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Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds

Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu

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2011
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Authors
Dragičević, Vesna
Sredojević, Slobodanka
Perić, Vesna
Kovinčić, Anika
Srebrić, Mirjana
Article (Published version)
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Abstract
Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi) concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The investigated extraction mediums: double distilled (DD) H2O, 2.4 % HCl and 5 % trichloracetic acid (TCA) were proved to be suitable for the analysis of phytic acid and inorganic phosphorus in seed extracts. The advantages of 5 % TCA over to DD H2O and 2.4 % HCl were reflected through the low limit of detection for both phytic acid and Pi and good recovery with low bias. A low detection limit for Pi is important for samples with naturally low Pi concentrations, such as soybean seeds.
Fitat je važan za razvoj semena i služi za skladištenje minerala. On se uglavnom smatra za antinutritivnu supstancu. Cilj istraživanja je bio da se razvije brza i jeftina kolorimetrijska metoda za merenje koncentracije fitata i neorganskog fosfora (Pi) iz ekstrakta semena kukuruza, soje i suncokreta, kombinujući određenu preciznost i jednostavnost, idealne za selekcionere zainte- resovane za istovremeno poboljšanje odnosa Pi i fitata u semenu. Ispitivana ekstrakciona sredstva: bidestilovana voda (DD H2O), 2,4 % HCl i 5 % trihlorsirćetna kiselina (TCA) su se pokazale pogodnim za analizu fitinske kiseline i Pi iz ekstrakta semena. Prednost 5 % TCA u odnosu na DD H2O i 2,4 % HCl se ogleda u niskoj granici detekcije za fitinsku kiselinu i Pi, dobrom recoveryju i niskim vrednostima odstupanja (bias). Niska granica detekcije za Pi je važna za uzorke sa prirodno niskom Pi koncentracijom, kao što je seme soje.
Keywords:
phytic acid / inorganic phosphorus / seed / validation / fitinska kiselina / neorganski fosfor / seme / validacija
Source:
Acta periodica technologica, 2011, 42, 11-21
Publisher:
  • Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad
Funding / projects:
  • Integrated field crop production: conservation of biodiversity and soil fertility (RS-31037)

DOI: 10.2298/APT1142011D

ISSN: 1450-7188

Scopus: 2-s2.0-84858307788
[ Google Scholar ]
29
URI
http://rik.mrizp.rs/handle/123456789/405
Collections
  • Радови истраживача / Researchers' publications
Institution/Community
MRIZP
TY  - JOUR
AU  - Dragičević, Vesna
AU  - Sredojević, Slobodanka
AU  - Perić, Vesna
AU  - Kovinčić, Anika
AU  - Srebrić, Mirjana
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/405
AB  - Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi) concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The investigated extraction mediums: double distilled (DD) H2O, 2.4 % HCl and 5 % trichloracetic acid (TCA) were proved to be suitable for the analysis of phytic acid and inorganic phosphorus in seed extracts. The advantages of 5 % TCA over to DD H2O and 2.4 % HCl were reflected through the low limit of detection for both phytic acid and Pi and good recovery with low bias. A low detection limit for Pi is important for samples with naturally low Pi concentrations, such as soybean seeds.
AB  - Fitat je važan za razvoj semena i služi za skladištenje minerala. On se uglavnom smatra za antinutritivnu supstancu. Cilj istraživanja je bio da se razvije brza i jeftina kolorimetrijska metoda za merenje koncentracije fitata i neorganskog fosfora (Pi) iz ekstrakta semena kukuruza, soje i suncokreta, kombinujući određenu preciznost i jednostavnost, idealne za selekcionere zainte- resovane za istovremeno poboljšanje odnosa Pi i fitata u semenu. Ispitivana ekstrakciona sredstva: bidestilovana voda (DD H2O), 2,4 % HCl i 5 % trihlorsirćetna kiselina (TCA) su se pokazale pogodnim za analizu fitinske kiseline i Pi iz ekstrakta semena. Prednost 5 % TCA u odnosu na DD H2O i 2,4 % HCl se ogleda u niskoj granici detekcije za fitinsku kiselinu i Pi, dobrom recoveryju i niskim vrednostima odstupanja (bias). Niska granica detekcije za Pi je važna za uzorke sa prirodno niskom Pi koncentracijom, kao što je seme soje.
PB  - Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad
T2  - Acta periodica technologica
T1  - Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds
T1  - Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu
IS  - 42
SP  - 11
EP  - 21
DO  - 10.2298/APT1142011D
UR  - conv_552
ER  - 
@article{
author = "Dragičević, Vesna and Sredojević, Slobodanka and Perić, Vesna and Kovinčić, Anika and Srebrić, Mirjana",
year = "2011",
abstract = "Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi) concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The investigated extraction mediums: double distilled (DD) H2O, 2.4 % HCl and 5 % trichloracetic acid (TCA) were proved to be suitable for the analysis of phytic acid and inorganic phosphorus in seed extracts. The advantages of 5 % TCA over to DD H2O and 2.4 % HCl were reflected through the low limit of detection for both phytic acid and Pi and good recovery with low bias. A low detection limit for Pi is important for samples with naturally low Pi concentrations, such as soybean seeds., Fitat je važan za razvoj semena i služi za skladištenje minerala. On se uglavnom smatra za antinutritivnu supstancu. Cilj istraživanja je bio da se razvije brza i jeftina kolorimetrijska metoda za merenje koncentracije fitata i neorganskog fosfora (Pi) iz ekstrakta semena kukuruza, soje i suncokreta, kombinujući određenu preciznost i jednostavnost, idealne za selekcionere zainte- resovane za istovremeno poboljšanje odnosa Pi i fitata u semenu. Ispitivana ekstrakciona sredstva: bidestilovana voda (DD H2O), 2,4 % HCl i 5 % trihlorsirćetna kiselina (TCA) su se pokazale pogodnim za analizu fitinske kiseline i Pi iz ekstrakta semena. Prednost 5 % TCA u odnosu na DD H2O i 2,4 % HCl se ogleda u niskoj granici detekcije za fitinsku kiselinu i Pi, dobrom recoveryju i niskim vrednostima odstupanja (bias). Niska granica detekcije za Pi je važna za uzorke sa prirodno niskom Pi koncentracijom, kao što je seme soje.",
publisher = "Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad",
journal = "Acta periodica technologica",
title = "Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds, Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu",
number = "42",
pages = "11-21",
doi = "10.2298/APT1142011D",
url = "conv_552"
}
Dragičević, V., Sredojević, S., Perić, V., Kovinčić, A.,& Srebrić, M.. (2011). Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds. in Acta periodica technologica
Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad.(42), 11-21.
https://doi.org/10.2298/APT1142011D
conv_552
Dragičević V, Sredojević S, Perić V, Kovinčić A, Srebrić M. Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds. in Acta periodica technologica. 2011;(42):11-21.
doi:10.2298/APT1142011D
conv_552 .
Dragičević, Vesna, Sredojević, Slobodanka, Perić, Vesna, Kovinčić, Anika, Srebrić, Mirjana, "Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds" in Acta periodica technologica, no. 42 (2011):11-21,
https://doi.org/10.2298/APT1142011D .,
conv_552 .

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