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dc.creatorIgnjatović-Micić, Dragana
dc.creatorMladenović Drinić, Snežana
dc.creatorNikolić, Ana
dc.creatorLazić-Jančić, Vesna
dc.date.accessioned2019-05-16T12:09:41Z
dc.date.available2019-05-16T12:09:41Z
dc.date.issued2008
dc.identifier.issn0016-6758
dc.identifier.urihttp://rik.mrizp.rs/handle/123456789/245
dc.description.abstractA collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively and G(ST) value was 0.360. Genetic distance values calculated using Modified Rogers' Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.en
dc.publisherDruštvo genetičara Srbije, Beograd
dc.rightsrestrictedAccess
dc.sourceGenetika
dc.titleSsr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.en
dc.typearticle
dc.rights.licenseARR
dc.citation.volume44
dc.citation.issue11
dc.citation.spage1517
dc.citation.epage1524
dc.citation.other44(11): 1517-1524
dc.identifier.scopus2-s2.0-59149083655
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_rik_245
dc.type.versionpublishedVersion


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Приказ основних података о документу