Ristić, Danijela

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orcid::0000-0002-3145-4158
  • Ristić, Danijela (46)
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Author's Bibliography

Uticaj hibrida kukuruza i primenjenih insekticida na napad Ostrinia nubilalis HBN.

Grčak, Dragan; Gošić-Dondo, Snežana; Grčak, Milosav; Ristić, Danijela; Kondić, Danijela; Hajder, Đurađ; Popović, Željko D.; Knezevic, Desimir

(Belgrade : Serbian Genetics Society, 2022-01)

TY  - JOUR
AU  - Grčak, Dragan
AU  - Gošić-Dondo, Snežana
AU  - Grčak, Milosav
AU  - Ristić, Danijela
AU  - Kondić, Danijela
AU  - Hajder, Đurađ
AU  - Popović, Željko D.
AU  - Knezevic, Desimir
PY  - 2022-01
UR  - http://rik.mrizp.rs/handle/123456789/973
AB  - Ostrinia nubilalis Hbn. je štetočina kukuruza koja u pojedinim godišnjim dobima može naneti
veliku štetu biljkama. Cilj ovog rada je proučavanje otpornosti odnosno podložnosti hibrida
napadu kukuruznog plamenca i efekata različitih doza insekticida i vremena primene u zaštiti od
ove štetočine na biljkama kukuruza, ali prvenstveno uticaj hibrida na intenzitet napada. Šest
hibrida kukuruza različitih grupa zrenja (ZP 427, ZP 434, ZP 555, ZP 600, ZP 606 i ZP 666)
korišćeno je za proučavanje dejstva napada Ostrinia nubilalis Hbn., na kontrolnu varijantu T1
bez primene insekticida i na varijantu tretiranu sa dva insekticida Fobos EC (bifentrin - 200 ml
ha-1) i kombinacijom Match 050 EC i Nurelle D (lufenuron 50 g l-1 i hlorpirifos 500 g l-1 +
cipermetrin 50 g l-1), 0.75 l ha-1) u dva termina (u prvoj generaciji za vreme leta insekata T2 i
T3 i u drugoj generaciji za vreme leta insekata T4 i T5) u tri ponavljanja. Rezultati su pokazali
najveći intenzitet Ostrinia nubilalis Hbn. napad i stepen oštećenja na kontrolnoj varijanti i
značajne razlike između tretmana za ispitivane parametre. Posmatrajući sve hibride i tretmane,
nije utvrđena statistička značajnost između šest ispitivanih hibrida kukuruza različitih grupa
zrelosti. U kontrolnoj varijanti najveći napad (oštećenje) je bio na biljkama kod hibrida ZP 666
(94,28%) i ZP 606 (93,90%), a među varijantama primenjenih insekticida najveća šteta je
konstatovana u tretmanu T5 sa primenjena kombinacija Match + Nurelle u hibridu ZP 555 i
iznosila je 92,02%. Na tretmanu T1-kontrolna varijanta bez insekticida, najmanji napad sa
Ostrinia nubilalis Hbn. je konstatovan kod hibrida ZP 434 (od 88,76%), a među varijantama
primenjenih insekticida najmanji napad je konstatovan kod varijante T2 sa primenjenim Fobos
kod prve generacije insekata u hibridu ZP 434 i iznosio je 77,12%. Napad Ostrinia nubilalis
Hbn. i varijacija prosečne mase klipa kukuruza varirala je u zavisnosti od hibrida kukuruza i
vremena primene insekticida.
AB  - Ostrinia nubilalis Hbn. is a corn pest that can do great damage to plants in some seasons.
The aim of this paper is to study the resistance / susceptibility of hybrids to the attack of
European corn borer and the effects of different doses of insecticides and time of
application in protection against pests on maize plants, but primarily the influence of
hybrids on attack intensity. The six maize hybrids of different maturation groups (ZP 427,
ZP 434, ZP 555, ZP 600, ZP 606 and ZP 666) used for study effect of attack of Ostrinia
nubillalis Hbn., on control variant T1 without application of insecticide and on variant
treated with two insecticides Fobos EC (bifentrin - 200 ml ha-1
) and combination of Match
050 EC and Nurelle D (lufenuron 50 g l
-1
and hlorpirifos 500 g l
-1 + cipermetrin 50 g l
-1
),
0.75 l ha-1
) in two terms (in the first generation of insect flying T2 and T3 and in the
second generation of insect flying T4 and T5) in three replication. The results showed the
highest intensity of Ostrinia nubilalis Hbn. attack and degree of damages on control
variant and significant differences between treatments for investigated parameters.
Observing all hybrids and treatments, there was no statistical significance between the six
examined maize hybrids of different maturity groups. In the control variant, the greatest
attack (damage) was in plants, namely in the hybrid ZP 666 (94.28%) and ZP 606 (93.90%) and among the variants of applied insecticides, the greatest damage was found
in the treatment T5 with the applied combination of Match + Nurelle in hybrid ZP 555
and was 92.02%. On treatment T1-control variant without insecticides, the smallest attack
with Ostrinia nubilalis Hbn. was found in the hybrid ZP 434 (from 88.76%) and among
the variants of applied insecticides, the smallest attack was found on variant T2 with
applied Phobos in the first generation of insect in hybrid ZP 434 and amounted to
77.12%. The attack of Ostrinia nubilalis Hbn. and variation of average mass of maize cob
varied in dependence of maze of hybrids and variant of insecticides application.
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Uticaj hibrida kukuruza i primenjenih insekticida na napad  Ostrinia nubilalis HBN.
T1  - Influence of maize hybrids and applied  insecticides on  Ostrinia nubilalis HBN. attack
VL  - 54
IS  - 1
SP  - 289
EP  - 306
DO  - 10.2298/gensr2201289g
ER  - 
@article{
author = "Grčak, Dragan and Gošić-Dondo, Snežana and Grčak, Milosav and Ristić, Danijela and Kondić, Danijela and Hajder, Đurađ and Popović, Željko D. and Knezevic, Desimir",
year = "2022-01",
abstract = "Ostrinia nubilalis Hbn. je štetočina kukuruza koja u pojedinim godišnjim dobima može naneti
veliku štetu biljkama. Cilj ovog rada je proučavanje otpornosti odnosno podložnosti hibrida
napadu kukuruznog plamenca i efekata različitih doza insekticida i vremena primene u zaštiti od
ove štetočine na biljkama kukuruza, ali prvenstveno uticaj hibrida na intenzitet napada. Šest
hibrida kukuruza različitih grupa zrenja (ZP 427, ZP 434, ZP 555, ZP 600, ZP 606 i ZP 666)
korišćeno je za proučavanje dejstva napada Ostrinia nubilalis Hbn., na kontrolnu varijantu T1
bez primene insekticida i na varijantu tretiranu sa dva insekticida Fobos EC (bifentrin - 200 ml
ha-1) i kombinacijom Match 050 EC i Nurelle D (lufenuron 50 g l-1 i hlorpirifos 500 g l-1 +
cipermetrin 50 g l-1), 0.75 l ha-1) u dva termina (u prvoj generaciji za vreme leta insekata T2 i
T3 i u drugoj generaciji za vreme leta insekata T4 i T5) u tri ponavljanja. Rezultati su pokazali
najveći intenzitet Ostrinia nubilalis Hbn. napad i stepen oštećenja na kontrolnoj varijanti i
značajne razlike između tretmana za ispitivane parametre. Posmatrajući sve hibride i tretmane,
nije utvrđena statistička značajnost između šest ispitivanih hibrida kukuruza različitih grupa
zrelosti. U kontrolnoj varijanti najveći napad (oštećenje) je bio na biljkama kod hibrida ZP 666
(94,28%) i ZP 606 (93,90%), a među varijantama primenjenih insekticida najveća šteta je
konstatovana u tretmanu T5 sa primenjena kombinacija Match + Nurelle u hibridu ZP 555 i
iznosila je 92,02%. Na tretmanu T1-kontrolna varijanta bez insekticida, najmanji napad sa
Ostrinia nubilalis Hbn. je konstatovan kod hibrida ZP 434 (od 88,76%), a među varijantama
primenjenih insekticida najmanji napad je konstatovan kod varijante T2 sa primenjenim Fobos
kod prve generacije insekata u hibridu ZP 434 i iznosio je 77,12%. Napad Ostrinia nubilalis
Hbn. i varijacija prosečne mase klipa kukuruza varirala je u zavisnosti od hibrida kukuruza i
vremena primene insekticida., Ostrinia nubilalis Hbn. is a corn pest that can do great damage to plants in some seasons.
The aim of this paper is to study the resistance / susceptibility of hybrids to the attack of
European corn borer and the effects of different doses of insecticides and time of
application in protection against pests on maize plants, but primarily the influence of
hybrids on attack intensity. The six maize hybrids of different maturation groups (ZP 427,
ZP 434, ZP 555, ZP 600, ZP 606 and ZP 666) used for study effect of attack of Ostrinia
nubillalis Hbn., on control variant T1 without application of insecticide and on variant
treated with two insecticides Fobos EC (bifentrin - 200 ml ha-1
) and combination of Match
050 EC and Nurelle D (lufenuron 50 g l
-1
and hlorpirifos 500 g l
-1 + cipermetrin 50 g l
-1
),
0.75 l ha-1
) in two terms (in the first generation of insect flying T2 and T3 and in the
second generation of insect flying T4 and T5) in three replication. The results showed the
highest intensity of Ostrinia nubilalis Hbn. attack and degree of damages on control
variant and significant differences between treatments for investigated parameters.
Observing all hybrids and treatments, there was no statistical significance between the six
examined maize hybrids of different maturity groups. In the control variant, the greatest
attack (damage) was in plants, namely in the hybrid ZP 666 (94.28%) and ZP 606 (93.90%) and among the variants of applied insecticides, the greatest damage was found
in the treatment T5 with the applied combination of Match + Nurelle in hybrid ZP 555
and was 92.02%. On treatment T1-control variant without insecticides, the smallest attack
with Ostrinia nubilalis Hbn. was found in the hybrid ZP 434 (from 88.76%) and among
the variants of applied insecticides, the smallest attack was found on variant T2 with
applied Phobos in the first generation of insect in hybrid ZP 434 and amounted to
77.12%. The attack of Ostrinia nubilalis Hbn. and variation of average mass of maize cob
varied in dependence of maze of hybrids and variant of insecticides application.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Uticaj hibrida kukuruza i primenjenih insekticida na napad  Ostrinia nubilalis HBN., Influence of maize hybrids and applied  insecticides on  Ostrinia nubilalis HBN. attack",
volume = "54",
number = "1",
pages = "289-306",
doi = "10.2298/gensr2201289g"
}
Grčak, D., Gošić-Dondo, S., Grčak, M., Ristić, D., Kondić, D., Hajder, Đ., Popović, Ž. D.,& Knezevic, D.. (2022-01). Uticaj hibrida kukuruza i primenjenih insekticida na napad  Ostrinia nubilalis HBN.. in Genetika
Belgrade : Serbian Genetics Society., 54(1), 289-306.
https://doi.org/10.2298/gensr2201289g
Grčak D, Gošić-Dondo S, Grčak M, Ristić D, Kondić D, Hajder Đ, Popović ŽD, Knezevic D. Uticaj hibrida kukuruza i primenjenih insekticida na napad  Ostrinia nubilalis HBN.. in Genetika. 2022;54(1):289-306.
doi:10.2298/gensr2201289g .
Grčak, Dragan, Gošić-Dondo, Snežana, Grčak, Milosav, Ristić, Danijela, Kondić, Danijela, Hajder, Đurađ, Popović, Željko D., Knezevic, Desimir, "Uticaj hibrida kukuruza i primenjenih insekticida na napad  Ostrinia nubilalis HBN." in Genetika, 54, no. 1 (2022-01):289-306,
https://doi.org/10.2298/gensr2201289g . .

Bioresponse of maize genotypes to european corn borer (ostrinia nubilalis hbn) attack and insecticide treatments

Gošić-Dondo, Snežana; Srdić, Jelena; Dragičević, Vesna; Popović, Željko D.; Filipovic, Milomir; Ristić, Danijela; Grčak, Dragan

(Belgrade : Maize Research Institute, Zemun Polje, 2022)

