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Proučavanje i čuvanje core kolekcije fitopatogenih gljiva značajnih u poljoprivredi Srbije

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Proučavanje i čuvanje core kolekcije fitopatogenih gljiva značajnih u poljoprivredi Srbije (en)
Проучавање и чување core колекције фитопатогених гљива значајних у пољопривреди Србије (sr)
Proučavanje i čuvanje core kolekcije fitopatogenih gljiva značajnih u poljoprivredi Srbije (sr_RS)
Authors

Publications

The presence of toxigenic Fusarium species and Fusariotoxins deoxynivalenol and zearalenone in winter wheat

Krnjaja, Vesna; Stanković, Slavica; Lević, Jelena

(Institut za stočarstvo, Beograd, 2011)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Lević, Jelena
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/384
AB  - The frequency of fungi and mycotoxin concentrations of deoxynivalenol (DON) and zearalenone (ZON) were studied in winter wheat grains harvested in 2009. The most frequently isolated species belonged to genera Alternaria (81.55%) and Fusarium (12%), followed by Rhizopus spp. (3.75%), Acremoniella spp. (1.15%) and other fungi (Acremonium spp., Arthrinium spp., Aspergillus spp., Bipolaris spp., Chaetomium spp., Nigrospora spp., Penicillium spp. and Ramichloridium spp.) isolated in less than 1%. The following species of the genus Fusarium were identified: F. graminearum (82.50%), F. sporotrichioides (5.42%), F. proliferatum (4.17%), F. subglutinans (4.17%), F. poae (1.66%), F. semitectum (1.25%), and F. verticillioides (0.83%). In 100% of wheat grain samples DON was detected (110-1200 μg kg-1, average 490 μg kg-1), while ZON was detected in 10% of samples and in the lower average of 70 μg kg-1 with the limit values ranging from 60 to 80 μg kg-1. Statistically significant positive correlations were established between the concentration of ZON with the frequency of F. graminearum (r = 0.63) or with the frequency of Fusarium spp. (r = 0.58). A negative insignificant correlation was determined between the DON level and the percentage of present Fusarium species.
AB  - Učestalost gljiva i koncentracija mikotoksina deoksinivalenola (DON) i zearalenona (ZON) je proučavana u zrnu ozime pšenice požnjevenom 2009. godine. Najčešće izolovane vrste gljiva pripadale su rodovima Alternaria (81,55%) i Fusarium (12%), a zatim su sledili Rhizopus spp. (3,75%), Acremoniella spp. (1,15%) i druge gljive (Acremonium spp., Arthrinium spp., Aspergillus spp., Bipolaris spp., Chaetomium spp., Nigrospora spp., Penicillium spp. i Ramichloridium spp.) izolovane u manje od 1%. Unutar roda Fusarium identifikovane su sledeće vrste: F. graminearum (82,50%), F. sporotrichioides (5,42%), F. proliferatum (4,17%), F. subglutinans (4,17%), F. poae (1,66%), F. semitectum (1,25%) i F. verticillioides (0,83%). U 100% uzoraka zrna pšenice DON je bio detektovan u koncentracijama od 110 do 1200 μg kg-1, sa prosečnom koncentracijom od 490 μg kg-1. ZON je bio detektovan u 10% uzoraka u koncentracijama od 60 do 80 μg kg-1, sa prosečnom koncentracijom od 70 μg kg-1. Statistički značajna pozitivna korelacija utvrđena je između koncentracije ZON i frekvencije Fusarium spp. (r = 0.58) i frekvencije F. graminearum (r = 0.63). Negativna korelacija, statistički nesignifikanta, utvrđena je između nivoa DON-a i procentualne zastupljenosti Fusarium vrsta.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - The presence of toxigenic Fusarium species and Fusariotoxins deoxynivalenol and zearalenone in winter wheat
T1  - Prisustvo toksigenih Fusarium vrsta i Fuzariotoksina deoksinivalenola i zearalenona u ozimoj pšenici
VL  - 27
IS  - 1
SP  - 63
EP  - 73
DO  - 10.2298/BAH1101063K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Lević, Jelena",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/384",
abstract = "The frequency of fungi and mycotoxin concentrations of deoxynivalenol (DON) and zearalenone (ZON) were studied in winter wheat grains harvested in 2009. The most frequently isolated species belonged to genera Alternaria (81.55%) and Fusarium (12%), followed by Rhizopus spp. (3.75%), Acremoniella spp. (1.15%) and other fungi (Acremonium spp., Arthrinium spp., Aspergillus spp., Bipolaris spp., Chaetomium spp., Nigrospora spp., Penicillium spp. and Ramichloridium spp.) isolated in less than 1%. The following species of the genus Fusarium were identified: F. graminearum (82.50%), F. sporotrichioides (5.42%), F. proliferatum (4.17%), F. subglutinans (4.17%), F. poae (1.66%), F. semitectum (1.25%), and F. verticillioides (0.83%). In 100% of wheat grain samples DON was detected (110-1200 μg kg-1, average 490 μg kg-1), while ZON was detected in 10% of samples and in the lower average of 70 μg kg-1 with the limit values ranging from 60 to 80 μg kg-1. Statistically significant positive correlations were established between the concentration of ZON with the frequency of F. graminearum (r = 0.63) or with the frequency of Fusarium spp. (r = 0.58). A negative insignificant correlation was determined between the DON level and the percentage of present Fusarium species., Učestalost gljiva i koncentracija mikotoksina deoksinivalenola (DON) i zearalenona (ZON) je proučavana u zrnu ozime pšenice požnjevenom 2009. godine. Najčešće izolovane vrste gljiva pripadale su rodovima Alternaria (81,55%) i Fusarium (12%), a zatim su sledili Rhizopus spp. (3,75%), Acremoniella spp. (1,15%) i druge gljive (Acremonium spp., Arthrinium spp., Aspergillus spp., Bipolaris spp., Chaetomium spp., Nigrospora spp., Penicillium spp. i Ramichloridium spp.) izolovane u manje od 1%. Unutar roda Fusarium identifikovane su sledeće vrste: F. graminearum (82,50%), F. sporotrichioides (5,42%), F. proliferatum (4,17%), F. subglutinans (4,17%), F. poae (1,66%), F. semitectum (1,25%) i F. verticillioides (0,83%). U 100% uzoraka zrna pšenice DON je bio detektovan u koncentracijama od 110 do 1200 μg kg-1, sa prosečnom koncentracijom od 490 μg kg-1. ZON je bio detektovan u 10% uzoraka u koncentracijama od 60 do 80 μg kg-1, sa prosečnom koncentracijom od 70 μg kg-1. Statistički značajna pozitivna korelacija utvrđena je između koncentracije ZON i frekvencije Fusarium spp. (r = 0.58) i frekvencije F. graminearum (r = 0.63). Negativna korelacija, statistički nesignifikanta, utvrđena je između nivoa DON-a i procentualne zastupljenosti Fusarium vrsta.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "The presence of toxigenic Fusarium species and Fusariotoxins deoxynivalenol and zearalenone in winter wheat, Prisustvo toksigenih Fusarium vrsta i Fuzariotoksina deoksinivalenola i zearalenona u ozimoj pšenici",
volume = "27",
number = "1",
pages = "63-73",
doi = "10.2298/BAH1101063K"
}
Krnjaja, V., Stanković, S.,& Lević, J. (2011). Prisustvo toksigenih Fusarium vrsta i Fuzariotoksina deoksinivalenola i zearalenona u ozimoj pšenici.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 27(1), 63-73.
https://doi.org/10.2298/BAH1101063K
Krnjaja V, Stanković S, Lević J. Prisustvo toksigenih Fusarium vrsta i Fuzariotoksina deoksinivalenola i zearalenona u ozimoj pšenici. Biotechnology in Animal Husbandry. 2011;27(1):63-73
Krnjaja Vesna, Stanković Slavica, Lević Jelena, "Prisustvo toksigenih Fusarium vrsta i Fuzariotoksina deoksinivalenola i zearalenona u ozimoj pšenici" 27, no. 1 (2011):63-73,
https://doi.org/10.2298/BAH1101063K .
2

Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids

Lević, Jelena; Petrović, Tijana; Stanković, Slavica; Stanković, Goran; Krnjaja, Vesna

(Wiley-Blackwell, Malden, 2011)

TY  - JOUR
AU  - Lević, Jelena
AU  - Petrović, Tijana
AU  - Stanković, Slavica
AU  - Stanković, Goran
AU  - Krnjaja, Vesna
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/359
AB  - The frequency and incidence of Pyrenochaeta terrestris and symptom type on the roots of each internode of four maize hybrids of different maturity groups were studied 70 days after sowing. The fungus developed in the roots of all developed internodes (from the primary to the sixth or seventh internodes of all tested hybrids). The average frequency and incidence of P. terrestris in the roots of late and medium early maturity hybrids ranged from 29.5 to 55.2% and from 11.8 to 22.7%, respectively. The highest frequency of the fungus was at the 2nd root internode (93.3%), and its greatest incidence was detected in the mesocotyl of the medium early hybrid H-1 (56.9%). Necrosis predominated in the roots of the medium early (i.e. medium late maturity hybrids, 44.5% and 44.3%, respectively), whereas reddish pink symptoms were recorded in the roots of the late hybrids (51% and 42.5%). Because the fungus always produces a distinctive red pigment on carnation leaf agar (CLA) in a light regime, these conditions can be recommended for the reliable identification of P. terrestris, even if pycnidia are not formed. These are the first reports of the successive distribution of the fungus in each maize root internode of different hybrids, as well as the use of CLA medium in the identification of the P. terrestris.
PB  - Wiley-Blackwell, Malden
T2  - Journal of Phytopathology
T1  - Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids
VL  - 159
IS  - 6
SP  - 424
EP  - 428
DO  - 10.1111/j.1439-0434.2011.01784.x
ER  - 
@article{
author = "Lević, Jelena and Petrović, Tijana and Stanković, Slavica and Stanković, Goran and Krnjaja, Vesna",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/359",
abstract = "The frequency and incidence of Pyrenochaeta terrestris and symptom type on the roots of each internode of four maize hybrids of different maturity groups were studied 70 days after sowing. The fungus developed in the roots of all developed internodes (from the primary to the sixth or seventh internodes of all tested hybrids). The average frequency and incidence of P. terrestris in the roots of late and medium early maturity hybrids ranged from 29.5 to 55.2% and from 11.8 to 22.7%, respectively. The highest frequency of the fungus was at the 2nd root internode (93.3%), and its greatest incidence was detected in the mesocotyl of the medium early hybrid H-1 (56.9%). Necrosis predominated in the roots of the medium early (i.e. medium late maturity hybrids, 44.5% and 44.3%, respectively), whereas reddish pink symptoms were recorded in the roots of the late hybrids (51% and 42.5%). Because the fungus always produces a distinctive red pigment on carnation leaf agar (CLA) in a light regime, these conditions can be recommended for the reliable identification of P. terrestris, even if pycnidia are not formed. These are the first reports of the successive distribution of the fungus in each maize root internode of different hybrids, as well as the use of CLA medium in the identification of the P. terrestris.",
publisher = "Wiley-Blackwell, Malden",
journal = "Journal of Phytopathology",
title = "Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids",
volume = "159",
number = "6",
pages = "424-428",
doi = "10.1111/j.1439-0434.2011.01784.x"
}
Lević, J., Petrović, T., Stanković, S., Stanković, G.,& Krnjaja, V. (2011). Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids.
Journal of PhytopathologyWiley-Blackwell, Malden., 159(6), 424-428.
https://doi.org/10.1111/j.1439-0434.2011.01784.x
Lević J, Petrović T, Stanković S, Stanković G, Krnjaja V. Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids. Journal of Phytopathology. 2011;159(6):424-428
Lević Jelena, Petrović Tijana, Stanković Slavica, Stanković Goran, Krnjaja Vesna, "Frequency and Incidence of Pyrenochaeta terrestris in Root Internodes of Different Maize Hybrids" 159, no. 6 (2011):424-428,
https://doi.org/10.1111/j.1439-0434.2011.01784.x .
2
2
3

Fusarium species: The occurrence and the importance in agriculture of Serbia

Lević, Jelena; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra

(Matica srpska, Novi Sad, 2009)

