Determination of free phenolic acids from leaves within different colored maize
2017
Authors
Mesarović, JelenaDragičević, Vesna
Mladenović Drinić, Snežana
Ristić, Danijela
Kravić, Natalija
Article (Published version)
Metadata
Show full item recordAbstract
Along with other plant parts, maize leaves are widely used for making fermented food for cattle, known as silage. Since there have only been a few reports on studies concerning the extraction and determination of phenolic acids from maize leaves, the main goal of this investigation was to evaluate the content of free phenolic acids in the leaves of fifteen different maize inbred lines. Reverse-phase, high performance liquid chromatography (RP-HPLC), with a photodiode array detector (DAD), was performed. Under the optimized chromatographic conditions, referring to short time of sample preparation, small quantities of solvent and direct injection of the extract into HPLC, phenolic acids (i.e., gallic, protocatechuic, caffeic, p-coumaric and ferulic acid) were successfully separated in less than 25 min, indicating that the method could be applied for routine analysis. The efficiency and validation of the method was evaluated by measuring the rate parameters: linearity, limit of detection ...and quantification, accuracy and precision. The obtained results showed that the most abundant free phenolic acid was p-coumaric acid (23.57 mu g g(-1) dry weight), followed by ferulic and caffeic acids (21.27 and 20.78 mu g g(-1) dry weight, respectively). Principal Component Analysis (PCA) revealed the existence of a link.
Keywords:
HPLC-DAD / method validation / phenolic acids / cornSource:
Journal of the Serbian Chemical Society, 2017, 82, 1, 63-72Publisher:
- Srpsko hemijsko društvo, Beograd
Funding / projects:
- Improvment of maize and soybean traits by molecular and conventional breeding (RS-MESTD-Technological Development (TD or TR)-31068)
- Exploitation of maize diversity to improve grain quality and drought tolerance (RS-MESTD-Technological Development (TD or TR)-31028)
DOI: 10.2298/JSC160512104M
ISSN: 0352-5139