TY  - JOUR
AU  - Oro, Violeta
AU  - Stanisavljević, Rade
AU  - Nikolić, Bogdan
AU  - Tabaković, Marijenka
AU  - Sečanski, Mile
AU  - Solveig, Tosi
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/851
AB  - Cereals, particularly wheat, are staple food of the people from the Balkans, dating back
to the Neolithic age. In Serbia, cereals are predominantly grown in its northern part between 44◦
and 45.5◦ N of the Pannonian Plain. One of the most economically important nematodes on wheat
is the cereal cyst nematode, Heterodera filipjevi. Cysts of H. filipjevi survive in soil for years and
shelter a large number of microorganisms. The aims of this study were to investigate the diversity of
mycobiota associated with the cereal cyst nematode H. filipjevi, to infer phylogenetic relationships of
the found mycobiota, and to explore the ecological connection between fungi and the field history,
including the potential of fungi in bioremediation and the production of novel bioactive compounds.
Cysts were isolated from soil samples with a Spears apparatus and collected on a 150-µm sieve. The
cysts were placed on potato dextrose agar, and maintained for two weeks at 27◦C. Following fungal
isolation and colony growing, the fungal DNA was extracted, the ITS region was amplified, and
PCR products were sequenced. The study showed that the isolated fungal species belong to diverse
phyla, including Ascomycota, Basidiomycota, and Mucoromycota. Ascomycota is represented by
the families Clavicipitaceae, Sarocladiaceae, Nectriaceae, and Phaeosphaeriaceae. Basidiomycota
is represented by the families Cerrenaceae, Polyporaceae, Phanerochaetaceae, and Meruliaceae,
and the order Cantharellales. The family Mortierellaceae represents Mucoromycota. The members
of Ascomycota and Basidiomycota both depict the field history. Ascomycota indicate the fungal
infection is of recent origin, while Basidiomycota point toward the preceding host plants, enabling
the plant field colonization history to be traced chronologically
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biology
T1  - Diversity of Mycobiota Associated with the Cereal Cyst Nematode Heterodera filipjevi Originating from Some Localities of the Pannonian Plain in Serbia
VL  - 10
IS  - 4
SP  - 283
DO  - 10.3390/biology10040283
ER  - 

@article{
author = "Oro, Violeta and Stanisavljević, Rade and Nikolić, Bogdan and Tabaković, Marijenka and Sečanski, Mile and Solveig, Tosi",
year = "2021",
abstract = "Cereals, particularly wheat, are staple food of the people from the Balkans, dating back
to the Neolithic age. In Serbia, cereals are predominantly grown in its northern part between 44◦
and 45.5◦ N of the Pannonian Plain. One of the most economically important nematodes on wheat
is the cereal cyst nematode, Heterodera filipjevi. Cysts of H. filipjevi survive in soil for years and
shelter a large number of microorganisms. The aims of this study were to investigate the diversity of
mycobiota associated with the cereal cyst nematode H. filipjevi, to infer phylogenetic relationships of
the found mycobiota, and to explore the ecological connection between fungi and the field history,
including the potential of fungi in bioremediation and the production of novel bioactive compounds.
Cysts were isolated from soil samples with a Spears apparatus and collected on a 150-µm sieve. The
cysts were placed on potato dextrose agar, and maintained for two weeks at 27◦C. Following fungal
isolation and colony growing, the fungal DNA was extracted, the ITS region was amplified, and
PCR products were sequenced. The study showed that the isolated fungal species belong to diverse
phyla, including Ascomycota, Basidiomycota, and Mucoromycota. Ascomycota is represented by
the families Clavicipitaceae, Sarocladiaceae, Nectriaceae, and Phaeosphaeriaceae. Basidiomycota
is represented by the families Cerrenaceae, Polyporaceae, Phanerochaetaceae, and Meruliaceae,
and the order Cantharellales. The family Mortierellaceae represents Mucoromycota. The members
of Ascomycota and Basidiomycota both depict the field history. Ascomycota indicate the fungal
infection is of recent origin, while Basidiomycota point toward the preceding host plants, enabling
the plant field colonization history to be traced chronologically",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biology",
title = "Diversity of Mycobiota Associated with the Cereal Cyst Nematode Heterodera filipjevi Originating from Some Localities of the Pannonian Plain in Serbia",
volume = "10",
number = "4",
pages = "283",
doi = "10.3390/biology10040283"
}

Oro, V., Stanisavljević, R., Nikolić, B., Tabaković, M., Sečanski, M.,& Solveig, T.. (2021). Diversity of Mycobiota Associated with the Cereal Cyst Nematode Heterodera filipjevi Originating from Some Localities of the Pannonian Plain in Serbia. in Biology
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 10(4), 283.
https://doi.org/10.3390/biology10040283

Oro V, Stanisavljević R, Nikolić B, Tabaković M, Sečanski M, Solveig T. Diversity of Mycobiota Associated with the Cereal Cyst Nematode Heterodera filipjevi Originating from Some Localities of the Pannonian Plain in Serbia. in Biology. 2021;10(4):283.
doi:10.3390/biology10040283 .

Oro, Violeta, Stanisavljević, Rade, Nikolić, Bogdan, Tabaković, Marijenka, Sečanski, Mile, Solveig, Tosi, "Diversity of Mycobiota Associated with the Cereal Cyst Nematode Heterodera filipjevi Originating from Some Localities of the Pannonian Plain in Serbia" in Biology, 10, no. 4 (2021):283,
https://doi.org/10.3390/biology10040283 . .