "Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia" in Pesticidi i fitomedicina, 27, no. 1 (2012):33-40,
https://doi.org/10.2298/PIF1201033L .,
conv_603 .
14

Pathogenicity of T. terrestris on maize seedlings

Lević, Jelena; Petrović, Tijana; Stanković, Slavica; Ivanović, Dragica

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Petrović, Tijana
AU  - Stanković, Slavica
AU  - Ivanović, Dragica
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/453
AB  - Pathogenicity of P. terrestris was determined by the Knop's medium slants method in test tubes. Isolates originated from the roots of maize (Zea mays L.), barley (Hordeum vulgare L.), Johnson grass (Sorghum halepense Pers.), sorghum (Sorghum bicolour (L.) Moench.), garlic (Allium sativum L.), onion (Allium cepa L.), barnyard millet (Echinochloa crus-galli (L.) P.Beauv.) and green foxtail (Setaria viridis (L.) P.B.). A fragment of a fungal colony, cultivated on PDA, was placed on the bottom of Knop's medium slant in each test tube and then sterilised a maize seed was placed 2 cm away from the inoculum. After 21-day inoculation of seeds, the intensity of the development of symptoms on maize seedlings was estimated. The reddish or dark pigment on the root, mesocotyl and/or coleoptyl of seedlings was an indicator for the infection by the fungus under in vitro conditions. Based on the pathogenicity test, the isolates were classified into the following three groups: slightly (3 isolates), moderately (6 isolates) and very pathogenic (6 isolates) to maize seedlings. The obtained results show that P. terrestris, originating from different hosts, can be a maize pathogen. These results can explain the high frequency and high incidence of this fungus on maize roots in Serbia.
AB  - Za utvrđivanje patogenosti izolata P. terrestris korišćena je metoda sa zakošenom Knopovom podlogom u test-epruveti. Poreklo izolata je sa korena kukuruza (Zea mays L.), ječma (Hordeum vulgare L.), divljeg sirka (Sorghum halepense Pers.), gajenog sirka (Sorghum bicolour (L.) Moench.), belog luka (Allium sativum L.), crnog luka (Allium cepa L.), korovskog prosa (Echinochloa crus-galli L.) i zelenog muhara (Setaria viridis (L.) P.B.). Fragment kolonije gljive, gajene na PDA, je stavljen na donji deo zakošene Knopove podloge u epruveti i 2 cm iznad toga sterilisano seme kukuruza. Nakon 21 dana od inokulacije semena ocenjen je intenzitet razvoja simptoma na klijanacima kukuruza. Crvenkast ili mrki pigment na korenu, mezokotilu i/ili koleoptilu klijanaca je bio indikator za infekciju gljivom u in vitro uslovima. Na osnovu testa patogenosti izolati gljive su grupisani u sledeće tri kategorije: slabo (3 izolata), srednje (6 izolata) i jako patogeni (6 izolata) za klijance kukuruza. Dobijeni rezultati ukazuju da je P. terrestris, poreklom sa različitih domaćina, patogen za kukuruz. Ovi rezultati mogu objasniti učestalost i intenzitet pojave ove gljive na korenu kukuruza u Srbiji.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Pathogenicity of T. terrestris on maize seedlings
T1  - Patogenost P. terrestris na klijancima kukuruza
VL  - 27
IS  - 3
SP  - 213
EP  - 217
DO  - 10.2298/PIF1203213L
UR  - conv_604
ER  - 
@article{
author = "Lević, Jelena and Petrović, Tijana and Stanković, Slavica and Ivanović, Dragica",
year = "2012",
abstract = "Pathogenicity of P. terrestris was determined by the Knop's medium slants method in test tubes. Isolates originated from the roots of maize (Zea mays L.), barley (Hordeum vulgare L.), Johnson grass (Sorghum halepense Pers.), sorghum (Sorghum bicolour (L.) Moench.), garlic (Allium sativum L.), onion (Allium cepa L.), barnyard millet (Echinochloa crus-galli (L.) P.Beauv.) and green foxtail (Setaria viridis (L.) P.B.). A fragment of a fungal colony, cultivated on PDA, was placed on the bottom of Knop's medium slant in each test tube and then sterilised a maize seed was placed 2 cm away from the inoculum. After 21-day inoculation of seeds, the intensity of the development of symptoms on maize seedlings was estimated. The reddish or dark pigment on the root, mesocotyl and/or coleoptyl of seedlings was an indicator for the infection by the fungus under in vitro conditions. Based on the pathogenicity test, the isolates were classified into the following three groups: slightly (3 isolates), moderately (6 isolates) and very pathogenic (6 isolates) to maize seedlings. The obtained results show that P. terrestris, originating from different hosts, can be a maize pathogen. These results can explain the high frequency and high incidence of this fungus on maize roots in Serbia., Za utvrđivanje patogenosti izolata P. terrestris korišćena je metoda sa zakošenom Knopovom podlogom u test-epruveti. Poreklo izolata je sa korena kukuruza (Zea mays L.), ječma (Hordeum vulgare L.), divljeg sirka (Sorghum halepense Pers.), gajenog sirka (Sorghum bicolour (L.) Moench.), belog luka (Allium sativum L.), crnog luka (Allium cepa L.), korovskog prosa (Echinochloa crus-galli L.) i zelenog muhara (Setaria viridis (L.) P.B.). Fragment kolonije gljive, gajene na PDA, je stavljen na donji deo zakošene Knopove podloge u epruveti i 2 cm iznad toga sterilisano seme kukuruza. Nakon 21 dana od inokulacije semena ocenjen je intenzitet razvoja simptoma na klijanacima kukuruza. Crvenkast ili mrki pigment na korenu, mezokotilu i/ili koleoptilu klijanaca je bio indikator za infekciju gljivom u in vitro uslovima. Na osnovu testa patogenosti izolati gljive su grupisani u sledeće tri kategorije: slabo (3 izolata), srednje (6 izolata) i jako patogeni (6 izolata) za klijance kukuruza. Dobijeni rezultati ukazuju da je P. terrestris, poreklom sa različitih domaćina, patogen za kukuruz. Ovi rezultati mogu objasniti učestalost i intenzitet pojave ove gljive na korenu kukuruza u Srbiji.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Pathogenicity of T. terrestris on maize seedlings, Patogenost P. terrestris na klijancima kukuruza",
volume = "27",
number = "3",
pages = "213-217",
doi = "10.2298/PIF1203213L",
url = "conv_604"
}
Lević, J., Petrović, T., Stanković, S.,& Ivanović, D.. (2012). Pathogenicity of T. terrestris on maize seedlings. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 27(3), 213-217.
https://doi.org/10.2298/PIF1203213L
conv_604
Lević J, Petrović T, Stanković S, Ivanović D. Pathogenicity of T. terrestris on maize seedlings. in Pesticidi i fitomedicina. 2012;27(3):213-217.
doi:10.2298/PIF1203213L
conv_604 .
Lević, Jelena, Petrović, Tijana, Stanković, Slavica, Ivanović, Dragica, "Pathogenicity of T. terrestris on maize seedlings" in Pesticidi i fitomedicina, 27, no. 3 (2012):213-217,
https://doi.org/10.2298/PIF1203213L .,
conv_604 .
1

Distribution of moulds and mycotoxins in maize grain silage in the trench silo

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Stojanović, Lj.; Radović, Čedomir; Gogić, M.

