The quantitative determination of antioxidative-protective and some storage substances of seeds and leaves: Modification of methodological approach
Kvantitativno određivanje antioksidativno-protektivnih i pojedinih rezervnih supstanci semena i listova - modifikacija metodološkog pristupa
Апстракт
The modification was done with a purpose to find the integral medium for extraction of some organic and nonorganic substances responsible for seed germination and plant growth: phytine, PO4 ion, as well as, stress protecting substances of a different origin: total soluble phenols and thiols in seeds and leaves. This modification implied the selection of the extraction medium suitable for the quantitative isolation of substances from the common extracts of the initial material (seed, leaf). It was shown that highly diluted 0.15% tri-sodium-citrate can be used as a universal extraction medium for phytine, ortho-phosphate ion, soluble total thiols and total phenols. This medium allowed the efficient extraction of phytine and phosphate from degermed milled maize seeds. Also, it is sensitive enough to make difference between their content in young and mature maize leaves. Two weighted samples of 0.1-0.5 g, from the average sample of 25 seeds or seedlings, five young plants, as well as, two ...leaves (above and bellow maize cob) from three plants in the tasseling stage, with the addition of 8-15 ml of the above mentioned solution, provided a spectrophotometrical determination of these substances in 2-3 replicates. The procedure was developed to be suitable for the introduction and breeding, including a unique minimum average sample for all observed parameters. The application of precise and rapid spectrophotometrical methods, modified for large sample series, provides the reduction in the number of replicates to two (the statistical minimum), hence there is enough space for the introduction of the other desirable analyses.
U ovom radu izvršena je modifikacija metoda za određivanje organskih i neorganskih supstanci odgovornih za klijanje semena i rast biljaka: fitin i slobodni PO4 jon, kao i antistresnih faktora različitog porekla: ukupnih rastvorljivih tiola i fenola, u semenu i biljci, a koja se sastojala u izboru ekstrakcionog sredstva pogodnog za kvantitativno izolovanje navedenih supstanci iz zajedničkog ekstrakta početnog materijala (seme, list). Pokazalo se da je 0,15% tri-natrijum-cirtat podjednako efikasan za ukupne toile, fitin, slobodni PO4 jon i ukupne fenole. Dalje, dve odvagane supstance od 0,1 do 0,5 g iz prosečnog uzorka: 25 semena, odnosno klijanaca; pet mladih biljaka sa 5-7 listova, kao i dva lista (listovi ispod i iznad klipa) sa po tri biljke u fazi metličanja, sa 8 do 15 ml datog rastvora omogućava spektrofotometrijsko određivanje navedenih supstanci u dva, odnosno tri ponavljanja. Procedura je razrađena za potrebe introdukcije i selekcije, gde se raspolaže sa minimalnim prosečnim uz...orkom koji se može upotrebiti za analize. U analitičkom smislu problem predstavlja mala masa uzorka kod sitnih semena i klijanaca, što zahteva minimalnu odvaganu supstancu i broj ponavljanja. Primena preciznih i brzih spektrofotometrijskih metoda, modifikovanih za velike serije, omogućava smanjenje broja ponavljanja na dva (statistički minimum), tako da preostaje dosta prostora (ml ekstrakta, g polaznog uzorka) da se uvedu i druge željene analize.
