Determination of the fumonisins content in different small grains
Аутори
Savić, IvaNikolić, Milica
Kandić, Vesna
Dodig, Dejan
Obradović, Ana
Ristic, Danijela
Stanković, Slavica
conference contribution (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Fungi of the Fusarium genus belonging to the Liseola section can synthesise
fumonisins of greater or smaller concentrations. Maize is a primary host of these
species. In recent years their presence has been also observed in small grains. The
aim of this study was to observe the concentrations of fumonisins synthesised by
small grains after artificial inoculation. Twelve isolates of the following species were
selected from the collection of fungal cultures of the Maize Research Institute,
Zemun Polje for artificial inoculation: Fusarium verticillioides (6), Fusarium
subglutinans (3) and Fusarium proliferatum (3). The concentrations of synthesised
fumonisins were analysed in four small grains: wheat (Aurelia), barely (Nektar),
triticale (Zenit) and durum (Cosmostar). The artificial inoculation was performed
with the hand sprayer when more than a half of tested plants were in the full-blossom
stage. Inoculation of plants was done in four replications. The amount of i...noculum
(spore concentration was 1x10-6 per 1 ml) was 20 ml per a group of 20 spikes. The
isolate of Fusarium graminearum species was used for spike inoculation in the
positive control, while sterile distilled water was used in the negative control.
Inoculated spikes were covered with wet PVC bags that were removed after 48h.
After harvest, fumonisins were analysed by the ELISA test (Tecna, Italy). According
to obtained results not a single isolate of the observed species synthesised fumonisins
in the barley crop. In the remaining crops, isolates of F. subglutinans species
synthesised fumonisins in low concentrations (0.793-24.949 ppm), while the
corresponding values of isolates of F. proliferatum species were high and ranged
from 35.886 to 60.000 ppm. Isolates of F. verticillioides species had low values in
wheat (2.162-7.925 ppm), while these values in durum were high (29.610-47.174
ppm). The mean values of synthesised fumonisins in the triticale crop were low
(1.357-32.587 ppm).
Кључне речи:
cereals / Fusarium / fumonisinsИзвор:
9. international symposium on agricultural sciences - AgroRes 2020 - Book of abstracts, 2020, 36-Издавач:
- Banja Luka : University of Banja Luka, Faculty of Agriculture