TY  - CONF
AU  - Gošić-Dondo, Snežana
AU  - Srdić, Jelena
AU  - Dragičević, Vesna
AU  - Popović, Željko D.
AU  - Filipovic, Milomir
AU  - Ristić, Danijela
AU  - Grčak, Dragan
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/967
UR  - http://rik.mrizp.rs/handle/123456789/978
AB  - During last years, the total losses from European corn borer (Ostrinia nubilalis Hbn.) can reach
a high threshold of harmfulness although the different agro-technical measures that are being
implemented. According to the sensitivity of hybrids, the damage may vary from only a few
percent in tolerant hybrids, up to 30-40% on moderately intolerant hybrids. The aim of this
study was to evaluate the variability and bioresponsiveness of four maize genotypes (ZP 427,
ZP 434, ZP 600 and ZP 666) to applied insecticides and bioinsecticides and the
interdependence of population dynamics. The research has been conducted in 2016 and 2018
in accordance with the EPPO method in field conditions in maize crops. Out of a total of 11.023
attacks in both years of research, in 2016. the largest number of plants (4.841) were with a
broken stalk above the ear and at the height of the ear and belonged to categories C3 to C8. In
2018. 3.672 plants were dominated by leaf damage and perforation in the stalk type H2.
Analyzing the effectiveness of the tested insecticides and bioinsecticides by application times,
an uniformity in the number of plants with recorded symptoms of damage was observed. Thus,
foliar treatment with chlorantraniliprole insecticide proved to be the most effective with the
least number of attacked plants with a high degree of damage. In contrast, bioinsecticide
treatment of plants, in addition to a large number of infested plants, was dominated by high
categories of damage that deviated from all other treatments as well as from the control. Within
the definition of the degree of tolerance of maize hybrids, the influence of treatment on changes
in secondary metabolites (phytins) in seeds was observed. It was noticed that there is a trend
of positive correlation of the amount of phytin in the seed (mg/g) with the attack of moths of
the first generation and the number of dissected larvae, as well as a trend of negative correlation
with the percentage of attacks of moths of the second generation. Correlation coefficients are
expressed: in the genotype ZP-600, while in the genotype ZP-666 the correlation is the lowest,
ie. irrelevant. The obtained results may be of great importance in future work aimed at the
selection of tolerant genotypes to attack corn flame, taking into account the role of phytin, as
an antioxidant and phosphorus depot in maize grain.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Bioresponse of maize genotypes to european corn borer (ostrinia nubilalis hbn) attack and insecticide treatments
SP  - 50
ER  - 
@conference{
author = "Gošić-Dondo, Snežana and Srdić, Jelena and Dragičević, Vesna and Popović, Željko D. and Filipovic, Milomir and Ristić, Danijela and Grčak, Dragan",
year = "2022",
abstract = "During last years, the total losses from European corn borer (Ostrinia nubilalis Hbn.) can reach
a high threshold of harmfulness although the different agro-technical measures that are being
implemented. According to the sensitivity of hybrids, the damage may vary from only a few
percent in tolerant hybrids, up to 30-40% on moderately intolerant hybrids. The aim of this
study was to evaluate the variability and bioresponsiveness of four maize genotypes (ZP 427,
ZP 434, ZP 600 and ZP 666) to applied insecticides and bioinsecticides and the
interdependence of population dynamics. The research has been conducted in 2016 and 2018
in accordance with the EPPO method in field conditions in maize crops. Out of a total of 11.023
attacks in both years of research, in 2016. the largest number of plants (4.841) were with a
broken stalk above the ear and at the height of the ear and belonged to categories C3 to C8. In
2018. 3.672 plants were dominated by leaf damage and perforation in the stalk type H2.
Analyzing the effectiveness of the tested insecticides and bioinsecticides by application times,
an uniformity in the number of plants with recorded symptoms of damage was observed. Thus,
foliar treatment with chlorantraniliprole insecticide proved to be the most effective with the
least number of attacked plants with a high degree of damage. In contrast, bioinsecticide
treatment of plants, in addition to a large number of infested plants, was dominated by high
categories of damage that deviated from all other treatments as well as from the control. Within
the definition of the degree of tolerance of maize hybrids, the influence of treatment on changes
in secondary metabolites (phytins) in seeds was observed. It was noticed that there is a trend
of positive correlation of the amount of phytin in the seed (mg/g) with the attack of moths of
the first generation and the number of dissected larvae, as well as a trend of negative correlation
with the percentage of attacks of moths of the second generation. Correlation coefficients are
expressed: in the genotype ZP-600, while in the genotype ZP-666 the correlation is the lowest,
ie. irrelevant. The obtained results may be of great importance in future work aimed at the
selection of tolerant genotypes to attack corn flame, taking into account the role of phytin, as
an antioxidant and phosphorus depot in maize grain.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Bioresponse of maize genotypes to european corn borer (ostrinia nubilalis hbn) attack and insecticide treatments",
pages = "50"
}
Gošić-Dondo, S., Srdić, J., Dragičević, V., Popović, Ž. D., Filipovic, M., Ristić, D.,& Grčak, D.. (2022). Bioresponse of maize genotypes to european corn borer (ostrinia nubilalis hbn) attack and insecticide treatments. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 50.
Gošić-Dondo S, Srdić J, Dragičević V, Popović ŽD, Filipovic M, Ristić D, Grčak D. Bioresponse of maize genotypes to european corn borer (ostrinia nubilalis hbn) attack and insecticide treatments. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;:50..
Gošić-Dondo, Snežana, Srdić, Jelena, Dragičević, Vesna, Popović, Željko D., Filipovic, Milomir, Ristić, Danijela, Grčak, Dragan, "Bioresponse of maize genotypes to european corn borer (ostrinia nubilalis hbn) attack and insecticide treatments" in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022):50.

Nutritional and cost effects of adapted quality protein maize on broiler feeding

Kostadinović, Marija; Ristić, Danijela; Vančetović, Jelena; Delić, Nenad; Ignjatović-Micić, Dragana

(Belgrade : Maize Research Institute, Zemun Polje, 2022)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Ignjatović-Micić, Dragana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/967
AB  - ZPQPM13 is a maize hybrid with improved protein quality, adapted to temperate climate growth conditions, developed by crossing a QPM version of ZPL5 commercial line (developed by marker assisted selection) and GS-6 (a QPM line with 50% tropical and 50% temperate germplasm). This hybrid has grain yield as commercial hybrids, over 90% of hard endosperm, contents of tryptophan and protein over 0.075% and 10%, respectively, and stability of tryptophan content over different locations and years. ZPQPM13 was tested for use in broiler feeds, with the aim to reduce utilization of costly synthetic lysine or soybean components. The feeding experiment on Ross 308 broilers was divided into two groups – control (fed with standard maize) and treatment (fed with QPM). In treatment group diets, ZPQPM13 was increased and soybean component decreased for 3%. Chemical analyses revealed higher contents of limiting essential amino acids in mercantile ZPQPM13 compared to standard maize – 0.44 vs. 0.25 % lysine, 0.077 vs. 0.070 % tryptophan and 0.39 vs. 0.27 % threonine. Duration of each feeding trial was 42 days, comprising three phases - starter (1-14 days), grower (15-35 days) and finisher (36-42 days). Feed intake (FI), body mass gain (BMG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. In treatment group, FI was reduced, while BMG and ADG were higher in starter phase but lower in grower and finisher phases. FCR was lower in all three phases in treatment group – 1.13 vs. 1.30, 1.25 vs. 1.33 and 1.28 vs. 1.45. Although a small drawback is that final weight was 3.4 % higher in control broilers, comparison of feed and meat prices show that financial benefits of using QPM are still significant compared to using standard maize in feeds, as soybean is four times more expensive than maize.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Nutritional and cost effects of adapted quality protein maize on broiler feeding
SP  - 70
ER  - 
@conference{
author = "Kostadinović, Marija and Ristić, Danijela and Vančetović, Jelena and Delić, Nenad and Ignjatović-Micić, Dragana",
year = "2022",
abstract = "ZPQPM13 is a maize hybrid with improved protein quality, adapted to temperate climate growth conditions, developed by crossing a QPM version of ZPL5 commercial line (developed by marker assisted selection) and GS-6 (a QPM line with 50% tropical and 50% temperate germplasm). This hybrid has grain yield as commercial hybrids, over 90% of hard endosperm, contents of tryptophan and protein over 0.075% and 10%, respectively, and stability of tryptophan content over different locations and years. ZPQPM13 was tested for use in broiler feeds, with the aim to reduce utilization of costly synthetic lysine or soybean components. The feeding experiment on Ross 308 broilers was divided into two groups – control (fed with standard maize) and treatment (fed with QPM). In treatment group diets, ZPQPM13 was increased and soybean component decreased for 3%. Chemical analyses revealed higher contents of limiting essential amino acids in mercantile ZPQPM13 compared to standard maize – 0.44 vs. 0.25 % lysine, 0.077 vs. 0.070 % tryptophan and 0.39 vs. 0.27 % threonine. Duration of each feeding trial was 42 days, comprising three phases - starter (1-14 days), grower (15-35 days) and finisher (36-42 days). Feed intake (FI), body mass gain (BMG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. In treatment group, FI was reduced, while BMG and ADG were higher in starter phase but lower in grower and finisher phases. FCR was lower in all three phases in treatment group – 1.13 vs. 1.30, 1.25 vs. 1.33 and 1.28 vs. 1.45. Although a small drawback is that final weight was 3.4 % higher in control broilers, comparison of feed and meat prices show that financial benefits of using QPM are still significant compared to using standard maize in feeds, as soybean is four times more expensive than maize.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Nutritional and cost effects of adapted quality protein maize on broiler feeding",
pages = "70"
}
Kostadinović, M., Ristić, D., Vančetović, J., Delić, N.,& Ignjatović-Micić, D.. (2022). Nutritional and cost effects of adapted quality protein maize on broiler feeding. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 70.
Kostadinović M, Ristić D, Vančetović J, Delić N, Ignjatović-Micić D. Nutritional and cost effects of adapted quality protein maize on broiler feeding. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;:70..
Kostadinović, Marija, Ristić, Danijela, Vančetović, Jelena, Delić, Nenad, Ignjatović-Micić, Dragana, "Nutritional and cost effects of adapted quality protein maize on broiler feeding" in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022):70.

Biochemical characterization of soybean genotypes for grain quality improvement

Ristić, Danijela; Perić, Vesna; Srebrić, Mirjana; Kostadinović, Marija; Kravić, Natalija; Kovinčić, Anika; Gošić-Dondo, Snežana

(Belgrade ; Maize Research Institute, Zemun Polje, 2021)

TY  - CONF
AU  - Ristić, Danijela
AU  - Perić, Vesna
AU  - Srebrić, Mirjana
AU  - Kostadinović, Marija
AU  - Kravić, Natalija
AU  - Kovinčić, Anika
AU  - Gošić-Dondo, Snežana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/966
AB  - Soybean is one of the most economically important grain crops. As the
source of plant protein and vegetable oil, it is widely used as food and
industrial crop. Quality of soybean oil is determined by fatty acid (FA)
composition. Biochemical variation of different varieties significantly
facilitates genetic screening of kernel traits and grain quality attributes, thus
leading to one of the most important breeding strategies - improvement of
the grain composition. The main objective of this work was to evaluate
whole kernel biochemical composition of eight soybean varieties and eight
lines from different breeding programs. Phytochemical characterization was
conducted for total protein and oil content, as well as for fatty acids content
(e.g. palmitic, stearic, oleic, linoleic and linolenic acid). According to
Kjedahl method, total protein content ranged from 37.01% to 39.91%. The
obtained results showed that the lowest and the highest values for oil content
were 19.00% and 21.34%, respectively. Gas Chromatography/Flame
Ionization Detector (GC-FID) was used for FA quantification and
determination. The most abundant FA was linoleic acid with a range from
47.45 to 55.7 g/100g, followed by oleic acid with detected rage from 22.59
to 29.07 g/100g of total FA content. Palmitic, linolenic and stearic FA
showed a significantly lower level varied from 9.57 to 11.51 g/100g, 5.42 to
7.18 g/100g and 3.73 to 5.86 g/100g, respectively. This variability observed
in kernel quality among soybean genotypes can provide valuable information
for future breeding.
PB  - Belgrade ;  Maize Research Institute, Zemun Polje
C3  - International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia
T1  - Biochemical characterization of soybean genotypes for grain quality improvement
SP  - 77
ER  - 
@conference{
author = "Ristić, Danijela and Perić, Vesna and Srebrić, Mirjana and Kostadinović, Marija and Kravić, Natalija and Kovinčić, Anika and Gošić-Dondo, Snežana",
year = "2021",
abstract = "Soybean is one of the most economically important grain crops. As the
source of plant protein and vegetable oil, it is widely used as food and
industrial crop. Quality of soybean oil is determined by fatty acid (FA)
composition. Biochemical variation of different varieties significantly
facilitates genetic screening of kernel traits and grain quality attributes, thus
leading to one of the most important breeding strategies - improvement of
the grain composition. The main objective of this work was to evaluate
whole kernel biochemical composition of eight soybean varieties and eight
lines from different breeding programs. Phytochemical characterization was
conducted for total protein and oil content, as well as for fatty acids content
(e.g. palmitic, stearic, oleic, linoleic and linolenic acid). According to
Kjedahl method, total protein content ranged from 37.01% to 39.91%. The
obtained results showed that the lowest and the highest values for oil content
were 19.00% and 21.34%, respectively. Gas Chromatography/Flame
Ionization Detector (GC-FID) was used for FA quantification and
determination. The most abundant FA was linoleic acid with a range from
47.45 to 55.7 g/100g, followed by oleic acid with detected rage from 22.59
to 29.07 g/100g of total FA content. Palmitic, linolenic and stearic FA
showed a significantly lower level varied from 9.57 to 11.51 g/100g, 5.42 to
7.18 g/100g and 3.73 to 5.86 g/100g, respectively. This variability observed
in kernel quality among soybean genotypes can provide valuable information
for future breeding.",
publisher = "Belgrade ;  Maize Research Institute, Zemun Polje",
journal = "International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia",
title = "Biochemical characterization of soybean genotypes for grain quality improvement",
pages = "77"
}
Ristić, D., Perić, V., Srebrić, M., Kostadinović, M., Kravić, N., Kovinčić, A.,& Gošić-Dondo, S.. (2021). Biochemical characterization of soybean genotypes for grain quality improvement. in International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia
Belgrade ;  Maize Research Institute, Zemun Polje., 77.
Ristić D, Perić V, Srebrić M, Kostadinović M, Kravić N, Kovinčić A, Gošić-Dondo S. Biochemical characterization of soybean genotypes for grain quality improvement. in International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia. 2021;:77..
Ristić, Danijela, Perić, Vesna, Srebrić, Mirjana, Kostadinović, Marija, Kravić, Natalija, Kovinčić, Anika, Gošić-Dondo, Snežana, "Biochemical characterization of soybean genotypes for grain quality improvement" in International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia (2021):77.

Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit

Kravić, Natalija; Babić, Vojka; Vukadinović, Jelena; Ristić, Danijela; Dragičević, Vesna; Mladenović-Drinić, Snežana; Anđelković, Violeta

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Vukadinović, Jelena
AU  - Ristić, Danijela
AU  - Dragičević, Vesna
AU  - Mladenović-Drinić, Snežana
AU  - Anđelković, Violeta
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/828
AB  - Plants reconfigure their metabolic pathways to cope with water deficit. The aim of this study was to determine the status of the physiological parameters and the content of phenolic acids in the upper most ear leaf of maize inbred lines contrasting in drought tolerance in terms of improved plant productivity e.g., increased grain yield. The experiment was conducted under irrigation and rain-fed conditions. In drought-tolerant lines, the effect of water deficit was reflected through a chlorophyll and nitrogen balance index increase followed by a flavonols index decrease. The opposite trend was noticed in drought susceptible inbreds, with the exception of the anthocyanins index. Moreover, in comparison to irrigation treatment, opposite trends in the correlations between grain yield and physiological parameters found under water deficit conditions indicated the activation of different metabolic pathways in defense against water deficit stress. Concerning phenolic acid content, water deficit caused the reduction of protocatechuic, caffeic, and sinapic acid in all inbreds evaluated. However, the highly pronounced increase of ferulic and especially cinnamic acid content under water deficit conditions indicated possible crucial role of these secondary metabolites in preventing the harmful effects of water deficit stress, which, in turn, might be useful in maize breeding selection for drought tolerance
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biology
T1  - Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit
VL  - 10
IS  - 8
SP  - 694
DO  - 10.3390/biology10080694
ER  - 
@article{
author = "Kravić, Natalija and Babić, Vojka and Vukadinović, Jelena and Ristić, Danijela and Dragičević, Vesna and Mladenović-Drinić, Snežana and Anđelković, Violeta",
year = "2021",
abstract = "Plants reconfigure their metabolic pathways to cope with water deficit. The aim of this study was to determine the status of the physiological parameters and the content of phenolic acids in the upper most ear leaf of maize inbred lines contrasting in drought tolerance in terms of improved plant productivity e.g., increased grain yield. The experiment was conducted under irrigation and rain-fed conditions. In drought-tolerant lines, the effect of water deficit was reflected through a chlorophyll and nitrogen balance index increase followed by a flavonols index decrease. The opposite trend was noticed in drought susceptible inbreds, with the exception of the anthocyanins index. Moreover, in comparison to irrigation treatment, opposite trends in the correlations between grain yield and physiological parameters found under water deficit conditions indicated the activation of different metabolic pathways in defense against water deficit stress. Concerning phenolic acid content, water deficit caused the reduction of protocatechuic, caffeic, and sinapic acid in all inbreds evaluated. However, the highly pronounced increase of ferulic and especially cinnamic acid content under water deficit conditions indicated possible crucial role of these secondary metabolites in preventing the harmful effects of water deficit stress, which, in turn, might be useful in maize breeding selection for drought tolerance",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biology",
title = "Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit",
volume = "10",
number = "8",
pages = "694",
doi = "10.3390/biology10080694"
}
Kravić, N., Babić, V., Vukadinović, J., Ristić, D., Dragičević, V., Mladenović-Drinić, S.,& Anđelković, V.. (2021). Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit. in Biology
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 694.
https://doi.org/10.3390/biology10080694
Kravić N, Babić V, Vukadinović J, Ristić D, Dragičević V, Mladenović-Drinić S, Anđelković V. Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit. in Biology. 2021;10(8):694.
doi:10.3390/biology10080694 .
Kravić, Natalija, Babić, Vojka, Vukadinović, Jelena, Ristić, Danijela, Dragičević, Vesna, Mladenović-Drinić, Snežana, Anđelković, Violeta, "Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit" in Biology, 10, no. 8 (2021):694,
https://doi.org/10.3390/biology10080694 . .
2
2

Genetic diversity of maize inbred lines assessed by SSR markers

Ristić, Danijela; Kostadinović, Marija; Kravić, Natalija; Kovinčić, Anika; Stevanović, Milan; Savić, Iva; Nikolić, Milica

(2020)

TY  - CONF
AU  - Ristić, Danijela
AU  - Kostadinović, Marija
AU  - Kravić, Natalija
AU  - Kovinčić, Anika
AU  - Stevanović, Milan
AU  - Savić, Iva
AU  - Nikolić, Milica
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/977
AB  - Morphological traits are the earliest used markers in germplasm characterization, but their application may be difficult due to the presence of recessive homozygous alleles and their low frequency. Simple Sequence Repeat (SSR) markers are widely used for estimation of genetic diversity within different species, due to their reproducibility, informativeness co-dominant and multi-allelic nature. They are also most effective for evaluation and selection of plant material, as well as assessment of genetic variability and relatedness of maize inbred lines. The aim of our work was to evaluate genetic diversity of maize inbred lines by SSR markers and compare results with their pedigre information. Seventeen polymorphic SSR markers were used to characterized 23 maize inbred lines that belong to diferent breeding programs. A total number of detected alleles was 78 and varied between two to nine, with an average of 4.6 alleles per marker. Based on presence or absence of alleles in each sample coefficient of similarity was calculated by Jaccard in NTSYSpc2 program package, version 2.1. The highest value (0.88) of genetic similarity was calculated between L21 and L22, while the lowest value (0.18) was found between inbred lines L3/L16 and L15/L20. Genetic similarity matrix was used to construct dendrogram by UPGMA (Unweighted Pair Group Method with Arithmetic Mean) method. Dendrogram analysis grouped maize inbred lines in one large cluster with 21 analyzed genotypes and one smaller cluster with two lines. Genetic heterogeneity betweean inbred lines detected by selected set of SSR markers, makes them a good choice for genetic diversity analysis and planning maize breeding programs.
C3  - 9. International symposium on agricultural sciences "AgroReS 2020", 24.09.2020., Banja Luka, B i H – Book of Abstracts
T1  - Genetic diversity of maize inbred lines assessed by SSR markers
SP  - 42
ER  - 
@conference{
author = "Ristić, Danijela and Kostadinović, Marija and Kravić, Natalija and Kovinčić, Anika and Stevanović, Milan and Savić, Iva and Nikolić, Milica",
year = "2020",
abstract = "Morphological traits are the earliest used markers in germplasm characterization, but their application may be difficult due to the presence of recessive homozygous alleles and their low frequency. Simple Sequence Repeat (SSR) markers are widely used for estimation of genetic diversity within different species, due to their reproducibility, informativeness co-dominant and multi-allelic nature. They are also most effective for evaluation and selection of plant material, as well as assessment of genetic variability and relatedness of maize inbred lines. The aim of our work was to evaluate genetic diversity of maize inbred lines by SSR markers and compare results with their pedigre information. Seventeen polymorphic SSR markers were used to characterized 23 maize inbred lines that belong to diferent breeding programs. A total number of detected alleles was 78 and varied between two to nine, with an average of 4.6 alleles per marker. Based on presence or absence of alleles in each sample coefficient of similarity was calculated by Jaccard in NTSYSpc2 program package, version 2.1. The highest value (0.88) of genetic similarity was calculated between L21 and L22, while the lowest value (0.18) was found between inbred lines L3/L16 and L15/L20. Genetic similarity matrix was used to construct dendrogram by UPGMA (Unweighted Pair Group Method with Arithmetic Mean) method. Dendrogram analysis grouped maize inbred lines in one large cluster with 21 analyzed genotypes and one smaller cluster with two lines. Genetic heterogeneity betweean inbred lines detected by selected set of SSR markers, makes them a good choice for genetic diversity analysis and planning maize breeding programs.",
journal = "9. International symposium on agricultural sciences "AgroReS 2020", 24.09.2020., Banja Luka, B i H – Book of Abstracts",
title = "Genetic diversity of maize inbred lines assessed by SSR markers",
pages = "42"
}
Ristić, D., Kostadinović, M., Kravić, N., Kovinčić, A., Stevanović, M., Savić, I.,& Nikolić, M.. (2020). Genetic diversity of maize inbred lines assessed by SSR markers. in 9. International symposium on agricultural sciences "AgroReS 2020", 24.09.2020., Banja Luka, B i H – Book of Abstracts, 42.
Ristić D, Kostadinović M, Kravić N, Kovinčić A, Stevanović M, Savić I, Nikolić M. Genetic diversity of maize inbred lines assessed by SSR markers. in 9. International symposium on agricultural sciences "AgroReS 2020", 24.09.2020., Banja Luka, B i H – Book of Abstracts. 2020;:42..
Ristić, Danijela, Kostadinović, Marija, Kravić, Natalija, Kovinčić, Anika, Stevanović, Milan, Savić, Iva, Nikolić, Milica, "Genetic diversity of maize inbred lines assessed by SSR markers" in 9. International symposium on agricultural sciences "AgroReS 2020", 24.09.2020., Banja Luka, B i H – Book of Abstracts (2020):42.

Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation

Kostadinović, Marija; Janjić, Jovana; Ristić, Danijela; Đorđević-Melnik, Olivera; Božinović, Sofija; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2020)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Janjić, Jovana
AU  - Ristić, Danijela
AU  - Đorđević-Melnik, Olivera
AU  - Božinović, Sofija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/962
AB  - This paper is a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions using the marker assisted selection. When SSR marker specific to the crtRB1 gene has been tested on parental lines, distinct polymorphism was observed between the donor and recurrent parents. The same marker has also been validated in BC1 generation when heterozygous individuals were clearly distinguishable from the homozygous dominants. The results presented herein refer to two-level selection procedure in BC2 generation. First, BC2 plants were analyzed with crtRB1-specific molecular marker to separate heterozygotes from dominant homozygotes. Percentage of heterozygous plants was approximately 50%, which was in accordance with the expected Mendelian ratio of 1:1. Second, the selected heterozygotes were screened with 30 polymorphic SSR markers distributed throughout the maize genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The RPG values among three analyzed parental lines and their respective progenies ranged from 85-99%. For each line separately, RPG values were: 86-97% (RP1), 90-95% (RP2) and 85-99% (RP3). Plants with RPG above 95% were selfed to produce BC2F2 generation in which homozygous recessive individuals would be identified. Those genotypes will be subjected to biochemical and phenotypic evaluation to confirm their nutritional and agronomical superiority. Finally, these β-carotene enriched lines, as well as the resulting improved hybrids, can be used in the biofortification programs.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings
T1  - Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation
SP  - 37
EP  - 44
ER  - 
@conference{
author = "Kostadinović, Marija and Janjić, Jovana and Ristić, Danijela and Đorđević-Melnik, Olivera and Božinović, Sofija and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2020",
abstract = "This paper is a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions using the marker assisted selection. When SSR marker specific to the crtRB1 gene has been tested on parental lines, distinct polymorphism was observed between the donor and recurrent parents. The same marker has also been validated in BC1 generation when heterozygous individuals were clearly distinguishable from the homozygous dominants. The results presented herein refer to two-level selection procedure in BC2 generation. First, BC2 plants were analyzed with crtRB1-specific molecular marker to separate heterozygotes from dominant homozygotes. Percentage of heterozygous plants was approximately 50%, which was in accordance with the expected Mendelian ratio of 1:1. Second, the selected heterozygotes were screened with 30 polymorphic SSR markers distributed throughout the maize genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The RPG values among three analyzed parental lines and their respective progenies ranged from 85-99%. For each line separately, RPG values were: 86-97% (RP1), 90-95% (RP2) and 85-99% (RP3). Plants with RPG above 95% were selfed to produce BC2F2 generation in which homozygous recessive individuals would be identified. Those genotypes will be subjected to biochemical and phenotypic evaluation to confirm their nutritional and agronomical superiority. Finally, these β-carotene enriched lines, as well as the resulting improved hybrids, can be used in the biofortification programs.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings",
title = "Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation",
pages = "37-44"
}
Kostadinović, M., Janjić, J., Ristić, D., Đorđević-Melnik, O., Božinović, S., Ignjatović-Micić, D.,& Vančetović, J.. (2020). Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation. in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 37-44.
Kostadinović M, Janjić J, Ristić D, Đorđević-Melnik O, Božinović S, Ignjatović-Micić D, Vančetović J. Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation. in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings. 2020;:37-44..
Kostadinović, Marija, Janjić, Jovana, Ristić, Danijela, Đorđević-Melnik, Olivera, Božinović, Sofija, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation" in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings (2020):37-44.

Morphological and physiological response of maize seedlings to chilling stress

Nikolić, Ana; Kravić, Natalija; Ristić, Danijela; Anđelković, Violeta; Marković, Ksenija; Vančetović, Jelena; Ignjatović-Micić, Dragana

(Beograd : Društavo genetičara Srbije, 2020)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kravić, Natalija
AU  - Ristić, Danijela
AU  - Anđelković, Violeta
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/814
AB  - Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing.
AB  - Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve
PB  - Beograd : Društavo genetičara Srbije
T2  - Genetika
T1  - Morphological and physiological response of maize seedlings to chilling stress
VL  - 52
IS  - 2
SP  - 689
EP  - 698
DO  - 10.2298/GENSR2002689N
ER  - 
@article{
author = "Nikolić, Ana and Kravić, Natalija and Ristić, Danijela and Anđelković, Violeta and Marković, Ksenija and Vančetović, Jelena and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing., Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve",
publisher = "Beograd : Društavo genetičara Srbije",
journal = "Genetika",
title = "Morphological and physiological response of maize seedlings to chilling stress",
volume = "52",
number = "2",
pages = "689-698",
doi = "10.2298/GENSR2002689N"
}
Nikolić, A., Kravić, N., Ristić, D., Anđelković, V., Marković, K., Vančetović, J.,& Ignjatović-Micić, D.. (2020). Morphological and physiological response of maize seedlings to chilling stress. in Genetika
Beograd : Društavo genetičara Srbije., 52(2), 689-698.
https://doi.org/10.2298/GENSR2002689N
Nikolić A, Kravić N, Ristić D, Anđelković V, Marković K, Vančetović J, Ignjatović-Micić D. Morphological and physiological response of maize seedlings to chilling stress. in Genetika. 2020;52(2):689-698.
doi:10.2298/GENSR2002689N .
Nikolić, Ana, Kravić, Natalija, Ristić, Danijela, Anđelković, Violeta, Marković, Ksenija, Vančetović, Jelena, Ignjatović-Micić, Dragana, "Morphological and physiological response of maize seedlings to chilling stress" in Genetika, 52, no. 2 (2020):689-698,
https://doi.org/10.2298/GENSR2002689N . .
3
2

Genetic diversity among soybean genotypes assessed by molecular markers

Ristić, Danijela; Filipovski, Filip; Perić, Vesna; Kostadinović, Marija; Gošić-Dondo, Snežana; Mladenović Drinić, Snežana

(London : SciTechnol, 2019)

