Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers
Identifikacija tipova citoplazmatične sterilnosti kukuruza primenom specifičnih prajmera mtDNK

2006
Authors
Ignjatović-Micić, Dragana
Nikolić, Ana

Mladenović-Drinić, Snežana

Vančetović, Jelena

Lazić-Jančić, Vesna
Article (Published version)
Metadata
Show full item recordAbstract
Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank "Zemun Polje", distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of CMS (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of CMS.
Trideset izvora citoplazmatične muške sterilnosti (CMS) u okviru lokalnih populacija iz Banke gena Instituta za kukuruz "Zemun Polje" je testirano na prisustvo odgovarajućeg tipa citoplazme multipleks PCR metodom, korišćenjem specifičnih prajmera za T, C i S citoplazmu. Kombinovanje tri para prajmera u jednoj PCR reakciji, koji odgovaraju himernim regionima mitohondrijalnih DNK sekvenci specifičnih za svaki tip citoplazme, omogućilo je pouzdanu identifikaciju glavnih tipova sterilne citoplazme. Detektovano je dominantno prisustvo S tipa citoplazme. Za izvore sterilnosti kod kojih nije identifikovan tip sterilne citoplazme (odsustvo PCR trake) postoji realna sumnja da se radi o novim, neidentifikovanim tipovima citoplazmatične muške sterilnosti.
Keywords:
CMS / maize / multiplex PCRSource:
Genetika, 2006, 38, 3, 227-233Publisher:
- Društvo genetičara Srbije, Beograd
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MRIZPTY - JOUR AU - Ignjatović-Micić, Dragana AU - Nikolić, Ana AU - Mladenović-Drinić, Snežana AU - Vančetović, Jelena AU - Lazić-Jančić, Vesna PY - 2006 UR - http://rik.mrizp.rs/handle/123456789/139 AB - Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank "Zemun Polje", distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of CMS (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of CMS. AB - Trideset izvora citoplazmatične muške sterilnosti (CMS) u okviru lokalnih populacija iz Banke gena Instituta za kukuruz "Zemun Polje" je testirano na prisustvo odgovarajućeg tipa citoplazme multipleks PCR metodom, korišćenjem specifičnih prajmera za T, C i S citoplazmu. Kombinovanje tri para prajmera u jednoj PCR reakciji, koji odgovaraju himernim regionima mitohondrijalnih DNK sekvenci specifičnih za svaki tip citoplazme, omogućilo je pouzdanu identifikaciju glavnih tipova sterilne citoplazme. Detektovano je dominantno prisustvo S tipa citoplazme. Za izvore sterilnosti kod kojih nije identifikovan tip sterilne citoplazme (odsustvo PCR trake) postoji realna sumnja da se radi o novim, neidentifikovanim tipovima citoplazmatične muške sterilnosti. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers T1 - Identifikacija tipova citoplazmatične sterilnosti kukuruza primenom specifičnih prajmera mtDNK VL - 38 IS - 3 SP - 227 EP - 233 DO - 10.2298/GENSR0603227I UR - conv_401 ER -
@article{ author = "Ignjatović-Micić, Dragana and Nikolić, Ana and Mladenović-Drinić, Snežana and Vančetović, Jelena and Lazić-Jančić, Vesna", year = "2006", abstract = "Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank "Zemun Polje", distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of CMS (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of CMS., Trideset izvora citoplazmatične muške sterilnosti (CMS) u okviru lokalnih populacija iz Banke gena Instituta za kukuruz "Zemun Polje" je testirano na prisustvo odgovarajućeg tipa citoplazme multipleks PCR metodom, korišćenjem specifičnih prajmera za T, C i S citoplazmu. Kombinovanje tri para prajmera u jednoj PCR reakciji, koji odgovaraju himernim regionima mitohondrijalnih DNK sekvenci specifičnih za svaki tip citoplazme, omogućilo je pouzdanu identifikaciju glavnih tipova sterilne citoplazme. Detektovano je dominantno prisustvo S tipa citoplazme. Za izvore sterilnosti kod kojih nije identifikovan tip sterilne citoplazme (odsustvo PCR trake) postoji realna sumnja da se radi o novim, neidentifikovanim tipovima citoplazmatične muške sterilnosti.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers, Identifikacija tipova citoplazmatične sterilnosti kukuruza primenom specifičnih prajmera mtDNK", volume = "38", number = "3", pages = "227-233", doi = "10.2298/GENSR0603227I", url = "conv_401" }
Ignjatović-Micić, D., Nikolić, A., Mladenović-Drinić, S., Vančetović, J.,& Lazić-Jančić, V.. (2006). Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers. in Genetika Društvo genetičara Srbije, Beograd., 38(3), 227-233. https://doi.org/10.2298/GENSR0603227I conv_401
Ignjatović-Micić D, Nikolić A, Mladenović-Drinić S, Vančetović J, Lazić-Jančić V. Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers. in Genetika. 2006;38(3):227-233. doi:10.2298/GENSR0603227I conv_401 .
Ignjatović-Micić, Dragana, Nikolić, Ana, Mladenović-Drinić, Snežana, Vančetović, Jelena, Lazić-Jančić, Vesna, "Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers" in Genetika, 38, no. 3 (2006):227-233, https://doi.org/10.2298/GENSR0603227I ., conv_401 .