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/267
AB  - Fusarium species have been isolated from over 100 plant species in Serbia. From the economic aspect, they have been and still are the most important for the production and storage of small grains and maize, and are exceptionally important for some other species. Total of 63 species, 35 varieties (var.) and 19 specialised forms (f. sp.) of basic species, particularly of F. oxysporum (4 var. and 12 f. sp.) and F. solani (7 var. and 3 f. sp.) were identified. Species F. langsethiae and F. thapsinum, recently identified, have been isolated from wheat and s o r g h u m seeds, respectively. F. graminearum is the most important pathogen for wheat, barely and maize, while F. poae is also important for wheat and barely. Furthermore, species of the section Liseola (F. verticillioides, F. subglutinans and F. proliferatum) are important for maize and sorghum. In recent years, species of the section Liseola have been increasingly occurring in wheat and barley. The June-October period in Serbia is the most critical period for quality maintenance of stored maize, as the abundance and frequency of fungi, particularly of toxigenic species of the genus Fusarium, are the greatest during that period. In general, there is a lack of data about fusarioses of industrial crops in Serbia. There are mere descriptions of specific cases in which the development of Fusarium species was mostly emphasised by agroecological conditions. The presence of recently determined Fusarium species in kernels of these plant species indicates their importance from the aspect of the yield reduction and grain quality debasement and the mycotoxin contamination. Root rot and plant wilt are characteristic symptoms of fusarioses for forage and vegetable crops, while pathological changes in fruits provoked by Fusarium species are less frequent. F. oxysporum and its specialised forms prevail in these plant species.
AB  - Vrste roda Fusarium su u Srbiji izolovane sa preko 100 biljnih vrsta, a sa ekonomskog aspekta, bile i ostale, najznačajnije za proizvodnju i čuvanje strnih žita i kukuruza. Samo izuzetno su značajne i za neke druge biljne vrste. Ukupno je do sada identifikovano: 63 vrste, 35 varijeteta (var.) i 19 specijalizovanih formi (f. sp.) osnovnih vrsta, posebno vrste F. oxysporum (4 var. i 12 f. sp.) i F. solani (7 var. i 3 f. sp.). F. langsethiae i F. thapsinum su novoidentifikovane vrste izolovane sa zrna pšenice, odnosno sirka. F. graminearum je najznačajniji patogen pšenice, ječma i kukuruza, a F. poae i patogen pšenice i ječma. Vrste iz sekcije Liseola (F. verticillioides, F. subglutinans i F. proliferatum) značajni su i patogeni kukuruza i siraka, mada je poslednjih godina utvrđena sve češća pojava ovih vrsta na zrnu pšenice i ječma. U Srbiji je period jun-oktobar najkritičniji za očuvanje kvaliteta uskladištenog kukuruza jer su brojnost i učestalost gljiva u tom periodu najveće, posebno toksigenih vrsta roda Fusarium (43,5-62,5%). Generalno, fuzarioze industrijskih biljaka su malo proučavane u Srbiji. Opisi su samo pojedinačni slučajevi u kojima je razvoj Fusarium vrsta najčešće potenciran povoljnim agroklimatskih uslovima. Prisustvo Fusarium vrsta na semenu industrijskih biljaka, koje je poslednjih godina sve češće utvrđeno, ukazuje da ove patogene gljive u Srbiji mogu biti značajne sa stanovišta smanjenja prinosa i kvaliteta zrna, posebno u pogledu kontaminiranosti mikotoksinima. Trulež korena i uvenulost biljaka su karakteristični simptomi fuzarioza krmnih i povrtarskih biljaka, dok je pojava patoloških promena na plodovima ređa. Na ovim biljnim vrstama dominiraju F. oxysporum i njene specijalizovane forme.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Fusarium species: The occurrence and the importance in agriculture of Serbia
T1  - Fusarium vrste - pojava i značaj u Srbiji
IS  - 116
SP  - 33
EP  - 48
DO  - 10.2298/ZMSPN0916033L
ER  - 
@article{
author = "Lević, Jelena and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra",
year = "2009",
url = "http://rik.mrizp.rs/handle/123456789/267",
abstract = "Fusarium species have been isolated from over 100 plant species in Serbia. From the economic aspect, they have been and still are the most important for the production and storage of small grains and maize, and are exceptionally important for some other species. Total of 63 species, 35 varieties (var.) and 19 specialised forms (f. sp.) of basic species, particularly of F. oxysporum (4 var. and 12 f. sp.) and F. solani (7 var. and 3 f. sp.) were identified. Species F. langsethiae and F. thapsinum, recently identified, have been isolated from wheat and s o r g h u m seeds, respectively. F. graminearum is the most important pathogen for wheat, barely and maize, while F. poae is also important for wheat and barely. Furthermore, species of the section Liseola (F. verticillioides, F. subglutinans and F. proliferatum) are important for maize and sorghum. In recent years, species of the section Liseola have been increasingly occurring in wheat and barley. The June-October period in Serbia is the most critical period for quality maintenance of stored maize, as the abundance and frequency of fungi, particularly of toxigenic species of the genus Fusarium, are the greatest during that period. In general, there is a lack of data about fusarioses of industrial crops in Serbia. There are mere descriptions of specific cases in which the development of Fusarium species was mostly emphasised by agroecological conditions. The presence of recently determined Fusarium species in kernels of these plant species indicates their importance from the aspect of the yield reduction and grain quality debasement and the mycotoxin contamination. Root rot and plant wilt are characteristic symptoms of fusarioses for forage and vegetable crops, while pathological changes in fruits provoked by Fusarium species are less frequent. F. oxysporum and its specialised forms prevail in these plant species., Vrste roda Fusarium su u Srbiji izolovane sa preko 100 biljnih vrsta, a sa ekonomskog aspekta, bile i ostale, najznačajnije za proizvodnju i čuvanje strnih žita i kukuruza. Samo izuzetno su značajne i za neke druge biljne vrste. Ukupno je do sada identifikovano: 63 vrste, 35 varijeteta (var.) i 19 specijalizovanih formi (f. sp.) osnovnih vrsta, posebno vrste F. oxysporum (4 var. i 12 f. sp.) i F. solani (7 var. i 3 f. sp.). F. langsethiae i F. thapsinum su novoidentifikovane vrste izolovane sa zrna pšenice, odnosno sirka. F. graminearum je najznačajniji patogen pšenice, ječma i kukuruza, a F. poae i patogen pšenice i ječma. Vrste iz sekcije Liseola (F. verticillioides, F. subglutinans i F. proliferatum) značajni su i patogeni kukuruza i siraka, mada je poslednjih godina utvrđena sve češća pojava ovih vrsta na zrnu pšenice i ječma. U Srbiji je period jun-oktobar najkritičniji za očuvanje kvaliteta uskladištenog kukuruza jer su brojnost i učestalost gljiva u tom periodu najveće, posebno toksigenih vrsta roda Fusarium (43,5-62,5%). Generalno, fuzarioze industrijskih biljaka su malo proučavane u Srbiji. Opisi su samo pojedinačni slučajevi u kojima je razvoj Fusarium vrsta najčešće potenciran povoljnim agroklimatskih uslovima. Prisustvo Fusarium vrsta na semenu industrijskih biljaka, koje je poslednjih godina sve češće utvrđeno, ukazuje da ove patogene gljive u Srbiji mogu biti značajne sa stanovišta smanjenja prinosa i kvaliteta zrna, posebno u pogledu kontaminiranosti mikotoksinima. Trulež korena i uvenulost biljaka su karakteristični simptomi fuzarioza krmnih i povrtarskih biljaka, dok je pojava patoloških promena na plodovima ređa. Na ovim biljnim vrstama dominiraju F. oxysporum i njene specijalizovane forme.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Fusarium species: The occurrence and the importance in agriculture of Serbia, Fusarium vrste - pojava i značaj u Srbiji",
number = "116",
pages = "33-48",
doi = "10.2298/ZMSPN0916033L"
}
Lević, J., Stanković, S., Krnjaja, V.,& Bočarov-Stančić, A. (2009). Fusarium vrste - pojava i značaj u Srbiji.
Zbornik Matice srpske za prirodne naukeMatica srpska, Novi Sad.(116), 33-48.
https://doi.org/10.2298/ZMSPN0916033L
Lević J, Stanković S, Krnjaja V, Bočarov-Stančić A. Fusarium vrste - pojava i značaj u Srbiji. Zbornik Matice srpske za prirodne nauke. 2009;(116):33-48
Lević Jelena, Stanković Slavica, Krnjaja Vesna, Bočarov-Stančić Aleksandra, "Fusarium vrste - pojava i značaj u Srbiji", no. 116 (2009):33-48,
https://doi.org/10.2298/ZMSPN0916033L .
19

