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Plasma-membrane-bound malate dehydrogenase activity in maize roots

Authorized Users Only
1999
Authors
Hadži-Tašković-Šukalović, Vesna
Vuletić, Mirjana
Ignjatović-Micić, Dragana
Vučinić, Željko
Article (Published version)
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Abstract
Plasma membranes were isolated and purified from 14-day-old maize roots (Zea mays L.) by two-phase partitioning at a 6.5% polymer concentration, and compared to isolated mitochondria, microsomes, and soluble fraction. Marker enzyme analysis demonstrated that the plasma membranes were devoid of cytoplasmic, mitochondrial, tonoplast, and endoplasmic-reticulum contaminations. Isolated plasma membranes exhibited malate dehydrogenase activity, catalyzing NADH-dependent reduction of oxaloacetate as well as NAD + -dependent malate oxidation. Malate dehydrogenase activity was resistant to osmotic shock, freeze-thaw treatment, and salt washing and stimulated by solubilization with Triton X-100, indicating that the enzyme is tightly bound to the plasma membrane. Malate dehydrogenase activity was highly specific to NAD + and NADH. The enzyme exhibited a high degree of latency in both right-side-out (80%) and inside-out (70%) vesicle preparations. Kinetic and regulatory properties with ATP and P(i...), as well as pH dependence of plasma-membrane-bound malate dehydrogenase were different from mitochondrial and soluble malate dehydrogenases. Starch gel electrophoresis revealed a characteristic isozyme form present in the plasma membrane isolate, but not present in the soluble, mitochondrial, and microsomal fractions. The results presented show that purified plasma membranes isolated from maize roots contain a tightly associated malate dehydrogenase, having properties different from mitochondrial and soluble malate dehydrogenases.

Keywords:
S -- Ferricyanide reductase / Isozyme form / Malate dehydrogenase / Oxaloacetate / Plasma membrane / Root / Zea mays L.
Source:
Protoplasma, 1999, 207, 3-4, 203-212

ISSN: 0033-183X

Scopus: 2-s2.0-0032783659
[ Google Scholar ]
14
URI
http://rik.mrizp.rs/handle/123456789/6
Collections
  • Радови истраживача / Researchers' publications
Institution/Community
MRIZP
TY  - JOUR
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vuletić, Mirjana
AU  - Ignjatović-Micić, Dragana
AU  - Vučinić, Željko
PY  - 1999
UR  - http://rik.mrizp.rs/handle/123456789/6
AB  - Plasma membranes were isolated and purified from 14-day-old maize roots (Zea mays L.) by two-phase partitioning at a 6.5% polymer concentration, and compared to isolated mitochondria, microsomes, and soluble fraction. Marker enzyme analysis demonstrated that the plasma membranes were devoid of cytoplasmic, mitochondrial, tonoplast, and endoplasmic-reticulum contaminations. Isolated plasma membranes exhibited malate dehydrogenase activity, catalyzing NADH-dependent reduction of oxaloacetate as well as NAD + -dependent malate oxidation. Malate dehydrogenase activity was resistant to osmotic shock, freeze-thaw treatment, and salt washing and stimulated by solubilization with Triton X-100, indicating that the enzyme is tightly bound to the plasma membrane. Malate dehydrogenase activity was highly specific to NAD + and NADH. The enzyme exhibited a high degree of latency in both right-side-out (80%) and inside-out (70%) vesicle preparations. Kinetic and regulatory properties with ATP and P(i), as well as pH dependence of plasma-membrane-bound malate dehydrogenase were different from mitochondrial and soluble malate dehydrogenases. Starch gel electrophoresis revealed a characteristic isozyme form present in the plasma membrane isolate, but not present in the soluble, mitochondrial, and microsomal fractions. The results presented show that purified plasma membranes isolated from maize roots contain a tightly associated malate dehydrogenase, having properties different from mitochondrial and soluble malate dehydrogenases.
T2  - Protoplasma
T1  - Plasma-membrane-bound malate dehydrogenase activity in maize roots
VL  - 207
IS  - 3-4
SP  - 203
EP  - 212
UR  - conv_1071
ER  - 
@article{
author = "Hadži-Tašković-Šukalović, Vesna and Vuletić, Mirjana and Ignjatović-Micić, Dragana and Vučinić, Željko",
year = "1999",
abstract = "Plasma membranes were isolated and purified from 14-day-old maize roots (Zea mays L.) by two-phase partitioning at a 6.5% polymer concentration, and compared to isolated mitochondria, microsomes, and soluble fraction. Marker enzyme analysis demonstrated that the plasma membranes were devoid of cytoplasmic, mitochondrial, tonoplast, and endoplasmic-reticulum contaminations. Isolated plasma membranes exhibited malate dehydrogenase activity, catalyzing NADH-dependent reduction of oxaloacetate as well as NAD + -dependent malate oxidation. Malate dehydrogenase activity was resistant to osmotic shock, freeze-thaw treatment, and salt washing and stimulated by solubilization with Triton X-100, indicating that the enzyme is tightly bound to the plasma membrane. Malate dehydrogenase activity was highly specific to NAD + and NADH. The enzyme exhibited a high degree of latency in both right-side-out (80%) and inside-out (70%) vesicle preparations. Kinetic and regulatory properties with ATP and P(i), as well as pH dependence of plasma-membrane-bound malate dehydrogenase were different from mitochondrial and soluble malate dehydrogenases. Starch gel electrophoresis revealed a characteristic isozyme form present in the plasma membrane isolate, but not present in the soluble, mitochondrial, and microsomal fractions. The results presented show that purified plasma membranes isolated from maize roots contain a tightly associated malate dehydrogenase, having properties different from mitochondrial and soluble malate dehydrogenases.",
journal = "Protoplasma",
title = "Plasma-membrane-bound malate dehydrogenase activity in maize roots",
volume = "207",
number = "3-4",
pages = "203-212",
url = "conv_1071"
}
Hadži-Tašković-Šukalović, V., Vuletić, M., Ignjatović-Micić, D.,& Vučinić, Ž.. (1999). Plasma-membrane-bound malate dehydrogenase activity in maize roots. in Protoplasma, 207(3-4), 203-212.
conv_1071
Hadži-Tašković-Šukalović V, Vuletić M, Ignjatović-Micić D, Vučinić Ž. Plasma-membrane-bound malate dehydrogenase activity in maize roots. in Protoplasma. 1999;207(3-4):203-212.
conv_1071 .
Hadži-Tašković-Šukalović, Vesna, Vuletić, Mirjana, Ignjatović-Micić, Dragana, Vučinić, Željko, "Plasma-membrane-bound malate dehydrogenase activity in maize roots" in Protoplasma, 207, no. 3-4 (1999):203-212,
conv_1071 .

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