The structure of sterile cytoplasm types within a maize genebank collection
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2010
Authors
Vančetović, Jelena
Vidaković, M.
Ignjatović-Micić, Dragana

Nikolić, Ana

Marković, Ksenija

Anđelković, Violeta

Article (Published version)

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Maize Research Institute (MRI) gene bank maintains a collection of 6000 maize accessions. Within this collection over 100 sources of cytoplasmic male sterility (CMS) were found in field trials, i.e. more than 2% of the total accession numbers. These sources are distributed among Yugoslav open-pollinated varieties (4.56% of them contain CMS), as well as introduced heterozygous genotypes and inbred lines. In order to identify cytoplasm types the gene-bank sources of CMS were screened using a PCR assay with specific primers for C, T and S cytoplasms. Predominant cytoplasmic male sterility type among the analyzed accessions was CMS-S. Results were inconclusive for three accessions, i.e. different results for the progenies of two ears per accession were obtained. For another two accessions a new PCR product profile was identified, consisting of one band characteristic for CMS-S and one unspecific for any of the three CMS types. The PCR approach enabled a simple, fast and reliable large scal...e screening of maize cytoplasm among MRI gene bank accessions, significantly reducing time for cytoplasm characterizations compared to classical method of testing with restorers for each known type of CMS.
Source:
Russian Journal of Genetics, 2010, 46, 7, 836-840Publisher:
- Maik Nauka/Interperiodica/Springer, New York
DOI: 10.1134/S1022795410070082
ISSN: 1022-7954
WoS: 000280244900008
Scopus: 2-s2.0-77955042055
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MRIZPTY - JOUR AU - Vančetović, Jelena AU - Vidaković, M. AU - Ignjatović-Micić, Dragana AU - Nikolić, Ana AU - Marković, Ksenija AU - Anđelković, Violeta PY - 2010 UR - http://rik.mrizp.rs/handle/123456789/336 AB - Maize Research Institute (MRI) gene bank maintains a collection of 6000 maize accessions. Within this collection over 100 sources of cytoplasmic male sterility (CMS) were found in field trials, i.e. more than 2% of the total accession numbers. These sources are distributed among Yugoslav open-pollinated varieties (4.56% of them contain CMS), as well as introduced heterozygous genotypes and inbred lines. In order to identify cytoplasm types the gene-bank sources of CMS were screened using a PCR assay with specific primers for C, T and S cytoplasms. Predominant cytoplasmic male sterility type among the analyzed accessions was CMS-S. Results were inconclusive for three accessions, i.e. different results for the progenies of two ears per accession were obtained. For another two accessions a new PCR product profile was identified, consisting of one band characteristic for CMS-S and one unspecific for any of the three CMS types. The PCR approach enabled a simple, fast and reliable large scale screening of maize cytoplasm among MRI gene bank accessions, significantly reducing time for cytoplasm characterizations compared to classical method of testing with restorers for each known type of CMS. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Russian Journal of Genetics T1 - The structure of sterile cytoplasm types within a maize genebank collection VL - 46 IS - 7 SP - 836 EP - 840 DO - 10.1134/S1022795410070082 UR - conv_765 ER -
@article{ author = "Vančetović, Jelena and Vidaković, M. and Ignjatović-Micić, Dragana and Nikolić, Ana and Marković, Ksenija and Anđelković, Violeta", year = "2010", abstract = "Maize Research Institute (MRI) gene bank maintains a collection of 6000 maize accessions. Within this collection over 100 sources of cytoplasmic male sterility (CMS) were found in field trials, i.e. more than 2% of the total accession numbers. These sources are distributed among Yugoslav open-pollinated varieties (4.56% of them contain CMS), as well as introduced heterozygous genotypes and inbred lines. In order to identify cytoplasm types the gene-bank sources of CMS were screened using a PCR assay with specific primers for C, T and S cytoplasms. Predominant cytoplasmic male sterility type among the analyzed accessions was CMS-S. Results were inconclusive for three accessions, i.e. different results for the progenies of two ears per accession were obtained. For another two accessions a new PCR product profile was identified, consisting of one band characteristic for CMS-S and one unspecific for any of the three CMS types. The PCR approach enabled a simple, fast and reliable large scale screening of maize cytoplasm among MRI gene bank accessions, significantly reducing time for cytoplasm characterizations compared to classical method of testing with restorers for each known type of CMS.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Russian Journal of Genetics", title = "The structure of sterile cytoplasm types within a maize genebank collection", volume = "46", number = "7", pages = "836-840", doi = "10.1134/S1022795410070082", url = "conv_765" }
Vančetović, J., Vidaković, M., Ignjatović-Micić, D., Nikolić, A., Marković, K.,& Anđelković, V.. (2010). The structure of sterile cytoplasm types within a maize genebank collection. in Russian Journal of Genetics Maik Nauka/Interperiodica/Springer, New York., 46(7), 836-840. https://doi.org/10.1134/S1022795410070082 conv_765
Vančetović J, Vidaković M, Ignjatović-Micić D, Nikolić A, Marković K, Anđelković V. The structure of sterile cytoplasm types within a maize genebank collection. in Russian Journal of Genetics. 2010;46(7):836-840. doi:10.1134/S1022795410070082 conv_765 .
Vančetović, Jelena, Vidaković, M., Ignjatović-Micić, Dragana, Nikolić, Ana, Marković, Ksenija, Anđelković, Violeta, "The structure of sterile cytoplasm types within a maize genebank collection" in Russian Journal of Genetics, 46, no. 7 (2010):836-840, https://doi.org/10.1134/S1022795410070082 ., conv_765 .