Ubiquity of toxigenic fungi and mycotoxins in animal feeds in Republic of Serbia

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica

(Institut za stočarstvo, Beograd, 2009)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/249
AB  - Climatic conditions and growing of grain on large areas in Republic of Serbia are suitable for development of numerous toxigenic species, such as Fusarium spp., Aspergillus spp. and Penicillium spp., and resulting from this is frequent incidence of animal feed contamination by their toxic products. In Republic of Serbia, the most frequent fungi determined in animal feed are those from Fusarium genus, as well as their mycotoxins. Of Fusarium species the most frequent one is F. graminearum, and of toxins zearalenon, in maize or wheat grains, which are the main raw material in production of animal feeds. Development of this fungi and bio-synthesis of zearalenon most often depend on period of rains in the third decade of May when wheat is in the blossoming phenostage or on ample precipitation and low temperatures at the end of summer or beginning of autumn during sensitive pheno-stage of maize growing. Aflatoxins are rarely isolated in Republic of Serbia, but there are conditions for their increased presence in imported feed components. Natural occurrence of ochratoxin A is more frequent than incidence of aflatoxins in climatic conditions of Republic of Serbia. Presence of mycotoxins produced by species of genera Fusarium, Aspergillus and Penicillium indicates potential risk of incidence of mycotoxicosis, especially in younger animal categories. For the purpose of protection of health of animals, realization of profit and high productivity in livestock production, it is necessary to explain factors which can cause animal intoxication, and to identify toxins and develop methodology for assessment of the potential toxicity of animal feed.
AB  - Klimatski uslovi i gajenje žita na velikim površinama u Republici Srbiji pogoduju razvoju brojnih toksigenih vrsta, kao što su Fusarium spp., Aspergillus spp. i Penicillium spp., i kao rezultat toga je i česta kontaminacija hrane za životinje njihovim toksičnim produktima. U Republici Srbiji su u hrani za životinje najčešće utvrđene gljive iz roda Fusarium, kao i njihovi mikotoksini. Od Fusarium vrsta najčešća je F. graminearum, a od toksina zearalenon, bilo u zrnu kukuruza ili pšenice koje su najčešće sirovine za stočnu hranu. Razvoj ove gljive i biosinteza zearalenona najčešće zavise od kišnog perioda u trećoj dekadi maja kada je pšenica u fenofazi cvetanja ili od obilnih padavina i niskih temperatura pri kraju leta i početkom jeseni kada je osetljiva fenofaza kukuruza. Aflatoksini su retko izolovani u Republici Srbiji, ali postoje uslovi za veće prisustvo prilikom uvoza komponenti hrane za životinje. Prirodna pojava ohratoksina A je češća od pojave aflatoksina u klimatskim uslovima Srbije. Prisustvo mikotoksina koje produkuju vrste roda Fusarium, Aspergillus i Penicillium ukazuje na potencijalni rizik od pojave mikotoksikoza, posebno za mlađe kategorije životinja. U cilju zaštite zdravlja životinja, ostvarivanja dobiti i visoke produktivnosti u stočarskoj proizvodnji, postoji potreba da se objasne faktori koji mogu prouzrokovati intoksikaciju životinja, kao i da se toksini identifikuju i razvije metodologija za ocenu potencijalne toksičnosti hrane za životinje.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Ubiquity of toxigenic fungi and mycotoxins in animal feeds in Republic of Serbia
T1  - Prisustvo toksigenih vrsta gljiva i mikotoksina u stočnoj hrani u Republici Srbiji
VL  - 25
IS  - 5-6-1
SP  - 477
EP  - 491
DO  - 10.2298/BAH0906477K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica",
year = "2009",
url = "http://rik.mrizp.rs/handle/123456789/249",
abstract = "Climatic conditions and growing of grain on large areas in Republic of Serbia are suitable for development of numerous toxigenic species, such as Fusarium spp., Aspergillus spp. and Penicillium spp., and resulting from this is frequent incidence of animal feed contamination by their toxic products. In Republic of Serbia, the most frequent fungi determined in animal feed are those from Fusarium genus, as well as their mycotoxins. Of Fusarium species the most frequent one is F. graminearum, and of toxins zearalenon, in maize or wheat grains, which are the main raw material in production of animal feeds. Development of this fungi and bio-synthesis of zearalenon most often depend on period of rains in the third decade of May when wheat is in the blossoming phenostage or on ample precipitation and low temperatures at the end of summer or beginning of autumn during sensitive pheno-stage of maize growing. Aflatoxins are rarely isolated in Republic of Serbia, but there are conditions for their increased presence in imported feed components. Natural occurrence of ochratoxin A is more frequent than incidence of aflatoxins in climatic conditions of Republic of Serbia. Presence of mycotoxins produced by species of genera Fusarium, Aspergillus and Penicillium indicates potential risk of incidence of mycotoxicosis, especially in younger animal categories. For the purpose of protection of health of animals, realization of profit and high productivity in livestock production, it is necessary to explain factors which can cause animal intoxication, and to identify toxins and develop methodology for assessment of the potential toxicity of animal feed., Klimatski uslovi i gajenje žita na velikim površinama u Republici Srbiji pogoduju razvoju brojnih toksigenih vrsta, kao što su Fusarium spp., Aspergillus spp. i Penicillium spp., i kao rezultat toga je i česta kontaminacija hrane za životinje njihovim toksičnim produktima. U Republici Srbiji su u hrani za životinje najčešće utvrđene gljive iz roda Fusarium, kao i njihovi mikotoksini. Od Fusarium vrsta najčešća je F. graminearum, a od toksina zearalenon, bilo u zrnu kukuruza ili pšenice koje su najčešće sirovine za stočnu hranu. Razvoj ove gljive i biosinteza zearalenona najčešće zavise od kišnog perioda u trećoj dekadi maja kada je pšenica u fenofazi cvetanja ili od obilnih padavina i niskih temperatura pri kraju leta i početkom jeseni kada je osetljiva fenofaza kukuruza. Aflatoksini su retko izolovani u Republici Srbiji, ali postoje uslovi za veće prisustvo prilikom uvoza komponenti hrane za životinje. Prirodna pojava ohratoksina A je češća od pojave aflatoksina u klimatskim uslovima Srbije. Prisustvo mikotoksina koje produkuju vrste roda Fusarium, Aspergillus i Penicillium ukazuje na potencijalni rizik od pojave mikotoksikoza, posebno za mlađe kategorije životinja. U cilju zaštite zdravlja životinja, ostvarivanja dobiti i visoke produktivnosti u stočarskoj proizvodnji, postoji potreba da se objasne faktori koji mogu prouzrokovati intoksikaciju životinja, kao i da se toksini identifikuju i razvije metodologija za ocenu potencijalne toksičnosti hrane za životinje.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Ubiquity of toxigenic fungi and mycotoxins in animal feeds in Republic of Serbia, Prisustvo toksigenih vrsta gljiva i mikotoksina u stočnoj hrani u Republici Srbiji",
volume = "25",
number = "5-6-1",
pages = "477-491",
doi = "10.2298/BAH0906477K"
}
Krnjaja, V., Lević, J.,& Stanković, S. (2009). Prisustvo toksigenih vrsta gljiva i mikotoksina u stočnoj hrani u Republici Srbiji.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 25(5-6-1), 477-491.
https://doi.org/10.2298/BAH0906477K
Krnjaja V, Lević J, Stanković S. Prisustvo toksigenih vrsta gljiva i mikotoksina u stočnoj hrani u Republici Srbiji. Biotechnology in Animal Husbandry. 2009;25(5-6-1):477-491
Krnjaja Vesna, Lević Jelena, Stanković Slavica, "Prisustvo toksigenih vrsta gljiva i mikotoksina u stočnoj hrani u Republici Srbiji" 25, no. 5-6-1 (2009):477-491,
https://doi.org/10.2298/BAH0906477K .
4