TY  - CONF
AU  - Ristić, Danijela
AU  - Filipovski, Filip
AU  - Perić, Vesna
AU  - Kostadinović, Marija
AU  - Gošić-Dondo, Snežana
AU  - Mladenović Drinić, Snežana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/980
AB  - Soybean (Glycine max (L.) Merr.) is one of the oldest crop in the world, with a history of growing over 4000 years. At the same time it is the most important legume in the global world production. Simple Sequence Repeat (SSR) markers are widely used for estimation of genetic diversity within different species, due to their reproducibility, co-dominant and multi-allelic nature. Molecular characterization among soybean genotypes was done with 36 SSR markers, with at least one primer from each linkage group, to determine the genetic diversity. Twenty soybean accessions and varieties obtained from Maize Reasearch Institute „Zemun Polje“ were analyzed. A total number of detected alleles was 116 and number of alleles varied between two to six, with an average of 3.22 alleles per marker. Thirteen unique alleles were obtained from eleven SSR loci in nine genotypes. Molecular analysis revealed a high polymorphism of SSR loci. Pairwise coefficients of genetic similarity calculated by Dice ranged from 0.14 to 0.74. Genetic similarity matrix was used to construct dendrogram by UPGMA (Unweighted Pair Group Method with Arithmetic Mean) method in NTSYSpc2 program package. This clustering method distinguished 20 soybean genotypes in two subclusters and one branch.
PB  - London : SciTechnol
C3  - Journal of plant physiology & pathology
T1  - Genetic diversity among soybean genotypes assessed by molecular markers
VL  - 07
SP  - 37
ER  - 
@conference{
author = "Ristić, Danijela and Filipovski, Filip and Perić, Vesna and Kostadinović, Marija and Gošić-Dondo, Snežana and Mladenović Drinić, Snežana",
year = "2019",
abstract = "Soybean (Glycine max (L.) Merr.) is one of the oldest crop in the world, with a history of growing over 4000 years. At the same time it is the most important legume in the global world production. Simple Sequence Repeat (SSR) markers are widely used for estimation of genetic diversity within different species, due to their reproducibility, co-dominant and multi-allelic nature. Molecular characterization among soybean genotypes was done with 36 SSR markers, with at least one primer from each linkage group, to determine the genetic diversity. Twenty soybean accessions and varieties obtained from Maize Reasearch Institute „Zemun Polje“ were analyzed. A total number of detected alleles was 116 and number of alleles varied between two to six, with an average of 3.22 alleles per marker. Thirteen unique alleles were obtained from eleven SSR loci in nine genotypes. Molecular analysis revealed a high polymorphism of SSR loci. Pairwise coefficients of genetic similarity calculated by Dice ranged from 0.14 to 0.74. Genetic similarity matrix was used to construct dendrogram by UPGMA (Unweighted Pair Group Method with Arithmetic Mean) method in NTSYSpc2 program package. This clustering method distinguished 20 soybean genotypes in two subclusters and one branch.",
publisher = "London : SciTechnol",
journal = "Journal of plant physiology & pathology",
title = "Genetic diversity among soybean genotypes assessed by molecular markers",
volume = "07",
pages = "37"
}
Ristić, D., Filipovski, F., Perić, V., Kostadinović, M., Gošić-Dondo, S.,& Mladenović Drinić, S.. (2019). Genetic diversity among soybean genotypes assessed by molecular markers. in Journal of plant physiology & pathology
London : SciTechnol., 07, 37.
Ristić D, Filipovski F, Perić V, Kostadinović M, Gošić-Dondo S, Mladenović Drinić S. Genetic diversity among soybean genotypes assessed by molecular markers. in Journal of plant physiology & pathology. 2019;07:37..
Ristić, Danijela, Filipovski, Filip, Perić, Vesna, Kostadinović, Marija, Gošić-Dondo, Snežana, Mladenović Drinić, Snežana, "Genetic diversity among soybean genotypes assessed by molecular markers" in Journal of plant physiology & pathology, 07 (2019):37.

Molecular characterisation of maize hybrids

Ristić, Danijela; Kostadinović, Marija; Kravić, Natalija; Kovinčić, Anika; Obradović, Ana; Stevanović, Milan; Pavlov, Jovan

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2019)

TY  - CONF
AU  - Ristić, Danijela
AU  - Kostadinović, Marija
AU  - Kravić, Natalija
AU  - Kovinčić, Anika
AU  - Obradović, Ana
AU  - Stevanović, Milan
AU  - Pavlov, Jovan
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/979
AB  - Despite the huge diversity of maize germplasm, modern maize breeding programme and
agricultural practices decrease the diversity of modern hybrids. Genetic characterization of
maize hybrids allows knowledge of the genetic relationship among them, thus preventing the
risk of increasing uniformity. Because of their high reproducibility, informativeness and easey
application of microsatelites are the most frequently used molecular markers in maize genetic
diversity studies. The aim of our work was to evaluate genetic diversity of maize hybrids by
SSR markers and compare results with their pedigre information. Sixteen polymorphic SSR
(Simple Sequence Repeats) markers were used to characterize 14 maize (Zea mays L.) hybrids
belonging to different breeding programs and FAO groups (from 300 to 800). A total of 53
alleles were found, ranging from two to four alleles. Genetic similarities were calculated in
NTSYSpc2 program package using Jaccard’s coefficient based on binary data (presence or
absence of alleles). The highest value of genetic similarity was 0.80 between H1 and H2,
while the lowest value (0.26) was found between H12 and H13. Cluster analysis was done by
unweighted pair group method (UPGMA) on the basis of genetic similarity matrix.
Dendrogram analysis grouped maize hybrids in one cluster (most of the analyzed genotypes),
one smaller cluster and one branch. The results revealed genetic heterogeneity between analyzed maize hybrids.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina
T1  - Molecular characterisation of maize hybrids
SP  - 211
EP  - 215
ER  - 
@conference{
author = "Ristić, Danijela and Kostadinović, Marija and Kravić, Natalija and Kovinčić, Anika and Obradović, Ana and Stevanović, Milan and Pavlov, Jovan",
year = "2019",
abstract = "Despite the huge diversity of maize germplasm, modern maize breeding programme and
agricultural practices decrease the diversity of modern hybrids. Genetic characterization of
maize hybrids allows knowledge of the genetic relationship among them, thus preventing the
risk of increasing uniformity. Because of their high reproducibility, informativeness and easey
application of microsatelites are the most frequently used molecular markers in maize genetic
diversity studies. The aim of our work was to evaluate genetic diversity of maize hybrids by
SSR markers and compare results with their pedigre information. Sixteen polymorphic SSR
(Simple Sequence Repeats) markers were used to characterize 14 maize (Zea mays L.) hybrids
belonging to different breeding programs and FAO groups (from 300 to 800). A total of 53
alleles were found, ranging from two to four alleles. Genetic similarities were calculated in
NTSYSpc2 program package using Jaccard’s coefficient based on binary data (presence or
absence of alleles). The highest value of genetic similarity was 0.80 between H1 and H2,
while the lowest value (0.26) was found between H12 and H13. Cluster analysis was done by
unweighted pair group method (UPGMA) on the basis of genetic similarity matrix.
Dendrogram analysis grouped maize hybrids in one cluster (most of the analyzed genotypes),
one smaller cluster and one branch. The results revealed genetic heterogeneity between analyzed maize hybrids.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina",
title = "Molecular characterisation of maize hybrids",
pages = "211-215"
}
Ristić, D., Kostadinović, M., Kravić, N., Kovinčić, A., Obradović, A., Stevanović, M.,& Pavlov, J.. (2019). Molecular characterisation of maize hybrids. in 10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 211-215.
Ristić D, Kostadinović M, Kravić N, Kovinčić A, Obradović A, Stevanović M, Pavlov J. Molecular characterisation of maize hybrids. in 10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina. 2019;:211-215..
Ristić, Danijela, Kostadinović, Marija, Kravić, Natalija, Kovinčić, Anika, Obradović, Ana, Stevanović, Milan, Pavlov, Jovan, "Molecular characterisation of maize hybrids" in 10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina (2019):211-215.

Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Ristić, Danijela; Mladenović-Drinić, Snežana

(London : Insight Medical Publishing, 2019)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/970
AB  - Worldwide, modern grain breeding programs widely implement marker assisted selection (MAS). Molecular markers are being used in two ways: to control the target gene (foreground selection) and to accelerate the reconstruction of the recurrent parent genotype (background selection). Marker analysis allow breeders to discard plants without allels of interest prior to pollination, reducing the size of breeding population. Marker assisted selection for the qualitative traits, which are regulated by the action of a single or several genes and are clearly phenotypically defined, achieved the best results. The improvement of β-carotene content using crtRB1-specific molecular marker is one of the successful examples of MAS. The introgressed inbreds possessing favourable allele of crtRB1 and improved hybrids with increased β-carotene can be used in the biofortification program.
The results presented herein are a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions. The utility of SSR marker specific to the crtRB1 gene in foreground selection has been already tested on parental lines and distinct marker polymorphism was observed between the donor and recurrent parents. The objective of this study was to validate the ability of the same SSR marker to detect homozygous dominant and heterozygous plants separately in BC1 generation. Heterozygous individuals were clearly distinguishable, therefore they will be selected and backcrossed with the recurrent parent to produce BC2 generation. Percentage of heterozygous plants identified in BC1 generation was approximately 50%, which is in accordance with the expected Mendelian ratio of 1 O2O2 : 1 O2o2. The use of the crtRB1-specific marker will be extended in the next generation of MAS for β-carotene enriched lines for growing in temperate regions.
PB  - London : Insight Medical Publishing
C3  - 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research
T1  - Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation
VL  - 09
SP  - 27
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Mladenović-Drinić, Snežana",
year = "2019",
abstract = "Worldwide, modern grain breeding programs widely implement marker assisted selection (MAS). Molecular markers are being used in two ways: to control the target gene (foreground selection) and to accelerate the reconstruction of the recurrent parent genotype (background selection). Marker analysis allow breeders to discard plants without allels of interest prior to pollination, reducing the size of breeding population. Marker assisted selection for the qualitative traits, which are regulated by the action of a single or several genes and are clearly phenotypically defined, achieved the best results. The improvement of β-carotene content using crtRB1-specific molecular marker is one of the successful examples of MAS. The introgressed inbreds possessing favourable allele of crtRB1 and improved hybrids with increased β-carotene can be used in the biofortification program.
The results presented herein are a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions. The utility of SSR marker specific to the crtRB1 gene in foreground selection has been already tested on parental lines and distinct marker polymorphism was observed between the donor and recurrent parents. The objective of this study was to validate the ability of the same SSR marker to detect homozygous dominant and heterozygous plants separately in BC1 generation. Heterozygous individuals were clearly distinguishable, therefore they will be selected and backcrossed with the recurrent parent to produce BC2 generation. Percentage of heterozygous plants identified in BC1 generation was approximately 50%, which is in accordance with the expected Mendelian ratio of 1 O2O2 : 1 O2o2. The use of the crtRB1-specific marker will be extended in the next generation of MAS for β-carotene enriched lines for growing in temperate regions.",
publisher = "London : Insight Medical Publishing",
journal = "5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research",
title = "Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation",
volume = "09",
pages = "27"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D.,& Mladenović-Drinić, S.. (2019). Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation. in 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research
London : Insight Medical Publishing., 09, 27.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Mladenović-Drinić S. Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation. in 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research. 2019;09:27..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Mladenović-Drinić, Snežana, "Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation" in 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research, 09 (2019):27.

The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version

Kostadinović, Marija; Ristić, Danijela; Kovinčić, Anika; Simić, Marijana; Perić, Vesna; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Belgrade : Serbian Genetic Society, 2019)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Kovinčić, Anika
AU  - Simić, Marijana
AU  - Perić, Vesna
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/963
AB  - Quality Protein Maize (QPM) is nutritionally and agronomically improved maize. In order to shorten the period required for development of QPM hybrids through the conventional method of backcrossing, marker assisted selection (MAS) is being used. After successful conversion of one commercial maize inbred line to its QPM counterpart for growing in temperate climate, this breeding program was continued at Maize Research Institute (MRI) with larger number of maize lines. Four commercial MRI inbreds, chosen for marker assisted introgression of the quality protein trait, and their BC2 progenies were subjected to two-level selection procedure. First, BC2 plants were analyzed with opaque2 (o2) specific molecular markers to identify heterozygotes. Second, the selected heterozygotes were screened with SSR markers distributed throughout the genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The specific markers identified 100 out of 192 plants (52%) as heterozygous. Genetic similarity values between parental lines and their BC2 heterozygous progenies were in the range from 0.77 to 0.99 (77-99% RPG). The highest proportion of RPG was found in L1 (93-99%) and the lowest in L3 progenies (77-89%). Average values for the RPG content ranged from 83.9 to 95.8%. Progenies with RPG above 95% were selfed to produce BC2F2 plants which will be subjected to foreground selection. This time selection will be focused on homozygous recessive individuals, given that the presence of opaque2 gene in the homozygous recessive state is the aim of the QPM selection. Finally, those o2o2 genotypes will be screened for biochemical and phenotypic traits to confirm their nutritional and agronomical superiority.
PB  - Belgrade : Serbian Genetic Society
C3  - 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings
T1  - The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version
SP  - 23
EP  - 30
ER  - 
@conference{
author = "Kostadinović, Marija and Ristić, Danijela and Kovinčić, Anika and Simić, Marijana and Perić, Vesna and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Quality Protein Maize (QPM) is nutritionally and agronomically improved maize. In order to shorten the period required for development of QPM hybrids through the conventional method of backcrossing, marker assisted selection (MAS) is being used. After successful conversion of one commercial maize inbred line to its QPM counterpart for growing in temperate climate, this breeding program was continued at Maize Research Institute (MRI) with larger number of maize lines. Four commercial MRI inbreds, chosen for marker assisted introgression of the quality protein trait, and their BC2 progenies were subjected to two-level selection procedure. First, BC2 plants were analyzed with opaque2 (o2) specific molecular markers to identify heterozygotes. Second, the selected heterozygotes were screened with SSR markers distributed throughout the genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The specific markers identified 100 out of 192 plants (52%) as heterozygous. Genetic similarity values between parental lines and their BC2 heterozygous progenies were in the range from 0.77 to 0.99 (77-99% RPG). The highest proportion of RPG was found in L1 (93-99%) and the lowest in L3 progenies (77-89%). Average values for the RPG content ranged from 83.9 to 95.8%. Progenies with RPG above 95% were selfed to produce BC2F2 plants which will be subjected to foreground selection. This time selection will be focused on homozygous recessive individuals, given that the presence of opaque2 gene in the homozygous recessive state is the aim of the QPM selection. Finally, those o2o2 genotypes will be screened for biochemical and phenotypic traits to confirm their nutritional and agronomical superiority.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings",
title = "The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version",
pages = "23-30"
}
Kostadinović, M., Ristić, D., Kovinčić, A., Simić, M., Perić, V., Ignjatović-Micić, D.,& Vančetović, J.. (2019). The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version. in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings
Belgrade : Serbian Genetic Society., 23-30.
Kostadinović M, Ristić D, Kovinčić A, Simić M, Perić V, Ignjatović-Micić D, Vančetović J. The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version. in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings. 2019;:23-30..
Kostadinović, Marija, Ristić, Danijela, Kovinčić, Anika, Simić, Marijana, Perić, Vesna, Ignjatović-Micić, Dragana, Vančetović, Jelena, "The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version" in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings (2019):23-30.

Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Ristić, Danijela; Obradović, Ana; Đorđević-Melnik, Olivera; Kovinčić, Anika

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2019)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Obradović, Ana
AU  - Đorđević-Melnik, Olivera
AU  - Kovinčić, Anika
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/964
AB  - Marker assisted selection (MAS) significantly increases efficiency of conventional breeding. Molecular markers are utilized as selection markers for target genes (foreground selection) and also for identification of the genotypes (progenies) with the highest proportion of recurrent parent’s genome (background selection). Maize Research Institute "Zemun Polje" has a breeding program with the aim to create lines with incorporated incompatibility dominant gene Gametophytic Factor 1-S (Gа1-S), using the integrated conventional and molecular breeding approach. Ga1-S is the most described gene belonging to the group of genes specific to the pollen development, germination and pollen tube growth. The Ga1-S system is the most commonly used to prevent the pollination of sweetcorn, popcorn and white kerneld hybrids by standard maize. The objectives of this study were identification of gene-specific molecular marker for foreground selection, as well as the set of SSR markers polymorphic between parental lines to be used in background selection. Genetic variability between two donor and three recurrent parental inbred lines was analyzed with 42 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 83, average being 2.77. The genetic similarity values calculated on Dice coefficient ranged from 0.47 to 0.71. Among 12 gene-specific markers tested on parental lines, two showed distinct polymorphism for Ga1-S. These markers will be used as foreground selection markers for the incorporation of Gа1-S into our inbred lines which will be used for the creation of white kernel hybrids.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings
T1  - Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines
SP  - 91
EP  - 95
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Obradović, Ana and Đorđević-Melnik, Olivera and Kovinčić, Anika",
year = "2019",
abstract = "Marker assisted selection (MAS) significantly increases efficiency of conventional breeding. Molecular markers are utilized as selection markers for target genes (foreground selection) and also for identification of the genotypes (progenies) with the highest proportion of recurrent parent’s genome (background selection). Maize Research Institute "Zemun Polje" has a breeding program with the aim to create lines with incorporated incompatibility dominant gene Gametophytic Factor 1-S (Gа1-S), using the integrated conventional and molecular breeding approach. Ga1-S is the most described gene belonging to the group of genes specific to the pollen development, germination and pollen tube growth. The Ga1-S system is the most commonly used to prevent the pollination of sweetcorn, popcorn and white kerneld hybrids by standard maize. The objectives of this study were identification of gene-specific molecular marker for foreground selection, as well as the set of SSR markers polymorphic between parental lines to be used in background selection. Genetic variability between two donor and three recurrent parental inbred lines was analyzed with 42 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 83, average being 2.77. The genetic similarity values calculated on Dice coefficient ranged from 0.47 to 0.71. Among 12 gene-specific markers tested on parental lines, two showed distinct polymorphism for Ga1-S. These markers will be used as foreground selection markers for the incorporation of Gа1-S into our inbred lines which will be used for the creation of white kernel hybrids.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings",
title = "Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines",
pages = "91-95"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D., Obradović, A., Đorđević-Melnik, O.,& Kovinčić, A.. (2019). Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines. in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 91-95.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Obradović A, Đorđević-Melnik O, Kovinčić A. Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines. in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings. 2019;:91-95..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Obradović, Ana, Đorđević-Melnik, Olivera, Kovinčić, Anika, "Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines" in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings (2019):91-95.

Marker assisted backcross breeding in Maize Research Institute Zemun Polje

Kostadinović, Marija; Nikolić, Ana; Ristić, Danijela; Božinović, Sofija; Đorđević-Melnik, Olivera; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2019)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/743
AB  - Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije.
AB  - The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Marker assisted backcross breeding in Maize Research Institute Zemun Polje
T1  - Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje
VL  - 25
IS  - 1
SP  - 41
EP  - 47
DO  - 10.5937/SelSem1901041K
UR  - conv_2003
ER  - 
@article{
author = "Kostadinović, Marija and Nikolić, Ana and Ristić, Danijela and Božinović, Sofija and Đorđević-Melnik, Olivera and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije., The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Marker assisted backcross breeding in Maize Research Institute Zemun Polje, Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje",
volume = "25",
number = "1",
pages = "41-47",
doi = "10.5937/SelSem1901041K",
url = "conv_2003"
}
Kostadinović, M., Nikolić, A., Ristić, D., Božinović, S., Đorđević-Melnik, O., Ignjatović-Micić, D.,& Vančetović, J.. (2019). Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 25(1), 41-47.
https://doi.org/10.5937/SelSem1901041K
conv_2003
Kostadinović M, Nikolić A, Ristić D, Božinović S, Đorđević-Melnik O, Ignjatović-Micić D, Vančetović J. Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo. 2019;25(1):41-47.
doi:10.5937/SelSem1901041K
conv_2003 .
Kostadinović, Marija, Nikolić, Ana, Ristić, Danijela, Božinović, Sofija, Đorđević-Melnik, Olivera, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted backcross breeding in Maize Research Institute Zemun Polje" in Selekcija i semenarstvo, 25, no. 1 (2019):41-47,
https://doi.org/10.5937/SelSem1901041K .,
conv_2003 .
3

Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain

Popović, Vladan; Lučić, Aleksandar; Rakonjac, Ljubinko; Milovanović, Jelena; Mladenović-Drinić, Snežana; Ristić, Danijela

(Beograd : Društvo genetičara Srbije, 2019)

TY  - JOUR
AU  - Popović, Vladan
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Milovanović, Jelena
AU  - Mladenović-Drinić, Snežana
AU  - Ristić, Danijela
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/799
AB  - The process of plant breeding and conservation of gene pool among other things depends on the knowledge of the level of genetic differentiation. The aim of research in this paper was to determine the genetic differentiation of silver fir (Abies albaMill.) populations of regular  type  and  atypical  genotypes  with  pyramidal  crown  that  can  be  found  on  Javor mountain, at  the  site Ogorijevac. The  genetic  differentiation of silver fir  was determined using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave 29 alleles, while  the  average  number  of  alleles  was  3.2.  The  primer  NFH15  gave  the  smallest number of alleles (two), while the primer SF78 gave the greatest number of alleles (five). Dice  coefficient  of  the  genetic  similarity  was  used  to  obtain  a  dendrogram  by  UPMGA analysis  using  NTSYSpc  statistical  program.  The  genetic  similarity  recorded  among  the individuals P1 and P2 was the largest (0.89), while the populations VI and individual P2 showed the lowest similarity (0.61).Based on the cluster analysis it canbe concluded that the  studied populations and genotypes of silver  fir  with different types of crown are  clearly differentiated. The  basic insight  into  the  level  of  the  genetic  diversity  of  the  natural  populations  of  silver  fir  with the  various types of crown has been provided using selected SSR markers. The obtained results can be used for creating further strategy for the conservation of the available gene pool and the regeneration of silver fir forests in Serbia.
AB  - Proces oplemenjivanja biljaka i konzervacije genofonda između ostalog, zavisi i od poznavanja stepena  genetičke  diferencijacije.  Cilj  istraživanja  u  ovom  radu  bio  je  da  se  utvrdi  genetička diferencijacija   populacija   jele   (Abies   alba Mill.)normalanog  tipa  i  atipičnih  genotipova piramidalanog tipa krošnje, koje se javljaju na planini Javor, na lokalitetu Ogorijevac. Genetička sličnost odnosno različitost jele određena je upotrebom SSR (Simple Sequence Repeat) markera. Devet SSR pari prajmera dalo je ukupno 29 alela, dok je prosečan broj alela bio 3,2. Prajmer NFH15 dao je najmanji broj alela (dva), dok je prajmer SF78 bio sa najvećim brojem alela (pet). Najviša vrednost koeficijenta genetičke sličnosti utvrđena je između genotipova P1 i P2 (0,89), dok je najniža vrednost koeficijenta genetičke sličnosti utvrđena između populacije VI i genotipa P2 (0,61).Koeficijenti  genetičke  sličnosti  po  Dice,  upotrebljeni  su  za  dobijanje  dendrograma  pomoću UPMGA  analize,  koristeći  NTSYSpc  statistički  program.  Na  osnovu  klaster  analize  može  se zaključiti  da  su  istraživane  populacije  i  genotipovi  jele  sa  različitim  tipom  krošnje  jasno izdiferencirani.  Upotrebom  izabranih  SSR  markera  dat  je  osnovni  uvid  u  nivo  genetičke raznovrsnosti prirodnih populacija jele različitog tipa krošnje. Dobijeni rezultati mogu poslužiti u budućoj strategiji na konzervaciji raspoloživog genofonda i obnavljanju jelovih šuma u Srbiji.
PB  - Beograd : Društvo genetičara Srbije
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain
T1  - Procena genetičke diferencijacije jele (Abies alba Mill.) sa Javora pomoću SSR markera
VL  - 51
IS  - 3
SP  - 1103
EP  - 1112
DO  - 10.2298/GENSR1903103P
ER  - 
@article{
author = "Popović, Vladan and Lučić, Aleksandar and Rakonjac, Ljubinko and Milovanović, Jelena and Mladenović-Drinić, Snežana and Ristić, Danijela",
year = "2019",
abstract = "The process of plant breeding and conservation of gene pool among other things depends on the knowledge of the level of genetic differentiation. The aim of research in this paper was to determine the genetic differentiation of silver fir (Abies albaMill.) populations of regular  type  and  atypical  genotypes  with  pyramidal  crown  that  can  be  found  on  Javor mountain, at  the  site Ogorijevac. The  genetic  differentiation of silver fir  was determined using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave 29 alleles, while  the  average  number  of  alleles  was  3.2.  The  primer  NFH15  gave  the  smallest number of alleles (two), while the primer SF78 gave the greatest number of alleles (five). Dice  coefficient  of  the  genetic  similarity  was  used  to  obtain  a  dendrogram  by  UPMGA analysis  using  NTSYSpc  statistical  program.  The  genetic  similarity  recorded  among  the individuals P1 and P2 was the largest (0.89), while the populations VI and individual P2 showed the lowest similarity (0.61).Based on the cluster analysis it canbe concluded that the  studied populations and genotypes of silver  fir  with different types of crown are  clearly differentiated. The  basic insight  into  the  level  of  the  genetic  diversity  of  the  natural  populations  of  silver  fir  with the  various types of crown has been provided using selected SSR markers. The obtained results can be used for creating further strategy for the conservation of the available gene pool and the regeneration of silver fir forests in Serbia., Proces oplemenjivanja biljaka i konzervacije genofonda između ostalog, zavisi i od poznavanja stepena  genetičke  diferencijacije.  Cilj  istraživanja  u  ovom  radu  bio  je  da  se  utvrdi  genetička diferencijacija   populacija   jele   (Abies   alba Mill.)normalanog  tipa  i  atipičnih  genotipova piramidalanog tipa krošnje, koje se javljaju na planini Javor, na lokalitetu Ogorijevac. Genetička sličnost odnosno različitost jele određena je upotrebom SSR (Simple Sequence Repeat) markera. Devet SSR pari prajmera dalo je ukupno 29 alela, dok je prosečan broj alela bio 3,2. Prajmer NFH15 dao je najmanji broj alela (dva), dok je prajmer SF78 bio sa najvećim brojem alela (pet). Najviša vrednost koeficijenta genetičke sličnosti utvrđena je između genotipova P1 i P2 (0,89), dok je najniža vrednost koeficijenta genetičke sličnosti utvrđena između populacije VI i genotipa P2 (0,61).Koeficijenti  genetičke  sličnosti  po  Dice,  upotrebljeni  su  za  dobijanje  dendrograma  pomoću UPMGA  analize,  koristeći  NTSYSpc  statistički  program.  Na  osnovu  klaster  analize  može  se zaključiti  da  su  istraživane  populacije  i  genotipovi  jele  sa  različitim  tipom  krošnje  jasno izdiferencirani.  Upotrebom  izabranih  SSR  markera  dat  je  osnovni  uvid  u  nivo  genetičke raznovrsnosti prirodnih populacija jele različitog tipa krošnje. Dobijeni rezultati mogu poslužiti u budućoj strategiji na konzervaciji raspoloživog genofonda i obnavljanju jelovih šuma u Srbiji.",
publisher = "Beograd : Društvo genetičara Srbije, Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain, Procena genetičke diferencijacije jele (Abies alba Mill.) sa Javora pomoću SSR markera",
volume = "51",
number = "3",
pages = "1103-1112",
doi = "10.2298/GENSR1903103P"
}
Popović, V., Lučić, A., Rakonjac, L., Milovanović, J., Mladenović-Drinić, S.,& Ristić, D.. (2019). Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain. in Genetika
Beograd : Društvo genetičara Srbije., 51(3), 1103-1112.
https://doi.org/10.2298/GENSR1903103P
Popović V, Lučić A, Rakonjac L, Milovanović J, Mladenović-Drinić S, Ristić D. Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain. in Genetika. 2019;51(3):1103-1112.
doi:10.2298/GENSR1903103P .
Popović, Vladan, Lučić, Aleksandar, Rakonjac, Ljubinko, Milovanović, Jelena, Mladenović-Drinić, Snežana, Ristić, Danijela, "Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain" in Genetika, 51, no. 3 (2019):1103-1112,
https://doi.org/10.2298/GENSR1903103P . .
2
1

