Effects of sister-line cross on grain yield increase per soybean line plant with plant with various trypsin inhibitor activity in grain

Abstract

In order to develop the initial population the adapted variety Kador and the variety Kunitz were crossed within the soybean breeding program for reduced trypsin inhibitor activity. The variety Kunitz is characterized by the absence of Kunitz trypsin inhibitor in grain. After testing of progenies of the most yielding F2 plants three lines, designated as L6, L30 and L38, were selected for a further procedure. The line L38 is identified as a genotype homozygous for the absence of Kunitz trypsin inhibitor. Data obtained from crosses of two sister combinations. L30 X L38 and L6 X L30, were used in this study. Grain yield of progenies of both, lines L30 and L6 and crosses of both combinations, was evaluated. Approximately a half of cross progenies over yielded progenies of, lines L30 and L6 regardless of the combination of crosses and trypsin inhibitor activity in grain. Superior progenies of both combinations of sister crosses were selected form further studies. The combination L30 x L38 was the most superior in relation to grain yield per plant and frequency of genotypes with reduced trypsin inhibitor activity in grain.

Iz početne populacije formirane ukrštanjem Kunitz x Kador (sorta Kunitz se odlikuje odsustvom Kunitz tripsin inhibitora u zrnu) posle testiranja potomstva najrodnijih F2 biljaka odabrane su tri linije za dalji postupak označene kao L6. L30 i L38. Linija L38 je identifikovana kao genotip homozigotan za odsustvo Kunitz tripsin inhibitora. U ovom radu su korišćeni podaci dobijeni iz dve sestrinske kombinacije ukrštanju i to L30X L3S i L6 X L30. Obavljeno je testiranje prinosa zrna potomstva majčinskih biljaka i potomstva ukrštanja iz obe kombinacije. Približno polovina potomstva ukrštanja je postigla veće prinose zrna po biljci u odnosu na potomstva majki, bez obzira na kombinaciju ukrštanja i tripsin inhibitorsku aktivnost u zrnu. Kombinacija L30xL38je superiornija i po prinosu zrna po biljci i po frekvenciji genotipova sa smanjenom aktivnošću tripsin inhibitora u zrnu.