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Poređenje morfoloških i molekularnih genetičkih distanci linija kukuruza

dc.creatorBabić, Milosav
dc.creatorBabić, Vojka
dc.creatorProdanović, Slaven
dc.creatorFilipović, Milomir
dc.creatorAnđelković, Violeta
dc.date.accessioned2019-05-16T12:15:26Z
dc.date.available2019-05-16T12:15:26Z
dc.date.issued2012
dc.identifier.issn0534-0012
dc.identifier.urihttp://rik.mrizp.rs/handle/123456789/465
dc.description.abstractDue to an unknown mechanism of genetic control and great environmental effects in the process of trait expression, morphological markers are often considered unreliable indicators of genetic relationships. Morphological characterization of 19 maize inbreds was done according to the UPOV descriptor, while molecular characterization was performed with RAPD markers. Based on the estimation of phenotypes according to the UPOV descriptor, the squared Euclidean distance was calculated and then, on the basis of this distance, a morphological similarity matrix was formed. Jaccard similarity coefficients were calculated on the basis of presence absence of bands on gels in the RAPD analysis. When data were standardized, the comparison between morphological and genetic similarity of observed maize inbreds was done. The correlations varied from 0.47 (inbred L 217) to 0.76 (inbred L 86). The average value of correlations for all studied inbreds amounted to 0.64. Furthermore, the results of the cluster analysis for both markers, molecular and morphological, had high concordance with pedigree data. Environmental effects were decreased in morphological markers (according to the UPOV descriptor) by rescaling a measurement scale from a scale to an ordinal level of measurement and in such a way results of morphological markers approached results of molecular markers in the estimation of the genetic distance (GD) of maize inbred lines.en
dc.description.abstractZbog nepoznatog mehanizma genetičke kontrole i velikog uticaja spoljne sredine u procesu ekspresije svojstava, o morfološkim markerima se cesto govori kao o nepouzdanim pokazateljima genetičkih odnosa. Morfološka karakterizacija 19 linija kukuruza je urađena po UPOV deskriptoru, vizuelnom ocenom grupe biljka, dok je molekularna karakterizacija urađena RAPD markerima. Na osnovu ocena fenotipa po UPOV deskriptoru izračunati su kvadrati euklidskog rastojanja na osnovu kojih je formirana matrica morfoloških sličnosti. Na osnovu prisustva-odsustva traka na gelovima u RAPD nanalizi izračunati su koeficijenti genetičkih sličnosti po Jaccardu. Nakon toga vršena su poređenja morfoloških i genetičkih sličnosti ispitivanih linija kukuruza. Korelacije su se kretale od 0.47 za liniju L 217 do 0.76 za liniju L 86. Prosečna vrednost korelacija za sve ispitivane linije je iznosila 0.64. Rezultati klaster analize, kako za molekularne tako i za morfološke markere, bili su u visokoj saglasnosti sa pedgre podacima. Degradiranjem merne skale sa skalnog na ordinarni nivo merenja, kod morfoloških markera (po UPOV deskriptoru), je ublažen efekat spoljne sredine. Na taj način su se rezultati morfoloških markera približili rezultatima molekularnih makera u proceni GD inbred linija kukuruza.sr
dc.publisherDruštvo genetičara Srbije, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31068/RS//
dc.rightsopenAccess
dc.sourceGenetika
dc.subjectmaize inbredsen
dc.subjectmolecular and morphological markersen
dc.subjectUPOV descriptoren
dc.titleComparison of morphological and molecular genetic distances of maize inbredsen
dc.titlePoređenje morfoloških i molekularnih genetičkih distanci linija kukuruzasr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.volume44
dc.citation.issue1
dc.citation.spage119
dc.citation.epage128
dc.citation.other44(1): 119-128
dc.citation.rankM23
dc.identifier.wos000306166800012
dc.identifier.doi10.2298/GENSR1201119B
dc.identifier.scopus2-s2.0-84866933740
dc.identifier.fulltexthttp://rik.mrizp.rs//bitstream/id/2399/463.pdf
dc.identifier.rcubconv_462
dc.type.versionpublishedVersion


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