Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation
Аутори
Kovinčić, AnikaMarković, Ksenija
Ristić, Danijela
Babić, Vojka
Petrović, Tanja
Živanović, Tomislav
Kravić, Natalija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A high level of genetic purity in crop varieties must be achieved and maintained for
agronomic performance, encouraging investment and innovation in plant breeding and ensuring that
the improvements in productivity and quality imparted by breeders are delivered to the consumer.
Since the success of hybrid seed production is dependent upon the genetic purity of the parental lines,
in this study, the experimental F1exp maize hybrid and its parental inbreeds were used as a model
system to examine the discriminative power of morphological, biochemical and SSR markers for
seed purity assay. The highest number of off-type plants was estimated by morphological markers.
According to the comparison of prolamins and albumins banding patterns of parental and derived
F1exp seeds, genetic impurities could not be detected. Molecular analysis detected two types of
genetic profile irregularity. Beside its use for verifying varieties of maize, report on umc1545 primer
pair ability to detect n...on-specific bands (i.e., off-types), in both the maternal component and F1exp,
which is the first report on this issue yet, strongly supports the recommendation of this SSR marker
use for more accurate and time-efficient maize hybrids and parental lines genetic pyrity testing
Кључне речи:
phenotypic uniformity / seed storage proteins / SSRs / ultrathin-layer isoelectric focusing / Zea mays L.Извор:
Genes, 2023, 14, 6, -1195Издавач:
- Basel : MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200040 (Институт за кукуруз 'Земун поље', Београд-Земун) (RS-MESTD-inst-2020-200040)