High-performance thin-layer chromatography (HPTLC) combined with image analysis and pattern recognition methods were used for fingerprinting of phenolic compounds present in seedlings of two maize genotypes ZP 434 (new generation hybrid, drought tolerant) and ZP 704 (older generation hybrid, drought sensitive) treated with different concentrations of 24-epibrassinolide. This is the first report of TLC chromatographic profile of phenolics' mixtures in maize seed extracts influenced by brassinosteroid phytohormones. Nine samples of shoot of seedlings for the whole concentration range of phytohormones (5.2 x 10(-7) -5.2 x 10(-15) M), one sample of root of seedlings treated with 5.2 x 10(-15) M 24-epibrassinolide, and the control samples of nontreated seedlings, for both genotypes, were analyzed. Phenolic profiles of root extracts indicate the absence of more polar compounds such as phenolic acids and glycosides present in shoot of seedlings. Also, hormones applied in higher concentrations... have an inhibiting effect on the content of phenolics in ZP 434. Application of chemometric methods enables characterization of particular genotype of maize according to its phenolic profile.
TY - JOUR
AU - Waisi, Hadi
AU - Kosović, Aleksandra
AU - Krstić, Durda
AU - Milojković-Opsenica, Dušanka
AU - Nikolić, Bogdan
AU - Dragičević, Vesna
AU - Trifković, Jelena
PY - 2015
UR - http://rik.mrizp.rs/handle/123456789/572
AB - High-performance thin-layer chromatography (HPTLC) combined with image analysis and pattern recognition methods were used for fingerprinting of phenolic compounds present in seedlings of two maize genotypes ZP 434 (new generation hybrid, drought tolerant) and ZP 704 (older generation hybrid, drought sensitive) treated with different concentrations of 24-epibrassinolide. This is the first report of TLC chromatographic profile of phenolics' mixtures in maize seed extracts influenced by brassinosteroid phytohormones. Nine samples of shoot of seedlings for the whole concentration range of phytohormones (5.2 x 10(-7) -5.2 x 10(-15) M), one sample of root of seedlings treated with 5.2 x 10(-15) M 24-epibrassinolide, and the control samples of nontreated seedlings, for both genotypes, were analyzed. Phenolic profiles of root extracts indicate the absence of more polar compounds such as phenolic acids and glycosides present in shoot of seedlings. Also, hormones applied in higher concentrations have an inhibiting effect on the content of phenolics in ZP 434. Application of chemometric methods enables characterization of particular genotype of maize according to its phenolic profile.
PB - Hindawi Publishing Corporation, New York
T2 - Journal of Chemistry
T1 - Polyphenolic Profile of Maize Seedlings Treated with 24-Epibrassinolide
DO - 10.1155/2015/976971
ER -
@article{
author = "Waisi, Hadi and Kosović, Aleksandra and Krstić, Durda and Milojković-Opsenica, Dušanka and Nikolić, Bogdan and Dragičević, Vesna and Trifković, Jelena",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/572",
abstract = "High-performance thin-layer chromatography (HPTLC) combined with image analysis and pattern recognition methods were used for fingerprinting of phenolic compounds present in seedlings of two maize genotypes ZP 434 (new generation hybrid, drought tolerant) and ZP 704 (older generation hybrid, drought sensitive) treated with different concentrations of 24-epibrassinolide. This is the first report of TLC chromatographic profile of phenolics' mixtures in maize seed extracts influenced by brassinosteroid phytohormones. Nine samples of shoot of seedlings for the whole concentration range of phytohormones (5.2 x 10(-7) -5.2 x 10(-15) M), one sample of root of seedlings treated with 5.2 x 10(-15) M 24-epibrassinolide, and the control samples of nontreated seedlings, for both genotypes, were analyzed. Phenolic profiles of root extracts indicate the absence of more polar compounds such as phenolic acids and glycosides present in shoot of seedlings. Also, hormones applied in higher concentrations have an inhibiting effect on the content of phenolics in ZP 434. Application of chemometric methods enables characterization of particular genotype of maize according to its phenolic profile.",
publisher = "Hindawi Publishing Corporation, New York",
journal = "Journal of Chemistry",
title = "Polyphenolic Profile of Maize Seedlings Treated with 24-Epibrassinolide",
doi = "10.1155/2015/976971"
}
Waisi, H., Kosović, A., Krstić, D., Milojković-Opsenica, D., Nikolić, B., Dragičević, V.,& Trifković, J. (2015). Polyphenolic Profile of Maize Seedlings Treated with 24-Epibrassinolide.
Journal of Chemistry
Hindawi Publishing Corporation, New York..
https://doi.org/10.1155/2015/976971
Waisi H, Kosović A, Krstić D, Milojković-Opsenica D, Nikolić B, Dragičević V, Trifković J. Polyphenolic Profile of Maize Seedlings Treated with 24-Epibrassinolide. Journal of Chemistry. 2015;
Waisi Hadi, Kosović Aleksandra, Krstić Durda, Milojković-Opsenica Dušanka, Nikolić Bogdan, Dragičević Vesna, Trifković Jelena, "Polyphenolic Profile of Maize Seedlings Treated with 24-Epibrassinolide" Journal of Chemistry (2015),
https://doi.org/10.1155/2015/976971 .