(Institut za stočarstvo, Beograd, 2012)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Stojanović, Lj.
AU  - Radović, Čedomir
AU  - Gogić, M.
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/443
AB  - In this study the mycological and mycotoxicological contamination of maize silage samples collected from the bottom (BL), middle (ML) and top (TL) layer in the silage trench silo was studied. Mycological testing of the silage contamination showed the presence of five potential toxigenic fungi of the genera, Aspergillus, Fusarium, Mucor, Penicillium and Rhizopus with their total number ranging from 1 to 10 x 103 CFU g-1. Also, four mycotoxins were detected as followed, aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1). Mycotoxins AFB1 and FB1 were detected as 100% positive in all the samples tested. An average concentration of AFB1 was 2.31 μg kg-1, 2.51 μg kg-1 and 2.81 μg kg-1 in BL, ML and TL respectively, while the concentration of FB1 was 1292.00 μg kg-1, 1298.00 μg kg-1 and 1902.00 μg kg-1 in BL, TL and ML respectively. Average concentration of toxin ZON in positive samples was 15.69 μg kg-1 in BL and 30.68 μg kg-1 in ML, while its presence in the samples taken from the top layer was not detected. An average concentration of DON toxin in positive samples were in the range of 41.71 μg kg-1 for TL, 79.20 μg kg-1 for ML, while it was 97.00 μg kg-1 for BL. There was no statistically significant correlation between moisture content and total number of moulds and concentrations of certain mycotoxins. However, statistically highly significant correlations between the total number of moulds and AFB1 concentration (r = 1.0), ZON (r = 0.99) and FB1 (r = 0.91) were established, while weak correlation was obtained in the case of DON and total mould counts (r = 0.54).
AB  - U radu je ispitivana mikološka i mikotoksikološka kontaminacija uzoraka silaže od zrna kukuruza sakupljenih iz donjeg (DS), srednjeg (SS) i gornjeg (GS) sloja silaže u silo-trenču. Mikološkim ispitivanjima kontaminacije silaže ustanovljeno je prisustvo pet potencijalno toksigenih rodova plesni, Aspergillus, Fusarium, Mucor, Penicillium i Rhizopus sa ukupnim brojem od 1-10 x 103 CFU g- 1. Takođe, detektovana su i četiri mikotoksina kao što su aflatoksin B1 (AFB1), zearalenon (ZON), deoksinivalenol (DON) i fumonizin B1 (FB1). Mikotoksini AFB1 i FB1 detektovani su kao 100% pozitivni u svim ispitivanim uzorcima. Prosečna koncentracija AFB1 bila je 2,31 μg kg-1 za GS, 2,51 μg kg-1 za DS i 2,81 μg kg-1 za SS, dok je prosečna koncentracija FB1 bila 1292,00 μg kg-1 za DS, 1298,00 μg kg-1 za GS i 1902,00 μg kg-1 za SS. Prosečna koncentracija ZON toksina u pozitivnim uzorcima je 15,69 μg kg-1 za DS i 30,68 μg kg-1 za SS, dok njegovo prisustvo nije detektovano u uzorcima iz gornjeg sloja. Prosečna koncentracija DON toksina u pozitivnim uzorcima bila je od 41,71 μg kg-1 za GS, 79,20 μg kg-1 za SS do 97,00 μg kg-1 za DS. Nije utvrđena statistički značajna korelacija sardžaja vlage sa ukupnim brojem plesni i koncentracijom pojedinih mikotoksina. Međutim, utvrđena je statistički veoma značajna korelacija između ukupnog broja plesni i koncentracije AFB1 (r = 1,0), ZON (r = 0,99) i FB1 (r = 0,91), a nešto manja u slučaju DON i ukupnog broja plesni (r = 0,54).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Distribution of moulds and mycotoxins in maize grain silage in the trench silo
T1  - Distribucija plesni i mikotoksina u silaži od zrna kukuruza u silo-trenču
VL  - 28
IS  - 4
SP  - 845
EP  - 854
DO  - 10.2298/BAH1204845K
UR  - conv_587
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Stojanović, Lj. and Radović, Čedomir and Gogić, M.",
year = "2012",
abstract = "In this study the mycological and mycotoxicological contamination of maize silage samples collected from the bottom (BL), middle (ML) and top (TL) layer in the silage trench silo was studied. Mycological testing of the silage contamination showed the presence of five potential toxigenic fungi of the genera, Aspergillus, Fusarium, Mucor, Penicillium and Rhizopus with their total number ranging from 1 to 10 x 103 CFU g-1. Also, four mycotoxins were detected as followed, aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1). Mycotoxins AFB1 and FB1 were detected as 100% positive in all the samples tested. An average concentration of AFB1 was 2.31 μg kg-1, 2.51 μg kg-1 and 2.81 μg kg-1 in BL, ML and TL respectively, while the concentration of FB1 was 1292.00 μg kg-1, 1298.00 μg kg-1 and 1902.00 μg kg-1 in BL, TL and ML respectively. Average concentration of toxin ZON in positive samples was 15.69 μg kg-1 in BL and 30.68 μg kg-1 in ML, while its presence in the samples taken from the top layer was not detected. An average concentration of DON toxin in positive samples were in the range of 41.71 μg kg-1 for TL, 79.20 μg kg-1 for ML, while it was 97.00 μg kg-1 for BL. There was no statistically significant correlation between moisture content and total number of moulds and concentrations of certain mycotoxins. However, statistically highly significant correlations between the total number of moulds and AFB1 concentration (r = 1.0), ZON (r = 0.99) and FB1 (r = 0.91) were established, while weak correlation was obtained in the case of DON and total mould counts (r = 0.54)., U radu je ispitivana mikološka i mikotoksikološka kontaminacija uzoraka silaže od zrna kukuruza sakupljenih iz donjeg (DS), srednjeg (SS) i gornjeg (GS) sloja silaže u silo-trenču. Mikološkim ispitivanjima kontaminacije silaže ustanovljeno je prisustvo pet potencijalno toksigenih rodova plesni, Aspergillus, Fusarium, Mucor, Penicillium i Rhizopus sa ukupnim brojem od 1-10 x 103 CFU g- 1. Takođe, detektovana su i četiri mikotoksina kao što su aflatoksin B1 (AFB1), zearalenon (ZON), deoksinivalenol (DON) i fumonizin B1 (FB1). Mikotoksini AFB1 i FB1 detektovani su kao 100% pozitivni u svim ispitivanim uzorcima. Prosečna koncentracija AFB1 bila je 2,31 μg kg-1 za GS, 2,51 μg kg-1 za DS i 2,81 μg kg-1 za SS, dok je prosečna koncentracija FB1 bila 1292,00 μg kg-1 za DS, 1298,00 μg kg-1 za GS i 1902,00 μg kg-1 za SS. Prosečna koncentracija ZON toksina u pozitivnim uzorcima je 15,69 μg kg-1 za DS i 30,68 μg kg-1 za SS, dok njegovo prisustvo nije detektovano u uzorcima iz gornjeg sloja. Prosečna koncentracija DON toksina u pozitivnim uzorcima bila je od 41,71 μg kg-1 za GS, 79,20 μg kg-1 za SS do 97,00 μg kg-1 za DS. Nije utvrđena statistički značajna korelacija sardžaja vlage sa ukupnim brojem plesni i koncentracijom pojedinih mikotoksina. Međutim, utvrđena je statistički veoma značajna korelacija između ukupnog broja plesni i koncentracije AFB1 (r = 1,0), ZON (r = 0,99) i FB1 (r = 0,91), a nešto manja u slučaju DON i ukupnog broja plesni (r = 0,54).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Distribution of moulds and mycotoxins in maize grain silage in the trench silo, Distribucija plesni i mikotoksina u silaži od zrna kukuruza u silo-trenču",
volume = "28",
number = "4",
pages = "845-854",
doi = "10.2298/BAH1204845K",
url = "conv_587"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Stojanović, Lj., Radović, Č.,& Gogić, M.. (2012). Distribution of moulds and mycotoxins in maize grain silage in the trench silo. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 28(4), 845-854.
https://doi.org/10.2298/BAH1204845K
conv_587
Krnjaja V, Lević J, Stanković S, Petrović T, Stojanović L, Radović Č, Gogić M. Distribution of moulds and mycotoxins in maize grain silage in the trench silo. in Biotechnology in Animal Husbandry. 2012;28(4):845-854.
doi:10.2298/BAH1204845K
conv_587 .
Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica, Petrović, T., Stojanović, Lj., Radović, Čedomir, Gogić, M., "Distribution of moulds and mycotoxins in maize grain silage in the trench silo" in Biotechnology in Animal Husbandry, 28, no. 4 (2012):845-854,
https://doi.org/10.2298/BAH1204845K .,
conv_587 .
1

Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin

Tančić, Sonja; Stanković, Slavica; Lević, Jelena; Krnjaja, Vesna; Vukojević, Jelena

(Društvo genetičara Srbije, Beograd, 2012)

TY  - JOUR
AU  - Tančić, Sonja
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
AU  - Vukojević, Jelena
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/466
AB  - Species of the genus Fusarium are characterised by the exceptional intraspecies and interspecies variability in respect to morphological, physiological and genetic properties. Intraspecies and interspecies diversity of Fusarium verticillioides and Fusarium proliferatum isolates in the production of fumonisin B1 according to their origin from maize and wheat grains was studied. Fumonisin B1 production potential of investigated 42 isolates was assessed by HPTLC and ELISA method. All 22 and 20 investigated F. verticillioides and F. proliferatum isolates, respectively, had the ability to produce fumonisin B1 toxin. Fumonisin B1 production potential of F. verticillioides and F. proliferatum varied from 88.60 ppm to 1,300.60 ppm and from 2.37 ppm to 1,246.00 ppm, respectively. According to Mann-Whitnev U test, there were no significant differences between the fumonisin B1 production mean values of isolates of F. verticillioides originated from maize and wheat (628.13 ppm and 696.38 ppm, respectively), as well as, fumonisin B1 production mean values of F. proliferatum isolates (212.32 ppm and 158.07 ppm, respectively). Variability coefficient values indicated that fumonisin B1 production potential variability was more expressed in maize than wheat originated isolates for both species.
AB  - Vrste roda Fusarium karakteriše izuzetna intraspecijska i interspecijska varijabilnost u pogledu morfoloških, fizioloških i genetičkih svojstava. Proučavan je intraspecijski i interspecijski diverzitet izolata vrsta Fusarium verticillioides i Fusarium proliferatum u produkciji fumonizina B1 zavisno od njihovog porekla sa zrna kukuruza i pšenice. Potencijal sinteze fumonizina B1 kod 42 izolata je ispitivan HPTLC i ELISA metodom. Svih 22 izolata vrste F. verticillioides i 20 izolata vrste F. proliferatum su imali sposobnost stvaranja toksina fumonizina B1. Potencijal za sintezu fumonizina je varirao od 88.60 ppm do 1,300.60 ppm kod izolata vrste F. verticillioides i od 2.37 ppm do 1,246.00 ppm kod F. proliferatum izolata. Prema Mann-Whitnev U testu nije bilo statistički značajnih razlika srednjih vrednosti sintetisanog FB1 između F. verticillioides izolata poreklom sa kukuruza (628.13 ppm) i pšenice (696.38 ppm), kao ni kod F. proliferatum izolata poreklom sa kukuruza (212.32 ppm) i pšenice (158.07 ppm). Visoke vrednosti koeficijenta varijacije ukazuju da je varijabilnost u potencijalu sinteze fumonizina B1 bila više izražena kod izolata poreklom sa kukuruza nego pšenice kod obe ispitivane vrste.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin
T1  - Diverzitet Fusarium verticillioides i F. proliferatum izolata u potencijalu sinteze fumonizina B1 zavisno od porekla
VL  - 44
IS  - 1
SP  - 163
EP  - 176
DO  - 10.2298/GENSR1201163T
UR  - conv_463
ER  - 
@article{
author = "Tančić, Sonja and Stanković, Slavica and Lević, Jelena and Krnjaja, Vesna and Vukojević, Jelena",
year = "2012",
abstract = "Species of the genus Fusarium are characterised by the exceptional intraspecies and interspecies variability in respect to morphological, physiological and genetic properties. Intraspecies and interspecies diversity of Fusarium verticillioides and Fusarium proliferatum isolates in the production of fumonisin B1 according to their origin from maize and wheat grains was studied. Fumonisin B1 production potential of investigated 42 isolates was assessed by HPTLC and ELISA method. All 22 and 20 investigated F. verticillioides and F. proliferatum isolates, respectively, had the ability to produce fumonisin B1 toxin. Fumonisin B1 production potential of F. verticillioides and F. proliferatum varied from 88.60 ppm to 1,300.60 ppm and from 2.37 ppm to 1,246.00 ppm, respectively. According to Mann-Whitnev U test, there were no significant differences between the fumonisin B1 production mean values of isolates of F. verticillioides originated from maize and wheat (628.13 ppm and 696.38 ppm, respectively), as well as, fumonisin B1 production mean values of F. proliferatum isolates (212.32 ppm and 158.07 ppm, respectively). Variability coefficient values indicated that fumonisin B1 production potential variability was more expressed in maize than wheat originated isolates for both species., Vrste roda Fusarium karakteriše izuzetna intraspecijska i interspecijska varijabilnost u pogledu morfoloških, fizioloških i genetičkih svojstava. Proučavan je intraspecijski i interspecijski diverzitet izolata vrsta Fusarium verticillioides i Fusarium proliferatum u produkciji fumonizina B1 zavisno od njihovog porekla sa zrna kukuruza i pšenice. Potencijal sinteze fumonizina B1 kod 42 izolata je ispitivan HPTLC i ELISA metodom. Svih 22 izolata vrste F. verticillioides i 20 izolata vrste F. proliferatum su imali sposobnost stvaranja toksina fumonizina B1. Potencijal za sintezu fumonizina je varirao od 88.60 ppm do 1,300.60 ppm kod izolata vrste F. verticillioides i od 2.37 ppm do 1,246.00 ppm kod F. proliferatum izolata. Prema Mann-Whitnev U testu nije bilo statistički značajnih razlika srednjih vrednosti sintetisanog FB1 između F. verticillioides izolata poreklom sa kukuruza (628.13 ppm) i pšenice (696.38 ppm), kao ni kod F. proliferatum izolata poreklom sa kukuruza (212.32 ppm) i pšenice (158.07 ppm). Visoke vrednosti koeficijenta varijacije ukazuju da je varijabilnost u potencijalu sinteze fumonizina B1 bila više izražena kod izolata poreklom sa kukuruza nego pšenice kod obe ispitivane vrste.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin, Diverzitet Fusarium verticillioides i F. proliferatum izolata u potencijalu sinteze fumonizina B1 zavisno od porekla",
volume = "44",
number = "1",
pages = "163-176",
doi = "10.2298/GENSR1201163T",
url = "conv_463"
}
Tančić, S., Stanković, S., Lević, J., Krnjaja, V.,& Vukojević, J.. (2012). Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin. in Genetika
Društvo genetičara Srbije, Beograd., 44(1), 163-176.
https://doi.org/10.2298/GENSR1201163T
conv_463
Tančić S, Stanković S, Lević J, Krnjaja V, Vukojević J. Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin. in Genetika. 2012;44(1):163-176.
doi:10.2298/GENSR1201163T
conv_463 .
Tančić, Sonja, Stanković, Slavica, Lević, Jelena, Krnjaja, Vesna, Vukojević, Jelena, "Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin" in Genetika, 44, no. 1 (2012):163-176,
https://doi.org/10.2298/GENSR1201163T .,
conv_463 .
9
9
10

Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia

Župunski, Vesna; Jevtić, Radivoje; Ignjatović-Micić, Dragana; Stanković, Slavica; Lević, Jelena; Ivanović, D.; Nikolić, Ana

(Ista-Int Seed Testing Assoc, Bassersdorf, 2012)

TY  - JOUR
AU  - Župunski, Vesna
AU  - Jevtić, Radivoje
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Ivanović, D.
AU  - Nikolić, Ana
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/414
AB  - With the growing importance of organic production and resurgence of bunt fungi in organic farming conditions, the present study was undertaken to determine incidence and contamination level of Tilletia species in conventionally produced non-processed seed of wheat in Vojvodina. Analysis of 151 samples of basic, certified and commercial seed revealed that 129 samples were contaminated with Tilletia species. The predominant species was T. caries, which was found in 127 samples. However, teliospores of Tilletia species, which have not been registered in Serbia before, were found in five samples. These teliospores had a prominent gelatinous sheath together with conspicuous, deep reticulation and it was assumed that some of them might be of the quarantined T. contraversa, whilst others were identified as T. bromi. Contamination level of basic and certified seed was about one teliospore per 10 seeds, while four out of 16 commercial seed samples were contaminated with more than 900 teliospores per seed which exceeds the allowed contamination level.
PB  - Ista-Int Seed Testing Assoc, Bassersdorf
T2  - Seed Science and Technology
T1  - Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia
VL  - 40
IS  - 3
SP  - 320
EP  - 332
DO  - 10.15258/sst.2012.40.3.04
UR  - conv_831
ER  - 
@article{
author = "Župunski, Vesna and Jevtić, Radivoje and Ignjatović-Micić, Dragana and Stanković, Slavica and Lević, Jelena and Ivanović, D. and Nikolić, Ana",
year = "2012",
abstract = "With the growing importance of organic production and resurgence of bunt fungi in organic farming conditions, the present study was undertaken to determine incidence and contamination level of Tilletia species in conventionally produced non-processed seed of wheat in Vojvodina. Analysis of 151 samples of basic, certified and commercial seed revealed that 129 samples were contaminated with Tilletia species. The predominant species was T. caries, which was found in 127 samples. However, teliospores of Tilletia species, which have not been registered in Serbia before, were found in five samples. These teliospores had a prominent gelatinous sheath together with conspicuous, deep reticulation and it was assumed that some of them might be of the quarantined T. contraversa, whilst others were identified as T. bromi. Contamination level of basic and certified seed was about one teliospore per 10 seeds, while four out of 16 commercial seed samples were contaminated with more than 900 teliospores per seed which exceeds the allowed contamination level.",
publisher = "Ista-Int Seed Testing Assoc, Bassersdorf",
journal = "Seed Science and Technology",
title = "Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia",
volume = "40",
number = "3",
pages = "320-332",
doi = "10.15258/sst.2012.40.3.04",
url = "conv_831"
}
Župunski, V., Jevtić, R., Ignjatović-Micić, D., Stanković, S., Lević, J., Ivanović, D.,& Nikolić, A.. (2012). Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia. in Seed Science and Technology
Ista-Int Seed Testing Assoc, Bassersdorf., 40(3), 320-332.
https://doi.org/10.15258/sst.2012.40.3.04
conv_831
Župunski V, Jevtić R, Ignjatović-Micić D, Stanković S, Lević J, Ivanović D, Nikolić A. Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia. in Seed Science and Technology. 2012;40(3):320-332.
doi:10.15258/sst.2012.40.3.04
conv_831 .
Župunski, Vesna, Jevtić, Radivoje, Ignjatović-Micić, Dragana, Stanković, Slavica, Lević, Jelena, Ivanović, D., Nikolić, Ana, "Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia" in Seed Science and Technology, 40, no. 3 (2012):320-332,
https://doi.org/10.15258/sst.2012.40.3.04 .,
conv_831 .
3
2
1

Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties

Lević, Jelena; Petrović, Tijana; Stanković, Slavica; Stanković, Goran; Krnjaja, Vesna

(Natl Agricultural Research & Development Inst, Fundulea, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Petrović, Tijana
AU  - Stanković, Slavica
AU  - Stanković, Goran
AU  - Krnjaja, Vesna
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/412
AB  - Rot symptoms that Pyrenochaeta terrestris causes on the root of each internode of maize hybrids belonging to different maturity groups, as well as the impact of growing conditions (substrate, temperature and light) on the properties of the colony and the pycnidial formation of this fungus were studied. The large number of symptoms was obtained by various combinations of tissue colour changes (red, brown, yellow, blue and lighter or shades of these colours), changes in a particular part of the root (root top, part of the epidermis, the entire epidermis, tissue under the epidermis or the whole root) and the form of spots and streaks (shape and size). Pinkish red symptoms prevailed on younger roots, particularly from the 5th to the 7th internode. When P. terrestris was grown on potato dextrose agar (PDA) in the dark at 25 degrees C, the variability of the fungus was expressed in relation to the colour of the aerial (white, pink, grey, yellow and brown) and the substrate mycelium (purple, pink, grey, green and yellowish) and to the pycnidial formation (present or absent). The interrelationship between types of symptoms and properties of P. terrestris colonies was not determined on PDA. The fungus always produced the red purple pigment and mostly pycnidia on carnation leaf agar (CLA) at 25 degrees C and under the alternating 12 h combined light (fluorescent and near ultra violet (NUV) light)/dark conditions. These conditions are suitable for the reliable identification of the fungus.
PB  - Natl Agricultural Research & Development Inst, Fundulea
T2  - Romanian Agricultural Research
T1  - Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties
VL  - 29
SP  - 339
EP  - 347
UR  - conv_830
ER  - 
@article{
author = "Lević, Jelena and Petrović, Tijana and Stanković, Slavica and Stanković, Goran and Krnjaja, Vesna",
year = "2012",
abstract = "Rot symptoms that Pyrenochaeta terrestris causes on the root of each internode of maize hybrids belonging to different maturity groups, as well as the impact of growing conditions (substrate, temperature and light) on the properties of the colony and the pycnidial formation of this fungus were studied. The large number of symptoms was obtained by various combinations of tissue colour changes (red, brown, yellow, blue and lighter or shades of these colours), changes in a particular part of the root (root top, part of the epidermis, the entire epidermis, tissue under the epidermis or the whole root) and the form of spots and streaks (shape and size). Pinkish red symptoms prevailed on younger roots, particularly from the 5th to the 7th internode. When P. terrestris was grown on potato dextrose agar (PDA) in the dark at 25 degrees C, the variability of the fungus was expressed in relation to the colour of the aerial (white, pink, grey, yellow and brown) and the substrate mycelium (purple, pink, grey, green and yellowish) and to the pycnidial formation (present or absent). The interrelationship between types of symptoms and properties of P. terrestris colonies was not determined on PDA. The fungus always produced the red purple pigment and mostly pycnidia on carnation leaf agar (CLA) at 25 degrees C and under the alternating 12 h combined light (fluorescent and near ultra violet (NUV) light)/dark conditions. These conditions are suitable for the reliable identification of the fungus.",
publisher = "Natl Agricultural Research & Development Inst, Fundulea",
journal = "Romanian Agricultural Research",
title = "Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties",
volume = "29",
pages = "339-347",
url = "conv_830"
}
Lević, J., Petrović, T., Stanković, S., Stanković, G.,& Krnjaja, V.. (2012). Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties. in Romanian Agricultural Research
Natl Agricultural Research & Development Inst, Fundulea., 29, 339-347.
conv_830
Lević J, Petrović T, Stanković S, Stanković G, Krnjaja V. Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties. in Romanian Agricultural Research. 2012;29:339-347.
conv_830 .
Lević, Jelena, Petrović, Tijana, Stanković, Slavica, Stanković, Goran, Krnjaja, Vesna, "Different symptoms in maize root caused by pyrenochaeta terrestris and the fungal colony properties" in Romanian Agricultural Research, 29 (2012):339-347,
conv_830 .
3
3

Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain

Stanković, Slavica; Lević, Jelena; Ivanović, Dragica; Stanković, Goran; Krnjaja, Vesna; Tančić, Sonja

(Elsevier Sci Ltd, Oxford, 2012)

TY  - JOUR
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Ivanović, Dragica
AU  - Stanković, Goran
AU  - Krnjaja, Vesna
AU  - Tančić, Sonja
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/420
AB  - The natural occurrence of fumonisin B-1 (FB1) and its co-occurrence with zearalenone (ZEA), T-2 toxin and deoxynivalenol (DON) were surveyed in 103 winter wheat samples collected after four to six-month storage in family barns from different locations in Serbia. All 103 samples were mycotoxin positive. The mean concentrations of all mycotoxins except ZEA were greater in 2005 than in 2007. FB1 was detected in 82.1% and 92.0% of all samples with ranges of 750-5400 mu g kg(-1) (mean, 2079.45 mu g kg(-1)) and 750-4900 mu g kg(-1) (mean 918.76 mu g kg(-1)) in 2005 and 2007, respectively. Moderate positive correlations were found between FB1 and DON concentrations (r = 0.56 in 2005 and r = 0.54 in 2007) and between FB1 and ZEA concentrations (r = 0.48 in 2005 and r = 0.60 in 2007), while a moderate negative correlation was detected between the production of FB1 and T-2 toxin in 2007 (r = -0.33). This is the first report of FB1 occurrence in naturally-contaminated wheat grain and its simultaneous occurrence with ZEA, DON and T-2 toxin in Serbia. Moreover, this is one of the rare reports presenting the occurrence of FB1 on wheat in the world.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain
VL  - 23
IS  - 2
SP  - 384
EP  - 388
DO  - 10.1016/j.foodcont.2011.08.003
UR  - conv_794
ER  - 
@article{
author = "Stanković, Slavica and Lević, Jelena and Ivanović, Dragica and Stanković, Goran and Krnjaja, Vesna and Tančić, Sonja",
year = "2012",
abstract = "The natural occurrence of fumonisin B-1 (FB1) and its co-occurrence with zearalenone (ZEA), T-2 toxin and deoxynivalenol (DON) were surveyed in 103 winter wheat samples collected after four to six-month storage in family barns from different locations in Serbia. All 103 samples were mycotoxin positive. The mean concentrations of all mycotoxins except ZEA were greater in 2005 than in 2007. FB1 was detected in 82.1% and 92.0% of all samples with ranges of 750-5400 mu g kg(-1) (mean, 2079.45 mu g kg(-1)) and 750-4900 mu g kg(-1) (mean 918.76 mu g kg(-1)) in 2005 and 2007, respectively. Moderate positive correlations were found between FB1 and DON concentrations (r = 0.56 in 2005 and r = 0.54 in 2007) and between FB1 and ZEA concentrations (r = 0.48 in 2005 and r = 0.60 in 2007), while a moderate negative correlation was detected between the production of FB1 and T-2 toxin in 2007 (r = -0.33). This is the first report of FB1 occurrence in naturally-contaminated wheat grain and its simultaneous occurrence with ZEA, DON and T-2 toxin in Serbia. Moreover, this is one of the rare reports presenting the occurrence of FB1 on wheat in the world.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain",
volume = "23",
number = "2",
pages = "384-388",
doi = "10.1016/j.foodcont.2011.08.003",
url = "conv_794"
}
Stanković, S., Lević, J., Ivanović, D., Stanković, G., Krnjaja, V.,& Tančić, S.. (2012). Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain. in Food Control
Elsevier Sci Ltd, Oxford., 23(2), 384-388.
https://doi.org/10.1016/j.foodcont.2011.08.003
conv_794
Stanković S, Lević J, Ivanović D, Stanković G, Krnjaja V, Tančić S. Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain. in Food Control. 2012;23(2):384-388.
doi:10.1016/j.foodcont.2011.08.003
conv_794 .
Stanković, Slavica, Lević, Jelena, Ivanović, Dragica, Stanković, Goran, Krnjaja, Vesna, Tančić, Sonja, "Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain" in Food Control, 23, no. 2 (2012):384-388,
https://doi.org/10.1016/j.foodcont.2011.08.003 .,
conv_794 .
3
46
43
45

Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root

Lević, Jelena; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra

(Elsevier Sci Ltd, Oxford, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/410
AB  - The present study describes the developed filter layer method for the consistent determination and identification of the natural occurrence of Pyrenochaeta terrestris in maize roots. The field experiment was set up to establish the applicability, accuracy and efficiency of this method for the pathogen isolation from roots of maize grown under different agroecological conditions. According to this method, the 10- to 15-mm long tissue sections, mainly cut at the margin of lesions developed in roots, were placed on two layers of moisten filter paper in Petri dishes and incubated for 6-7 days under laboratory conditions, 25 +/- 2 degrees C and daylight. The unique reddish-purple (beet-red) pigmentation was used as an indicator of the fungal incidence in infected roots, which facilitated a succeeding confirmation of the pathogen on PDA at 25 degrees C in the dark and on CLA in the alternating 12 h light (fluorescent and near-ultraviolet light)/12 h dark conditions. Obtained results showed that the filter layer method was very effective for the P. terrestris detection and that it could be applied for a rapid assessment of response of maize genotypes, environmental and seasonal effects on the fungal growth on roots and the fungal distribution. Advantages of this method for the detection and quantification of P. terrestris in relation to the methods described in the literature include: a) low investments, because many root samples can be tested immediately at the same time; b) results can be obtained rapidly (6-7 days after sampling); and c) minimal interference by other fungi or bacteria.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root
VL  - 38
SP  - 66
EP  - 71
DO  - 10.1016/j.cropro.2012.03.015
UR  - conv_816
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra",
year = "2012",
abstract = "The present study describes the developed filter layer method for the consistent determination and identification of the natural occurrence of Pyrenochaeta terrestris in maize roots. The field experiment was set up to establish the applicability, accuracy and efficiency of this method for the pathogen isolation from roots of maize grown under different agroecological conditions. According to this method, the 10- to 15-mm long tissue sections, mainly cut at the margin of lesions developed in roots, were placed on two layers of moisten filter paper in Petri dishes and incubated for 6-7 days under laboratory conditions, 25 +/- 2 degrees C and daylight. The unique reddish-purple (beet-red) pigmentation was used as an indicator of the fungal incidence in infected roots, which facilitated a succeeding confirmation of the pathogen on PDA at 25 degrees C in the dark and on CLA in the alternating 12 h light (fluorescent and near-ultraviolet light)/12 h dark conditions. Obtained results showed that the filter layer method was very effective for the P. terrestris detection and that it could be applied for a rapid assessment of response of maize genotypes, environmental and seasonal effects on the fungal growth on roots and the fungal distribution. Advantages of this method for the detection and quantification of P. terrestris in relation to the methods described in the literature include: a) low investments, because many root samples can be tested immediately at the same time; b) results can be obtained rapidly (6-7 days after sampling); and c) minimal interference by other fungi or bacteria.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root",
volume = "38",
pages = "66-71",
doi = "10.1016/j.cropro.2012.03.015",
url = "conv_816"
}
Lević, J., Stanković, S., Krnjaja, V.,& Bočarov-Stančić, A.. (2012). Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root. in Crop Protection
Elsevier Sci Ltd, Oxford., 38, 66-71.
https://doi.org/10.1016/j.cropro.2012.03.015
conv_816
Lević J, Stanković S, Krnjaja V, Bočarov-Stančić A. Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root. in Crop Protection. 2012;38:66-71.
doi:10.1016/j.cropro.2012.03.015
conv_816 .
Lević, Jelena, Stanković, Slavica, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra, "Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root" in Crop Protection, 38 (2012):66-71,
https://doi.org/10.1016/j.cropro.2012.03.015 .,
conv_816 .
1
1
1

Relationships of mycobiota on rachides and kernels of wheat

Lević, Jelena; Stanković, Slavica; Ivanović, Dragica; Krnjaja, Vesna; Tančić, Sonja; Bočarov-Stančić, Aleksandra

