@article{
author = "Štrbanović, Ratibor and Stanisavljević, Rade and Poštić, Dobrivoj and Jovanović, Snežana and Tabaković, Marijenka",
year = "2018",
abstract = "Lucerka je, posle kukuruza, najvaţnija krmna vrsta u našoj zemlji,
zahvaljujući ne samo povoljnom hemijskom sastavu i visokom sadrţaju
proteina, već i visokim prinosima i veoma dobrim biološkim osobinama.
Plava lucerka, Medicago sativa L. (2n = 32) je prirodni tetraploid. Cilj ovog
rada je da se ispitivanjem deset razliĉitih sorata lucerke odredi varijabilnost i
grupisanje srodnih genotipova lucerke primenom RAPD molekularnih
markera. Za ekstrakciju DNK iz biljnog materijala korišćeni su prvi zeleni
listići klijanaca lucerke (kotiledoni). Za analizu podataka dobijenih
molekularnom karakterizacijom korišćeni su statistiĉki programi za
populaciono-genetiĉke analize. Primećeno je da se u RAPD analizi genoma
biljaka mogu pratiti repetativne sekvence koje ĉine znaĉajan deo
genetiĉkog materijala. Lanĉana reakcija polimeraze bazirana na
repetativnim sekvencama (RAPD-PCR) korišćena je za utvrđivanje
genetiĉke raznovrsnosti između razliĉitih sorata lucerke. OPA 01, OPA 02,
OPA 13 i OPB 10 prajmeri su korišćeni da bi se dobili razliĉiti DNK profili.
Na osnovu RAPD DNK profila moţe se videti razdvajanje DNK fragmenata
razliĉite veliĉine od 100 bp do 3 kb. Razlike između fragmenata su
ocenjivane vizuelno na osnovu njihovog položaja na gelu. Na gelu na kome
se nalaze razliĉite sorte lucerke sa prajmerom OPB 10 mogu se uoĉiti
razlike između sorata lucerke. Jasno se vidi da se sorta lucerke Zajeĉarska
83 razlikuje od svih ostalih sorata lucerke. Sorte Osjeĉka 99, Osjeĉka 88 i
Osjeĉka 66 pokazuju visok nivo međusobne sliĉnosti, kao i sorte
Kruševaĉka 22 i Kruševaĉka 28, što je i oĉekivano sa obzirom da su
navedene sorte koje su sliĉne selekcionisane u istim selekcionim kućama.
Ovo istraţivanje je potvrdilo da prouĉavana kolekcija sorti lucerke poseduje
varijabilnost neophodnu za uspešan selekcioni proces., After maize, alfalfa is the most important forage species in our
country due to favorable chemical composition and high content of
protein. It is also characterized with high yields and very good
biological properties. Blue alfalfa, Medicago sativa L. (2n = 32) is a
natural tetraploid. The aim of this work was to determine variability
and grouping related alfalfa genotypes using RAPD molecular
markers, by testing ten different alfalfa cultivars. The first green
leaves of alfalfa seedlings (cotyledons) were used for DNA extraction
from the plant material. For the analysis of data obtained by the
molecular characterization the statistical programs for population genetic analysis were used. It is observed that in the RAPD analysis
of the genome can be monitored repetitive sequences that make up
a significant part of the genetic material. The polymerase chain
reaction based on repetitive sequences (RAPD-PCR) was used to
determine the genetic diversity among the different cultivars of
alfalfa. OPA 01, OPA 02, OPA 13, and OPB 10 primers were used to
obtain the various profiles of DNA. Based on RAPD DNA profiles,
the separation of DNA fragments of different size from 100 bp to the
3 kb were noticed. The differences between the fragments were
evaluated visually on the basis of their position on the gel. On the gel
containing different varieties of alfalfa with primer OPB 10,
differences between alfalfa varieties were noticed. It is clear that the
cultivar Zajeĉarska 83 differs from all other cultivars of alfalfa.
Cultivars Osjeĉka 99, Osjeĉka 88, and Osjeĉka 66 showed a high
level of similarity, as well as cultivars Kruševaĉka 22 and
Kruševaĉka 28. This is expected, taking into account that these two
groups of similar cultivars were selected in the same companies.
This study confirmed that studied collection of alfalfa cultivars has
variability necessary for successful selection process.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "6. simpozijum sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjaĉka Banja, 7–11.5.2018. - Zbornik izvoda",
title = "Identifikacija različitih sorata lucerke primenom molekularnih markera, Identification of differeent alfalfa cultivars application of molecular markers",
pages = "184-185",
url = "https://hdl.handle.net/21.15107/rcub_rik_935"
}
