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Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover

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2016
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Autori
Vasić, Tanja
Terzić, Dragan
Milenković, Jasmina
Marković, Jordan
Krnjaja, Vesna
Jevremović, Darko
Stanković, Slavica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geog...raphical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.

Ključne reči:
RFLP / VCGs / M. sativa / T. pratense / C. destructivum
Izvor:
Genetika, 2016, 48, 1, 187-198
Izdavač:
  • Društvo genetičara Srbije, Beograd
Projekti:
  • Poboljšanje genetičkog potencijala i tehnologija proizvodnje krmnog bilja u funkciji održivog razvoja stočarstva (RS-31057)

DOI: 10.2298/GENSR1601187V

ISSN: 0534-0012

WoS: 000376744800016

Scopus: 2-s2.0-84971219286
[ Google Scholar ]
URI
http://rik.mrizp.rs/handle/123456789/650
Kolekcije
  • Радови истраживача / Researchers' publications
Institucija
MRIZP

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