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Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers

Identifikacija tipova citoplazmatične sterilnosti kukuruza primenom specifičnih prajmera mtDNK

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2006
137.pdf (476.1Kb)
Authors
Ignjatović-Micić, Dragana
Nikolić, Ana
Mladenović-Drinić, Snežana
Vančetović, Jelena
Lazić-Jančić, Vesna
Article (Published version)
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Abstract
Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank "Zemun Polje", distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of CMS (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of CMS.
Trideset izvora citoplazmatične muške sterilnosti (CMS) u okviru lokalnih populacija iz Banke gena Instituta za kukuruz "Zemun Polje" je testirano na prisustvo odgovarajućeg tipa citoplazme multipleks PCR metodom, korišćenjem specifičnih prajmera za T, C i S citoplazmu. Kombinovanje tri para prajmera u jednoj PCR reakciji, koji odgovaraju himernim regionima mitohondrijalnih DNK sekvenci specifičnih za svaki tip citoplazme, omogućilo je pouzdanu identifikaciju glavnih tipova sterilne citoplazme. Detektovano je dominantno prisustvo S tipa citoplazme. Za izvore sterilnosti kod kojih nije identifikovan tip sterilne citoplazme (odsustvo PCR trake) postoji realna sumnja da se radi o novim, neidentifikovanim tipovima citoplazmatične muške sterilnosti.
Keywords:
CMS / maize / multiplex PCR
Source:
Genetika, 2006, 38, 3, 227-233
Publisher:
  • Društvo genetičara Srbije, Beograd

DOI: 10.2298/GENSR0603227I

ISSN: 0534-0012

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URI
http://rik.mrizp.rs/handle/123456789/139
Collections
  • Радови истраживача / Researchers' publications
Institution/Community
MRIZP
TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Ana
AU  - Mladenović-Drinić, Snežana
AU  - Vančetović, Jelena
AU  - Lazić-Jančić, Vesna
PY  - 2006
UR  - http://rik.mrizp.rs/handle/123456789/139
AB  - Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank "Zemun Polje", distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of CMS (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of CMS.
AB  - Trideset izvora citoplazmatične muške sterilnosti (CMS) u okviru lokalnih populacija iz Banke gena Instituta za kukuruz "Zemun Polje" je testirano na prisustvo odgovarajućeg tipa citoplazme multipleks PCR metodom, korišćenjem specifičnih prajmera za T, C i S citoplazmu. Kombinovanje tri para prajmera u jednoj PCR reakciji, koji odgovaraju himernim regionima mitohondrijalnih DNK sekvenci specifičnih za svaki tip citoplazme, omogućilo je pouzdanu identifikaciju glavnih tipova sterilne citoplazme. Detektovano je dominantno prisustvo S tipa citoplazme. Za izvore sterilnosti kod kojih nije identifikovan tip sterilne citoplazme (odsustvo PCR trake) postoji realna sumnja da se radi o novim, neidentifikovanim tipovima citoplazmatične muške sterilnosti.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers
T1  - Identifikacija tipova citoplazmatične sterilnosti kukuruza primenom specifičnih prajmera mtDNK
VL  - 38
IS  - 3
SP  - 227
EP  - 233
DO  - 10.2298/GENSR0603227I
UR  - conv_401
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Nikolić, Ana and Mladenović-Drinić, Snežana and Vančetović, Jelena and Lazić-Jančić, Vesna",
year = "2006",
abstract = "Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank "Zemun Polje", distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of CMS (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of CMS., Trideset izvora citoplazmatične muške sterilnosti (CMS) u okviru lokalnih populacija iz Banke gena Instituta za kukuruz "Zemun Polje" je testirano na prisustvo odgovarajućeg tipa citoplazme multipleks PCR metodom, korišćenjem specifičnih prajmera za T, C i S citoplazmu. Kombinovanje tri para prajmera u jednoj PCR reakciji, koji odgovaraju himernim regionima mitohondrijalnih DNK sekvenci specifičnih za svaki tip citoplazme, omogućilo je pouzdanu identifikaciju glavnih tipova sterilne citoplazme. Detektovano je dominantno prisustvo S tipa citoplazme. Za izvore sterilnosti kod kojih nije identifikovan tip sterilne citoplazme (odsustvo PCR trake) postoji realna sumnja da se radi o novim, neidentifikovanim tipovima citoplazmatične muške sterilnosti.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers, Identifikacija tipova citoplazmatične sterilnosti kukuruza primenom specifičnih prajmera mtDNK",
volume = "38",
number = "3",
pages = "227-233",
doi = "10.2298/GENSR0603227I",
url = "conv_401"
}
Ignjatović-Micić, D., Nikolić, A., Mladenović-Drinić, S., Vančetović, J.,& Lazić-Jančić, V.. (2006). Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers. in Genetika
Društvo genetičara Srbije, Beograd., 38(3), 227-233.
https://doi.org/10.2298/GENSR0603227I
conv_401
Ignjatović-Micić D, Nikolić A, Mladenović-Drinić S, Vančetović J, Lazić-Jančić V. Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers. in Genetika. 2006;38(3):227-233.
doi:10.2298/GENSR0603227I
conv_401 .
Ignjatović-Micić, Dragana, Nikolić, Ana, Mladenović-Drinić, Snežana, Vančetović, Jelena, Lazić-Jančić, Vesna, "Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers" in Genetika, 38, no. 3 (2006):227-233,
https://doi.org/10.2298/GENSR0603227I .,
conv_401 .

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