Application of different methods for measuring carotenoid status in maize grain

Kravic, Natalija; Mesarović, Jelena; Dragičević, Vesna; Ristić, Danijela; Anđelković, Violeta; Pećinar, Ilinka

(Belgrade : Serbian plant physiology society, 2018)

TY  - CONF
AU  - Kravic, Natalija
AU  - Mesarović, Jelena
AU  - Dragičević, Vesna
AU  - Ristić, Danijela
AU  - Anđelković, Violeta
AU  - Pećinar, Ilinka
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/987
AB  - Maize grain carotenoids are among the most abundant in cereals, exhibiting considerable diversity in the composition profiles. In this experiment, HPLC-DAD was used for lutein and zeaxanthin (predominant carotenoids) and β-carotene (proVA carotenoid) determination in flour of
whole maize kernel. In addition, Raman spectroscopy, as a non-destructive method for pretreatment-free and rapid in situ screening of carotenoids status in different kernel regions, was applied.
Six yellow maize inbred lines (L1-L6) were evaluated. After the spectral normalization, deconvolution was performed on three individual bands in three observed regions on kernel longitudinal
section (aleurone cell layer, floury and vitreous endosperm), for all genotypes. It was found that
the mean fitted area and intensity of three main carotenoid bands were the highest at 1520 cm-1,
medium at 1155 cm-1 and the lowest for the band at 1007 cm-1, as spectral features used to identify carotenoids and quantify relative carotenoid concentration. Both the highest values for mean
band fitted area and intensity observed in the floury endosperm, and the lowest in the aleurone
cell layer, were recorded in the same regions for all genotypes evaluated. L1 and L2 exhibited the
highest values, while L3 and L4 exhibited the lowest values for the average and total band area.
Significant and negative correlation between carotenoid content quantified by HPLC-DAD and
bands area fit for vitreous endosperm region obtained by Raman spectroscopy (-0.847; p≤0.05),
and between carotenoid content and total bands area (-0.898; p≤0.05) indicated the competition
for carbon supplies reflected through increased starch reposition over carotenoids accumulation.
PB  - Belgrade : Serbian plant physiology society
C3  - 3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts
T1  - Application of different methods for measuring carotenoid status in maize grain
ER  - 
@conference{
author = "Kravic, Natalija and Mesarović, Jelena and Dragičević, Vesna and Ristić, Danijela and Anđelković, Violeta and Pećinar, Ilinka",
year = "2018",
abstract = "Maize grain carotenoids are among the most abundant in cereals, exhibiting considerable diversity in the composition profiles. In this experiment, HPLC-DAD was used for lutein and zeaxanthin (predominant carotenoids) and β-carotene (proVA carotenoid) determination in flour of
whole maize kernel. In addition, Raman spectroscopy, as a non-destructive method for pretreatment-free and rapid in situ screening of carotenoids status in different kernel regions, was applied.
Six yellow maize inbred lines (L1-L6) were evaluated. After the spectral normalization, deconvolution was performed on three individual bands in three observed regions on kernel longitudinal
section (aleurone cell layer, floury and vitreous endosperm), for all genotypes. It was found that
the mean fitted area and intensity of three main carotenoid bands were the highest at 1520 cm-1,
medium at 1155 cm-1 and the lowest for the band at 1007 cm-1, as spectral features used to identify carotenoids and quantify relative carotenoid concentration. Both the highest values for mean
band fitted area and intensity observed in the floury endosperm, and the lowest in the aleurone
cell layer, were recorded in the same regions for all genotypes evaluated. L1 and L2 exhibited the
highest values, while L3 and L4 exhibited the lowest values for the average and total band area.
Significant and negative correlation between carotenoid content quantified by HPLC-DAD and
bands area fit for vitreous endosperm region obtained by Raman spectroscopy (-0.847; p≤0.05),
and between carotenoid content and total bands area (-0.898; p≤0.05) indicated the competition
for carbon supplies reflected through increased starch reposition over carotenoids accumulation.",
publisher = "Belgrade : Serbian plant physiology society",
journal = "3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts",
title = "Application of different methods for measuring carotenoid status in maize grain"
}
Kravic, N., Mesarović, J., Dragičević, V., Ristić, D., Anđelković, V.,& Pećinar, I.. (2018). Application of different methods for measuring carotenoid status in maize grain. in 3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts
Belgrade : Serbian plant physiology society..
Kravic N, Mesarović J, Dragičević V, Ristić D, Anđelković V, Pećinar I. Application of different methods for measuring carotenoid status in maize grain. in 3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts. 2018;..
Kravic, Natalija, Mesarović, Jelena, Dragičević, Vesna, Ristić, Danijela, Anđelković, Violeta, Pećinar, Ilinka, "Application of different methods for measuring carotenoid status in maize grain" in 3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts (2018).

Estimation of genetic diversity among maize inbred lines

Ristić, Danijela; Ignjatovic-Micic, Dragana; Mladenović Drinić, Snežana; Obradović, Ana; Kostadinović, Marija; Stevanović, Milan; Stanković, Goran

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2018)

TY  - CONF
AU  - Ristić, Danijela
AU  - Ignjatovic-Micic, Dragana
AU  - Mladenović Drinić, Snežana
AU  - Obradović, Ana
AU  - Kostadinović, Marija
AU  - Stevanović, Milan
AU  - Stanković, Goran
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/976
AB  - One of the most effective method in maize (Zea mays L) selection of adapted material is to
create synthetic populations from inbred lines of known origin and superior properties. The
methods used for inter- and intra-population synthetic improvement are some of the recurrent
selection techniques. Success of recurrent selection depends on the choice of parent
components and the method that will be used to obtain new inbred lines. The aim of this paper
was to apply molecular markers for estimation of genetic variability of inbred lines, as
potential parent components of synthetic populations. Molecular characterization of 26 inbred
lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total
number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036,
phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were
calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity
value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26)
was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three
groups mostly in accordance with their origin. The variability detected using SSR markers
could be useful in selecting best parental combinations in creating synthetic populations.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina
T1  - Estimation of genetic diversity among maize inbred lines
SP  - 280
EP  - 284
ER  - 
@conference{
author = "Ristić, Danijela and Ignjatovic-Micic, Dragana and Mladenović Drinić, Snežana and Obradović, Ana and Kostadinović, Marija and Stevanović, Milan and Stanković, Goran",
year = "2018",
abstract = "One of the most effective method in maize (Zea mays L) selection of adapted material is to
create synthetic populations from inbred lines of known origin and superior properties. The
methods used for inter- and intra-population synthetic improvement are some of the recurrent
selection techniques. Success of recurrent selection depends on the choice of parent
components and the method that will be used to obtain new inbred lines. The aim of this paper
was to apply molecular markers for estimation of genetic variability of inbred lines, as
potential parent components of synthetic populations. Molecular characterization of 26 inbred
lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total
number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036,
phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were
calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity
value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26)
was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three
groups mostly in accordance with their origin. The variability detected using SSR markers
could be useful in selecting best parental combinations in creating synthetic populations.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina",
title = "Estimation of genetic diversity among maize inbred lines",
pages = "280-284"
}
Ristić, D., Ignjatovic-Micic, D., Mladenović Drinić, S., Obradović, A., Kostadinović, M., Stevanović, M.,& Stanković, G.. (2018). Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 280-284.
Ristić D, Ignjatovic-Micic D, Mladenović Drinić S, Obradović A, Kostadinović M, Stevanović M, Stanković G. Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina. 2018;:280-284..
Ristić, Danijela, Ignjatovic-Micic, Dragana, Mladenović Drinić, Snežana, Obradović, Ana, Kostadinović, Marija, Stevanović, Milan, Stanković, Goran, "Estimation of genetic diversity among maize inbred lines" in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina (2018):280-284.

Parental polymorphism analysis in marker assisted selection for β-carotene rich maize

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Ristić, Danijela; Obradović, Ana; Stevanović, Milan; Mladenović-Drinić, Snežana

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2018)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Obradović, Ana
AU  - Stevanović, Milan
AU  - Mladenović-Drinić, Snežana
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/960
AB  - Marker assisted selection (MAS) is widely implemented into modern grain breeding programs. Molecular markers are used in foreground selection to control the target gene, as well as in background selection to accelerate the reconstruction of the recurrent parent genotype. The best results have been achieved with the qualitative traits, regulated by the action of a single or several genes and clearly phenotypically defined. One successful example of MAS is the improvement of β-carotene content using crtRB1 specific molecular marker. Maize Research Institute "Zemun Polje" has a breeding program aimed at conversion of standard maize to β-carotene rich genotypes adapted to temperate regions. The objectives of this study were to test the utility of gene-specific SSR marker in foreground selection and to identify polymorphic markers between parental lines to be used in background selection. Genetic variability between two standard and three high β-carotene parental inbred lines was analyzed with 40 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 77, average being 2.57. The genetic similarity values calculated on Dice coefficient ranged from 0.49 to 0.66. Parental polymorphism for crtRB1 showed a 543 bp fragment in donor lines, whereas a distinct 296 bp amplicon and a faint 1221 bp amplicon were generated in the recurrent parents. This marker will be used as foreground selection marker for the crtRB1 gene in the conversion of standard maize to β-carotene enriched lines for growing in temperate regions.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings
T1  - Parental polymorphism analysis in marker assisted selection for β-carotene rich maize
SP  - 333
EP  - 338
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Obradović, Ana and Stevanović, Milan and Mladenović-Drinić, Snežana",
year = "2018",
abstract = "Marker assisted selection (MAS) is widely implemented into modern grain breeding programs. Molecular markers are used in foreground selection to control the target gene, as well as in background selection to accelerate the reconstruction of the recurrent parent genotype. The best results have been achieved with the qualitative traits, regulated by the action of a single or several genes and clearly phenotypically defined. One successful example of MAS is the improvement of β-carotene content using crtRB1 specific molecular marker. Maize Research Institute "Zemun Polje" has a breeding program aimed at conversion of standard maize to β-carotene rich genotypes adapted to temperate regions. The objectives of this study were to test the utility of gene-specific SSR marker in foreground selection and to identify polymorphic markers between parental lines to be used in background selection. Genetic variability between two standard and three high β-carotene parental inbred lines was analyzed with 40 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 77, average being 2.57. The genetic similarity values calculated on Dice coefficient ranged from 0.49 to 0.66. Parental polymorphism for crtRB1 showed a 543 bp fragment in donor lines, whereas a distinct 296 bp amplicon and a faint 1221 bp amplicon were generated in the recurrent parents. This marker will be used as foreground selection marker for the crtRB1 gene in the conversion of standard maize to β-carotene enriched lines for growing in temperate regions.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings",
title = "Parental polymorphism analysis in marker assisted selection for β-carotene rich maize",
pages = "333-338"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D., Obradović, A., Stevanović, M.,& Mladenović-Drinić, S.. (2018). Parental polymorphism analysis in marker assisted selection for β-carotene rich maize. in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 333-338.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Obradović A, Stevanović M, Mladenović-Drinić S. Parental polymorphism analysis in marker assisted selection for β-carotene rich maize. in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings. 2018;:333-338..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Obradović, Ana, Stevanović, Milan, Mladenović-Drinić, Snežana, "Parental polymorphism analysis in marker assisted selection for β-carotene rich maize" in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings (2018):333-338.

Uticaj starosti semena na kvalitet različitih sorata lucerke

Štrbanović, Ratibor; Poštić, Dobrivoj; Ristić, Danijela; Marković, Jordan; Jovanović, Snežana; Tabaković, Marijenka; Stanisavljević, Rade

(Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi, 2018)

