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Determiinacija KTI u genotipovima soje

dc.creatorKovačević, Dragan
dc.creatorMladenović-Drinić, Snežana
dc.creatorPerić, Vesna
dc.creatorSrebrić, Mirjana
dc.date.accessioned2019-05-16T12:18:11Z
dc.date.available2019-05-16T12:18:11Z
dc.date.issued2015
dc.identifier.issn0354-5881
dc.identifier.urihttp://rik.mrizp.rs/handle/123456789/574
dc.description.abstractKunitz trypsin inhibitor is major antinutritive factor in soybean seed that decrease its nutritional value. Breeding programs aimed to select soybean variety without Kunitz trypsin inhibitor are of great importance as that varieties can be used directly for monogastric animal feeding. By specific markers analysis is possible to detect genotypes without KTI. Ninety sixth soybean genotypes from Soybean collection of Maize Research Institute are analyzed by two markers Satt228 and Satt409. 89 genotypes with both markers had characteristic alleles for genotypes with KTI, and three genotypes Kunitz, Laura and Lana have characteristics bands, with Satt228 alel 2 as with Satt409 band about 170kDa, for genotypes with titi recesive allele. Four genotypes which had both alleles with marker Satt228 with Satt 409 had only allele characteristic for TiTi genotype. Obtained results confirmed that both markers could be used for identification and selection genotypes without Kunitz trypsin inhibitor.en
dc.description.abstractKunitz tripsin inhibitor je glavni antinutritivni faktor u zrnu soje koji smanjuje njenu nutritivnu vrednost. Programi oplemenjivanja koji imaju za cilj stvaranje sorti soje bez ovog proteina su od velikog značaja jer omogućavaju direktnu upotrebu tih sorti za ishranu domaćih životinja. Primenom specifičnih molekularnih markera moguće je identifikovati genotipove koji nemaju Kunitz tripsin inhibitor. Devedeset šest genotipova soje iz kolekcije Instituta za kukuruz su analizirani sa dva markera Satt228 i Satt409. Od analiziranih genotipova 89 genotipova su sa oba markera imali karakteristične allele za genotipove sa KTi, tri genotipa Kunitz, Laura i Lana su pokazali prisustvo karakterističnih traka, sa Satt228 alela2 a sa Satt409 trake veličine oko 170kDa, za genotipove sa titi recesivnim alelom. Četiri genotipa koji su sa markerom Satt228 pokazali prisustvo oba alela su sa Satt 409 imali samo karakterističan alel za TiTi genotip. Dobijeni rezultati su potvrdili da se oba markera mogu koristiti za identifikaciju i selekcionisanje genotipova bez Kunitz tripsin inhibitora.sr
dc.publisherDruštvo selekcionara i semenara Republike Srbije, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31068/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-sa/4.0/
dc.sourceSelekcija i semenarstvo
dc.subjectsoybeanen
dc.subjectKunitz trypsin inhibitoren
dc.subjectmolecular markeren
dc.subjectmolekularni markerisr
dc.subjectKunitz tripsin inhibitorsr
dc.subjectsojasr
dc.titleDetermination of KTI in soybean genotypesen
dc.titleDetermiinacija KTI u genotipovima sojesr
dc.typearticle
dc.rights.licenseBY-SA
dc.citation.volume21
dc.citation.issue1
dc.citation.spage1
dc.citation.epage7
dc.citation.other21(1): 1-7
dc.citation.rankM52
dc.identifier.doi10.5937/SelSem1501001K
dc.identifier.fulltexthttp://rik.mrizp.rs//bitstream/id/2511/572.pdf
dc.identifier.rcubconv_299
dc.type.versionpublishedVersion


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