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Comparison of AFLP and SSR markers for genetic diversity studies in maize populations

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Authors
Ignjatović-Micić, Dragana
Mladenović-Drinić, Snežana
Nikolić, Ana
Lazić-Jančić, Vesna
Article (Published version)
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Abstract
Using a set of 18 maize populations belonging to six different agro-ecological groups we report on a comparison of AFLP and SSR techniques to evaluate their applicability for the study of genetic diversity. Also, a DNA-pooling strategy (bulked samples) that proved to be effective in RFLP analysis of maize populations was employed in order to certify if it could be applied with the same success with SSR and AFLP markers. Twenty-five SSR markers generated a total of 224 polymorphic bands, while three AFLP primer combinations generated 188 bands with 79.2% being polymorphic. The average polymorphism content (PIC) was higher for SSRs, i.e. 0.79 for SSRs versus 0.28 for AFLPs. Higher genetic similarity (GS) values were estimated for AFLPs. The Pearson's correlation coefficient calculated for SSR and AFLP genetic similarities was low (0.149) indicating a poor agreement between the two marker systems. The ranking of the agroecological groups according to their mean GS values (the average GS c...alculated for the three populations representing each group) was congruent for both marker systems and the Pearson's correlation coefficient calculated using these values was 0.853. The cluster analysis did not group populations precisely with expectations based upon their agro-ecological groups, although a much better agreement was accomplished with AFLP analysis. Based on the results in this work it could be concluded that, when using a DNA-pooling strategy, AFLPs are more reliable in assessing genetic diversity of maize populations.

Keywords:
AFLP / bulks / genetic relationships / maize populations / SSR
Source:
Maydica, 2007, 52, 4, 399-406
Publisher:
  • Maydica-Ist Sper Cerealicoltur, Bergamo

ISSN: 0025-6153

WoS: 000253740800003

Scopus: 2-s2.0-40449134782
[ Google Scholar ]
6
7
URI
http://rik.mrizp.rs/handle/123456789/175
Collections
  • Радови истраживача / Researchers' publications
Institution/Community
MRIZP
TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Mladenović-Drinić, Snežana
AU  - Nikolić, Ana
AU  - Lazić-Jančić, Vesna
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/175
AB  - Using a set of 18 maize populations belonging to six different agro-ecological groups we report on a comparison of AFLP and SSR techniques to evaluate their applicability for the study of genetic diversity. Also, a DNA-pooling strategy (bulked samples) that proved to be effective in RFLP analysis of maize populations was employed in order to certify if it could be applied with the same success with SSR and AFLP markers. Twenty-five SSR markers generated a total of 224 polymorphic bands, while three AFLP primer combinations generated 188 bands with 79.2% being polymorphic. The average polymorphism content (PIC) was higher for SSRs, i.e. 0.79 for SSRs versus 0.28 for AFLPs. Higher genetic similarity (GS) values were estimated for AFLPs. The Pearson's correlation coefficient calculated for SSR and AFLP genetic similarities was low (0.149) indicating a poor agreement between the two marker systems. The ranking of the agroecological groups according to their mean GS values (the average GS calculated for the three populations representing each group) was congruent for both marker systems and the Pearson's correlation coefficient calculated using these values was 0.853. The cluster analysis did not group populations precisely with expectations based upon their agro-ecological groups, although a much better agreement was accomplished with AFLP analysis. Based on the results in this work it could be concluded that, when using a DNA-pooling strategy, AFLPs are more reliable in assessing genetic diversity of maize populations.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - Comparison of AFLP and SSR markers for genetic diversity studies in maize populations
VL  - 52
IS  - 4
SP  - 399
EP  - 406
UR  - conv_701
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Mladenović-Drinić, Snežana and Nikolić, Ana and Lazić-Jančić, Vesna",
year = "2007",
abstract = "Using a set of 18 maize populations belonging to six different agro-ecological groups we report on a comparison of AFLP and SSR techniques to evaluate their applicability for the study of genetic diversity. Also, a DNA-pooling strategy (bulked samples) that proved to be effective in RFLP analysis of maize populations was employed in order to certify if it could be applied with the same success with SSR and AFLP markers. Twenty-five SSR markers generated a total of 224 polymorphic bands, while three AFLP primer combinations generated 188 bands with 79.2% being polymorphic. The average polymorphism content (PIC) was higher for SSRs, i.e. 0.79 for SSRs versus 0.28 for AFLPs. Higher genetic similarity (GS) values were estimated for AFLPs. The Pearson's correlation coefficient calculated for SSR and AFLP genetic similarities was low (0.149) indicating a poor agreement between the two marker systems. The ranking of the agroecological groups according to their mean GS values (the average GS calculated for the three populations representing each group) was congruent for both marker systems and the Pearson's correlation coefficient calculated using these values was 0.853. The cluster analysis did not group populations precisely with expectations based upon their agro-ecological groups, although a much better agreement was accomplished with AFLP analysis. Based on the results in this work it could be concluded that, when using a DNA-pooling strategy, AFLPs are more reliable in assessing genetic diversity of maize populations.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "Comparison of AFLP and SSR markers for genetic diversity studies in maize populations",
volume = "52",
number = "4",
pages = "399-406",
url = "conv_701"
}
Ignjatović-Micić, D., Mladenović-Drinić, S., Nikolić, A.,& Lazić-Jančić, V.. (2007). Comparison of AFLP and SSR markers for genetic diversity studies in maize populations. in Maydica
Maydica-Ist Sper Cerealicoltur, Bergamo., 52(4), 399-406.
conv_701
Ignjatović-Micić D, Mladenović-Drinić S, Nikolić A, Lazić-Jančić V. Comparison of AFLP and SSR markers for genetic diversity studies in maize populations. in Maydica. 2007;52(4):399-406.
conv_701 .
Ignjatović-Micić, Dragana, Mladenović-Drinić, Snežana, Nikolić, Ana, Lazić-Jančić, Vesna, "Comparison of AFLP and SSR markers for genetic diversity studies in maize populations" in Maydica, 52, no. 4 (2007):399-406,
conv_701 .

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