Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover

2016
Authors
Vasić, Tanja
Terzić, Dragan
Milenković, Jasmina
Marković, Jordan
Krnjaja, Vesna

Jevremović, Darko
Stanković, Slavica

Article (Published version)
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A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geog...raphical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
Keywords:
RFLP / VCGs / M. sativa / T. pratense / C. destructivumSource:
Genetika, 2016, 48, 1, 187-198Publisher:
- Društvo genetičara Srbije, Beograd
Funding / projects:
DOI: 10.2298/GENSR1601187V
ISSN: 0534-0012
WoS: 000376744800016
Scopus: 2-s2.0-84971219286
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MRIZPTY - JOUR AU - Vasić, Tanja AU - Terzić, Dragan AU - Milenković, Jasmina AU - Marković, Jordan AU - Krnjaja, Vesna AU - Jevremović, Darko AU - Stanković, Slavica PY - 2016 UR - http://rik.mrizp.rs/handle/123456789/650 AB - A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover VL - 48 IS - 1 SP - 187 EP - 198 DO - 10.2298/GENSR1601187V UR - conv_930 ER -
@article{ author = "Vasić, Tanja and Terzić, Dragan and Milenković, Jasmina and Marković, Jordan and Krnjaja, Vesna and Jevremović, Darko and Stanković, Slavica", year = "2016", abstract = "A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover", volume = "48", number = "1", pages = "187-198", doi = "10.2298/GENSR1601187V", url = "conv_930" }
Vasić, T., Terzić, D., Milenković, J., Marković, J., Krnjaja, V., Jevremović, D.,& Stanković, S.. (2016). Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika Društvo genetičara Srbije, Beograd., 48(1), 187-198. https://doi.org/10.2298/GENSR1601187V conv_930
Vasić T, Terzić D, Milenković J, Marković J, Krnjaja V, Jevremović D, Stanković S. Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika. 2016;48(1):187-198. doi:10.2298/GENSR1601187V conv_930 .
Vasić, Tanja, Terzić, Dragan, Milenković, Jasmina, Marković, Jordan, Krnjaja, Vesna, Jevremović, Darko, Stanković, Slavica, "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover" in Genetika, 48, no. 1 (2016):187-198, https://doi.org/10.2298/GENSR1601187V ., conv_930 .