Variability of pathogenicity of Fusarium spp. originating from maize and wheat grains

Tančić, Sonja; Stanković, Slavica; Lević, Jelena

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2009)

TY  - JOUR
AU  - Tančić, Sonja
AU  - Stanković, Slavica
AU  - Lević, Jelena
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/259
AB  - Differences in the pathogenicity of 93 isolates of seven species belonging to the genus Fusarium (F. graminearum, F. verticillioides, F. proliferatum, F. subglutinans, F. sporotrichioides, F. semitectum and F. equiseti), originating from maize kernels (61) and wheat grains (32), were examined based on the germination percentage of inoculated seeds. The studied species demonstrated inter- and intraspecies variability regarding the effects on maize seed germination. On the average, the greatest germination reduction was found in seeds inoculated with the spore suspensions of F. sporotrichioides and F. graminearum. A similar reduction was detected in seeds inoculated with F. proliferatum and F. subglutinans. The effect of F. subglutinans on seed germination reduction was higher compared to the two latter species, while the effects of F. semitectum and F. equiseti were smallest. The majority of isolates were of moderate pathogenicity, while the lowest number of isolates was either very pathogenic (7) or apathogenic (10). Pathogenicity of the isolates originating from wheat grains was generally lower than the pathogenicity of isolates originating from maize kernels, with the exception of F. sporotrichioides.
AB  - Razlike u patogenost 93 izolata sedam vrsta roda Fusarium (F. graminearum, F. verticillioides, F. proliferatum, F. subglutinans, F. sporotrichioides, F. semitectum i F. equiseti), poreklom iz zrna kukuruza (61) i pšenice (32), proučavane su na osnovu procenta klijavosti inokulisanog semena. Ispitivane vrste ispoljile su inter- i intraspecijsku varijabilnost u pogledu uticaja na klijavost semena kukuruza. U proseku, najveće smanjenje klijavosti je utvrđeno kod inokulacije semena suspenzijom spora F. sporotrichioides i F. graminearum, a zatim, približno isto, kod inokulacija semena pomoću F. proliferatum i F. subglutinans. F. verticillioides je, u poređenju sa ove dve poslednje vrste, značajnije uticala na smanjenje klijavosti semena, dok su najmanje uticale vrste F. semitectum i F. equiseti. Većina izolata je bila srednje patogenosti, a najmanji broj je bio jako patogen (7) ili apatogen (10). Izolati poreklom iz zrna pšenice bili su slabije patogenosti od izolata poreklom iz zrna kukuruza, sa izuzetkom F. sporotrichioides.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Variability of pathogenicity of Fusarium spp. originating from maize and wheat grains
T1  - Varijabilnost patogenih svojstava Fusarium spp. poreklom iz zrna kukuruza i pšenice
VL  - 24
IS  - 4
SP  - 259
EP  - 269
DO  - 10.2298/PIF0904259T
ER  - 
@article{
author = "Tančić, Sonja and Stanković, Slavica and Lević, Jelena",
year = "2009",
url = "http://rik.mrizp.rs/handle/123456789/259",
abstract = "Differences in the pathogenicity of 93 isolates of seven species belonging to the genus Fusarium (F. graminearum, F. verticillioides, F. proliferatum, F. subglutinans, F. sporotrichioides, F. semitectum and F. equiseti), originating from maize kernels (61) and wheat grains (32), were examined based on the germination percentage of inoculated seeds. The studied species demonstrated inter- and intraspecies variability regarding the effects on maize seed germination. On the average, the greatest germination reduction was found in seeds inoculated with the spore suspensions of F. sporotrichioides and F. graminearum. A similar reduction was detected in seeds inoculated with F. proliferatum and F. subglutinans. The effect of F. subglutinans on seed germination reduction was higher compared to the two latter species, while the effects of F. semitectum and F. equiseti were smallest. The majority of isolates were of moderate pathogenicity, while the lowest number of isolates was either very pathogenic (7) or apathogenic (10). Pathogenicity of the isolates originating from wheat grains was generally lower than the pathogenicity of isolates originating from maize kernels, with the exception of F. sporotrichioides., Razlike u patogenost 93 izolata sedam vrsta roda Fusarium (F. graminearum, F. verticillioides, F. proliferatum, F. subglutinans, F. sporotrichioides, F. semitectum i F. equiseti), poreklom iz zrna kukuruza (61) i pšenice (32), proučavane su na osnovu procenta klijavosti inokulisanog semena. Ispitivane vrste ispoljile su inter- i intraspecijsku varijabilnost u pogledu uticaja na klijavost semena kukuruza. U proseku, najveće smanjenje klijavosti je utvrđeno kod inokulacije semena suspenzijom spora F. sporotrichioides i F. graminearum, a zatim, približno isto, kod inokulacija semena pomoću F. proliferatum i F. subglutinans. F. verticillioides je, u poređenju sa ove dve poslednje vrste, značajnije uticala na smanjenje klijavosti semena, dok su najmanje uticale vrste F. semitectum i F. equiseti. Većina izolata je bila srednje patogenosti, a najmanji broj je bio jako patogen (7) ili apatogen (10). Izolati poreklom iz zrna pšenice bili su slabije patogenosti od izolata poreklom iz zrna kukuruza, sa izuzetkom F. sporotrichioides.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Variability of pathogenicity of Fusarium spp. originating from maize and wheat grains, Varijabilnost patogenih svojstava Fusarium spp. poreklom iz zrna kukuruza i pšenice",
volume = "24",
number = "4",
pages = "259-269",
doi = "10.2298/PIF0904259T"
}
Tančić, S., Stanković, S.,& Lević, J. (2009). Varijabilnost patogenih svojstava Fusarium spp. poreklom iz zrna kukuruza i pšenice.
Pesticidi i fitomedicinaInstitut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 24(4), 259-269.
https://doi.org/10.2298/PIF0904259T
Tančić S, Stanković S, Lević J. Varijabilnost patogenih svojstava Fusarium spp. poreklom iz zrna kukuruza i pšenice. Pesticidi i fitomedicina. 2009;24(4):259-269
Tančić Sonja, Stanković Slavica, Lević Jelena, "Varijabilnost patogenih svojstava Fusarium spp. poreklom iz zrna kukuruza i pšenice" 24, no. 4 (2009):259-269,
https://doi.org/10.2298/PIF0904259T .

Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions

Bočarov-Stančić, Aleksandra; Lević, Jelena; Stanković, Slavica; Stanišić, Mladen M.; Bilek, Saša O.

(Matica srpska, Novi Sad, 2009)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Stanišić, Mladen M.
AU  - Bilek, Saša O.
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/255
AB  - Toxicological investigations encompassed two cultures of Fusarium graminearum: (i) D2 isolate, originating from air was obtained on Sabouraud medium during a routine control of laboratory sterility conditions at the Department of Microbiology of the Center for Bio-Ecology in 2006, and (ii) GZ-LES control isolate, a well known producer of zearalenone (ZON) and deoxynivalenol (DON), was isolated from maize kernel collected at Leskovac in 1975. Preliminary analysis of fungal potential for the production of DON and ZON were performed by the modified rapid screening method of Filtenborg et al. (1983). Dynamics of DON and ZON biosynthesis was tested under different conditions of isolate cultivation: (i) in a basic liquid semi-synthetic medium with 2% yeast extract and 15% sucrose, pH 6.5 (YES), (ii) in broth with same concentrations of yeast extract and sucrose supplemented with 0.23 mg/l ZnSO4 x 5 H2O, pH 6.5 (YESZn) and (iii) on natural solid substrates such as wet sterilized maize and rice kernels. The quantitative determination of DON and ZON was performed in both liquid and natural solid substrates with thin-layer chromatographic methods (TLCs). The maximum yield of DON was recorded after three weeks of cultivation on maize kernels at 27±1°C. Contrary to the D2 isolate, which did not show the potential for the DON biosynthesis, the control isolate GZ-LES produced 645.6 ppb of the same type B trichothecene under previously mentioned conditions. The ZON biosynthesis by the isolate D2 (1.2 ppb) was observed after 2 weeks of the stationary cultivation in YES and YESZn at room temperature (17-19°C). The same isolate produced 0.74 ppb and 17.35 ppb ZON on maize and rice kernels after only 7 and 28 days of cultivation at the room temperature ranging from 17 to 19°C and from 15 to 23°C, respectively.
AB  - Ispitivanjem su bili obuhvaćeni novoizolovana kultura F. graminearum (D2) neispitanog toksikološkog profila i kontrolni izolat iste vrste gljive (GZ-LES) dobro poznat proizvođač deoksinivalenola (DON) i zearalenona (ZON). Preliminarne analize DON-a i ZON-a su izvršene prema modifikovanoj metodi Filtenborga i sar. (1983). Dinamika biosinteze DON-a i ZON-a je praćena gajenjem izolata gljiva u/na četiri različita tipa podloge: tečnoj polusintetičkoj podlozi sa 2% ekstrakta kvasca i 15% saharoze (YES pH 6,5), podlozi istog sastava sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O (YESZn pH 6,5) i sterilisanim vlažnim zrnima kukuruza i pirinča. Kvantitativno određivanje DON-a u kulturi izolata gljiva gajenih u tečnoj podlozi je izvršeno tankoslojnom hromatografijom prema Cvetniću i sar. (2005), a u čvrstoj podlozi primenom postupka autora Pepeljnjaka i Babića (1991). Potencijal za biosintezu ZON-a kod izolata gajenih u tečnoj podlozi je određivan postupkom tankoslojne hromatografije prema Bočarov - Stančić i sar. (2003), a izolata gajenih na čvrstoj podlozi prema Balzeru i sar. (1978). Maksimalna koncentracija DON-a (645,6 ppb) je determinisana posle tri nedelje kultivacije kontrolnog izolata F. graminearum GZ-LES na zrnu kukuruza i pri 27±1°S. Za izolat iste vrste gljive D2 je utvrđeno da ne poseduje sposobnost biosinteze DON-a, s obzirom da nije proizvodio ovaj trihotecen tipa B ni u jednom od testiranih uslova kultivacije. Proizvodnja ZON-a je konstatovana kod izolata D2 (1,2 ppb, odnosno 1,5 ppb) posle dve nedelje stacionarne kultivacije u tečnim podlogama (YES i YESZn) na sobnoj temperaturi od 17 do 19°C. Na prirodnim čvrstim supstratima (zrno kukuruza i pirinča) isti izolat je biosintetisao ZON već posle sedam dana kultivacije na zrnu kukuruza i sobnoj temperaturi od 17 do 19°C (0,74 ppb) ili posle 28 dana kultivacije na zrnu pirinča i sobnoj temperaturi od 15 do 23°C (17,4 ppb).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions
T1  - Dinamika proizvodnje deoksinivalenola i zearalenona kod izolata Fusarium graminearum u laboratorijskim uslovima
IS  - 116
SP  - 15
EP  - 24
DO  - 10.2298/ZMSPN0916015B
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Stanković, Slavica and Stanišić, Mladen M. and Bilek, Saša O.",
year = "2009",
url = "http://rik.mrizp.rs/handle/123456789/255",
