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Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain

Procena genetičke diferencijacije jele (Abies alba Mill.) sa Javora pomoću SSR markera

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2019
2019-Genetika_0534-00121903103P.pdf (403.2Kb)
Authors
Popović, Vladan
Lučić, Aleksandar
Rakonjac, Ljubinko
Milovanović, Jelena
Mladenović-Drinić, Snežana
Ristić, Danijela
Article (Published version)
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Abstract
The process of plant breeding and conservation of gene pool among other things depends on the knowledge of the level of genetic differentiation. The aim of research in this paper was to determine the genetic differentiation of silver fir (Abies albaMill.) populations of regular type and atypical genotypes with pyramidal crown that can be found on Javor mountain, at the site Ogorijevac. The genetic differentiation of silver fir was determined using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave 29 alleles, while the average number of alleles was 3.2. The primer NFH15 gave the smallest number of alleles (two), while the primer SF78 gave the greatest number of alleles (five). Dice coefficient of the genetic similarity was used to obtain a dendrogram by UPMGA analysis using NTSYSpc statistical program. The genetic similarity recorded among the individuals P1 and P2 was the largest (0.89), while the populations VI a...nd individual P2 showed the lowest similarity (0.61).Based on the cluster analysis it canbe concluded that the studied populations and genotypes of silver fir with different types of crown are clearly differentiated. The basic insight into the level of the genetic diversity of the natural populations of silver fir with the various types of crown has been provided using selected SSR markers. The obtained results can be used for creating further strategy for the conservation of the available gene pool and the regeneration of silver fir forests in Serbia.

Proces oplemenjivanja biljaka i konzervacije genofonda između ostalog, zavisi i od poznavanja stepena genetičke diferencijacije. Cilj istraživanja u ovom radu bio je da se utvrdi genetička diferencijacija populacija jele (Abies alba Mill.)normalanog tipa i atipičnih genotipova piramidalanog tipa krošnje, koje se javljaju na planini Javor, na lokalitetu Ogorijevac. Genetička sličnost odnosno različitost jele određena je upotrebom SSR (Simple Sequence Repeat) markera. Devet SSR pari prajmera dalo je ukupno 29 alela, dok je prosečan broj alela bio 3,2. Prajmer NFH15 dao je najmanji broj alela (dva), dok je prajmer SF78 bio sa najvećim brojem alela (pet). Najviša vrednost koeficijenta genetičke sličnosti utvrđena je između genotipova P1 i P2 (0,89), dok je najniža vrednost koeficijenta genetičke sličnosti utvrđena između populacije VI i genotipa P2 (0,61).Koeficijenti genetičke sličnosti po Dice, upotrebljeni su za dobijanje dendrograma pomoću UPMGA anal...ize, koristeći NTSYSpc statistički program. Na osnovu klaster analize može se zaključiti da su istraživane populacije i genotipovi jele sa različitim tipom krošnje jasno izdiferencirani. Upotrebom izabranih SSR markera dat je osnovni uvid u nivo genetičke raznovrsnosti prirodnih populacija jele različitog tipa krošnje. Dobijeni rezultati mogu poslužiti u budućoj strategiji na konzervaciji raspoloživog genofonda i obnavljanju jelovih šuma u Srbiji.

Keywords:
Abies alba Mill. / SSR (Simple Sequence Repeat) markers / population / differentiation
Source:
Genetika, 2019, 51, 3, 1103-1112
Publisher:
  • Beograd : Društvo genetičara Srbije
  • Belgrade : Serbian Genetics Society
Funding / projects:
  • The development of technological procedures in forestry with a view to an optimum forest cover realisation (RS-31070)