(Springer, Dordrecht, 2012)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Ivanović, Dragica
AU  - Krnjaja, Vesna
AU  - Tančić, Sonja
AU  - Bočarov-Stančić, Aleksandra
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/418
AB  - The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14 wheat cultivars grown under 19 different agroecological conditions in Serbia, were studied. Out of the 23 identified fungal genera, a significantly higher number of species was isolated and identified from rachides (22) than from kernels of wheat (9). Fusarium and Alternaria species were the most frequent (up to 100 %) species on both, rachides and kernels, but the incidence of these fungi were higher on rachides than on kernels. The most frequent of the 14 Fusarium species were F. graminearum (96.8 % on both, rachides and kernels) and F. poae (93.8 % on rachides and 51.6 % on kernels). The frequency of F. verticillioides was significantly higher on rachides (64.5 %) than on kernels (19.4 %). A positive correlation (r = 0.5356 **) was established between the frequency of F. graminearum on rachides and on kernels. Furthermore, the frequency of Alternaria spp. was also statistically higher on rachides than on kernels, but the correlation was not statistically significant (r = 0.1729). The incidence of F. graminearum was negatively correlated with the incidence of Alternaria species in both, rachides (r = -0.3783 *) and kernels (r = -0.4863 **). These are the first data on the frequency and incidence of fungi on wheat rachides in Serbia, and they support the few data presented in the world literature. Results of this research could be useful for better understanding of pathways in a fungal infection and the improvement of wheat breeding for resistance, as well as, a proper application of fungicides in the wheat head protection.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Relationships of mycobiota on rachides and kernels of wheat
VL  - 134
IS  - 2
SP  - 249
EP  - 256
DO  - 10.1007/s10658-012-9982-8
UR  - conv_824
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Ivanović, Dragica and Krnjaja, Vesna and Tančić, Sonja and Bočarov-Stančić, Aleksandra",
year = "2012",
abstract = "The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14 wheat cultivars grown under 19 different agroecological conditions in Serbia, were studied. Out of the 23 identified fungal genera, a significantly higher number of species was isolated and identified from rachides (22) than from kernels of wheat (9). Fusarium and Alternaria species were the most frequent (up to 100 %) species on both, rachides and kernels, but the incidence of these fungi were higher on rachides than on kernels. The most frequent of the 14 Fusarium species were F. graminearum (96.8 % on both, rachides and kernels) and F. poae (93.8 % on rachides and 51.6 % on kernels). The frequency of F. verticillioides was significantly higher on rachides (64.5 %) than on kernels (19.4 %). A positive correlation (r = 0.5356 **) was established between the frequency of F. graminearum on rachides and on kernels. Furthermore, the frequency of Alternaria spp. was also statistically higher on rachides than on kernels, but the correlation was not statistically significant (r = 0.1729). The incidence of F. graminearum was negatively correlated with the incidence of Alternaria species in both, rachides (r = -0.3783 *) and kernels (r = -0.4863 **). These are the first data on the frequency and incidence of fungi on wheat rachides in Serbia, and they support the few data presented in the world literature. Results of this research could be useful for better understanding of pathways in a fungal infection and the improvement of wheat breeding for resistance, as well as, a proper application of fungicides in the wheat head protection.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Relationships of mycobiota on rachides and kernels of wheat",
volume = "134",
number = "2",
pages = "249-256",
doi = "10.1007/s10658-012-9982-8",
url = "conv_824"
}
Lević, J., Stanković, S., Ivanović, D., Krnjaja, V., Tančić, S.,& Bočarov-Stančić, A.. (2012). Relationships of mycobiota on rachides and kernels of wheat. in European Journal of Plant Pathology
Springer, Dordrecht., 134(2), 249-256.
https://doi.org/10.1007/s10658-012-9982-8
conv_824
Lević J, Stanković S, Ivanović D, Krnjaja V, Tančić S, Bočarov-Stančić A. Relationships of mycobiota on rachides and kernels of wheat. in European Journal of Plant Pathology. 2012;134(2):249-256.
doi:10.1007/s10658-012-9982-8
conv_824 .
Lević, Jelena, Stanković, Slavica, Ivanović, Dragica, Krnjaja, Vesna, Tančić, Sonja, Bočarov-Stančić, Aleksandra, "Relationships of mycobiota on rachides and kernels of wheat" in European Journal of Plant Pathology, 134, no. 2 (2012):249-256,
https://doi.org/10.1007/s10658-012-9982-8 .,
conv_824 .
6
8
9

Fusariotoxins in wheat grain in Serbia

Stepanić, Ana; Stanković, Slavica; Lević, Jelena; Ivanović, Mirko; Krnjaja, Vesna

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2011)

TY  - JOUR
AU  - Stepanić, Ana
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Ivanović, Mirko
AU  - Krnjaja, Vesna
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/399
AB  - Samples of wheat grain (41), collected during the 2010 harvest from seven localities in Serbia, were analyzed for the presence of zearalenone (ZEA), T-2 toxin, deoxynivalenol (DON) and fumonisine B1 (FB1). Results of Enzyme-Linked Immunosorbent Assay (ELISA) showed that all analysed samples were positive for the presence of at least one of four observed fusariotoxins. The most distributed mycotoxins were ZEA (90.2%, with the average concentration of 442.6μg kg-1) and T-2 (90.2%, with the average concentration of 24.2 μg kg-1). DON (73.2%) and FB1 (84.4%) were detected in a somewhat smaller number of samples, but their average concentrations were higher (1988.1 μg DON kg-1 and 882.7 μg FB1 kg-1). The established correlations between concentrations of DON and FB1 (r = 0.32) or DON and ZEA (r = 0.22) were not statistically significant. A negative correlation was established between concentrations of T-2 and FB1 (r= -0.24), as well as, between T-2 and DON (r = -0.36). Detected concentrations of ZEA and T-2 were bellow the level prescribed by the World Health Organisation (WHO), while concentrations of FB1 and DON detected in five that is, 17 samples, respectively, were above the permissible limit for human consumption.
AB  - Uzorci zrna pšenice (41), sakupljeni tokom žetve 2010. godine iz 7 različitih lokaliteta na teritoriji Srbije, analizirani su na prisustvo ZEA, T-2 toksina, deoksinivalenola (DON) i FB1. Primenom ELISA metode utvrđeno je da su svi analizirani uzorci bili pozitivni na prisustvo bar jednog od četiri ispitivana fuzariotoksina. Najzastupljeniji mikotoksini su bili ZEA (90,2%, prosečne koncentracije 442,6 μg kg-1) i T-2 (90,2%, prosečna koncentracija 24,2 kg-1). U nešto manjem broju uzoraka utvrđeni su DON (73,2%) i FB1 (84,4%), ali u većoj prosečnoj koncentraciji (1988,1 μg DON kg-1 i 882,7 μg FB1 kg-1). Nije utvrđena statistički značajna korelacija između koncentracije DON i FB1 (r = 0,32) ili DON i ZEA (r = 0,22). Negativna korelacija je utvrđena između koncentracije T-2 i FB1 (r = -0,24), kao i između T-2 i DON (r = -0,36). Detektovane koncentracije ZEA i T-2 bile su niže od nivoa koji propisuje Svetska zdravstvena organizacija, dok su FB1 i DON detektovani kod 5, odnosno 17 uzoraka u koncentraciji većoj od dozvoljenih u ljudskoj hrani.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Fusariotoxins in wheat grain in Serbia
T1  - Fuzariotoksini u zrnu pšenice u Srbiji
VL  - 26
IS  - 4
SP  - 317
EP  - 323
DO  - 10.2298/PIF1104317S
UR  - conv_602
ER  - 
@article{
author = "Stepanić, Ana and Stanković, Slavica and Lević, Jelena and Ivanović, Mirko and Krnjaja, Vesna",
year = "2011",
abstract = "Samples of wheat grain (41), collected during the 2010 harvest from seven localities in Serbia, were analyzed for the presence of zearalenone (ZEA), T-2 toxin, deoxynivalenol (DON) and fumonisine B1 (FB1). Results of Enzyme-Linked Immunosorbent Assay (ELISA) showed that all analysed samples were positive for the presence of at least one of four observed fusariotoxins. The most distributed mycotoxins were ZEA (90.2%, with the average concentration of 442.6μg kg-1) and T-2 (90.2%, with the average concentration of 24.2 μg kg-1). DON (73.2%) and FB1 (84.4%) were detected in a somewhat smaller number of samples, but their average concentrations were higher (1988.1 μg DON kg-1 and 882.7 μg FB1 kg-1). The established correlations between concentrations of DON and FB1 (r = 0.32) or DON and ZEA (r = 0.22) were not statistically significant. A negative correlation was established between concentrations of T-2 and FB1 (r= -0.24), as well as, between T-2 and DON (r = -0.36). Detected concentrations of ZEA and T-2 were bellow the level prescribed by the World Health Organisation (WHO), while concentrations of FB1 and DON detected in five that is, 17 samples, respectively, were above the permissible limit for human consumption., Uzorci zrna pšenice (41), sakupljeni tokom žetve 2010. godine iz 7 različitih lokaliteta na teritoriji Srbije, analizirani su na prisustvo ZEA, T-2 toksina, deoksinivalenola (DON) i FB1. Primenom ELISA metode utvrđeno je da su svi analizirani uzorci bili pozitivni na prisustvo bar jednog od četiri ispitivana fuzariotoksina. Najzastupljeniji mikotoksini su bili ZEA (90,2%, prosečne koncentracije 442,6 μg kg-1) i T-2 (90,2%, prosečna koncentracija 24,2 kg-1). U nešto manjem broju uzoraka utvrđeni su DON (73,2%) i FB1 (84,4%), ali u većoj prosečnoj koncentraciji (1988,1 μg DON kg-1 i 882,7 μg FB1 kg-1). Nije utvrđena statistički značajna korelacija između koncentracije DON i FB1 (r = 0,32) ili DON i ZEA (r = 0,22). Negativna korelacija je utvrđena između koncentracije T-2 i FB1 (r = -0,24), kao i između T-2 i DON (r = -0,36). Detektovane koncentracije ZEA i T-2 bile su niže od nivoa koji propisuje Svetska zdravstvena organizacija, dok su FB1 i DON detektovani kod 5, odnosno 17 uzoraka u koncentraciji većoj od dozvoljenih u ljudskoj hrani.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Fusariotoxins in wheat grain in Serbia, Fuzariotoksini u zrnu pšenice u Srbiji",
volume = "26",
number = "4",
pages = "317-323",
doi = "10.2298/PIF1104317S",
url = "conv_602"
}
Stepanić, A., Stanković, S., Lević, J., Ivanović, M.,& Krnjaja, V.. (2011). Fusariotoxins in wheat grain in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(4), 317-323.
https://doi.org/10.2298/PIF1104317S
conv_602
Stepanić A, Stanković S, Lević J, Ivanović M, Krnjaja V. Fusariotoxins in wheat grain in Serbia. in Pesticidi i fitomedicina. 2011;26(4):317-323.
doi:10.2298/PIF1104317S
conv_602 .
Stepanić, Ana, Stanković, Slavica, Lević, Jelena, Ivanović, Mirko, Krnjaja, Vesna, "Fusariotoxins in wheat grain in Serbia" in Pesticidi i fitomedicina, 26, no. 4 (2011):317-323,
https://doi.org/10.2298/PIF1104317S .,
conv_602 .
6

