Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes
Само за регистроване кориснике
2009
Аутори
Mitić, NevenaNikolić, Radomirka
Ninković, Slavica
Vinterhalter, D.
Vinterhalter, Branka
Dodig, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12-15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as l...arge as the effect of the genotype, which should be taken into account when planning transformation work with wheat.
Кључне речи:
Triticum aestivum / immature embryo / growth conditions / tissue culture response / plant regenerationИзвор:
Russian Journal of Plant Physiology, 2009, 56, 4, 540-545Издавач:
- Maik Nauka/Interperiodica/Springer, New York
Финансирање / пројекти:
- Регулација морфогенетских процеса и секундарног метаболизма и генетичке трансформације биљака у култури in vitro (RS-MESTD-MPN2006-2010-143026)
DOI: 10.1134/S1021443709040141
ISSN: 1021-4437
WoS: 000267487700014
Scopus: 2-s2.0-70349658658
Институција/група
MRIZPTY - JOUR AU - Mitić, Nevena AU - Nikolić, Radomirka AU - Ninković, Slavica AU - Vinterhalter, D. AU - Vinterhalter, Branka AU - Dodig, Dejan PY - 2009 UR - http://rik.mrizp.rs/handle/123456789/273 AB - The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12-15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as large as the effect of the genotype, which should be taken into account when planning transformation work with wheat. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Russian Journal of Plant Physiology T1 - Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes VL - 56 IS - 4 SP - 540 EP - 545 DO - 10.1134/S1021443709040141 ER -
@article{ author = "Mitić, Nevena and Nikolić, Radomirka and Ninković, Slavica and Vinterhalter, D. and Vinterhalter, Branka and Dodig, Dejan", year = "2009", abstract = "The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12-15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as large as the effect of the genotype, which should be taken into account when planning transformation work with wheat.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Russian Journal of Plant Physiology", title = "Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes", volume = "56", number = "4", pages = "540-545", doi = "10.1134/S1021443709040141" }
Mitić, N., Nikolić, R., Ninković, S., Vinterhalter, D., Vinterhalter, B.,& Dodig, D.. (2009). Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes. in Russian Journal of Plant Physiology Maik Nauka/Interperiodica/Springer, New York., 56(4), 540-545. https://doi.org/10.1134/S1021443709040141
Mitić N, Nikolić R, Ninković S, Vinterhalter D, Vinterhalter B, Dodig D. Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes. in Russian Journal of Plant Physiology. 2009;56(4):540-545. doi:10.1134/S1021443709040141 .
Mitić, Nevena, Nikolić, Radomirka, Ninković, Slavica, Vinterhalter, D., Vinterhalter, Branka, Dodig, Dejan, "Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes" in Russian Journal of Plant Physiology, 56, no. 4 (2009):540-545, https://doi.org/10.1134/S1021443709040141 . .