abstract = "Toxicological investigations encompassed two cultures of Fusarium graminearum: (i) D2 isolate, originating from air was obtained on Sabouraud medium during a routine control of laboratory sterility conditions at the Department of Microbiology of the Center for Bio-Ecology in 2006, and (ii) GZ-LES control isolate, a well known producer of zearalenone (ZON) and deoxynivalenol (DON), was isolated from maize kernel collected at Leskovac in 1975. Preliminary analysis of fungal potential for the production of DON and ZON were performed by the modified rapid screening method of Filtenborg et al. (1983). Dynamics of DON and ZON biosynthesis was tested under different conditions of isolate cultivation: (i) in a basic liquid semi-synthetic medium with 2% yeast extract and 15% sucrose, pH 6.5 (YES), (ii) in broth with same concentrations of yeast extract and sucrose supplemented with 0.23 mg/l ZnSO4 x 5 H2O, pH 6.5 (YESZn) and (iii) on natural solid substrates such as wet sterilized maize and rice kernels. The quantitative determination of DON and ZON was performed in both liquid and natural solid substrates with thin-layer chromatographic methods (TLCs). The maximum yield of DON was recorded after three weeks of cultivation on maize kernels at 27±1°C. Contrary to the D2 isolate, which did not show the potential for the DON biosynthesis, the control isolate GZ-LES produced 645.6 ppb of the same type B trichothecene under previously mentioned conditions. The ZON biosynthesis by the isolate D2 (1.2 ppb) was observed after 2 weeks of the stationary cultivation in YES and YESZn at room temperature (17-19°C). The same isolate produced 0.74 ppb and 17.35 ppb ZON on maize and rice kernels after only 7 and 28 days of cultivation at the room temperature ranging from 17 to 19°C and from 15 to 23°C, respectively., Ispitivanjem su bili obuhvaćeni novoizolovana kultura F. graminearum (D2) neispitanog toksikološkog profila i kontrolni izolat iste vrste gljive (GZ-LES) dobro poznat proizvođač deoksinivalenola (DON) i zearalenona (ZON). Preliminarne analize DON-a i ZON-a su izvršene prema modifikovanoj metodi Filtenborga i sar. (1983). Dinamika biosinteze DON-a i ZON-a je praćena gajenjem izolata gljiva u/na četiri različita tipa podloge: tečnoj polusintetičkoj podlozi sa 2% ekstrakta kvasca i 15% saharoze (YES pH 6,5), podlozi istog sastava sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O (YESZn pH 6,5) i sterilisanim vlažnim zrnima kukuruza i pirinča. Kvantitativno određivanje DON-a u kulturi izolata gljiva gajenih u tečnoj podlozi je izvršeno tankoslojnom hromatografijom prema Cvetniću i sar. (2005), a u čvrstoj podlozi primenom postupka autora Pepeljnjaka i Babića (1991). Potencijal za biosintezu ZON-a kod izolata gajenih u tečnoj podlozi je određivan postupkom tankoslojne hromatografije prema Bočarov - Stančić i sar. (2003), a izolata gajenih na čvrstoj podlozi prema Balzeru i sar. (1978). Maksimalna koncentracija DON-a (645,6 ppb) je determinisana posle tri nedelje kultivacije kontrolnog izolata F. graminearum GZ-LES na zrnu kukuruza i pri 27±1°S. Za izolat iste vrste gljive D2 je utvrđeno da ne poseduje sposobnost biosinteze DON-a, s obzirom da nije proizvodio ovaj trihotecen tipa B ni u jednom od testiranih uslova kultivacije. Proizvodnja ZON-a je konstatovana kod izolata D2 (1,2 ppb, odnosno 1,5 ppb) posle dve nedelje stacionarne kultivacije u tečnim podlogama (YES i YESZn) na sobnoj temperaturi od 17 do 19°C. Na prirodnim čvrstim supstratima (zrno kukuruza i pirinča) isti izolat je biosintetisao ZON već posle sedam dana kultivacije na zrnu kukuruza i sobnoj temperaturi od 17 do 19°C (0,74 ppb) ili posle 28 dana kultivacije na zrnu pirinča i sobnoj temperaturi od 15 do 23°C (17,4 ppb).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions, Dinamika proizvodnje deoksinivalenola i zearalenona kod izolata Fusarium graminearum u laboratorijskim uslovima",
number = "116",
pages = "15-24",
doi = "10.2298/ZMSPN0916015B"
}
Bočarov-Stančić, A., Lević, J., Stanković, S., Stanišić, M. M.,& Bilek, S. O. (2009). Dinamika proizvodnje deoksinivalenola i zearalenona kod izolata Fusarium graminearum u laboratorijskim uslovima.
Zbornik Matice srpske za prirodne naukeMatica srpska, Novi Sad.(116), 15-24.
https://doi.org/10.2298/ZMSPN0916015B
Bočarov-Stančić A, Lević J, Stanković S, Stanišić MM, Bilek SO. Dinamika proizvodnje deoksinivalenola i zearalenona kod izolata Fusarium graminearum u laboratorijskim uslovima. Zbornik Matice srpske za prirodne nauke. 2009;(116):15-24
Bočarov-Stančić Aleksandra, Lević Jelena, Stanković Slavica, Stanišić Mladen M., Bilek Saša O., "Dinamika proizvodnje deoksinivalenola i zearalenona kod izolata Fusarium graminearum u laboratorijskim uslovima", no. 116 (2009):15-24,
https://doi.org/10.2298/ZMSPN0916015B .
2

