A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geog...raphical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
Keywords:
RFLP / VCGs / M. sativa / T. pratense / C. destructivum
TY - JOUR
AU - Vasić, Tanja
AU - Terzić, Dragan
AU - Milenković, Jasmina
AU - Marković, Jordan
AU - Krnjaja, Vesna
AU - Jevremović, Darko
AU - Stanković, Slavica
PY - 2016
UR - http://rik.mrizp.rs/handle/123456789/650
AB - A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
PB - Društvo genetičara Srbije, Beograd
T2 - Genetika
T1 - Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover
VL - 48
IS - 1
SP - 187
EP - 198
DO - 10.2298/GENSR1601187V
UR - conv_930
ER -
@article{
author = "Vasić, Tanja and Terzić, Dragan and Milenković, Jasmina and Marković, Jordan and Krnjaja, Vesna and Jevremović, Darko and Stanković, Slavica",
year = "2016",
abstract = "A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover",
volume = "48",
number = "1",
pages = "187-198",
doi = "10.2298/GENSR1601187V",
url = "conv_930"
}
Vasić, T., Terzić, D., Milenković, J., Marković, J., Krnjaja, V., Jevremović, D.,& Stanković, S.. (2016). Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika
Društvo genetičara Srbije, Beograd., 48(1), 187-198.
https://doi.org/10.2298/GENSR1601187V
conv_930
Vasić T, Terzić D, Milenković J, Marković J, Krnjaja V, Jevremović D, Stanković S. Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika. 2016;48(1):187-198.
doi:10.2298/GENSR1601187V
conv_930 .
Vasić, Tanja, Terzić, Dragan, Milenković, Jasmina, Marković, Jordan, Krnjaja, Vesna, Jevremović, Darko, Stanković, Slavica, "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover" in Genetika, 48, no. 1 (2016):187-198,
https://doi.org/10.2298/GENSR1601187V .,
conv_930 .