Кључне речи:
minimalni prosečni uzorak / mogućnost proširenja analiza na druge poželjne faktore / precizne i brze spektrofotometrijske metode / zajedničko ekstrakciono sredstvoИзвор:
Journal of Scientific Agricultural Research, 2009, 70, 1, 13-20Издавач:
- Savez poljoprivrednih inženjera i tehničara, Beograd
Институција/група
MRIZPTY - JOUR AU - Sredojević, Slobodanka AU - Dragičević, Vesna PY - 2009 UR - http://rik.mrizp.rs/handle/123456789/262 AB - The modification was done with a purpose to find the integral medium for extraction of some organic and nonorganic substances responsible for seed germination and plant growth: phytine, PO4 ion, as well as, stress protecting substances of a different origin: total soluble phenols and thiols in seeds and leaves. This modification implied the selection of the extraction medium suitable for the quantitative isolation of substances from the common extracts of the initial material (seed, leaf). It was shown that highly diluted 0.15% tri-sodium-citrate can be used as a universal extraction medium for phytine, ortho-phosphate ion, soluble total thiols and total phenols. This medium allowed the efficient extraction of phytine and phosphate from degermed milled maize seeds. Also, it is sensitive enough to make difference between their content in young and mature maize leaves. Two weighted samples of 0.1-0.5 g, from the average sample of 25 seeds or seedlings, five young plants, as well as, two leaves (above and bellow maize cob) from three plants in the tasseling stage, with the addition of 8-15 ml of the above mentioned solution, provided a spectrophotometrical determination of these substances in 2-3 replicates. The procedure was developed to be suitable for the introduction and breeding, including a unique minimum average sample for all observed parameters. The application of precise and rapid spectrophotometrical methods, modified for large sample series, provides the reduction in the number of replicates to two (the statistical minimum), hence there is enough space for the introduction of the other desirable analyses. AB - U ovom radu izvršena je modifikacija metoda za određivanje organskih i neorganskih supstanci odgovornih za klijanje semena i rast biljaka: fitin i slobodni PO4 jon, kao i antistresnih faktora različitog porekla: ukupnih rastvorljivih tiola i fenola, u semenu i biljci, a koja se sastojala u izboru ekstrakcionog sredstva pogodnog za kvantitativno izolovanje navedenih supstanci iz zajedničkog ekstrakta početnog materijala (seme, list). Pokazalo se da je 0,15% tri-natrijum-cirtat podjednako efikasan za ukupne toile, fitin, slobodni PO4 jon i ukupne fenole. Dalje, dve odvagane supstance od 0,1 do 0,5 g iz prosečnog uzorka: 25 semena, odnosno klijanaca; pet mladih biljaka sa 5-7 listova, kao i dva lista (listovi ispod i iznad klipa) sa po tri biljke u fazi metličanja, sa 8 do 15 ml datog rastvora omogućava spektrofotometrijsko određivanje navedenih supstanci u dva, odnosno tri ponavljanja. Procedura je razrađena za potrebe introdukcije i selekcije, gde se raspolaže sa minimalnim prosečnim uzorkom koji se može upotrebiti za analize. U analitičkom smislu problem predstavlja mala masa uzorka kod sitnih semena i klijanaca, što zahteva minimalnu odvaganu supstancu i broj ponavljanja. Primena preciznih i brzih spektrofotometrijskih metoda, modifikovanih za velike serije, omogućava smanjenje broja ponavljanja na dva (statistički minimum), tako da preostaje dosta prostora (ml ekstrakta, g polaznog uzorka) da se uvedu i druge željene analize. PB - Savez poljoprivrednih inženjera i tehničara, Beograd T2 - Journal of Scientific Agricultural Research T1 - The quantitative determination of antioxidative-protective and some storage substances of seeds and leaves: Modification of methodological approach T1 - Kvantitativno određivanje antioksidativno-protektivnih i pojedinih rezervnih supstanci semena i listova - modifikacija metodološkog pristupa VL - 70 IS - 1 SP - 13 EP - 20 UR - https://hdl.handle.net/21.15107/rcub_rik_262 ER -