DOI: 10.2298/GENSR1903103P

ISSN: 0534-0012

WoS: 000509981300023

Scopus: 2-s2.0-85081286396
[ Google Scholar ]
1
1
URI
http://rik.mrizp.rs/handle/123456789/799
Collections
  • Радови истраживача / Researchers' publications
Institution/Community
MRIZP
TY  - JOUR
AU  - Popović, Vladan
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Milovanović, Jelena
AU  - Mladenović-Drinić, Snežana
AU  - Ristić, Danijela
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/799
AB  - The process of plant breeding and conservation of gene pool among other things depends on the knowledge of the level of genetic differentiation. The aim of research in this paper was to determine the genetic differentiation of silver fir (Abies albaMill.) populations of regular  type  and  atypical  genotypes  with  pyramidal  crown  that  can  be  found  on  Javor mountain, at  the  site Ogorijevac. The  genetic  differentiation of silver fir  was determined using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave 29 alleles, while  the  average  number  of  alleles  was  3.2.  The  primer  NFH15  gave  the  smallest number of alleles (two), while the primer SF78 gave the greatest number of alleles (five). Dice  coefficient  of  the  genetic  similarity  was  used  to  obtain  a  dendrogram  by  UPMGA analysis  using  NTSYSpc  statistical  program.  The  genetic  similarity  recorded  among  the individuals P1 and P2 was the largest (0.89), while the populations VI and individual P2 showed the lowest similarity (0.61).Based on the cluster analysis it canbe concluded that the  studied populations and genotypes of silver  fir  with different types of crown are  clearly differentiated. The  basic insight  into  the  level  of  the  genetic  diversity  of  the  natural  populations  of  silver  fir  with the  various types of crown has been provided using selected SSR markers. The obtained results can be used for creating further strategy for the conservation of the available gene pool and the regeneration of silver fir forests in Serbia.
AB  - Proces oplemenjivanja biljaka i konzervacije genofonda između ostalog, zavisi i od poznavanja stepena  genetičke  diferencijacije.  Cilj  istraživanja  u  ovom  radu  bio  je  da  se  utvrdi  genetička diferencijacija   populacija   jele   (Abies   alba Mill.)normalanog  tipa  i  atipičnih  genotipova piramidalanog tipa krošnje, koje se javljaju na planini Javor, na lokalitetu Ogorijevac. Genetička sličnost odnosno različitost jele određena je upotrebom SSR (Simple Sequence Repeat) markera. Devet SSR pari prajmera dalo je ukupno 29 alela, dok je prosečan broj alela bio 3,2. Prajmer NFH15 dao je najmanji broj alela (dva), dok je prajmer SF78 bio sa najvećim brojem alela (pet). Najviša vrednost koeficijenta genetičke sličnosti utvrđena je između genotipova P1 i P2 (0,89), dok je najniža vrednost koeficijenta genetičke sličnosti utvrđena između populacije VI i genotipa P2 (0,61).Koeficijenti  genetičke  sličnosti  po  Dice,  upotrebljeni  su  za  dobijanje  dendrograma  pomoću UPMGA  analize,  koristeći  NTSYSpc  statistički  program.  Na  osnovu  klaster  analize  može  se zaključiti  da  su  istraživane  populacije  i  genotipovi  jele  sa  različitim  tipom  krošnje  jasno izdiferencirani.  Upotrebom  izabranih  SSR  markera  dat  je  osnovni  uvid  u  nivo  genetičke raznovrsnosti prirodnih populacija jele različitog tipa krošnje. Dobijeni rezultati mogu poslužiti u budućoj strategiji na konzervaciji raspoloživog genofonda i obnavljanju jelovih šuma u Srbiji.
PB  - Beograd : Društvo genetičara Srbije
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain
T1  - Procena genetičke diferencijacije jele (Abies alba Mill.) sa Javora pomoću SSR markera
VL  - 51
IS  - 3
SP  - 1103
EP  - 1112
DO  - 10.2298/GENSR1903103P
ER  - 
@article{
author = "Popović, Vladan and Lučić, Aleksandar and Rakonjac, Ljubinko and Milovanović, Jelena and Mladenović-Drinić, Snežana and Ristić, Danijela",
year = "2019",