TY  - JOUR
AU  - Štrbanović, Ratibor
AU  - Poštić, Dobrivoj
AU  - Ristić, Danijela
AU  - Marković, Jordan
AU  - Jovanović, Snežana
AU  - Tabaković, Marijenka
AU  - Stanisavljević, Rade
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/946
AB  - Plava lucerka (Medicago sativa L.) je zbog svih svojih osobina najvažnija krmna biljka u 
našoj zemlji. Veliki značaj lucerke se ogleda u proizvodnji kvalitetne kabaste hrane, ali je i 
seme lucerke vredna i kurentna roba na domaćem i inostranom tržištu. Areal gajenja lucerke 
je na svim kontinentima u više od 80 zemalja, od umereno hladnog do tropskog pojasa. Široka 
geografska rasprostranjenost lucerke uslovljena je njenom velikom adaptabilnošću na različite 
klimatske i zemljišne uslove. Zasnivanje useva krmnih biljaka vrši se direktnom setvom
semena. Obzirom na te okolnosti, brza i uniformna pojava snaznih klijanaca lucerke je veoma 
značajna kako bi se osigurala visoka uniformnost klijanaca, što utiče na početnu fazu razvoja 
useva, a kao rezultat svega je visok i stabilan prinos. Sve ovo ukazuje na važnost izbora 
semena dobrog kvaliteta.U ovom radu je ispitivan uticaj starosti semena na kvalitet tri 
različite sorte lucerke (Kruševačka 28, NS-Banat i Čačanka-10). Seme ispitivanih sorata 
lucerke čuvano je pet godina u papirnim kesama u kontrolisanim uslovima temperature i 
relativne vlažnosti vazduha. Zatim je ispitivana klijavost semena različitih sorata lucerke 
starosti pet godina u klijalistu na temperaturi od 20o
C u mraku, deset dana (prema Pravilniku 
o ispitivanju kvaliteta semena poljoprivrednog bilja 47/87). Dobijeni rezultati ukazuju da je 
starost semena značajno uticala na ukupnu klijavost, nenormalne klijance, tvrdo i mrtvo seme. 
Najveća klijavost je ostvarena iz semena starog dve godine, pri tome uticaj sorte je takodje 
statistički značajno delovao na ukupnu klijavost, kao i interakcija starost semena x sorta 
(P≤0,05).
PB  - Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - 30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda
T1  - Uticaj starosti semena na kvalitet različitih sorata lucerke
SP  - 115
EP  - 115
ER  - 
@article{
author = "Štrbanović, Ratibor and Poštić, Dobrivoj and Ristić, Danijela and Marković, Jordan and Jovanović, Snežana and Tabaković, Marijenka and Stanisavljević, Rade",
year = "2018",
abstract = "Plava lucerka (Medicago sativa L.) je zbog svih svojih osobina najvažnija krmna biljka u 
našoj zemlji. Veliki značaj lucerke se ogleda u proizvodnji kvalitetne kabaste hrane, ali je i 
seme lucerke vredna i kurentna roba na domaćem i inostranom tržištu. Areal gajenja lucerke 
je na svim kontinentima u više od 80 zemalja, od umereno hladnog do tropskog pojasa. Široka 
geografska rasprostranjenost lucerke uslovljena je njenom velikom adaptabilnošću na različite 
klimatske i zemljišne uslove. Zasnivanje useva krmnih biljaka vrši se direktnom setvom
semena. Obzirom na te okolnosti, brza i uniformna pojava snaznih klijanaca lucerke je veoma 
značajna kako bi se osigurala visoka uniformnost klijanaca, što utiče na početnu fazu razvoja 
useva, a kao rezultat svega je visok i stabilan prinos. Sve ovo ukazuje na važnost izbora 
semena dobrog kvaliteta.U ovom radu je ispitivan uticaj starosti semena na kvalitet tri 
različite sorte lucerke (Kruševačka 28, NS-Banat i Čačanka-10). Seme ispitivanih sorata 
lucerke čuvano je pet godina u papirnim kesama u kontrolisanim uslovima temperature i 
relativne vlažnosti vazduha. Zatim je ispitivana klijavost semena različitih sorata lucerke 
starosti pet godina u klijalistu na temperaturi od 20o
C u mraku, deset dana (prema Pravilniku 
o ispitivanju kvaliteta semena poljoprivrednog bilja 47/87). Dobijeni rezultati ukazuju da je 
starost semena značajno uticala na ukupnu klijavost, nenormalne klijance, tvrdo i mrtvo seme. 
Najveća klijavost je ostvarena iz semena starog dve godine, pri tome uticaj sorte je takodje 
statistički značajno delovao na ukupnu klijavost, kao i interakcija starost semena x sorta 
(P≤0,05).",
publisher = "Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda",
title = "Uticaj starosti semena na kvalitet različitih sorata lucerke",
pages = "115-115"
}
Štrbanović, R., Poštić, D., Ristić, D., Marković, J., Jovanović, S., Tabaković, M.,& Stanisavljević, R.. (2018). Uticaj starosti semena na kvalitet različitih sorata lucerke. in 30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda
Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi., 115-115.
Štrbanović R, Poštić D, Ristić D, Marković J, Jovanović S, Tabaković M, Stanisavljević R. Uticaj starosti semena na kvalitet različitih sorata lucerke. in 30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda. 2018;:115-115..
Štrbanović, Ratibor, Poštić, Dobrivoj, Ristić, Danijela, Marković, Jordan, Jovanović, Snežana, Tabaković, Marijenka, Stanisavljević, Rade, "Uticaj starosti semena na kvalitet različitih sorata lucerke" in 30. Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda (2018):115-115.

Determination of free phenolic acids from leaves within different colored maize

Mesarović, Jelena; Dragičević, Vesna; Mladenović-Drinić, Snežana; Ristić, Danijela; Kravić, Natalija

(Srpsko hemijsko društvo, Beograd, 2017)

TY  - JOUR
AU  - Mesarović, Jelena
AU  - Dragičević, Vesna
AU  - Mladenović-Drinić, Snežana
AU  - Ristić, Danijela
AU  - Kravić, Natalija
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/678
AB  - Along with other plant parts, maize leaves are widely used for making fermented food for cattle, known as silage. Since there have only been a few reports on studies concerning the extraction and determination of phenolic acids from maize leaves, the main goal of this investigation was to evaluate the content of free phenolic acids in the leaves of fifteen different maize inbred lines. Reverse-phase, high performance liquid chromatography (RP-HPLC), with a photodiode array detector (DAD), was performed. Under the optimized chromatographic conditions, referring to short time of sample preparation, small quantities of solvent and direct injection of the extract into HPLC, phenolic acids (i.e., gallic, protocatechuic, caffeic, p-coumaric and ferulic acid) were successfully separated in less than 25 min, indicating that the method could be applied for routine analysis. The efficiency and validation of the method was evaluated by measuring the rate parameters: linearity, limit of detection and quantification, accuracy and precision. The obtained results showed that the most abundant free phenolic acid was p-coumaric acid (23.57 mu g g(-1) dry weight), followed by ferulic and caffeic acids (21.27 and 20.78 mu g g(-1) dry weight, respectively). Principal Component Analysis (PCA) revealed the existence of a link.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Determination of free phenolic acids from leaves within different colored maize
VL  - 82
IS  - 1
SP  - 63
EP  - 72
DO  - 10.2298/JSC160512104M
UR  - conv_949
ER  - 
@article{
author = "Mesarović, Jelena and Dragičević, Vesna and Mladenović-Drinić, Snežana and Ristić, Danijela and Kravić, Natalija",
year = "2017",
abstract = "Along with other plant parts, maize leaves are widely used for making fermented food for cattle, known as silage. Since there have only been a few reports on studies concerning the extraction and determination of phenolic acids from maize leaves, the main goal of this investigation was to evaluate the content of free phenolic acids in the leaves of fifteen different maize inbred lines. Reverse-phase, high performance liquid chromatography (RP-HPLC), with a photodiode array detector (DAD), was performed. Under the optimized chromatographic conditions, referring to short time of sample preparation, small quantities of solvent and direct injection of the extract into HPLC, phenolic acids (i.e., gallic, protocatechuic, caffeic, p-coumaric and ferulic acid) were successfully separated in less than 25 min, indicating that the method could be applied for routine analysis. The efficiency and validation of the method was evaluated by measuring the rate parameters: linearity, limit of detection and quantification, accuracy and precision. The obtained results showed that the most abundant free phenolic acid was p-coumaric acid (23.57 mu g g(-1) dry weight), followed by ferulic and caffeic acids (21.27 and 20.78 mu g g(-1) dry weight, respectively). Principal Component Analysis (PCA) revealed the existence of a link.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Determination of free phenolic acids from leaves within different colored maize",
volume = "82",
number = "1",
pages = "63-72",
doi = "10.2298/JSC160512104M",
url = "conv_949"
}
Mesarović, J., Dragičević, V., Mladenović-Drinić, S., Ristić, D.,& Kravić, N.. (2017). Determination of free phenolic acids from leaves within different colored maize. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 82(1), 63-72.
https://doi.org/10.2298/JSC160512104M
conv_949
Mesarović J, Dragičević V, Mladenović-Drinić S, Ristić D, Kravić N. Determination of free phenolic acids from leaves within different colored maize. in Journal of the Serbian Chemical Society. 2017;82(1):63-72.
doi:10.2298/JSC160512104M
conv_949 .
Mesarović, Jelena, Dragičević, Vesna, Mladenović-Drinić, Snežana, Ristić, Danijela, Kravić, Natalija, "Determination of free phenolic acids from leaves within different colored maize" in Journal of the Serbian Chemical Society, 82, no. 1 (2017):63-72,
https://doi.org/10.2298/JSC160512104M .,
conv_949 .
2
1
2

Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers

Popović, Vladan; Lučić, Aleksandar; Rakonjac, Ljubinko; Mladenović-Drinić, Snežana; Ristić, Danijela; Cvjetković, Branislav

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Popović, Vladan
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Mladenović-Drinić, Snežana
AU  - Ristić, Danijela
AU  - Cvjetković, Branislav
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/659
AB  - The paper presents the results of the analysis of genetic variability of eight populations of silver fir (Abies alba Mill.) in Serbia obtained using SSR markers. The genomic DNA was isolated from tissue of needles of all eight populations. Due to the costly and lengthy process a small number of the SSR markers for Abies alba have been developed, so in this study were used the microsatellite markers of related species. The obtained results indicate a low level of the genetic variability between natural populations of silver fir. The total number of alleles detected with nine SSR markers in eight studied populations of silver fir is 28. The range of alleles varies from two for NFF15 to six for SF78 with an average of 3.1 alleles per locus. The mean value of genetic similarity between populations is 0.92. The smallest genetic similarity between pairs of populations is 0.82 (Dubocica Bare and Stara Planina; Dubocica Bare and Tara) and the greatest genetic similarity is 1 (Zlatar and Stara Planina, Zlatar and Tara, Stara Planina and Tara). A basic insight into the level of genetic diversity of natural populations of silver fir in Serbia, which are located in a relatively small area, has been given using a set of SSR markers. The obtained results can be used in the future strategy for the management and regeneration of silver fir forests.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers
VL  - 49
IS  - 3
SP  - 979
EP  - 988
DO  - 10.2298/GENSR1703979P
UR  - conv_970
ER  - 
@article{
author = "Popović, Vladan and Lučić, Aleksandar and Rakonjac, Ljubinko and Mladenović-Drinić, Snežana and Ristić, Danijela and Cvjetković, Branislav",
year = "2017",
abstract = "The paper presents the results of the analysis of genetic variability of eight populations of silver fir (Abies alba Mill.) in Serbia obtained using SSR markers. The genomic DNA was isolated from tissue of needles of all eight populations. Due to the costly and lengthy process a small number of the SSR markers for Abies alba have been developed, so in this study were used the microsatellite markers of related species. The obtained results indicate a low level of the genetic variability between natural populations of silver fir. The total number of alleles detected with nine SSR markers in eight studied populations of silver fir is 28. The range of alleles varies from two for NFF15 to six for SF78 with an average of 3.1 alleles per locus. The mean value of genetic similarity between populations is 0.92. The smallest genetic similarity between pairs of populations is 0.82 (Dubocica Bare and Stara Planina; Dubocica Bare and Tara) and the greatest genetic similarity is 1 (Zlatar and Stara Planina, Zlatar and Tara, Stara Planina and Tara). A basic insight into the level of genetic diversity of natural populations of silver fir in Serbia, which are located in a relatively small area, has been given using a set of SSR markers. The obtained results can be used in the future strategy for the management and regeneration of silver fir forests.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers",
volume = "49",
number = "3",
pages = "979-988",
doi = "10.2298/GENSR1703979P",
url = "conv_970"
}
Popović, V., Lučić, A., Rakonjac, L., Mladenović-Drinić, S., Ristić, D.,& Cvjetković, B.. (2017). Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers. in Genetika
Društvo genetičara Srbije, Beograd., 49(3), 979-988.
https://doi.org/10.2298/GENSR1703979P
conv_970
Popović V, Lučić A, Rakonjac L, Mladenović-Drinić S, Ristić D, Cvjetković B. Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers. in Genetika. 2017;49(3):979-988.
doi:10.2298/GENSR1703979P
conv_970 .
Popović, Vladan, Lučić, Aleksandar, Rakonjac, Ljubinko, Mladenović-Drinić, Snežana, Ristić, Danijela, Cvjetković, Branislav, "Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers" in Genetika, 49, no. 3 (2017):979-988,
https://doi.org/10.2298/GENSR1703979P .,
conv_970 .
2
4
4

Maize landraces as a source of adaptation to climatic change

Anđelković, Violeta; Ristić, Danijela; Babić, Vojka; Dumanović, Zoran; Kravić, Natalija

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2016)

TY  - JOUR
AU  - Anđelković, Violeta
AU  - Ristić, Danijela
AU  - Babić, Vojka
AU  - Dumanović, Zoran
AU  - Kravić, Natalija
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/657
AB  - Predictions of global warming point out that frequency and severity of temperature and rainfall extremes are expected to increase in the following decades. Yield stability could be achieved by improvement in future breeding based on information of cereal reproduction under changed environmental conditions. Landraces are valuable sources of beneficial alleles, e.g. tolerance to drought and improved grain yield, for introgression into adapted germplasm. Twenty-six drought tolerant maize landraces, selected among gene bank accessions and previously tested under controlled drought conditions and in temperate climate, were evaluated, at two sites and two plant densities in Zemun Polje. Since 2014 was with enormous rainfalls and flooding, morphological and agronomical traits were measured. Obtained data, together with the results from dry 2012, were plotted for principal component analysis. One local landrace and two introduced landraces, medium in maturity, were selected according to PC analysis and achieved grain yield that was stable across different climatic conditions.
AB  - Procenjuje se da će u narednim decenijama, usled globalnog zagrevanja, doći do učestalije pojave ekstremnih temperatura i padavina. Ostvarivanje stabilnih prinosa u budućim programima oplemenjivanja zavisiće od informacija o mogućnostima reprodukcije useva u promenjenim uslovima spoljašnje sredine. Populacije predstavljaju dragocen izvor poželjnih alela (npr. za tolerantnost prema suši i poboljšanje prinosa) za introgresiju u adaptiranu germplazmu. Dvadeset šest populacija kukuruza, tolerantnih na sušu, odabranih iz banke gena i prethodno testiranih u uslovima kontrolisanog stresa suše, kao i u umerenom klimatu, testirane su na dva lokaliteta i u dve različite gustine u Zemun Polju. U 2014. godini koju su obeležile velike količine padavina i poplave, merena su morfološka i agronomska svojstva. Dobijeni podaci, zajedno sa podacima iz sušne 2012. godine obrađeni su PCA metodom (analiza glavnih komponenti). Jedna lokalna i dve introdukované, srednjestasne populacije, odabrane su na osnovu PC analize i ostvarenog prinosa zrna, koji je bio stabilan bez većih variranja, u različitim uslovima spoljašnje sredine.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Maize landraces as a source of adaptation to climatic change
T1  - Populacije kukuruza kao izvor adaptabilnosti na klimatske promene
VL  - 53
IS  - 1
SP  - 24
EP  - 29
DO  - 10.5937/ratpov53-9138
UR  - conv_621
ER  - 
@article{
author = "Anđelković, Violeta and Ristić, Danijela and Babić, Vojka and Dumanović, Zoran and Kravić, Natalija",
year = "2016",
abstract = "Predictions of global warming point out that frequency and severity of temperature and rainfall extremes are expected to increase in the following decades. Yield stability could be achieved by improvement in future breeding based on information of cereal reproduction under changed environmental conditions. Landraces are valuable sources of beneficial alleles, e.g. tolerance to drought and improved grain yield, for introgression into adapted germplasm. Twenty-six drought tolerant maize landraces, selected among gene bank accessions and previously tested under controlled drought conditions and in temperate climate, were evaluated, at two sites and two plant densities in Zemun Polje. Since 2014 was with enormous rainfalls and flooding, morphological and agronomical traits were measured. Obtained data, together with the results from dry 2012, were plotted for principal component analysis. One local landrace and two introduced landraces, medium in maturity, were selected according to PC analysis and achieved grain yield that was stable across different climatic conditions., Procenjuje se da će u narednim decenijama, usled globalnog zagrevanja, doći do učestalije pojave ekstremnih temperatura i padavina. Ostvarivanje stabilnih prinosa u budućim programima oplemenjivanja zavisiće od informacija o mogućnostima reprodukcije useva u promenjenim uslovima spoljašnje sredine. Populacije predstavljaju dragocen izvor poželjnih alela (npr. za tolerantnost prema suši i poboljšanje prinosa) za introgresiju u adaptiranu germplazmu. Dvadeset šest populacija kukuruza, tolerantnih na sušu, odabranih iz banke gena i prethodno testiranih u uslovima kontrolisanog stresa suše, kao i u umerenom klimatu, testirane su na dva lokaliteta i u dve različite gustine u Zemun Polju. U 2014. godini koju su obeležile velike količine padavina i poplave, merena su morfološka i agronomska svojstva. Dobijeni podaci, zajedno sa podacima iz sušne 2012. godine obrađeni su PCA metodom (analiza glavnih komponenti). Jedna lokalna i dve introdukované, srednjestasne populacije, odabrane su na osnovu PC analize i ostvarenog prinosa zrna, koji je bio stabilan bez većih variranja, u različitim uslovima spoljašnje sredine.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Maize landraces as a source of adaptation to climatic change, Populacije kukuruza kao izvor adaptabilnosti na klimatske promene",
volume = "53",
number = "1",
pages = "24-29",
doi = "10.5937/ratpov53-9138",
url = "conv_621"
}
Anđelković, V., Ristić, D., Babić, V., Dumanović, Z.,& Kravić, N.. (2016). Maize landraces as a source of adaptation to climatic change. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 53(1), 24-29.
https://doi.org/10.5937/ratpov53-9138
conv_621
Anđelković V, Ristić D, Babić V, Dumanović Z, Kravić N. Maize landraces as a source of adaptation to climatic change. in Ratarstvo i povrtarstvo. 2016;53(1):24-29.
doi:10.5937/ratpov53-9138
conv_621 .
Anđelković, Violeta, Ristić, Danijela, Babić, Vojka, Dumanović, Zoran, Kravić, Natalija, "Maize landraces as a source of adaptation to climatic change" in Ratarstvo i povrtarstvo, 53, no. 1 (2016):24-29,
https://doi.org/10.5937/ratpov53-9138 .,
conv_621 .
2

Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.)

Pavlov, Jovan; Delić, Nenad; Ristić, Danijela; Čamdžija, Zoran; Stevanović, Milan; Tolimir, Miodrag; Zivanović, Tomislav

(Društvo genetičara Srbije, Beograd, 2016)

TY  - JOUR
AU  - Pavlov, Jovan
AU  - Delić, Nenad
AU  - Ristić, Danijela
AU  - Čamdžija, Zoran
AU  - Stevanović, Milan
AU  - Tolimir, Miodrag
AU  - Zivanović, Tomislav
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/647
AB  - Six maize inbred lines for which we assumed that have a similar genetic background were selected for the study. Inbred lines were crossed according to incomplete diallel design and fifteen hybrid combinations were obtained. Hybrid combinations and their parental lines were used in our study. The objective of the study was to examine genetic polymorphism of parental lines, as well as to determine relationships between SSR genetic distance and values of high parent heterosis and specific combining abilities for grain yield. By using 19 SSR primers we classified inbred lines in two groups. First cluster consists of inbred lines L1, L2, L3, L4 and L5, while inbred line L6 formed second cluster, indicating different heterotic background in comparison to the other five inbred lines. The Spearman's correlation coefficients between SSR based genetic distance and specific combining abilities for grain yield had a value of (r=0,53*), while positive correlation were also found between genetic distance and high parent heterosis (r=0,57*).
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.)
VL  - 48
IS  - 1
SP  - 165
EP  - 172
DO  - 10.2298/GENSR1601165P
UR  - conv_929
ER  - 
@article{
author = "Pavlov, Jovan and Delić, Nenad and Ristić, Danijela and Čamdžija, Zoran and Stevanović, Milan and Tolimir, Miodrag and Zivanović, Tomislav",
year = "2016",
abstract = "Six maize inbred lines for which we assumed that have a similar genetic background were selected for the study. Inbred lines were crossed according to incomplete diallel design and fifteen hybrid combinations were obtained. Hybrid combinations and their parental lines were used in our study. The objective of the study was to examine genetic polymorphism of parental lines, as well as to determine relationships between SSR genetic distance and values of high parent heterosis and specific combining abilities for grain yield. By using 19 SSR primers we classified inbred lines in two groups. First cluster consists of inbred lines L1, L2, L3, L4 and L5, while inbred line L6 formed second cluster, indicating different heterotic background in comparison to the other five inbred lines. The Spearman's correlation coefficients between SSR based genetic distance and specific combining abilities for grain yield had a value of (r=0,53*), while positive correlation were also found between genetic distance and high parent heterosis (r=0,57*).",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.)",
volume = "48",
number = "1",
pages = "165-172",
doi = "10.2298/GENSR1601165P",
url = "conv_929"
}
Pavlov, J., Delić, N., Ristić, D., Čamdžija, Z., Stevanović, M., Tolimir, M.,& Zivanović, T.. (2016). Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.). in Genetika
Društvo genetičara Srbije, Beograd., 48(1), 165-172.
https://doi.org/10.2298/GENSR1601165P
conv_929
Pavlov J, Delić N, Ristić D, Čamdžija Z, Stevanović M, Tolimir M, Zivanović T. Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.). in Genetika. 2016;48(1):165-172.
doi:10.2298/GENSR1601165P
conv_929 .
Pavlov, Jovan, Delić, Nenad, Ristić, Danijela, Čamdžija, Zoran, Stevanović, Milan, Tolimir, Miodrag, Zivanović, Tomislav, "Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays l.)" in Genetika, 48, no. 1 (2016):165-172,
https://doi.org/10.2298/GENSR1601165P .,
conv_929 .
2
4
5

Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits

Kostadinović, Marija; Ignjatović-Micić, Dragana; Ristić, Danijela; Mladenović-Drinić, Snežana; Vančetović, Jelena; Božinović, Sofija; Stanković, Goran

(Public Library Science, San Francisco, 2016)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Stanković, Goran
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/621
AB  - Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.
PB  - Public Library Science, San Francisco
T2  - PLOS ONE
T1  - Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167635
UR  - conv_941
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Ristić, Danijela and Mladenović-Drinić, Snežana and Vančetović, Jelena and Božinović, Sofija and Stanković, Goran",
year = "2016",
abstract = "Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.",
publisher = "Public Library Science, San Francisco",
journal = "PLOS ONE",
title = "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167635",
url = "conv_941"
}
Kostadinović, M., Ignjatović-Micić, D., Ristić, D., Mladenović-Drinić, S., Vančetović, J., Božinović, S.,& Stanković, G.. (2016). Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167635
conv_941
Kostadinović M, Ignjatović-Micić D, Ristić D, Mladenović-Drinić S, Vančetović J, Božinović S, Stanković G. Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE. 2016;11(12).
doi:10.1371/journal.pone.0167635
conv_941 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Ristić, Danijela, Mladenović-Drinić, Snežana, Vančetović, Jelena, Božinović, Sofija, Stanković, Goran, "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits" in PLOS ONE, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167635 .,
conv_941 .
1
7
7
8

Grain yield and phenotypic performances of maize inbreds under variable environmental conditions

Kravić, Natalija; Babić, Vojka; Ristić, Danijela; Srdić, Jelena; Anđelković, Violeta

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2015)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Ristić, Danijela
AU  - Srdić, Jelena
AU  - Anđelković, Violeta
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/596
AB  - According to regional reports on climate, more frequent and severe droughts and floods are already apparent. Modern maize breeding for different purposes (e.g. drought tolerance) emphasizes the importance for improved maize ability to withstand adverse environmental conditions. Genotypes with drought tolerance obtain high grain yield under optimal conditions and lower, but stable yield under dry environmental conditions. Field testing was conducted in 2014, on twenty-three maize inbred lines differing in drought tolerance. The inbreds were evaluated in respect to morphological traits (plant and ear height, total number of leaves, number of leaves above uppermost ear, ear leaf length and width), yield components and grain yield per plant. The experiment was set up according to RCB design with two replications. To simulate water stress, two plant densities were applied. According to Principal Component Analysis and cluster analysis, five inbred lines (T1, T2, T4, T8 i T15) performed the highest stability in both experimental conditions. Those inbreds could be used as a source for drought tolerance in breeding programs.
AB  - Regionalna istraživanja klimatskih promena ukazuju na sve učestaliju pojavu promena u režimu padavina i temperatura. Za potrebe savremene selekcije za različite osobine (npr. tolerantnost prema suši), poboljšana sposobnost kukuruza da izdrži nepovoljne uslove spoljašnje sredine je esencijalna. U optimalnim uslovima, genotipovi tolerantni na sušu ostvaruju visok prinos, dok u uslovima vodnog deficita u kombinaciji sa visokim temperaturama vazduha ostvaruju niži, ali stabilan prinos. U poljskim ogledima 2014, izvršena je evaluacija dvadeset tri inbred linije kukuruza različite tolerantnosti na stres suše, praćenjem morfoloških osobina (visine biljke i gornjeg klipa, ukupnog broja listova i broja listova iznad gornjeg klipa, dižine i širine klipnog lista), komponenata prinosa i prinosa zrna po biljci. Ogled je sejan po kompletno randomiziranom blok dizajnu, u dva ponavljanja. Za simulaciju vodnog deficita, ogled je sejan u dve gustine. Poređenjem rezultata iz obe eksperimentalne gustine, dobijenih analizom glavnih komponenata i klaster analizom, pet samooplodnih linija kukuruza (T1, T2, T4, T8 i T15) su pokazale najveću stabilnost i mogu se smatrati poželjnim izvorima tolerantnosti prema suši za potrebe oplemenjivanja na ovo svojstvo.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Grain yield and phenotypic performances of maize inbreds under variable environmental conditions
T1  - Prinos i fenotipske karakteristike inbred linija kukuruza u promenljivim uslovima spoljašnje sredine
VL  - 21
IS  - 2
SP  - 1
EP  - 10
DO  - 10.5937/SelSem1502001K
UR  - conv_300
ER  - 
@article{
author = "Kravić, Natalija and Babić, Vojka and Ristić, Danijela and Srdić, Jelena and Anđelković, Violeta",
year = "2015",
abstract = "According to regional reports on climate, more frequent and severe droughts and floods are already apparent. Modern maize breeding for different purposes (e.g. drought tolerance) emphasizes the importance for improved maize ability to withstand adverse environmental conditions. Genotypes with drought tolerance obtain high grain yield under optimal conditions and lower, but stable yield under dry environmental conditions. Field testing was conducted in 2014, on twenty-three maize inbred lines differing in drought tolerance. The inbreds were evaluated in respect to morphological traits (plant and ear height, total number of leaves, number of leaves above uppermost ear, ear leaf length and width), yield components and grain yield per plant. The experiment was set up according to RCB design with two replications. To simulate water stress, two plant densities were applied. According to Principal Component Analysis and cluster analysis, five inbred lines (T1, T2, T4, T8 i T15) performed the highest stability in both experimental conditions. Those inbreds could be used as a source for drought tolerance in breeding programs., Regionalna istraživanja klimatskih promena ukazuju na sve učestaliju pojavu promena u režimu padavina i temperatura. Za potrebe savremene selekcije za različite osobine (npr. tolerantnost prema suši), poboljšana sposobnost kukuruza da izdrži nepovoljne uslove spoljašnje sredine je esencijalna. U optimalnim uslovima, genotipovi tolerantni na sušu ostvaruju visok prinos, dok u uslovima vodnog deficita u kombinaciji sa visokim temperaturama vazduha ostvaruju niži, ali stabilan prinos. U poljskim ogledima 2014, izvršena je evaluacija dvadeset tri inbred linije kukuruza različite tolerantnosti na stres suše, praćenjem morfoloških osobina (visine biljke i gornjeg klipa, ukupnog broja listova i broja listova iznad gornjeg klipa, dižine i širine klipnog lista), komponenata prinosa i prinosa zrna po biljci. Ogled je sejan po kompletno randomiziranom blok dizajnu, u dva ponavljanja. Za simulaciju vodnog deficita, ogled je sejan u dve gustine. Poređenjem rezultata iz obe eksperimentalne gustine, dobijenih analizom glavnih komponenata i klaster analizom, pet samooplodnih linija kukuruza (T1, T2, T4, T8 i T15) su pokazale najveću stabilnost i mogu se smatrati poželjnim izvorima tolerantnosti prema suši za potrebe oplemenjivanja na ovo svojstvo.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Grain yield and phenotypic performances of maize inbreds under variable environmental conditions, Prinos i fenotipske karakteristike inbred linija kukuruza u promenljivim uslovima spoljašnje sredine",
volume = "21",
number = "2",
pages = "1-10",
doi = "10.5937/SelSem1502001K",
url = "conv_300"
}
Kravić, N., Babić, V., Ristić, D., Srdić, J.,& Anđelković, V.. (2015). Grain yield and phenotypic performances of maize inbreds under variable environmental conditions. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 21(2), 1-10.
https://doi.org/10.5937/SelSem1502001K
conv_300
Kravić N, Babić V, Ristić D, Srdić J, Anđelković V. Grain yield and phenotypic performances of maize inbreds under variable environmental conditions. in Selekcija i semenarstvo. 2015;21(2):1-10.
doi:10.5937/SelSem1502001K
conv_300 .
Kravić, Natalija, Babić, Vojka, Ristić, Danijela, Srdić, Jelena, Anđelković, Violeta, "Grain yield and phenotypic performances of maize inbreds under variable environmental conditions" in Selekcija i semenarstvo, 21, no. 2 (2015):1-10,
https://doi.org/10.5937/SelSem1502001K .,
conv_300 .