Polymorphism of protein markers in Serbian spruce half-sib lines

Abstract

This paper present the results of determination of the degree of genetic polymorphism of Serbian spruce half-sib lines belonging to different varieties: var .'semidihotomy', var.'serbica' and var.'nana', incorporated in the seedling seed orchard, by the application of protein markers. The analysis in the salts of soluble proteins of the endosperms of 12 Serbian spruce genotypes was performed by separation - electrophoresis on the polyacrylamide gel using Leammli (1970) method. The obtained electrophoregrams were the base for the calculation of the coefficient of similarity, by Sheen (1972) formula, both between individuals within the lines and between the lines. The genetic distance of the analyzed material was assessed by cluster analysis. The study results show that phenotypic variability between the analyzed varieties of Serbian spruce is genetically determined.

U radu su prikazani rezultati utvrđivanja stepena genetskog polimorfizma linija polusrodnika, ugrađenih u generativnu semensku plantažu omorike koji pripadaju različitim varijetetima omorike: var. 'semidihotomy', var.'serbica' i var. 'nana', primenom proteinskih markera. Analiza u solima rastvorljivih proteina, semena 12 genotipova omorike, obavljena je razdvajanjem-elektroforezom na poliakrilamidnom gelu metodom Leammli (1970). Dobijeni elektroforegrami poslužili su kao osnova za izračunavanje koeficijenta sličnosti po formuli Sheen-a (1972), kako između individua unutar samih linija, tako i među linijma. Genetska bliskost odnosno udaljenost između analiziranog materijala utvrđena je primenom Klaster analize.