abstract = "The process of plant breeding and conservation of gene pool among other things depends on the knowledge of the level of genetic differentiation. The aim of research in this paper was to determine the genetic differentiation of silver fir (Abies albaMill.) populations of regular  type  and  atypical  genotypes  with  pyramidal  crown  that  can  be  found  on  Javor mountain, at  the  site Ogorijevac. The  genetic  differentiation of silver fir  was determined using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave 29 alleles, while  the  average  number  of  alleles  was  3.2.  The  primer  NFH15  gave  the  smallest number of alleles (two), while the primer SF78 gave the greatest number of alleles (five). Dice  coefficient  of  the  genetic  similarity  was  used  to  obtain  a  dendrogram  by  UPMGA analysis  using  NTSYSpc  statistical  program.  The  genetic  similarity  recorded  among  the individuals P1 and P2 was the largest (0.89), while the populations VI and individual P2 showed the lowest similarity (0.61).Based on the cluster analysis it canbe concluded that the  studied populations and genotypes of silver  fir  with different types of crown are  clearly differentiated. The  basic insight  into  the  level  of  the  genetic  diversity  of  the  natural  populations  of  silver  fir  with the  various types of crown has been provided using selected SSR markers. The obtained results can be used for creating further strategy for the conservation of the available gene pool and the regeneration of silver fir forests in Serbia., Proces oplemenjivanja biljaka i konzervacije genofonda između ostalog, zavisi i od poznavanja stepena  genetičke  diferencijacije.  Cilj  istraživanja  u  ovom  radu  bio  je  da  se  utvrdi  genetička diferencijacija   populacija   jele   (Abies   alba Mill.)normalanog  tipa  i  atipičnih  genotipova piramidalanog tipa krošnje, koje se javljaju na planini Javor, na lokalitetu Ogorijevac. Genetička sličnost odnosno različitost jele određena je upotrebom SSR (Simple Sequence Repeat) markera. Devet SSR pari prajmera dalo je ukupno 29 alela, dok je prosečan broj alela bio 3,2. Prajmer NFH15 dao je najmanji broj alela (dva), dok je prajmer SF78 bio sa najvećim brojem alela (pet). Najviša vrednost koeficijenta genetičke sličnosti utvrđena je između genotipova P1 i P2 (0,89), dok je najniža vrednost koeficijenta genetičke sličnosti utvrđena između populacije VI i genotipa P2 (0,61).Koeficijenti  genetičke  sličnosti  po  Dice,  upotrebljeni  su  za  dobijanje  dendrograma  pomoću UPMGA  analize,  koristeći  NTSYSpc  statistički  program.  Na  osnovu  klaster  analize  može  se zaključiti  da  su  istraživane  populacije  i  genotipovi  jele  sa  različitim  tipom  krošnje  jasno izdiferencirani.  Upotrebom  izabranih  SSR  markera  dat  je  osnovni  uvid  u  nivo  genetičke raznovrsnosti prirodnih populacija jele različitog tipa krošnje. Dobijeni rezultati mogu poslužiti u budućoj strategiji na konzervaciji raspoloživog genofonda i obnavljanju jelovih šuma u Srbiji.",
publisher = "Beograd : Društvo genetičara Srbije, Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain, Procena genetičke diferencijacije jele (Abies alba Mill.) sa Javora pomoću SSR markera",
volume = "51",
number = "3",
pages = "1103-1112",
doi = "10.2298/GENSR1903103P"
}
Popović, V., Lučić, A., Rakonjac, L., Milovanović, J., Mladenović-Drinić, S.,& Ristić, D.. (2019). Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain. in Genetika
Beograd : Društvo genetičara Srbije., 51(3), 1103-1112.
https://doi.org/10.2298/GENSR1903103P
Popović V, Lučić A, Rakonjac L, Milovanović J, Mladenović-Drinić S, Ristić D. Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain. in Genetika. 2019;51(3):1103-1112.
doi:10.2298/GENSR1903103P .
Popović, Vladan, Lučić, Aleksandar, Rakonjac, Ljubinko, Milovanović, Jelena, Mladenović-Drinić, Snežana, Ristić, Danijela, "Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain" in Genetika, 51, no. 3 (2019):1103-1112,
https://doi.org/10.2298/GENSR1903103P . .

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