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Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers

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Authors
Lučić, Aleksandar
Popović, Vladan
Rakonjac, Ljubinko
Ćirković-Mitrović, Tatjana
Nevenić, Marija
Ristić, Danijela
Mladenović-Drinić, Snežana
Article (Published version)
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Abstract
This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mu g/mu l. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i. e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically inte...rpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).

Keywords:
Scots pine / SSR / UPGMA / correspondence analysis
Source:
Archives of Biological Sciences, 2014, 66, 4, 1485-1492
Publisher:
  • Srpsko biološko društvo, Beograd, i dr.
Projects:
  • The development of technological procedures in forestry with a view to an optimum forest cover realisation (RS-31070)

DOI: 10.2298/ABS1404485L

ISSN: 0354-4664

WoS: 000346477900023

Scopus: 2-s2.0-84921646941
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URI
http://rik.mrizp.rs/handle/123456789/537
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