Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds
Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu
Abstract
Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi) concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The investigated extraction mediums: double distilled (DD) H2O, 2.4 % HCl and 5 % trichloracetic acid (TCA) were proved to be suitable for the analysis of phytic acid and inorganic phosphorus in seed extracts. The advantages of 5 % TCA over to DD H2O and 2.4 % HCl were reflected through the low limit of detection for both phytic acid and Pi and good recovery with low bias. A low detection limit for Pi is important for samples with naturally low Pi concentrations, such as soybean seeds.
Fitat je važan za razvoj semena i služi za skladištenje minerala. On se uglavnom smatra za antinutritivnu supstancu. Cilj istraživanja je bio da se razvije brza i jeftina kolorimetrijska metoda za merenje koncentracije fitata i neorganskog fosfora (Pi) iz ekstrakta semena kukuruza, soje i suncokreta, kombinujući određenu preciznost i jednostavnost, idealne za selekcionere zainte- resovane za istovremeno poboljšanje odnosa Pi i fitata u semenu. Ispitivana ekstrakciona sredstva: bidestilovana voda (DD H2O), 2,4 % HCl i 5 % trihlorsirćetna kiselina (TCA) su se pokazale pogodnim za analizu fitinske kiseline i Pi iz ekstrakta semena. Prednost 5 % TCA u odnosu na DD H2O i 2,4 % HCl se ogleda u niskoj granici detekcije za fitinsku kiselinu i Pi, dobrom recoveryju i niskim vrednostima odstupanja (bias). Niska granica detekcije za Pi je važna za uzorke sa prirodno niskom Pi koncentracijom, kao što je seme soje.
Keywords:
phytic acid / inorganic phosphorus / seed / validation / fitinska kiselina / neorganski fosfor / seme / validacijaSource:
Acta periodica technologica, 2011, 42, 11-21Publisher:
- Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad
Funding / projects:
Collections
Institution/Community
MRIZPTY - JOUR AU - Dragičević, Vesna AU - Sredojević, Slobodanka AU - Perić, Vesna AU - Kovinčić, Anika AU - Srebrić, Mirjana PY - 2011 UR - http://rik.mrizp.rs/handle/123456789/405 AB - Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi) concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The investigated extraction mediums: double distilled (DD) H2O, 2.4 % HCl and 5 % trichloracetic acid (TCA) were proved to be suitable for the analysis of phytic acid and inorganic phosphorus in seed extracts. The advantages of 5 % TCA over to DD H2O and 2.4 % HCl were reflected through the low limit of detection for both phytic acid and Pi and good recovery with low bias. A low detection limit for Pi is important for samples with naturally low Pi concentrations, such as soybean seeds. AB - Fitat je važan za razvoj semena i služi za skladištenje minerala. On se uglavnom smatra za antinutritivnu supstancu. Cilj istraživanja je bio da se razvije brza i jeftina kolorimetrijska metoda za merenje koncentracije fitata i neorganskog fosfora (Pi) iz ekstrakta semena kukuruza, soje i suncokreta, kombinujući određenu preciznost i jednostavnost, idealne za selekcionere zainte- resovane za istovremeno poboljšanje odnosa Pi i fitata u semenu. Ispitivana ekstrakciona sredstva: bidestilovana voda (DD H2O), 2,4 % HCl i 5 % trihlorsirćetna kiselina (TCA) su se pokazale pogodnim za analizu fitinske kiseline i Pi iz ekstrakta semena. Prednost 5 % TCA u odnosu na DD H2O i 2,4 % HCl se ogleda u niskoj granici detekcije za fitinsku kiselinu i Pi, dobrom recoveryju i niskim vrednostima odstupanja (bias). Niska granica detekcije za Pi je važna za uzorke sa prirodno niskom Pi koncentracijom, kao što je seme soje. PB - Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad T2 - Acta periodica technologica T1 - Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds T1 - Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu IS - 42 SP - 11 EP - 21 DO - 10.2298/APT1142011D UR - conv_552 ER -
@article{ author = "Dragičević, Vesna and Sredojević, Slobodanka and Perić, Vesna and Kovinčić, Anika and Srebrić, Mirjana", year = "2011", abstract = "Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi) concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The investigated extraction mediums: double distilled (DD) H2O, 2.4 % HCl and 5 % trichloracetic acid (TCA) were proved to be suitable for the analysis of phytic acid and inorganic phosphorus in seed extracts. The advantages of 5 % TCA over to DD H2O and 2.4 % HCl were reflected through the low limit of detection for both phytic acid and Pi and good recovery with low bias. A low detection limit for Pi is important for samples with naturally low Pi concentrations, such as soybean seeds., Fitat je važan za razvoj semena i služi za skladištenje minerala. On se uglavnom smatra za antinutritivnu supstancu. Cilj istraživanja je bio da se razvije brza i jeftina kolorimetrijska metoda za merenje koncentracije fitata i neorganskog fosfora (Pi) iz ekstrakta semena kukuruza, soje i suncokreta, kombinujući određenu preciznost i jednostavnost, idealne za selekcionere zainte- resovane za istovremeno poboljšanje odnosa Pi i fitata u semenu. Ispitivana ekstrakciona sredstva: bidestilovana voda (DD H2O), 2,4 % HCl i 5 % trihlorsirćetna kiselina (TCA) su se pokazale pogodnim za analizu fitinske kiseline i Pi iz ekstrakta semena. Prednost 5 % TCA u odnosu na DD H2O i 2,4 % HCl se ogleda u niskoj granici detekcije za fitinsku kiselinu i Pi, dobrom recoveryju i niskim vrednostima odstupanja (bias). Niska granica detekcije za Pi je važna za uzorke sa prirodno niskom Pi koncentracijom, kao što je seme soje.", publisher = "Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad", journal = "Acta periodica technologica", title = "Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds, Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu", number = "42", pages = "11-21", doi = "10.2298/APT1142011D", url = "conv_552" }
Dragičević, V., Sredojević, S., Perić, V., Kovinčić, A.,& Srebrić, M.. (2011). Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds. in Acta periodica technologica Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad.(42), 11-21. https://doi.org/10.2298/APT1142011D conv_552
Dragičević V, Sredojević S, Perić V, Kovinčić A, Srebrić M. Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds. in Acta periodica technologica. 2011;(42):11-21. doi:10.2298/APT1142011D conv_552 .
Dragičević, Vesna, Sredojević, Slobodanka, Perić, Vesna, Kovinčić, Anika, Srebrić, Mirjana, "Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds" in Acta periodica technologica, no. 42 (2011):11-21, https://doi.org/10.2298/APT1142011D ., conv_552 .