Investigation of toxigenic potential of fungal species by the use of simple screening method

Bočarov-Stančić, Aleksandra; Lević, Jelena; Dimić, Gordana R.; Stanković, Slavica; Salma, Nataša M.

(Matica srpska, Novi Sad, 2009)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lević, Jelena
AU  - Dimić, Gordana R.
AU  - Stanković, Slavica
AU  - Salma, Nataša M.
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/281
AB  - Potential for the biosynthesis of aflatoxin B1 (AFLB1), ochratoxin A (OTA), diacetoxyscirpenol (DAS), T-2 toxin (T2), and zearalenone (ZON) was investigated in different fungal species belonging to the genera: Aspergillus, Fusarium and Penicillium. The majority of investigated isolates originated from cereal grains, crushed oil soybean seed and fodder mixtures. The simple screening method developed by Filtenborg et al. (1983) was applied with few modifications concerning the type of the medium and cultivation temperature. In order to optimise the biosynthetic conditions for different mycotoxins, the following control cultures, known as mycotin producers were used: OTA - A. ochraceus CBS 108.08, DAS - F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) and ZON - F. graminearum (GZ-LES). The fungi were cultivated on the standard medium (YESA - 2% yeast extract, 15% sucrose and 2% agar, pH 6.5), three modifications of the basic medium (YESAZn - the standard medium supplemented with 0.23 mg/l ZnSO4 x 5 H2O; PPSA - the medium in which yeast extract was replaced with peptone-1; PPSAZn - the medium in which yeast extract was replaced with peptone-1 and supplemented with 0.23 mg/l ZnSO4 x 5 H2O), and the potato-dextrose agar (PDA). The earlier biosynthesis of tested mycotoxins was recorded under the following cultivation conditions of fungal species: AFLB1 - after 14 days on PDA at 27±1°C, OTA - after 10 days on YESA and YESAZn at 27±1°C, DAS - after 10 days on PPSA and PPSAZn at 27±1°C, T2 - after 7 days on PPSAZn and PPSA at room temperature (20-24°C), and ZON - after 1 week on YESA and YESAZn at room temperature (21-24°C).
AB  - Potencijal za biosintezu aflatoksina B1 (AFLB1), ohratoksina A (OTA), diacetoksiscirpenola (DAS), T-2 toksina (T2) i zearalenona je ispitan kod izolata gljiva koje pripadaju rodovima Aspergillus, Fusarium i Penicillium. Izolati su uglavnom bili poreklom sa zrna žitarica i stočne hrane. Primenjena je jednostavna trijažna metoda F i l t e n b o r g-a i sar. (1983) kod koje su izvršene izvesne modifikacije u smislu tipa podloge i temperature kultivacije gljiva. U cilju optimizacije uslova za testiranje toksigenog profila odabranih gljivičnih izolata upotrebljene su kontrolne kulture za koje je prethodno dokazano da su proizvođači sledećih mikotoksina: OTA-a A. ochraceus CBS 108.08, DAS-a F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) i ZON-a F. graminearum (GZ-LES). Gljive su gajene na standardnoj podlozi (EKSA - 2% ekstrakta kvasca i 15% saharoze, pH 6,5), tri modifikacije osnovne podloge (EKSAZn-standardna podloga sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O; PPSA - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1; PPSAZn - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1 i kojoj je dodato 0,23 mg/l ZnSO4 x 5 H2O; pH 6,5) i krompir dekstroznoj podlozi (pH 6,5). Biosinteza ispitanih mikotoksina je najranije konstatovana pri sledećim uslovima gajenja gljiva: AFLB1 - posle 14 dana kultivacije na KDA i 27±1°C, OTA - posle 10 dana kultivacije na EKSA i EKSAZn i 27±1°C, DAS-a posle 10 dana kultivacije na PPSA i PPSAZn na 27±1°C, T2 - posle 7 dana na PPSA i PPSAZn i sobnoj temperaturi (20-24°C), i ZON-a posle nedelju dana na EKSA i EKSAZn i sobnoj temperaturi (21-24°C).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Investigation of toxigenic potential of fungal species by the use of simple screening method
T1  - Ispitivanje toksigenog potencijala gljiva primenom jednostavnog trijažnog metoda
IS  - 116
SP  - 25
EP  - 32
DO  - 10.2298/ZMSPN0916025B
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lević, Jelena and Dimić, Gordana R. and Stanković, Slavica and Salma, Nataša M.",
year = "2009",
url = "http://rik.mrizp.rs/handle/123456789/281",
abstract = "Potential for the biosynthesis of aflatoxin B1 (AFLB1), ochratoxin A (OTA), diacetoxyscirpenol (DAS), T-2 toxin (T2), and zearalenone (ZON) was investigated in different fungal species belonging to the genera: Aspergillus, Fusarium and Penicillium. The majority of investigated isolates originated from cereal grains, crushed oil soybean seed and fodder mixtures. The simple screening method developed by Filtenborg et al. (1983) was applied with few modifications concerning the type of the medium and cultivation temperature. In order to optimise the biosynthetic conditions for different mycotoxins, the following control cultures, known as mycotin producers were used: OTA - A. ochraceus CBS 108.08, DAS - F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) and ZON - F. graminearum (GZ-LES). The fungi were cultivated on the standard medium (YESA - 2% yeast extract, 15% sucrose and 2% agar, pH 6.5), three modifications of the basic medium (YESAZn - the standard medium supplemented with 0.23 mg/l ZnSO4 x 5 H2O; PPSA - the medium in which yeast extract was replaced with peptone-1; PPSAZn - the medium in which yeast extract was replaced with peptone-1 and supplemented with 0.23 mg/l ZnSO4 x 5 H2O), and the potato-dextrose agar (PDA). The earlier biosynthesis of tested mycotoxins was recorded under the following cultivation conditions of fungal species: AFLB1 - after 14 days on PDA at 27±1°C, OTA - after 10 days on YESA and YESAZn at 27±1°C, DAS - after 10 days on PPSA and PPSAZn at 27±1°C, T2 - after 7 days on PPSAZn and PPSA at room temperature (20-24°C), and ZON - after 1 week on YESA and YESAZn at room temperature (21-24°C)., Potencijal za biosintezu aflatoksina B1 (AFLB1), ohratoksina A (OTA), diacetoksiscirpenola (DAS), T-2 toksina (T2) i zearalenona je ispitan kod izolata gljiva koje pripadaju rodovima Aspergillus, Fusarium i Penicillium. Izolati su uglavnom bili poreklom sa zrna žitarica i stočne hrane. Primenjena je jednostavna trijažna metoda F i l t e n b o r g-a i sar. (1983) kod koje su izvršene izvesne modifikacije u smislu tipa podloge i temperature kultivacije gljiva. U cilju optimizacije uslova za testiranje toksigenog profila odabranih gljivičnih izolata upotrebljene su kontrolne kulture za koje je prethodno dokazano da su proizvođači sledećih mikotoksina: OTA-a A. ochraceus CBS 108.08, DAS-a F. semitectum (SL-B i SL-C), T2 - F. sporotrichioides (ITM-391, M-1-1, R-2301) i ZON-a F. graminearum (GZ-LES). Gljive su gajene na standardnoj podlozi (EKSA - 2% ekstrakta kvasca i 15% saharoze, pH 6,5), tri modifikacije osnovne podloge (EKSAZn-standardna podloga sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O; PPSA - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1; PPSAZn - podloga u kojoj je ekstrakt kvasca zamenjen peptonom-1 i kojoj je dodato 0,23 mg/l ZnSO4 x 5 H2O; pH 6,5) i krompir dekstroznoj podlozi (pH 6,5). Biosinteza ispitanih mikotoksina je najranije konstatovana pri sledećim uslovima gajenja gljiva: AFLB1 - posle 14 dana kultivacije na KDA i 27±1°C, OTA - posle 10 dana kultivacije na EKSA i EKSAZn i 27±1°C, DAS-a posle 10 dana kultivacije na PPSA i PPSAZn na 27±1°C, T2 - posle 7 dana na PPSA i PPSAZn i sobnoj temperaturi (20-24°C), i ZON-a posle nedelju dana na EKSA i EKSAZn i sobnoj temperaturi (21-24°C).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Investigation of toxigenic potential of fungal species by the use of simple screening method, Ispitivanje toksigenog potencijala gljiva primenom jednostavnog trijažnog metoda",
number = "116",
pages = "25-32",
doi = "10.2298/ZMSPN0916025B"
}
Bočarov-Stančić, A., Lević, J., Dimić, G. R., Stanković, S.,& Salma, N. M. (2009). Ispitivanje toksigenog potencijala gljiva primenom jednostavnog trijažnog metoda.
Zbornik Matice srpske za prirodne naukeMatica srpska, Novi Sad.(116), 25-32.
https://doi.org/10.2298/ZMSPN0916025B
Bočarov-Stančić A, Lević J, Dimić GR, Stanković S, Salma NM. Ispitivanje toksigenog potencijala gljiva primenom jednostavnog trijažnog metoda. Zbornik Matice srpske za prirodne nauke. 2009;(116):25-32
Bočarov-Stančić Aleksandra, Lević Jelena, Dimić Gordana R., Stanković Slavica, Salma Nataša M., "Ispitivanje toksigenog potencijala gljiva primenom jednostavnog trijažnog metoda", no. 116 (2009):25-32,
https://doi.org/10.2298/ZMSPN0916025B .
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