Estimation of genetic diversity among maize inbred lines
Authors
Ristić, DanijelaIgnjatovic-Micic, Dragana
Mladenović Drinić, Snežana
Obradović, Ana
Kostadinović, Marija
Stevanović, Milan
Stanković, Goran
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One of the most effective method in maize (Zea mays L) selection of adapted material is to
create synthetic populations from inbred lines of known origin and superior properties. The
methods used for inter- and intra-population synthetic improvement are some of the recurrent
selection techniques. Success of recurrent selection depends on the choice of parent
components and the method that will be used to obtain new inbred lines. The aim of this paper
was to apply molecular markers for estimation of genetic variability of inbred lines, as
potential parent components of synthetic populations. Molecular characterization of 26 inbred
lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total
number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036,
phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were
calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity
...value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26)
was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three
groups mostly in accordance with their origin. The variability detected using SSR markers
could be useful in selecting best parental combinations in creating synthetic populations.
Keywords:
inbred lines / synthetic population / SSR markersSource:
9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina, 2018, 280-284Publisher:
- Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
Funding / projects:
- Exploitation of maize diversity to improve grain quality and drought tolerance (RS-MESTD-Technological Development (TD or TR)-31028)
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MRIZPTY - CONF AU - Ristić, Danijela AU - Ignjatovic-Micic, Dragana AU - Mladenović Drinić, Snežana AU - Obradović, Ana AU - Kostadinović, Marija AU - Stevanović, Milan AU - Stanković, Goran PY - 2018 UR - http://rik.mrizp.rs/handle/123456789/976 AB - One of the most effective method in maize (Zea mays L) selection of adapted material is to create synthetic populations from inbred lines of known origin and superior properties. The methods used for inter- and intra-population synthetic improvement are some of the recurrent selection techniques. Success of recurrent selection depends on the choice of parent components and the method that will be used to obtain new inbred lines. The aim of this paper was to apply molecular markers for estimation of genetic variability of inbred lines, as potential parent components of synthetic populations. Molecular characterization of 26 inbred lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036, phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26) was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three groups mostly in accordance with their origin. The variability detected using SSR markers could be useful in selecting best parental combinations in creating synthetic populations. PB - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia C3 - 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina T1 - Estimation of genetic diversity among maize inbred lines SP - 280 EP - 284 UR - https://hdl.handle.net/21.15107/rcub_rik_976 ER -
@conference{ author = "Ristić, Danijela and Ignjatovic-Micic, Dragana and Mladenović Drinić, Snežana and Obradović, Ana and Kostadinović, Marija and Stevanović, Milan and Stanković, Goran", year = "2018", abstract = "One of the most effective method in maize (Zea mays L) selection of adapted material is to create synthetic populations from inbred lines of known origin and superior properties. The methods used for inter- and intra-population synthetic improvement are some of the recurrent selection techniques. Success of recurrent selection depends on the choice of parent components and the method that will be used to obtain new inbred lines. The aim of this paper was to apply molecular markers for estimation of genetic variability of inbred lines, as potential parent components of synthetic populations. Molecular characterization of 26 inbred lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036, phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26) was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three groups mostly in accordance with their origin. The variability detected using SSR markers could be useful in selecting best parental combinations in creating synthetic populations.", publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia", journal = "9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina", title = "Estimation of genetic diversity among maize inbred lines", pages = "280-284", url = "https://hdl.handle.net/21.15107/rcub_rik_976" }
Ristić, D., Ignjatovic-Micic, D., Mladenović Drinić, S., Obradović, A., Kostadinović, M., Stevanović, M.,& Stanković, G.. (2018). Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 280-284. https://hdl.handle.net/21.15107/rcub_rik_976
Ristić D, Ignjatovic-Micic D, Mladenović Drinić S, Obradović A, Kostadinović M, Stevanović M, Stanković G. Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina. 2018;:280-284. https://hdl.handle.net/21.15107/rcub_rik_976 .
Ristić, Danijela, Ignjatovic-Micic, Dragana, Mladenović Drinić, Snežana, Obradović, Ana, Kostadinović, Marija, Stevanović, Milan, Stanković, Goran, "Estimation of genetic diversity among maize inbred lines" in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina (2018):280-284, https://hdl.handle.net/21.15107/rcub_rik_976 .