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dc.creatorŽilić, Slađana
dc.creatorBarać, Miroljub
dc.creatorPešić, Mirjana
dc.creatorCrevar, Miloš
dc.creatorStanojević, Slađana
dc.creatorKovinčić, Anika
dc.creatorSaratlić, Goran
dc.creatorTolimir, Miodrag
dc.date.accessioned2019-05-16T12:10:55Z
dc.date.available2019-05-16T12:10:55Z
dc.date.issued2010
dc.identifier.issn1018-1806
dc.identifier.urihttp://rik.mrizp.rs/handle/123456789/292
dc.description.abstractTotal sunflower proteins, storage proteins, and helianthinin (11S) and 2S albumin fractions and their respective subunits in seeds and kernels of three sunflower hybrids were analyzed. Protein contents were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and coupled with densitometry. The SDS-PAGE profiles of the seed and kernel proteins in the crude extracts for all genotypes showed a very similar number of protein bands (thirty two) in the electrophoretograms. Three polypeptide groups of helianthinin fraction were detected. Two of these were acidic (α, Mw = 36,800 - 42,900 Da and α', Mw = 31,000 - 35,300 Da), while one was basic (β, Mw=21,000 - 29,600 Da). The molecular weight of the 2S albumin proteins ranged from 11,500 to 20,100 Da. According to our results, there were significant differences among the seed and kernel protein contents. The 2S albumin content was significantly higher in kernels than in whole seeds of sunflower hybrids (P lt 0.05). By contrast, the 11S helianthinin content was significantly higher in seeds (where it ranged from 61.75 to 67.70% of totally extracted proteins) than in kernels (varied from 57.36 to 61.51% of totally extracted proteins) of sunflower hybrids (P lt 0.05).en
dc.publisherInstitut za ratarstvo i povrtarstvo, Novi Sad
dc.rightsopenAccess
dc.sourceHelia
dc.subjectsunfloweren
dc.subjectsoluble protein fractions and subunitsen
dc.titleCharacterization of sunflower seed and kernel proteinsen
dc.typearticle
dc.rights.licenseARR
dc.citation.volume33
dc.citation.issue52
dc.citation.spage103
dc.citation.epage113
dc.citation.other33(52): 103-113
dc.citation.rankM24
dc.identifier.doi10.2298/HEL1052103Z
dc.identifier.scopus2-s2.0-78049291885
dc.identifier.fulltexthttp://rik.mrizp.rs//bitstream/id/2223/290.pdf
dc.identifier.rcubconv_511
dc.type.versionpublishedVersion


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