@article{ author = "Sredojević, Slobodanka and Dragičević, Vesna", year = "2009", abstract = "The modification was done with a purpose to find the integral medium for extraction of some organic and nonorganic substances responsible for seed germination and plant growth: phytine, PO4 ion, as well as, stress protecting substances of a different origin: total soluble phenols and thiols in seeds and leaves. This modification implied the selection of the extraction medium suitable for the quantitative isolation of substances from the common extracts of the initial material (seed, leaf). It was shown that highly diluted 0.15% tri-sodium-citrate can be used as a universal extraction medium for phytine, ortho-phosphate ion, soluble total thiols and total phenols. This medium allowed the efficient extraction of phytine and phosphate from degermed milled maize seeds. Also, it is sensitive enough to make difference between their content in young and mature maize leaves. Two weighted samples of 0.1-0.5 g, from the average sample of 25 seeds or seedlings, five young plants, as well as, two leaves (above and bellow maize cob) from three plants in the tasseling stage, with the addition of 8-15 ml of the above mentioned solution, provided a spectrophotometrical determination of these substances in 2-3 replicates. The procedure was developed to be suitable for the introduction and breeding, including a unique minimum average sample for all observed parameters. The application of precise and rapid spectrophotometrical methods, modified for large sample series, provides the reduction in the number of replicates to two (the statistical minimum), hence there is enough space for the introduction of the other desirable analyses., U ovom radu izvršena je modifikacija metoda za određivanje organskih i neorganskih supstanci odgovornih za klijanje semena i rast biljaka: fitin i slobodni PO4 jon, kao i antistresnih faktora različitog porekla: ukupnih rastvorljivih tiola i fenola, u semenu i biljci, a koja se sastojala u izboru ekstrakcionog sredstva pogodnog za kvantitativno izolovanje navedenih supstanci iz zajedničkog ekstrakta početnog materijala (seme, list). Pokazalo se da je 0,15% tri-natrijum-cirtat podjednako efikasan za ukupne toile, fitin, slobodni PO4 jon i ukupne fenole. Dalje, dve odvagane supstance od 0,1 do 0,5 g iz prosečnog uzorka: 25 semena, odnosno klijanaca; pet mladih biljaka sa 5-7 listova, kao i dva lista (listovi ispod i iznad klipa) sa po tri biljke u fazi metličanja, sa 8 do 15 ml datog rastvora omogućava spektrofotometrijsko određivanje navedenih supstanci u dva, odnosno tri ponavljanja. Procedura je razrađena za potrebe introdukcije i selekcije, gde se raspolaže sa minimalnim prosečnim uzorkom koji se može upotrebiti za analize. U analitičkom smislu problem predstavlja mala masa uzorka kod sitnih semena i klijanaca, što zahteva minimalnu odvaganu supstancu i broj ponavljanja. Primena preciznih i brzih spektrofotometrijskih metoda, modifikovanih za velike serije, omogućava smanjenje broja ponavljanja na dva (statistički minimum), tako da preostaje dosta prostora (ml ekstrakta, g polaznog uzorka) da se uvedu i druge željene analize.", publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd", journal = "Journal of Scientific Agricultural Research", title = "The quantitative determination of antioxidative-protective and some storage substances of seeds and leaves: Modification of methodological approach, Kvantitativno određivanje antioksidativno-protektivnih i pojedinih rezervnih supstanci semena i listova - modifikacija metodološkog pristupa", volume = "70", number = "1", pages = "13-20", url = "https://hdl.handle.net/21.15107/rcub_rik_262" }
Sredojević, S.,& Dragičević, V.. (2009). The quantitative determination of antioxidative-protective and some storage substances of seeds and leaves: Modification of methodological approach. in Journal of Scientific Agricultural Research Savez poljoprivrednih inženjera i tehničara, Beograd., 70(1), 13-20. https://hdl.handle.net/21.15107/rcub_rik_262
Sredojević S, Dragičević V. The quantitative determination of antioxidative-protective and some storage substances of seeds and leaves: Modification of methodological approach. in Journal of Scientific Agricultural Research. 2009;70(1):13-20. https://hdl.handle.net/21.15107/rcub_rik_262 .
Sredojević, Slobodanka, Dragičević, Vesna, "The quantitative determination of antioxidative-protective and some storage substances of seeds and leaves: Modification of methodological approach" in Journal of Scientific Agricultural Research, 70, no. 1 (2009):13-20, https://hdl.handle.net/21.15107/rcub_rik_262 .