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SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories

Authorized Users Only
2008
Authors
Ignjatović-Micić, Dragana
Mladenović-Drinić, Snežana
Nikolić, Ana
Lazić-Jančić, Vesna
Article (Published version)
Metadata
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Abstract
A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work, 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively, and G(ST) value was 0.360. Genetic distance values calculated using Mod...ified Rogers Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.

Source:
Russian Journal of Genetics, 2008, 44, 11, 1317-1324
Publisher:
  • Maik Nauka/Interperiodica/Springer, New York

DOI: 10.1134/S1022795408110100

ISSN: 1022-7954

WoS: 000263220900010

Scopus: 2-s2.0-60349095765
[ Google Scholar ]
7
10
URI
http://rik.mrizp.rs/handle/123456789/237
Collections
  • Радови истраживача / Researchers' publications
Institution/Community
MRIZP
TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Mladenović-Drinić, Snežana
AU  - Nikolić, Ana
AU  - Lazić-Jančić, Vesna
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/237
AB  - A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work, 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively, and G(ST) value was 0.360. Genetic distance values calculated using Modified Rogers Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Russian Journal of Genetics
T1  - SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories
VL  - 44
IS  - 11
SP  - 1317
EP  - 1324
DO  - 10.1134/S1022795408110100
UR  - conv_718
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Mladenović-Drinić, Snežana and Nikolić, Ana and Lazić-Jančić, Vesna",
year = "2008",
abstract = "A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work, 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively, and G(ST) value was 0.360. Genetic distance values calculated using Modified Rogers Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Russian Journal of Genetics",
title = "SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories",
volume = "44",
number = "11",
pages = "1317-1324",
doi = "10.1134/S1022795408110100",
url = "conv_718"
}
Ignjatović-Micić, D., Mladenović-Drinić, S., Nikolić, A.,& Lazić-Jančić, V.. (2008). SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories. in Russian Journal of Genetics
Maik Nauka/Interperiodica/Springer, New York., 44(11), 1317-1324.
https://doi.org/10.1134/S1022795408110100
conv_718
Ignjatović-Micić D, Mladenović-Drinić S, Nikolić A, Lazić-Jančić V. SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories. in Russian Journal of Genetics. 2008;44(11):1317-1324.
doi:10.1134/S1022795408110100
conv_718 .
Ignjatović-Micić, Dragana, Mladenović-Drinić, Snežana, Nikolić, Ana, Lazić-Jančić, Vesna, "SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories" in Russian Journal of Genetics, 44, no. 11 (2008):1317-1324,
https://doi.org/10.1134/S1022795408110100 .,
conv_718 .

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