Fusarium species and their mycotoxins in wheat grain

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Stepanić, Ana

(Matica srpska, Novi Sad, 2011)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Stepanić, Ana
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/378
AB  - Incidence of fungi and concentration of mycotoxin deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the grain of the winter wheat collected subsequently after harvesting in 2010. In the analyzed samples the highest incidence was determined for the species of Fusarium (84.7%) genus, significantly lower incidence was determined for the species of genus Alternaria (12.35%), and especially for species of genera Acremoniella (2.05%), Acremonium (0.65%) and Penicillium (0.25%). F. graminearum (99.05%) was the most present species of Fusarium genus, whereas the following species F. sporotrichioides (0.4%), F. subglutinans (0.4%), F. poae (0.05%), F. proliferatum (0.05%) and F. verticillioides (0.05%) were only sporadic. The presence of DON, ZON and FB1 mycotoxins was established in all studied wheat samples. DON was detected in concentrations from 123 to 393 μg kg-1 (average 214 μg kg-1), ZON from 157.144 to 471.055 μg kg-1 (average 299.934 μg kg-1), and FB1 from 2.715 to 16.488 μg kg-1 (average 6.286 μg kg-1).
AB  - Učestalost gljiva i koncentracija mikotoksina deoksinivalenola (DON), zearalenona (ZON) i fumonizina (FB1) proučavana je u zrnu ozime pšenice prikupljene neposredno nakon žetve u 2010. godini. U ispitivanim uzorcima najveću zastupljenost imaju vrste iz roda Fusarium (84,7%), a znatno manje vrste iz roda Alternaria (12,35%), a posebno iz rodova Acremoniella (2,05%), Acremonium (0,65%) i Penicillium (0,25%). Od vrsta roda Fusarium najzastupljenija je vrsta F. graminearum (99,05%), dok su sporadične vrste F. sporotrichioides (0,4%), F. subglutinans (0,4%), F. poae (0,05%), F. proliferatum (0,05%) i F. verticillioides (0,05%). Prisustvo mikotoksina DON, ZON i FB1 ustanovljeno je u svim ispitivanim uzorcima zrna pšenice. DON je bio detektovan u koncentracijama od 123 do 393 μg kg-1 (prosek 214 μg kg-1), ZON od 157,144 do 471,055 μg kg-1 (prosek 299,934 μg kg-1) i FB1 od 2,715 do 16,488 μg kg-1 (prosek 6,286 μg kg-1).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Fusarium species and their mycotoxins in wheat grain
T1  - Fusarium vrste i njihovi mikotoksini u zrnu pšenice
IS  - 120
SP  - 41
EP  - 48
DO  - 10.2298/ZMSPN1120041K
UR  - conv_56
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Stepanić, Ana",
year = "2011",
abstract = "Incidence of fungi and concentration of mycotoxin deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the grain of the winter wheat collected subsequently after harvesting in 2010. In the analyzed samples the highest incidence was determined for the species of Fusarium (84.7%) genus, significantly lower incidence was determined for the species of genus Alternaria (12.35%), and especially for species of genera Acremoniella (2.05%), Acremonium (0.65%) and Penicillium (0.25%). F. graminearum (99.05%) was the most present species of Fusarium genus, whereas the following species F. sporotrichioides (0.4%), F. subglutinans (0.4%), F. poae (0.05%), F. proliferatum (0.05%) and F. verticillioides (0.05%) were only sporadic. The presence of DON, ZON and FB1 mycotoxins was established in all studied wheat samples. DON was detected in concentrations from 123 to 393 μg kg-1 (average 214 μg kg-1), ZON from 157.144 to 471.055 μg kg-1 (average 299.934 μg kg-1), and FB1 from 2.715 to 16.488 μg kg-1 (average 6.286 μg kg-1)., Učestalost gljiva i koncentracija mikotoksina deoksinivalenola (DON), zearalenona (ZON) i fumonizina (FB1) proučavana je u zrnu ozime pšenice prikupljene neposredno nakon žetve u 2010. godini. U ispitivanim uzorcima najveću zastupljenost imaju vrste iz roda Fusarium (84,7%), a znatno manje vrste iz roda Alternaria (12,35%), a posebno iz rodova Acremoniella (2,05%), Acremonium (0,65%) i Penicillium (0,25%). Od vrsta roda Fusarium najzastupljenija je vrsta F. graminearum (99,05%), dok su sporadične vrste F. sporotrichioides (0,4%), F. subglutinans (0,4%), F. poae (0,05%), F. proliferatum (0,05%) i F. verticillioides (0,05%). Prisustvo mikotoksina DON, ZON i FB1 ustanovljeno je u svim ispitivanim uzorcima zrna pšenice. DON je bio detektovan u koncentracijama od 123 do 393 μg kg-1 (prosek 214 μg kg-1), ZON od 157,144 do 471,055 μg kg-1 (prosek 299,934 μg kg-1) i FB1 od 2,715 do 16,488 μg kg-1 (prosek 6,286 μg kg-1).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Fusarium species and their mycotoxins in wheat grain, Fusarium vrste i njihovi mikotoksini u zrnu pšenice",
number = "120",
pages = "41-48",
doi = "10.2298/ZMSPN1120041K",
url = "conv_56"
}
Krnjaja, V., Lević, J., Stanković, S.,& Stepanić, A.. (2011). Fusarium species and their mycotoxins in wheat grain. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(120), 41-48.
https://doi.org/10.2298/ZMSPN1120041K
conv_56
Krnjaja V, Lević J, Stanković S, Stepanić A. Fusarium species and their mycotoxins in wheat grain. in Zbornik Matice srpske za prirodne nauke. 2011;(120):41-48.
doi:10.2298/ZMSPN1120041K
conv_56 .
Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica, Stepanić, Ana, "Fusarium species and their mycotoxins in wheat grain" in Zbornik Matice srpske za prirodne nauke, no. 120 (2011):41-48,
https://doi.org/10.2298/ZMSPN1120041K .,
conv_56 .
4

In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate

Bočarov-Stančić, Aleksandra; Stanković, Slavica; Lević, Jelena; Salma, Nataša M.; Pantić, Vladimir R.; Barnić, Saša S.

(Matica srpska, Novi Sad, 2011)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Salma, Nataša M.
AU  - Pantić, Vladimir R.
AU  - Barnić, Saša S.
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/383
AB  - Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium. According to previously performed experiments it was proved that the selected isolate, originating from sunflower meal, had the ability to degrade these fusariotoxins when growing on the modified Vogel's agar supplemented with crude extracts of DAS and T-2 toxin. In order to determine biodegradation of fusariotoxins, the liquid nutritive medium - SPY (5% sucrose + 0.1% peptone + 0.1% yeast extract, pH 6.2) was simultaneously inoculated with the isolate M. racemosus f. racemosus 1215/09 and: a) Fusarium semitectum SL-B (DAS producer) or b) F. sporotrichioides R-2301 (T-2 toxin producer). The SPY media, inoculated with single fungal isolates, were used as a control of toxin biosynthesis. The cultures were incubated at room temperature (21-26ºC) on the rotary shaker (175 rpm). After the 3-5-day incubation, the filtration of liquid cultures and the extraction of fusariotoxins from filtrates with ethyl-acetate were performed. Determinations of DAS and T-2 toxin were done by thin layer chromatography using silica gel G. Depending on the incubation duration, M. racemosus f. racemosus in the mixed culture with F. semitectum degraded from 90.0 to 99.97% of DAS present in the medium (40,000- 120,000 µg l-1), while in the mixed culture with F. sporotrichioides it degraded from 95.0 to 96.7% of T-2 toxin present in the medium (240,000 µg l-1). Sterile filtrates of mixed cultures and single culture of M. racemosus f. racemosus, obtained by passing liquid cultures through the 0.45-µm membrane filter and added to the SPY medium, did not affect degradation of type A trichothecenes that had been biosynthesised by isolates F. semitectum SL-B and F. sporotrichioides R-2301 in the liquid medium.
AB  - U kontrolisanim in vitro uslovima proučavana je sposobnost izolata Mucor racemosus f. racemosus 1215/09 da degraduje trihotecene tipa A (diacetoksiscirpenol - DAS i T-2 toksin) u tečnoj hranljivoj podlozi. Prethodnim eksperimentima je dokazano da odabrani izolat, poreklom sa suncokretove sačme, poseduje sposobnost razgradnje navedenih fuzariotoksina, koji su kao sirovi ekstrakti dodati u modifikovanu Vogelovu podlogu. U cilju utvrđivanja biodegradacije fuzariotoksina tečna hranljiva podloga SPK (5% saharoza + 0,1% pepton + 0,1% ekstrakt kvasca, pH 6,2) je zasejana u isto vreme izolatom M. racemosus f. racemosus 1215/09 i: a) Fusarium semitectum SL-B (proizvođač DAS-a) ili b) F. sporotrichioides R-2301 (proizvođač T-2 toksina). Kao kontrola biosinteze toksina korišćena je SPK podloga inokulisana pojedinačnim izolatima gljiva. Kulture su inkubirane na rotacionoj tresilici (175 o/min) tokom 3-5 dana na sobnoj temperaturi (21-26ºC). Nakon 3 do 5 dana inkubacije vršeno je filtriranje tečnih kultura i ekstrakcija fuzariotoksina iz filtrata etil-acetatom. Determinacija DAS-a i T-2 toksina je rađena tankoslojnom hromatografijom na silika gelu G. Zavisno od dužine inkubacije, M. racemosus f. racemosus je u združenoj kulturi sa F. semitectum degradovala 90,0-99,97% DAS-a prisutnog u podlozi (40.000-120.000 µg l-1), dok je u združenoj kulturi sa F. sporotrichioides razgradila 95,0-96,7% T-2 toksina prisutnog u podlozi (240.000 µg l-1). Sterilni filtrati mešanih kultura i pojedinačne kulture M. racemosus f. racemosus, dobijeni propuštanjem tečnih kultura kroz 0,45 µm membranski filter i dodati SPK podlozi, nisu uticali na razgradnju trihotecena tipa A koje su biosintetisali izolati F. semitectum SL-B i F. sporotrichioides R-2301 u tečnoj podlozi.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate
T1  - In vitro degradacija diacetoksiscirpenola i T-2 toksina posredstvom izolata Mucor racemosus fresen. f. racemosus
IS  - 121
SP  - 51
EP  - 59
DO  - 10.2298/ZMSPN1121051B
UR  - conv_58
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Stanković, Slavica and Lević, Jelena and Salma, Nataša M. and Pantić, Vladimir R. and Barnić, Saša S.",
year = "2011",
abstract = "Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium. According to previously performed experiments it was proved that the selected isolate, originating from sunflower meal, had the ability to degrade these fusariotoxins when growing on the modified Vogel's agar supplemented with crude extracts of DAS and T-2 toxin. In order to determine biodegradation of fusariotoxins, the liquid nutritive medium - SPY (5% sucrose + 0.1% peptone + 0.1% yeast extract, pH 6.2) was simultaneously inoculated with the isolate M. racemosus f. racemosus 1215/09 and: a) Fusarium semitectum SL-B (DAS producer) or b) F. sporotrichioides R-2301 (T-2 toxin producer). The SPY media, inoculated with single fungal isolates, were used as a control of toxin biosynthesis. The cultures were incubated at room temperature (21-26ºC) on the rotary shaker (175 rpm). After the 3-5-day incubation, the filtration of liquid cultures and the extraction of fusariotoxins from filtrates with ethyl-acetate were performed. Determinations of DAS and T-2 toxin were done by thin layer chromatography using silica gel G. Depending on the incubation duration, M. racemosus f. racemosus in the mixed culture with F. semitectum degraded from 90.0 to 99.97% of DAS present in the medium (40,000- 120,000 µg l-1), while in the mixed culture with F. sporotrichioides it degraded from 95.0 to 96.7% of T-2 toxin present in the medium (240,000 µg l-1). Sterile filtrates of mixed cultures and single culture of M. racemosus f. racemosus, obtained by passing liquid cultures through the 0.45-µm membrane filter and added to the SPY medium, did not affect degradation of type A trichothecenes that had been biosynthesised by isolates F. semitectum SL-B and F. sporotrichioides R-2301 in the liquid medium., U kontrolisanim in vitro uslovima proučavana je sposobnost izolata Mucor racemosus f. racemosus 1215/09 da degraduje trihotecene tipa A (diacetoksiscirpenol - DAS i T-2 toksin) u tečnoj hranljivoj podlozi. Prethodnim eksperimentima je dokazano da odabrani izolat, poreklom sa suncokretove sačme, poseduje sposobnost razgradnje navedenih fuzariotoksina, koji su kao sirovi ekstrakti dodati u modifikovanu Vogelovu podlogu. U cilju utvrđivanja biodegradacije fuzariotoksina tečna hranljiva podloga SPK (5% saharoza + 0,1% pepton + 0,1% ekstrakt kvasca, pH 6,2) je zasejana u isto vreme izolatom M. racemosus f. racemosus 1215/09 i: a) Fusarium semitectum SL-B (proizvođač DAS-a) ili b) F. sporotrichioides R-2301 (proizvođač T-2 toksina). Kao kontrola biosinteze toksina korišćena je SPK podloga inokulisana pojedinačnim izolatima gljiva. Kulture su inkubirane na rotacionoj tresilici (175 o/min) tokom 3-5 dana na sobnoj temperaturi (21-26ºC). Nakon 3 do 5 dana inkubacije vršeno je filtriranje tečnih kultura i ekstrakcija fuzariotoksina iz filtrata etil-acetatom. Determinacija DAS-a i T-2 toksina je rađena tankoslojnom hromatografijom na silika gelu G. Zavisno od dužine inkubacije, M. racemosus f. racemosus je u združenoj kulturi sa F. semitectum degradovala 90,0-99,97% DAS-a prisutnog u podlozi (40.000-120.000 µg l-1), dok je u združenoj kulturi sa F. sporotrichioides razgradila 95,0-96,7% T-2 toksina prisutnog u podlozi (240.000 µg l-1). Sterilni filtrati mešanih kultura i pojedinačne kulture M. racemosus f. racemosus, dobijeni propuštanjem tečnih kultura kroz 0,45 µm membranski filter i dodati SPK podlozi, nisu uticali na razgradnju trihotecena tipa A koje su biosintetisali izolati F. semitectum SL-B i F. sporotrichioides R-2301 u tečnoj podlozi.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate, In vitro degradacija diacetoksiscirpenola i T-2 toksina posredstvom izolata Mucor racemosus fresen. f. racemosus",
number = "121",
pages = "51-59",
doi = "10.2298/ZMSPN1121051B",
url = "conv_58"
}
Bočarov-Stančić, A., Stanković, S., Lević, J., Salma, N. M., Pantić, V. R.,& Barnić, S. S.. (2011). In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(121), 51-59.
https://doi.org/10.2298/ZMSPN1121051B
conv_58
Bočarov-Stančić A, Stanković S, Lević J, Salma NM, Pantić VR, Barnić SS. In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate. in Zbornik Matice srpske za prirodne nauke. 2011;(121):51-59.
doi:10.2298/ZMSPN1121051B
conv_58 .
Bočarov-Stančić, Aleksandra, Stanković, Slavica, Lević, Jelena, Salma, Nataša M., Pantić, Vladimir R., Barnić, Saša S., "In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate" in Zbornik Matice srpske za prirodne nauke, no. 121 (2011):51-59,
https://doi.org/10.2298/ZMSPN1121051B .,
conv_58 .
2

