Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant
2010
Authors
Dodig, DejanZorić, Miroslav
Lalević, Blazo
Šurlan-Momirović, Gordana
Mitić, Nevena
Nikolić, Radomirka
King, Stephen R.
Article (Published version)
Metadata
Show full item recordAbstract
Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR traits and to determine the period of wheat growth during which these factors were the most effective. The genotypes were grown in an experimental field during three seasons differing in meteorological conditions. The relationships between TCR traits and climatic factors within three time periods of wheat growth: 2, 6 and 10 weeks prior to embryo sampling were analysed by biplot analysis. The tissu...e culture traits were influenced at very different degrees by climatic factors: from 16.8% (RC) to 69.8% (CF). Donor plant environment with high temperatures and low rainfalls reduced (p lt 0.05) the tissue culture performance of wheat genotypes. Callus formation was most sensitive to the temperature based factors. The environmental conditions between flowering and the medium milk stage were the most important for CF, while RC and PPE were not particularly related to any period.
Keywords:
Triticum aestivum L. / biplot / correlation analysis / environment variable / growth periodSource:
Scientia Agricola, 2010, 67, 3, 295-300Publisher:
- Univ Sao Paolo, Cerquera Cesar
Funding / projects:
- Regulacija morfogenetskih procesa i sekundarnog metabolizma i genetičke transformacije biljaka u kulturi in vitro (RS-MESTD-MPN2006-2010-143026)
DOI: 10.1590/S0103-90162010000300007
ISSN: 0103-9016
WoS: 000278958200007
Scopus: 2-s2.0-77954184090
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Institution/Community
MRIZPTY - JOUR AU - Dodig, Dejan AU - Zorić, Miroslav AU - Lalević, Blazo AU - Šurlan-Momirović, Gordana AU - Mitić, Nevena AU - Nikolić, Radomirka AU - King, Stephen R. PY - 2010 UR - http://rik.mrizp.rs/handle/123456789/339 AB - Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR traits and to determine the period of wheat growth during which these factors were the most effective. The genotypes were grown in an experimental field during three seasons differing in meteorological conditions. The relationships between TCR traits and climatic factors within three time periods of wheat growth: 2, 6 and 10 weeks prior to embryo sampling were analysed by biplot analysis. The tissue culture traits were influenced at very different degrees by climatic factors: from 16.8% (RC) to 69.8% (CF). Donor plant environment with high temperatures and low rainfalls reduced (p lt 0.05) the tissue culture performance of wheat genotypes. Callus formation was most sensitive to the temperature based factors. The environmental conditions between flowering and the medium milk stage were the most important for CF, while RC and PPE were not particularly related to any period. PB - Univ Sao Paolo, Cerquera Cesar T2 - Scientia Agricola T1 - Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant VL - 67 IS - 3 SP - 295 EP - 300 DO - 10.1590/S0103-90162010000300007 ER -
@article{ author = "Dodig, Dejan and Zorić, Miroslav and Lalević, Blazo and Šurlan-Momirović, Gordana and Mitić, Nevena and Nikolić, Radomirka and King, Stephen R.", year = "2010", abstract = "Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR traits and to determine the period of wheat growth during which these factors were the most effective. The genotypes were grown in an experimental field during three seasons differing in meteorological conditions. The relationships between TCR traits and climatic factors within three time periods of wheat growth: 2, 6 and 10 weeks prior to embryo sampling were analysed by biplot analysis. The tissue culture traits were influenced at very different degrees by climatic factors: from 16.8% (RC) to 69.8% (CF). Donor plant environment with high temperatures and low rainfalls reduced (p lt 0.05) the tissue culture performance of wheat genotypes. Callus formation was most sensitive to the temperature based factors. The environmental conditions between flowering and the medium milk stage were the most important for CF, while RC and PPE were not particularly related to any period.", publisher = "Univ Sao Paolo, Cerquera Cesar", journal = "Scientia Agricola", title = "Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant", volume = "67", number = "3", pages = "295-300", doi = "10.1590/S0103-90162010000300007" }
Dodig, D., Zorić, M., Lalević, B., Šurlan-Momirović, G., Mitić, N., Nikolić, R.,& King, S. R.. (2010). Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant. in Scientia Agricola Univ Sao Paolo, Cerquera Cesar., 67(3), 295-300. https://doi.org/10.1590/S0103-90162010000300007
Dodig D, Zorić M, Lalević B, Šurlan-Momirović G, Mitić N, Nikolić R, King SR. Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant. in Scientia Agricola. 2010;67(3):295-300. doi:10.1590/S0103-90162010000300007 .
Dodig, Dejan, Zorić, Miroslav, Lalević, Blazo, Šurlan-Momirović, Gordana, Mitić, Nevena, Nikolić, Radomirka, King, Stephen R., "Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant" in Scientia Agricola, 67, no. 3 (2010):295-300, https://doi.org/10.1590/S0103-90162010000300007 . .