Identification of Tilletia species using rep-PCR fingerprinting technique

Župunski, Vesna; Ignjatović-Micić, Dragana; Nikolić, Ana; Stanković, Slavica; Jevtić, Radivoje; Lević, Jelena; Ivanović, Dragica

(Društvo genetičara Srbije, Beograd, 2011)

TY  - JOUR
AU  - Župunski, Vesna
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Ana
AU  - Stanković, Slavica
AU  - Jevtić, Radivoje
AU  - Lević, Jelena
AU  - Ivanović, Dragica
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/370
AB  - Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %) samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas.
AB  - Istraživanjem kontaminiranosti uzoraka semena pšenice teleutosporama Tilletia vrsta, utvrđeno je da je od 167 uzoraka nedorađenog semena pšenice, bilo kontaminirano 145 (86,8%), dok su 22 uzorka (13,2%) smatrana nekontaminiranim. Identifikacija Tilletia vrsta izvršena je rep-PCR fingerprinting tehnikom. Izolati prikupljeni na teritoriji Republike Srbije identifikovani su kao T. tritici, s obzirom da su sa pozitivnom kontrolom imali genetičku sličnost veću od 80%. Jedan od izolata koji je vodio poreklo iz opštine Apatin bio je oko 60% genetički sličan sa izolatima T. tritici, T. controversa i T. laevis. Pretpostavljeno je da pripada vrsti T. bromi. Genetička sličnost izolata T. walkeri i vrsta: T. tritici, T. laevis i T. controversa, iznosila je nešto više od 10%. Genetička sličnost T. tritici, T. controversa i T. laevis bila je oko 70 %. I pored visokog procenta genetičke sličnosti između T. controversa i T. tritici, napravljena je razlika među njima, što rep-PCR fingerprinting tehniku čini veoma podesnom za praćenje prisustva morfološki sličnih Tilleria vrsta prilikom kontrole kvaliteta semena pšenice.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Identification of Tilletia species using rep-PCR fingerprinting technique
T1  - Identifikacija vrsta roda Tilletia rep-PCR fingerprinting tehnikom
VL  - 43
IS  - 1
SP  - 183
EP  - 195
DO  - 10.2298/GENSR1101183Z
UR  - conv_452
ER  - 
@article{
author = "Župunski, Vesna and Ignjatović-Micić, Dragana and Nikolić, Ana and Stanković, Slavica and Jevtić, Radivoje and Lević, Jelena and Ivanović, Dragica",
year = "2011",
abstract = "Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %) samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas., Istraživanjem kontaminiranosti uzoraka semena pšenice teleutosporama Tilletia vrsta, utvrđeno je da je od 167 uzoraka nedorađenog semena pšenice, bilo kontaminirano 145 (86,8%), dok su 22 uzorka (13,2%) smatrana nekontaminiranim. Identifikacija Tilletia vrsta izvršena je rep-PCR fingerprinting tehnikom. Izolati prikupljeni na teritoriji Republike Srbije identifikovani su kao T. tritici, s obzirom da su sa pozitivnom kontrolom imali genetičku sličnost veću od 80%. Jedan od izolata koji je vodio poreklo iz opštine Apatin bio je oko 60% genetički sličan sa izolatima T. tritici, T. controversa i T. laevis. Pretpostavljeno je da pripada vrsti T. bromi. Genetička sličnost izolata T. walkeri i vrsta: T. tritici, T. laevis i T. controversa, iznosila je nešto više od 10%. Genetička sličnost T. tritici, T. controversa i T. laevis bila je oko 70 %. I pored visokog procenta genetičke sličnosti između T. controversa i T. tritici, napravljena je razlika među njima, što rep-PCR fingerprinting tehniku čini veoma podesnom za praćenje prisustva morfološki sličnih Tilleria vrsta prilikom kontrole kvaliteta semena pšenice.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Identification of Tilletia species using rep-PCR fingerprinting technique, Identifikacija vrsta roda Tilletia rep-PCR fingerprinting tehnikom",
volume = "43",
number = "1",
pages = "183-195",
doi = "10.2298/GENSR1101183Z",
url = "conv_452"
}
Župunski, V., Ignjatović-Micić, D., Nikolić, A., Stanković, S., Jevtić, R., Lević, J.,& Ivanović, D.. (2011). Identification of Tilletia species using rep-PCR fingerprinting technique. in Genetika
Društvo genetičara Srbije, Beograd., 43(1), 183-195.
https://doi.org/10.2298/GENSR1101183Z
conv_452
Župunski V, Ignjatović-Micić D, Nikolić A, Stanković S, Jevtić R, Lević J, Ivanović D. Identification of Tilletia species using rep-PCR fingerprinting technique. in Genetika. 2011;43(1):183-195.
doi:10.2298/GENSR1101183Z
conv_452 .
Župunski, Vesna, Ignjatović-Micić, Dragana, Nikolić, Ana, Stanković, Slavica, Jevtić, Radivoje, Lević, Jelena, Ivanović, Dragica, "Identification of Tilletia species using rep-PCR fingerprinting technique" in Genetika, 43, no. 1 (2011):183-195,
https://doi.org/10.2298/GENSR1101183Z .,
conv_452 .
6
6
7

The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A

Bočarov-Stančić, Aleksandra; Lević, Jelena; Salma, Nataša M.; Stanković, Slavica; Pantić, Vladimir R.; Dolić, Bisera J.

(Matica srpska, Novi Sad, 2011)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Salma, Nataša M.
AU  - Stanković, Slavica
AU  - Pantić, Vladimir R.
AU  - Dolić, Bisera J.
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/386
AB  - Nine isolates of fungi of genera Aspergillus, Fusarium, Paecilomyces and Penicillium were cultured on the modified Vogel's medium with the addition of crude ochratoxin A (OTA) extract. This crude OTA extract was derived from a natural solid substrate on which Aspergillus ochraceus strain CBS 108.08 was cultivated. OTA was isolated, partially purified, dried by evaporating and dissolved in ethanol (1 mg ml-1), and added to the test medium up to the final concentration of 10 μg ml-1. The presence of OTA residues was determined after 7 and 14 day cultivation of fungi in the test medium at 27±1°C. The Paecilomyces lilacinus isolate (Inf. 2/A), which completely degraded OTA (150 μg) after only seven days, was selected for further studies. Wet sterile rice grains (50 g + 25 ml distilled water) were inoculated with individual isolates of fungi A. ochraceus (strain CBS 108.08) and P. lilacinus (isolate Inf. 2/A), and with their combination. In the case of P. lilacinus monoculture, 0.9 mg of crude OTA was also added into cultivation substrate. Each test was done in three replications. After the four week cultivation of individual and combined fungi at 27±1°C, inoculated rice grains were dried to the constant weight and pulverized. OTA was determined in these samples by the application of standard TLC method for fodder analysis. OTA in the amount of 61.310 μg kg-1 dry matter (DM) was determined only in the samples inoculated with a producer of ochratoxin A (A. ochraceus, strain CBS 108.08). On the other hand, a much smaller amount of OTA (80 μg kg-1 DM) was detected in samples inoculated with combined cultures of A. ochraceus and P. lilacinus isolates. Gained results indicate that P. lilacinus degraded, on average, 99.8% of OTA. After four week cultivation, the same fungal isolate in the samples of wet sterile rice kernels with the addition of 0.9 mg of crude OTA, completely degraded added crude OTA ( lt 8 μg kg-1).
AB  - Devet izolata gljiva iz rodova Aspergillus, Fusarium, Paecilomyces i Penicillium gajeno je na modifikovanoj Vogelovoj podlozi sa dodatkom sirovog ekstrakta ohratoksina A (OTA). Sirovi ekstrakt OTA je dobijen iz čvrstog prirodnog supstrata na kojem je gajen soj Aspergillus ochraceus CBS 108.08. Izolovan i delimično prečišćen OTA, uparen do suvog ostatka i rastvoren u etanolu (1 mg ml-1), dodat je u test podlogu do finalne koncentracije 10 μg ml-1. Nakon sedam i 14 dana gajenja kultura gljiva u test podlozi na 27 ± 1°C de terminisano je prisustvo rezidua OTA primenom modifikovane metode Filtenborg-a i sar. (1983). Od devet testiranih izolata za dalja ispitivanja je odabran izolat Paecilomyces lilacinus (Inf. 2/A), koji je već posle sedam dana u potpunosti razgradio inicijalnu količinu OTA (150 μg). U drugom delu eksperimenta vlažno sterilno zrno pirinča (50 g + 25 ml destilovane vode) zasejano je sa pojedinačnim izolatima A. ochraceus (CBS 108.08) i P. lilacinus (Inf. 2-A), kao i kombinacijom oba izolata. U slučaju mono-kulture P. lilacinus u podlogu je dodat i sirovi OTA (0,9 mg). Svaki od testova je urađen u 3 ponavljanja. Nakon četiri nedelje gajenja monokultura i mešanih kul tura gljiva na 27±1°C, inokulisana zrna su osušena do konstantne težine i sa mlevena do finog praha. U ovim uzorcima izvršena je determinacija OTA primenom standardne metode tankoslojne hromatografije za analizu stočne hrane. U uzorcima koji su bili zasejani samo sa producentom OTA (A. ochraceus, soj CBS 108.08) detektovan je OTA u prosečnoj količini od 61.310 μg kg-1 suvog ostatka. U uzorcima koji su bili zasejani kombinovanim kulturama izolata A. ochraceus i P. lilacinus utvrđena je znatno manja prosečna količina OTA (80 μg kg-1). Ovi rezultati ukazuju da je izolat P. lilacinus razgradio prosečno 99,8% OTA prisutnog u podlozi za kultivaciju. U uzorcima vlažnog sterilnog zrna pirinča sa dodatkom 0,9 mg sirovog OTA isti gljivični izolat je posle četiri nedelje kultivacije kompletno biorazgradio dodat sirovi OTA ( lt 8 μg kg-1).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A
T1  - Uloga Paecilomyces lilacinus (Thom) Samson i drugih vrsta gljiva u biodegradaciji ohratoksina A
IS  - 120
SP  - 103
EP  - 110
DO  - 10.2298/ZMSPN1120103B
UR  - conv_57
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Salma, Nataša M. and Stanković, Slavica and Pantić, Vladimir R. and Dolić, Bisera J.",
year = "2011",
abstract = "Nine isolates of fungi of genera Aspergillus, Fusarium, Paecilomyces and Penicillium were cultured on the modified Vogel's medium with the addition of crude ochratoxin A (OTA) extract. This crude OTA extract was derived from a natural solid substrate on which Aspergillus ochraceus strain CBS 108.08 was cultivated. OTA was isolated, partially purified, dried by evaporating and dissolved in ethanol (1 mg ml-1), and added to the test medium up to the final concentration of 10 μg ml-1. The presence of OTA residues was determined after 7 and 14 day cultivation of fungi in the test medium at 27±1°C. The Paecilomyces lilacinus isolate (Inf. 2/A), which completely degraded OTA (150 μg) after only seven days, was selected for further studies. Wet sterile rice grains (50 g + 25 ml distilled water) were inoculated with individual isolates of fungi A. ochraceus (strain CBS 108.08) and P. lilacinus (isolate Inf. 2/A), and with their combination. In the case of P. lilacinus monoculture, 0.9 mg of crude OTA was also added into cultivation substrate. Each test was done in three replications. After the four week cultivation of individual and combined fungi at 27±1°C, inoculated rice grains were dried to the constant weight and pulverized. OTA was determined in these samples by the application of standard TLC method for fodder analysis. OTA in the amount of 61.310 μg kg-1 dry matter (DM) was determined only in the samples inoculated with a producer of ochratoxin A (A. ochraceus, strain CBS 108.08). On the other hand, a much smaller amount of OTA (80 μg kg-1 DM) was detected in samples inoculated with combined cultures of A. ochraceus and P. lilacinus isolates. Gained results indicate that P. lilacinus degraded, on average, 99.8% of OTA. After four week cultivation, the same fungal isolate in the samples of wet sterile rice kernels with the addition of 0.9 mg of crude OTA, completely degraded added crude OTA ( lt 8 μg kg-1)., Devet izolata gljiva iz rodova Aspergillus, Fusarium, Paecilomyces i Penicillium gajeno je na modifikovanoj Vogelovoj podlozi sa dodatkom sirovog ekstrakta ohratoksina A (OTA). Sirovi ekstrakt OTA je dobijen iz čvrstog prirodnog supstrata na kojem je gajen soj Aspergillus ochraceus CBS 108.08. Izolovan i delimično prečišćen OTA, uparen do suvog ostatka i rastvoren u etanolu (1 mg ml-1), dodat je u test podlogu do finalne koncentracije 10 μg ml-1. Nakon sedam i 14 dana gajenja kultura gljiva u test podlozi na 27 ± 1°C de terminisano je prisustvo rezidua OTA primenom modifikovane metode Filtenborg-a i sar. (1983). Od devet testiranih izolata za dalja ispitivanja je odabran izolat Paecilomyces lilacinus (Inf. 2/A), koji je već posle sedam dana u potpunosti razgradio inicijalnu količinu OTA (150 μg). U drugom delu eksperimenta vlažno sterilno zrno pirinča (50 g + 25 ml destilovane vode) zasejano je sa pojedinačnim izolatima A. ochraceus (CBS 108.08) i P. lilacinus (Inf. 2-A), kao i kombinacijom oba izolata. U slučaju mono-kulture P. lilacinus u podlogu je dodat i sirovi OTA (0,9 mg). Svaki od testova je urađen u 3 ponavljanja. Nakon četiri nedelje gajenja monokultura i mešanih kul tura gljiva na 27±1°C, inokulisana zrna su osušena do konstantne težine i sa mlevena do finog praha. U ovim uzorcima izvršena je determinacija OTA primenom standardne metode tankoslojne hromatografije za analizu stočne hrane. U uzorcima koji su bili zasejani samo sa producentom OTA (A. ochraceus, soj CBS 108.08) detektovan je OTA u prosečnoj količini od 61.310 μg kg-1 suvog ostatka. U uzorcima koji su bili zasejani kombinovanim kulturama izolata A. ochraceus i P. lilacinus utvrđena je znatno manja prosečna količina OTA (80 μg kg-1). Ovi rezultati ukazuju da je izolat P. lilacinus razgradio prosečno 99,8% OTA prisutnog u podlozi za kultivaciju. U uzorcima vlažnog sterilnog zrna pirinča sa dodatkom 0,9 mg sirovog OTA isti gljivični izolat je posle četiri nedelje kultivacije kompletno biorazgradio dodat sirovi OTA ( lt 8 μg kg-1).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A, Uloga Paecilomyces lilacinus (Thom) Samson i drugih vrsta gljiva u biodegradaciji ohratoksina A",
number = "120",
pages = "103-110",
doi = "10.2298/ZMSPN1120103B",
url = "conv_57"
}
Bočarov-Stančić, A., Lević, J., Salma, N. M., Stanković, S., Pantić, V. R.,& Dolić, B. J.. (2011). The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(120), 103-110.
https://doi.org/10.2298/ZMSPN1120103B
conv_57
Bočarov-Stančić A, Lević J, Salma NM, Stanković S, Pantić VR, Dolić BJ. The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A. in Zbornik Matice srpske za prirodne nauke. 2011;(120):103-110.
doi:10.2298/ZMSPN1120103B
conv_57 .
Bočarov-Stančić, Aleksandra, Lević, Jelena, Salma, Nataša M., Stanković, Slavica, Pantić, Vladimir R., Dolić, Bisera J., "The role of Paecilomyces lilacinus (Thom) Samson and other fungal species in biodegradation of ochratoxin A" in Zbornik Matice srpske za prirodne nauke, no. 120 (2011):103-110,
https://doi.org/10.2298/ZMSPN1120103B .,
conv_57 .
1